@article{KressJarrinThuroffetal.2004,
  author    = {Kress, H. and Jarrin, A. and Thuroff, E. and Saunders, R. and Weise, C. and Schmidt am Busch, Marcel and Knapp, E. W. and Wedde, M. and Vilcinskas, Andreas},
  title     = {A Kunitz type protease inhibitor related protein is synthesized in Drosophila prepupal salivary glands and released into the moulting fluid during pupation},
  issn      = {0965-1748},
  year      = {2004},
  abstract  = {From the Drosophila virilis late puff region 31C, we microcloned two neighbouring genes, Kil-1 and Kil-2, that encode putative Kunitz serine protease inhibitor like proteins. The Kil-1 gene is expressed exclusively in prepupal salivary glands. Using a size mutant of the KIL-1 protein and MALDI-TOF analysis, we demonstrate that during pupation this protein is released from the prepupal salivary glands into the pupation fluid covering the surface of the pupa. 3-D- structure predictions are consistent with the known crystal structure of the human Kunitz type protease inhibitor 2KNT. This is the first experimental proof for the extra-corporal presence of a distinct Drosophila prepupal salivary gland protein. Possible functions of KIL-1 in the context of the control of proteolytic activities in the pupation fluid are discussed. (C) 2004 Elsevier Ltd. All rights reserved},
  language  = {en}
}