@article{ZorHeiskanenCavigliaetal.2014,
  author    = {Zor, K. and Heiskanen, A. and Caviglia, Claudia and Vergani, M. and Landini, E. and Shah, F. and Carminati, Marco and Martinez-Serrano, A. and Ramos Moreno, T. and Kokaia, M. and Benayahu, Dafna and Keresztes, Zs. and Papkovsky, D. and Wollenberger, Ursula and Svendsen, W. E. and Dimaki, M. and Ferrari, G. and Raiteri, R. and Sampietro, M. and Dufva, M. and Emneus, Jenny},
  title     = {A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection},
  series = {RSC Advances},
  volume    = {4},
  journal   = {RSC Advances},
  number    = {109},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  issn      = {2046-2069},
  doi       = {10.1039/c4ra12632g},
  pages     = {63761 -- 63771},
  year      = {2014},
  abstract  = {Downscaling of microfluidic cell culture and detection devices for electrochemical monitoring has mostly focused on miniaturization of the microfluidic chips which are often designed for specific applications and therefore lack functional flexibility. We present a compact microfluidic cell culture and electrochemical analysis platform with in-built fluid handling and detection, enabling complete cell based assays comprising on-line electrode cleaning, sterilization, surface functionalization, cell seeding, cultivation and electrochemical real-time monitoring of cellular dynamics. To demonstrate the versatility and multifunctionality of the platform, we explored amperometric monitoring of intracellular redox activity in yeast (Saccharomyces cerevisiae) and detection of exocytotically released dopamine from rat pheochromocytoma cells (PC12). Electrochemical impedance spectroscopy was used in both applications for monitoring cell sedimentation and adhesion as well as proliferation in the case of PC12 cells. The influence of flow rate on the signal amplitude in the detection of redox metabolism as well as the effect of mechanical stimulation on dopamine release were demonstrated using the programmable fluid handling capability. The here presented platform is aimed at applications utilizing cell based assays, ranging from e.g. monitoring of drug effects in pharmacological studies, characterization of neural stem cell differentiation, and screening of genetically modified microorganisms to environmental monitoring.},
  language  = {en}
}
@article{VerganiCarminatiFerrarietal.2012,
  author    = {Vergani, Marco and Carminati, Marco and Ferrari, Giorgio and Landini, Ettore and Caviglia, Claudia and Heiskanen, Arto and Comminges, Clement and Zor, Kinga and Sabourin, David and Dufva, Martin and Dimaki, Maria and Raiteri, Roberto and Wollenberger, Ursula and Emneus, Jenny and Sampietro, Marco},
  title     = {Multichannel bipotentiostat integrated with a microfluidic platform for electrochemical real-time monitoring of cell cultures},
  series = {IEEE Transactions on biomedical circuits and systems},
  volume    = {6},
  journal   = {IEEE Transactions on biomedical circuits and systems},
  number    = {5},
  publisher = {Inst. of Electr. and Electronics Engineers},
  address   = {Piscataway},
  issn      = {1932-4545},
  doi       = {10.1109/TBCAS.2012.2187783},
  pages     = {498 -- 507},
  year      = {2012},
  abstract  = {An electrochemical detection system specifically designed for multi-parameter real-time monitoring of stem cell culturing/differentiation in a microfluidic system is presented. It is composed of a very compact 24-channel electronic board, compatible with arrays of microelectrodes and coupled to a microfluidic cell culture system. A versatile data acquisition software enables performing amperometry, cyclic voltammetry and impedance spectroscopy in each of the 12 independent chambers over a 100 kHz bandwidth with current resolution down to 5 pA for 100 ms measuring time. The design of the platform, its realization and experimental characterization are reported, with emphasis on the analysis of impact of input capacitance (i.e., microelectrode size) and microfluidic pump operation on current noise. Programmable sequences of successive injections of analytes (ferricyanide and dopamine) and rinsing buffer solution as well as the impedimetric continuous tracking for seven days of the proliferation of a colony of PC12 cells are successfully demonstrated.},
  language  = {en}
}