@article{LohmannStaceyBreuningeretal.2010, author = {Lohmann, Daniel and Stacey, Nicola and Breuninger, Holger and Jikumaru, Yusuke and M{\"u}ller, D{\"o}rte and Sicard, Adrien and Leyser, Ottoline and Yamaguchi, Shinjiro and Lenhard, Michael}, title = {SLOW MOTION is required for within-plant auxin homeostasis and normal timing of lateral organ initiation at the shoot meristem in Arabidopsis}, issn = {1040-4651}, year = {2010}, language = {en} } @article{FujikuraElsaesserBreuningeretal.2014, author = {Fujikura, Ushio and Elsaesser, Lore and Breuninger, Holger and Sanchez-Rodriguez, Clara and Ivakov, Alexander and Laux, Thomas and Findlay, Kim and Persson, Staffan and Lenhard, Michael}, title = {Atkinesin-13A modulates cell-wall synthesis and cell expansion in arabidopsis thaliana via the THESEUS1 pathway}, series = {PLoS Genetics : a peer-reviewed, open-access journal}, volume = {10}, journal = {PLoS Genetics : a peer-reviewed, open-access journal}, number = {9}, publisher = {PLoS}, address = {San Fransisco}, issn = {1553-7390}, doi = {10.1371/journal.pgen.1004627}, pages = {15}, year = {2014}, abstract = {Growth of plant organs relies on cell proliferation and expansion. While an increasingly detailed picture about the control of cell proliferation is emerging, our knowledge about the control of cell expansion remains more limited. We demonstrate the internal-motor kinesin AtKINESIN-13A (AtKIN13A) limits cell expansion and cell size in Arabidopsis thaliana, ion atkinl3a mutants forming larger petals with larger cells. The homolog, AtKINESIN-13B, also affects cell expansion and double mutants display growth, gametophytic and early embryonic defects, indicating a redundant role of he two genes. AtKIN13A is known to depolymerize microtubules and influence Golgi motility and distribution. Consistent his function, AtKIN13A interacts genetically with ANGUSTIFOLIA, encoding a regulator of Golgi dynamics. Reduced AtIGN13A activity alters cell wall structure as assessed by Fourier-transformed infrared-spectroscopy and triggers signalling he THESEUS1-dependent cell-wall integrity pathway, which in turn promotes the excess cell expansion in the atkinl3a mutant. Thus, our results indicate that the intracellular activity of AtKIN13A regulates cell expansion and wall architecture via THESEUS1, providing a compelling case of interplay between cell wall integrity sensing and expansion.}, language = {en} } @article{ErikssonStransfeldAdamskietal.2010, author = {Eriksson, Sven and Stransfeld, Lena and Adamski, Nikolai Maria and Breuninger, Holger and Lenhard, Michael}, title = {KLUH/CYP78A5-dependent growth signaling coordinates floral organ growth in Arabidopsis}, issn = {0960-9822}, year = {2010}, language = {en} } @article{CuongNguyenHuuKappelKelleretal.2016, author = {Cuong Nguyen Huu, and Kappel, Christian and Keller, Barbara and Sicard, Adrien and Takebayashi, Yumiko and Breuninger, Holger and Nowak, Michael D. and B{\"a}urle, Isabel and Himmelbach, Axel and Burkart, Michael and Ebbing-Lohaus, Thomas and Sakakibara, Hitoshi and Altschmied, Lothar and Conti, Elena and Lenhard, Michael}, title = {Presence versus absence of CYP734A50 underlies the style-length dimorphism in primroses}, series = {eLife}, volume = {5}, journal = {eLife}, publisher = {eLife Sciences Publications}, address = {Cambridge}, issn = {2050-084X}, doi = {10.7554/eLife.17956}, pages = {15}, year = {2016}, abstract = {Heterostyly is a wide-spread floral adaptation to promote outbreeding, yet its genetic basis and evolutionary origin remain poorly understood. In Primula (primroses), heterostyly is controlled by the S-locus supergene that determines the reciprocal arrangement of reproductive organs and incompatibility between the two morphs. However, the identities of the component genes remain unknown. Here, we identify the Primula CYP734A50 gene, encoding a putative brassinosteroid-degrading enzyme, as the G locus that determines the style-length dimorphism. CYP734A50 is only present on the short-styled S-morph haplotype, it is specifically expressed in S-morph styles, and its loss or inactivation leads to long styles. The gene arose by a duplication specific to the Primulaceae lineage and shows an accelerated rate of molecular evolution. Thus, our results provide a mechanistic explanation for the Primula style-length dimorphism and begin to shed light on the evolution of the S-locus as a prime model for a complex plant supergene.}, language = {en} } @article{BreuningerLenhard2010, author = {Breuninger, Holger and Lenhard, Michael}, title = {Control of tissue and organ growth in plants}, issn = {0070-2153}, year = {2010}, language = {en} } @misc{BreuningerLenhard2017, author = {Breuninger, Holger and Lenhard, Michael}, title = {Expression of the central growth regulator BIG BROTHER is regulated by multiple cis-elements}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-400971}, pages = {10}, year = {2017}, abstract = {Background Much of the organismal variation we observe in nature is due to differences in organ size. The observation that even closely related species can show large, stably inherited differences in organ size indicates a strong genetic component to the control of organ size. Despite recent progress in identifying factors controlling organ growth in plants, our overall understanding of this process remains limited, partly because the individual factors have not yet been connected into larger regulatory pathways or networks. To begin addressing this aim, we have studied the upstream regulation of expression of BIG BROTHER (BB), a central growth-control gene in Arabidopsis thaliana that prevents overgrowth of organs. Final organ size and BB expression levels are tightly correlated, implying the need for precise control of its expression. BB expression mirrors proliferative activity, yet the gene functions to limit proliferation, suggesting that it acts in an incoherent feedforward loop downstream of growth activators to prevent over-proliferation. Results To investigate the upstream regulation of BB we combined a promoter deletion analysis with a phylogenetic footprinting approach. We were able to narrow down important, highly conserved, cis-regulatory elements within the BB promoter. Promoter sequences of other Brassicaceae species were able to partially complement the A. thaliana bb-1 mutant, suggesting that at least within the Brassicaceae family the regulatory pathways are conserved. Conclusions This work underlines the complexity involved in precise quantitative control of gene expression and lays the foundation for identifying important upstream regulators that determine BB expression levels and thus final organ size.}, language = {en} } @article{BreuningerLenhard2012, author = {Breuninger, Holger and Lenhard, Michael}, title = {Expression of the central growth regulator BIG BROTHER is regulated by multiple cis-elements}, series = {BMC PLANT BIOLOGY}, volume = {12}, journal = {BMC PLANT BIOLOGY}, publisher = {BIOMED CENTRAL LTD}, address = {LONDON}, issn = {1471-2229}, doi = {10.1186/1471-2229-12-41}, pages = {10}, year = {2012}, abstract = {Background: Much of the organismal variation we observe in nature is due to differences in organ size. The observation that even closely related species can show large, stably inherited differences in organ size indicates a strong genetic component to the control of organ size. Despite recent progress in identifying factors controlling organ growth in plants, our overall understanding of this process remains limited, partly because the individual factors have not yet been connected into larger regulatory pathways or networks. To begin addressing this aim, we have studied the upstream regulation of expression of BIG BROTHER (BB), a central growth-control gene in Arabidopsis thaliana that prevents overgrowth of organs. Final organ size and BB expression levels are tightly correlated, implying the need for precise control of its expression. BB expression mirrors proliferative activity, yet the gene functions to limit proliferation, suggesting that it acts in an incoherent feedforward loop downstream of growth activators to prevent over-proliferation. Results: To investigate the upstream regulation of BB we combined a promoter deletion analysis with a phylogenetic footprinting approach. We were able to narrow down important, highly conserved, cis-regulatory elements within the BB promoter. Promoter sequences of other Brassicaceae species were able to partially complement the A. thaliana bb-1 mutant, suggesting that at least within the Brassicaceae family the regulatory pathways are conserved. Conclusions: This work underlines the complexity involved in precise quantitative control of gene expression and lays the foundation for identifying important upstream regulators that determine BB expression levels and thus final organ size.}, language = {en} }