@article{HessBartelRothetal.2012,
  author    = {Hess, Anne-Katrin and Bartel, Manuela and Roth, Karina and Messerschmidt, Katrin and Heilmann, Katja and Kenchington, Ellen and Micheel, Burkhard and Stuckas, Heiko},
  title     = {Expression of M6 and M7 lysin in Mytilus edulis is not restricted to sperm, but occurs also in oocytes and somatic tissue of males and females},
  series = {Molecular reproduction and development},
  volume    = {79},
  journal   = {Molecular reproduction and development},
  number    = {8},
  publisher = {Wiley-Blackwell},
  address   = {Hoboken},
  issn      = {1040-452X},
  doi       = {10.1002/mrd.22056},
  pages     = {517 -- 524},
  year      = {2012},
  abstract  = {Sperm proteins of marine sessile invertebrates have been extensively studied to understand the molecular basis of reproductive isolation. Apart from molecules such as bindin of sea urchins or lysin of abalone species, the acrosomal protein M7 lysin of Mytilus edulis has been analyzed. M7 lysin was found to be under positive selection, but mechanisms driving the evolution of this protein are not fully understood. To explore functional aspects, this study investigated the protein expression pattern of M7 and M6 lysin in gametes and somatic tissue of male and female M. edulis. The study employs a previously published monoclonal antibody (G26-AG8) to investigate M6 and M7 lysin protein expression, and explores expression of both genes. It is shown that these proteins and their encoding genes are expressed in gametes and somatic tissue of both sexes. This is in contrast to sea urchin bindin and abalone lysin, in which gene expression is strictly limited to males. Although future studies need to clarify the functional importance of both acrosomal proteins in male and female somatic tissue, new insights into the evolution of sperm proteins in marine sessile invertebrates are possible. This is because proteins with male-specific expression (bindin, lysin) might evolve differently than proteins with expression in both sexes (M6/M7 lysin), and the putative function of both proteins in females opens the possibility that the evolution of M6/M7 lysin is under sexual antagonistic selection, for example, mutations beneficial to the acrosomal function that are less beneficial the function in somatic tissue of females.Mol. Reprod. Dev. 79: 517-524, 2012.},
  language  = {en}
}
@article{BartelHartmannLehmannetal.2012,
  author    = {Bartel, Manuela and Hartmann, Stefanie and Lehmann, Karola and Postel, Kai and Quesada, Humberto and Philipp, Eva E. R. and Heilmann, Katja and Micheel, Burkhard and Stuckas, Heiko},
  title     = {Identification of sperm proteins as candidate biomarkers for the analysis of reproductive isolation in Mytilus: a case study for the enkurin locus},
  series = {Marine biology : international journal on life in oceans and coastal waters},
  volume    = {159},
  journal   = {Marine biology : international journal on life in oceans and coastal waters},
  number    = {10},
  publisher = {Springer},
  address   = {New York},
  issn      = {0025-3162},
  doi       = {10.1007/s00227-012-2005-7},
  pages     = {2195 -- 2207},
  year      = {2012},
  abstract  = {Sperm proteins of the marine sessile mussels of the Mytilus edulis species complex are models to investigate reproductive isolation and speciation. This study aimed at identifying sperm proteins and their corresponding genes. This was aided by the use of monoclonal antibodies that preferentially bind to yet unknown sperm molecules. By identifying their target molecules, this approach identified proteins with relevance to Mytilus sperm function. This procedure identified 16 proteins, for example, enkurin, laminin, porin and heat shock proteins. The potential use of these proteins as genetic markers to study reproductive isolation is exemplified by analysing the enkurin locus. Enkurin evolution is driven by purifying selection, the locus displays high levels of intraspecific variation and species-specific alleles group in distinct phylogenetic clusters. These findings characterize enkurin as informative candidate biomarker for analyses of clinal variation and differential introgression in hybrid zones, for example, to understand determinants of reproductive isolation in Baltic Mytilus populations.},
  language  = {en}
}