@article{WarsinkeBenkertScheller2000, author = {Warsinke, Axel and Benkert, Alexander and Scheller, Frieder W.}, title = {Electrochemical immunoassays}, year = {2000}, language = {en} } @article{VijgenboomVijgenboomTeppneretal.2001, author = {Vijgenboom, E. and Vijgenboom, E. and Teppner, A. W. J. W. and Makower, Alexander and Scheller, Frieder W. and Canters, Gerard W. and Wollenberger, Ursula}, title = {Determination of phenolic compounds using recombinant tyrosinanse from Streptomyces antibioticus}, year = {2001}, language = {en} } @article{TellerHalamekMakoweretal.2006, author = {Teller, C. and Halamek, Jan and Makower, Alexander and Fournier, Didier and Schulze, H. and Scheller, Frieder W.}, title = {A piezoelectric sensor with propidium as a recognition element for cholinesterases}, doi = {10.1016/j.snb.2005.02.053}, year = {2006}, abstract = {A piezoelectric biosensor has been developed on the basis of the reversible acetylcholinesterase (AChE) inhibitor propidium. The propidium cation was bound to a 11-mercaptoundecanoic acid monolayer on gold-coated quartz crystals. The immobilization was done via activation of carboxyl groups by 1,3-dicyclohexylcarbodiimide (DCC). Different types of cholinesterases (acetyl- and butyryl-ChE) from different origins were tested for their binding ability towards the immobilized propidium. Binding Studies were performed in a flow system, Furthermore, catalytically active and organophosphate-inhibited enzyme were compared re-aiding their binding capability. The binding constants were derived by using an one to one binding model and a refined model also including rebinding effects. It was shown that organophosphorylation of the active site hardly influences the affinity of AChE towards propidium. Furthermore the propidium-based biosensor provides equal sensitivity as compared with piezolelectric sensors with immobilized paraoxon- an active site ligand of AChE. (c) 2005 Elsevier B.V. All rights reserved}, language = {en} } @article{TanneJeoungPengetal.2015, author = {Tanne, Johannes and Jeoung, Jae-Hun and Peng, Lei and Yarman, Aysu and Dietzel, Birgit and Schulz, Burkhard and Schad, Daniel and Dobbek, Holger and Wollenberger, Ursula and Bier, Frank Fabian and Scheller, Frieder W.}, title = {Direct Electron Transfer and Bioelectrocatalysis by a Hexameric, Heme Protein at Nanostructured Electrodes}, series = {Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis}, volume = {27}, journal = {Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis}, number = {10}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1040-0397}, doi = {10.1002/elan.201500231}, pages = {2262 -- 2267}, year = {2015}, abstract = {A nanohybrid consisting of poly(3-aminobenzenesulfonic acid-co-aniline) and multiwalled carbon nanotubes [MWCNT-P(ABS-A)]) on a gold electrode was used to immobilize the hexameric tyrosine-coordinated heme protein (HTHP). The enzyme showed direct electron transfer between the heme group of the protein and the nanostructured surface. Desorption of the noncovalently bound heme from the protein could be excluded by control measurements with adsorbed hemin on aminohexanthiol-modified electrodes. The nanostructuring and the optimised charge characteristics resulted in a higher protein coverage as compared with MUA/MU modified electrodes. The adsorbed enzyme shows catalytic activity for the cathodic H2O2 reduction and oxidation of NADH.}, language = {en} } @article{TadjoungWaffoYesildagCasertaetal.2018, author = {Tadjoung Waffo, Armel Franklin and Yesildag, Cigdem and Caserta, Giorgio and Katz, Sagie and Zebger, Ingo and Lensen, Marga C. and Wollenberger, Ulla and Scheller, Frieder W. and Altintas, Zeynep}, title = {Fully electrochemical MIP sensor for artemisinin}, series = {Sensors and actuators : B, Chemical}, volume = {275}, journal = {Sensors and actuators : B, Chemical}, publisher = {Elsevier}, address = {Lausanne}, issn = {0925-4005}, doi = {10.1016/j.snb.2018.08.018}, pages = {163 -- 173}, year = {2018}, abstract = {This study aims to develop a rapid, sensitive and cost-effective biomimetic electrochemical sensor for artemisinin determination in plant extracts and for pharmacokinetic studies. A novel molecularly imprinted polymer (MIP)based electrochemical sensor was developed by electropolymerization of o-phenylenediamine (o-PD) in the presence of artemisinin on gold wire surface for sensitive detection of artemisinin. The experimental parameters, including selection of functional monomer, polymerization conditions, template extraction after polymerization, influence of pH and buffer were all optimized. Every step of imprinted film synthesis were evaluated by employing voltammetry techniques, surface-enhanced infrared absorption spectroscopy (SEIRAS) and atomic force microscopy (AFM). The specificity was further evaluated by investigating non-specific artemisinin binding on non-imprinted polymer (NIP) surfaces and an imprinting factor of 6.8 was achieved. The artemisinin imprinted polymers using o-PD as functional monomer have provided highly stable and effective binding cavities for artemisinin. Cross-reactivity studies with drug molecules showed that the MIPs are highly specific for artemisinin. The influence of matrix effect was further investigated both in artificial plant matrix and diluted human serum. The results revealed a high affinity of artemisinin-MIP with dissociation constant of 7.3 x 10(-9) M and with a detection limit of 0.01 mu M and 0.02 mu M in buffer and plant matrix, respectively.}, language = {en} } @article{SzeponikMoellerPfeifferetal.1997, author = {Szeponik, Jan and M{\"o}ller, B. and Pfeiffer, Dorothea and Lisdat, Fred and Wollenberger, Ursula and Makower, Alexander and Scheller, Frieder W.}, title = {Ultrasensitive bienzyme sensor for adrenaline}, year = {1997}, language = {en} } @article{StoellnerStoeckleinSchelleretal.2002, author = {St{\"o}llner, Daniela and St{\"o}cklein, Walter F. M. and Scheller, Frieder W. and Warsinke, Axel}, title = {Membrane-immobilized haptoglobin as affinity matrix for a hemoglobin-A1c-immunosensor}, year = {2002}, language = {en} } @article{StoeckleinWarsinkeScheller1997, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Scheller, Frieder W.}, title = {Organic solvent modified enzyme-liked immunoassay for the detection of triazine herbicides}, year = {1997}, language = {en} } @article{StoeckleinWarsinkeMicheeletal.1998, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Micheel, Burkhard and Kempter, Gerhard and H{\"o}hne, Wolfgang and Scheller, Frieder W.}, title = {Diphenylurea hapten sensing with a monoclonal antibody and its Fab fragment : kinetic and thermodynamic investigations}, year = {1998}, language = {en} } @article{StoeckleinWarsinkeMicheeletal.1998, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Micheel, Burkhard and H{\"o}hne, Wolfgang and Woller, Jochen and Kempter, Gerhard and Scheller, Frieder W.}, title = {Characterization of a monoclonal antibody and its Fab fragment against diphenylurea hapten with BIA}, isbn = {3-8154-3540-4}, year = {1998}, language = {en} } @article{StoeckleinWarsinkeMicheeletal.1997, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Micheel, Burkhard and H{\"o}hne, Wolfgang and Woller, Jochen and Kempter, Gerhard and Scheller, Frieder W.}, title = {Detection of diphenylurea derivatives with biospecific interaction analysis (BIA) : Kinetic investigations}, year = {1997}, language = {en} } @article{StoeckleinSchellerAbuknesha1995, author = {St{\"o}cklein, Walter F. M. and Scheller, Frieder W. and Abuknesha, Rhamadan}, title = {Effects of organic solvents on semicontinuous immunochemical detection of coumarin derivatives}, year = {1995}, language = {en} } @article{StoeckleinScheller1997, author = {St{\"o}cklein, Walter F. M. and Scheller, Frieder W.}, title = {Enzymes and antibodies in organic media : analytical applications}, year = {1997}, language = {en} } @article{StoeckleinScheller1996, author = {St{\"o}cklein, Walter F. M. and Scheller, Frieder W.}, title = {Laccase : a marker enzyme for solvent modified immunoassays}, year = {1996}, language = {en} } @article{StoeckleinRohdeScharteetal.2000, author = {St{\"o}cklein, Walter F. M. and Rohde, M. and Scharte, Gudrun and Behrsing, Olaf and Warsinke, Axel and Micheel, Burkhard and Scheller, Frieder W.}, title = {Sensitive detection of triazine and phenylurea pesticides in pure organic solvent by enzyme linked immunsorbent assay (ELISA): stabilities, solubilities and sensitives}, year = {2000}, language = {en} } @article{StoeckleinMakowerBieretal.1997, author = {St{\"o}cklein, Walter F. M. and Makower, Alexander and Bier, Frank Fabian and Scheller, Frieder W.}, title = {Enzyme sensors and enzyme amplifification systems}, year = {1997}, language = {en} } @article{StoeckleinBehrsingScharteetal.2000, author = {St{\"o}cklein, Walter F. M. and Behrsing, Olaf and Scharte, Gudrun and Micheel, Burkhard and Benkert, Alexander and Sch{\"o}ssler, W. and Warsinke, Axel and Scheller, Frieder W.}, title = {Enzyme kinetic assays with surface plasmon resonance (BIAcore) based on competition between enzyme and creatinine antibody}, year = {2000}, language = {en} } @article{StrefferKaatzBaueretal.1998, author = {Streffer, Katrin and Kaatz, Helvi and Bauer, Christian G. and Makower, Alexander and Schulmeister, Thomas and Scheller, Frieder W. and Peter, Martin G. and Wollenberger, Ursula}, title = {Application of a sensitive catechol detector for determination of tyrosinase inhibitors}, year = {1998}, language = {en} } @article{StojanovicErdossyKeltaietal.2017, author = {Stojanovic, Zorica and Erdossy, Julia and Keltai, Katalin and Scheller, Frieder W. and Gyurcsanyi, Robert E.}, title = {Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection}, series = {Analytica chimica acta : an international journal devoted to all branches of analytical chemistry}, volume = {977}, journal = {Analytica chimica acta : an international journal devoted to all branches of analytical chemistry}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0003-2670}, doi = {10.1016/j.aca.2017.04.043}, pages = {1 -- 9}, year = {2017}, abstract = {Molecularly imprinted polymers (MIPs) rendered selective solely by the imprinting with protein templates lacking of distinctive properties to facilitate strong target-MIP interaction are likely to exhibit medium to low template binding affinities. While this prohibits the use of such MIPs for applications requiring the assessment of very low template concentrations, their implementation for the quantification of high-abundance proteins seems to have a clear niche in the analytical practice. We investigated this opportunity by developing a polyscopoletin-based MIP nanofilm for the electrochemical determination of elevated human serum albumin (HSA) in urine. As reference for a low abundance protein ferritin-MIPs were also prepared by the same procedure. Under optimal conditions, the imprinted sensors gave a linear response to HSA in the concentration range of 20-100 mg/dm(3), and to ferritin in the range of 120-360 mg/dm(3). While as expected the obtained limit of detection was not sufficient to determine endogenous ferritin in plasma, the HSA-sensor was successfully employed to analyse urine samples of patients with albuminuria. The results suggest that MIP-based sensors may be applicable for quantifying high abundance proteins in a clinical setting. (c) 2017 Elsevier B.V. All rights reserved.}, language = {en} } @article{StoellnerSchellerWarsinke2002, author = {Stoellner, Daniela and Scheller, Frieder W. and Warsinke, Axel}, title = {Activation of cellulose membranes with 1,1{\"i}-carbonyldiimidazole or 1-cyano-4-4-dimethylaminopyridinium tetrafluoroborate as a basis for the development of immunosensors}, year = {2002}, language = {en} }