@article{JetzschmannTankJagerszkietal.2019,
  author    = {Jetzschmann, Katharina J. and Tank, Steffen and Jagerszki, Gyula and Gyurcsanyi, Robert E. and Wollenberger, Ulla and Scheller, Frieder W.},
  title     = {Bio-Electrosynthesis of Vectorially Imprinted Polymer Nanofilms for Cytochrome P450cam},
  series = {ChemElectroChem},
  volume    = {6},
  journal   = {ChemElectroChem},
  number    = {6},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {2196-0216},
  doi       = {10.1002/celc.201801851},
  pages     = {1818 -- 1823},
  year      = {2019},
  abstract  = {A new approach for synthesizing a vectorially imprinted polymer (VIP) is presented for the microbial cytochrome P450cam enzyme. A surface attached binding motif of a natural reaction partner of the target protein, putidaredoxin (Pdx), is the anchor to the underlying transducer. The 15 amino acid peptide anchor, which stems from the largest continuous amino acid chain within the binding site of Pdx was modified: (i) internal cysteines were replaced by serines to prevent disulfide bond formation; (ii) 2 ethylene glycol units were attached to the N-terminus as a spacer region; and (iii) an N-terminal cysteine was added to allow the immobilization on the gold electrode surface. Immobilization on GCE was achieved via an N-(1-pyrenyl)maleimide (NPM) cross-linker. In this way oriented immobilization of P450cam was accomplished by binding it to a peptide-modified gold or glassy carbon electrode (GCE) prior to the electrosynthesis of a polymer nanofilm around the immobilized target. This VIP nanofilm enabled reversible oriented docking of P450cam as it is indicated by the catalytic oxygen reduction via direct electron transfer between the enzyme and the underlying electrode. Catalysis of oxygen reduction by P450cam bound to the VIP-modified GCE was used to measure rebinding to the VIP. The mild coupling of an oxidoreductase with the electrode may be appropriate for realizing electrode-driven substrate conversion by instable P450 enzymes without the need of NADPH co-factor.},
  language  = {en}
}
@article{SchellerYarman2015,
  author    = {Scheller, Frieder W. and Yarman, Aysu},
  title     = {Bio vs. Mimetika in der Bioanalytik},
  series = {Biochemie und analytische Biochemie},
  volume    = {4},
  journal   = {Biochemie und analytische Biochemie},
  number    = {2},
  issn      = {2161-1009},
  pages     = {2},
  year      = {2015},
  abstract  = {Nat{\"u}rliche Evolution hat geschaffenBiopolymereauf der Basis von Aminos{\"a}uren undNukleotidezeigt hohe chemische Selektivit{\"a}t und katalytische Kraft. Die molekulare Erkennung durch Antik{\"o}rper und die katalytische Umwandlung der Substratmolek{\"u}le durch Enzyme findet in sogenannten Paratopen oder katalytischen Zentren des Makromolek{\"u}ls statt, die typischerweise 10-15 Aminos{\"a}uren umfassen. Die konzertierte Wechselwirkung zwischen den Reaktionspartnern f{\"u}hrt zu Affinit{\"a}ten bis zu nanomolaren Konzentrationen f{\"u}r die Antigenbindung und n{\"a}hert sich einer Million Ums{\"a}tze pro Sekunde anEnzym-katalysierte Reaktionen.},
  language  = {de}
}
@article{SchellerScheller1996,
  author    = {Scheller, Frieder W. and Scheller, A.},
  title     = {Bi-Enzymelektrode zur Messung von Sorbitol in pharmazeutischen Produkten},
  year      = {1996},
  language  = {de}
}
@article{BauerEremenkoKuehnetal.1998,
  author    = {Bauer, Christian G. and Eremenko, A. V. and K{\"u}hn, A. and K{\"u}rzinger, K. and Markower, Alexander and Scheller, Frieder W.},
  title     = {Automated amplifield flow immunoassay for cocaine},
  year      = {1998},
  language  = {en}
}
@article{OzcelikayKurbanogluYarmanetal.2020,
  author    = {Ozcelikay, Goksu and Kurbanoglu, Sevinc and Yarman, Aysu and Scheller, Frieder W. and Ozkan, Sibel A.},
  title     = {Au-Pt nanoparticles based molecularly imprinted nanosensor for electrochemical detection of the lipopeptide antibiotic drug Daptomycin},
  series = {Sensors and actuators : B, Chemical},
  volume    = {320},
  journal   = {Sensors and actuators : B, Chemical},
  publisher = {Elsevier Science},
  address   = {Amsterdam},
  issn      = {0925-4005},
  doi       = {10.1016/j.snb.2020.128285},
  pages     = {7},
  year      = {2020},
  abstract  = {In this work, a novel electrochemical molecularly imprinted polymer (MIP) sensor for the detection of the lipopeptide antibiotic Daptomycin (DAP) is presented which integrates gold decorated platinum nanoparticles (Au-Pt NPs) into the nanocomposite film. The sensor was prepared by electropolymerization of o-phenylenediamine (o-PD) in the presence of DAP using cyclic voltammetry. Cyclic voltammetry and differential pulse voltammetry were applied to follow the changes in the MIP-layer related to rebinding and removal of the target DAP by using the redox marker [Fe(CN)(6)](3-/4-). Under optimized operational conditions, the MIP/Au-Pt NPs/ GCE nanosensor exhibits a linear response in the range of 1-20 pM towards DAP. The limit of detection and limit of quantification were determined to be 0.161pM +/- 0.012 and 0.489pM +/- 0.012, respectively. The sensitivity towards the antibiotics Vancomycin and Erythromycin and the amino acids glycine and tryptophan was below 7 percent as compared with DAP. Moreover, the nanosensor was also successfully used for the detection of DAP in deproteinated human serum samples.},
  language  = {en}
}
@article{SchellerLisdatWollenberger2005,
  author    = {Scheller, Frieder W. and Lisdat, Fred and Wollenberger, Ursula},
  title     = {Application of electrically contacted enzymes for biosensors},
  isbn      = {3-527- 30690-0},
  year      = {2005},
  language  = {en}
}
@article{StrefferKaatzBaueretal.1998,
  author    = {Streffer, Katrin and Kaatz, Helvi and Bauer, Christian G. and Makower, Alexander and Schulmeister, Thomas and Scheller, Frieder W. and Peter, Martin G. and Wollenberger, Ursula},
  title     = {Application of a sensitive catechol detector for determination of tyrosinase inhibitors},
  year      = {1998},
  language  = {en}
}
@article{SchellerTiepnerWarsinke2004,
  author    = {Scheller, Frieder W. and Tiepner, K. and Warsinke, Axel},
  title     = {Anwendung von Biosensoren in der Lebensmittelanalytik},
  isbn      = {3-89947-120-2},
  year      = {2004},
  language  = {de}
}
@article{Scheller2002,
  author    = {Scheller, Frieder W.},
  title     = {Analytische Biochemie : Entwicklung von Biosensoren und Biochips},
  year      = {2002},
  language  = {de}
}
@article{SchellerBier2004,
  author    = {Scheller, Frieder W. and Bier, Frank Fabian},
  title     = {Analytical Biochemistry (Editorial)},
  year      = {2004},
  language  = {en}
}