@article{SchellerTiepnerWarsinke2004,
  author    = {Scheller, Frieder W. and Tiepner, K. and Warsinke, Axel},
  title     = {Anwendung von Biosensoren in der Lebensmittelanalytik},
  isbn      = {3-89947-120-2},
  year      = {2004},
  language  = {de}
}
@article{JeltschTewsBroseetal.2004,
  author    = {Jeltsch, Florian and Tews, J{\"o}rg and Brose, Ulrich and Grimm, Volker and Tielb{\"o}rger, Katja and Wichmann, Matthias and Schwager, Monika},
  title     = {Animal species diversity driven by habitat heterogeneity/diversity : the importance of keystone structures},
  year      = {2004},
  abstract  = {In a selected literature survey we reviewed studies on the habitat heterogeneity-animal species diversity relationship and evaluated whether there are uncertainties and biases in its empirical support. We reviewed 85 publications for the period 1960-2003. We screened each publication for terms that were used to define habitat heterogeneity, the animal species group and ecosystem studied, the definition of the structural variable, the measurement of vegetation structure and the temporal and spatial scale of the study. The majority of studies found a positive correlation between habitat heterogeneity/diversity and animal species diversity. However, empirical support for this relationship is drastically biased towards studies of vertebrates and habitats under anthropogenic influence. In this paper we show that ecological effects of habitat heterogeneity may vary considerably between species groups depending on whether structural attributes are perceived as heterogeneity or fragmentation. Possible effects may also vary relative to the structural variable measured. Based upon this, we introduce a classification framework that may be used for across-studies comparisons. Moreover, the effect of habitat heterogeneity for one species group may differ in relation to the spatial scale. In several studies, however, different species groups are closely linked to 'keystone structures' that determine animal species diversity by their presence. Detecting crucial keystone structures of the vegetation has profound implications for nature conservation and biodiversity management.},
  language  = {en}
}
@article{SchellerBier2004,
  author    = {Scheller, Frieder W. and Bier, Frank Fabian},
  title     = {Analytical Biochemistry (Editorial)},
  year      = {2004},
  language  = {en}
}
@article{ObelUsadelChooetal.2004,
  author    = {Obel, Nicolai and Usadel, Bj{\"o}rn and Choo, Tze Siang and Pauly, Markus},
  title     = {Analysing cell wall biosynthesis to study its role in biotic and abiotic stress reactions},
  isbn      = {3-00-011587-0},
  year      = {2004},
  language  = {en}
}
@article{Poeste2004,
  author    = {Poeste, Simon},
  title     = {Analyse von transgenen Kartoffelpflanzen mit ver{\"a}nderter cytosolischer Phosphorylase (Ph2) Aktivit{\"a}t},
  pages     = {102 S.},
  year      = {2004},
  language  = {de}
}
@article{LettauWarsinkeLaschewskyetal.2004,
  author    = {Lettau, Kristian and Warsinke, Axel and Laschewsky, Andr{\´e} and Mosbach, K. and Yilmaz, E. and Scheller, Frieder W.},
  title     = {An esterolytic imprinted polymer prepared via a silica-supported transition state analogue},
  year      = {2004},
  abstract  = {In this work we describe a new preparation method for an esterolytic imprinted polymer with catalytic sites on the surface. A template was prepared by immobilizing a transition state analogue (phosphoramidic acid derivative) of an esterolytic reaction within porous silica particles. Polymerization within the pores was carried out using 4- vinylimidazole as a functional monomer and divinylbenzene as a cross-linker. The polymer was released by dissolution of the silica support with hydrofluoric acid and catalytic properties were studied by incubation with three different 4- nitrophenylesters and spectrophotometric determination of the released 4-nitrophenol. For 4-nitrophenyl acetate an activity of 211 nmol min(-1) mg(-1) and a K-m value of 2.2 mmol L-1 was obtained},
  language  = {en}
}
@article{WannerAndersBischofetal.2004,
  author    = {Wanner, Manfred and Anders, Kenneth and Bischof, Ronny and Brozio, Fritz and Burkart, Bettina and Prochnow, Annette and Riedel, Heidi and Schneider, Dieter and Wiesener, Cornelia and Zulka, Klaus Peter and Zumkowski-Xylander, Helga and Xylander, Willi E. R.},
  title     = {Aktiver Truppen{\"u}bungsplatz Oberlausitz},
  isbn      = {3-540-22449-1},
  year      = {2004},
  language  = {de}
}
@article{GibbsReevesSunnaetal.2004,
  author    = {Gibbs, Moreland D. and Reeves, Rosalind A. and Sunna, Anwar and Bergquist, Peter L.},
  title     = {A yeast intron as a translational terminator in a plasmid shuttle vector},
  issn      = {1567-1356},
  year      = {2004},
  abstract  = {Plasmid shuttle vectors that contain both prokaryotic (Escherichia coli) and eukaryotic origins of replication are routinely used in molecular biology since E coli is generally the organism of choice for manipulation of recombinant DNA. Initial transformation of the shuttle vector into E coli allows production of microgram quantities of DNA suitable for transformation of low-transformationefficiency hosts. A shuttle/expression vector for the yeast Kluyveromyces lactis, pCWK1, allows recombinant protein fused to the killer toxin signal sequence to be secreted to the medium. The heterologous genes are transcribed under the control of the K lactis LAC4 promoter, which is tightly regulated in K lactis. However, in E coli the LAC4 promoter functions constitutively, and as a result, uncontrolled transcription and translation of genes that are toxic in E coli can result in cell death, and subsequent failure to recover intact E. coli transformants. We have constructed and tested a modified shuttle vector that contains a K lactis ribosomal intron that acts as a translational terminator in E coli, preventing or reducing the expression of recombinant proteins and avoiding toxicity. When transcribed in K lactis, the intron is spliced from the mRNA allowing the translation of intact full- length, active recombinant gene product. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved},
  language  = {en}
}
@article{BeissenhirtzSchellerLisdat2004,
  author    = {Beissenhirtz, Moritz Karl and Scheller, Frieder W. and Lisdat, Fred},
  title     = {A superoxide sensor based on a multilayer cytochrome c electrode},
  issn      = {0003-2700},
  year      = {2004},
  abstract  = {A novel multilayer cytochrome c electrode for the quantification of superoxide radical concentrations is introduced. The electrode consists of alternating layers of cytochrome c and poly(aniline(sulfonic acid)) on a gold wire electrode. The formation of multilayer structures was proven by SPR experiments. Assemblies with 2-15 protein layers showed electrochemical communication with the gold electrode. For every additional layer, a substantial increase in electrochemically active cytochrome c (cyt. c) was found. For electrodes of more than 10 layers, the increase was more than 1 order of magnitude as compared to monolayer electrode systems. Thermodynamic and kinetic parameters of the electrodes were characterized. The mechanism of electron transfer within the multilayer assembly was studied, with results suggesting a protein-protein electron-transfer model. Electrodes of 2-15 layers were applied to the in vitro quantification of enzymatically generated superoxide, showing superior sensitivity as compared to a monolayer-based sensor. An electrode with 6 cyt. c/PASA layers showed the highest sensitivity of the systems studied, giving an increase in sensitivity of half an order of magnitude versus the that of the monolayer electrode. The stability of the system was optimized using thermal treatment, resulting in no loss in sensor signal or protein loading after 10 successive measurements or 2 days of storage},
  language  = {en}
}
@article{KressJarrinThuroffetal.2004,
  author    = {Kress, H. and Jarrin, A. and Thuroff, E. and Saunders, R. and Weise, C. and Schmidt am Busch, Marcel and Knapp, E. W. and Wedde, M. and Vilcinskas, Andreas},
  title     = {A Kunitz type protease inhibitor related protein is synthesized in Drosophila prepupal salivary glands and released into the moulting fluid during pupation},
  issn      = {0965-1748},
  year      = {2004},
  abstract  = {From the Drosophila virilis late puff region 31C, we microcloned two neighbouring genes, Kil-1 and Kil-2, that encode putative Kunitz serine protease inhibitor like proteins. The Kil-1 gene is expressed exclusively in prepupal salivary glands. Using a size mutant of the KIL-1 protein and MALDI-TOF analysis, we demonstrate that during pupation this protein is released from the prepupal salivary glands into the pupation fluid covering the surface of the pupa. 3-D- structure predictions are consistent with the known crystal structure of the human Kunitz type protease inhibitor 2KNT. This is the first experimental proof for the extra-corporal presence of a distinct Drosophila prepupal salivary gland protein. Possible functions of KIL-1 in the context of the control of proteolytic activities in the pupation fluid are discussed. (C) 2004 Elsevier Ltd. All rights reserved},
  language  = {en}
}
@article{SchurrDeanMiltonetal.2004,
  author    = {Schurr, Frank Martin and Dean, W. R. J. and Milton, Sue J. and Jeltsch, Florian},
  title     = {A conceptual model linking demography of the shrub species Grewia flava to the dynamics of Kalahari savannas},
  year      = {2004},
  abstract  = {Environmental heterogeneity is a major determinant of plant population dynamics. In semi-arid Kalahari savannas, heterogeneity is created by savanna structure, i.e. by the spatial arrangement and temporal dynamics of woody plant and open grassland microsites. We formulate a conceptual model describing the effects of savanna dynamics on the population dynamics of the animal-dispersed shrub Grewia flava. From empirical results we derive model rules describing effects of savanna structure on several processes in Grewia's life cycle. By formulating the model, we summarise existing information on Grewia demography and identify gaps in this knowledge. Despite a number of such gaps, the model can be used to make certain quantitative predictions. As an example, we apply the model to investigate the role of seed dispersal in Grewia encroachment on rangelands. Model results show that cattle promote encroachment by depositing substantial numbers of seeds in open areas, where Grewia is otherwise dispersal-limited. Finally, we draw some general conclusions about Grewia's life history and population dynamics. Under natural conditions, concentrated seed deposition under woody plants appears to be a key process causing the observed association between Grewia and other woody plants. Furthermore, low rates of recruitment and high adult survival result in slow-motion dynamics of Grewia populations. As a consequence, Grewia populations interact with savanna dynamics on long temporal and short to intermediate spatial scales.},
  language  = {en}
}