@article{PuppeHoehnKaczoreketal.2016, author = {Puppe, Daniel and H{\"o}hn, Axel and Kaczorek, Danuta and Wanner, Manfred and Sommer, Michael}, title = {As time goes by-Spatiotemporal changes of biogenic Si pools in initial soils of an artificial catchment in NE Germany}, series = {Applied soil ecology : a section of agriculture, ecosystems \& environment}, volume = {105}, journal = {Applied soil ecology : a section of agriculture, ecosystems \& environment}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0929-1393}, doi = {10.1016/j.apsoil.2016.01.020}, pages = {9 -- 16}, year = {2016}, language = {en} } @article{Zimmermann2016, author = {Zimmermann, Andreas}, title = {Art. 116 Abs. 2 GG - ein verfassungsrechtliches Auslaufmodell}, series = {Die {\"O}ffentliche Verwaltung}, volume = {3}, journal = {Die {\"O}ffentliche Verwaltung}, publisher = {Kohlhammer}, address = {Stuttgart}, isbn = {0029-859X}, year = {2016}, abstract = {Zur Wiedergutmachung nationalsozialistischen Unrechts garantiert Art. 116 Abs. 2 GG Abk{\"o}mmlingen von in diskriminierender Weise ausgeb{\"u}rgerten Deutschen die deutsche Staatsangeh{\"o}rigkeit. Dadurch soll der Zustand wiederhergestellt werden, der ohne die Ausb{\"u}rgerung bestehen w{\"u}rde. Daher wird insoweit regelm{\"a}ßig auf das geltende Staatsangeh{\"o}rigkeitsrecht abgestellt. \S 4 Abs. 4 StAG hat im Jahr 2000 eine Beschr{\"a}nkung der Weitergabe der deutschen Staatsangeh{\"o}rigkeit f{\"u}r im Ausland geborene Kinder deutscher Staatsangeh{\"o}riger eingef{\"u}hrt, die selbst bereits im Ausland geboren wurden. Dadurch wird m{\"o}glicherweise der Anwendungsbereich des Art. 116 Abs. 2 GG dauerhaft signifikant eingeschr{\"a}nkt; unter Umst{\"a}nden wird die Norm gar obsolet. Vor diesem Hintergrund wird das Spannungsverh{\"a}ltnis zwischen der uneingeschr{\"a}nkten Anwendung des \S 4 Abs. 4 StAG mit dem verfassungsrechtlich verb{\"u}rgerten Recht auf Wiedereinb{\"u}rgerung von Kindern zu Unrecht ausgeb{\"u}rgerter Deutscher er{\"o}rtert.}, language = {de} } @article{HankeGogokhiaWuGerstneretal.2016, author = {Hanke-Gogokhia, Christin and Wu, Zhijian and Gerstner, Cecilia D. and Frederick, Jeanne M. and Zhang, Houbin and Baehr, Wolfgang}, title = {Arf-like Protein 3 (ARL3) Regulates Protein Trafficking and Ciliogenesis in Mouse Photoreceptors}, series = {The journal of biological chemistry}, volume = {291}, journal = {The journal of biological chemistry}, publisher = {American Society for Biochemistry and Molecular Biology}, address = {Bethesda}, issn = {0021-9258}, doi = {10.1074/jbc.M115.710954}, pages = {7142 -- 7155}, year = {2016}, abstract = {Arf-like protein 3 (ARL3) is a ubiquitous small GTPase expressed in ciliated cells of plants and animals. Germline deletion of Arl3 in mice causes multiorgan ciliopathy reminiscent of Bardet-Biedl or Joubert syndromes. As photoreceptors are elegantly compartmentalized and have cilia, we probed the function of ARL3 (ADP-ribosylation factor (Arf)-like 3 protein) by generating rod photoreceptor-specific (prefix (rod)) and retina-specific (prefix (ret)) Arl3 deletions. In predegenerate (rod)Arl3(-/-) mice, lipidated phototransduction proteins showed trafficking deficiencies, consistent with the role of ARL3 as a cargo displacement factor for lipid-binding proteins. By contrast, (ret)Arl3(-/-) rods and cones expressing Cre recombinase during embryonic development formed neither connecting cilia nor outer segments and degenerated rapidly. Absence of cilia infers participation of ARL3 in ciliogenesis and axoneme formation. Ciliogenesis was rescued, and degeneration was reversed in part by subretinal injection of adeno-associated virus particles expressing ARL3-EGFP. The conditional knock-out phenotypes permitted identification of two ARL3 functions, both in the GTP-bound form as follows: one as a regulator of intraflagellar transport participating in photoreceptor ciliogenesis and the other as a cargo displacement factor transporting lipidated protein to the outer segment. Surprisingly, a farnesylated inositol polyphosphate phosphatase only trafficked from the endoplasmic reticulum to the Golgi, thereby excluding it from a role in photoreceptor cilia physiology.}, language = {en} } @article{BrzezinkaAltmannCzesnicketal.2016, author = {Brzezinka, Krzysztof and Altmann, Simone and Czesnick, Hj{\"o}rdis and Nicolas, Philippe and Gorka, Michal and Benke, Eileen and Kabelitz, Tina and J{\"a}hne, Felix and Graf, Alexander and Kappel, Christian and B{\"a}urle, Isabel}, title = {Arabidopsis FORGETTER1 mediates stress-induced chromatin memory through nucleosome remodeling}, series = {eLife}, volume = {5}, journal = {eLife}, publisher = {eLife Sciences Publications}, address = {Cambridge}, issn = {2050-084X}, doi = {10.7554/eLife.17061}, pages = {23}, year = {2016}, abstract = {Plants as sessile organisms can adapt to environmental stress to mitigate its adverse effects. As part of such adaptation they maintain an active memory of heat stress for several days that promotes a more efficient response to recurring stress. We show that this heat stress memory requires the activity of the FORGETTER1 (FGT1) locus, with fgt1 mutants displaying reduced maintenance of heat-induced gene expression. FGT1 encodes the Arabidopsis thaliana orthologue of Strawberry notch (Sno), and the protein globally associates with the promoter regions of actively expressed genes in a heat-dependent fashion. FGT1 interacts with chromatin remodelers of the SWI/ SNF and ISWI families, which also display reduced heat stress memory. Genomic targets of the BRM remodeler overlap significantly with FGT1 targets. Accordingly, nucleosome dynamics at loci with altered maintenance of heat-induced expression are affected in fgt1. Together, our results suggest that by modulating nucleosome occupancy, FGT1 mediates stress-induced chromatin memory.}, language = {en} } @article{SunCernochVoelkeletal.2016, author = {Sun, Jing and Cernoch, Peter and V{\"o}lkel, Antje and Wei, Yuhan and Ruokolainen, Janne and Schlaad, Helmut}, title = {Aqueous Self-Assembly of a Protein-Mimetic Ampholytic Block Copolypeptide}, series = {Macromolecules : a publication of the American Chemical Society}, volume = {49}, journal = {Macromolecules : a publication of the American Chemical Society}, publisher = {American Chemical Society}, address = {Washington}, issn = {0024-9297}, doi = {10.1021/acs.macromol.6b00817}, pages = {5494 -- 5501}, year = {2016}, abstract = {This report describes the aggregation behavior of an ABC-type ampholytic block copolypeptide, poly(ethylene oxide)-block-poly(L-lysine)-block-poly(L-glutamate), in aqueous media in dependence of pH. Polypeptide secondary structures and self-assemblies are investigated by circular dichroism (CD), Fourier transform infrared (FT-IR) and NMR spectroscopy, zeta potential measurements, analytical ultracentrifugation (AUC), dynamic/static light scattering (DLS/SLS), and cryogenic transmission electron microscopy (cryoTEM). The polymer chains tend to form vesicles when the hydrophobic polypeptide helix is located at the chain end (acidic pH) and are existing as single chains when it is located in the center and flanked by the two hydrophilic segments (basic pH). Precipitation occurs in the intermediate pH range due to polyion complexation of the charged polypeptide segments.}, language = {en} } @article{GianniotisSchnoerrMolkenthinetal.2016, author = {Gianniotis, Nikolaos and Schnoerr, Christoph and Molkenthin, Christian and Bora, Sanjay Singh}, title = {Approximate variational inference based on a finite sample of Gaussian latent variables}, series = {Pattern Analysis \& Applications}, volume = {19}, journal = {Pattern Analysis \& Applications}, publisher = {Springer}, address = {New York}, issn = {1433-7541}, doi = {10.1007/s10044-015-0496-9}, pages = {475 -- 485}, year = {2016}, abstract = {Variational methods are employed in situations where exact Bayesian inference becomes intractable due to the difficulty in performing certain integrals. Typically, variational methods postulate a tractable posterior and formulate a lower bound on the desired integral to be approximated, e.g. marginal likelihood. The lower bound is then optimised with respect to its free parameters, the so-called variational parameters. However, this is not always possible as for certain integrals it is very challenging (or tedious) to come up with a suitable lower bound. Here, we propose a simple scheme that overcomes some of the awkward cases where the usual variational treatment becomes difficult. The scheme relies on a rewriting of the lower bound on the model log-likelihood. We demonstrate the proposed scheme on a number of synthetic and real examples, as well as on a real geophysical model for which the standard variational approaches are inapplicable.}, language = {en} } @article{GrossiReichardRuggiero2016, author = {Grossi, Giuseppe and Reichard, Christoph and Ruggiero, Pasquale}, title = {Appropriateness and Use of Performance Information in the Budgeting Process: Some Experiences from German and Italian Municipalities}, series = {Sports medicine}, volume = {39}, journal = {Sports medicine}, publisher = {Springer}, address = {Abingdon}, issn = {1530-9576}, doi = {10.1080/15309576.2015.1137770}, pages = {581 -- 606}, year = {2016}, abstract = {This article contributes to the debate on the use of performance information in the context of public sector performance management. Based on case studies, the authors analyze the appropriateness of the performance information provided in the newly established performance budgets of municipalities in Germany and Italy. They also examine the interest of politicians and senior managers in using such information for decision-making and monitoring within the municipal budget cycle. They find that the use of performance information is generally quite modest, and that the interest of different local actors varies to a great extent. Politicians are generally less interested in such information than top managers, particularly chief financial officers. The results are discussed by applying a theoretical framework based on institutional and legitimacy theories, and are compared with the literature on performance information use.}, language = {en} } @article{RoggenbuckBorghiSommaetal.2016, author = {Roggenbuck, Dirk and Borghi, Maria Orietta and Somma, Valentina and Buettner, Thomas and Schierack, Peter and Hanack, Katja and Grossi, Claudia and Bodio, Caterina and Macor, Paolo and von Landenberg, Philipp and Boccellato, Francesco and Mahler, Michael and Meroni, Pier Luigi}, title = {Antiphospholipid antibodies detected by line immunoassay differentiate among patients with antiphospholipid syndrome, with infections and asymptomatic carriers}, series = {IEEE transactions on geoscience and remote sensing}, volume = {18}, journal = {IEEE transactions on geoscience and remote sensing}, publisher = {BioMed Central}, address = {London}, issn = {1478-6354}, doi = {10.1186/s13075-016-1018-x}, pages = {14}, year = {2016}, abstract = {Background: Antiphospholipid antibodies (aPL) can be detected in asymptomatic carriers and infectious patients. The aim was to investigate whether a novel line immunoassay (LIA) differentiates between antiphospholipid syndrome (APS) and asymptomatic aPL+ carriers or patients with infectious diseases (infectious diseases controls (IDC)). Methods: Sixty-one patients with APS (56 primary, 22/56 with obstetric events only, and 5 secondary), 146 controls including 24 aPL+ asymptomatic carriers and 73 IDC were tested on a novel hydrophobic solid phase coated with cardiolipin (CL), phosphatic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, beta2-glycoprotein I (beta 2GPI), prothrombin, and annexin V. Samples were also tested by anti-CL and anti-beta 2GPI ELISAs and for lupus anticoagulant activity. Human monoclonal antibodies (humoAbs) against human beta 2GPI or PL alone were tested on the same LIA substrates in the absence or presence of human serum, purified human beta 2GPI or after CL-micelle absorption. Results: Comparison of LIA with the aPL-classification assays revealed good agreement for IgG/IgM a beta 2GPI and aCL. Anti-CL and anti-beta 2GPI IgG/IgM reactivity assessed by LIA was significantly higher in patients with APS versus healthy controls and IDCs, as detected by ELISA. IgG binding to CL and beta 2GPI in the LIA was significantly lower in aPL+ carriers and Venereal Disease Research Laboratory test (VDRL) + samples than in patients with APS. HumoAb against domain 1 recognized beta 2GPI bound to the LIA-matrix and in anionic phospholipid (PL) complexes. Absorption with CL micelles abolished the reactivity of a PL-specific humoAb but did not affect the binding of anti-beta 2GPI humoAbs. Conclusions: The LIA and ELISA have good agreement in detecting aPL in APS, but the LIA differentiates patients with APS from infectious patients and asymptomatic carriers, likely through the exposure of domain 1.}, language = {en} } @article{BarazaNeserJacksonetal.2016, author = {Baraza, Lilechi D. and Neser, Wekesa and Jackson, Korir Cheruiyot and Fredrick, Juma B. and Dennis, Ochieno and Wairimu, Kamau R. and Keya, Aggrey Osogo and Heydenreich, Matthias}, title = {Antimicrobial Coumarins from the Oyster Culinary-Medicinal Mushroom, Pleurotus ostreatus (Agaricomycetes), from Kenya}, series = {International journal of medicinal mushrooms}, volume = {18}, journal = {International journal of medicinal mushrooms}, publisher = {Begell House}, address = {Danbury}, issn = {1521-9437}, doi = {10.1615/IntJMedMushrooms.v18.i10.60}, pages = {905 -- 913}, year = {2016}, abstract = {Pleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. These have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. There are, however, few reports on the antimicrobial activities of different classes of purified compounds from P. ostreatus. This led to the current study, the objective of which was to chemically characterize the antibiotic activities of P. ()streams against selected human pathogenic bacteria and endophytic fungi. Chemical structures were determined using spectroscopic methods and by comparison with values of related structures reported in the literature. Pure compounds from P. ostreatus were tested in vitro against pathogenic bacteria (Staphylococcus aureus and Escherichia coli) and endophytic fungi (Pencillium digitatum and Fusarium prolferatum). A new compound, (E)-5,7-dimethoxy-6-(3-methylbuta-1,3-dienyl)-2H-chromen-2-one (5-methoxy-(E)-suberodiene) (compound 2), along with ergosterol (compound I.) and 5,7-dimethoxy-6-(3-methylbut-2-enyl)-2H-chromen-2-one (toddaculin; compound 3), were isolated from the fruiting bodies of P. ostreatus. The growth of S. aureus,E proliferatum, and P. digitatum colonies was inhibited in media containing compound 2, with minimum inhibitory concentrations closely comparable to those of conventional antibiotics.}, language = {en} } @article{KunnusJosefssonRajkovicetal.2016, author = {Kunnus, Kristjan and Josefsson, Ida and Rajkovic, Ivan and Schreck, Simon and Quevedo, Wilson and Beye, Martin and Gr{\"u}bel, Sebastian and Scholz, Mirko and Nordlund, Dennis and Zhang, Wenkai and Hartsock, Robert W. and Gaffney, Kelly J. and Schlotter, William F. and Turner, Joshua J. and Kennedy, Brian and Hennies, Franz and Techert, Simone and Wernet, Philippe and Odelius, Michael and F{\"o}hlisch, Alexander}, title = {Anti-Stokes resonant x-ray Raman scattering for atom specific and excited state selective dynamics}, series = {NEW JOURNAL OF PHYSICS}, volume = {18}, journal = {NEW JOURNAL OF PHYSICS}, publisher = {IOP Publ. Ltd.}, address = {Bristol}, issn = {1367-2630}, doi = {10.1088/1367-2630/18/10/103011}, pages = {9}, year = {2016}, abstract = {Ultrafast electronic and structural dynamics of matter govern rate and selectivity of chemical reactions, as well as phase transitions and efficient switching in functional materials. Since x-rays determine electronic and structural properties with elemental, chemical, orbital and magnetic selectivity, short pulse x-ray sources have become central enablers of ultrafast science. Despite of these strengths, ultrafast x-rays have been poor at picking up excited state moieties from the unexcited ones. With time-resolved anti-Stokes resonant x-ray Raman scattering (AS-RXRS) performed at the LCLS, and ab initio theory we establish background free excited state selectivity in addition to the elemental, chemical, orbital and magnetic selectivity of x-rays. This unparalleled selectivity extracts low concentration excited state species along the pathway of photo induced ligand exchange of Fe(CO)(5) in ethanol. Conceptually a full theoretical treatment of all accessible insights to excited state dynamics with AS-RXRS with transform-limited x-ray pulses is given-which will be covered experimentally by upcoming transform-limited x-ray sources.}, language = {en} }