@article{StoeckleinScheller1996, author = {St{\"o}cklein, Walter F. M. and Scheller, Frieder W.}, title = {Laccase : a marker enzyme for solvent modified immunoassays}, year = {1996}, language = {en} } @article{StoeckleinWarsinkeMicheeletal.1997, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Micheel, Burkhard and H{\"o}hne, Wolfgang and Woller, Jochen and Kempter, Gerhard and Scheller, Frieder W.}, title = {Detection of diphenylurea derivatives with biospecific interaction analysis (BIA) : Kinetic investigations}, year = {1997}, language = {en} } @article{StoeckleinScheller1997, author = {St{\"o}cklein, Walter F. M. and Scheller, Frieder W.}, title = {Enzymes and antibodies in organic media : analytical applications}, year = {1997}, language = {en} } @article{StoeckleinWarsinkeScheller1997, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Scheller, Frieder W.}, title = {Organic solvent modified enzyme-liked immunoassay for the detection of triazine herbicides}, year = {1997}, language = {en} } @article{KramerKeitelWinkleretal.1997, author = {Kramer, A. and Keitel, T. and Winkler, K. and St{\"o}cklein, Walter F. M. and H{\"u}hne, Wolfgang and Schneider-Mergener, Jens}, title = {Molecular basis for the binding promiscuity of an anti-P24 (HIV-1) monoclonal antibody}, year = {1997}, language = {en} } @article{StoeckleinMakowerBieretal.1997, author = {St{\"o}cklein, Walter F. M. and Makower, Alexander and Bier, Frank Fabian and Scheller, Frieder W.}, title = {Enzyme sensors and enzyme amplifification systems}, year = {1997}, language = {en} } @article{JinBernhardtStoeckleinetal.1998, author = {Jin, Wen and Bernhardt, Rita and St{\"o}cklein, Walter F. M. and Scheller, Frieder W.}, title = {Direct electron transfer of adrenodoxin-a [2Fe-2S] protein-- and its mutants on modified gold electrode}, year = {1998}, language = {en} } @article{StoeckleinWarsinkeMicheeletal.1998, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Micheel, Burkhard and Kempter, Gerhard and H{\"o}hne, Wolfgang and Scheller, Frieder W.}, title = {Diphenylurea hapten sensing with a monoclonal antibody and its Fab fragment : kinetic and thermodynamic investigations}, year = {1998}, language = {en} } @article{WollenbergerJinBernhardtetal.1998, author = {Wollenberger, Ursula and Jin, Wen and Bernhardt, Rita and Lehmann, Claudia and St{\"o}cklein, Walter F. M. and Brigelius-Floh{\´e}, Regina and Scheller, Frieder W.}, title = {Funktionalisierung von Elektroden f{\"u}r den direkten heterogenen Elektrotransfer}, year = {1998}, language = {de} } @article{StoeckleinWarsinkeMicheeletal.1998, author = {St{\"o}cklein, Walter F. M. and Warsinke, Axel and Micheel, Burkhard and H{\"o}hne, Wolfgang and Woller, Jochen and Kempter, Gerhard and Scheller, Frieder W.}, title = {Characterization of a monoclonal antibody and its Fab fragment against diphenylurea hapten with BIA}, isbn = {3-8154-3540-4}, year = {1998}, language = {en} } @article{StoeckleinRohdeScharteetal.2000, author = {St{\"o}cklein, Walter F. M. and Rohde, M. and Scharte, Gudrun and Behrsing, Olaf and Warsinke, Axel and Micheel, Burkhard and Scheller, Frieder W.}, title = {Sensitive detection of triazine and phenylurea pesticides in pure organic solvent by enzyme linked immunsorbent assay (ELISA): stabilities, solubilities and sensitives}, year = {2000}, language = {en} } @article{LisdatGeStoeckleinetal.2000, author = {Lisdat, Fred and Ge, Bixia and St{\"o}cklein, Walter F. M. and Scheller, Frieder W. and Meyer, T.}, title = {Electrochemical behaviour and nitric oxides interaction of immobilised cytochrome c from Rhodocyclus gelatinosus}, year = {2000}, language = {en} } @article{StoeckleinBehrsingScharteetal.2000, author = {St{\"o}cklein, Walter F. M. and Behrsing, Olaf and Scharte, Gudrun and Micheel, Burkhard and Benkert, Alexander and Sch{\"o}ssler, W. and Warsinke, Axel and Scheller, Frieder W.}, title = {Enzyme kinetic assays with surface plasmon resonance (BIAcore) based on competition between enzyme and creatinine antibody}, year = {2000}, language = {en} } @article{Stoecklein2000, author = {St{\"o}cklein, Walter F. M.}, title = {Biosensoren f{\"u}r die direkte vor-Ort {\"U}berwachung von Umwelt-Schadstoffen}, year = {2000}, language = {de} } @article{LisdatUtepbergenovHaseloffetal.2001, author = {Lisdat, Fred and Utepbergenov, D. and Haseloff, R. F. and Blasig, Ingolf E. and St{\"o}cklein, Walter F. M. and Scheller, Frieder W. and Brigelius-Floh{\´e}, Regina}, title = {An optical method for the detection of oxidative stress using protein-RNA interaction}, year = {2001}, language = {en} } @article{StoellnerStoeckleinSchelleretal.2002, author = {St{\"o}llner, Daniela and St{\"o}cklein, Walter F. M. and Scheller, Frieder W. and Warsinke, Axel}, title = {Membrane-immobilized haptoglobin as affinity matrix for a hemoglobin-A1c-immunosensor}, year = {2002}, language = {en} } @article{NiessnerBroekaertEinaxetal.2002, author = {Nießner, Reinhard and Broekaert, J. and Einax, J. W. and Scheller, Frieder W. and St{\"o}cklein, Walter F. M.}, title = {Trendbericht analytische Biochemie 2000/2001}, year = {2002}, language = {de} } @article{ChenStoeckleinSchelleretal.2003, author = {Chen, Jian and St{\"o}cklein, Walter F. M. and Scheller, Frieder W. and Wollenberger, Ursula}, title = {Electrochemical determination of human hemoglobin by using ferrocene carboxylic acid modified carbon powder microelectrode}, year = {2003}, language = {en} } @article{HimmelVanderVenStoeckleinetal.2003, author = {Himmel, Mirko and VanderVen, Peter F. M. and St{\"o}cklein, Walter F. M. and F{\"u}rst, Dieter Oswald}, title = {The limits of promiscuity : isoform-specific dimerization of filamins}, year = {2003}, language = {en} } @article{PieperFuerstKleuserStoeckleinetal.2004, author = {Pieper-F{\"u}rst, U. and Kleuser, U. and St{\"o}cklein, Walter F. M. and Warsinke, Axel and Scheller, Frieder W.}, title = {Detection of subicomolar concentrations of human matrix metalloproteinase-2 by an optical biosensor}, year = {2004}, abstract = {We describe in this paper the development of a one-step sandwich assay for the highly sensitive and fast detection of human matrix metalloproteinase (MMP)-2 (EC 3.4.24.24), using surface plasmon resonance (SPR). For the assay, two ligands were selected: monoclonal anti-MMP-2 antibody Ab-2 and the tissue inhibitor of metalloproteinases (TIMP)-2. They were chosen on the basis of (1) their affinities to MMP-2, (2) the efficiency of immobilization to the sensor chip, (3) the efficiency of adsorption to colloidal gold, and (4) the stability of these protein-coated gold particles. The assay included mixing of MMP-2 with antibody Ab-2 adsorbed to colloidal gold with a diameter of about 20 rim and injection into the flowcell of the SPR instrument containing immobilized TIMP-2. By using colloidal gold particles an amplification factor of 114 and a detection limit of 0.5 pM for MMP-2 were obtained. The precision of the assay was high even at low analyte concentrations, the standard deviation being 8.3\% for five determinations of 1 pM MMP- 2. No significant binding was observed with the structurally related MMP-9. The assay is far more sensitive and faster than commonly used methods for MMP-2 detection. As TIMP-bound MMP-2 is not detected by this method, the assay can be applied for measuring free MMP-2, reflecting the imbalance of free and inhibitor-bound enzyme in various pathological situations. (C) 2004 Elsevier Inc. All rights reserved}, language = {en} }