@article{PrueferWengerBieretal.2022,
  author    = {Pr{\"u}fer, Mareike and Wenger, Christian and Bier, Frank Fabian and Laux, Eva-Maria and H{\"o}lzel, Ralph},
  title     = {Activity of AC electrokinetically immobilized horseradish peroxidase},
  series = {Electrophoresis : microfluidics, nanoanalysis \& proteomics},
  journal   = {Electrophoresis : microfluidics, nanoanalysis \& proteomics},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0173-0835},
  doi       = {10.1002/elps.202200073},
  pages     = {1920 -- 1933},
  year      = {2022},
  abstract  = {Dielectrophoresis (DEP) is an AC electrokinetic effect mainly used to manipulate cells. Smaller particles, like virions, antibodies, enzymes, and even dye molecules can be immobilized by DEP as well. In principle, it was shown that enzymes are active after immobilization by DEP, but no quantification of the retained activity was reported so far. In this study, the activity of the enzyme horseradish peroxidase (HRP) is quantified after immobilization by DEP. For this, HRP is immobilized on regular arrays of titanium nitride ring electrodes of 500 nm diameter and 20 nm widths. The activity of HRP on the electrode chip is measured with a limit of detection of 60 fg HRP by observing the enzymatic turnover of Amplex Red and H2O2 to fluorescent resorufin by fluorescence microscopy. The initial activity of the permanently immobilized HRP equals up to 45\% of the activity that can be expected for an ideal monolayer of HRP molecules on all electrodes of the array. Localization of the immobilizate on the electrodes is accomplished by staining with the fluorescent product of the enzyme reaction. The high residual activity of enzymes after AC field induced immobilization shows the method's suitability for biosensing and research applications.},
  language  = {en}
}
@article{ReegStriglJeltsch2022,
  author    = {Reeg, Jette and Strigl, Lea and Jeltsch, Florian},
  title     = {Agricultural buffer zone thresholds to safeguard functional bee diversity},
  series = {Ecology and Evolution},
  volume    = {12},
  journal   = {Ecology and Evolution},
  edition   = {3},
  publisher = {Wiley Online Library},
  address   = {Hoboken, New Jersey, USA},
  issn      = {2045-7758},
  doi       = {10.1002/ece3.8748},
  pages     = {1 -- 17},
  year      = {2022},
  abstract  = {Wild bee species are important pollinators in agricultural landscapes. However, population decline was reported over the last decades and is still ongoing. While agricultural intensification is a major driver of the rapid loss of pollinating species, transition zones between arable fields and forest or grassland patches, i.e., agricultural buffer zones, are frequently mentioned as suitable mitigation measures to support wild bee populations and other pollinator species. Despite the reported general positive effect, it remains unclear which amount of buffer zones is needed to ensure a sustainable and permanent impact for enhancing bee diversity and abundance. To address this question at a pollinator community level, we implemented a process-based, spatially explicit simulation model of functional bee diversity dynamics in an agricultural landscape. More specifically, we introduced a variable amount of agricultural buffer zones (ABZs) at the transition of arable to grassland, or arable to forest patches to analyze the impact on bee functional diversity and functional richness. We focused our study on solitary bees in a typical agricultural area in the Northeast of Germany. Our results showed positive effects with at least 25\% of virtually implemented agricultural buffer zones. However, higher amounts of ABZs of at least 75\% should be considered to ensure a sufficient increase in Shannon diversity and decrease in quasi-extinction risks. These high amounts of ABZs represent effective conservation measures to safeguard the stability of pollination services provided by solitary bee species. As the model structure can be easily adapted to other mobile species in agricultural landscapes, our community approach offers the chance to compare the effectiveness of conservation measures also for other pollinator communities in future.},
  language  = {en}
}
@article{SchlaegelMaedlow2022,
  author    = {Schl{\"a}gel, Ulrike E. and M{\"a}dlow, Wolfgang},
  title     = {All-season space use by non-native resident Mandarin Ducks (Aix galericulata) in northeastern Germany},
  series = {Journal of ornithology / publ. by Deutsche Ornithologen-Gesellschaft},
  volume    = {163},
  journal   = {Journal of ornithology / publ. by Deutsche Ornithologen-Gesellschaft},
  number    = {1},
  publisher = {Springer},
  address   = {Berlin},
  issn      = {2193-7192},
  doi       = {10.1007/s10336-021-01932-7},
  pages     = {71 -- 82},
  year      = {2022},
  abstract  = {Patterns of space use are often subject to large temporal and individual-level variation, due to seasonality in behaviour and environmental conditions as well as age- or sex-specific needs. Especially in temperate regions, seasonality likely influences space use even in non-migratory birds. In waterfowl of the family Anatidae, however, few studies have analyzed space use of the same individuals across the full annual cycle. We used a resident population of Mandarin Ducks (Aix galericulata) in northeast Germany to study their year-round space use in relation to season, sex, and age. We marked 172 birds with colour rings and surveyed relevant water bodies for re-encounters for several years. As space-use patterns we derived home ranges from minimum convex polygons and the number of water bodies used by individual birds. Our analysis revealed that individuals shifted their space use between seasons, in particular extending their home ranges during the non-breeding season. Between years, in contrast, birds tended to show season-specific site fidelity. Sex differences were apparent during both breeding and non-breeding season, males consistently having larger home ranges and using slightly more water bodies. No difference was found between first-year and adult birds. Our study demonstrates that mark-resighting can provide valuable information about space use in species with suitable behaviour and readily accessible habitat. In such cases, it may be a valid alternative to more expensive GPS-tracking or short-term manual radio telemetry, particularly within citizen-science projects.},
  language  = {en}
}
@article{HussJuddKoperetal.2022,
  author    = {Huß, Sebastian and Judd, Rika Siedah and Koper, Kaan and Maeda, Hiroshi A. and Nikoloski, Zoran},
  title     = {An automated workflow that generates atom mappings for large-scale metabolic models and its application to Arabidopsis thaliana},
  series = {The plant journal},
  volume    = {111},
  journal   = {The plant journal},
  number    = {5},
  publisher = {Wiley-Blackwell},
  address   = {Oxford [u.a.]},
  issn      = {0960-7412},
  doi       = {10.1111/tpj.15903},
  pages     = {1486 -- 1500},
  year      = {2022},
  abstract  = {Quantification of reaction fluxes of metabolic networks can help us understand how the integration of different metabolic pathways determines cellular functions. Yet, intracellular fluxes cannot be measured directly but are estimated with metabolic flux analysis (MFA), which relies on the patterns of isotope labeling of metabolites in the network. The application of MFA also requires a stoichiometric model with atom mappings that are currently not available for the majority of large-scale metabolic network models, particularly of plants. While automated approaches such as the Reaction Decoder Toolkit (RDT) can produce atom mappings for individual reactions, tracing the flow of individual atoms of the entire reactions across a metabolic model remains challenging. Here we establish an automated workflow to obtain reliable atom mappings for large-scale metabolic models by refining the outcome of RDT, and apply the workflow to metabolic models of Arabidopsis thaliana. We demonstrate the accuracy of RDT through a comparative analysis with atom mappings from a large database of biochemical reactions, MetaCyc. We further show the utility of our automated workflow by simulating N-15 isotope enrichment and identifying nitrogen (N)-containing metabolites which show enrichment patterns that are informative for flux estimation in future N-15-MFA studies of A. thaliana. The automated workflow established in this study can be readily expanded to other species for which metabolic models have been established and the resulting atom mappings will facilitate MFA and graph-theoretic structural analyses with large-scale metabolic networks.},
  language  = {en}
}
@article{VencesKoehlerCrottinietal.2022,
  author    = {Vences, Miguel and K{\"o}hler, J{\"o}rn and Crottini, Angelica and Hofreiter, Michael and Hutter, Carl R. and du Preez, Louis and Preick, Michaela and Rakotoarison, Andolalao and Rancilhac, Lo{\"i}s and Raselimanana, Achille P. and Rosa, Gon{\c{c}}alo M. and Scherz, Mark D. and Glaw, Frank},
  title     = {An integrative taxonomic revision and redefinition of Gephyromantis (Laurentomantis) malagasius based on archival DNA analysis reveals four new mantellid frog species from Madagascar},
  series = {Vertebrate zoology},
  volume    = {72},
  journal   = {Vertebrate zoology},
  publisher = {Senckenberg Gesellschaft f{\"u}r Naturforschung},
  address   = {Frankfurt am Main},
  issn      = {1864-5755},
  doi       = {10.3897/vz.72.e78830},
  pages     = {271 -- 309},
  year      = {2022},
  abstract  = {The subgenus Laurentomantis in the genus Gephyromantis contains some of the least known amphibian species of Madagascar. The six currently valid nominal species are rainforest frogs known from few individuals, hampering a full understanding of the species diversity of the clade. We assembled data on specimens collected during field surveys over the past 30 years and integrated analysis of mitochondrial and nuclear-encoded genes of 88 individuals, a comprehensive bioacoustic analysis, and morphological comparisons to delimit a minimum of nine species-level lineages in the subgenus. To clarify the identity of the species Gephyromantis malagasius, we applied a target-enrichment approach to a sample of the 110 year old holotype of Microphryne malagasia Methuen and Hewitt, 1913 to assign this specimen to a lineage based on a mitochondrial DNA barcode. The holotype clustered unambiguously with specimens previously named G. ventrimaculatus. Consequently we propose to consider Trachymantis malagasia ventrimaculatus Angel, 1935 as a junior synonym of Gephyromantis malagasius. Due to this redefinition of G. malagasius, no scientific name is available for any of the four deep lineages of frogs previously subsumed under this name, all characterized by red color ventrally on the hindlimbs. These are here formally named as Gephyromantis fiharimpe sp. nov., G. matsilo sp. nov., G. oelkrugi sp. nov., and G. portonae sp. nov. The new species are distinguishable from each other by genetic divergences of >4\% uncorrected pairwise distance in a fragment of the 16S rRNA marker and a combination of morphological and bioacoustic characters. Gephyromantis fiharimpe and G. matsilo occur, respectively, at mid-elevations and lower elevations along a wide stretch of Madagascar's eastern rainforest band, while G. oelkrugi and G. portonae appear to be more range-restricted in parts of Madagascar's North East and Northern Central East regions. Open taxonomic questions surround G. horridus, to which we here assign specimens from Montagne d'Ambre and the type locality Nosy Be; and G. ranjomavo, which contains genetically divergent populations from Marojejy, Tsaratanana, and Ampotsidy.},
  language  = {en}
}
@article{LucenaPerezBazzicalupoPaijmansetal.2022,
  author    = {Lucena-Perez, Mar{\´i}a and Bazzicalupo, Enrico and Paijmans, Johanna and Kleinman-Ruiz, Daniel and Dal{\´e}n, Love and Hofreiter, Michael and Delibes, Miguel and Clavero, Miguel and Godoy, Jos{\´e} A.},
  title     = {Ancient genome provides insights into the history of Eurasian lynx in Iberia and Western Europe},
  series = {Quaternary science reviews : the international multidisciplinary research and review journal},
  volume    = {285},
  journal   = {Quaternary science reviews : the international multidisciplinary research and review journal},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {0277-3791},
  doi       = {10.1016/j.quascirev.2022.107518},
  pages     = {9},
  year      = {2022},
  abstract  = {The Eurasian lynx (Lynx lynx) is one of the most widely distributed felids in the world. However, most of its populations started to decline a few millennia ago. Historical declines have been especially severe in Europe, and particularly in Western Europe, from where the species disappeared in the last few centuries. Here, we analyze the genome of an Eurasian lynx inhabiting the Iberian Peninsula 2500 ya, to gain insights into the phylogeographic position and genetic status of this extinct population. Also, we contextualize previous ancient data in the light of new phylogeographic studies of the species. Our results suggest that the Iberian population is part of an extinct European lineage closely related to the current Carpathian-Baltic lineages. Also, this sample holds the lowest diversity reported for the species so far, and similar to that of the highly endangered Iberian lynx. A combination of historical factors, such as a founder effect while colonizing the peninsula, together with intensified human impacts during the Holocene in the Cantabrian strip, could have led to a genetic impoverishment of the population and precipitated its extinction. Mitogenomic lineages distribution in space and time support the long-term coexistence of several lineages of Eurasian lynx in Western Europe with fluctuating ranges. While mitochondrial sequences related to the lineages currently found in Balkans and Caucasus were predominant during the Pleistocene, those more closely related to the lineage currently distributed in Central Europe prevailed during the Holocene. The use of ancient genomics has proven to be a useful tool to understand the biogeographic pattern of the Eurasian lynx in the past.},
  language  = {en}
}
@article{IlicicWoodhouseKarstenetal.2022,
  author    = {Ilicic, Doris and Woodhouse, Jason Nicholas and Karsten, Ulf and Zimmermann, Jonas and Wichard, Thomas and Quartino, Maria Liliana and Campana, Gabriela Laura and Livenets, Alexandra and Van den Wyngaert, Silke and Grossart, Hans-Peter},
  title     = {Antarctic Glacial Meltwater Impacts the Diversity of Fungal Parasites Associated With Benthic Diatoms in Shallow Coastal Zones},
  series = {Frontiers in microbiology},
  journal   = {Frontiers in microbiology},
  number    = {13},
  publisher = {Frontiers Media},
  address   = {Lausanne},
  issn      = {1664-302X},
  doi       = {10.3389/fmicb.2022.805694},
  pages     = {12},
  year      = {2022},
  abstract  = {Aquatic ecosystems are frequently overlooked as fungal habitats, although there is increasing evidence that their diversity and ecological importance are greater than previously considered. Aquatic fungi are critical and abundant components of nutrient cycling and food web dynamics, e.g., exerting top-down control on phytoplankton communities and forming symbioses with many marine microorganisms. However, their relevance for microphytobenthic communities is almost unexplored. In the light of global warming, polar regions face extreme changes in abiotic factors with a severe impact on biodiversity and ecosystem functioning. Therefore, this study aimed to describe, for the first time, fungal diversity in Antarctic benthic habitats along the salinity gradient and to determine the co-occurrence of fungal parasites with their algal hosts, which were dominated by benthic diatoms. Our results reveal that Ascomycota and Chytridiomycota are the most abundant fungal taxa in these habitats. We show that also in Antarctic waters, salinity has a major impact on shaping not just fungal but rather the whole eukaryotic community composition, with a diversity of aquatic fungi increasing as salinity decreases. Moreover, we determined correlations between putative fungal parasites and potential benthic diatom hosts, highlighting the need for further systematic analysis of fungal diversity along with studies on taxonomy and ecological roles of Chytridiomycota.},
  language  = {en}
}
@article{BapolisiKielbBekiretal.2022,
  author    = {Bapolisi, Alain Murhimalika and Kielb, Patrycja and Bekir, Marek and Lehnen, Anne-Catherine and Radon, Christin and Laroque, Sophie and Wendler, Petra and M{\"u}ller-Werkmeister, Henrike and Hartlieb, Matthias},
  title     = {Antimicrobial polymers of linear and bottlebrush architecture},
  series = {Macromolecular rapid communications : publishing the newsletters of the European Polymer Federation},
  volume    = {43},
  journal   = {Macromolecular rapid communications : publishing the newsletters of the European Polymer Federation},
  number    = {19},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1521-3927},
  doi       = {10.1002/marc.202200288},
  pages     = {14},
  year      = {2022},
  abstract  = {Polymeric antimicrobial peptide mimics are a promising alternative for the future management of the daunting problems associated with antimicrobial resistance. However, the development of successful antimicrobial polymers (APs) requires careful control of factors such as amphiphilic balance, molecular weight, dispersity, sequence, and architecture. While most of the earlier developed APs focus on random linear copolymers, the development of APs with advanced architectures proves to be more potent. It is recently developed multivalent bottlebrush APs with improved antibacterial and hemocompatibility profiles, outperforming their linear counterparts. Understanding the rationale behind the outstanding biological activity of these newly developed antimicrobials is vital to further improving their performance. This work investigates the physicochemical properties governing the differences in activity between linear and bottlebrush architectures using various spectroscopic and microscopic techniques. Linear copolymers are more solvated, thermo-responsive, and possess facial amphiphilicity resulting in random aggregations when interacting with liposomes mimicking Escheria coli membranes. The bottlebrush copolymers adopt a more stable secondary conformation in aqueous solution in comparison to linear copolymers, conferring rapid and more specific binding mechanism to membranes. The advantageous physicochemical properties of the bottlebrush topology seem to be a determinant factor in the activity of these promising APs.},
  language  = {en}
}
@article{GhafarianWielandLuettschwageretal.2022,
  author    = {Ghafarian, Fatemeh and Wieland, Ralf and L{\"u}ttschwager, Dietmar and Nendel, Claas},
  title     = {Application of extreme gradient boosting and Shapley Additive explanations to predict temperature regimes inside forests from standard open-field meteorological data},
  series = {Environmental modelling \& software with environment data news},
  volume    = {156},
  journal   = {Environmental modelling \& software with environment data news},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {1364-8152},
  doi       = {10.1016/j.envsoft.2022.105466},
  pages     = {11},
  year      = {2022},
  abstract  = {Forest microclimate can buffer biotic responses to summer heat waves, which are expected to become more extreme under climate warming. Prediction of forest microclimate is limited because meteorological observation standards seldom include situations inside forests. We use eXtreme Gradient Boosting - a Machine Learning technique - to predict the microclimate of forest sites in Brandenburg, Germany, using seasonal data comprising weather features. The analysis was amended by applying a SHapley Additive explanation to show the interaction effect of variables and individualised feature attributions. We evaluate model performance in comparison to artificial neural networks, random forest, support vector machine, and multi-linear regression. After implementing a feature selection, an ensemble approach was applied to combine individual models for each forest and improve robustness over a given single prediction model. The resulting model can be applied to translate climate change scenarios into temperatures inside forests to assess temperature-related ecosystem services provided by forests.},
  language  = {en}
}
@article{VatovaRubinGrossartetal.2022,
  author    = {Vatova, Mariyana and Rubin, Conrad and Grossart, Hans-Peter and Goncalves, Susana C. and Schmidt, Susanne I. and Jarić, Ivan},
  title     = {Aquatic fungi: largely neglected targets for conservation},
  series = {Frontiers in ecology and the environment},
  volume    = {20},
  journal   = {Frontiers in ecology and the environment},
  number    = {4},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {1540-9295},
  doi       = {10.1002/fee.2495},
  pages     = {207 -- 209},
  year      = {2022},
  language  = {en}
}
@article{RadosavljevicLantuitKnoblauchetal.2022,
  author    = {Radosavljevic, Boris and Lantuit, Hugues and Knoblauch, Christian and Couture, Nicole and Herzschuh, Ulrike and Fritz, Michael},
  title     = {Arctic nearshore sediment dynamics - an example from Herschel Island - Qikiqtaruk, Canada},
  series = {Journal of marine science and engineering},
  volume    = {10},
  journal   = {Journal of marine science and engineering},
  number    = {11},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2077-1312},
  doi       = {10.3390/jmse10111589},
  pages     = {18},
  year      = {2022},
  abstract  = {Increasing arctic coastal erosion rates imply a greater release of sediments and organic matter into the coastal zone. With 213 sediment samples taken around Herschel Island-Qikiqtaruk, Canadian Beaufort Sea, we aimed to gain new insights on sediment dynamics and geochemical properties of a shallow arctic nearshore zone. Spatial characteristics of nearshore sediment texture (moderately to poorly sorted silt) are dictated by hydrodynamic processes, but ice-related processes also play a role. We determined organic matter (OM) distribution and inferred the origin and quality of organic carbon by C/N ratios and stable carbon isotopes delta C-13. The carbon content was higher offshore and in sheltered areas (mean: 1.0 wt.\%., S.D.: 0.9) and the C/N ratios also showed a similar spatial pattern (mean: 11.1, S.D.: 3.1), while the delta C-13 (mean: -26.4 parts per thousand VPDB, S.D.: 0.4) distribution was more complex. We compared the geochemical parameters of our study with terrestrial and marine samples from other studies using a bootstrap approach. Sediments of the current study contained 6.5 times and 1.8 times less total organic carbon than undisturbed and disturbed terrestrial sediments, respectively. Therefore, degradation of OM and separation of carbon pools take place on land and continue in the nearshore zone, where OM is leached, mineralized, or transported beyond the study area.},
  language  = {en}
}
@article{JesusSchmidtFickeletal.2022,
  author    = {Jesus, Sonia A. and Schmidt, Anke and Fickel, J{\"o}rns and Doherr, Marcus G. and Boonprasert, Khajohnpat and Thitaram, Chatchote and Sariya, Ladawan and Ratanakron, Parntep and Hildebrandt, Thomas Bernd},
  title     = {Assessing coagulation parameters in healthy Asian Elephants (Elephas maximus) from European and thai populations},
  series = {Animals},
  volume    = {12},
  journal   = {Animals},
  number    = {3},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2076-2615},
  doi       = {10.3390/ani12030361},
  pages     = {16},
  year      = {2022},
  abstract  = {Simple Summary Asian elephants (Elephas maximus) are considered endangered and their population is in continuous decline. Understanding their social interactions, health, and welfare status has been a topic of intense research in recent decades. Coagulation assessments have been underutilized in wildlife but can give valuable information on individual health. This study aims to increase the knowledge of the coagulation status in healthy Asian elephants from different backgrounds and age groups, using a fast point-of-care analyzer. This tool can be further used in either routine health check-ups performed by caretakers or in a clinical emergency, such as in cases of elephant endotheliotropic herpesvirus hemorrhagic disease outbreaks. We have also investigated the presence of genomic mutations in one coagulation factor-factor VII-where a disorder was previously reported in an Asian elephant. Hereby, we report new reference values for coagulation parameters, such as coagulation times and fibrinogen concentration of Asian elephants assessed in Thailand and in Europe, as well as several single point mutations found in the exons of Elephas maximus coagulation F7 gene. We found the point-of-care analyzer used in this study to be very practical and user friendly for a zoo and field environment and hope that this project will incentivize further coagulation studies in Asian elephants and in other wildlife species. The Asian elephant population is continuously declining due to several extrinsic reasons in their range countries, but also due to diseases in captive populations worldwide. One of these diseases, the elephant endotheliotropic herpesvirus (EEHV) hemorrhagic disease, is very impactful because it particularly affects Asian elephant calves. It is commonly fatal and presents as an acute and generalized hemorrhagic syndrome. Therefore, having reference values of coagulation parameters, and obtaining such values for diseased animals in a very short time, is of great importance. We analyzed prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen concentrations using a portable and fast point-of-care analyzer (VetScan Pro) in 127 Asian elephants from Thai camps and European captive herds. We found significantly different PT and aPTT coagulation times between elephants from the two regions, as well as clear differences in fibrinogen concentration. Nevertheless, these alterations were not expected to have biological or clinical implications. We have also sequenced the coagulation factor VII gene of 141 animals to assess the presence of a previously reported hereditary coagulation disorder in Asian elephants and to investigate the presence of other mutations. We did not find the previously reported mutation in our study population. Instead, we discovered the presence of several new single nucleotide polymorphisms, two of them being considered as deleterious by effect prediction software.},
  language  = {en}
}
@article{PawlakNoetzelDragoetal.2022,
  author    = {Pawlak, Julia and Noetzel, Dominique Christian and Drago, Claudia and Weithoff, Guntram},
  title     = {Assessing the toxicity of polystyrene beads and silica particles on the microconsumer Brachionus calyciflorus at different timescales},
  series = {Frontiers in Environmental Science},
  journal   = {Frontiers in Environmental Science},
  publisher = {Frontiers},
  address   = {Lausanne, Schweiz},
  issn      = {2296-665X},
  doi       = {10.3389/fenvs.2022.955425},
  pages     = {1 -- 11},
  year      = {2022},
  abstract  = {Environmental pollution by microplastics has become a severe problem in terrestrial and aquatic ecosystems and, according to actual prognoses, problems will further increase in the future. Therefore, assessing and quantifying the risk for the biota is crucial. Standardized short-term toxicological procedures as well as methods quantifying potential toxic effects over the whole life span of an animal are required. We studied the effect of the microplastic polystyrene on the survival and reproduction of a common freshwater invertebrate, the rotifer Brachionus calyciflorus, at different timescales. We used pristine polystyrene spheres of 1, 3, and 6 µm diameter and fed them to the animals together with food algae in different ratios ranging from 0 to 50\% nonfood particles. As a particle control, we used silica to distinguish between a pure particle effect and a plastic effect. After 24 h, no toxic effect was found, neither with polystyrene nor with silica. After 96 h, a toxic effect was detectable for both particle types. The size of the particles played a negligible role. Studying the long-term effect by using life table experiments, we found a reduced reproduction when the animals were fed with 3 µm spheres together with similar-sized food algae. We conclude that the fitness reduction is mainly driven by the dilution of food by the nonfood particles rather than by a direct toxic effect.},
  language  = {en}
}
@article{EsmaeilishirazifardUsherTrimetal.2022,
  author    = {Esmaeilishirazifard, Elham and Usher, Louise and Trim, Carol and Denise, Hubert and Sangal, Vartul and Tyson, Gregory H. and Barlow, Axel and Redway, Keith F. and Taylor, John D. and Kremyda-Vlachou, Myrto and Davies, Sam and Loftus, Teresa D. and Lock, Mikaella M. G. and Wright, Kstir and Dalby, Andrew and Snyder, Lori A. S. and Wuster, Wolfgang and Trim, Steve and Moschos, Sterghios A.},
  title     = {Bacterial adaptation to venom in snakes and arachnida},
  series = {Microbiology spectrum},
  volume    = {10},
  journal   = {Microbiology spectrum},
  number    = {3},
  publisher = {American Society for Microbiology},
  address   = {Birmingham, Ala.},
  issn      = {2165-0497},
  doi       = {10.1128/spectrum.02408-21},
  pages     = {16},
  year      = {2022},
  abstract  = {Notwithstanding their 3 to 5\% mortality, the 2.7 million envenomation-related injuries occurring annually-predominantly across Africa, Asia, and Latin America-are also major causes of morbidity. Venom toxin-damaged tissue will develop infections in some 75\% of envenomation victims, with E. faecalis being a common culprit of disease; however, such infections are generally considered to be independent of envenomation. Animal venoms are considered sterile sources of antimicrobial compounds with strong membrane-disrupting activity against multidrug-resistant bacteria. However, venomous bite wound infections are common in developing nations. Investigating the envenomation organ and venom microbiota of five snake and two spider species, we observed venom community structures that depend on the host venomous animal species and evidenced recovery of viable microorganisms from black-necked spitting cobra (Naja nigricollis) and Indian ornamental tarantula (Poecilotheria regalis) venoms. Among the bacterial isolates recovered from N. nigricollis, we identified two venom-resistant, novel sequence types of Enterococcus faecalis whose genomes feature 16 virulence genes, indicating infectious potential, and 45 additional genes, nearly half of which improve bacterial membrane integrity. Our findings challenge the dogma of venom sterility and indicate an increased primary infection risk in the clinical management of venomous animal bite wounds. IMPORTANCE Notwithstanding their 3 to 5\% mortality, the 2.7 million envenomation-related injuries occurring annually-predominantly across Africa, Asia, and Latin America-are also major causes of morbidity. Venom toxin-damaged tissue will develop infections in some 75\% of envenomation victims, with E. faecalis being a common culprit of disease; however, such infections are generally considered to be independent of envenomation. Here, we provide evidence on venom microbiota across snakes and arachnida and report on the convergent evolution mechanisms that can facilitate adaptation to black-necked cobra venom in two independent E. faecalis strains, easily misidentified by biochemical diagnostics. Therefore, since inoculation with viable and virulence gene-harboring bacteria can occur during envenomation, acute infection risk management following envenomation is warranted, particularly for immunocompromised and malnourished victims in resource-limited settings. These results shed light on how bacteria evolve for survival in one of the most extreme environments on Earth and how venomous bites must be also treated for infections.},
  language  = {en}
}
@article{IlicicGrossart2022,
  author    = {Ilicic, Doris and Grossart, Hans-Peter},
  title     = {Basal parasitic fungi in marine food webs-a mystery yet to unravel},
  series = {Journal of Fungi},
  volume    = {8},
  journal   = {Journal of Fungi},
  number    = {2},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2309-608X},
  doi       = {10.3390/jof8020114},
  pages     = {16},
  year      = {2022},
  abstract  = {Although aquatic and parasitic fungi have been well known for more than 100 years, they have only recently received increased awareness due to their key roles in microbial food webs and biogeochemical cycles. There is growing evidence indicating that fungi inhabit a wide range of marine habitats, from the deep sea all the way to surface waters, and recent advances in molecular tools, in particular metagenome approaches, reveal that their diversity is much greater and their ecological roles more important than previously considered. Parasitism constitutes one of the most widespread ecological interactions in nature, occurring in almost all environments. Despite that, the diversity of fungal parasites, their ecological functions, and, in particular their interactions with other microorganisms remain largely speculative, unexplored and are often missing from current theoretical concepts in marine ecology and biogeochemistry. In this review, we summarize and discuss recent research avenues on parasitic fungi and their ecological potential in marine ecosystems, e.g., the fungal shunt, and emphasize the need for further research.},
  language  = {en}
}
@article{ParrySchlaegelTiedemannetal.2022,
  author    = {Parry, Victor and Schl{\"a}gel, Ulrike E. and Tiedemann, Ralph and Weithoff, Guntram},
  title     = {Behavioural Responses of Defended and Undefended Prey to Their Predator},
  series = {Biology},
  volume    = {11},
  journal   = {Biology},
  number    = {8},
  publisher = {MDPI},
  address   = {Basel, Schweiz},
  issn      = {2079-7737},
  doi       = {10.3390/biology11081217},
  pages     = {14},
  year      = {2022},
  abstract  = {Predation is a strong species interaction causing severe harm or death to prey. Thus, prey species have evolved various defence strategies to minimize predation risk, which may be immediate (e.g., a change in behaviour) or transgenerational (morphological defence structures). We studied the behaviour of two strains of a rotiferan prey (Brachionus calyciflorus) that differ in their ability to develop morphological defences in response to their predator Asplanchna brightwellii. Using video analysis, we tested: (a) if two strains differ in their response to predator presence and predator cues when both are undefended; (b) whether defended individuals respond to live predators or their cues; and (c) if the morphological defence (large spines) per se has an effect on the swimming behaviour. We found a clear increase in swimming speed for both undefended strains in predator presence. However, the defended specimens responded neither to the predator presence nor to their cues, showing that they behave indifferently to their predator when they are defended. We did not detect an effect of the spines on the swimming behaviour. Our study demonstrates a complex plastic behaviour of the prey, not only in the presence of their predator, but also with respect to their defence status.},
  language  = {en}
}
@article{ParrySchlaegelTiedemannetal.2022,
  author    = {Parry, Victor and Schl{\"a}gel, Ulrike E. and Tiedemann, Ralph and Weithoff, Guntram},
  title     = {Behavioural responses of defended and undefended prey to their predator},
  series = {Biology : open access journal},
  volume    = {11},
  journal   = {Biology : open access journal},
  number    = {8},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2079-7737},
  doi       = {10.3390/biology11081217},
  pages     = {14},
  year      = {2022},
  abstract  = {Many animals that have to cope with predation have evolved mechanisms to reduce their predation risk. One of these mechanisms is change in morphology, for example, the development of spines. These spines are induced, when mothers receive chemical signals of a predator (kairomones) and their daughters are then equipped with defensive spines. We studied the behaviour of a prey and its predator when the prey is either defended or undefended. We used common aquatic micro-invertebrates, the rotifers Brachionus calyciflorus (prey) and Asplanchna brightwellii (predator) as experimental animals. We found that undefended prey increased its swimming speed in the presence of the predator. The striking result was that the defended prey did not respond to the predator's presence. This suggests that defended prey has a different response behaviour to a predator than undefended conspecifics. Our study provides further insights into complex zooplankton predator-prey interactions. Predation is a strong species interaction causing severe harm or death to prey. Thus, prey species have evolved various defence strategies to minimize predation risk, which may be immediate (e.g., a change in behaviour) or transgenerational (morphological defence structures). We studied the behaviour of two strains of a rotiferan prey (Brachionus calyciflorus) that differ in their ability to develop morphological defences in response to their predator Asplanchna brightwellii. Using video analysis, we tested: (a) if two strains differ in their response to predator presence and predator cues when both are undefended; (b) whether defended individuals respond to live predators or their cues; and (c) if the morphological defence (large spines) per se has an effect on the swimming behaviour. We found a clear increase in swimming speed for both undefended strains in predator presence. However, the defended specimens responded neither to the predator presence nor to their cues, showing that they behave indifferently to their predator when they are defended. We did not detect an effect of the spines on the swimming behaviour. Our study demonstrates a complex plastic behaviour of the prey, not only in the presence of their predator, but also with respect to their defence status.},
  language  = {en}
}
@article{EscalanteDominguezGomezRuizetal.2022,
  author    = {Escalante, Ignacio and Dominguez, Marisol and Gomez-Ruiz, Daisy Alejandra and Machado, Glauco},
  title     = {Benefits and costs of mixed-species aggregations in Harvestmen (Arachnida: Opiliones)},
  series = {Frontiers in ecology and evolution},
  volume    = {9},
  journal   = {Frontiers in ecology and evolution},
  publisher = {Frontiers Media},
  address   = {Lausanne},
  issn      = {2296-701X},
  doi       = {10.3389/fevo.2021.766323},
  pages     = {24},
  year      = {2022},
  abstract  = {Many animals form aggregations with individuals of the same species (single-species aggregations, SSA). Less frequently, individuals may also aggregate with individuals of other species (mixed-species aggregations, MSA). Although the benefits and costs of SSA have been intensively studied, the same is not true for MSA. Here, we first review the cases of MSA in harvestmen, an arachnid order in which the records of MSA are more frequent than other arthropod orders. We then propose several benefits and costs of MSA in harvestmen, and contrast them with those of SSA. Second, using field-gathered data we describe gregariousness in seven species of Prionostemma harvestmen from Costa Rica. These species form MSA, but individuals are also found solitarily or in SSA. We tested one possible benefit and one possible cost of gregariousness in Prionostemma harvestmen. Regarding the benefit, we hypothesized that individuals missing legs would be more exposed to predation than eight-legged individuals and thus they should be found preferentially in aggregations, where they would be more protected from predators. Our data, however, do not support this hypothesis. Regarding the cost, we hypothesized that gregariousness increases the chances of parasitism. We found no support for this hypothesis either because both mite prevalence and infestation intensity did not differ between solitary or aggregated individuals. Additionally, the type of aggregation (SSA or MSA) was not associated with the benefit or the cost we explored. This lack of effect may be explained by the fluid membership of the aggregations, as we found high turnover over time in the number of individuals and species composition of the aggregations. In conclusion, we hope our review and empirical data stimulate further studies on MSA, which remains one of the most elusive forms of group living in animals.},
  language  = {en}
}
@article{NathanMonkArlinghausetal.2022,
  author    = {Nathan, Ran and Monk, Christopher T. and Arlinghaus, Robert and Adam, Timo and Al{\´o}s, Josep and Assaf, Michael and Baktoft, Henrik and Beardsworth, Christine E. and Bertram, Michael G. and Bijleveld, Allert and Brodin, Tomas and Brooks, Jill L. and Campos-Candela, Andrea and Cooke, Steven J. and Gjelland, Karl O. and Gupte, Pratik R. and Harel, Roi and Hellstrom, Gustav and Jeltsch, Florian and Killen, Shaun S. and Klefoth, Thomas and Langrock, Roland and Lennox, Robert J. and Lourie, Emmanuel and Madden, Joah R. and Orchan, Yotam and Pauwels, Ine S. and Riha, Milan and R{\"o}leke, Manuel and Schl{\"a}gel, Ulrike and Shohami, David and Signer, Johannes and Toledo, Sivan and Vilk, Ohad and Westrelin, Samuel and Whiteside, Mark A. and Jaric, Ivan},
  title     = {Big-data approaches lead to an increased understanding of the ecology of animal movement},
  series = {Science},
  volume    = {375},
  journal   = {Science},
  number    = {6582},
  publisher = {American Assoc. for the Advancement of Science},
  address   = {Washington},
  issn      = {0036-8075},
  doi       = {10.1126/science.abg1780},
  pages     = {734 -- +},
  year      = {2022},
  abstract  = {Understanding animal movement is essential to elucidate how animals interact, survive, and thrive in a changing world. Recent technological advances in data collection and management have transformed our understanding of animal "movement ecology" (the integrated study of organismal movement), creating a big-data discipline that benefits from rapid, cost-effective generation of large amounts of data on movements of animals in the wild. These high-throughput wildlife tracking systems now allow more thorough investigation of variation among individuals and species across space and time, the nature of biological interactions, and behavioral responses to the environment. Movement ecology is rapidly expanding scientific frontiers through large interdisciplinary and collaborative frameworks, providing improved opportunities for conservation and insights into the movements of wild animals, and their causes and consequences.},
  language  = {en}
}
@article{ZhangHuYangetal.2022,
  author    = {Zhang, Kai and Hu, Jiege and Yang, Shuai and Xu, Wei and Wang, Zhichao and Zhuang, Peiwen and Grossart, Hans-Peter and Luo, Zhuhua},
  title     = {Biodegradation of polyester polyurethane by the marine fungus Cladosporium halotolerans 6UPA1},
  series = {Journal of hazardous materials},
  volume    = {437},
  journal   = {Journal of hazardous materials},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0304-3894},
  doi       = {10.1016/j.jhazmat.2022.129406},
  pages     = {10},
  year      = {2022},
  abstract  = {Lack of degradability and the accumulation of polymeric wastes increase the risk for the health of the environment. Recently, recycling of polymeric waste materials becomes increasingly important as raw materials for polymer synthesis are in short supply due to the rise in price and supply chain disruptions. As an important polymer, polyurethane (PU) is widely used in modern life, therefore, PU biodegradation is desirable to avoid its accumulation in the environment. In this study, we isolated a fungal strain Cladosporium halotolerans from the deep sea which can grow in mineral medium with a polyester PU (Impranil DLN) as a sole carbon source. Further, we demonstrate that it can degrade up to 80\% of Impranil PU after 3 days of incubation at 28 celcius by breaking the carbonyl groups (1732 cm(-1)) and C-N-H bonds (1532 cm(-1) and 1247 cm(-1)) as confirmed by Fourier-transform infrared (FTIR) spectroscopy analysis. Gas chromatography-mass spectrometry (GC-MS) analysis revealed polyols and alkanes as PU degradation intermediates, indicating the hydrolysis of ester and urethane bonds. Esterase and urease activities were detected in 7 days-old cultures with PU as a carbon source. Transcriptome analysis showed a number of extracellular protein genes coding for enzymes such as cutinase, lipase, peroxidase and hydrophobic surface binding proteins A (HsbA) were expressed when cultivated on Impranil PU. The yeast two-hybrid assay revealed that the hydrophobic surface binding protein ChHsbA1 directly interacts with inducible esterases, ChLip1 (lipase) and ChCut1 (cutinase). Further, the KEGG pathway for "fatty acid degradation " was significantly enriched in Impranil PU inducible genes, indicating that the fungus may use the degradation intermediates to generate energy via this pathway. Taken together, our data indicates secretion of both esterase and hydrophobic surface binding proteins by C. halotolerans plays an important role in Impranil PU absorption and subsequent degradation. Our study provides a mechanistic insight into Impranil PU biodegradation by deep sea fungi and provides the basis for future development of biotechnological PU recycling.},
  language  = {en}
}
@article{SchittkoOnandiaBernardVerdieretal.2022,
  author    = {Schittko, Conrad and Onandia, Gabriela and Bernard-Verdier, Maud and Heger, Tina and Jeschke, Jonathan M. and Kowarik, Ingo and Maaß, Stefanie and Joshi, Jasmin},
  title     = {Biodiversity maintains soil multifunctionality and soil organic carbon in novel urban ecosystems},
  series = {Journal of ecology},
  volume    = {110},
  journal   = {Journal of ecology},
  number    = {4},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0022-0477},
  doi       = {10.1111/1365-2745.13852},
  pages     = {916 -- 934},
  year      = {2022},
  abstract  = {Biodiversity in urban ecosystems has the potential to increase ecosystem functions and support a suite of services valued by society, including services provided by soils. Specifically, the sequestration of carbon in soils has often been advocated as a solution to mitigate the steady increase in CO2 concentration in the atmosphere as a key driver of climate change. However, urban ecosystems are also characterized by an often high level of ecological novelty due to profound human-mediated changes, such as the presence of high numbers of non-native species, impervious surfaces or other disturbances. Yet it is poorly understood whether and how biodiversity affects ecosystem functioning and services of urban soils under these novel conditions. In this study, we assessed the influence of above- and below-ground diversity, as well as urbanization and plant invasions, on multifunctionality and organic carbon stocks of soils in non-manipulated grasslands along an urbanization gradient in Berlin, Germany. We focused on plant diversity (measured as species richness and functional trait diversity) and, in addition, on soil organism diversity as a potential mediator for the relationship of plant species diversity and ecosystem functioning. Our results showed positive effects of plant diversity on soil multifunctionality and soil organic carbon stocks along the entire gradient. Structural equation models revealed that plant diversity enhanced soil multifunctionality and soil organic carbon by increasing the diversity of below-ground organisms. These positive effects of plant diversity on soil multifunctionality and soil fauna were not restricted to native plant species only, but were also exerted by non-native species, although to a lesser degree. Synthesis. We conclude that enhancing diversity in plants and soil fauna of urban grasslands can increase the multifunctionality of urban soils and also add to their often underestimated but very valuable role in mitigating effects of climate change.},
  language  = {en}
}
@article{IrobBlaumBaldaufetal.2022,
  author    = {Irob, Katja and Blaum, Niels and Baldauf, Selina and Kerger, Leon and Strohbach, Ben and Kanduvarisa, Angelina and Lohmann, Dirk and Tietjen, Britta},
  title     = {Browsing herbivores improve the state and functioning of savannas},
  series = {Ecology and evolution},
  volume    = {12},
  journal   = {Ecology and evolution},
  number    = {3},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {2045-7758},
  doi       = {10.1002/ece3.8715},
  pages     = {19},
  year      = {2022},
  abstract  = {Changing climatic conditions and unsustainable land use are major threats to savannas worldwide. Historically, many African savannas were used intensively for livestock grazing, which contributed to widespread patterns of bush encroachment across savanna systems. To reverse bush encroachment, it has been proposed to change the cattle-dominated land use to one dominated by comparatively specialized browsers and usually native herbivores. However, the consequences for ecosystem properties and processes remain largely unclear. We used the ecohydrological, spatially explicit model EcoHyD to assess the impacts of two contrasting, herbivore land-use strategies on a Namibian savanna: grazer- versus browser-dominated herbivore communities. We varied the densities of grazers and browsers and determined the resulting composition and diversity of the plant community, total vegetation cover, soil moisture, and water use by plants. Our results showed that plant types that are less palatable to herbivores were best adapted to grazing or browsing animals in all simulated densities. Also, plant types that had a competitive advantage under limited water availability were among the dominant ones irrespective of land-use scenario. Overall, the results were in line with our expectations: under high grazer densities, we found heavy bush encroachment and the loss of the perennial grass matrix. Importantly, regardless of the density of browsers, grass cover and plant functional diversity were significantly higher in browsing scenarios. Browsing herbivores increased grass cover, and the higher total cover in turn improved water uptake by plants overall. We concluded that, in contrast to grazing-dominated land-use strategies, land-use strategies dominated by browsing herbivores, even at high herbivore densities, sustain diverse vegetation communities with high cover of perennial grasses, resulting in lower erosion risk and bolstering ecosystem services.},
  language  = {en}
}
@article{Eccard2022,
  author    = {Eccard, Jana},
  title     = {Can rolling composite wildflower blocks increase biodiversity in agricultural landscapes better than wildflowers strips?},
  series = {Journal of applied ecology : an official journal of the British Ecological Society},
  volume    = {59},
  journal   = {Journal of applied ecology : an official journal of the British Ecological Society},
  number    = {5},
  publisher = {Wiley-Blackwell},
  address   = {Oxford},
  issn      = {0021-8901},
  doi       = {10.1111/1365-2664.14147},
  pages     = {1172 -- 1177},
  year      = {2022},
  abstract  = {Biodiversity and abundance of wildlife has dramatically declined in agricultural landscapes. Sown, short-lived wildflower (WF) strips along the margins of crop fields are a widespread and often subsidised in agri-environmental schemes, intended to enhance biodiversity, provide refuges for wild plant and arthropod populations and to provide ecosystem services to crops. Meanwhile, WF elements are also criticised, since their functionality decreases with plant succession, the removal of aged WF strip poses an ecological trap for the attracted arthropod populations and only common and mobile species benefit. Further, insects in WF strips are impacted by pesticides from agricultural fields due to shared boundaries with crop fields and by edge effects. The performance of the measure could be improved by combining several WF strips of different successional stages, each harbouring a unique community of plants and arthropods, into persistent, composite WF block, where successional stages exist in parallel. Monitoring data on many taxa in the literature shows, that a third of species are temporarily present in an ageing WF stip, thus offering composite WF blocks should increase cumulative species richness by 28\%-39\% compared to annual richness in WF strips. Persistence of composite WF blocks would offer reliable refuge for animal and plant populations, also supporting their predators and herbivores. Further, WF blocks have less boundaries to crops compared to WF strips of the same area, and are less impacted by edge effects and pesticides. Policy implications. Here I suggest a change of conservation practice changing from successional WF strips to composite WF blocks. By regular removal and replacement of aged WF strips either within the block (rotational) or at its margins (rolling), the habitat heterogeneity in composite WF block could be perpetuated. Rolling composite WF blocks change locations over years, and the original location can be reconverted to arable land while a nearby WF block is still available to wildlife. A change in agricultural schemes would be necessary, since in some European countries clustered WF strips are explicitly not subsidised.},
  language  = {en}
}
@article{MuntahaLiCompartetal.2022,
  author    = {Muntaha, Sidratul Nur and Li, Xiaoping and Compart, Julia and Apriyanto, Ardha and Fettke, J{\"o}rg},
  title     = {Carbon pathways during transitory starch degradation in Arabidopsis differentially affect the starch granule number and morphology in the dpe2/phs1 mutant background},
  series = {Plant physiology and biochemistry : an official journal of the Federation of European Societies of Plant Physiology},
  volume    = {180},
  journal   = {Plant physiology and biochemistry : an official journal of the Federation of European Societies of Plant Physiology},
  publisher = {Elsevier},
  address   = {Paris},
  issn      = {0981-9428},
  doi       = {10.1016/j.plaphy.2022.03.033},
  pages     = {35 -- 41},
  year      = {2022},
  abstract  = {The Arabidopsis knockout mutant lacking both the cytosolic disproportionating enzyme 2 (DPE2) and the plastidial phosphorylase (PHS1) had a dwarf-growth phenotype, a reduced and uneven distribution of starch within the plant rosettes, and a lower starch granule number per chloroplast under standard growth conditions. In contrast, a triple mutant impaired in starch degradation by its additional lack of the glucan, water dikinase (GWD) showed improved plant growth, a starch-excess phenotype, and a homogeneous starch distribution. Furthermore, the number of starch granules per chloroplast was increased and was similar to the wild type. We concluded that ongoing starch degradation is mainly responsible for the observed phenotype of dpe2/phs1. Next, we generated two further triple mutants lacking either the phosphoglucan, water dikinase (PWD), or the disproportionating enzyme 1 (DPE1) in the background of the double mutant. Analysis of the starch metabolism revealed that even minor ongoing starch degradation observed in dpe2/phs1/pwd maintained the double mutant phenotype. In contrast, an additional blockage in the glucose pathway of starch breakdown, as in dpe2/phs1/ dpe1, resulted in a nearly starch-free phenotype and massive chloroplast degradation. The characterized mutants were discussed in the context of starch granule formation.},
  language  = {en}
}
@article{WelkeSperberBergmannetal.2022,
  author    = {Welke, Robert-William and Sperber, Hannah Sabeth and Bergmann, Ronny and Koikkarah, Amit and Menke, Laura and Sieben, Christian and Kr{\"u}ger, Detlev H. and Chiantia, Salvatore and Herrmann, Andreas and Schwarzer, Roland},
  title     = {Characterization of hantavirus N protein intracellular dynamics and localization},
  series = {Viruses},
  volume    = {14},
  journal   = {Viruses},
  number    = {3},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {1999-4915},
  doi       = {10.3390/v14030457},
  pages     = {14},
  year      = {2022},
  abstract  = {Hantaviruses are enveloped viruses that possess a tri-segmented, negative-sense RNA genome. The viral S-segment encodes the multifunctional nucleocapsid protein (N), which is involved in genome packaging, intracellular protein transport, immunoregulation, and several other crucial processes during hantavirus infection. In this study, we generated fluorescently tagged N protein constructs derived from Puumalavirus (PUUV), the dominant hantavirus species in Central, Northern, and Eastern Europe. We comprehensively characterized this protein in the rodent cell line CHO-K1, monitoring the dynamics of N protein complex formation and investigating co-localization with host proteins as well as the viral glycoproteins Gc and Gn. We observed formation of large, fibrillar PUUV N protein aggregates, rapidly coalescing from early punctate and spike-like assemblies. Moreover, we found significant spatial correlation of N with vimentin, actin, and P-bodies but not with microtubules. N constructs also co-localized with Gn and Gc albeit not as strongly as the glycoproteins associated with each other. Finally, we assessed oligomerization of N constructs, observing efficient and concentration-dependent multimerization, with complexes comprising more than 10 individual proteins.},
  language  = {en}
}
@article{MoradianRochAnthoferetal.2022,
  author    = {Moradian, Hanieh and Roch, Toralf and Anthofer, Larissa and Lendlein, Andreas and Gossen, Manfred},
  title     = {Chemical modification of uridine modulates mRNA-mediated proinflammatory and antiviral response in primary human macrophages},
  series = {Molecular therapy},
  volume    = {27},
  journal   = {Molecular therapy},
  publisher = {Cell Press},
  address   = {Cambridge},
  issn      = {2162-2531},
  doi       = {10.1016/j.omtn.2022.01.004},
  pages     = {854 -- 869},
  year      = {2022},
  abstract  = {In vitro transcribed (IVT)-mRNA has been accepted as a promising therapeutic modality. Advances in facile and rapid production technologies make IVT-mRNA an appealing alternative to protein- or virus-based medicines. Robust expression levels, lack of genotoxicity, and their manageable immunogenicity benefit its clinical applicability. We postulated that innate immune responses of therapeutically relevant human cells can be tailored or abrogated by combinations of 5'-end and internal IVT-mRNA modifications. Using primary human macrophages as targets, our data show the particular importance of uridine modifications for IVT-mRNA performance. Among five nucleotide modification schemes tested, 5-methoxy-uridine outperformed other modifications up to 4-fold increased transgene expression, triggering moderate proinflammatory and non-detectable antiviral responses. Macrophage responses against IVT-mRNAs exhibiting high immunogenicity (e.g., pseudouridine) could be minimized upon HPLC purification. Conversely, 5'-end modifications had only modest effects on mRNA expression and immune responses. Our results revealed how the uptake of chemically modified IVT-mRNA impacts human macrophages, responding with distinct patterns of innate immune responses concomitant with increased transient transgene expression. We anticipate our findings are instrumental to predictively address specific cell responses required for a wide range of therapeutic applications from eliciting controlled immunogenicity in mRNA vaccines to, e.g., completely abrogating cell activation in protein replacement therapies.},
  language  = {en}
}
@article{WenderingNikoloski2022,
  author    = {Wendering, Philipp and Nikoloski, Zoran},
  title     = {COMMIT},
  series = {PLoS Computational Biology : a new community journal / publ. by the Public Library of Science (PLoS) in association with the International Society for Computational Biology (ISCB)},
  volume    = {18},
  journal   = {PLoS Computational Biology : a new community journal / publ. by the Public Library of Science (PLoS) in association with the International Society for Computational Biology (ISCB)},
  number    = {3},
  publisher = {Public Library of Science},
  address   = {San Fransisco},
  issn      = {1553-734X},
  doi       = {10.1371/journal.pcbi.1009906},
  pages     = {24},
  year      = {2022},
  abstract  = {Composition and functions of microbial communities affect important traits in diverse hosts, from crops to humans. Yet, mechanistic understanding of how metabolism of individual microbes is affected by the community composition and metabolite leakage is lacking. Here, we first show that the consensus of automatically generated metabolic reconstructions improves the quality of the draft reconstructions, measured by comparison to reference models. We then devise an approach for gap filling, termed COMMIT, that considers metabolites for secretion based on their permeability and the composition of the community. By applying COMMIT with two soil communities from the Arabidopsis thaliana culture collection, we could significantly reduce the gap-filling solution in comparison to filling gaps in individual reconstructions without affecting the genomic support. Inspection of the metabolic interactions in the soil communities allows us to identify microbes with community roles of helpers and beneficiaries. Therefore, COMMIT offers a versatile fully automated solution for large-scale modelling of microbial communities for diverse biotechnological applications. <br /> Author summaryMicrobial communities are important in ecology, human health, and crop productivity. However, detailed information on the interactions within natural microbial communities is hampered by the community size, lack of detailed information on the biochemistry of single organisms, and the complexity of interactions between community members. Metabolic models are comprised of biochemical reaction networks based on the genome annotation, and can provide mechanistic insights into community functions. Previous analyses of microbial community models have been performed with high-quality reference models or models generated using a single reconstruction pipeline. However, these models do not contain information on the composition of the community that determines the metabolites exchanged between the community members. In addition, the quality of metabolic models is affected by the reconstruction approach used, with direct consequences on the inferred interactions between community members. Here, we use fully automated consensus reconstructions from four approaches to arrive at functional models with improved genomic support while considering the community composition. We applied our pipeline to two soil communities from the Arabidopsis thaliana culture collection, providing only genome sequences. Finally, we show that the obtained models have 90\% genomic support and demonstrate that the derived interactions are corroborated by independent computational predictions.},
  language  = {en}
}
@article{PotenteLeveilleBourretYousefietal.2022,
  author    = {Potente, Giacomo and L{\´e}veill{\´e}-Bourret, {\´E}tienne and Yousefi, Narjes and Choudhury, Rimjhim Roy and Keller, Barbara and Diop, Seydina Issa and Duijsings, Dani{\"e}l and Pirovano, Walter and Lenhard, Michael and Sz{\"o}v{\´e}nyi, P{\´e}ter and Conti, Elena},
  title     = {Comparative genomics elucidates the origin of a supergene controlling floral heteromorphism},
  series = {Molecular biology and evolution : MBE},
  volume    = {39},
  journal   = {Molecular biology and evolution : MBE},
  number    = {2},
  publisher = {Oxford Univ. Press},
  address   = {Oxford},
  issn      = {0737-4038},
  doi       = {10.1093/molbev/msac035},
  pages     = {16},
  year      = {2022},
  abstract  = {Supergenes are nonrecombining genomic regions ensuring the coinheritance of multiple, coadapted genes. Despite the importance of supergenes in adaptation, little is known on how they originate. A classic example of supergene is the S locus controlling heterostyly, a floral heteromorphism occurring in 28 angiosperm families. In Primula, heterostyly is characterized by the cooccurrence of two complementary, self-incompatible floral morphs and is controlled by five genes clustered in the hemizygous, ca. 300-kb S locus. Here, we present the first chromosome-scale genome assembly of any heterostylous species, that of Primula veris (cowslip). By leveraging the high contiguity of the P. veris assembly and comparative genomic analyses, we demonstrated that the S-locus evolved via multiple, asynchronous gene duplications and independent gene translocations. Furthermore, we discovered a new whole-genome duplication in Ericales that is specific to the Primula lineage. We also propose a mechanism for the origin of S-locus hemizygosity via nonhomologous recombination involving the newly discovered two pairs of CFB genes flanking the S locus. Finally, we detected only weak signatures of degeneration in the S locus, as predicted for hemizygous supergenes. The present study provides a useful resource for future research addressing key questions on the evolution of supergenes in general and the S locus in particular: How do supergenes arise? What is the role of genome architecture in the evolution of complex adaptations? Is the molecular architecture of heterostyly supergenes across angiosperms similar to that of Primula?},
  language  = {en}
}
@article{WeithoffBell2022,
  author    = {Weithoff, Guntram and Bell, Elanor Margaret},
  title     = {Complex Trophic Interactions in an Acidophilic Microbial Community},
  series = {Microorganisms},
  volume    = {10},
  journal   = {Microorganisms},
  edition   = {7},
  publisher = {MDPI},
  address   = {Basel, Schweiz},
  issn      = {2076-2607},
  doi       = {10.3390/microorganisms10071340},
  pages     = {1 -- 10},
  year      = {2022},
  abstract  = {Extreme habitats often harbor specific communities that differ substantially from non-extreme habitats. In many cases, these communities are characterized by archaea, bacteria and protists, whereas the number of species of metazoa and higher plants is relatively low. In extremely acidic habitats, mostly prokaryotes and protists thrive, and only very few metazoa thrive, for example, rotifers. Since many studies have investigated the physiology and ecology of individual species, there is still a gap in research on direct, trophic interactions among extremophiles. To fill this gap, we experimentally studied the trophic interactions between a predatory protist (Actinophrys sol, Heliozoa) and its prey, the rotifers Elosa woralli and Cephalodella sp., the ciliate Urosomoida sp. and the mixotrophic protist Chlamydomonas acidophila (a green phytoflagellate, Chlorophyta). We found substantial predation pressure on all animal prey. High densities of Chlamydomonas acidophila reduced the predation impact on the rotifers by interfering with the feeding behaviour of A. sol. These trophic relations represent a natural case of intraguild predation, with Chlamydomonas acidophila being the common prey and the rotifers/ciliate and A. sol being the intraguild prey and predator, respectively. We further studied this intraguild predation along a resource gradient using Cephalodella sp. as the intraguild prey. The interactions among the three species led to an increase in relative rotifer abundance with increasing resource (Chlamydomonas) densities. By applying a series of laboratory experiments, we revealed the complexity of trophic interactions within a natural extremophilic community.},
  language  = {en}
}
@article{GutschmannSimoesSchieweetal.2022,
  author    = {Gutschmann, Bj{\"o}rn and Sim{\~o}es, Matilde Maldonado and Schiewe, Thomas and Schr{\"o}ter, Edith S. and M{\"u}nzberg, Marvin and Neubauer, Peter and Bockisch, Anika and Riedel, Sebastian Lothar Stefan},
  title     = {Continuous feeding strategy for polyhydroxyalkanoate production from solid waste animal fat at laboratory- and pilot-scale},
  series = {Microbial biotechnology / Society for Applied Microbiology},
  journal   = {Microbial biotechnology / Society for Applied Microbiology},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {1751-7915},
  doi       = {10.1111/1751-7915.14104},
  pages     = {12},
  year      = {2022},
  abstract  = {Bioconversion of waste animal fat (WAF) to polyhydroxyalkanoates (PHAs) is an approach to lower the production costs of these plastic alternatives. However, the solid nature of WAF requires a tailor-made process development. In this study, a double-jacket feeding system was built to thermally liquefy the WAF to employ a continuous feeding strategy. During laboratory-scale cultivations with Ralstonia eutropha Re2058/pCB113, 70\% more PHA (45 g(PHA) L-1) and a 75\% higher space-time yield (0.63 g(PHA) L-1 h(-1)) were achieved compared to previously reported fermentations with solid WAF. During the development process, growth and PHA formation were monitored in real-time by in-line photon density wave spectroscopy. The process robustness was further evaluated during scale-down fermentations employing an oscillating aeration, which did not alter the PHA yield although cells encountered periods of oxygen limitation. Flow cytometry with propidium iodide staining showed that more than two-thirds of the cells were viable at the end of the cultivation and viability was even little higher in the scale-down cultivations. Application of this feeding system at 150-L pilot-scale cultivation yielded in 31.5 g(PHA) L-1, which is a promising result for the further scale-up to industrial scale.},
  language  = {en}
}
@article{Heinze2022,
  author    = {Heinze, Johannes},
  title     = {Correction to: Heinze, Johannes: Herbivory by aboveground insects impacts plant root morphological traits. - Plant Ecology. - 221 (2020). - S. 725 - 732},
  series = {Plant ecology : an international journal},
  volume    = {223},
  journal   = {Plant ecology : an international journal},
  number    = {115},
  publisher = {Springer},
  address   = {Dordrecht},
  issn      = {1385-0237},
  doi       = {10.1007/s11258-021-01194-6},
  year      = {2022},
  language  = {en}
}
@article{OmranianNikoloski2022,
  author    = {Omranian, Sara and Nikoloski, Zoran},
  title     = {CUBCO+: prediction of protein complexes based on min-cut network partitioning into biclique spanned subgraphs},
  series = {Applied Network Science},
  volume    = {7},
  journal   = {Applied Network Science},
  publisher = {Springer International Publishing},
  address   = {Cham},
  issn      = {2364-8228},
  doi       = {10.1007/s41109-022-00508-5},
  pages     = {12},
  year      = {2022},
  abstract  = {High-throughput proteomics approaches have resulted in large-scale protein-protein interaction (PPI) networks that have been employed for the prediction of protein complexes. However, PPI networks contain false-positive as well as false-negative PPIs that affect the protein complex prediction algorithms. To address this issue, here we propose an algorithm called CUBCO+ that: (1) employs GO semantic similarity to retain only biologically relevant interactions with a high similarity score, (2) based on link prediction approaches, scores the false-negative edges, and (3) incorporates the resulting scores to predict protein complexes. Through comprehensive analyses with PPIs from Escherichia coli, Saccharomyces cerevisiae, and Homo sapiens, we show that CUBCO+ performs as well as the approaches that predict protein complexes based on recently introduced graph partitions into biclique spanned subgraphs and outperforms the other state-of-the-art approaches. Moreover, we illustrate that in combination with GO semantic similarity, CUBCO+ enables us to predict more accurate protein complexes in 36\% of the cases in comparison to CUBCO as its predecessor.},
  language  = {en}
}
@article{KindermannDoblerNiedeggenetal.2022,
  author    = {Kindermann, Liana and Dobler, Magnus and Niedeggen, Daniela and Chimbioputo Fabiano, Ezequiel and Linst{\"a}dter, Anja},
  title     = {Dataset on woody aboveground biomass, disturbance losses, and wood density from an African savanna ecosystem},
  series = {Data in Brief},
  volume    = {42},
  journal   = {Data in Brief},
  publisher = {Elsevier},
  address   = {Amsterdam, Niederlande},
  issn      = {2352-3409},
  doi       = {10.1016/j.dib.2022.108155},
  pages     = {1 -- 16},
  year      = {2022},
  abstract  = {This dataset comprises tree inventories and damage assessments performed in Namibia's semi-arid Zambezi Region. Data were sampled in savannas and savanna woodlands along steep gradients of elephant population densities to capture the effects of those (and other) disturbances on individual-level and stand-level aboveground woody biomass (AGB). The dataset contains raw data on dendrometric measures and processed data on specific wood density (SWD), woody aboveground biomass, and biomass losses through disturbance impacts. Allometric proxies (height, canopy diameters, and in adult trees also stem circumferences) were recorded for n = 6,179 tree and shrub individuals. Wood samples were taken for each encountered species to measure specific wood density. These measurements have been used to estimate woody aboveground biomass via established allometric models, advanced through our improved methodologies and workflows that accounted for tree and shrub architecture shaped by disturbance impacts. To this end, we performed a detailed damage assessment on each woody individual in the field. In addition to estimations of standing biomass, our new method also delivered data on biomass losses to different disturbance agents (elephants, fire, and others) on the level of plant individuals and stands. The data presented here have been used within a study published with Ecological Indicators (Kindermann et al., 2022) to evaluate the benefits of our improved methodology in comparison to a standard reference method of aboveground biomass estimations. Additionally, it has been employed in a study on carbon storage and sequestration in vegetation and soils (Sandhage-Hofmann et al., 2021). The raw data of dendrometric measurements can be subjected to other available allometric models for biomass estimation. The processed data can be used to analyze disturbance impacts on woody aboveground biomass, or for regional carbon storage estimates. The data on species-specific wood density can be used for application to other dendrometric datasets to (re-) estimate biomass through allometric models requiring wood density. It can further be used for plant functional trait analyses.},
  language  = {en}
}
@article{KrumbholzIshidaBaunachetal.2022,
  author    = {Krumbholz, Julia and Ishida, Keishi and Baunach, Martin and Teikari, Jonna and Rose, Magdalena M. and Sasso, Severin and Hertweck, Christian and Dittmann, Elke},
  title     = {Deciphering chemical mediators regulating specialized metabolism in a symbiotic cyanobacterium},
  series = {Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker. International edition},
  journal   = {Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker. International edition},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1433-7851},
  doi       = {10.1002/anie.202204545},
  pages     = {10},
  year      = {2022},
  abstract  = {Genomes of cyanobacteria feature a variety of cryptic biosynthetic pathways for complex natural products, but the peculiarities limiting the discovery and exploitation of the metabolic dark matter are not well understood. Here we describe the discovery of two cell density-dependent chemical mediators, nostoclide and nostovalerolactone, in the symbiotic model strain Nostoc punctiforme, and demonstrate their pronounced impact on the regulation of specialized metabolism. Through transcriptional, bioinformatic and labeling studies we assigned two adjacent biosynthetic gene clusters to the biosynthesis of the two polyketide mediators. Our findings provide insight into the orchestration of specialized metabolite production and give lessons for the genomic mining and high-titer production of cyanobacterial bioactive compounds.},
  language  = {en}
}
@article{SoutoVeigaGroeneveldEnrightetal.2022,
  author    = {Souto-Veiga, Rodrigo and Groeneveld, Juergen and Enright, Neal J. and Fontaine, Joseph B. and Jeltsch, Florian},
  title     = {Declining pollination success reinforces negative climate and fire change impacts in a serotinous, fire-killed plant},
  series = {Plant ecology : an international journal},
  volume    = {223},
  journal   = {Plant ecology : an international journal},
  number    = {7},
  publisher = {Springer},
  address   = {Dordrecht},
  issn      = {1385-0237},
  doi       = {10.1007/s11258-022-01244-7},
  pages     = {863 -- 881},
  year      = {2022},
  abstract  = {Climate change projections predict that Mediterranean-type ecosystems (MTEs) are becoming hotter and drier and that fires will become more frequent and severe. While most plant species in these important biodiversity hotspots are adapted to hot, dry summers and recurrent fire, the Interval Squeeze framework suggests that reduced seed production (demographic shift), reduced seedling establishment after fire (post fire recruitment shift), and reduction in the time between successive fires (fire interval shift) will threaten fire killed species under climate change. One additional potential driver of accelerated species decline, however, has not been considered so far: the decrease in pollination success observed in many ecosystems worldwide has the potential to further reduce seed accumulation and thus population persistence also in these already threatened systems. Using the well-studied fire-killed and serotinous shrub species Banksia hookeriana as an example, we apply a new spatially implicit population simulation model to explore population dynamics under past (1988-2002) and current (2003-2017) climate conditions, deterministic and stochastic fire regimes, and alternative scenarios of pollination decline. Overall, model results suggest that while B. hookeriana populations were stable under past climate conditions, they will not continue to persist under current (and prospective future) climate. Negative effects of climatic changes and more frequent fires are reinforced by the measured decline in seed set leading to further reduction in the mean persistence time by 12-17\%. These findings clearly indicate that declining pollination rates can be a critical factor that increases further the pressure on the persistence of fire-killed plants. Future research needs to investigate whether other fire-killed species are similarly threatened, and if local population extinction may be compensated by recolonization events, facilitating persistence in spatially structured meta-communities.},
  language  = {en}
}
@article{TolomeevDubovskayaKirillinetal.2022,
  author    = {Tolomeev, Aleksandr P. and Dubovskaya, Olga P. and Kirillin, Georgiy and Buseva, Zhanna and Kolmakova, Olesya and Grossart, Hans-Peter and Tang, Kam W. and Gladyšev, Michail I.},
  title     = {Degradation of dead cladoceran zooplankton and their contribution to organic carbon cycling in stratified lakes},
  series = {Journal of plankton research},
  volume    = {44},
  journal   = {Journal of plankton research},
  number    = {3},
  publisher = {Oxford Univ. Press},
  address   = {Oxford},
  issn      = {0142-7873},
  doi       = {10.1093/plankt/fbac023},
  pages     = {386 -- 400},
  year      = {2022},
  abstract  = {The contribution of dead zooplankton biomass to carbon cycle in aquatic ecosystems is practically unknown. Using abundance data of zooplankton in water column and dead zooplankton in sediment traps in Lake Stechlin, an ecological-mathematical model was developed to simulate the abundance and sinking of zooplankton carcasses and predict the related release of labile organic matter (LOM) into the water column. We found species-specific differences in mortality rate of the dominant zooplankton: Daphnia cucullata, Bosmina coregoni and Diaphanosoma brachyurum (0.008, 0.129 and 0.020 day(-1), respectively) and differences in their carcass sinking velocities in metalimnion (and hypolimnion): 2.1 (7.64), 14.0 (19.5) and 1.1 (5.9) m day(-1), respectively. Our model simulating formation and degradation processes of dead zooplankton predicted a bimodal distribution of the released LOM: epilimnic and metalimnic peaks of comparable intensity, ca. 1 mg DW m(-3) day(-1). Maximum degradation of carcasses up to ca. 1.7 mg DW m(-3) day(-1) occurred in the density gradient zone of metalimnion. LOM released from zooplankton carcasses into the surrounding water may stimulate microbial activity and facilitate microbial degradation of more refractory organic matter; therefore, dead zooplankton are expected to be an integral part of water column carbon source/sink dynamics in stratified lakes.},
  language  = {en}
}
@article{BoekerHermanussenScheffler2022,
  author    = {Boeker, Sonja and Hermanussen, Michael and Scheffler, Christiane},
  title     = {Dental age is an independent marker of biological age},
  series = {Human biology and public health},
  volume    = {2021},
  journal   = {Human biology and public health},
  number    = {3, Summer School Supplement},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  issn      = {2748-9957},
  doi       = {10.52905/hbph2021.3.24},
  pages     = {9},
  year      = {2022},
  abstract  = {Background: Biological age markers are a crucial indicator whether children are decelerated in growth tempo. Skeletal maturation is the standard measure. Yet, it relies on exposing children to x-radiation. Dental eruption is a potential, but highly debated, radiation free alternative.  Objectives: We assess the interrelationship between dental eruption and other maturational markers. We hypothesize that dental age correlates with body height and skeletal age. We further evaluate how the three different variables behave in cohorts from differing social backgrounds. Sample and Method: Dental, skeletal and height data from the 1970s to 1990s from Guatemalan boys were converted into standard deviation scores, using external references for each measurement. The boys, aged between 7 and 12, derived from different social backgrounds (middle SES (N = 6529), low-middle SES (N = 736), low SES Ladino (N = 3653) and low SES Maya (N = 4587). Results: Dental age shows only a weak correlation with skeletal age (0.18) and height (0.2). The distinction between cohorts differs according to each of the three measurements. All cohorts differ significantly in height. In skeletal maturation, the middle SES cohort is significantly advanced compared to all other cohorts. The periodically malnourished cohorts of low SES Mayas and Ladinos are significantly delayed in dental maturation compared to the well-nourished low-middle and middle class Ladino children. Conclusion: Dental development is an independent system, that is regulated by different mechanisms than skeletal development and growth. Tooth eruption is sensitive to nutritional status, whereas skeletal age is more sensitive to socioeconomic background.},
  language  = {en}
}
@article{SauterKratzFarhanetal.2022,
  author    = {Sauter, Tilman and Kratz, Karl and Farhan, Muhammad and Heuchel, Matthias and Lendlein, Andreas},
  title     = {Design and fabrication of fiber mesh actuators},
  series = {Applied materials today},
  volume    = {29},
  journal   = {Applied materials today},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {2352-9407},
  doi       = {10.1016/j.apmt.2022.101562},
  pages     = {13},
  year      = {2022},
  abstract  = {Soft actuator performance can be tuned by chemistry or mechanical manipulation, but this adjustability is limited especially in view of their growing technological relevance. Inspired from textile engineering, we designed and fabricated fiber mesh actuators and introduced new features like anisotropic behavior and soft-tissue like elastic deformability. Design criteria for the meshes are the formation of fiber bundles, the angle between fiber bundles in different stacked layers and covalent crosslinks forming within and between fibers at their interfacial contact areas. Through crosslinking the interfiber bond strength increased from a bond transmitting neither axial nor rotational loads (pin joint) to a bond strength capable of both (welded joint). For non-linear elastic stiffening, stacked fiber bundles with four embracing fibers were created forming microstructural rhombus shapes. Loading the rhombus diagonally allowed generation of "soft tissue"-like mechanics. By adjustment of stacking angles, the point of strong increase in stress is tuned. While the highest stresses are observed in aligned and crosslinked fiber mats along the direction of the fiber, the strongest shape-memory actuation behavior is found in randomly oriented fiber mats. Fiber mesh actuators controlled by temperature are of high significance as soft robot skins and as for active patches supporting tissue regeneration.},
  language  = {en}
}
@article{AzcorraDickinsonMendezDominguezetal.2022,
  author    = {Azcorra, Hugo and Dickinson, Federico and Mendez-Dominguez, Nina and Mumm, Rebekka and Valent{\´i}n, Graciela},
  title     = {Development of birthweight and length for gestational age and sex references in Yucatan, Mexico},
  series = {American journal of human biology : the official journal of the Human Biology Council},
  volume    = {34},
  journal   = {American journal of human biology : the official journal of the Human Biology Council},
  number    = {6},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {1042-0533},
  doi       = {10.1002/ajhb.23732},
  pages     = {13},
  year      = {2022},
  abstract  = {Objective To develop sex- and gestational age specific reference percentiles and curves for birth weight and length for Yucatec neonates using data from birth registers of infants born during 2015-2019. Material and methods Observational, descriptive, epidemiologic study in a 5-year period including every registered birth in the state of Yucatan, Mexico using birth registries. A total of 158 432 live, physically healthy singletons (76 442 females and 81 990 males) between 25 and 42 weeks of gestation were included in the analysis. We used the LMS method to construct smoothed reference centiles (3rd, 10th, 25th, 50th, 75th, 95th, and 97th) and curves for males and females separately. Results Mean maternal age was 26 (SD = 6.22) years. Fifty-two percent of births occurred by vaginal delivery, 37\% were firstborn and similar proportions were second (33\%) and third or more (30\%) born. 5.5\% of newborns included in the references corresponds to neonates born before 37 weeks of gestation (5.9\% boys and 5.1\% girls). In both sexes, the percentage of infants with a birthweight less than 2500 g was 6.7\%. The birthweight at the 50th percentile for males and females at 40 weeks of gestation in this cohort was 3256 and 3167 g, respectively, and the corresponding values for birth length were 50.23 and 49.84 cm (mean differences between sexes: 89 g and 0.40 cm, respectively). Conclusion The reference percentile and curves developed in this study are useful for research purposes and can help health practitioners to assess the biological status of infants born in Yucatan.},
  language  = {en}
}
@article{SchweigelBatsiosMuellerTaubenbergeretal.2022,
  author    = {Schweigel, Ulrike and Batsios, Petros and M{\"u}ller-Taubenberger, Annette and Gr{\"a}f, Ralph and Grafe, Marianne},
  title     = {Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis},
  series = {Nucleus},
  volume    = {13},
  journal   = {Nucleus},
  number    = {1},
  publisher = {Taylor \& Francis Group},
  address   = {Philadelphia},
  issn      = {1949-1034},
  doi       = {10.1080/19491034.2022.2047289},
  pages     = {144 -- 154},
  year      = {2022},
  abstract  = {Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae.},
  language  = {en}
}
@article{BiermannBachKlaeringetal.2022,
  author    = {Biermann, Robin Tim and Bach, Linh T. and Kl{\"a}ring, Hans-Peter and Baldermann, Susanne and B{\"o}rnke, Frederik and Schwarz, Dietmar},
  title     = {Discovering tolerance-A computational approach to assess abiotic stress tolerance in tomato under greenhouse conditions},
  series = {Frontiers in sustainable food systems},
  volume    = {6},
  journal   = {Frontiers in sustainable food systems},
  publisher = {Frontiers Media},
  address   = {Lausanne},
  issn      = {2571-581X},
  doi       = {10.3389/fsufs.2022.878013},
  pages     = {12},
  year      = {2022},
  abstract  = {Modern plant cultivars often possess superior growth characteristics, but within a limited range of environmental conditions. Due to climate change, crops will be exposed to distressing abiotic conditions more often in the future, out of which heat stress is used as example for this study. To support identification of tolerant germplasm and advance screening techniques by a novel multivariate evaluation method, a diversity panel of 14 tomato genotypes, comprising Mediterranean landraces of Solanum lycopersicum, the cultivar "Moneymaker" and Solanum pennellii LA0716, which served as internal references, was assessed toward their tolerance against long-term heat stress. After 5 weeks of growth, young tomato plants were exposed to either control (22/18 degrees C) or heat stress (35/25 degrees C) conditions for 2 weeks. Within this period, water consumption, leaf angles and leaf color were determined. Additionally, gas exchange and leaf temperature were investigated. Finally, biomass traits were recorded. The resulting multivariate dataset on phenotypic plasticity was evaluated to test the hypothesis, that more tolerant genotypes have less affected phenotypes upon stress adaptation. For this, a cluster-analysis-based approach was developed that involved a principal component analysis (PCA), dimension reduction and determination of Euclidean distances. These distances served as measure for the phenotypic plasticity upon heat stress. Statistical evaluation allowed the identification and classification of homogeneous groups consisting each of four putative more or less heat stress tolerant genotypes. The resulting classification of the internal references as "tolerant" highlights the applicability of our proposed tolerance assessment model. PCA factor analysis on principal components 1-3 which covered 76.7\% of variance within the phenotypic data, suggested that some laborious measure such as the gas exchange might be replaced with the determination of leaf temperature in larger heat stress screenings. Hence, the overall advantage of the presented method is rooted in its suitability of both, planning and executing screenings for abiotic stress tolerance using multivariate phenotypic data to overcome the challenge of identifying abiotic stress tolerant plants from existing germplasms and promote sustainable agriculture for the future.},
  language  = {en}
}
@article{KiemelWeithoffTiedemann2022,
  author    = {Kiemel, Katrin and Weithoff, Guntram and Tiedemann, Ralph},
  title     = {DNA metabarcoding reveals impact of local recruitment, dispersal, and hydroperiod on assembly of a zooplankton metacommunity},
  series = {Molecular ecology},
  volume    = {32},
  journal   = {Molecular ecology},
  number    = {23},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0962-1083},
  doi       = {10.1111/mec.16627},
  pages     = {20},
  year      = {2022},
  abstract  = {Understanding the environmental impact on the assembly of local communities in relation to their spatial and temporal connectivity is still a challenge in metacommunity ecology. This study aims to unravel underlying metacommunity processes and environmental factors that result in observed zooplankton communities. Unlike most metacommunity studies, we jointly examine active and dormant zooplankton communities using a DNA metabarcoding approach to overcome limitations of morphological species identification. We applied two-fragment (COI and 18S) metabarcoding to monitor communities of 24 kettle holes over a two-year period to unravel (i) spatial and temporal connectivity of the communities, (ii) environmental factors influencing local communities, and (iii) dominant underlying metacommunity processes in this system. We found a strong separation of zooplankton communities from kettle holes of different hydroperiods (degree of permanency) throughout the season, while the community composition within single kettle holes did not differ between years. Species richness was primarily dependent on pH and permanency, while species diversity (Shannon Index) was influenced by kettle hole location. Community composition was impacted by kettle hole size and surrounding field crops. Environmental processes dominated temporal and spatial processes. Sediment communities showed a different composition compared to water samples but did not differ between ephemeral and permanent kettle holes. Our results suggest that communities are mainly structured by environmental filtering based on pH, kettle hole size, surrounding field crops, and permanency. Environmental filtering based on specific conditions in individual kettle holes seems to be the dominant process in community assembly in the studied zooplankton metacommunity.},
  language  = {en}
}
@article{ScharfWeineltSchroederetal.2022,
  author    = {Scharf, Christina and Weinelt, Ferdinand Anton and Schroeder, Ines and Paal, Michael and Weigand, Michael and Zoller, Michael and Irlbeck, Michael and Kloft, Charlotte and Briegel, Josef and Liebchen, Uwe},
  title     = {Does the cytokine adsorber CytoSorb (R) reduce vancomycin exposure in critically ill patients with sepsis or septic shock?},
  series = {Annals of intensive care},
  volume    = {12},
  journal   = {Annals of intensive care},
  number    = {1},
  publisher = {Springer},
  address   = {Heidelberg},
  issn      = {2110-5820},
  doi       = {10.1186/s13613-022-01017-5},
  pages     = {8},
  year      = {2022},
  abstract  = {Background: Hemadsorption of cytokines is used in critically ill patients with sepsis or septic shock. Concerns have been raised that the cytokine adsorber CytoSorb (R) unintentionally adsorbs vancomycin. This study aimed to quantify vancomycin elimination by CytoSorb (R) . Methods: Critically ill patients with sepsis or septic shock receiving continuous renal replacement therapy and CytoSorb (R) treatment during a prospective observational study were included in the analysis. Vancomycin pharmacokinetics was characterized using population pharmacokinetic modeling. Adsorption of vancomycin by the CytoSorb (R) was investigated as linear or saturable process. The final model was used to derive dosing recommendations based on stochastic simulations. Results: 20 CytoSorb (R) treatments in 7 patients (160 serum samples/24 during CytoSorb (R)-treatment, all continuous infusion) were included in the study. A classical one-compartment model, including effluent flow rate of the continuous hemodialysis as linear covariate on clearance, best described the measured concentrations (without CytoSorb (R)). Significant adsorption with a linear decrease during CytoSorb (R) treatment was identified (p <0.0001) and revealed a maximum increase in vancomycin clearance of 291\% (initially after CytoSorb (R) installation) and a maximum adsorption capacity of 572 mg. For a representative patient of our cohort a reduction of the area under the curve (AUC) by 93 mg/L*24 h during CytoSorb (R) treatment was observed. The additional administration of 500 mg vancomycin over 2 h during CytoSorb (R) attenuated the effect and revealed a negligible reduction of the AUC by 4 mg/L*24h. Conclusion: We recommend the infusion of 500 mg vancomycin over 2 h during CytoSorb (R) treatment to avoid subtherapeutic concentrations.},
  language  = {en}
}
@article{HeringHauptfleischJagoetal.2022,
  author    = {Hering, Robert and Hauptfleisch, Morgan and Jago, Mark and Smith, Taylor and Kramer-Schadt, Stephanie and Stiegler, Jonas and Blaum, Niels},
  title     = {Don't stop me now: Managed fence gaps could allow migratory ungulates to track dynamic resources and reduce fence related energy loss},
  series = {Frontiers in Ecology and Evolution},
  journal   = {Frontiers in Ecology and Evolution},
  publisher = {Frontiers},
  address   = {Lausanne, Schweiz},
  issn      = {2296-701X},
  doi       = {10.3389/fevo.2022.907079},
  pages     = {1 -- 18},
  year      = {2022},
  abstract  = {In semi-arid environments characterized by erratic rainfall and scattered primary production, migratory movements are a key survival strategy of large herbivores to track resources over vast areas. Veterinary Cordon Fences (VCFs), intended to reduce wildlife-livestock disease transmission, fragment large parts of southern Africa and have limited the movements of large wild mammals for over 60 years. Consequently, wildlife-fence interactions are frequent and often result in perforations of the fence, mainly caused by elephants. Yet, we lack knowledge about at which times fences act as barriers, how fences directly alter the energy expenditure of native herbivores, and what the consequences of impermeability are. We studied 2-year ungulate movements in three common antelopes (springbok, kudu, eland) across a perforated part of Namibia's VCF separating a wildlife reserve and Etosha National Park using GPS telemetry, accelerometer measurements, and satellite imagery. We identified 2905 fence interaction events which we used to evaluate critical times of encounters and direct fence effects on energy expenditure. Using vegetation type-specific greenness dynamics, we quantified what animals gained in terms of high quality food resources from crossing the VCF. Our results show that the perforation of the VCF sustains herbivore-vegetation interactions in the savanna with its scattered resources. Fence permeability led to peaks in crossing numbers during the first flush of woody plants before the rain started. Kudu and eland often showed increased energy expenditure when crossing the fence. Energy expenditure was lowered during the frequent interactions of ungulates standing at the fence. We found no alteration of energy expenditure when springbok immediately found and crossed fence breaches. Our results indicate that constantly open gaps did not affect energy expenditure, while gaps with obstacles increased motion. Closing gaps may have confused ungulates and modified their intended movements. While browsing, sedentary kudu's use of space was less affected by the VCF; migratory, mixed-feeding springbok, and eland benefited from gaps by gaining forage quality and quantity after crossing. This highlights the importance of access to vast areas to allow ungulates to track vital vegetation patches.},
  language  = {en}
}
@article{LiApriyantoFloresCastellanosetal.2022,
  author    = {Li, Xiaoping and Apriyanto, Ardha and Flores Castellanos, Junio and Compart, Julia and Muntaha, Sidratul Nur and Fettke, J{\"o}rg},
  title     = {Dpe2/phs1 revealed unique starch metabolism with three distinct phases characterized by different starch granule numbers per chloroplast, allowing insights into the control mechanism of granule number regulation by gene co-regulation and metabolic profiling},
  series = {Frontiers in Plant Science},
  journal   = {Frontiers in Plant Science},
  publisher = {Frontiers},
  address   = {Lausanne, Schweiz},
  issn      = {1664-462X},
  doi       = {10.3389/fpls.2022.1039534},
  pages     = {1 -- 16},
  year      = {2022},
  abstract  = {An Arabidopsis mutant lacking both the cytosolic Disproportionating enzyme 2 (DPE2) and the plastidial glucan Phosphorylase 1 (PHS1) revealed a unique starch metabolism. Dpe2/phs1 has been reported to have only one starch granule number per chloroplast when grown under diurnal rhythm. For this study, we analyzed dpe2/phs1 in details following the mutant development, and found that it showed three distinct periods of granule numbers per chloroplast, while there was no obvious change observed in Col-0. In young plants, the starch granule number was similar to that in Col-0 at first, and then decreased significantly, down to one or no granule per chloroplast, followed by an increase in the granule number. Thus, in dpe2/phs1, control over the starch granule number is impaired, but it is not defective in starch granule initiation. The data also indicate that the granule number is not fixed, and is regulated throughout plant growth. Furthermore, the chloroplasts revealed alterations during these three periods, with a partially strong aberrant morphology in the middle phase. Interestingly, the unique metabolism was perpetuated when starch degradation was further impaired through an additional lack of Isoamylase 3 (ISA3) or Starch excess 4 (SEX4). Transcriptomic studies and metabolic profiling revealed the co-regulation of starch metabolism-related genes and a clear metabolic separation between the periods. Most senescence-induced genes were found to be up-regulated more than twice in the starch-less mature leaves. Thus, dpe2/phs1 is a unique plant material source, with which we may study starch granule number regulation to obtain a more detailed understanding.},
  language  = {en}
}
@article{HeringHauptfleischKramerSchadtetal.2022,
  author    = {Hering, Robert and Hauptfleisch, Morgan and Kramer-Schadt, Stephanie and Stiegler, Jonas and Blaum, Niels},
  title     = {Effects of fences and fence gaps on the movement behavior of three southern African antelope species},
  series = {Frontiers in Conservation Science},
  volume    = {3},
  journal   = {Frontiers in Conservation Science},
  publisher = {Frontiers},
  address   = {Lausanne, Schweiz},
  issn      = {2673-611X},
  doi       = {10.3389/fcosc.2022.959423},
  pages     = {1 -- 19},
  year      = {2022},
  abstract  = {Globally, migratory ungulates are affected by fences. While field observational studies reveal the amount of animal-fence interactions across taxa, GPS tracking-based studies uncover fence effects on movement patterns and habitat selection. However, studies on the direct effects of fences and fence gaps on movement behavior, especially based on high-frequency tracking data, are scarce. We used GPS tracking on three common African antelopes (Tragelaphus strepsiceros, Antidorcas marsupialis, and T. oryx) with movement strategies ranging from range residency to nomadism in a semi-arid, Namibian savanna traversed by wildlife-proof fences that elephants have regularly breached. We classified major forms of ungulate-fence interaction types on a seasonal and a daily scale. Furthermore, we recorded the distances and times spent at fences regarding the total individual space use. Based on this, we analyzed the direct effects of fences and fence gaps on the animals' movement behavior for the previously defined types of animal-fence interactions. Antelope-fence interactions peaked during the early hours of the day and during seasonal transitions when the limiting resource changed between water and forage. Major types of ungulate-fence interactions were quick, trace-like, or marked by halts. We found that the amount of time spent at fences was highest for nomadic eland. Migratory springbok adjusted their space use concerning fence gap positions. If the small home ranges of sedentary kudu included a fence, they frequently interacted with this fence. For springbok and eland, distance traveled along a fence declined with increasing utilization of a fence gap. All species reduced their speed in the proximity of a fence but often increased their speed when encountering the fence. Crossing a fence led to increased speeds for all species. We demonstrate that fence effects mainly occur during crucial foraging times (seasonal scale) and during times of directed movements (daily scale). Importantly, we provide evidence that fences directly alter antelope movement behaviors with negative implications for energy budgets and that persistent fence gaps can reduce the intensity of such alterations. Our findings help to guide future animal-fence studies and provide insights for wildlife fencing and fence gap planning.},
  language  = {en}
}
@article{HanniganNendelKrull2022,
  author    = {Hannigan, Sara and Nendel, Claas and Krull, Marcos},
  title     = {Effects of temperature on the movement and feeding behaviour of the large lupine beetle, Sitona gressorius},
  series = {Journal of pest science},
  journal   = {Journal of pest science},
  publisher = {Springer},
  address   = {Heidelberg},
  issn      = {1612-4758},
  doi       = {10.1007/s10340-022-01510-7},
  pages     = {389 -- 402},
  year      = {2022},
  abstract  = {Even though the effects of insect pests on global agricultural productivity are well recognised, little is known about movement and dispersal of many species, especially in the context of global warming. This work evaluates how temperature and light conditions affect different movement metrics and the feeding rate of the large lupine beetle, an agricultural pest responsible for widespread damage in leguminous crops. By using video recordings, the movement of 384 beetles was digitally analysed under six different temperatures and light conditions in the laboratory. Bayesian linear mixed-effect models were used to analyse the data. Furthermore, the effects of temperature on the daily diffusion coefficient of beetles were estimated by using hidden Markov models and random walk simulations. Results of this work show that temperature, light conditions, and beetles' weight were the main factors affecting the flight probability, displacement, time being active and the speed of beetles. Significant variations were also observed in all evaluated metrics. On average, beetles exposed to light conditions and higher temperatures had higher mean speed and flight probability. However, beetles tended to stay more active at higher temperatures and less active at intermediate temperatures, around 20 degrees C. Therefore, both the diffusion coefficient and displacement of beetles were lower at intermediate temperatures. These results show that the movement behaviour and feeding rates of beetles can present different relationships in the function of temperature. It also shows that using a single diffusion coefficient for insects in spatially explicit models may lead to over- or underestimation of pest spread.},
  language  = {en}
}
@article{PerrellaBaeurlevanZanten2022,
  author    = {Perrella, Giorgio and B{\"a}urle, Isabel and van Zanten, Martijn},
  title     = {Epigenetic regulation of thermomorphogenesis and heat stress tolerance},
  series = {New phytologist : international journal of plant science},
  volume    = {234},
  journal   = {New phytologist : international journal of plant science},
  number    = {4},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0028-646X},
  doi       = {10.1111/nph.17970},
  pages     = {1144 -- 1160},
  year      = {2022},
  abstract  = {Many environmental conditions fluctuate and organisms need to respond effectively. This is especially true for temperature cues that can change in minutes to seasons and often follow a diurnal rhythm. Plants cannot migrate and most cannot regulate their temperature. Therefore, a broad array of responses have evolved to deal with temperature cues from freezing to heat stress. A particular response to mildly elevated temperatures is called thermomorphogenesis, a suite of morphological adaptations that includes thermonasty, formation of thin leaves and elongation growth of petioles and hypocotyl. Thermomorphogenesis allows for optimal performance in suboptimal temperature conditions by enhancing the cooling capacity. When temperatures rise further, heat stress tolerance mechanisms can be induced that enable the plant to survive the stressful temperature, which typically comprises cellular protection mechanisms and memory thereof. Induction of thermomorphogenesis, heat stress tolerance and stress memory depend on gene expression regulation, governed by diverse epigenetic processes. In this Tansley review we update on the current knowledge of epigenetic regulation of heat stress tolerance and elevated temperature signalling and response, with a focus on thermomorphogenesis regulation and heat stress memory. In particular we highlight the emerging role of H3K4 methylation marks in diverse temperature signalling pathways.},
  language  = {en}
}
@article{FujiharaZhangJacksonetal.2022,
  author    = {Fujihara, Kenji M. and Zhang, Bonnie Z. and Jackson, Thomas D. and Ogunkola, Moses and Nijagal, Brunda and Milne, Julia V. and Sallman, David A. and Ang, Ching-Seng and Nikolic, Iva and Kearney, Conor J. and Hogg, Simon J. and Cabalag, Carlos S. and Sutton, Vivien R. and Watt, Sally and Fujihara, Asuka T. and Trapani, Joseph A. and Simpson, Kaylene J. and Stojanovski, Diana and Leimk{\"u}hler, Silke and Haupt, Sue and Phillips, Wayne A. and Clemons, Nicholas J.},
  title     = {Eprenetapopt triggers ferroptosis, inhibits NFS1 cysteine desulfurase, and synergizes with serine and glycine dietary restriction},
  series = {Science Advances},
  volume    = {8},
  journal   = {Science Advances},
  number    = {37},
  publisher = {American Assoc. for the Advancement of Science},
  address   = {Washington},
  issn      = {2375-2548},
  doi       = {10.1126/sciadv.abm9427},
  pages     = {13},
  year      = {2022},
  abstract  = {The mechanism of action of eprenetapopt (APR-246, PRIMA-1MET) as an anticancer agent remains unresolved, al-though the clinical development of eprenetapopt focuses on its reported mechanism of action as a mutant-p53 reactivator. Using unbiased approaches, this study demonstrates that eprenetapopt depletes cellular antioxidant glutathione levels by increasing its turnover, triggering a nonapoptotic, iron-dependent form of cell death known as ferroptosis. Deficiency in genes responsible for supplying cancer cells with the substrates for de novo glutathione synthesis (SLC7A11, SHMT2, and MTHFD1L), as well as the enzymes required to synthesize glutathione (GCLC and GCLM), augments the activity of eprenetapopt. Eprenetapopt also inhibits iron-sulfur cluster biogenesis by limit-ing the cysteine desulfurase activity of NFS1, which potentiates ferroptosis and may restrict cellular proliferation. The combination of eprenetapopt with dietary serine and glycine restriction synergizes to inhibit esophageal xenograft tumor growth. These findings reframe the canonical view of eprenetapopt from a mutant-p53 reactivator to a ferroptosis inducer.},
  language  = {en}
}
@article{GhafarianWielandNendel2022,
  author    = {Ghafarian, Fatemeh and Wieland, Ralf and Nendel, Claas},
  title     = {Estimating the Evaporative Cooling Effect of Irrigation within and above Soybean Canopy},
  series = {Water},
  volume    = {14},
  journal   = {Water},
  number    = {3},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2073-4441},
  doi       = {10.3390/w14030319},
  pages     = {16},
  year      = {2022},
  abstract  = {Vegetation with an adequate supply of water might contribute to cooling the land surface around it through the latent heat flux of transpiration. This study investigates the potential estimation of evaporative cooling at plot scale, using soybean as example. Some of the plants' physiological parameters were monitored and sampled at weekly intervals. A physics-based model was then applied to estimate the irrigation-induced cooling effect within and above the canopy during the middle and late season of the soybean growth period. We then examined the results of the temperature changes at a temporal resolution of ten minutes between every two irrigation rounds. During the middle and late season of growth, the cooling effects caused by evapotranspiration within and above the canopy were, on average, 4.4 K and 2.9 K, respectively. We used quality indicators such as R-squared (R-2) and mean absolute error (MAE) to evaluate the performance of the model simulation. The performance of the model in this study was better above the canopy (R-2 = 0.98, MAE = 0.3 K) than below (R-2 = 0.87, MAE = 0.9 K) due to the predefined thermodynamic condition used to estimate evaporative cooling. Moreover, the study revealed that canopy cooling contributes to mitigating heat stress conditions during the middle and late seasons of crop growth.},
  language  = {en}
}
@article{HermanussenScheffler2022,
  author    = {Hermanussen, Michael and Scheffler, Christiane},
  title     = {Evidence of chronic undernutrition in late 19th century German infants of all social classes},
  series = {Human biology and public health},
  volume    = {2022},
  journal   = {Human biology and public health},
  number    = {2},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  issn      = {2748-9957},
  doi       = {10.52905/hbph2022.2.42},
  pages     = {9},
  year      = {2022},
  abstract  = {125 years ago, European infants grew differently from modern infants. We show weight gains of 20 healthy children weighed longitudinally from birth to age 1 year, published by Camerer in 1882. The data illustrate the historically prevalent concepts of infant nutrition practiced by German civil servants, lawyers, merchants, university professors, physicians, foresters and farmers. Breastfeeding by the mother was not truly appreciated in those days; children were often breastfed by wet nurses or received bottled milk. Bottle feeding mainly used diluted cow's milk with some added carbohydrates, without evidence that appropriate amounts of oil, butter or other fatty components were added. French children from 1914 showed similar weight gain patterns suggesting similar feeding practices. The historical data suggest that energy deficient infant formula was fed regularly in the late 19th and early 20th century Europe, regardless of wealth and social class. The data question current concerns that temporarily feeding energy deficient infant formula may warrant serious anxieties regarding long-term cognitive, social and emotional behavioral development.},
  language  = {en}
}
@article{EhrlichThygesenKiorboe2022,
  author    = {Ehrlich, Elias and Thygesen, Uffe H{\o}gsbro and Ki{\o}rboe, Thomas},
  title     = {Evolution of toxins as a public good in phytoplankton},
  series = {Proceedings of the Royal Society of London : B, Biological sciences},
  volume    = {289},
  journal   = {Proceedings of the Royal Society of London : B, Biological sciences},
  number    = {1977},
  publisher = {Royal Society},
  address   = {London},
  issn      = {0962-8452},
  doi       = {10.1098/rspb.2022.0393},
  pages     = {9},
  year      = {2022},
  abstract  = {Toxic phytoplankton blooms have increased in many waterbodies worldwide with well-known negative impacts on human health, fisheries and ecosystems. However, why and how phytoplankton evolved toxin production is still a puzzling question, given that the producer that pays the costs often shares the benefit with other competing algae and thus provides toxins as a 'public good' (e.g. damaging a common competitor or predator). Furthermore, blooming phytoplankton species often show a high intraspecific variation in toxicity and we lack an understanding of what drives the dynamics of coexisting toxic and non-toxic genotypes. Here, by using an individual-based two-dimensional model, we show that small-scale patchiness of phytoplankton strains caused by demography can explain toxin evolution in phytoplankton with low motility and the maintenance of genetic diversity within their blooms. This patchiness vanishes for phytoplankton with high diffusive motility, suggesting different evolutionary pathways for different phytoplankton groups. In conclusion, our study reveals that small-scale spatial heterogeneity, generated by cell division and counteracted by diffusive cell motility and turbulence, can crucially affect toxin evolution and eco-evolutionary dynamics in toxic phytoplankton species. This contributes to a better understanding of conditions favouring toxin production and the evolution of public goods in asexually reproducing organisms in general.},
  language  = {en}
}
@article{HilgersHartmannPfaenderetal.2022,
  author    = {Hilgers, Leon and Hartmann, Stefanie and Pfaender, Jobst and Lentge-Maass, Nora and Marwoto, Ristiyanti M. and von Rintelen, Thomas and Hofreiter, Michael},
  title     = {Evolutionary divergence and radula diversification in two ecomorphs from an adaptive radiation of freshwater snails},
  series = {Genes},
  volume    = {13},
  journal   = {Genes},
  number    = {6},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2073-4425},
  doi       = {10.3390/genes13061029},
  pages     = {16},
  year      = {2022},
  abstract  = {(1) Background: Adaptive diversification of complex traits plays a pivotal role in the evolution of organismal diversity. In the freshwater snail genus Tylomelania, adaptive radiations were likely promoted by trophic specialization via diversification of their key foraging organ, the radula. (2) Methods: To investigate the molecular basis of radula diversification and its contribution to lineage divergence, we used tissue-specific transcriptomes of two sympatric Tylomelania sarasinorum ecomorphs. (3) Results: We show that ecomorphs are genetically divergent lineages with habitat-correlated abundances. Sequence divergence and the proportion of highly differentially expressed genes are significantly higher between radula transcriptomes compared to the mantle and foot. However, the same is not true when all differentially expressed genes or only non-synonymous SNPs are considered. Finally, putative homologs of some candidate genes for radula diversification (hh, arx, gbb) were also found to contribute to trophic specialization in cichlids and Darwin's finches. (4) Conclusions: Our results are in line with diversifying selection on the radula driving Tylomelania ecomorph divergence and indicate that some molecular pathways may be especially prone to adaptive diversification, even across phylogenetically distant animal groups.},
  language  = {en}
}
@article{PohankovaHlavinkaKersebaumetal.2022,
  author    = {Pohankov{\´a}, Eva and Hlavinka, Petr and Kersebaum, Kurt-Christian and Rodr{\´i}guez, Alfredo and Balek, Jan and Bednař{\´i}k, Martin and Dubrovsk{\´y}, Martin and Gobin, Anne and Hoogenboom, Gerrit and Moriondo, Marco and Nendel, Claas and Olesen, J{\o}rgen E. E. and R{\"o}tter, Reimund Paul and Ruiz-Ramos, Margarita and Shelia, Vakhtang and Stella, Tommaso and Hoffmann, Munir Paul and Tak{\´a}č, Jozef and Eitzinger, Josef and Dibari, Camilla and Ferrise, Roberto and Bl{\´a}hov{\´a}, Monika and Trnka, Miroslav},
  title     = {Expected effects of climate change on the production and water use of crop rotation management reproduced by crop model ensemble for Czech Republic sites},
  series = {European journal of agronomy},
  volume    = {134},
  journal   = {European journal of agronomy},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1161-0301},
  doi       = {10.1016/j.eja.2021.126446},
  pages     = {27},
  year      = {2022},
  abstract  = {Crop rotation, fertilization and residue management affect the water balance and crop production and can lead to different sensitivities to climate change. To assess the impacts of climate change on crop rotations (CRs), the crop model ensemble (APSIM,AQUACROP, CROPSYST, DAISY, DSSAT, HERMES, MONICA) was used. The yields and water balance of two CRs with the same set of crops (winter wheat, silage maize, spring barley and winter rape) in a continuous transient run from 1961 to 2080 were simulated. CR1 was without cover crops and without manure application. Straw after the harvest was exported from the fields. CR2 included cover crops, manure application and crop residue retention left on field. Simulations were performed using two soil types (Chernozem, Cambisol) within three sites in the Czech Republic, which represent temperature and precipitation gradients for crops in Central Europe. For the description of future climatic conditions, seven climate scenarios were used. Six of them had increasing CO \& nbsp;concentrations according RCP 8.5, one had no CO2 increase in the future. The output of an ensemble expected higher productivity by 0.82 t/ha/year and 2.04 t/ha/year for yields and aboveground biomass in the future (2051-2080). However, if the direct effect of a CO2 increase is not considered, the average yields for lowlands will be lower. Compared to CR1, CR2 showed higher average yields of 1.26 t/ha/year for current climatic conditions and 1.41 t/ha/year for future climatic conditions. For the majority of climate change scenarios, the crop model ensemble agrees on the projected yield increase in C3 crops in the future for CR2 but not for CR1. Higher agreement for future yield increases was found for Chernozem, while for Cambisol, lower yields under dry climate scenarios are expected. For silage maize, changes in simulated yields depend on locality. If the same hybrid will be used in the future, then yield reductions should be expected within lower altitudes. The results indicate the potential for higher biomass production from cover crops, but CR2 is associated with almost 120 mm higher evapotranspiration compared to that of CR1 over a 5-year cycle for lowland stations in the future, which in the case of the rainfed agriculture could affect the long-term soil water balance. This could affect groundwater replenishment, especially for locations with fine textured soils, although the findings of this study highlight the potential for the soil water-holding capacity to buffer against the adverse weather conditions.},
  language  = {en}
}
@article{MalacrinoAbdelfattahBergetal.2022,
  author    = {Malacrin{\`o}, Antonino and Abdelfattah, Ahmed and Berg, Gabriele and Benitez, Maria-Soledad and Bennett, Alison E. and B{\"o}ttner, Laura and Xu, Shuqing and Schena, Leonardo},
  title     = {Exploring microbiomes for plant disease management},
  series = {Biological control : theory and application in pest management},
  volume    = {169},
  journal   = {Biological control : theory and application in pest management},
  publisher = {Academic Press},
  address   = {San Diego, Calif.},
  issn      = {1049-9644},
  doi       = {10.1016/j.biocontrol.2022.104890},
  pages     = {7},
  year      = {2022},
  abstract  = {Microbiome science is revolutionizing many concepts of plant biology, ecology, and evolution. Understanding plant microbiomes is key to developing solutions that protect crop health without impacting the environment. In this perspective article, we highlight the importance of both the structure and functions of plant-associated microbial communities in protecting their host from pathogens. These new findings have a high potential to aid biocontrol programs and to replace traditional chemical products, guiding the transition towards a sustainable production.},
  language  = {en}
}
@article{HagbergCeleminIrisarrietal.2022,
  author    = {Hagberg, Linda and Celemin, Enrique and Irisarri, Iker and Hawlitschek, Oliver and Bella, Jose L. and Mott, Tami and Pereira, Ricardo J.},
  title     = {Extensive introgression at late stages of species formation},
  series = {Molecular ecology},
  volume    = {31},
  journal   = {Molecular ecology},
  number    = {8},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0962-1083},
  doi       = {10.1111/mec.16406},
  pages     = {2384 -- 2399},
  year      = {2022},
  abstract  = {The process of species formation is characterized by the accumulation of multiple reproductive barriers. The evolution of hybrid male sterility, or Haldane's rule, typically characterizes later stages of species formation, when reproductive isolation is strongest. Yet, understanding how quickly reproductive barriers evolve and their consequences for maintaining genetic boundaries between emerging species remains a challenging task because it requires studying taxa that hybridize in nature. Here, we address these questions using the meadow grasshopper Pseudochorthippus parallelus, where populations that show multiple reproductive barriers, including hybrid male sterility, hybridize in two natural hybrid zones. Using mitochondrial data, we infer that such populations diverged some 100,000 years ago, at the beginning of the last glacial cycle in Europe. Nuclear data show that contractions at multiple glacial refugia, and post-glacial expansions have facilitated genetic differentiation between lineages that today interact in hybrid zones. We find extensive introgression throughout the sampled species range, irrespective of the current strength of reproductive isolation. Populations exhibiting hybrid male sterility in two hybrid zones show repeatable patterns of genomic differentiation, consistent with shared genomic constraints affecting ancestral divergence or with the role of those regions in reproductive isolation. Together, our results suggest that reproductive barriers that characterize late stages of species formation can evolve relatively quickly, particularly when associated with strong demographic changes. Moreover, we show that such barriers persist in the face of extensive gene flow, allowing future studies to identify associated genomic regions.},
  language  = {en}
}
@article{RistowPanitsaMeyeretal.2022,
  author    = {Ristow, Michael and Panitsa, Maria and Meyer, Stefan and Bergmeier, Erwin},
  title     = {Factors of detection deficits in vascular plant inventories - an island case study},
  series = {Diversity},
  volume    = {14},
  journal   = {Diversity},
  number    = {4},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {1424-2818},
  doi       = {10.3390/d14040303},
  pages     = {12},
  year      = {2022},
  abstract  = {The degree of completeness of large-scale floristic inventories is often difficult to judge. We compared prior vascular plant species inventories of the Mediterranean island of Limnos (North Aegean, Greece) with 231 recent records from 2016-2021. Together with the recent records, the known number of vascular plant species on the island is 960 native taxa, 63 established neophytes, and 27 species of as yet casual status for a total of 1050 taxa. We looked at a number of traits (plant family, size, flower color, perceptibility, habitat, reproduction period, rarity, and status) to investigate whether they were overrepresented in the dataset of the newly found taxa. Overrepresentation was found in some plant families (e.g., Poaceae and Chenopodiaceae) and for traits such as hydrophytic life form, unobtrusive flower color, coastal as well as agricultural and ruderal habitats, and late (summer/autumn) reproduction period. Apart from the well-known fact of esthetic bias, we found evidence for ecological and perceptibility biases. Plant species inventories based on prior piecemeal collated data should focus on regionally specific species groups and underrepresented and rare habitats.},
  language  = {en}
}
@article{JaenickeGoddardSteinetal.2022,
  author    = {J{\"a}nicke, Clemens and Goddard, Adam and Stein, Susanne and Steinmann, Horst-Henning and Lakes, Tobia and Nendel, Claas and M{\"u}ller, Daniel},
  title     = {Field-level land-use data reveal heterogeneous crop sequences with distinct regional differences in Germany},
  series = {European journal of agronomy},
  volume    = {141},
  journal   = {European journal of agronomy},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1161-0301},
  doi       = {10.1016/j.eja.2022.126632},
  pages     = {12},
  year      = {2022},
  abstract  = {Crop cultivation intensifies globally, which can jeopardize biodiversity and the resilience of cropping systems. We investigate changes in crop rotations as one intensification metric for half of the croplands in Germany with annual field-level land-use data from 2005 to 2018. We proxy crop rotations with crop sequences and compare how these sequences changed among three seven-year periods. The results reveal an overall high diversity of crop sequences in Germany. Half of the cropland has crop sequences with four or more crops within a seven-year period, while continuous cultivation of the same crop is present on only 2\% of the cropland. Larger farms tend to have more diverse crop sequences and organic farms have lower shares of cereal crops. In three federal states, crop rotations became less structurally diverse over time, i.e. the number of crops and the number of changes between crops decreased. In one state, structural diversity increased and the proportion of monocropping decreased. The functional diversity of the crop sequences, which measures the share of winter and spring crops as well as the share of leaf and cereal crops per sequence, remained largely stable. Trends towards cereal-or leaf -crop dominated sequences varied between the states, and no clear overall dynamic could be observed. However, the share of winter crops per sequence decreased in all four federal states. Quantifying the dynamics of crop sequences at the field level is an important metric of land-use intensity and can reveal the patterns of land-use intensification.},
  language  = {en}
}
@article{WeyrichGuerreroAltamiranoYasaretal.2022,
  author    = {Weyrich, Alexandra and Guerrero-Altamirano, Tania P. and Yasar, Selma and Czirjak, G{\´a}bor-{\´A}rp{\´a}d and Wachter, Bettina and Fickel, J{\"o}rns},
  title     = {First Steps towards the development of epigenetic biomarkers in female cheetahs (Acinonyx jubatus)},
  series = {Life : open access journal},
  volume    = {12},
  journal   = {Life : open access journal},
  number    = {6},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2075-1729},
  doi       = {10.3390/life12060920},
  pages     = {16},
  year      = {2022},
  abstract  = {Free-ranging cheetahs (Acinonyx jubatus) are generally healthy, whereas cheetahs under human care, such as those in zoological gardens, suffer from ill-defined infectious and degenerative pathologies. These differences are only partially explained by husbandry management programs because both groups share low genetic diversity. However, mounting evidence suggests that physiological differences between populations in different environments can be tracked down to differences in epigenetic signatures. Here, we identified differentially methylated regions (DMRs) between free-ranging cheetahs and conspecifics in zoological gardens and prospect putative links to pathways relevant to immunity, energy balance and homeostasis. Comparing epigenomic DNA methylation profiles obtained from peripheral blood mononuclear cells (PBMCs) from eight free-ranging female cheetahs from Namibia and seven female cheetahs living in zoological gardens within Europe, we identified DMRs of which 22 were hypermethylated and 23 hypomethylated. Hypermethylated regions in cheetahs under human care were located in the promoter region of a gene involved in host-pathogen interactions (KLC1) and in an intron of a transcription factor relevant for the development of pancreatic beta-cells, liver, and kidney (GLIS3). The most canonical mechanism of DNA methylation in promoter regions is assumed to repress gene transcription. Taken together, this could indicate that hypermethylation at the promoter region of KLC1 is involved in the reduced immunity in cheetahs under human care. This approach can be generalized to characterize DNA methylation profiles in larger cheetah populations under human care with a more granular longitudinal data collection, which, in the future, could be used to monitor the early onset of pathologies, and ultimately translate into the development of biomarkers with prophylactic and/or therapeutic potential.},
  language  = {en}
}
@article{EccardHerdeSchusteretal.2022,
  author    = {Eccard, Jana and Herde, Antje and Schuster, Andrea C. and Liesenjohann, Thilo and Knopp, Tatjana and Heckel, Gerald and Dammhahn, Melanie},
  title     = {Fitness, risk taking, and spatial behavior covary with boldness in experimental vole populations},
  series = {Ecology And Evolution},
  journal   = {Ecology And Evolution},
  publisher = {John Wiley \& Sons, Inc.},
  address   = {Vereinigte Staaten},
  issn      = {2045-7758},
  doi       = {10.1002/ece3.8521},
  pages     = {1 -- 15},
  year      = {2022},
  abstract  = {Individuals of a population may vary along a pace-of-life syndrome from highly fecund, short-lived, bold, dispersive "fast" types at one end of the spectrum to less fecund, long-lived, shy, plastic "slow" types at the other end. Risk-taking behavior might mediate the underlying life history trade-off, but empirical evidence supporting this hypothesis is still ambiguous. Using experimentally created populations of common voles (Microtus arvalis)—a species with distinct seasonal life history trajectories—we aimed to test whether individual differences in boldness behavior covary with risk taking, space use, and fitness. We quantified risk taking, space use (via automated tracking), survival, and reproductive success (via genetic parentage analysis) in 8 to 14 experimental, mixed-sex populations of 113 common voles of known boldness type in large grassland enclosures over a significant part of their adult life span and two reproductive events. Populations were assorted to contain extreme boldness types (bold or shy) of both sexes. Bolder individuals took more risks than shyer ones, which did not affect survival. Bolder males but not females produced more offspring than shy conspecifics. Daily home range and core area sizes, based on 95\% and 50\% Kernel density estimates (20 ± 10 per individual, n = 54 individuals), were highly repeatable over time. Individual space use unfolded differently for sex-boldness type combinations over the course of the experiment. While day ranges decreased for shy females, they increased for bold females and all males. Space use trajectories may, hence, indicate differences in coping styles when confronted with a novel social and physical environment. Thus, interindividual differences in boldness predict risk taking under near-natural conditions and have consequences for fitness in males, which have a higher reproductive potential than females. Given extreme inter- and intra-annual fluctuations in population density in the study species and its short life span, density-dependent fluctuating selection operating differently on the sexes might maintain (co)variation in boldness, risk taking, and pace-of-life.},
  language  = {en}
}
@article{VillalbaKarnatakGrossartetal.2022,
  author    = {Villalba, Luis Alberto and Karnatak, Rajat and Grossart, Hans-Peter and Wollrab, Sabine},
  title     = {Flexible habitat choice of pelagic bacteria increases system stability and energy flow through the microbial loop},
  series = {Limnology and oceanography : L \& O},
  volume    = {67},
  journal   = {Limnology and oceanography : L \& O},
  number    = {6},
  publisher = {Wiley-Blackwell},
  address   = {Oxford [u.a.]},
  issn      = {0024-3590},
  doi       = {10.1002/lno.12091},
  pages     = {1402 -- 1415},
  year      = {2022},
  abstract  = {Pelagic bacteria can be classified into free-living and particle-attached life modes, which either dwell in the water column or attach to suspended particles. Bacteria with a generalist life style, however, can actively shift between these two habitats. Globally increasing densities of natural and artificial particles enhance habitat heterogeneity, with potential consequences for system stability and trophic transfer through aquatic food webs. To better decipher the dynamics of microbial communities, we investigated the influence of adaptive vs. fixed habitat choice on species coexistence for a simplified bacterial community by analyzing a corresponding food web model, consisting of two specialist bacterial prey species (free and attached), a generalist bacterial prey species with the ability to shift between both habitats, and two protist predators, specialized on either water or particle compartment. For simplicity we assume a shared resource pool, considering particles only for colonization but not as a source for nutrients or carbon, that is, inert particles like microplastics or inorganic sediments. The model predicts coexistence on a cyclic attractor between fixed and flexible bacteria, if the costs for adaptive habitat choice can be balanced by adaptation speed. The presence of adaptive prey dampens predator-prey cycle amplitudes, contributing to system stabilization resulting in higher mean predator biomass compared to specialist prey only. Thus, in pelagic microbial systems, flexible habitat choice at the prey level has important implications for system stability and magnitude of energy flow through the microbial loop.},
  language  = {en}
}
@article{MillesDammhahnJeltschetal.2022,
  author    = {Milles, Alexander Benedikt and Dammhahn, Melanie and Jeltsch, Florian and Schl{\"a}gel, Ulrike and Grimm, Volker},
  title     = {Fluctuations in density-dependent selection drive the evolution of a pace-of-life syndrome within and between populations},
  series = {The American naturalist : a bi-monthly journal devoted to the advancement and correlation of the biological sciences},
  volume    = {199},
  journal   = {The American naturalist : a bi-monthly journal devoted to the advancement and correlation of the biological sciences},
  number    = {4},
  publisher = {Univ. of Chicago Press},
  address   = {Chicago},
  issn      = {0003-0147},
  doi       = {10.1086/718473},
  pages     = {E124 -- E139},
  year      = {2022},
  abstract  = {The pace-of-life syndrome (POLS) hypothesis posits that suites of traits are correlated along a slow-fast continuum owing to life history trade-offs. Despite widespread adoption, environmental conditions driving the emergence of POLS remain unclear. A recently proposed conceptual framework of POLS suggests that a slow-fast continuum should align to fluctuations in density-dependent selection. We tested three key predictions made by this framework with an ecoevolutionary agent-based population model. Selection acted on responsiveness (behavioral trait) to interpatch resource differences and the reproductive investment threshold (life history trait). Across environments with density fluctuations of different magnitudes, we observed the emergence of a common axis of trait covariation between and within populations (i.e., the evolution of a POLS). Slow-type (fast-type) populations with high (low) responsiveness and low (high) reproductive investment threshold were selected at high (low) population densities and less (more) intense and frequent density fluctuations. In support of the predictions, fast-type populations contained a higher degree of variation in traits and were associated with higher intrinsic reproductive rate (r(0)) and higher sensitivity to intraspecific competition (gamma), pointing to a universal trade-off. While our findings support that POLS aligns with density-dependent selection, we discuss possible mechanisms that may lead to alternative evolutionary pathways.},
  language  = {en}
}
@article{MendesFerreiraDammhahnEccard2022,
  author    = {Mendes Ferreira, Clara and Dammhahn, Melanie and Eccard, Jana},
  title     = {Forager-mediated cascading effects on food resource species diversity},
  series = {Ecology and Evolution},
  volume    = {12},
  journal   = {Ecology and Evolution},
  number    = {11},
  publisher = {John Wiley \& Sons},
  issn      = {2045-7758},
  doi       = {10.1002/ece3.9523},
  pages     = {13},
  year      = {2022},
  abstract  = {Perceived predation risk varies in space and time. Foraging in this landscape of fear alters forager-resource interactions via cascading nonconsumptive effects. Estimating these indirect effects is difficult in natural systems. Here, we applied a novel measure to quantify the diversity at giving-up density that allows to test how spatial variation in perceived predation risk modifies the diversity of multispecies resources at local and regional spatial levels. Furthermore, we evaluated whether the nonconsumptive effects on resource species diversity can be explained by the preferences of foragers for specific functional traits and by the forager species richness. We exposed rodents of a natural community to artificial food patches, each containing an initial multispecies resource community of eight species (10 items each) mixed in sand. We sampled 35 landscapes, each containing seven patches in a spatial array, to disentangle effects at local (patch) and landscape levels. We used vegetation height as a proxy for perceived predation risk. After a period of three nights, we counted how many and which resource species were left in each patch to measure giving-up density and resource diversity at the local level (alpha diversity) and the regional level (gamma diversity and beta diversity). Furthermore, we used wildlife cameras to identify foragers and assess their species richness. With increasing vegetation height, i.e., decreasing perceived predation risk, giving-up density, and local alpha and regional gamma diversity decreased, and patches became less similar within a landscape (beta diversity increased). Foragers consumed more of the bigger and most caloric resources. The higher the forager species richness, the lower the giving-up density, and alpha and gamma diversity. Overall, spatial variation of perceived predation risk of foragers had measurable cascading effects on local and regional resource species biodiversity, independent of the forager species. Thus, nonconsumptive predation effects modify forager-resource interactions and might act as an equalizing mechanism for species coexistence.},
  language  = {en}
}
@article{SchulteLiLisovskietal.2022,
  author    = {Schulte, Luise and Li, Chenzhi and Lisovski, Simeon and Herzschuh, Ulrike},
  title     = {Forest-permafrost feedbacks and glacial refugia help explain the unequal distribution of larch across continents},
  series = {Journal of biogeography},
  volume    = {49},
  journal   = {Journal of biogeography},
  number    = {10},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0305-0270},
  doi       = {10.1111/jbi.14456},
  pages     = {1825 -- 1838},
  year      = {2022},
  abstract  = {Aim: The continental-scale distribution of plant functional types, such as evergreen and summergreen needle-leaf forest, is assumed to be determined by contemporary climate. However, the distribution of summergreen needle-leaf forest of larch (Larix Mill.) differs markedly between the continents, despite relatively similar climatic conditions. The reasons for these differences are little understood. Our aim is to identify potential triggers and drivers of the current distribution patterns by comparing species' bioclimatic niches, glacial refugia and postglacial recolonization patterns. Location: Northern hemisphere. Taxon: Species of the genus Larix (Mill.). Methods: We compare species distribution and dominance using species ranges and sites of dominance, as well as their occurrence on modelled permafrost extent, and active layer thickness (ALT). We compare the bioclimatic niches and calculate the niche overlap between species, using the same data in addition to modern climate data. We synthesize pollen, macrofossil and ancient DNA palaeo-evidence of past Larix occurrences of the last 60,000 years and track differences in distribution patterns through time. Results: Bioclimatic niches show large overlaps between Asian larch species and American Larix laricina. The distribution across various degrees of permafrost extent is distinctly different for Asian L. gmelinii and L. cajanderi compared to the other species, whereas the distribution on different depths of ALT is more similar among Asian and American species. Northern glacial refugia for Larix are only present in eastern Asia and Alaska. Main Conclusion: The dominance of summergreen larches in Asia, where evergreen conifers dominate most of the rest of the boreal forests, is dependent on the interaction of several factors which allows Asian L. gmelinii and L. cajanderi to dominate where these factors coincide. These factors include the early postglacial spread out of northern glacial refugia in the absence of competitors as well as a positive feedback mechanism between frozen ground and forest.},
  language  = {en}
}
@article{IonescuBizicKarnataketal.2022,
  author    = {Ionescu, Danny and Bizic, Mina and Karnatak, Rajat and Musseau, Camille L. and Onandia, Gabriela and Kasada, Minoru and Berger, Stella A. and Nejstgaard, Jens Christian and Ryo, Masahiro and Lischeid, Gunnar and Gessner, Mark O. and Wollrab, Sabine and Grossart, Hans-Peter},
  title     = {From microbes to mammals: Pond biodiversity homogenization across different land-use types in an agricultural landscape},
  series = {Ecological monographs},
  volume    = {92},
  journal   = {Ecological monographs},
  number    = {3},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0012-9615},
  doi       = {10.1002/ecm.1523},
  pages     = {28},
  year      = {2022},
  abstract  = {Local biodiversity patterns are expected to strongly reflect variation in topography, land use, dispersal boundaries, nutrient supplies, contaminant spread, management practices, and other anthropogenic influences. Contrary to this expectation, studies focusing on specific taxa revealed a biodiversity homogenization effect in areas subjected to long-term intensive industrial agriculture. We investigated whether land use affects biodiversity levels and community composition (alpha- and beta-diversity) in 67 kettle holes (KH) representing small aquatic islands embedded in the patchwork matrix of a largely agricultural landscape comprising grassland, forest, and arable fields. These KH, similar to millions of standing water bodies of glacial origin, spread across northern Europe, Asia, and North America, are physico-chemically diverse and differ in the degree of coupling with their surroundings. We assessed aquatic and sediment biodiversity patterns of eukaryotes, Bacteria, and Archaea in relation to environmental features of the KH, using deep-amplicon-sequencing of environmental DNA (eDNA). First, we asked whether deep sequencing of eDNA provides a representative picture of KH aquatic biodiversity across the Bacteria, Archaea, and eukaryotes. Second, we investigated if and to what extent KH biodiversity is influenced by the surrounding land use. We hypothesized that richness and community composition will greatly differ in KH from agricultural land use compared with KH in grasslands and forests. Our data show that deep eDNA amplicon sequencing is useful for in-depth assessments of cross-domain biodiversity comprising both micro- and macro-organisms, but has limitations with respect to single-taxa conservation studies. Using this broad method, we show that sediment eDNA, integrating several years to decades, depicts the history of agricultural land-use intensification. Aquatic biodiversity was best explained by seasonality, whereas land-use type explained little of the variation. We concluded that, counter to our hypothesis, land use intensification coupled with landscape wide nutrient enrichment (including atmospheric deposition), groundwater connectivity between KH and organismal (active and passive) dispersal in the tight network of ponds, resulted in a biodiversity homogenization in the KH water, leveling off today's detectable differences in KH biodiversity between land-use types. These findings have profound implications for measures and management strategies to combat current biodiversity loss in agricultural landscapes worldwide.},
  language  = {en}
}
@article{SporbertJakubkaBucheretal.2022,
  author    = {Sporbert, Maria and Jakubka, Desiree and Bucher, Solveig Franziska and Hensen, Isabell and Freiberg, Martin and Heubach, Katja and K{\"o}nig, Andreas and Nordt, Birgit and Plos, Carolin and Blinova, Ilona and Bonn, Aletta and Knickmann, Barbara and Koubek, Tom{\´a}š and Linst{\"a}dter, Anja and Maškov{\´a}, Tereza and Primack, Richard B. and Rosche, Christoph and Shah, Manzoor A. and Stevens, Albert-Dieter and Tielb{\"o}rger, Katja and Tr{\"a}ger, Sabrina and Wirth, Christian and R{\"o}mermann, Christine},
  title     = {Functional traits influence patterns in vegetative and reproductive plant phenology - a multi-botanical garden study},
  series = {New phytologist},
  volume    = {235},
  journal   = {New phytologist},
  number    = {6},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0028-646X},
  doi       = {10.1111/nph.18345},
  pages     = {2199 -- 2210},
  year      = {2022},
  abstract  = {Phenology has emerged as key indicator of the biological impacts of climate change, yet the role of functional traits constraining variation in herbaceous species' phenology has received little attention. Botanical gardens are ideal places in which to investigate large numbers of species growing under common climate conditions. We ask whether interspecific variation in plant phenology is influenced by differences in functional traits. We recorded onset, end, duration and intensity of initial growth, leafing out, leaf senescence, flowering and fruiting for 212 species across five botanical gardens in Germany. We measured functional traits, including plant height, absolute and specific leaf area, leaf dry matter content, leaf carbon and nitrogen content and seed mass and accounted for species' relatedness. Closely related species showed greater similarities in timing of phenological events than expected by chance, but species' traits had a high degree of explanatory power, pointing to paramount importance of species' life-history strategies. Taller plants showed later timing of initial growth, and flowered, fruited and underwent leaf senescence later. Large-leaved species had shorter flowering and fruiting durations. Taller, large-leaved species differ in their phenology and are more competitive than smaller, small-leaved species. We assume climate warming will change plant communities' competitive hierarchies with consequences for biodiversity.},
  language  = {en}
}
@article{HagemannConejeroStillfriedetal.2022,
  author    = {Hagemann, Justus and Conejero, Carles and Stillfried, Milena and Mentaberre, Gregorio and Castillo-Contreras, Raquel and Fickel, J{\"o}rns and Lopez-Olvera, Jorge Ram{\´o}n},
  title     = {Genetic population structure defines wild boar as an urban exploiter species in Barcelona, Spain},
  series = {The science of the total environment : an international journal for scientific research into the environment and its relationship with man},
  volume    = {833},
  journal   = {The science of the total environment : an international journal for scientific research into the environment and its relationship with man},
  publisher = {Elsevier Science},
  address   = {Amsterdam [u.a.]},
  issn      = {0048-9697},
  doi       = {10.1016/j.scitotenv.2022.155126},
  pages     = {10},
  year      = {2022},
  abstract  = {Urban wildlife ecology is gaining relevance as metropolitan areas grow throughout the world, reducing natural habitats and creating new ecological niches. However, knowledge is still scarce about the colonisation processes of such urban niches, the establishment of new communities, populations and/or species, and the related changes in behaviour and life histories of urban wildlife. Wild boar (Sus scrofa) has successfully colonised urban niches throughout Europe. The aim of this study is to unveil the processes driving the establishment and maintenance of an urban wild boar population by analysing its genetic structure. A set of 19 microsatellite loci was used to test whether urban wild boars in Barcelona, Spain, are an isolated population or if gene flow prevents genetic differentiation between rural and urban wild boars. This knowledge will contribute to the understanding of the effects of synurbisation and the associated management measures on the genetic change of large mammals in urban ecosystems. Despite the unidirectional gene flow from rural to urban areas, the urban wild boars in Barcelona form an island population genotypically differentiated from the surrounding rural ones. The comparison with previous genetic studies of urban wild boar populations suggests that forest patches act as suitable islands for wild boar genetic differentiation. Previous results and the genetic structure of the urban wild boar population in Barcelona classify wild boar as an urban exploiter species. These wild boar peri-urban island populations are responsible for conflict with humans and thus should be managed by reducing the attractiveness of urban areas. The management of peri-urban wild boar populations should aim at reducing migration into urban areas and preventing phenotypic changes (either genetic or plastic) causing habituation of wild boars to humans and urban environments.},
  language  = {en}
}
@article{PalmaVeraReyerLanghammeretal.2022,
  author    = {Palma-Vera, Sergio E. and Reyer, Henry and Langhammer, Martina and Reinsch, Norbert and Derezanin, Lorena and Fickel, J{\"o}rns and Qanbari, Saber and Weitzel, Joachim M. and Franzenburg, Soeren and Hemmrich-Stanisak, Georg and Sch{\"o}n, Jennifer},
  title     = {Genomic characterization of the world's longest selection experiment in mouse reveals the complexity of polygenic traits},
  series = {BMC Biology},
  volume    = {20},
  journal   = {BMC Biology},
  number    = {1},
  publisher = {BMC},
  address   = {London},
  issn      = {1741-7007},
  doi       = {10.1186/s12915-022-01248-9},
  pages     = {20},
  year      = {2022},
  abstract  = {Background Long-term selection experiments are a powerful tool to understand the genetic background of complex traits. The longest of such experiments has been conducted in the Research Institute for Farm Animal Biology (FBN), generating extreme mouse lines with increased fertility, body mass, protein mass and endurance. For >140 generations, these lines have been maintained alongside an unselected control line, representing a valuable resource for understanding the genetic basis of polygenic traits. However, their history and genomes have not been reported in a comprehensive manner yet. Therefore, the aim of this study is to provide a summary of the breeding history and phenotypic traits of these lines along with their genomic characteristics. We further attempt to decipher the effects of the observed line-specific patterns of genetic variation on each of the selected traits. Results Over the course of >140 generations, selection on the control line has given rise to two extremely fertile lines (>20 pups per litter each), two giant growth lines (one lean, one obese) and one long-distance running line. Whole genome sequencing analysis on 25 animals per line revealed line-specific patterns of genetic variation among lines, as well as high levels of homozygosity within lines. This high degree of distinctiveness results from the combined effects of long-term continuous selection, genetic drift, population bottleneck and isolation. Detection of line-specific patterns of genetic differentiation and structural variation revealed multiple candidate genes behind the improvement of the selected traits. Conclusions The genomes of the Dummerstorf trait-selected mouse lines display distinct patterns of genomic variation harbouring multiple trait-relevant genes. Low levels of within-line genetic diversity indicate that many of the beneficial alleles have arrived to fixation alongside with neutral alleles. This study represents the first step in deciphering the influence of selection and neutral evolutionary forces on the genomes of these extreme mouse lines and depicts the genetic complexity underlying polygenic traits.},
  language  = {en}
}
@article{TongNankarLiuetal.2022,
  author    = {Tong, Hao and Nankar, Amol N. and Liu, Jintao and Todorova, Velichka and Ganeva, Daniela and Grozeva, Stanislava and Tringovska, Ivanka and Pasev, Gancho and Radeva-Ivanova, Vesela and Gechev, Tsanko and Kostova, Dimitrina and Nikoloski, Zoran},
  title     = {Genomic prediction of morphometric and colorimetric traits in Solanaceous fruits},
  series = {Horticulture research},
  volume    = {9},
  journal   = {Horticulture research},
  publisher = {Oxford Univ. Press},
  address   = {Cary},
  issn      = {2052-7276},
  doi       = {10.1093/hr/uhac072},
  pages     = {11},
  year      = {2022},
  abstract  = {Selection of high-performance lines with respect to traits of interest is a key step in plant breeding. Genomic prediction allows to determine the genomic estimated breeding values of unseen lines for trait of interest using genetic markers, e.g. single-nucleotide polymorphisms (SNPs), and machine learning approaches, which can therefore shorten breeding cycles, referring to genomic selection (GS). Here, we applied GS approaches in two populations of Solanaceous crops, i.e. tomato and pepper, to predict morphometric and colorimetric traits. The traits were measured by using scoring-based conventional descriptors (CDs) as well as by Tomato Analyzer (TA) tool using the longitudinally and latitudinally cut fruit images. The GS performance was assessed in cross-validations of classification-based and regression-based machine learning models for CD and TA traits, respectively. The results showed the usage of TA traits and tag SNPs provide a powerful combination to predict morphology and color-related traits of Solanaceous fruits. The highest predictability of 0.89 was achieved for fruit width in pepper, with an average predictability of 0.69 over all traits. The multi-trait GS models are of slightly better predictability than single-trait models for some colorimetric traits in pepper. While model validation performs poorly on wild tomato accessions, the usage as many as one accession per wild species in the training set can increase the transferability of models to unseen populations for some traits (e.g. fruit shape for which predictability in unseen scenario increased from zero to 0.6). Overall, GS approaches can assist the selection of high-performance Solanaceous fruits in crop breeding.},
  language  = {en}
}
@article{ReinhardGeisslerBlaum2022,
  author    = {Reinhard, Johanna E. and Geißler, Katja and Blaum, Niels},
  title     = {Grass and ground dwelling beetle community responses to holistic and wildlife grazing management using a cross-fence comparison in Western Kalahari rangeland, Namibia},
  series = {Journal of insect conservation : an international journal devoted to the conservation of insects and related invertebrates},
  volume    = {26},
  journal   = {Journal of insect conservation : an international journal devoted to the conservation of insects and related invertebrates},
  publisher = {Springer},
  address   = {Dordrecht},
  issn      = {1366-638X},
  doi       = {10.1007/s10841-022-00410-6},
  pages     = {711 -- 720},
  year      = {2022},
  abstract  = {Savannahs are often branded by livestock grazing with resulting land degradation. Holistic management of livestock was proposed to contribute to biodiversity conservation by simulating native wildlife grazing behaviour. This study attempts the comparison of the impact of a holistic management regime to a wildlife grazing management regime on grass and ground-dwelling beetle species diversity on neighboring farms in Namibian rangeland. Results show that the response of biodiversity in species richness and composition to holistic management of livestock differs substantially from wildlife grazing with a positive impact. From a total of 39 identified species of ground-dwelling beetles (Coleoptera: Tenebrionidae, Carabidae) from 29 genera, eight species were found to be indicators for holistic management of livestock and three were found to be indicators for wildlife grazed rangeland. Observations suggest that holistic management of livestock may contribute to biodiversity conservation, but the differential effect of grazing management on species assemblages suggests that livestock grazing cannot replace native wildlife herbivory. Implications for insect conservation An adaptive management strategy such as holistic management used in this study shows the potential to support high beetle biodiversity. Holistic management of livestock thus aspects in favour for a sustainable form of grazing management for insect conservation even though it does not functionally replace grazing by native wildlife.},
  language  = {en}
}
@article{LiPostlBoehmeretal.2022,
  author    = {Li, Chenzhi and Postl, Alexander K. and B{\"o}hmer, Thomas and Cao, Xianyong and Dolman, Andrew M. and Herzschuh, Ulrike},
  title     = {Harmonized chronologies of a global late Quaternary pollen dataset (LegacyAge 1.0)},
  series = {Earth system science data : ESSD},
  volume    = {14},
  journal   = {Earth system science data : ESSD},
  number    = {3},
  publisher = {Copernics Publications},
  address   = {Katlenburg-Lindau},
  issn      = {1866-3508},
  doi       = {10.5194/essd-14-1331-2022},
  pages     = {1331 -- 1343},
  year      = {2022},
  abstract  = {We present a chronology framework named LegacyAge 1.0 containing harmonized chronologies for 2831 pollen records (downloaded from the Neotoma Paleoecology Database and the supplementary Asian datasets) together with their age control points and metadata in machine-readable data formats. All chronologies use the Bayesian framework implemented in Bacon version 2.5.3. Optimal parameter settings of priors (accumulation.shape, memory.strength, memory.mean, accumulation.rate, and thickness) were identified based on information in the original publication or iteratively after preliminary model inspection. The most common control points for the chronologies are radiocarbon dates (86.1 \%), calibrated by the latest calibration curves (IntCal20 and SHCal20 for the terrestrial radiocarbon dates in the Northern Hemisphere and Southern Hemisphere and Marine20 for marine materials). The original publications were consulted when dealing with outliers and inconsistencies. Several major challenges when setting up the chronologies included the waterline issue (18.8\% of records), reservoir effect (4.9 \%), and sediment deposition discontinuity (4.4 \%). Finally, we numerically compare the LegacyAge 1.0 chronologies to those published in the original publications and show that the reliability of the chronologies of 95.4\% of records could be improved according to our assessment. Our chronology framework and revised chronologies provide the opportunity to make use of the ages and age uncertainties in synthesis studies of, for example, pollen-based vegetation and climate change. The LegacyAge 1.0 dataset, including metadata, datings, harmonized chronologies, and R code used, is openaccess and available at PANGAEA (https://doi.org/10.1594/PANGAEA.933132; Li et al., 2021) and Zenodo (https://doi.org/10.5281/zenodo.5815192; Li et al., 2022), respectively.},
  language  = {en}
}
@article{GluecklerGengGrimmetal.2022,
  author    = {Gl{\"u}ckler, Ramesh and Geng, Rongwei and Grimm, Lennart and Baisheva, Izabella and Herzschuh, Ulrike and Stoof-Leichsenring, Kathleen R. and Kruse, Stefan and Andreev, Andrej Aleksandrovic and Pestryakova, Luidmila and Dietze, Elisabeth},
  title     = {Holocene wildfire and vegetation dynamics in Central Yakutia, Siberia, reconstructed from lake-sediment proxies},
  series = {Frontiers in Ecology and Evolution},
  volume    = {10},
  journal   = {Frontiers in Ecology and Evolution},
  publisher = {Frontiers Media},
  address   = {Lausanne},
  issn      = {2296-701X},
  doi       = {10.3389/fevo.2022.962906},
  pages     = {19},
  year      = {2022},
  abstract  = {Wildfires play an essential role in the ecology of boreal forests. In eastern Siberia, fire activity has been increasing in recent years, challenging the livelihoods of local communities. Intensifying fire regimes also increase disturbance pressure on the boreal forests, which currently protect the permafrost beneath from accelerated degradation. However, long-term relationships between changes in fire regime and forest structure remain largely unknown. We assess past fire-vegetation feedbacks using sedimentary proxy records from Lake Satagay, Central Yakutia, Siberia, covering the past c. 10,800 years. Results from macroscopic and microscopic charcoal analyses indicate high amounts of burnt biomass during the Early Holocene, and that the present-day, low-severity surface fire regime has been in place since c. 4,500 years before present. A pollen-based quantitative reconstruction of vegetation cover and a terrestrial plant record based on sedimentary ancient DNA metabarcoding suggest a pronounced shift in forest structure toward the Late Holocene. Whereas the Early Holocene was characterized by postglacial open larch-birch woodlands, forest structure changed toward the modern, mixed larch-dominated closed-canopy forest during the Mid-Holocene. We propose a potential relationship between open woodlands and high amounts of burnt biomass, as well as a mediating effect of dense larch forest on the climate-driven intensification of fire regimes. Considering the anticipated increase in forest disturbances (droughts, insect invasions, and wildfires), higher tree mortality may force the modern state of the forest to shift toward an open woodland state comparable to the Early Holocene. Such a shift in forest structure may result in a positive feedback on currently intensifying wildfires. These new long-term data improve our understanding of millennial-scale fire regime changes and their relationships to changes of vegetation in Central Yakutia, where the local population is already being confronted with intensifying wildfire seasons.},
  language  = {en}
}
@article{ZappaSchlafferBroccaetal.2022,
  author    = {Zappa, Luca and Schlaffer, Stefan and Brocca, Luca and Vreugdenhil, Mariette and Nendel, Claas and Dorigo, Wouter},
  title     = {How accurately can we retrieve irrigation timing and water amounts from (satellite) soil moisture?},
  series = {International journal of applied earth observation and geoinformation},
  volume    = {113},
  journal   = {International journal of applied earth observation and geoinformation},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1569-8432},
  doi       = {10.1016/j.jag.2022.102979},
  pages     = {12},
  year      = {2022},
  abstract  = {While ensuring food security worldwide, irrigation is altering the water cycle and generating numerous environmental side effects. As detailed knowledge about the timing and the amounts of water used for irrigation over large areas is still lacking, remotely sensed soil moisture has proved potential to fill this gap. However, the spatial resolution and revisit time of current satellite products represent a major limitation to accurately estimating irrigation. This work aims to systematically quantify their impact on the retrieved irrigation information, hence assessing the value of satellite soil moisture for estimating irrigation timing and water amounts. In a real-world experiment, we modeled soil moisture using actual irrigation and meteorological data, obtained from farmers and weather stations, respectively. Modeled soil moisture was compared against various remotely sensed products differing in terms of spatio-temporal resolution to test the hypothesis that high-resolution observations can disclose the irrigation signal from individual fields while coarse-scale satellite products cannot. Then, in a synthetic experiment, we systematically investigated the effect of soil moisture spatial and temporal resolution on the accuracy of irrigation estimates. The analysis was further elaborated by considering different irrigation scenarios and by adding realistic amounts of random errors in the soil moisture time series. We show that coarse-scale remotely sensed soil moisture products achieve higher correlations with rainfed simulations, while high-resolution satellite observations agree significantly better with irrigated simulations, suggesting that high-resolution satellite soil moisture can inform on field-scale (similar to 40 ha) irrigation. A thorough analysis of the synthetic dataset showed that satisfactory results, both in terms of detection (F-score > 0.8) and quantification (Pearson's correlation > 0.8), are found for noise-free soil moisture observations either with a temporal sampling up to 3 days or if at least one-third of the pixel covers the irrigated field(s). However, irrigation water amounts are systematically underestimated for temporal samplings of more than one day, and decrease proportionally to the spatial resolution, i.e., coarsening the pixel size leads to larger irrigation underestimations. Although lower spatial and temporal resolutions decrease the detection and quantification accuracies (e.g., R between 0.6 and 1 depending on the irrigation rate and spatio-temporal resolution), random errors in the soil moisture time series have a stronger negative impact (Pearson R always smaller than 0.85). As expected, better performances are found for higher irrigation rates, i.e. when more water is supplied during an irrigation event. Despite the potentially large underestimations, our results suggest that high-resolution satellite soil moisture has the potential to track and quantify irrigation, especially over regions where large volumes of irrigation water are applied to the fields, and given that low errors affect the soil moisture observations.},
  language  = {en}
}
@article{WangHeWangetal.2022,
  author    = {Wang, Enli and He, Di and Wang, Jing and Lilley, Julianne M. and Christy, Brendan and Hoffmann, Munir P. and O'Leary, Garry and Hatfield, Jerry L. and Ledda, Luigi and Deligios, Paola A. and Grant, Brian and Jing, Qi and Nendel, Claas and Kage, Henning and Qian, Budong and Rezaei, Ehsan Eyshi and Smith, Ward and Weymann, Wiebke and Ewert, Frank},
  title     = {How reliable are current crop models for simulating growth and seed yield of canola across global sites and under future climate change?},
  series = {Climatic change},
  volume    = {172},
  journal   = {Climatic change},
  number    = {1-2},
  publisher = {Springer Nature},
  address   = {Dordrecht},
  issn      = {0165-0009},
  doi       = {10.1007/s10584-022-03375-2},
  pages     = {22},
  year      = {2022},
  abstract  = {To better understand how climate change might influence global canola production, scientists from six countries have completed the first inter-comparison of eight crop models for simulating growth and seed yield of canola, based on experimental data from six sites across five countries. A sensitivity analysis was conducted with a combination of five levels of atmospheric CO2 concentrations, seven temperature changes, five precipitation changes, together with five nitrogen application rates. Our results were in several aspects different from those of previous model inter-comparison studies for wheat, maize, rice, and potato crops. A partial model calibration only on phenology led to very poor simulation of aboveground biomass and seed yield of canola, even from the ensemble median or mean. A full calibration with additional data of leaf area index, biomass, and yield from one treatment at each site reduced simulation error of seed yield from 43.8 to 18.0\%, but the uncertainty in simulation results remained large. Such calibration (with data from one treatment) was not able to constrain model parameters to reduce simulation uncertainty across the wide range of environments. Using a multi-model ensemble mean or median reduced the uncertainty of yield simulations, but the simulation error remained much larger than observation errors, indicating no guarantee that the ensemble mean/median would predict the correct responses. Using multi-model ensemble median, canola yield was projected to decline with rising temperature (2.5-5.7\% per degrees C), but to increase with increasing CO2 concentration (4.6-8.3\% per 100-ppm), rainfall (2.1-6.1\% per 10\% increase), and nitrogen rates (1.3-6.0\% per 10\% increase) depending on locations. Due to the large uncertainty, these results need to be treated with caution. We further discuss the need to collect new data to improve modelling of several key physiological processes of canola for increased confidence in future climate impact assessments.},
  language  = {en}
}
@article{CaoTianHerzschuhetal.2022,
  author    = {Cao, Xianyong and Tian, Fang and Herzschuh, Ulrike and Ni, Jian and Xu, Qinghai and Li, Wenjia and Zhang, Yanrong and Luo, Mingyu and Chen, Fahu},
  title     = {Human activities have reduced plant diversity in eastern China over the last two millennia},
  series = {Global change biology},
  volume    = {28},
  journal   = {Global change biology},
  number    = {16},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {1354-1013},
  doi       = {10.1111/gcb.16274},
  pages     = {4962 -- 4976},
  year      = {2022},
  abstract  = {Understanding the history and regional singularities of human impact on vegetation is key to developing strategies for sustainable ecosystem management. In this study, fossil and modern pollen datasets from China are employed to investigate temporal changes in pollen composition, analogue quality, and pollen diversity during the Holocene. Anthropogenic disturbance and vegetation's responses are also assessed. Results reveal that pollen assemblages from non-forest communities fail to provide evidence of human impact for the western part of China (annual precipitation less than 400 mm and/or elevation more than 3000 m.a.s.l.), as inferred from the stable quality of modern analogues, principal components, and diversity of species and communities throughout the Holocene. For the eastern part of China, the proportion of fossil pollen spectra with good modern analogues increases from ca. 50\% to ca. 80\% during the last 2 millennia, indicating an enhanced intensity of anthropogenic disturbance on vegetation. This disturbance has caused the pollen spectra to become taxonomically less diverse over space (reduced abundances of arboreal taxa and increased abundances of herbaceous taxa), highlighting a reduced south-north differentiation and divergence from past vegetation between regions in the eastern part of China. We recommend that care is taken in eastern China when basing the development of ecosystem management strategies on vegetation changes in the region during the last 2000 years, since humans have significantly disturbed the vegetation during this period.},
  language  = {en}
}
@article{HermanussenGrothScheffler2022,
  author    = {Hermanussen, Michael and Groth, Detlef and Scheffler, Christiane},
  title     = {Human growth data analyses and statistics},
  series = {Human biology and public health},
  volume    = {2021},
  journal   = {Human biology and public health},
  number    = {3, Summer School Supplement},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  issn      = {2748-9957},
  doi       = {10.52905/hbph2021.3.29},
  pages     = {4},
  year      = {2022},
  abstract  = {Students learn by repetition. Repetition is essential, but repetition needs questioning, and questioning the repertoire belongs to the essential tasks of student education. Guiding students to questioning was and is our prime motive to offer our International Student Summer Schools. The data were critically discussed among the students, in the twilight of Just So Stories, common knowledge, and prompted questioning of contemporary solutions. For these schools, the students bring their own data, carry their preliminary concepts, and in group discussions, they may have to challenge these concepts. Catch-up growth is known to affect long bone growth, but different opinions exist to what extent it also affects body proportions. Skeletal age and dental development are considered appropriate measures of maturation, but it appears that both system develop independently and are regulated by different mechanisms. Body weight distributions are assumed to be skewed, yet, historic data disproved this assumption. Many discussions focused on current ideas of global growth standards as a common yardstick for all populations world-wide, with new statistical tools being developed including network reconstruction and evaluation of the reconstructs to determine the confidence of graph prediction methods.},
  language  = {en}
}
@article{MontesOsunaCernavaGomezLamaCabanasetal.2022,
  author    = {Montes-Osuna, Nuria and Cernava, Tomislav and Gomez-Lama Cabanas, Carmen and Berg, Gabriele and Mercado-Blanco, Jesus},
  title     = {Identification of volatile organic compounds emitted by two beneficial endophytic pseudomonas strains from olive roots},
  series = {Plants},
  volume    = {11},
  journal   = {Plants},
  number    = {3},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2223-7747},
  doi       = {10.3390/plants11030318},
  pages     = {14},
  year      = {2022},
  abstract  = {The production of volatile organic compounds (VOCs) represents a promising strategy of plant-beneficial bacteria to control soil-borne phytopathogens. Pseudomonas sp. PICF6 and Pseudomonas simiae PICF7 are two indigenous inhabitants of olive roots displaying effective biological control against Verticillium dahliae. Additionally, strain PICF7 is able to promote the growth of barley and Arabidopsis thaliana, VOCs being involved in the growth of the latter species. In this study, the antagonistic capacity of these endophytic bacteria against relevant phytopathogens (Verticillium spp., Rhizoctonia solani, Sclerotinia sclerotiorum and Fusarium oxysporum f.sp. lycopersici) was assessed. Under in vitro conditions, PICF6 and PICF7 were only able to antagonize representative isolates of V. dahliae and V. longisporum. Remarkably, both strains produced an impressive portfolio of up to twenty VOCs, that included compounds with reported antifungal (e.g., 1-undecene, (methyldisulfanyl) methane and 1-decene) or plant growth promoting (e.g., tridecane, 1-decene) activities. Moreover, their volatilomes differed strongly in the absence and presence of V. dahliae. For example, when co incubated with the defoliating pathotype of V. dahliae, the antifungal compound 4-methyl-2,6-bis(2-methyl-2-propanyl)phenol was produced. Results suggest that volatiles emitted by these endophytes may differ in their modes of action, and that potential benefits for the host needs further investigation in planta.},
  language  = {en}
}
@article{KamaliStellaBergMohnickeetal.2022,
  author    = {Kamali, Bahareh and Stella, Tommaso and Berg-Mohnicke, Michael and Pickert, J{\"u}rgen and Groh, Jannis and Nendel, Claas},
  title     = {Improving the simulation of permanent grasslands across Germany by using multi-objective uncertainty-based calibration of plant-water dynamics},
  series = {European journal of agronomy},
  volume    = {134},
  journal   = {European journal of agronomy},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1161-0301},
  doi       = {10.1016/j.eja.2022.126464},
  pages     = {17},
  year      = {2022},
  abstract  = {The dynamics of grassland ecosystems are highly complex due to multifaceted interactions among their soil, water, and vegetation components. Precise simulations of grassland productivity therefore rely on accurately estimating a variety of parameters that characterize different processes of these systems. This study applied three calibration schemes - a Single-Objective (SO-SUFI2), a Multi-Objective Pareto (MO-Pareto), and, a novel Uncertainty-Based Multi-Objective (MO-SUFI2) - to estimate the parameters of MONICA (Model for Nitrogen and Carbon Simulation) agro-ecosystem model in grassland ecosystems across Germany. The MO-Pareto model is based on a traditional Pareto optimality concept, while the MO-SUFI2 optimizes multiple target variables considering their level of prediction uncertainty. We used measurements of leaf area index, aboveground biomass, and soil moisture from experimental data at five sites with different intensities of cutting regimes (from two to five cutting events per season) to evaluate model performance. Both MO-Pareto and MO-SUFI2 outperformed SO-SUFI2 during calibration and validation. The comparison of the two MO approaches shows that they do not necessarily conflict with each other, but MO-SUFI2 provides complementary information for better estimations of model parameter uncertainty. We used the obtained parameter ranges to simulate grassland productivity across Germany under different cutting regimes and quantified the uncertainty associated with estimated productivity across regions. The results showed higher uncertainty in intensively managed grasslands compared to extensively managed grasslands, partially due to a lack of high-resolution input information concerning cutting dates. Furthermore, the additional information on the quantified uncertainty provided by our proposed MO-SUFI2 method adds deeper insights on confidence levels of estimated productivity. Benefiting from additional management data collected at high resolution and ground measurements on the composition of grassland species mixtures appear to be promising solutions to reduce uncertainty and increase model reliability.},
  language  = {en}
}
@article{ApriyantoCompartZimmermannetal.2022,
  author    = {Apriyanto, Ardha and Compart, Julia and Zimmermann, Vincent and Alseekh, Saleh and Fernie, Alisdair R. and Fettke, J{\"o}rg},
  title     = {Indication that starch and sucrose are biomarkers for oil yield in oil palm (Elaeis guineensis Jacq.)},
  series = {Food chemistry},
  volume    = {393},
  journal   = {Food chemistry},
  publisher = {Elsevier},
  address   = {New York, NY [u.a.]},
  issn      = {0308-8146},
  doi       = {10.1016/j.foodchem.2022.133361},
  pages     = {11},
  year      = {2022},
  abstract  = {Oil palm (Elaeis guineensis Jacq.) is the most productive oil-producing crop per hectare of land. The oil that accumulates in the mesocarp tissue of the fruit is the highest observed among fruit-producing plants. A comparative analysis between high-, medium-, and low-yielding oil palms, particularly during fruit development, revealed unique characteristics. Metabolomics analysis was able to distinguish accumulation patterns defining of the various developmental stages and oil yield. Interestingly, high- and medium-yielding oil palms exhibited substantially increased sucrose levels compared to low-yielding palms. In addition, parameters such as starch granule morphology, granule size, total starch content, and starch chain length distribution (CLD) differed significantly among the oil yield categories with a clear correlation between oil yield and various starch parameters. These results provide new insights into carbohydrate and starch metabolism for biosynthesis of oil palm fruits, indicating that starch and sucrose can be used as novel, easy-to-analyze, and reliable biomarker for oil yield.},
  language  = {en}
}
@article{OberkoflerBaeurle2022,
  author    = {Oberkofler, Vicky and B{\"a}urle, Isabel},
  title     = {Inducible epigenome editing probes for the role of histone H3K4 methylation in Arabidopsis heat stress memory},
  series = {Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants},
  volume    = {189},
  journal   = {Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants},
  number    = {2},
  publisher = {Oxford University Press},
  address   = {Oxford},
  issn      = {0032-0889},
  doi       = {10.1093/plphys/kiac113},
  pages     = {703 -- 714},
  year      = {2022},
  abstract  = {A temperature-inducible epigenome editing system to knock down histone methylation can be used to study the role of histone H3K4 methylation during heat stress memory in Arabidopsis. <br /> Histone modifications play a crucial role in the integration of environmental signals to mediate gene expression outcomes. However, genetic and pharmacological interference often causes pleiotropic effects, creating the urgent need for methods that allow locus-specific manipulation of histone modifications, preferably in an inducible manner. Here, we report an inducible system for epigenome editing in Arabidopsis (Arabidopsis thaliana) using a heat-inducible dCas9 to target a JUMONJI (JMJ) histone H3 lysine 4 (H3K4) demethylase domain to a locus of interest. As a model locus, we target the ASCORBATE PEROXIDASE2 (APX2) gene that shows transcriptional memory after heat stress (HS), correlating with H3K4 hyper-methylation. We show that dCas9-JMJ is targeted in a HS-dependent manner to APX2 and that the HS-induced overaccumulation of H3K4 trimethylation (H3K4me3) decreases when dCas9-JMJ binds to the locus. This results in reduced HS-mediated transcriptional memory at the APX2 locus. Targeting an enzymatically inactive JMJ protein in an analogous manner affected transcriptional memory less than the active JMJ protein; however, we still observed a decrease in H3K4 methylation levels. Thus, the inducible targeting of dCas9-JMJ to APX2 was effective in reducing H3K4 methylation levels. As the effect was not fully dependent on enzyme activity of the eraser domain, the dCas9-JMJ fusion protein may act in part independently of its demethylase activity. This underlines the need for caution in the design and interpretation of epigenome editing studies. We expect our versatile inducible epigenome editing system to be especially useful for studying temporal dynamics of chromatin modifications.},
  language  = {en}
}
@article{LaunDuffusWahlefeldetal.2022,
  author    = {Laun, Konstantin and Duffus, Benjamin R. and Wahlefeld, Stefan and Katz, Sagie and Belger, Dennis Heinz and Hildebrandt, Peter and Mroginski, Maria Andrea and Leimk{\"u}hler, Silke and Zebger, Ingo},
  title     = {Infrared spectroscopy flucidates the inhibitor binding sites in a metal-dependent formate dehydrogenase},
  series = {Chemistry - a European journal},
  journal   = {Chemistry - a European journal},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0947-6539},
  doi       = {10.1002/chem.202201091},
  pages     = {8},
  year      = {2022},
  abstract  = {Biological carbon dioxide (CO2) reduction is an important step by which organisms form valuable energy-richer molecules required for further metabolic processes. The Mo-dependent formate dehydrogenase (FDH) from Rhodobacter capsulatus catalyzes reversible formate oxidation to CO2 at a bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor. To elucidate potential substrate binding sites relevant for the mechanism, we studied herein the interaction with the inhibitory molecules azide and cyanate, which are isoelectronic to CO2 and charged as formate. We employed infrared (IR) spectroscopy in combination with density functional theory (DFT) and inhibition kinetics. One distinct inhibitory molecule was found to bind to either a non-competitive or a competitive binding site in the secondary coordination sphere of the active site. Site-directed mutagenesis of key amino acid residues in the vicinity of the bis-MGD cofactor revealed changes in both non-competitive and competitive binding, whereby the inhibitor is in case of the latter interaction presumably bound between the cofactor and the adjacent Arg587.},
  language  = {en}
}
@article{DreymannWuenscheSabrowskietal.2022,
  author    = {Dreymann, Nico and Wuensche, Julia and Sabrowski, Wiebke and Moeller, Anja and Czepluch, Denise and Vu Van, Dana and F{\"u}ssel, Susanne and Menger, Marcus M.},
  title     = {Inhibition of Human Urokinase-Type Plasminogen Activator (uPA) Enzyme Activity and Receptor Binding by DNA Aptamers as Potential Therapeutics through Binding to the Different Forms of uPA},
  series = {International journal of molecular sciences},
  volume    = {23},
  journal   = {International journal of molecular sciences},
  number    = {9},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {1661-6596},
  doi       = {10.3390/ijms23094890},
  pages     = {22},
  year      = {2022},
  abstract  = {Urokinase-type plasminogen activator is widely discussed as a marker for cancer prognosis and diagnosis and as a target for cancer therapies. Together with its receptor, uPA plays an important role in tumorigenesis, tumor progression and metastasis. In the present study, systematic evolution of ligands by exponential enrichment (SELEX) was used to select single-stranded DNA aptamers targeting different forms of human uPA. Selected aptamers allowed the distinction between HMW-uPA and LMW-uPA, and therefore, presumably, have different binding regions. Here, uPAapt-02-FR showed highly affine binding with a K-D of 0.7 nM for HMW-uPA and 21 nM for LMW-uPA and was also able to bind to pro-uPA with a K-D of 14 nM. Furthermore, no cross-reactivity to mouse uPA or tissue-type plasminogen activator (tPA) was measured, demonstrating high specificity. Suppression of the catalytic activity of uPA and inhibition of uPAR-binding could be demonstrated through binding with different aptamers and several of their truncated variants. Since RNA aptamers are already known to inhibit uPA-uPAR binding and other pathological functions of the uPA system, these aptamers represent a novel, promising tool not only for detection of uPA but also for interfering with the pathological functions of the uPA system by additionally inhibiting uPA activity.},
  language  = {en}
}
@article{StelbrinkvonRintelenRichteretal.2022,
  author    = {Stelbrink, Bj{\"o}rn and von Rintelen, Thomas and Richter, Kirsten and Finstermeier, Knut and Frahnert, Sylke and Cracraft, Joel and Hofreiter, Michael},
  title     = {Insights into the geographical origin and phylogeographical patterns of Paradisaea birds-of-paradise},
  series = {Zoological journal of the Linnean Society},
  volume    = {196},
  journal   = {Zoological journal of the Linnean Society},
  number    = {4},
  publisher = {Oxford Univ. Press},
  address   = {Oxford},
  issn      = {0024-4082},
  doi       = {10.1093/zoolinnean/zlac010},
  pages     = {1394 -- 1407},
  year      = {2022},
  abstract  = {Birds-of-paradise represent a textbook example for geographical speciation and sexual selection. Perhaps the most iconic genus is Paradisaea, which is restricted to New Guinea and a few surrounding islands. Although several species concepts have been applied in the past to disentangle the different entities within this genus, no attempt has been made so far to uncover phylogeographical patterns based on a genetic dataset that includes multiple individuals per species. Here, we applied amplicon sequencing for the mitochondrial fragment Cytb for a total of 69 museum specimens representing all seven Paradisaea species described and inferred both phylogenetic relationships and colonization pathways across the island. Our analyses show that the most recent common ancestor of the diverging lineages within Paradisaea probably originated in the Late Miocene in the eastern part of the Central Range and suggest that tectonic processes played a key role in shaping the diversification and distribution of species. All species were recovered as monophyletic, except for those within the apoda-minor-raggiana clade, which comprises the allopatric and parapatric species P. apoda, P. minor and P. raggiana. The comparatively young divergence times, together with possible instances of mitochondrial introgression and incomplete lineage sorting, suggest recent speciation in this clade.},
  language  = {en}
}
@article{OlimiKusstatscherWicaksonoetal.2022,
  author    = {Olimi, Expedito and Kusstatscher, Peter and Wicaksono, Wisnu Adi and Abdelfattah, Ahmed and Cernava, Tomislav and Berg, Gabriele},
  title     = {Insights into the microbiome assembly during different growth stages and storage of strawberry plants},
  series = {Environmental microbiome},
  volume    = {17},
  journal   = {Environmental microbiome},
  number    = {1},
  publisher = {BMC},
  address   = {London},
  issn      = {2524-6372},
  doi       = {10.1186/s40793-022-00415-3},
  pages     = {15},
  year      = {2022},
  abstract  = {Background: Microbiome assembly was identified as an important factor for plant growth and health, but this process is largely unknown, especially for the fruit microbiome. Therefore, we analyzed strawberry plants of two cultivars by focusing on microbiome tracking during the different growth stages and storage using amplicon sequencing, qPCR, and microscopic approaches. <br /> Results: Strawberry plants carried a highly diverse microbiome, therein the bacterial families Sphingomonadaceae (25\%), Pseudomonadaceae (17\%), and Burkholderiaceae (11\%); and the fungal family Mycosphaerella (45\%) were most abundant. All compartments were colonized by high number of bacteria and fungi (10(7)-10(10) marker gene copies per g fresh weight), and were characterized by high microbial diversity (6049 and 1501 ASVs); both were higher for the belowground samples than in the phyllosphere. Compartment type was the main driver of microbial diversity, structure, and abundance (bacterial: 45\%; fungal: 61\%) when compared to the cultivar (1.6\%; 2.2\%). Microbiome assembly was strongly divided for belowground habitats and the phyllosphere; only a low proportion of the microbiome was transferred from soil via the rhizosphere to the phyllosphere. During fruit development, we observed the highest rates of microbial transfer from leaves and flowers to ripe fruits, where most of the bacteria occured inside the pulp. In postharvest fruits, microbial diversity decreased while the overall abundance increased. Developing postharvest decay caused by Botrytis cinerea decreased the diversity as well, and induced a reduction of potentially beneficial taxa. <br /> Conclusion: Our findings provide insights into microbiome assembly in strawberry plants and highlight the importance of microbe transfer during fruit development and storage with potential implications for food health and safety.},
  language  = {en}
}
@article{TebbeOttensmannHavensteinetal.2022,
  author    = {Tebbe, Jonas and Ottensmann, Meinolf and Havenstein, Katja and Efstratiou, Artemis and Lenz, Tobias L. and Caspers, Barbara A. and Forcada, Jaume and Tiedemann, Ralph and Hoffman, Joseph},
  title     = {Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals},
  series = {Scientific reports},
  volume    = {12},
  journal   = {Scientific reports},
  number    = {1},
  publisher = {Nature Publishing Group},
  address   = {London},
  issn      = {2045-2322},
  doi       = {10.1038/s41598-022-21658-7},
  pages     = {14},
  year      = {2022},
  abstract  = {The major histocompatibility complex (MHC) is a group of genes comprising one of the most important components of the vertebrate immune system. Consequently, there has been much interest in characterising MHC variation and its relationship with fitness in a variety of species. Due to the exceptional polymorphism of MHC genes, careful PCR primer design is crucial for capturing all of the allelic variation present in a given species. We therefore developed intronic primers to amplify the full-length 267 bp protein-coding sequence of the MHC class II DQB exon 2 in the Antarctic fur seal. We then characterised patterns of MHC variation among mother-offspring pairs from two breeding colonies and detected 19 alleles among 771 clone sequences from 56 individuals. The distribution of alleles within and among individuals was consistent with a single-copy, classical DQB locus showing Mendelian inheritance. Amino acid similarity at the MHC was significantly associated with genome-wide relatedness, but no relationship was found between MHC heterozygosity and genome-wide heterozygosity. Finally, allelic diversity was several times higher than reported by a previous study based on partial exon sequences. This difference appears to be related to allele-specific amplification bias, implying that primer design can strongly impact the inference of MHC diversity.},
  language  = {en}
}
@article{McHuronAdamczakArnouldetal.2022,
  author    = {McHuron, Elizabeth A. and Adamczak, Stephanie and Arnould, John P. Y. and Ashe, Erin and Booth, Cormac and Bowen, W. Don and Christiansen, Fredrik and Chudzinska, Magda and Costa, Daniel P. and Fahlman, Andreas and Farmer, Nicholas A. and Fortune, Sarah M. E. and Gallagher, Cara A. and Keen, Kelly A. and Madsen, Peter T. and McMahon, Clive R. and Nabe-Nielsen, Jacob and Noren, Dawn P. and Noren, Shawn R. and Pirotta, Enrico and Rosen, David A. S. and Speakman, Cassie N. and Villegas-Amtmann, Stella and Williams, Rob},
  title     = {Key questions in marine mammal bioenergetics},
  series = {Conservation physiology},
  volume    = {10},
  journal   = {Conservation physiology},
  number    = {1},
  publisher = {Oxford Univ. Press},
  address   = {Oxford},
  issn      = {2051-1434},
  doi       = {10.1093/conphys/coac055},
  pages     = {17},
  year      = {2022},
  abstract  = {Bioenergetic approaches are increasingly used to understand how marine mammal populations could be affected by a changing and disturbed aquatic environment. There remain considerable gaps in our knowledge of marine mammal bioenergetics, which hinder the application of bioenergetic studies to inform policy decisions. We conducted a priority-setting exercise to identify high-priority unanswered questions in marine mammal bioenergetics, with an emphasis on questions relevant to conservation and management. Electronic communication and a virtual workshop were used to solicit and collate potential research questions from the marine mammal bioenergetic community. From a final list of 39 questions, 11 were identified as 'key'questions because they received votes from at least 50\% of survey participants. Key questions included those related to energy intake (prey landscapes, exposure to human activities) and expenditure (field metabolic rate, exposure to human activities, lactation, time-activity budgets), energy allocation priorities, metrics of body condition and relationships with survival and reproductive success and extrapolation of data from one species to another. Existing tools to address key questions include labelled water, animal-borne sensors, mark-resight data from long-term research programs, environmental DNA and unmanned vehicles. Further validation of existing approaches and development of new methodologies are needed to comprehensively address some key questions, particularly for cetaceans. The identification of these key questions can provide a guiding framework to set research priorities, which ultimately may yield more accurate information to inform policies and better conserve marine mammal populations.},
  language  = {en}
}
@article{OstermannMiyashitaKoenigPernatetal.2022,
  author    = {Ostermann-Miyashita, Emu-Felicitas and K{\"o}nig, Hannes J. and Pernat, Nadja and Bellingrath-Kimura, Sonoko Dorothea and Hibler, Sophia and Kiffner, Christian},
  title     = {Knowledge of returning wildlife species and willingness to participate in citizen science projects among wildlife park visitors in Germany},
  series = {People and nature},
  volume    = {4},
  journal   = {People and nature},
  number    = {5},
  publisher = {British Ecological Society; Wiley},
  address   = {London; Hoboken, NJ},
  issn      = {2575-8314},
  doi       = {10.1002/pan3.10379},
  pages     = {1201 -- 1215},
  year      = {2022},
  abstract  = {Successful conservation efforts have led to recent increases of large mammals such as European bison Bison bonasus, moose Alces alces and grey wolf Canis lupus and their return to former habitats in central Europe. While embraced by some, the recovery of these species is a controversial topic and holds potential for human-wildlife conflicts. Involving the public has been suggested to be an effective method for monitoring wildlife and mitigating associated conflicts. To assess two interrelated prerequisites for engaging people in Citizen Science (CS)-knowledge of returning species and respondents' readiness to participate in CS activities for monitoring and managing these species-we conducted a survey (questionnaire) in two wildlife parks located in different states of Germany. Based on 472 complete questionnaires, we developed generalized linear models to understand how sociodemographic variables and exposure to the species affected visitors' knowledge of each species, and to investigate if sociodemographic variables and knowledge influenced the likelihood of visitors to participate in CS activities. Almost all visitors were aware of the returning wolf population, while knowledge and awareness about bison and moose were significantly lower. Knowledge of the two herbivores differed geographically (higher knowledge of moose in the north-eastern state), possibly indicating a positive association between exposure to the species and knowledge. However, models generally performed poorly in predicting knowledge about wildlife, suggesting that such specific knowledge is insufficiently explained by sociodemographic variables. Our model, which explained stated willingness in CS indicated that younger participants and those with higher knowledge scores in the survey were more willing to engage in CS activities. Overall, our analyses highlight how exposure to large mammals, knowledge about wildlife and human demographics are interrelated-insights that are helpful for effectively recruiting citizen scientists for wildlife conservation. Read the free Plain Language Summary for this article on the Journal blog.},
  language  = {en}
}
@article{BizicIonescuKarnataketal.2022,
  author    = {Bizic, Mina and Ionescu, Danny and Karnatak, Rajat and Musseau, Camille L. and Onandia, Gabriela and Berger, Stella A. and Nejstgaard, Jens C. and Lischeid, Gunnar and Gessner, Mark O. and Wollrab, Sabine and Grossart, Hans-Peter},
  title     = {Land-use type temporarily affects active pond community structure but not gene expression patterns},
  series = {Molecular ecology},
  volume    = {31},
  journal   = {Molecular ecology},
  number    = {6},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0962-1083},
  doi       = {10.1111/mec.16348},
  pages     = {1716 -- 1734},
  year      = {2022},
  abstract  = {Changes in land use and agricultural intensification threaten biodiversity and ecosystem functioning of small water bodies. We studied 67 kettle holes (KH) in an agricultural landscape in northeastern Germany using landscape-scale metatranscriptomics to understand the responses of active bacterial, archaeal and eukaryotic communities to land-use type. These KH are proxies of the millions of small standing water bodies of glacial origin spread across the northern hemisphere. Like other landscapes in Europe, the study area has been used for intensive agriculture since the 1950s. In contrast to a parallel environmental DNA study that suggests the homogenization of biodiversity across KH, conceivably resulting from long-lasting intensive agriculture, land-use type affected the structure of the active KH communities during spring crop fertilization, but not a month later. This effect was more pronounced for eukaryotes than for bacteria. In contrast, gene expression patterns did not differ between months or across land-use types, suggesting a high degree of functional redundancy across the KH communities. Variability in gene expression was best explained by active bacterial and eukaryotic community structures, suggesting that these changes in functioning are primarily driven by interactions between organisms. Our results indicate that influences of the surrounding landscape result in temporary changes in the activity of different community members. Thus, even in KH where biodiversity has been homogenized, communities continue to respond to land management. This potential needs to be considered when developing sustainable management options for restoration purposes and for successful mitigation of further biodiversity loss in agricultural landscapes.},
  language  = {en}
}
@article{LeinsGrimmDrechsler2022,
  author    = {Leins, Johannes A. and Grimm, Volker and Drechsler, Martin},
  title     = {Large-scale PVA modeling of insects in cultivated grasslands},
  series = {Ecology and evolution},
  volume    = {12},
  journal   = {Ecology and evolution},
  number    = {7},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {2045-7758},
  doi       = {10.1002/ece3.9063},
  pages     = {17},
  year      = {2022},
  abstract  = {In many species, dispersal is decisive for survival in a changing climate. Simulation models for population dynamics under climate change thus need to account for this factor. Moreover, large numbers of species inhabiting agricultural landscapes are subject to disturbances induced by human land use. We included dispersal in the HiLEG model that we previously developed to study the interaction between climate change and agricultural land use in single populations. Here, the model was parameterized for the large marsh grasshopper (LMG) in cultivated grasslands of North Germany to analyze (1) the species development and dispersal success depending on the severity of climate change in subregions, (2) the additional effect of grassland cover on dispersal success, and (3) the role of dispersal in compensating for detrimental grassland mowing. Our model simulated population dynamics in 60-year periods (2020-2079) on a fine temporal (daily) and high spatial (250 x 250 m(2)) scale in 107 subregions, altogether encompassing a range of different grassland cover, climate change projections, and mowing schedules. We show that climate change alone would allow the LMG to thrive and expand, while grassland cover played a minor role. Some mowing schedules that were harmful to the LMG nevertheless allowed the species to moderately expand its range. Especially under minor climate change, in many subregions dispersal allowed for mowing early in the year, which is economically beneficial for farmers. More severe climate change could facilitate LMG expansion to uninhabited regions but would require suitable mowing schedules along the path. These insights can be transferred to other species, given that the LMG is considered a representative of grassland communities. For more specific predictions on the dynamics of other species affected by climate change and land use, the publicly available HiLEG model can be easily adapted to the characteristics of their life cycle.},
  language  = {en}
}
@article{SchulteMeucciStoofLeichsenringetal.2022,
  author    = {Schulte, Luise and Meucci, Stefano and Stoof-Leichsenring, Kathleen R. and Heitkam, Tony and Schmidt, Nicola and von Hippel, Barbara and Andreev, Andrei A. and Diekmann, Bernhard and Biskaborn, Boris and Wagner, Bernd and Melles, Martin and Pestryakova, Lyudmila A. and Alsos, Inger G. and Clarke, Charlotte and Krutovsky, Konstantin and Herzschuh, Ulrike},
  title     = {Larix species range dynamics in Siberia since the Last Glacial captured from sedimentary ancient DNA},
  series = {Communications biology},
  volume    = {5},
  journal   = {Communications biology},
  number    = {1},
  publisher = {Springer Nature},
  address   = {London},
  issn      = {2399-3642},
  doi       = {10.1038/s42003-022-03455-0},
  pages     = {11},
  year      = {2022},
  abstract  = {Climate change is expected to cause major shifts in boreal forests which are in vast areas of Siberia dominated by two species of the deciduous needle tree larch (Larix). The species differ markedly in their ecosystem functions, thus shifts in their respective ranges are of global relevance. However, drivers of species distribution are not well understood, in part because paleoecological data at species level are lacking. This study tracks Larix species distribution in time and space using target enrichment on sedimentary ancient DNA extracts from eight lakes across Siberia. We discovered that Larix sibirica, presently dominating in western Siberia, likely migrated to its northern distribution area only in the Holocene at around 10,000 years before present (ka BP), and had a much wider eastern distribution around 33 ka BP. Samples dated to the Last Glacial Maximum (around 21 ka BP), consistently show genotypes of L. gmelinii. Our results suggest climate as a strong determinant of species distribution in Larix and provide temporal and spatial data for species projection in a changing climate. Using ancient sedimentary DNA from up to 50 kya, dramatic distributional shifts are documented in two dominant boreal larch species, likely guided by environmental changes suggesting climate as a strong determinant of species distribution.},
  language  = {en}
}
@article{AndreevNazarovaLenzetal.2022,
  author    = {Andreev, Andrei and Nazarova, Larisa B. and Lenz, Marlene M. and B{\"o}hmer, Thomas and Syrykh, Ludmila and Wagner, Bernd and Melles, Martin and Pestryakova, Luidmila A. and Herzschuh, Ulrike},
  title     = {Late Quaternary paleoenvironmental reconstructions from sediments of Lake Emanda (Verkhoyansk Mountains, East Siberia)},
  series = {Journal of quaternary science : JQS},
  volume    = {37},
  journal   = {Journal of quaternary science : JQS},
  number    = {5},
  publisher = {Wiley},
  address   = {New York, NY [u.a.]},
  issn      = {0267-8179},
  doi       = {10.1002/jqs.3419},
  pages     = {884 -- 899},
  year      = {2022},
  abstract  = {Continuous pollen and chironomid records from Lake Emanda (65 degrees 17'N, 135 degrees 45'E) provide new insights into the Late Quaternary environmental history of the Yana Highlands (Yakutia). Larch forest with shrubs (alders, pines, birches) dominated during the deposition of the lowermost sediments suggesting its Early Weichselian [Marine Isotope Stage (MIS) 5] age. Pollen- and chironomid-based climate reconstructions suggest July temperatures (T-July) slightly lower than modern. Gradually increasing amounts of herb pollen and cold stenotherm chironomid head capsules reflect cooler and drier environments, probably during the termination of MIS 5. T-July dropped to 8 degrees C. Mostly treeless vegetation is reconstructed during MIS 3. Tundra and steppe communities dominated during MIS 2. Shrubs became common after similar to 14.5 ka BP but herb-dominated habitats remained until the onset of the Holocene. Larch forests with shrub alder and dwarf birch dominated after the Holocene onset, ca. 11.7 ka BP. Decreasing amounts of shrub pollen during the Lateglacial are assigned to the Older Dryas and Younger Dryas with T-July similar to 7.5 degrees C. T-July increased up to 13 degrees C. Shrub stone pine was present after similar to 7.5 ka BP. The vegetation has been similar to modern since ca. 5.8 ka BP. Chironomid diversity and concentration in the sediments increased towards the present day, indicating the development of richer hydrobiological communities in response to the Holocene thermal maximum.},
  language  = {en}
}
@article{AgarwalWarmtHenkeletal.2022,
  author    = {Agarwal, Saloni and Warmt, Christian and Henkel, J{\"o}rg and Schrick, Livia and Nitsche, Andreas and Bier, Frank Fabian},
  title     = {Lateral flow-based nucleic acid detection of SARS-CoV-2 using enzymatic incorporation of biotin-labeled dUTP for POCT use},
  series = {Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry, Analusis and Quimica analitica},
  volume    = {414},
  journal   = {Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry, Analusis and Quimica analitica},
  number    = {10},
  publisher = {Springer},
  address   = {Heidelberg},
  issn      = {1618-2642},
  doi       = {10.1007/s00216-022-03880-4},
  pages     = {3177 -- 3186},
  year      = {2022},
  abstract  = {The degree of detrimental effects inflicted on mankind by the COVID-19 pandemic increased the need to develop ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, and Deliverable) POCT (point of care testing) to overcome the current and any future pandemics. Much effort in research and development is currently advancing the progress to overcome the diagnostic pressure built up by emerging new pathogens. LAMP (loop-mediated isothermal amplification) is a well-researched isothermal technique for specific nucleic acid amplification which can be combined with a highly sensitive immunochromatographic readout via lateral flow assays (LFA). Here we discuss LAMP-LFA robustness, sensitivity, and specificity for SARS-CoV-2 N-gene detection in cDNA and clinical swab-extracted RNA samples. The LFA readout is designed to produce highly specific results by incorporation of biotin and FITC labels to 11-dUTP and LF (loop forming forward) primer, respectively. The LAMP-LFA assay was established using cDNA for N-gene with an accuracy of 95.65\%. To validate the study, 82 SARS-CoV-2-positive RNA samples were tested. Reverse transcriptase (RT)-LAMP-LFA was positive for the RNA samples with an accuracy of 81.66\%; SARS-CoV-2 viral RNA was detected by RT-LAMP-LFA for as low as CT-33. Our method reduced the detection time to 15 min and indicates therefore that RT-LAMP in combination with LFA represents a promising nucleic acid biosensing POCT platform that combines with smartphone based semi-quantitative data analysis.},
  language  = {en}
}
@article{HerzschuhLiBoehmeretal.2022,
  author    = {Herzschuh, Ulrike and Li, Chenzhi and Boehmer, Thomas and Postl, Alexander K. and Heim, Birgit and Andreev, Andrei A. and Cao, Xianyong and Wieczorek, Mareike and Ni, Jian},
  title     = {LegacyPollen 1.0},
  series = {Earth system science data : ESSD},
  volume    = {14},
  journal   = {Earth system science data : ESSD},
  number    = {7},
  publisher = {Copernicus},
  address   = {G{\"o}ttingen},
  issn      = {1866-3508},
  doi       = {10.5194/essd-14-3213-2022},
  pages     = {3213 -- 3227},
  year      = {2022},
  abstract  = {Here we describe the LegacyPollen 1.0, a dataset of 2831 fossil pollen records with metadata, a harmonized taxonomy, and standardized chronologies. A total of 1032 records originate from North America, 1075 from Europe, 488 from Asia, 150 from Latin America, 54 from Africa, and 32 from the Indo-Pacific. The pollen data cover the late Quaternary (mostly the Holocene). The original 10 110 pollen taxa names (including variations in the notations) were harmonized to 1002 terrestrial taxa (including Cyperaceae), with woody taxa and major herbaceous taxa harmonized to genus level and other herbaceous taxa to family level. The dataset is valuable for synthesis studies of, for example, taxa areal changes, vegetation dynamics, human impacts (e.g., deforestation), and climate change at global or continental scales. The harmonized pollen and metadata as well as the harmonization table are available from PANGAEA (https://doi.org/10.1594/PANGAEA.929773; Herzschuh et al., 2021). R code for the harmonization is provided at Zenodo (https://doi.org/10.5281/zenodo.5910972; Herzschuh et al., 2022) so that datasets at a customized harmonization level can be easily established.},
  language  = {en}
}
@article{KaunathEccard2022,
  author    = {Kaunath, Vera and Eccard, Jana},
  title     = {Light Attraction in Carabid Beetles},
  series = {Frontiers in Ecology and Evolution},
  volume    = {10},
  journal   = {Frontiers in Ecology and Evolution},
  publisher = {Frontiers Media},
  address   = {Lausanne, Schweiz},
  issn      = {2296-701X},
  doi       = {10.3389/fevo.2022.751288},
  pages     = {10},
  year      = {2022},
  abstract  = {Artificial light at night (ALAN) is altering the behaviour of nocturnal animals in a manifold of ways. Nocturnal invertebrates are particularly affected, due to their fatal attraction to ALAN. This selective pressure has the potential to reduce the strength of the flight-to-light response in insects, as shown recently in a moth species. Here we investigated light attraction of ground beetles (Coleoptera: Carabidae).We compared among animals (three genera) from a highly light polluted (HLP) grassland in the centre of Berlin and animals collected at a low-polluted area in a Dark Sky Reserve (DSR), captured using odour bait. In an arena setting tested at night time, HLP beetles (n = 75 across all genera) showed a reduced attraction towards ALAN. Tested during daytime, HLP beetles were less active in an open field test (measured as latency to start moving), compared to DSR (n = 143). However, we did not observe a reduced attraction towards ALAN within the species most common at both sides, Calathus fuscipes (HLP = 37, DSR = 118 individuals) indicating that not all species may be equally affected by ALAN. Reduced attraction to ALAN in urban beetles may either be a result of phenotypic selection in each generation removing HLP individuals that are attracted to light, or an indication for ongoing evolutionary differentiation among city and rural populations in their light response. Reduced attraction to light sources may directly enhance survival and reproductive success of urban individuals. However, decrease in mobility may negatively influence dispersal, reproduction and foraging success, highlighting the selective pressure that light pollution may have on fitness, by shaping and modifying the behaviour of insects.},
  language  = {en}
}
@article{SinghCompartALRawietal.2022,
  author    = {Singh, Aakanksha and Compart, Julia and AL-Rawi, Shadha Abduljaleel and Mahto, Harendra and Ahmad, Abubakar Musa and Fettke, J{\"o}rg},
  title     = {LIKE EARLY STARVATION 1 alters the glucan structures at the starch granule surface and thereby influences the action of both starch-synthesizing and starch-degrading enzymes},
  series = {The plant journal},
  volume    = {111},
  journal   = {The plant journal},
  number    = {3},
  publisher = {Wiley-Blackwell},
  address   = {Oxford},
  issn      = {0960-7412},
  doi       = {10.1111/tpj.15855},
  pages     = {819 -- 835},
  year      = {2022},
  abstract  = {For starch metabolism to take place correctly, various enzymes and proteins acting on the starch granule surface are crucial. Recently, two non-catalytic starch-binding proteins, pivotal for normal starch turnover in Arabidopsis leaves, namely, EARLY STARVATION 1 (ESV1) and its homolog LIKE EARLY STARVATION 1 (LESV), have been identified. Both share nearly 38\% sequence homology. As ESV1 has been found to influence glucan phosphorylation via two starch-related dikinases, alpha-glucan, water dikinase (GWD) and phosphoglucan, water dikinase (PWD), through modulating the surface glucan structures of the starch granules and thus affecting starch degradation, we assess the impact of its homolog LESV on starch metabolism. Thus, the 65-kDa recombinant protein LESV and the 50-kDa ESV1 were analyzed regarding their influence on the action of GWD and PWD on the surface of the starch granules. We included starches from various sources and additionally assessed the effect of these non-enzymatic proteins on other starch-related enzymes, such as starch synthases (SSI and SSIII), starch phosphorylases (PHS1), isoamylase and beta-amylase. The data obtained indicate that starch phosphorylation, hydrolyses and synthesis were affected by LESV and ESV1. Furthermore, incubation with LESV and ESV1 together exerted an additive effect on starch phosphorylation. In addition, a stable alteration of the glucan structures at the starch granule surface following treatment with LESV and ESV1 was observed. Here, we discuss all the observed changes that point to modifications in the glucan structures at the surface of the native starch granules and present a model to explain the existing processes.},
  language  = {en}
}
@article{MannaZoccaratoBanchietal.2022,
  author    = {Manna, Vincenzo and Zoccarato, Luca and Banchi, Elisa and Arnosti, Carol and Grossart, Hans-Peter and Celussi, Mauro},
  title     = {Linking lifestyle and foraging strategies of marine bacteria},
  series = {Environmental microbiology reports},
  volume    = {14},
  journal   = {Environmental microbiology reports},
  number    = {4},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {1758-2229},
  doi       = {10.1111/1758-2229.13059},
  pages     = {549 -- 558},
  year      = {2022},
  abstract  = {Microbe-mediated enzymatic hydrolysis of organic matter entails the production of hydrolysate, the recovery of which may be more or less efficient. The selfish uptake mechanism, recently discovered, allows microbes to hydrolyze polysaccharides and take up large oligomers, which are then degraded in the periplasmic space. By minimizing the hydrolysate loss, selfish behaviour may be profitable for free-living cells dwelling in a patchy substrate landscape. However, selfish uptake seems to be tailored to algal-derived polysaccharides, abundant in organic particles, suggesting that particle-attached microbes may use this strategy. We tracked selfish polysaccharides uptake in surface microbial communities of the northeastern Mediterranean Sea, linking the occurrence of this processing mode with microbial lifestyle. Additionally, we set up fluorescently labelled polysaccharides incubations supplying phytodetritus to investigate a 'pioneer' scenario for particle-attached microbes. Under both conditions, selfish behaviour was almost exclusively carried out by particle-attached microbes, suggesting that this mechanism may represent an advantage in the race for particle exploitation. Our findings shed light on the selfish potential of particle-attached microbes, suggesting multifaceted foraging strategies exerted by particle colonizers.},
  language  = {en}
}
@article{MummSchefflerHermanussen2022,
  author    = {Mumm, Rebekka and Scheffler, Christiane and Hermanussen, Michael},
  title     = {Locally structured correlation (LSC) plots describe inhomogeneity in normally distributed correlated bivariate variables},
  series = {Archives of Public Health},
  volume    = {80},
  journal   = {Archives of Public Health},
  publisher = {Springer Nature BMC},
  address   = {Bruxelles},
  issn      = {0778-7367},
  doi       = {10.1186/s13690-021-00748-4},
  pages     = {6},
  year      = {2022},
  abstract  = {Background The association between bivariate variables may not necessarily be homogeneous throughout the whole range of the variables. We present a new technique to describe inhomogeneity in the association of bivariate variables. Methods We consider the correlation of two normally distributed random variables. The 45° diagonal through the origin of coordinates represents the line on which all points would lie if the two variables completely agreed. If the two variables do not completely agree, the points will scatter on both sides of the diagonal and form a cloud. In case of a high association between the variables, the band width of this cloud will be narrow, in case of a low association, the band width will be wide. The band width directly relates to the magnitude of the correlation coefficient. We then determine the Euclidean distances between the diagonal and each point of the bivariate correlation, and rotate the coordinate system clockwise by 45°. The standard deviation of all Euclidean distances, named "global standard deviation", reflects the band width of all points along the former diagonal. Calculating moving averages of the standard deviation along the former diagonal results in "locally structured standard deviations" and reflect patterns of "locally structured correlations (LSC)". LSC highlight inhomogeneity of bivariate correlations. We exemplify this technique by analyzing the association between body mass index (BMI) and hip circumference (HC) in 6313 healthy East German adults aged 18 to 70 years. Results The correlation between BMI and HC in healthy adults is not homogeneous. LSC is able to identify regions where the predictive power of the bivariate correlation between BMI and HC increases or decreases, and highlights in our example that slim people have a higher association between BMI and HC than obese people. Conclusion Locally structured correlations (LSC) identify regions of higher or lower than average correlation between two normally distributed variables.},
  language  = {en}
}
@article{MummSchefflerHermanussen2022,
  author    = {Mumm, Rebekka and Scheffler, Christiane and Hermanussen, Michael},
  title     = {Locally structured correlation (LSC) plots describe inhomogeneity in normally distributed correlated bivariate variables},
  series = {Archives of public health = Archives belges de sant{\´e} publique = Belgisch archief voor volksgezondheid},
  volume    = {80},
  journal   = {Archives of public health = Archives belges de sant{\´e} publique = Belgisch archief voor volksgezondheid},
  number    = {1},
  publisher = {BMC},
  address   = {London},
  issn      = {0778-7367},
  doi       = {10.1186/s13690-021-00748-4},
  pages     = {6},
  year      = {2022},
  abstract  = {Background The association between bivariate variables may not necessarily be homogeneous throughout the whole range of the variables. We present a new technique to describe inhomogeneity in the association of bivariate variables. Methods We consider the correlation of two normally distributed random variables. The 45 degrees diagonal through the origin of coordinates represents the line on which all points would lie if the two variables completely agreed. If the two variables do not completely agree, the points will scatter on both sides of the diagonal and form a cloud. In case of a high association between the variables, the band width of this cloud will be narrow, in case of a low association, the band width will be wide. The band width directly relates to the magnitude of the correlation coefficient. We then determine the Euclidean distances between the diagonal and each point of the bivariate correlation, and rotate the coordinate system clockwise by 45 degrees. The standard deviation of all Euclidean distances, named "global standard deviation", reflects the band width of all points along the former diagonal. Calculating moving averages of the standard deviation along the former diagonal results in "locally structured standard deviations" and reflect patterns of "locally structured correlations (LSC)". LSC highlight inhomogeneity of bivariate correlations. We exemplify this technique by analyzing the association between body mass index (BMI) and hip circumference (HC) in 6313 healthy East German adults aged 18 to 70 years. Results The correlation between BMI and HC in healthy adults is not homogeneous. LSC is able to identify regions where the predictive power of the bivariate correlation between BMI and HC increases or decreases, and highlights in our example that slim people have a higher association between BMI and HC than obese people. Conclusion Locally structured correlations (LSC) identify regions of higher or lower than average correlation between two normally distributed variables.},
  language  = {en}
}
@article{CaoChenTianetal.2022,
  author    = {Cao, Xianyong and Chen, Jianhui and Tian, Fang and Xu, Qinghai and Herzschuh, Ulrike and Telford, Richard and Huang, Xiaozhong and Zheng, Zhuo and Shen, Caiming and Li, Wenjia},
  title     = {Long-distance modern analogues bias results of pollen-based precipitation reconstructions},
  series = {Science bulletin},
  volume    = {67},
  journal   = {Science bulletin},
  number    = {11},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {2095-9273},
  doi       = {10.1016/j.scib.2022.01.003},
  pages     = {1115 -- 1117},
  year      = {2022},
  language  = {en}
}
@article{LischeidWebberSommeretal.2022,
  author    = {Lischeid, Gunnar and Webber, Heidi and Sommer, Michael and Nendel, Claas and Ewert, Frank},
  title     = {Machine learning in crop yield modelling},
  series = {Agricultural and forest meteorology},
  volume    = {312},
  journal   = {Agricultural and forest meteorology},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0168-1923},
  doi       = {10.1016/j.agrformet.2021.108698},
  pages     = {23},
  year      = {2022},
  abstract  = {Provisioning a sufficient stable source of food requires sound knowledge about current and upcoming threats to agricultural production. To that end machine learning approaches were used to identify the prevailing climatic and soil hydrological drivers of spatial and temporal yield variability of four crops, comprising 40 years yield data each from 351 counties in Germany. Effects of progress in agricultural management and breeding were subtracted from the data prior the machine learning modelling by fitting smooth non-linear trends to the 95th percentiles of observed yield data. An extensive feature selection approach was followed then to identify the most relevant predictors out of a large set of candidate predictors, comprising various soil and meteorological data. Particular emphasis was placed on studying the uniqueness of identified key predictors. Random Forest and Support Vector Machine models yielded similar although not identical results, capturing between 50\% and 70\% of the spatial and temporal variance of silage maize, winter barley, winter rapeseed and winter wheat yield. Equally good performance could be achieved with different sets of predictors. Thus identification of the most reliable models could not be based on the outcome of the model study only but required expert's judgement. Relationships between drivers and response often exhibited optimum curves, especially for summer air temperature and precipitation. In contrast, soil moisture clearly proved less relevant compared to meteorological drivers. In view of the expected climate change both excess precipitation and the excess heat effect deserve more attention in breeding as well as in crop modelling.},
  language  = {en}
}