@article{BarazaNeserJacksonetal.2016,
  author    = {Baraza, Lilechi D. and Neser, Wekesa and Jackson, Korir Cheruiyot and Fredrick, Juma B. and Dennis, Ochieno and Wairimu, Kamau R. and Keya, Aggrey Osogo and Heydenreich, Matthias},
  title     = {Antimicrobial Coumarins from the Oyster Culinary-Medicinal Mushroom, Pleurotus ostreatus (Agaricomycetes), from Kenya},
  series = {International journal of medicinal mushrooms},
  volume    = {18},
  journal   = {International journal of medicinal mushrooms},
  publisher = {Begell House},
  address   = {Danbury},
  issn      = {1521-9437},
  doi       = {10.1615/IntJMedMushrooms.v18.i10.60},
  pages     = {905 -- 913},
  year      = {2016},
  abstract  = {Pleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. These have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. There are, however, few reports on the antimicrobial activities of different classes of purified compounds from P. ostreatus. This led to the current study, the objective of which was to chemically characterize the antibiotic activities of P. ()streams against selected human pathogenic bacteria and endophytic fungi. Chemical structures were determined using spectroscopic methods and by comparison with values of related structures reported in the literature. Pure compounds from P. ostreatus were tested in vitro against pathogenic bacteria (Staphylococcus aureus and Escherichia coli) and endophytic fungi (Pencillium digitatum and Fusarium prolferatum). A new compound, (E)-5,7-dimethoxy-6-(3-methylbuta-1,3-dienyl)-2H-chromen-2-one (5-methoxy-(E)-suberodiene) (compound 2), along with ergosterol (compound I.) and 5,7-dimethoxy-6-(3-methylbut-2-enyl)-2H-chromen-2-one (toddaculin; compound 3), were isolated from the fruiting bodies of P. ostreatus. The growth of S. aureus,E proliferatum, and P. digitatum colonies was inhibited in media containing compound 2, with minimum inhibitory concentrations closely comparable to those of conventional antibiotics.},
  language  = {en}
}
@article{GrunzelPilarekSteinbruecketal.2014,
  author    = {Grunzel, Petra and Pilarek, Maciej and Steinbrueck, Doerte and Neubauer, Antje and Brand, Eva and Kumke, Michael Uwe and Neubauer, Peter and Krause, Mirja},
  title     = {Mini-scale cultivation method enables expeditious plasmid production in Escherichia coli},
  series = {Biotechnology journal : systems \& synthetic biology, nanobiotech, medicine},
  volume    = {9},
  journal   = {Biotechnology journal : systems \& synthetic biology, nanobiotech, medicine},
  number    = {1},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1860-6768},
  doi       = {10.1002/biot.201300177},
  pages     = {128 -- 136},
  year      = {2014},
  abstract  = {The standard procedure in the lab for plasmid isolation usually involves a 2-mL, 16 h over-night cultivation in 15-mL bioreaction tubes in LB medium. This is time consuming, and not suitable for high-throughput applications. This study shows that it is possible to produce plasmid DNA (pDNA) in a 1.5-mL microcentrifuge tube with only 100 L cultivation volume in less than 7 h with a simple protocol. Compared with the standard LB cultivation for pDNA production reaching a final pDNA concentration range of 1.5-4 mu g mL(-1), a 6- to 10-fold increase in plasmid concentration (from 10 up to 25 mu g mL(-1) cultivation volume) is achieved using an optimized medium with an internal substrate delivery system (EnBase (R)). Different strains, plasmids, and the applicability of different inoculation tools (i.e. different starting ODs) were compared, demonstrating the robustness of the system. Additionally, dissolved oxygen was monitored in real time online, indicating that under optimized conditions oxygen limitation can be avoided. We developed a simple protocol with a significantly decreased procedure time, enabling simultaneous handling of more samples, while a consistent quality and a higher final pDNA concentration are ensured.},
  language  = {en}
}