@article{KienzlerFlehrGehneetal.2012,
  author    = {Kienzler, Andrea Altevogt Nee and Flehr, Roman and Gehne, S{\"o}ren and Kumke, Michael Uwe and Bannwarth, Willi},
  title     = {Verification and biophysical characterization of a New Three-Color Forster Resonance-Energy-Transfer (FRET) System in DNA},
  series = {Helvetica chimica acta},
  volume    = {95},
  journal   = {Helvetica chimica acta},
  number    = {4},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0018-019X},
  doi       = {10.1002/hlca.201100460},
  pages     = {543 -- 555},
  year      = {2012},
  abstract  = {We report on a new three-color FRET system consisting of three fluorescent dyes, i.e., of a carbostyril (=quinolin-2(1H)-one)-derived donor D, a (bathophenanthroline)ruthenium complex as a relay chromophore A1, and a Cy dye as A2 (FRET=Forster resonance-energy-transfer) (cf. Fig. 1). With their widely matching spectroscopic properties (cf. Fig. 2), the combination of these dyes yielded excellent FRET efficiencies. Furthermore, fluorescence lifetime measurements revealed that the long fluorescence lifetime of the Ru complex was transferred to the Cy dye offering the possibility to measure the whole system in a time-resolved mode. The FRET system was established on double-stranded DNA (cf. Fig. 3) but it should also be generally applicable to other biomolecules.},
  language  = {en}
}