@article{HaferKiyLucke2014, author = {Hafer, J{\"o}rg and Kiy, Alexander and Lucke, Ulrike}, title = {Moodle \& Co. auf dem Weg zur Personal Learning Environment}, series = {eleed}, volume = {2014}, journal = {eleed}, number = {10}, issn = {1860-7470}, year = {2014}, abstract = {Ausgehend von der typischen IT-Infrastruktur f{\"u}r E-Learning an Hochschulen auf der einen Seite sowie vom bisherigen Stand der Forschung zu Personal Learning Environments (PLEs) auf der anderen Seite zeigt dieser Beitrag auf, wie bestehende Werkzeuge bzw. Dienste zusammengef{\"u}hrt und f{\"u}r die Anforderungen der modernen, rechnergest{\"u}tzten Pr{\"a}senzlehre aufbereitet werden k{\"o}nnen. F{\"u}r diesen interdisziplin{\"a}ren Entwicklungsprozess bieten sowohl klassische Softwareentwicklungsverfahren als auch bestehende PLE-Modelle wenig Hilfestellung an. Der Beitrag beschreibt die in einem campusweiten Projekt an der Universit{\"a}t Potsdam verfolgten Ans{\"a}tze und die damit erzielten Ergebnisse. Daf{\"u}r werden zun{\"a}chst typische Lehr-/Lern-bzw. Kommunikations-Szenarien identifiziert, aus denen Anforderungen an eine unterst{\"u}tzende Plattform abgeleitet werden. Dies f{\"u}hrt zu einer umfassenden Sammlung zu ber{\"u}cksichtigender Dienste und deren Funktionen, die gem{\"a}ß den Spezifika ihrer Nutzung in ein Gesamtsystem zu integrieren sind. Auf dieser Basis werden grunds{\"a}tzliche Integrationsans{\"a}tze und technische Details dieses Mash-Ups in einer Gesamtschau aller relevanten Dienste betrachtet und in eine integrierende Systemarchitektur {\"u}berf{\"u}hrt. Deren konkrete Realisierung mit Hilfe der Portal-Technologie Liferay wird dargestellt, wobei die eingangs definierten Szenarien aufgegriffen und exemplarisch vorgestellt werden. Erg{\"a}nzende Anpassungen im Sinne einer personalisierbaren bzw. adaptiven Lern-(und Arbeits-)Umgebung werden ebenfalls unterst{\"u}tzt und kurz aufgezeigt.}, language = {en} } @article{BoggioBodenmuellerFrembergetal.2014, author = {Boggio, Jose M. Chavez and Bodenmueller, D. and Fremberg, T. and Haynes, R. and Roth, Martin M. and Eisermann, R. and Lisker, M. and Zimmermann, L. and Boehm, Michael}, title = {Dispersion engineered silicon nitride waveguides by geometrical and refractive-index optimization}, series = {Journal of the Optical Society of America : B, Optical physics}, volume = {31}, journal = {Journal of the Optical Society of America : B, Optical physics}, number = {11}, publisher = {Optical Society of America}, address = {Washington}, issn = {0740-3224}, doi = {10.1364/JOSAB.31.002846}, pages = {2846 -- 2857}, year = {2014}, abstract = {Dispersion engineering in silicon nitride (SiXNY) waveguides is investigated through the optimization of the waveguide transversal dimensions and refractive indices in a multicladding arrangement. Ultraflat dispersion of -84.0 +/- 0.5 ps/nm/km between 1700 and 2440 nm and 1.5 +/- 3 ps/nm/km between 1670 and 2500 nm is numerically demonstrated. It is shown that typical refractive index fluctuations as well as dimension fluctuations during fabrication of the SiXNY waveguides are a limitation for obtaining ultraflat dispersion profiles. Single- and multicladding waveguides are fabricated and their dispersion profiles measured (over nearly 1000 nm) using a low-coherence frequency domain interferometric technique. By appropriate thickness optimization, the zero-dispersion wavelength is tuned over a large spectral range in single-and multicladding waveguides with small refractive index contrast (3\%). A flat dispersion profile with +/- 3.2 ps/nm/km variation over 500 nm is obtained in a multicladding waveguide fabricated with a refractive index contrast of 37\%. Finally, we generate a nearly three-octave supercontinuum in this dispersion flattened multicladding SiXNY waveguide. (C) 2014 Optical Society of America}, language = {en} } @article{SchmaelzlinMoralejoRutowskaetal.2014, author = {Schmaelzlin, Elmar and Moralejo, Benito and Rutowska, Monika and Monreal-Ibero, Ana and Sandin, Christer and Tarcea, Nicolae and Popp, Juergen and Roth, Martin M.}, title = {Raman imaging with a fiber-coupled multichannel spectrograph}, series = {Sensors}, volume = {14}, journal = {Sensors}, number = {11}, publisher = {MDPI}, address = {Basel}, issn = {1424-8220}, doi = {10.3390/s141121968}, pages = {21968 -- 21980}, year = {2014}, abstract = {Until now, spatially resolved Raman Spectroscopy has required to scan a sample under investigation in a time-consuming step-by-step procedure. Here, we present a technique that allows the capture of an entire Raman image with only one single exposure. The Raman scattering arising from the sample was collected with a fiber-coupled high-performance astronomy spectrograph. The probe head consisting of an array of 20 x 20 multimode fibers was linked to the camera port of a microscope. To demonstrate the high potential of this new concept, Raman images of reference samples were recorded. Entire chemical maps were received without the need for a scanning procedure.}, language = {en} } @article{BeisheimLiese2014, author = {Beisheim, Marianne and Liese, Andrea Margit}, title = {Summing up : key findings and avenues for future research}, isbn = {978-1-137-35925-0}, year = {2014}, language = {en} } @article{SultanowWeber2014, author = {Sultanow, Eldar and Weber, Edzard}, title = {Prozessleitst{\"a}nde f{\"u}r verteilte und nichtplanbare Organisationsprozesse}, series = {Handbuch prozessorientiertes Wissensmanagement}, journal = {Handbuch prozessorientiertes Wissensmanagement}, publisher = {GITO}, address = {Berlin}, isbn = {978-3-95545-026-7}, pages = {335 -- 344}, year = {2014}, language = {de} } @article{VladovaBahrs2014, author = {Vladova, Gergana and Bahrs, Julian}, title = {Wissenstransfer als Mittel der internen Unternehmenskommunikation}, series = {Handbuch prozessorientiertes Wissensmanagment}, journal = {Handbuch prozessorientiertes Wissensmanagment}, publisher = {GITO}, address = {Berlin}, isbn = {978-3-95545-026-7}, pages = {37 -- 47}, year = {2014}, language = {de} } @article{Apelojg2014, author = {Apelojg, Benjamin}, title = {Innovationen-Kreativit{\"a}t-Schule}, series = {Wissenschaft - Innovation- Technologie}, volume = {37}, journal = {Wissenschaft - Innovation- Technologie}, editor = {Banse, Gerhard and Grimmeis, Hermann}, publisher = {Trafo-Verlag}, address = {Berlin}, isbn = {978-3864640537}, pages = {325 -- 340}, year = {2014}, language = {de} } @article{BadalyanDierichStibaetal.2014, author = {Badalyan, Artavazd and Dierich, Marlen and Stiba, Konstanze and Schwuchow, Viola and Leimk{\"u}hler, Silke and Wollenberger, Ulla}, title = {Electrical wiring of the aldehyde oxidoreductase PaoABC with a polymer containing osmium redox centers}, series = {Biosensors}, volume = {4}, journal = {Biosensors}, number = {4}, publisher = {MDPI}, address = {Basel}, doi = {10.3390/bios4040403}, pages = {403 -- 421}, year = {2014}, abstract = {Biosensors for the detection of benzaldehyde and g-aminobutyric acid (GABA) are reported using aldehyde oxidoreductase PaoABC from Escherichia coli immobilized in a polymer containing bound low potential osmium redox complexes. The electrically connected enzyme already electrooxidizes benzaldehyde at potentials below -0.15 V (vs. Ag|AgCl, 1 M KCl). The pH-dependence of benzaldehyde oxidation can be strongly influenced by the ionic strength. The effect is similar with the soluble osmium redox complex and therefore indicates a clear electrostatic effect on the bioelectrocatalytic efficiency of PaoABC in the osmium containing redox polymer. At lower ionic strength, the pH-optimum is high and can be switched to low pH-values at high ionic strength. This offers biosensing at high and low pH-values. A "reagentless" biosensor has been formed with enzyme wired onto a screen-printed electrode in a flow cell device. The response time to addition of benzaldehyde is 30 s, and the measuring range is between 10-150 µM and the detection limit of 5 µM (signal to noise ratio 3:1) of benzaldehyde. The relative standard deviation in a series (n = 13) for 200 µM benzaldehyde is 1.9\%. For the biosensor, a response to succinic semialdehyde was also identified. Based on this response and the ability to work at high pH a biosensor for GABA is proposed by coimmobilizing GABA-aminotransferase (GABA-T) and PaoABC in the osmium containing redox polymer.}, language = {en} } @article{FritzschWangdosSantosetal.2014, author = {Fritzsch, Claire and Wang, Jing and dos Santos, Luara Ferreira and Mauritz, Karl-Heinz and Brunetti, Maddalena and Dohle, Christian}, title = {Different effects of the mirror illusion on motor and somatosensory processing}, series = {Restorative neurology and neuroscience}, volume = {32}, journal = {Restorative neurology and neuroscience}, number = {2}, publisher = {IOS Press}, address = {Amsterdam}, issn = {0922-6028}, doi = {10.3233/RNN-130343}, pages = {269 -- 280}, year = {2014}, abstract = {Purpose: Mirror therapy can improve motor and sensory functions, but effects of the mirror illusion on primary motor and somatosensory cortex could not be established consistently. Methods: Fifteen right handed healthy volunteers performed or observed a finger-thumb opposition task. Cerebral activations during normal movement (NOR), mirrored movement (MIR) and movement observation (OBS) by means of a video chain were recorded with functional magnetic resonance imaging (fMRI). Activation sizes in movement > static conditions were identified using SPM8 (p < 0.001, unc.) and attributed to predefined areas employing the Anatomy toolbox 1.8. Laterality indices for the responsive areas were calculated on the basis of the number of activated voxels. Results: Relevant bilateral BOLD responses were found in primary motor (M1) and somatosensory (S1 - BA 2, 3b and 3a) cortex, premotor and parietal areas and V5. When comparing MIR to NOR, no significant change of contralateral activation in M1 was found, but clearly at S1 with differences between hands. Conclusion: The mirror illusion does not elicit immediate changes in motor areas, yet there is a direct effect on somatosensory areas, especially for left hand movements. These results suggest different effects of mirror therapy on processing and rehabilitation of motor and sensory function.}, language = {en} } @article{Wyrwa2014, author = {Wyrwa, Ulrich}, title = {For the Hundredth nothing New German Language New Releases to the First World War (Part I)}, series = {Deutsche Zeitschrift f{\"u}r Geschichtswissenschaft}, volume = {62}, journal = {Deutsche Zeitschrift f{\"u}r Geschichtswissenschaft}, number = {11}, publisher = {Metropol-Verl.}, address = {Berlin}, issn = {0044-2828}, pages = {921 -- 940}, year = {2014}, language = {de} } @article{Crome2014, author = {Crome, Erhard}, title = {Deutschland und die USA : eine geopolitische Perspektive}, year = {2014}, language = {de} } @article{Roeder2014, author = {R{\"o}der, Katrin}, title = {Reparative reading, post-structuralist Hermeneutics and T. S. Eliot's four quartets}, series = {Anglia : journal of English philology}, volume = {132}, journal = {Anglia : journal of English philology}, number = {1}, publisher = {De Gruyter}, address = {Berlin}, issn = {0340-5222}, doi = {10.1515/anglia-2014-0004}, pages = {58 -- 77}, year = {2014}, abstract = {This essay approaches T. S. Eliot's Four Quartets (1935-1942) from the perspectives of Eve Kosofsky Sedgwick's critical practice of reparative reading and of Paul Ricoeur's poststructuralist hermeneutics. It demonstrates that Sedgwick's and Ricoeur's approaches can be productively combined to investigate hermeneutic processes in which the textual energy of a dissemination of meaning is redirected by a reparative or integrative impulse. In Four Quartets, this impetus induces the creation of semantic innovation through a violation of semantic pertinence, that is, through novel, tensional and provisional connections between formerly separate textual elements and semantic units.}, language = {en} } @article{DolgenerFreudenbergerSchneeweissetal.2014, author = {Dolgener, Nicola and Freudenberger, L. and Schneeweiss, N. and Ibisch, P. L. and Tiedemann, Ralph}, title = {Projecting current and potential future distribution of the Fire-bellied toad Bombina bombina under climate change in north-eastern Germany}, series = {Regional environmental change}, volume = {14}, journal = {Regional environmental change}, number = {3}, publisher = {Springer}, address = {Heidelberg}, issn = {1436-3798}, doi = {10.1007/s10113-013-0468-9}, pages = {1063 -- 1072}, year = {2014}, abstract = {Environmental change is likely to have a strong impact on biodiversity, and many species may shift their distribution in response. In this study, we aimed at projecting the availability of suitable habitat for an endangered amphibian species, the Fire-bellied toad Bombina bombina, in Brandenburg (north-eastern Germany). We modelled a potential habitat distribution map based on (1) a database with 10,581 presence records for Bombina from the years 1990 to 2009, (2) current estimates for ecogeographical variables (EGVs) and (3) the future projection of these EGVs according to the statistical regional model, respectively, the soil and water integrated model, applying the maximum entropy approach (Maxent). By comparing current and potential future distributions, we evaluated the projected change in distribution of suitable habitats and identified the environmental variables most associated with habitat suitability that turned out to be climatic variables related to the hydrological cycle. Under the applied scenario, our results indicate increasing habitat suitability in many areas and an extended range of suitable habitats. However, even if the environmental conditions in Brandenburg may change as predicted, it is questionable whether the Fire-bellied toad will truly benefit, as dispersal abilities of amphibian species are limited and strongly influenced by anthropogenic disturbances, that is, intensive agriculture, habitat destruction and fragmentation. Furthermore, agronomic pressure is likely to increase on productive areas with fertile soils and high water retention capacities, indeed those areas suitable for B. bombina. All these changes may affect temporary pond hydrology as well as the reproductive success and breeding phenology of toads.}, language = {en} } @article{DolgenerFreudenbergerSchlucketal.2014, author = {Dolgener, N. and Freudenberger, L. and Schluck, M. and Schneeweiss, N. and Ibisch, P. L. and Tiedemann, Ralph}, title = {Environmental niche factor analysis (ENFA) relates environmental parameters to abundance and genetic diversity in an endangered amphibian, the fire-bellied-toad (Bombina bombina)}, series = {Conservation genetics}, volume = {15}, journal = {Conservation genetics}, number = {1}, publisher = {Springer}, address = {Dordrecht}, issn = {1566-0621}, doi = {10.1007/s10592-013-0517-4}, pages = {11 -- 21}, year = {2014}, abstract = {Increasing attempts are made to understand the factors responsible for both the demographic and genetic depletion in amphibian populations. Landscape genetics aims at a spatially explicit correlation of genetic population parameters to landscape features. Using data from the endangered fire-bellied toad Bombina bombina in Brandenburg (Northeastern Germany), we performed an environmental niche factor analysis (ENFA), relating demographic (abundance) and genetic (diversity at 17 microsatellite loci and partial sequences of the mitochondrial control region in 434 individuals from 16 populations) parameters to ecological and anthropogenic variables such as temperature, precipitation, soil wetness, water runoff, vegetation density, and road/traffic impact. We found significant correlations between road disturbance and observed heterozygosity and between soil wetness and mitochondrial diversity. As the influences of the environmental variables can differ between different indicators for genetic diversity, population size and abundance data, our ENFA-based landscape genetics approach allows us to put various aspects of long- versus short term effective population size and genetic connectivity into an ecological and spatially explicit context, enabling potentially even forecast assessment under future environmental scenarios.}, language = {en} } @article{TiedemannSchneiderHavensteinetal.2014, author = {Tiedemann, Ralph and Schneider, Anja R. R. and Havenstein, Katja and Blanck, Torsten and Meier, Elmar and Raffel, Martina and Zwartepoorte, Henk and Plath, Martin}, title = {New microsatellite markers allow high-resolution taxon delimitation in critically endangered Asian box turtles, genus Cuora}, series = {Salamandra : German journal of herpetology}, volume = {50}, journal = {Salamandra : German journal of herpetology}, number = {3}, publisher = {Deutsche Gesellschaft f{\"u}r Herpetologie und Terrarienkunde}, address = {Darmstadt}, issn = {0036-3375}, pages = {139 -- 146}, year = {2014}, abstract = {We isolated and characterized 16 new di- and tetranudeotide microsatellite markers for the critically endangered Asian box turtle genus Cuora, focusing on the "Cuora trifasciata" species complex. The new markers were then used to analyse genetic variability and divergence amongst five described species within this complex, namely C. aurocapitata (n = 18), C. cyclornata (n = 31), C. pani (n = 6), C. trifasciata (n = 58), and C. zhoui (n = 7). Our results support the view that all five species represent valid taxa. Within two species (C. trifasciata and C. cyclornata), two distinct morphotypes were corroborated by microsatellite divergence. For three individuals, morphologically identified as being of hybrid origin, the hybrid status was confirmed by our genetic analysis. Our results confirm the controversial species (Cuora aurocapitata, C. cyclornata) and subspecies/morphotypes (C. cyclornata meieri, C. trifasciata cf. trifasciata) to be genetically distinct, which has critical implications for conservation strategies.}, language = {en} } @article{Ippolito2014, author = {Ippolito, Antonella}, title = {L' Inferno nel ciclo dei Contorni danteschi di Bonaventura Genelli}, series = {Dante e l' Arte}, volume = {2014}, journal = {Dante e l' Arte}, number = {1}, publisher = {Univ. Aut{\`o}noma de Barcelona}, address = {Bellaterra}, issn = {2385-5355}, pages = {241 -- 270}, year = {2014}, abstract = {Il ciclo dei trentasei Contorni danteschi del pittore e disegnatore berlinese Giovanni Bonaventura Genelli (1798-1868) rappresenta un interessante tentativo d'interpretare il testo della Commedia alla luce dei principi del classicismo tedesco, sull'esempio di Carstens e di Koch e, in parte di Flaxman da cui per{\`o} Genelli si distingue per continua ricerca volta ad esprimere la plasticit{\`a} delle forme. Caratteristica precipua delle sue illustrazioni {\`e} inoltre il ricorso continuo sia alla scultura antica che agli affreschi michelangioleschi della Sistina come repertorio iconografico. L'articolo approfondisce questi aspetti con particolare riferimento alle sedici tavole dedicate all'Inferno.}, language = {it} } @article{WessigGerngrossPapeetal.2014, author = {Wessig, Pablo and Gerngroß, Maik and Pape, Simon and Bruhns, Philipp and Weber, Jens}, title = {Novel porous materials based on oligospiroketals (OSK)}, series = {RSC Advances : an international journal to further the chemical sciences}, volume = {2014}, journal = {RSC Advances : an international journal to further the chemical sciences}, number = {4}, issn = {2046-2069}, doi = {10.1039/c4ra04437a}, pages = {31123 -- 31129}, year = {2014}, abstract = {New porous materials based on covalently connected monomers are presented. The key step of the synthesis is an acetalisation reaction. In previous years we used acetalisation reactions extensively to build up various molecular rods. Based on this approach, investigations towards porous polymeric materials were conducted by us. Here we wish to present the results of these studies in the synthesis of 1D polyacetals and porous 3D polyacetals. By scrambling experiments with 1D acetals we could prove that exchange reactions occur between different building blocks (evidenced by MALDI-TOF mass spectrometry). Based on these results we synthesized porous 3D polyacetals under the same mild conditions.}, language = {en} } @article{ZamponiPenfoldNachtegaaletal.2014, author = {Zamponi, Flavio and Penfold, Thomas J. and Nachtegaal, Maarten and L{\"u}bcke, Andrea and Rittmann, Jochen and Milne, Chris J. and Chergui, Majed and van Bokhoven, Jeroen A.}, title = {Probing the dynamics of plasmon-excited hexanethiol-capped gold nanoparticles by picosecond X-ray absorption spectroscopy}, series = {physical chemistry, chemical physics : PCCP}, volume = {2014}, journal = {physical chemistry, chemical physics : PCCP}, number = {16}, issn = {1463-9076}, doi = {10.1039/c4cp03301a}, pages = {23157 -- 23163}, year = {2014}, abstract = {Picosecond X-ray absorption spectroscopy (XAS) is used to investigate the electronic and structural dynamics initiated by plasmon excitation of 1.8 nm diameter Au nanoparticles (NPs) functionalised with 1-hexanethiol. We show that 100 ps after photoexcitation the transient XAS spectrum is consistent with an 8\% expansion of the Au-Au bond length and a large increase in disorder associated with melting of the NPs. Recovery of the ground state occurs with a time constant of ∼1.8 ns, arising from thermalisation with the environment. Simulations reveal that the transient spectrum exhibits no signature of charge separation at 100 ps and allows us to estimate an upper limit for the quantum yield (QY) of this process to be <0.1.}, language = {en} } @article{MeyerMatissekMuelleretal.2014, author = {Meyer, S{\"o}ren and Matissek, M. and M{\"u}ller, Sandra Marie and Taleshi, M. S. and Ebert, Franziska and Francesconi, Kevin A. and Schwerdtle, Tanja}, title = {In vitro toxicological characterisation of three arsenic-containing hydrocarbons}, series = {Metallomics}, volume = {2014}, journal = {Metallomics}, number = {6}, issn = {1756-591X}, doi = {10.1039/c4mt00061g}, pages = {1023 -- 1033}, year = {2014}, abstract = {Arsenic-containing hydrocarbons are one group of fat-soluble organic arsenic compounds (arsenolipids) found in marine fish and other seafood. A risk assessment of arsenolipids is urgently needed, but has not been possible because of the total lack of toxicological data. In this study the cellular toxicity of three arsenic-containing hydrocarbons was investigated in cultured human bladder (UROtsa) and liver (HepG2) cells. Cytotoxicity of the arsenic-containing hydrocarbons was comparable to that of arsenite, which was applied as the toxic reference arsenical. A large cellular accumulation of arsenic, as measured by ICP-MS/MS, was observed after incubation of both cell lines with the arsenolipids. Moreover, the toxic mode of action shown by the three arsenic-containing hydrocarbons seemed to differ from that observed for arsenite. Evidence suggests that the high cytotoxic potential of the lipophilic arsenicals results from a decrease in the cellular energy level. This first in vitro based risk assessment cannot exclude a risk to human health related to the presence of arsenolipids in seafood, and indicates the urgent need for further toxicity studies in experimental animals to fully assess this possible risk.}, language = {en} } @article{Groezinger2014, author = {Gr{\"o}zinger, Karl E.}, title = {J{\"u}dische Mystik}, series = {Handbuch zur Geschichte der Juden in Europa}, journal = {Handbuch zur Geschichte der Juden in Europa}, publisher = {Primus Verlag}, address = {Frankfurt a.M.}, isbn = {978-3-86312-363-5}, pages = {127 -- 137}, year = {2014}, abstract = {Das Handbuch umreißt das gesamte Spektrum der 2000j{\"a}hrigen Geschichte der Juden auf europ{\"a}ischem Boden. Wissenschaftler aus Europa und den USA haben ihre Forschungsergebnisse allgemein verst{\"a}ndlich aufbereitet mit dem Ziel, das Leben und Wirken der Juden, aber auch die ihnen entgegengebrachte Intoleranz und deren Ursachen aufzuzeigen. Der erste Teil konzentriert sich systematisch auf die L{\"a}nder und Regionen, in denen Juden siedelten bzw. nach Vertreibungen aus anderen Staaten Aufnahme fanden. Es wird sowohl auf die innere Entwicklung der j{\"u}dischen Gemeinden als auch auf die Beziehungen zwischen Juden und der sie umgebenden andersgl{\"a}ubigen Gesellschaft eingegangen. Der zweite Teil behandelt themenspezifische Schwerpunkte. Gemeindeleben und Religion, Familie und Stellung der Frau, kulturelle und geistige Entwicklung, aber auch Judenfeindschaft der sie umgebenden Gesellschaft - vom Antijudaismus bis zur Shoa und zum Antisemitismus der Gegenwart - werden in großangelegten Beitr{\"a}gen dargelegt.}, language = {de} } @article{UnterbergLeffersHuebneretal.2014, author = {Unterberg, Marlies and Leffers, Larissa and H{\"u}bner, Florian and Humpf, Hans-Ulrich and Lepikhov, Konstantin and Walter, J{\"o}rn and Ebert, Franziska and Schwerdtle, Tanja}, title = {Toxicity of arsenite and thio-DMAV after long-term (21 days) incubation of human urothelial cells: cytotoxicity, genotoxicity and epigenetics}, series = {Toxicology Research}, volume = {3}, journal = {Toxicology Research}, number = {6}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {2045-4538}, pages = {456 -- 464}, year = {2014}, abstract = {This study aims to further mechanistically understand toxic modes of action after chronic inorganic arsenic exposure. Therefore long-term incubation studies in cultured cells were carried out, to display chronically attained changes, which cannot be observed in the generally applied in vitro short-term incubation studies. Particularly, the cytotoxic, genotoxic and epigenetic effects of an up to 21 days incubation of human urothelial (UROtsa) cells with pico- to nanomolar concentrations of iAsIII and its metabolite thio-DMAV were compared. After 21 days of incubation, cytotoxic effects were strongly enhanced in the case of iAsIII and might partly be due to glutathione depletion and genotoxic effects on the chromosomal level. These results are in strong contrast to cells exposed to thio-DMAV. Thus, cells seemed to be able to adapt to this arsenical, as indicated among others by an increase in the cellular glutathione level. Most interestingly, picomolar concentrations of both iAsIII and thio-DMAV caused global DNA hypomethylation in UROtsa cells, which was quantified in parallel by 5-medC immunostaining and a newly established, reliable, high resolution mass spectrometry (HRMS)-based test system. This is the first time that epigenetic effects are reported for thio-DMAV; iAsIII induced epigenetic effects occur in at least 8000 fold lower concentrations as reported in vitro before. The fact that both arsenicals cause DNA hypomethylation at really low, exposure-relevant concentrations in human urothelial cells suggests that this epigenetic effect might contribute to inorganic arsenic induced carcinogenicity, which for sure has to be further investigated in future studies.}, language = {en} } @article{PalyulinAlaNissilaMetzler2014, author = {Palyulin, Vladimir V. and Ala-Nissila, Tapio and Metzler, Ralf}, title = {Polymer translocation: the first two decades and the recent diversification}, series = {Soft matter}, volume = {45}, journal = {Soft matter}, number = {10}, editor = {Metzler, Ralf}, publisher = {the Royal Society of Chemistry}, address = {Cambridge}, issn = {1744-683X}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-76266}, pages = {9016 -- 9037}, year = {2014}, abstract = {Probably no other field of statistical physics at the borderline of soft matter and biological physics has caused such a flurry of papers as polymer translocation since the 1994 landmark paper by Bezrukov, Vodyanoy, and Parsegian and the study of Kasianowicz in 1996. Experiments, simulations, and theoretical approaches are still contributing novel insights to date, while no universal consensus on the statistical understanding of polymer translocation has been reached. We here collect the published results, in particular, the famous-infamous debate on the scaling exponents governing the translocation process. We put these results into perspective and discuss where the field is going. In particular, we argue that the phenomenon of polymer translocation is non-universal and highly sensitive to the exact specifications of the models and experiments used towards its analysis.}, language = {en} } @article{Berner2014, author = {Berner, Elisabeth}, title = {Von der Schwierigkeit, einer Stadt eine Kunsthalle zu schenken}, series = {Emotionalit{\"a}t im Text}, journal = {Emotionalit{\"a}t im Text}, number = {85}, publisher = {Stauffenburg}, address = {T{\"u}bingen}, isbn = {978-3-95809-506-9}, issn = {1430-4139}, pages = {231 -- 248}, year = {2014}, language = {de} } @article{Teske2014, author = {Teske, Daniel}, title = {Geocoder accuracy ranking}, series = {Process design for natural scientists: an agile model-driven approach}, journal = {Process design for natural scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {161 -- 174}, year = {2014}, abstract = {Finding an address on a map is sometimes tricky: the chosen map application may be unfamiliar with the enclosed region. There are several geocoders on the market, they have different databases and algorithms to compute the query. Consequently, the geocoding results differ in their quality. Fortunately the geocoders provide a rich set of metadata. The workflow described in this paper compares this metadata with the aim to find out which geocoder is offering the best-fitting coordinate for a given address.}, language = {en} } @article{Sens2014, author = {Sens, Henriette}, title = {Web-Based map generalization tools put to the test: a jABC workflow}, series = {Process Design for Natural Scientists: an agile model-driven approach}, journal = {Process Design for Natural Scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {175 -- 185}, year = {2014}, abstract = {Geometric generalization is a fundamental concept in the digital mapping process. An increasing amount of spatial data is provided on the web as well as a range of tools to process it. This jABC workflow is used for the automatic testing of web-based generalization services like mapshaper.org by executing its functionality, overlaying both datasets before and after the transformation and displaying them visually in a .tif file. Mostly Web Services and command line tools are used to build an environment where ESRI shapefiles can be uploaded, processed through a chosen generalization service and finally visualized in Irfanview.}, language = {en} } @article{Noack2014, author = {Noack, Franziska}, title = {CREADED: Colored-Relief application for digital elevation data}, series = {Process design for natural scientists: an agile model-driven approach}, journal = {Process design for natural scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {186 -- 199}, year = {2014}, abstract = {In the geoinformatics field, remote sensing data is often used for analyzing the characteristics of the current investigation area. This includes DEMs, which are simple raster grids containing grey scales representing the respective elevation values. The project CREADED that is presented in this paper aims at making these monochrome raster images more significant and more intuitively interpretable. For this purpose, an executable interactive model for creating a colored and relief-shaded Digital Elevation Model (DEM) has been designed using the jABC framework. The process is based on standard jABC-SIBs and SIBs that provide specific GIS functions, which are available as Web services, command line tools and scripts.}, language = {en} } @article{Respondek2014, author = {Respondek, Tobias}, title = {A workflow for computing potential areas for wind turbines}, series = {Process design for natural scientists: an agile model-driven approach}, journal = {Process design for natural scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, pages = {200 -- 215}, year = {2014}, abstract = {This paper describes the implementation of a workflow model for service-oriented computing of potential areas for wind turbines in jABC. By implementing a re-executable model the manual effort of a multi-criteria site analysis can be reduced. The aim is to determine the shift of typical geoprocessing tools of geographic information systems (GIS) from the desktop to the web. The analysis is based on a vector data set and mainly uses web services of the "Center for Spatial Information Science and Systems" (CSISS). This paper discusses effort, benefits and problems associated with the use of the web services.}, language = {en} } @article{Scheele2014, author = {Scheele, Lasse}, title = {Location analysis for placing artificial reefs}, series = {Process design for natural scientists: an agile model-driven approach}, journal = {Process design for natural scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {216 -- 228}, year = {2014}, abstract = {Location analyses are among the most common tasks while working with spatial data and geographic information systems. Automating the most frequently used procedures is therefore an important aspect of improving their usability. In this context, this project aims to design and implement a workflow, providing some basic tools for a location analysis. For the implementation with jABC, the workflow was applied to the problem of finding a suitable location for placing an artificial reef. For this analysis three parameters (bathymetry, slope and grain size of the ground material) were taken into account, processed, and visualized with the The Generic Mapping Tools (GMT), which were integrated into the workflow as jETI-SIBs. The implemented workflow thereby showed that the approach to combine jABC with GMT resulted in an user-centric yet user-friendly tool with high-quality cartographic outputs.}, language = {en} } @article{Kind2014, author = {Kind, Josephine}, title = {Creation of topographic maps}, series = {Process design for natural scientists: an agile model-driven approach}, journal = {Process design for natural scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, pages = {229 -- 238}, year = {2014}, abstract = {Location analyses are among the most common tasks while working with spatial data and geographic information systems. Automating the most frequently used procedures is therefore an important aspect of improving their usability. In this context, this project aims to design and implement a workflow, providing some basic tools for a location analysis. For the implementation with jABC, the workflow was applied to the problem of finding a suitable location for placing an artificial reef. For this analysis three parameters (bathymetry, slope and grain size of the ground material) were taken into account, processed, and visualized with the The Generic Mapping Tools (GMT), which were integrated into the workflow as jETI-SIBs. The implemented workflow thereby showed that the approach to combine jABC with GMT resulted in an user-centric yet user-friendly tool with high-quality cartographic outputs.}, language = {en} } @article{Holler2014, author = {Holler, Robin}, title = {GraffDok - a graffiti documentation application}, series = {Process design for natural scientists: an agile model-driven approach}, journal = {Process design for natural scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {239 -- 251}, year = {2014}, abstract = {GraffDok is an application helping to maintain an overview over sprayed images somewhere in a city. At the time of writing it aims at vandalism rather than at beautiful photographic graffiti in an underpass. Looking at hundreds of tags and scribbles on monuments, house walls, etc. it would be interesting to not only record them in writing but even make them accessible electronically, including images. GraffDok's workflow is simple and only requires an EXIF-GPS-tagged photograph of a graffito. It automatically determines its location by using reverse geocoding with the given GPS-coordinates and the Gisgraphy WebService. While asking the user for some more meta data, GraffDok analyses the image in parallel with this and tries to detect fore- and background - before extracting the drawing lines and make them stand alone. The command line based tool ImageMagick is used here as well as for accessing EXIF data. Any meta data is written to csv-files, which will stay easily accessible and can be integrated in TeX-files as well. The latter ones are converted to PDF at the end of the workflow, containing a table about all graffiti and a summary for each - including the generated characteristic graffiti pattern image.}, language = {en} } @article{Kosta2014, author = {Kosta, Peter}, title = {Die Tschechoslowakai seit 1918 : der rechtliche Status der Juden nach der Verfassung und dem Sprachengesetz von 1920}, series = {Handbuch zur Geschichte der Juden in Europa}, journal = {Handbuch zur Geschichte der Juden in Europa}, publisher = {Primus Verlag}, address = {Frankfurt a.M.}, isbn = {978-3-86312-363-5}, pages = {143 -- 150}, year = {2014}, language = {de} } @article{FranzJilge2014, author = {Franz, Norbert P. and Jilge, Wilfried}, title = {Rußland, Ukraine, Weißrußland, Baltikum (Lettland, Estland)}, series = {Handbuch zur Geschichte der Juden in Europa}, journal = {Handbuch zur Geschichte der Juden in Europa}, publisher = {Primus Verlag}, address = {Frankfurt a.M.}, isbn = {978-3-86312-363-5}, pages = {167 -- 227}, year = {2014}, language = {de} } @article{Reso2014, author = {Reso, Judith}, title = {Protein Classification Workflow}, series = {Process Design for Natural Scientists: an agile model-driven approach}, journal = {Process Design for Natural Scientists: an agile model-driven approach}, number = {500}, editor = {Lambrecht, Anna-Lena and Margaria, Tiziana}, publisher = {Springer Verlag}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {65 -- 72}, year = {2014}, abstract = {The protein classification workflow described in this report enables users to get information about a novel protein sequence automatically. The information is derived by different bioinformatic analysis tools which calculate or predict features of a protein sequence. Also, databases are used to compare the novel sequence with known proteins.}, language = {en} } @article{Schulze2014, author = {Schulze, Gunnar}, title = {Workflow for rapid metagenome analysis}, series = {Process design for natural scientists: an agile model-driven approach}, journal = {Process design for natural scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {88 -- 100}, year = {2014}, abstract = {Analyses of metagenomes in life sciences present new opportunities as well as challenges to the scientific community and call for advanced computational methods and workflows. The large amount of data collected from samples via next-generation sequencing (NGS) technologies render manual approaches to sequence comparison and annotation unsuitable. Rather, fast and efficient computational pipelines are needed to provide comprehensive statistics and summaries and enable the researcher to choose appropriate tools for more specific analyses. The workflow presented here builds upon previous pipelines designed for automated clustering and annotation of raw sequence reads obtained from next-generation sequencing technologies such as 454 and Illumina. Employing specialized algorithms, the sequence reads are processed at three different levels. First, raw reads are clustered at high similarity cutoff to yield clusters which can be exported as multifasta files for further analyses. Independently, open reading frames (ORFs) are predicted from raw reads and clustered at two strictness levels to yield sets of non-redundant sequences and ORF families. Furthermore, single ORFs are annotated by performing searches against the Pfam database}, language = {en} } @article{Vierheller2014, author = {Vierheller, Janine}, title = {Exploratory Data Analysis}, series = {Process Design for Natural Scientists: an agile model-driven approach}, journal = {Process Design for Natural Scientists: an agile model-driven approach}, number = {500}, editor = {Lambrecht, Anna-Lena and Margaria, Tiziana}, publisher = {Axel Springer Verlag}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {110 -- 126}, year = {2014}, abstract = {In bioinformatics the term exploratory data analysis refers to different methods to get an overview of large biological data sets. Hence, it helps to create a framework for further analysis and hypothesis testing. The workflow facilitates this first important step of the data analysis created by high-throughput technologies. The results are different plots showing the structure of the measurements. The goal of the workflow is the automatization of the exploratory data analysis, but also the flexibility should be guaranteed. The basic tool is the free software R.}, language = {en} } @article{Schuett2014, author = {Sch{\"u}tt, Christine}, title = {Identification of differentially expressed genes}, series = {Process design for natural scientists: an agile model-driven approach}, journal = {Process design for natural scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {127 -- 139}, year = {2014}, abstract = {With the jABC it is possible to realize workflows for numerous questions in different fields. The goal of this project was to create a workflow for the identification of differentially expressed genes. This is of special interest in biology, for it gives the opportunity to get a better insight in cellular changes due to exogenous stress, diseases and so on. With the knowledge that can be derived from the differentially expressed genes in diseased tissues, it becomes possible to find new targets for treatment.}, language = {en} } @article{Kuntzsch2014, author = {Kuntzsch, Christian}, title = {Visualization of data transfer paths}, series = {Process design for natural scientists: an agile model-driven approach}, journal = {Process design for natural scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {140 -- 148}, year = {2014}, abstract = {A workflow for visualizing server connections using the Google Maps API was built in the jABC. It makes use of three basic services: An XML-based IP address geolocation web service, a command line tool and the Static Maps API. The result of the workflow is an URL leading to an image file of a map, showing server connections between a client and a target host.}, language = {en} } @article{Hibbe2014, author = {Hibbe, Marcel}, title = {Spotlocator - Guess Where the Photo Was Taken!}, series = {Process Design for Natural Scientists: an agile model-driven approach}, journal = {Process Design for Natural Scientists: an agile model-driven approach}, number = {500}, editor = {Lambrecht, Anna-Lena and Margaria, Tiziana}, publisher = {Springer Verlag}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {149 -- 160}, year = {2014}, abstract = {Spotlocator is a game wherein people have to guess the spots of where photos were taken. The photos of a defined area for each game are from panoramio.com. They are published at http://spotlocator. drupalgardens.com with an ID. Everyone can guess the photo spots by sending a special tweet via Twitter that contains the hashtag \#spotlocator, the guessed coordinates and the ID of the photo. An evaluation is published for all tweets. The players are informed about the distance to the real photo spots and the positions are shown on a map.}, language = {en} } @article{Blaese2014, author = {Blaese, Leif}, title = {Data mining for unidentified protein squences}, series = {Process design for natural scientists: an agile model-driven approach}, journal = {Process design for natural scientists: an agile model-driven approach}, number = {500}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {73 -- 87}, year = {2014}, abstract = {Through the use of next generation sequencing (NGS) technology, a lot of newly sequenced organisms are now available. Annotating those genes is one of the most challenging tasks in sequence biology. Here, we present an automated workflow to find homologue proteins, annotate sequences according to function and create a three-dimensional model.}, language = {en} } @article{Lis2014, author = {Lis, Monika}, title = {Constructing a Phylogenetic Tree}, series = {Process Design for Natural Scientists: an agile model-driven approach}, journal = {Process Design for Natural Scientists: an agile model-driven approach}, number = {500}, editor = {Lambrecht, Anna-Lena and Margaria, Tiziana}, publisher = {Springer Verlag}, address = {Berlin}, isbn = {978-3-662-45005-5}, issn = {1865-0929}, pages = {101 -- 109}, year = {2014}, abstract = {In this project I constructed a workflow that takes a DNA sequence as input and provides a phylogenetic tree, consisting of the input sequence and other sequences which were found during a database search. In this phylogenetic tree the sequences are arranged depending on similarities. In bioinformatics, constructing phylogenetic trees is often used to explore the evolutionary relationships of genes or organisms and to understand the mechanisms of evolution itself.}, language = {en} } @article{Stillmark2014, author = {Stillmark, Hans-Christian}, title = {Alexander Stillmarks Wechsel von L{\"u}beck nach Erfurt - 1949 ein ungew{\"o}hnliches Engagement}, series = {Reizland DDR. Deutungen und Selbstdeutungen literarischer West-Ost-Migration}, journal = {Reizland DDR. Deutungen und Selbstdeutungen literarischer West-Ost-Migration}, editor = {Bircken, Margrid and Degen, Andreas}, publisher = {Vandenhoeck \& Ruprecht}, address = {G{\"o}ttingen}, isbn = {978-3-847002-55-0}, pages = {87 -- 102}, year = {2014}, abstract = {Die Migration von etwa einer halben Million Menschen aus westlichen L{\"a}ndern in die DDR ist wenig erforscht. Dies gilt auch f{\"u}r das Gebiet der Literatur. Was so unterschiedliche Autoren wie Anna Seghers, Arnolt Bronnen, Heinar Kipphardt, Adolf Endler, Wolf Biermann, Gisela Kraft oder Ronald M. Schernikau verbindet, ist, sich einmal f{\"u}r ein Leben in der DDR entschieden zu haben. Einige von ihnen fanden in der sozialistischen Wahlheimat ihre Themen und ihr Publikum: h{\"a}ufig in zunehmender Distanz zur Kulturpolitik, mitunter auch in großer N{\"a}he. Andere kehrten dem Land nach wenigen Jahren entt{\"a}uscht oder verbittert wieder den R{\"u}cken oder wurden hinausgedr{\"a}ngt. Die Beitr{\"a}ge besch{\"a}ftigen sich mit den Motiven, Umst{\"a}nden, biographischen Konsequenzen, Selbstdeutungen und literarischen Resultaten der {\"U}bersiedlung in die SBZ bzw. in die DDR zwischen 1945 und 1989.}, language = {de} } @article{Stillmark2014, author = {Stillmark, Hans-Christian}, title = {„Sieh diese Flasche Branntwein in dieser Faust"- Wolfgang Hilbig}, series = {Realit{\"a}tsflucht und Erkenntnissucht. Alkohol und Literatur}, journal = {Realit{\"a}tsflucht und Erkenntnissucht. Alkohol und Literatur}, editor = {Bernauer, Markus and Gemmel, Mirko}, publisher = {Ripperger \& Kremers}, address = {Berlin}, isbn = {978-3-943999-08-2}, pages = {237 -- 258}, year = {2014}, abstract = {Schriftsteller schreiben nicht weil, sondern obwohl sie trinken. Der Trinker als literarische Figur und der Schriftsteller als Trinker, zwischen diesen beiden Punkten oszilliert das Thema »Alkohol und Literatur«. In typischer Alkoholikermanier bewegen sich die Texte der betreffenden Autoren zwischen alkoholseliger {\"U}bertreibung und erstaunlich zarter Empfindsamkeit und sind zugleich Zeichen f{\"u}r eine Flucht vor der tief empfundenen Unmenschlichkeit der modernen Welt sowie dem eigenen Versagensgef{\"u}hl. Die oft satirischen und zumeist hoch intelligenten Reflexionen der trinkenden Helden geraten in den literarischen Texten zu aberwitzigen Monologen, in denen alle moralischen und geistigen Werte demontiert werden und die Realit{\"a}t nicht nur im Chaos versinkt, sondern gar deren Existenz {\"u}berhaupt in Frage gestellt wird.}, language = {de} } @article{Heimann2014, author = {Heimann, Heinz-Dieter}, title = {Wir alle sind Nachbarn?}, series = {T{\"u}r an T{\"u}r und Haus an Haus Nachbarschaften in der historischen Stadt Themenbuch 2014}, journal = {T{\"u}r an T{\"u}r und Haus an Haus Nachbarschaften in der historischen Stadt Themenbuch 2014}, publisher = {Arbeitsgemeinschaft St{\"a}dte mit historischen Stadkernen des Landes Brandenburg}, address = {Potsdam}, isbn = {978-3-00-047888-8}, pages = {18 -- 23}, year = {2014}, language = {de} } @article{Buetow2014, author = {B{\"u}tow, Sascha}, title = {Vielheit statt Einheit}, series = {T{\"u}r an T{\"u}r und Haus an Haus Nachbarschaften in der historischen Stadt Themenbuch 2014}, journal = {T{\"u}r an T{\"u}r und Haus an Haus Nachbarschaften in der historischen Stadt Themenbuch 2014}, publisher = {Arbeitsgemeinschaft St{\"a}dte mit historischen Stadkernen des Landes Brandenburg}, address = {Potsdam}, isbn = {978-3-00-047888-8}, pages = {54 -- 57}, year = {2014}, language = {de} } @article{WeberNimmich2014, author = {Weber, Edzard and Nimmich, Andre}, title = {Meta-Kennzahlen f{\"u}r die Bewertung von Dienstleistungen}, series = {Handbuch prozessorientiertes Wissensmanagment}, journal = {Handbuch prozessorientiertes Wissensmanagment}, publisher = {GITO}, address = {Berlin}, isbn = {978-3-95545-026-7}, pages = {95 -- 115}, year = {2014}, language = {de} } @article{Eggert2014, author = {Eggert, Sandy}, title = {Gestaltung wandlungsf{\"a}higer Unternehmensprozesse}, series = {Handbuch prozessorientiertes Wissensmanagment}, journal = {Handbuch prozessorientiertes Wissensmanagment}, publisher = {GITO}, address = {Berlin}, isbn = {978-3-95545-026-7}, pages = {123 -- 129}, year = {2014}, language = {de} } @article{LembckeWeber2014, author = {Lembcke, Robert and Weber, Edzard}, title = {Methode zur Modellierung und Analyse der Kommunikationsstrukturen}, series = {Handbuch prozessorientiertes Wissensmanagement}, journal = {Handbuch prozessorientiertes Wissensmanagement}, publisher = {GITO}, address = {Berlin}, isbn = {978-3-95545-026-7}, pages = {239 -- 263}, year = {2014}, language = {de} } @article{WeberLembcke2014, author = {Weber, Edzard and Lembcke, Robert}, title = {Fallstudien: Analyse der Kommunikation}, series = {Handbuch prozessorientiertes Wissensmanagment}, journal = {Handbuch prozessorientiertes Wissensmanagment}, publisher = {GITO}, address = {Berlin}, isbn = {978-3-95545-026-7}, pages = {267 -- 294}, year = {2014}, language = {de} } @article{MuellerKruegerZeier2014, author = {M{\"u}ller, J{\"u}rgen and Kr{\"u}ger, Jens and Zeier, Alexander}, title = {Unternehmensportale als Quelle f{\"u}r relevante Informationen}, series = {Handbuch prozessorientierters Wissensmanagment}, journal = {Handbuch prozessorientierters Wissensmanagment}, publisher = {GITO}, address = {Berlin}, isbn = {978-3-95545-026-7}, pages = {299 -- 307}, year = {2014}, language = {de} } @article{WeberScharff2014, author = {Weber, Edzard and Scharff, Christian}, title = {Gesch{\"a}ftsprozessanalyse und Wissensmanagement mit Hilfe Sozialer Netzwerke}, series = {Handbuch prozessorientiertes Wissensmanagement}, journal = {Handbuch prozessorientiertes Wissensmanagement}, publisher = {GITO}, address = {Berlin}, isbn = {978-3-95545-026-7}, pages = {311 -- 320}, year = {2014}, language = {de} } @article{DorschDoerfler2014, author = {Dorsch, Christian and D{\"o}rfler, Thomas}, title = {Organized hypocrisy of the international community}, series = {Zeitschrift f{\"u}r Genozidforschung}, volume = {15}, journal = {Zeitschrift f{\"u}r Genozidforschung}, number = {1-2}, publisher = {Velbr{\"u}ck Wissenschaft}, address = {Weilerswist}, issn = {1438-8332}, doi = {10.5771/1438-8332-2014-1-2-8}, pages = {8 -- 31}, year = {2014}, language = {en} } @article{RichterDoellner2014, author = {Richter, Rico and D{\"o}llner, J{\"u}rgen Roland Friedrich}, title = {Concepts and techniques for integration, analysis and visualization of massive 3D point clouds}, series = {Computers, environment and urban systems}, volume = {45}, journal = {Computers, environment and urban systems}, publisher = {Elsevier}, address = {Oxford}, issn = {0198-9715}, doi = {10.1016/j.compenvurbsys.2013.07.004}, pages = {114 -- 124}, year = {2014}, abstract = {Remote sensing methods, such as LiDAR and image-based photogrammetry, are established approaches for capturing the physical world. Professional and low-cost scanning devices are capable of generating dense 3D point clouds. Typically, these 3D point clouds are preprocessed by GIS and are then used as input data in a variety of applications such as urban planning, environmental monitoring, disaster management, and simulation. The availability of area-wide 3D point clouds will drastically increase in the future due to the availability of novel capturing methods (e.g., driver assistance systems) and low-cost scanning devices. Applications, systems, and workflows will therefore face large collections of redundant, up-to-date 3D point clouds and have to cope with massive amounts of data. Hence, approaches are required that will efficiently integrate, update, manage, analyze, and visualize 3D point clouds. In this paper, we define requirements for a system infrastructure that enables the integration of 3D point clouds from heterogeneous capturing devices and different timestamps. Change detection and update strategies for 3D point clouds are presented that reduce storage requirements and offer new insights for analysis purposes. We also present an approach that attributes 3D point clouds with semantic information (e.g., object class category information), which enables more effective data processing, analysis, and visualization. Out-of-core real-time rendering techniques then allow for an interactive exploration of the entire 3D point cloud and the corresponding analysis results. Web-based visualization services are utilized to make 3D point clouds available to a large community. The proposed concepts and techniques are designed to establish 3D point clouds as base datasets, as well as rendering primitives for analysis and visualization tasks, which allow operations to be performed directly on the point data. Finally, we evaluate the presented system, report on its applications, and discuss further research challenges.}, language = {en} } @article{OmosaAmuguneNdundaetal.2014, author = {Omosa, Leonidah K. and Amugune, Beatrice and Ndunda, Beth and Milugo, Trizah K. and Heydenreich, Matthias and Yenesew, Abiy and Midiwo, Jacob O.}, title = {Antimicrobial flavonoids and diterpenoids from Dodonaea angustifolia}, series = {South African journal of botany : an international interdisciplinary journal for botanical sciences}, volume = {91}, journal = {South African journal of botany : an international interdisciplinary journal for botanical sciences}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0254-6299}, doi = {10.1016/j.sajb.2013.11.012}, pages = {58 -- 62}, year = {2014}, language = {en} } @article{LamannaKirschbaumTiedemann2014, author = {Lamanna, Francesco and Kirschbaum, Frank and Tiedemann, Ralph}, title = {De novo assembly and characterization of the skeletal muscle and electric organ transcriptomes of the African weakly electric fish Campylomormyrus compressirostris (Mormyridae, Teleostei)}, series = {Molecular ecology resources}, volume = {14}, journal = {Molecular ecology resources}, number = {6}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {1755-098X}, doi = {10.1111/1755-0998.12260}, pages = {1222 -- 1230}, year = {2014}, abstract = {African weakly electric fishes (Mormyridae) underwent an outstanding adaptive radiation (about 200 species), putatively owing to their ability to communicate through species-specific weak electric signals. The electric organ discharge (EOD) is produced by muscle-derived electrocytes organized in piles to form an electric organ. Despite the importance of this trait as a prezygotic isolation mechanism, genomic resources remained limited. We present here a first draft of the skeletal muscle and electric organ transcriptomes from the weakly electric fish species Campylomormyrus compressirostris, obtained using the Illumina HiSeq2000 sequencing technology. Approximately 6.8 Gbp of cDNA sequence data were produced from both tissues, resulting in 57268109 raw reads for the skeletal muscle and 46934923 for the electric organ, and assembled de novo into 46143 and 89270 contigs, respectively. About 50\% of both transcriptomes were annotated after protein databases search. The two transcriptomes show similar profiles in terms of Gene Ontology categories composition. We identified several candidate genes which are likely to play a central role in the production and evolution of the electric signal. For most of these genes, and for many other housekeeping genes, we were able to obtain the complete or partial coding DNA sequences (CDS), which can be used for the development of primers to be utilized in qRT-PCR experiments. We present also the complete mitochondrial genome and compare it to those available from other weakly electric fish species. Additionally, we located 1671 SSR-containing regions with their flanking sites and designed the relative primers. This study establishes a first step in the development of genomic tools aimed at understanding the role of electric communication during speciation.}, language = {en} } @article{StiefAltmannHoffmannetal.2014, author = {Stief, Anna and Altmann, Simone and Hoffmann, Karen and Pant, Bikram Datt and Scheible, Wolf-R{\"u}diger and B{\"a}urle, Isabel}, title = {Arabidopsis miR156 regulates tolerance to recurring environmental stress through SPL transcription factors}, series = {The plant cell}, volume = {26}, journal = {The plant cell}, number = {4}, publisher = {American Society of Plant Physiologists}, address = {Rockville}, issn = {1040-4651}, doi = {10.1105/tpc.114.123851}, pages = {1792 -- 1807}, year = {2014}, abstract = {Plants are sessile organisms that gauge stressful conditions to ensure survival and reproductive success. While plants in nature often encounter chronic or recurring stressful conditions, the strategies to cope with those are poorly understood. Here, we demonstrate the involvement of ARGONAUTE1 and the microRNA pathway in the adaptation to recurring heat stress (HS memory) at the physiological and molecular level. We show that miR156 isoforms are highly induced after HS and are functionally important for HS memory. miR156 promotes sustained expression of HS-responsive genes and is critical only after HS, demonstrating that the effects of modulating miR156 on HS memory do not reflect preexisting developmental alterations. miR156 targets SPL transcription factor genes that are master regulators of developmental transitions. SPL genes are posttranscriptionally downregulated by miR156 after HS, and this is critical for HS memory. Altogether, the miR156-SPL module mediates the response to recurring HS in Arabidopsis thaliana and thus may serve to integrate stress responses with development.}, language = {en} } @article{KabelitzKappelHennebergeretal.2014, author = {Kabelitz, Tina and Kappel, Christian and Henneberger, Kirstin and Benke, Eileen and Noeh, Christiane and B{\"a}urle, Isabel}, title = {eQTL mapping of transposon silencing reveals a position-dependent stable escape from epigenetic silencing and transposition of AtMu1 in thee arabidopsis lineage}, series = {The plant cell}, volume = {26}, journal = {The plant cell}, number = {8}, publisher = {American Society of Plant Physiologists}, address = {Rockville}, issn = {1040-4651}, doi = {10.1105/tpc.114.128512}, pages = {3261 -- 3271}, year = {2014}, abstract = {Transposons are massively abundant in all eukaryotic genomes and are suppressed by epigenetic silencing. Transposon activity contributes to the evolution of species; however, it is unclear how much transposition-induced variation exists at a smaller scale and how transposons are targeted for silencing. Here, we exploited differential silencing of the AtMu1c transposon in the Arabidopsis thaliana accessions Columbia (Col) and Landsberg erecta (Ler). The difference persisted in hybrids and recombinant inbred lines and was mapped to a single expression quantitative trait locus within a 20-kb interval. In Ler only, this interval contained a previously unidentified copy of AtMu1c, which was inserted at the 39 end of a protein-coding gene and showed features of expressed genes. By contrast, AtMu1c(Col) was intergenic and associated with heterochromatic features. Furthermore, we identified widespread natural AtMu1c transposition from the analysis of over 200 accessions, which was not evident from alignments to the reference genome. AtMu1c expression was highest for insertions within 39 untranslated regions, suggesting that this location provides protection from silencing. Taken together, our results provide a species-wide view of the activity of one transposable element at unprecedented resolution, showing that AtMu1c transposed in the Arabidopsis lineage and that transposons can escape epigenetic silencing by inserting into specific genomic locations, such as the 3' end of genes.}, language = {en} } @article{D'Aprile2014, author = {D'Aprile, Iwan-Michelangelo}, title = {Aufkl{\"a}rungstransfer zwischen Sachsen und Preußen}, isbn = {978-3-95498-084-0}, year = {2014}, language = {de} } @article{D'Aprile2014, author = {D'Aprile, Iwan-Michelangelo}, title = {Mediums of communication and the development of an European constitutional discourse at the dawn of the nineteenth century}, series = {Ayer : revista de historia contempor{\´a}nea}, journal = {Ayer : revista de historia contempor{\´a}nea}, number = {94}, publisher = {Asociaci{\´o}n de Historia Contempor{\´a}nea}, address = {Madrid}, issn = {1134-2277}, pages = {49 -- 69}, year = {2014}, abstract = {In this article, entangled media history is presented as an approach to combine recent methodological developments towards an international turn in intellectual history on the one hand with the social history of ideas on the other. By concentrating on press networks, publishers, and media formats the various processes of constitutionalization and nationalization in the aftermath of the French Revolution and the Napoleonic period can be reconstructed as part of an emerging European public sphere. This is exemplified by the example of the political discourse of that time in Germany. In a first step, the international networks around the two most important German publishers, Johann Friedrich Cotta and Friedrich Arnold Brockhaus, are reconstructed. In the second part, the crucial role of translations and adaptations of political articles in two decisive media formats of the political discourse, the historical-political journals and the "Conversationslexikon" is examined. And finally in a third step, the specific significance of the reception of the Spanish revolution of the "trienio liberal" for the development of a constitutional vocabulary in Germany is sketched.}, language = {es} } @article{KoesslHechavarriaVossetal.2014, author = {Koessl, M. and Hechavarria, J. C. and Voss, C. and Macias, S. and Mora, E. C. and Vater, Marianne}, title = {Neural maps for target range in the auditory cortex of echolocating bats}, series = {Current opinion in neurobiology : reviews of all advances ; evaluation of key references ; comprehensive listing of papers}, volume = {24}, journal = {Current opinion in neurobiology : reviews of all advances ; evaluation of key references ; comprehensive listing of papers}, publisher = {Elsevier}, address = {London}, issn = {0959-4388}, doi = {10.1016/j.conb.2013.08.016}, pages = {68 -- 75}, year = {2014}, abstract = {Computational brain maps as opposed to maps of receptor surfaces strongly reflect functional neuronal design principles. In echolocating bats, computational maps are established that topographically represent the distance of objects. These target range maps are derived from the temporal delay between emitted call and returning echo and constitute a regular representation of time (chronotopy). Basic features of these maps are innate, and in different bat species the map size and precision varies. An inherent advantage of target range maps is the implementation of mechanisms for lateral inhibition and excitatory feedback. Both can help to focus target ranging depending on the actual echolocation situation. However, these maps are not absolutely necessary for bat echolocation since there are bat species without cortical target-distance maps, which use alternative ensemble computation mechanisms.}, language = {en} } @article{AustinGroppeElsner2014, author = {Austin, Gina and Groppe, Karoline and Elsner, Birgit}, title = {The reciprocal relationship between executive function and theory of mind in middle childhood: a 1-year longitudinal perspective}, series = {Frontiers in psychology}, volume = {5}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2014.00655}, pages = {11}, year = {2014}, abstract = {There is robust evidence showing a link between executive function (EF) and theory of mind (ToM) in 3-to 5-year-olds. However, it is unclear whether this relationship extends to middle childhood. In addition, there has been much discussion about the nature of this relationship. Whereas some authors claim that ToM is needed for EF, others argue that ToM requires EF. To date, however, studies examining the longitudinal relationship between distinct sub components of EF [i.e., attention shifting, working memory (WM) updating, inhibition] and ToM in middle childhood are rare. The present study examined (1) the relationship between three EF subcomponents (attention shifting, WM updating, inhibition) and ToM in middle childhood, and (2) the longitudinal reciprocal relationships between the EF subcomponents and ToM across a 1-year period. EF and ToM measures were assessed experimentally in a sample of 1,657 children (aged 6-11 years) at time point one (t1) and 1 year later at time point two (t2). Results showed that the concurrent relationships between all three EF subcomponents and ToM pertained in middle childhood at t1 and t2, respectively, even when age, gender, and fluid intelligence were partialle dout. Moreover, cross-lagged structural equation modeling (again, controlling for age, gender, and fluid intelligence, as well as for the earlier levels of the target variables), revealed partial support for the view that early ToM predictslater EF, but stronger evidence for the assumption that early EF predictslater ToM. The latter was found for attention shifting and WM updating, but not for inhibition. This reveals the importance of studying the exact interplay of ToM and EF across childhood development, especially with regard to different EF subcomponents. Most likely, understanding others' mental states at different levels of perspective-taking requires specific EF subcomponents, suggesting developmental change in the relations between EF and ToM across childhood.}, language = {en} } @article{PrimusRitschelSigueenzaetal.2014, author = {Primus, Philipp-Alexander and Ritschel, Thomas and Sigueenza, Pilar Y. and Cauqui, Miguel Angel and Hernandez-Garrido, Juan Carlos and Kumke, Michael Uwe}, title = {High-resolution spectroscopy of europium-doped ceria as a tool to correlate structure and catalytic activity}, series = {The journal of physical chemistry : C, Nanomaterials and interfaces}, volume = {118}, journal = {The journal of physical chemistry : C, Nanomaterials and interfaces}, number = {40}, publisher = {American Chemical Society}, address = {Washington}, issn = {1932-7447}, doi = {10.1021/jp505467r}, pages = {23349 -- 23360}, year = {2014}, abstract = {Site-selective emission spectra of Eu3+-doped CeO2 nanoparticles up to the D-5(0) - F-7(5) transition were recorded under cryogenic conditions to identify the local structure around the Eu3+ dopants in ceria. It is found that pretreatment conditions are crucial for the redistribution of dopants from a broad variety of environments to six well-defined lattice sites. The influence of the dopant and the host structure on the catalytic activity was investigated. A relationship between structure and reactivity is discussed. It is shown that oxygen transport is most efficient in particles with a pronounced amorphous character.}, language = {en} } @article{DevosGraefField2014, author = {Devos, Damien P. and Gr{\"a}f, Ralph and Field, Mark C.}, title = {Evolution of the nucleus}, series = {Current opinion in cell biology : review articles, recommended reading, bibliography of the world literature}, volume = {28}, journal = {Current opinion in cell biology : review articles, recommended reading, bibliography of the world literature}, publisher = {Elsevier}, address = {London}, issn = {0955-0674}, doi = {10.1016/j.ceb.2014.01.004}, pages = {8 -- 15}, year = {2014}, abstract = {The nucleus represents a major evolutionary transition. As a consequence of separating translation from transcription many new functions arose, which likely contributed to the remarkable success of eukaryotic cells. Here we will consider what has recently emerged on the evolutionary histories of several key aspects of nuclear biology; the nuclear pore complex, the lamina, centrosomes and evidence for prokaryotic origins of relevant players.}, language = {en} } @article{Ifenthaler2014, author = {Ifenthaler, Dirk}, title = {Challenges of a Holistic Learning Analytics Project}, series = {E-Learning Symposium 2014 : Mobil und vernetzt - studieren im digitalen Zeitalter ; Potsdam, 14. November 2014}, journal = {E-Learning Symposium 2014 : Mobil und vernetzt - studieren im digitalen Zeitalter ; Potsdam, 14. November 2014}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, doi = {10.25932/publishup-44230}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-442303}, pages = {15 -- 28}, year = {2014}, abstract = {Recently, interest in collecting and mining large sets of educational data on student background and performance to conduct research on learning and instruction has developed as an area generally referred to as learning analytics. Higher education leaders are recognising the value of learning analytics for improving not only learning and teaching but also the entire educational arena. However, theoretical concepts and empirical evidence need to be generated within the fast evolving field of learning analytics. In this paper, we introduce a holistic learning analytics framework. Based on this framework, student, learning, and curriculum profiles have been developed which include relevant static and dynamic parameters for facilitating the learning analytics framework. Based on the theoretical model, an empirical study was conducted to empirically validate the parameters included in the student profile. The paper concludes with practical implications and issues for future research.}, language = {en} } @article{ZoernerKoehlmannBrandt2014, author = {Zoerner, Dietmar and K{\"o}hlmann, Wiebke and Brandt, Christopher}, title = {Mobiles spielebasiertes Lernen an historischen Lernorten}, series = {E-Learning Symposium 2014 : Mobil und vernetzt - studieren im digitalen Zeitalter ; Potsdam, 14. November 2014}, journal = {E-Learning Symposium 2014 : Mobil und vernetzt - studieren im digitalen Zeitalter ; Potsdam, 14. November 2014}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, doi = {10.25932/publishup-44235}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-442354}, pages = {53 -- 54}, year = {2014}, abstract = {Im Rahmen eines interdisziplin{\"a}ren studentischen Projekts wurde ein Framework f{\"u}r mobile pervasive Lernspiele entwickelt. Am Beispiel des historischen Lernortes Park Sanssouci wurde auf dieser Grundlage ein Lernspiel f{\"u}r Sch{\"u}lerinnen und Sch{\"u}ler implementiert. Die geplante Evaluation soll die Lernwirksamkeit von geobasierten mobilen Lernspielen messen. Dazu wird die Intensit{\"a}t des Flow-Erlebens mit einer ortsgebundenen alternativen Umsetzung verglichen.}, language = {de} } @article{PutraNeuberReichetzederetal.2014, author = {Putra, Sulistyo Emantoko Dwi and Neuber, Corinna and Reichetzeder, Christoph and Hocher, Berthold and Kleuser, Burkhard}, title = {Analysis of genomic DNA methylation levels in human placenta using liquid Chromatography-Electrospray ionization tandem mass spectrometry}, series = {Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry and pharmacology}, volume = {33}, journal = {Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry and pharmacology}, number = {4}, publisher = {Karger}, address = {Basel}, issn = {1015-8987}, doi = {10.1159/000358666}, pages = {945 -- 952}, year = {2014}, abstract = {Background: DNA-methylation is a common epigenetic tool which plays a crucial role in gene regulation and is essential for cell differentiation and embryonic development. The placenta is an important organ where gene activity can be regulated by epigenetic DNA modifications, including DNA methylation. This is of interest as, the placenta is the interface between the fetus and its environment, the mother. Exposure to environmental toxins and nutrition during pregnancy may alter DNA methylation of the placenta and subsequently placental function and as a result the phenotype of the offspring. The aim of this study was to develop a reliable method to quantify DNA methylation in large clinical studies. This will be a tool to analyze the degree of DNA methylation in the human placenta in relationship to clinical readouts. Methods: Liquid chromatography-electrospray ionization/multi-stage mass spectrometry (LC-ESI/MS/MS) technique was used for the quantification of the 5dmC/dG ratio in placentas from 248 healthy pregnancies. We were able to demonstrate that this method is a reliable and stable way to determine global placental DNA methylation in large clinical trials. Results/Conclusion: The degree of placental DNA methylation seen in our pilot study varies substantially from 2\% to 5\%. The clinical implications of this variation need to be demonstrated in adequately powered large studies.}, language = {en} } @article{Risse2014, author = {Risse, Sarah}, title = {Effects of visual span on reading speed and parafoveal processing in eye movements during sentence reading}, series = {Journal of vision}, volume = {14}, journal = {Journal of vision}, number = {8}, publisher = {Association for Research in Vision and Opthalmology}, address = {Rockville}, issn = {1534-7362}, doi = {10.1167/14.8.11}, pages = {13}, year = {2014}, language = {en} } @article{SchulzeAppelhansTierschetal.2014, author = {Schulze, Nicole and Appelhans, D. and Tiersch, Brigitte and Koetz, Joachim}, title = {Morphological transformation of vesicles into tubular structures by adding polyampholytes or dendritic glycopolymers}, series = {Colloids and surfaces : an international journal devoted to the principles and applications of colloid and interface science ; A, Physicochemical and engineering aspects}, volume = {457}, journal = {Colloids and surfaces : an international journal devoted to the principles and applications of colloid and interface science ; A, Physicochemical and engineering aspects}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0927-7757}, doi = {10.1016/j.colsurfa.2014.06.007}, pages = {326 -- 332}, year = {2014}, abstract = {For the first time tubulating properties of spherical dendritic glycopolymers and linear alternating polyampholytes against non-uniform negatively charged giant vesicles are proven by light microscopy and cryo-scanning electron microscopy study. Real time observation of the morphological transformation from giant vesicles to tubular structures, simulating morphogenesis in living cells, is given by using the cationic and H-bond active dendritic glycopolymer accompanied by reducing the size of the giant vesicles and the evidence of vesicle-vesicle interaction which was only postulated in a previous study. Similar morphogenesis of non-uniform giant vesicles into tubular network structure can be observed by using a polyampholyte in the stretched conformation at pH 9. Pearl necklace and tubular network structure formation are also observed by applying anionic vesicles of significant smaller dimensions with average size dimensions of 35 nm, after adding the polyampholyte at pH 9. However, the fitting accuracy between the functional groups along the backbone chain of the polyampholyte on one side and the vesicle surface on the other side is of high importance for the transformation process by using polyampholytes. The resulting tubular and network structures offer new fields of application as microfluidic transport channels or template phases for the shape controlled formation of nanoparticles. (C) 2014 Elsevier B.V. All rights reserved.}, language = {en} } @article{GroppeElsner2014, author = {Groppe, Karoline and Elsner, Birgit}, title = {Executive function and food approach behavior in middle childhood}, series = {Frontiers in psychology}, volume = {5}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2014.00447}, pages = {12}, year = {2014}, abstract = {Executive function (EF) has long been considered to be a unitary, domain-general cognitive ability. However, recent research suggests differentiating "hot" affective and "cool" cognitive aspects of EF. Yet, findings regarding this two-factor construct are still inconsistent. In particular, the development of this factor structure remains unclear and data on school-aged children is lacking. Furthermore, studies linking EF and overweight or obesity suggest that EF contributes to the regulation of eating behavior. So far, however, the links between EF and eating behavior have rarely been investigated in children and non-clinical populations. First, we examined whether EF can be divided into hot and cool factors or whether they actually correspond to a unitary construct in middle childhood. Second, we examined how hot and cool EF are associated with different eating styles that put children at risk of becoming overweight during development. Hot and cool EF were assessed experimentally in a non-clinical population of 1657 elementary-school children (aged 6-11 years). The "food approach" behavior was rated mainly via parent questionnaires. Findings indicate that hot EF is distinguishable from cool EF. However, only cool EF seems to represent a coherent functional entity, whereas hot EF does not seem to be a homogenous construct. This was true for a younger and an older subgroup of children. Furthermore, different EF components were correlated with eating styles, such as responsiveness to food, desire to drink, and restrained eating in girls but not in boys. This shows that lower levels of EF are not only seen in clinical populations of obese patients but are already associated with food approach styles in a normal population of elementary school-aged girls. Although the direction of effect still has to be clarified, results point to the possibility that EF constitutes a risk factor for eating styles contributing to the development of overweight in the long-term.}, language = {en} } @article{RofesBastiaanseMartinezFerreiro2014, author = {Rofes, Adria and Bastiaanse, Roelien and Martinez-Ferreiro, Silvia}, title = {Conditional and future tense impairment in non-fluent aphasia}, series = {Aphasiology : an international, interdisciplinary journal}, volume = {28}, journal = {Aphasiology : an international, interdisciplinary journal}, number = {1}, publisher = {Routledge, Taylor \& Francis Group}, address = {Abingdon}, issn = {0268-7038}, doi = {10.1080/02687038.2013.850650}, pages = {99 -- 115}, year = {2014}, abstract = {Background: Morphological errors of tense and agreement are salient in agrammatic aphasia. The PADILIH predicts impairments in discourse linking that translate to greater difficulties in referring to a past event time than to a future or a present event time. In Catalan, the Periphrastic conditional tense (e.g., if the man had had time, he would have...) refers to the past and the Simple conditional tense refers to the future (e.g., if the man had time, he would...). These two tenses refer to an event that may happen (irrealis).Aims: We fill in the gap of the conditional tense and provide further data to study contrasts in verb inflection for time reference. We predict that verb forms that refer to an irrealis past event (Periphrastic conditional) are more impaired than forms that refer to an irrealis future event (Simple conditional and Future). We also predict that there are no differences between verb forms that refer to an irrealis future event (Simple conditional and Future). We also assessed whether problems in time reference extend to individuals with non-fluent aphasia that are not typical agrammatic Broca aphasia.Methods \& Procedures: A sentence completion task that included 60 sentences (20 per type) of equal length in a Conditional structure (if-sentences) was designed. We tested three sentence types: Periphrastic conditional, Simple conditional and Future. The task was administered to nine participants with non-fluent aphasia and nine age-matched non-brain-damaged participants.Outcomes \& Results: The Control group scored at ceiling on the three sentence types. Participants with non-fluent aphasia were most impaired in the production of the Periphrastic conditional as compared with the Simple conditional and the Future.Conclusions: When irrealis event times are compared, past events are more impaired than future events. These results can be explained by a deficit in time reference as predicted by the PADILIH. Our data reveal that the predictions of the PADILIH also hold for non-fluent speakers who have been diagnosed with Transcortical motor aphasia.}, language = {en} } @article{NeuschaeferRubeLieskeKunaetal.2014, author = {Neuschaefer-Rube, Frank and Lieske, Stefanie and Kuna, Manuela and Henkel, Janin and Perry, Rachel J. and Erion, Derek M. and Pesta, Dominik and Willmes, Diana M. and Brachs, Sebastian and von Loeffelholz, Christian and Tolkachov, Alexander and Schupp, Michael and Pathe-Neuschaefer-Rube, Andrea and Pfeiffer, Andreas F. H. and Shulman, Gerald I. and P{\"u}schel, Gerhard Paul and Birkenfeld, Andreas L.}, title = {The mammalian INDY homolog is induced by CREB in a rat model of type 2 diabetes}, series = {Diabetes : a journal of the American Diabetes Association}, volume = {63}, journal = {Diabetes : a journal of the American Diabetes Association}, number = {3}, publisher = {American Diabetes Association}, address = {Alexandria}, issn = {0012-1797}, pages = {1048 -- 1057}, year = {2014}, language = {en} } @article{IvanovBeninaPetrovetal.2014, author = {Ivanov, Ivan and Benina, Maria and Petrov, Veselin and Gechev, Tsanko S. and Toneva, Valentina}, title = {Metabolic responses of gloxinia perennis to dehydration and rehydration}, series = {COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES}, volume = {67}, journal = {COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES}, number = {12}, publisher = {Publ. House of the Bulgarian Acad. of Sciences}, address = {Sofia}, issn = {1310-1331}, pages = {1657 -- 1662}, year = {2014}, abstract = {Gloxinia perennis is a species from the family Gesneriaceae with little known physiology, particularly in respect to responses to dehydration. G. perennis survived water deprivation for a month and then quickly recovered upon rehydration. The slow loss of water was in contrast with the quick dehydration of other Gesnerian species - Boea hygrometrica, Ramonda serbica, and Haber lea rhodopensis. Furthermore, a significant difference between older and younger leaves of G. perennis was observed. While the relative water content in the early stages of water deprivation was reduced to 65\% in the old leaves, it was not or slightly reduced in the young ones, implying a mechanism that protects specifically the younger leaves from dehydration. Water deprivation induced accumulation of gama-aminobutyric acid and sugars like sucrose and raffinose, but decreased the levels of amino acids such as glycine, leucine, and isoleucine. The levels of these amino acids recovered after rehydration and in some cases like glycine and isoleucine were even higher in rehydrated leaves compared with unstressed controls. We conclude that G.perennis can survive prolonged drought stress but its responses to dehydration are different from the resurrection species from Gesneriaceae. All this makes G. perennis a good model that can be used for comparative genomics and metabolomics of Gesneriads exposed to desiccation.}, language = {en} } @article{Hassler2014, author = {Haßler, Gerda}, title = {Evidentiality and the expression of speaker's stance in Romance languages and German}, series = {Discourse Studies : an interdisciplinary journal for the study of text and talk}, volume = {17}, journal = {Discourse Studies : an interdisciplinary journal for the study of text and talk}, number = {2}, publisher = {Sage Publications}, address = {London}, issn = {1461-4456}, doi = {10.1177/1461445614564522}, pages = {182 -- 209}, year = {2014}, abstract = {In recent years, the category of evidentiality has also come into use for the description of Romance languages and of German. This has been contingent on a change in its interpretation from a typological category to a semantic-pragmatic category, which allows an application to languages lacking specialised morphemes for the expression of evidentiality. We consider evidentiality to be a structural dimension of grammar, the values of which are expressed by types of constructions that code the source of information which a speaker imparts. If we look at the situation in Romance languages and in German, drawing a boundary between epistemic modality and evidentiality presents problems that are difficult to solve. Adding markers of the source of the speaker's knowledge often limits the degree of responsibility of the speaker for the content of the utterance. Evidential adverbs are a frequently used means of marking the source of the speaker's knowledge. The evidential meaning is generalised to marking any source of knowledge, what can be regarded as a result of a process of pragmaticalisation. The use of certain means which also carry out evidential markings can even contribute to the blurring of the different kinds of evidentiality. German also has modal verbs which in conjunction with the perfect tense of the verb have a predominantly evidential use (sollen and wollen). But even here the evidential marking is not without influence on the modality of the utterance. The Romance languages, however, do not have such specialised verbs for expressing evidentiality in certain contexts. To do this, they mark evidentiality - often context bound - by verb forms such as the conditional and the imperfect tense. This article shall contrast the different architectures used in expressing evidentiality in German and in the Romance languages.}, language = {en} } @article{Guenther2014, author = {G{\"u}nther, Oliver}, title = {Big Data: What is it? And what does it mean for science, economy and society?}, series = {Informatik-Spektrum}, volume = {37}, journal = {Informatik-Spektrum}, number = {2}, publisher = {Springer Verlag}, address = {Heidelberg}, issn = {0170-6012}, doi = {10.1007/s00287-014-0783-7}, pages = {85 -- 86}, year = {2014}, language = {en} } @article{HemmeVeyelMuehlhausetal.2014, author = {Hemme, Dorothea and Veyel, Daniel and Muehlhaus, Timo and Sommer, Frederik and Jueppner, Jessica and Unger, Ann-Katrin and Sandmann, Michael and Fehrle, Ines and Schoenfelder, Stephanie and Steup, Martin and Geimer, Stefan and Kopka, Joachim and Giavalisco, Patrick and Schroda, Michael}, title = {Systems-wide analysis of acclimation responses to long-term heat stress and recovery in the photosynthetic model organism Chlamydomonas reinhardtii}, series = {The plant cell}, volume = {26}, journal = {The plant cell}, number = {11}, publisher = {American Society of Plant Physiologists}, address = {Rockville}, issn = {1040-4651}, doi = {10.1105/tpc.114.130997}, pages = {4270 -- 4297}, year = {2014}, abstract = {We applied a top-down systems biology approach to understand how Chlamydomonas reinhardtii acclimates to long-term heat stress (HS) and recovers from it. For this, we shifted cells from 25 to 42 degrees C for 24 h and back to 25 degrees C for >= 8 h and monitored abundances of 1856 proteins/protein groups, 99 polar and 185 lipophilic metabolites, and cytological and photosynthesis parameters. Our data indicate that acclimation of Chlamydomonas to long-term HS consists of a temporally ordered, orchestrated implementation of response elements at various system levels. These comprise (1) cell cycle arrest; (2) catabolism of larger molecules to generate compounds with roles in stress protection; (3) accumulation of molecular chaperones to restore protein homeostasis together with compatible solutes; (4) redirection of photosynthetic energy and reducing power from the Calvin cycle to the de novo synthesis of saturated fatty acids to replace polyunsaturated ones in membrane lipids, which are deposited in lipid bodies; and (5) when sinks for photosynthetic energy and reducing power are depleted, resumption of Calvin cycle activity associated with increased photorespiration, accumulation of reactive oxygen species scavengers, and throttling of linear electron flow by antenna uncoupling. During recovery from HS, cells appear to focus on processes allowing rapid resumption of growth rather than restoring pre-HS conditions.}, language = {en} } @article{CisekTokarzKrouglovetal.2014, author = {Cisek, Richard and Tokarz, Danielle and Krouglov, Serguei and Steup, Martin and Emes, Michael J. and Tetlow, Ian J. and Barzda, Virginijus}, title = {Second harmonic generation mediated by aligned water in starch granules}, series = {The journal of physical chemistry : B, Condensed matter, materials, surfaces, interfaces \& biophysical chemistry}, volume = {118}, journal = {The journal of physical chemistry : B, Condensed matter, materials, surfaces, interfaces \& biophysical chemistry}, number = {51}, publisher = {American Chemical Society}, address = {Washington}, issn = {1520-6106}, doi = {10.1021/jp508751s}, pages = {14785 -- 14794}, year = {2014}, abstract = {The origin of second harmonic generation (SHG) in starch granules was investigated using ab initio quantum mechanical modeling and experimentally examined using polarization-in, polarization-out (PIPO) second harmonic generation microscopy. Ab initio calculations revealed that the largest contribution to the SHG signal from A- and B-type allomorphs of starch originates from the anisotropic organization of hydroxide and hydrogen bonds mediated by aligned water found in the polymers. The hypothesis was experimentally tested by imaging maize starch granules under various hydration and heat treatment conditions that alter the hydrogen bond network. The highest SHG intensity was found in fully hydrated starch granules, and heat treatment diminished the SHG intensity. The PIPO SHG imaging showed that dried starch granules have a much higher nonlinear optical susceptibility component ratio than fully hydrated granules. In contrast, deuterated starch granules showed a smaller susceptibility component ratio demonstrating that SHG is highly sensitive to the organization of the hydroxyl and hydrogen bond network. The polarization SHG imaging results of potato starch granules, representing starch allomorph B, were compared to those of maize starch granules representing allomorph A. The results showed that the amount of aligned water was higher in the maize granules. Nonlinear microscopy of starch granules provides evidence that varying hydration conditions leads to significant changes in the nonlinear susceptibility ratio as well as the SHG intensity, supporting the hypothesis from ab initio calculations that the dominant contribution to SHG is due to the ordered hydroxide and hydrogen bond network.}, language = {en} } @article{TenenboimSmirnovaWillmitzeretal.2014, author = {Tenenboim, Hezi and Smirnova, Julia and Willmitzer, Lothar and Steup, Martin and Brotman, Yariv}, title = {VMP1-deficient Chlamydomonas exhibits severely aberrant cell morphology and disrupted cytokinesies}, series = {BMC plant biology}, volume = {14}, journal = {BMC plant biology}, publisher = {BioMed Central}, address = {London}, issn = {1471-2229}, doi = {10.1186/1471-2229-14-121}, pages = {13}, year = {2014}, abstract = {Background: The versatile Vacuole Membrane Protein 1 (VMP1) has been previously investigated in six species. It has been shown to be essential in macroautophagy, where it takes part in autophagy initiation. In addition, VMP1 has been implicated in organellar biogenesis; endo-, exo- and phagocytosis, and protein secretion; apoptosis; and cell adhesion. These roles underly its proven involvement in pancreatitis, diabetes and cancer in humans. Results: In this study we analyzed a VMP1 homologue from the green alga Chlamydomonas reinhardtii. CrVMP1 knockdown lines showed severe phenotypes, mainly affecting cell division as well as the morphology of cells and organelles. We also provide several pieces of evidence for its involvement in macroautophagy.}, language = {en} } @article{LukasWacker2014, author = {Lukas, Marcus and Wacker, Alexander}, title = {Constraints by oxygen and food quality on carbon pathway regulation: a co-limitation study with an aquatic key herbivore}, series = {Ecology : a publication of the Ecological Society of America}, volume = {95}, journal = {Ecology : a publication of the Ecological Society of America}, number = {11}, publisher = {Wiley}, address = {Washington}, issn = {0012-9658}, pages = {3068 -- 3079}, year = {2014}, abstract = {In food webs, herbivores are often constrained by low food quality in terms of mineral and biochemical limitations, which in aquatic ecosystems can co-occur with limited oxygen conditions. As low food quality implies that carbon (C) is available in excess, and therefore a regulation to get rid of excess C is crucial for the performance of consumers, we examined the C pathways (ingestion, feces release, excretion, and respiration) of a planktonic key herbivore (Daphnia magna). We tested whether consumer C pathways increase due to mineral (phosphorus, P) or biochemical (cholesterol and fatty acid) limitations and how these regulations vary when in addition oxygen is low. Under such conditions, at least the capability of the upregulation of respiration may be restricted. Furthermore, we discussed the potential role of the oxygen-transporting protein hemoglobin (Hb) in the regulation of C budgets. Different food quality constraints led to certain C regulation patterns to increase the removal of excess dietary C: P-limited D. magna increased excretion and respiration, while cholesterol-limited Daphnia in addition upregulated the release of feces. In contrast, the regulative effort was low and only feces release increased when D. magna was limited by a long-chain polyunsaturated fatty acid (eicosapentaenoic acid, EPA). Co-limiting oxygen did not always impact the discharge of excess C. We found the food-quality-induced upregulation of respiration was still present at low oxygen. In contrast, higher excretion of excess C was diminished at low oxygen supply. Besides the effect that the Hb concentration increased under low oxygen, our results indicate a low food-quality-induced increase in the Hb content of the animals. Overall, C budgeting is phenotypically plastic towards different (co-) limiting scenarios. These trigger specific regulation responses that could be the result of evolutionary adaptations.}, language = {en} } @article{SpijkermanStojkovicBeardall2014, author = {Spijkerman, Elly and Stojkovic, Slobodanka and Beardall, John}, title = {CO2 acquisition in Chlamydomonas acidophila is influenced mainly by CO2, not phosphorus, availability}, series = {Photosynthesis research}, volume = {121}, journal = {Photosynthesis research}, number = {2-3}, publisher = {Springer}, address = {Dordrecht}, issn = {0166-8595}, doi = {10.1007/s11120-014-0016-6}, pages = {213 -- 221}, year = {2014}, abstract = {The extremophilic green microalga Chlamydomonas acidophila grows in very acidic waters (pH 2.3-3.4), where CO2 is the sole inorganic carbon source. Previous work has revealed that the species can accumulate inorganic carbon (Ci) and exhibits high affinity CO2 utilization under low-CO2 (air-equilibrium) conditions, similar to organisms with an active CO2 concentrating mechanism (CCM), whereas both processes are down-regulated under high CO2 (4.5 \% CO2) conditions. Responses of this species to phosphorus (Pi)-limited conditions suggested a contrasting regulation of the CCM characteristics. Therefore, we measured external carbonic anhydrase (CA(ext)) activities and protein expression (CAH1), the internal pH, Ci accumulation, and CO2-utilization in cells adapted to high or low CO2 under Pi-replete and Pi-limited conditions. Results reveal that C. acidophila expressed CA(ext) activity and expressed a protein cross-reacting with CAH1 (the CA(ext) from Chlamydomonas reinhardtii). Although the function of this CA remains unclear, CA(ext) activity and high affinity CO2 utilization were the highest under low CO2 conditions. C. acidophila accumulated Ci and expressed the CAH1 protein under all conditions tested, and C. reinhardtii also contained substantial amounts of CAH1 protein under Pi-limitation. In conclusion, Ci utilization is optimized in C. acidophila under ecologically relevant conditions, which may enable optimal survival in its extreme Ci- and Pi-limited habitat. The exact physiological and biochemical acclimation remains to be further studied.}, language = {en} } @article{LukasWacker2014, author = {Lukas, Marcus and Wacker, Alexander}, title = {Daphnia's dilemma: adjustment of carbon budgets in the face of food and cholesterol limitation}, series = {The journal of experimental biology}, volume = {217}, journal = {The journal of experimental biology}, number = {7}, publisher = {Company of Biologists Limited}, address = {Cambridge}, issn = {0022-0949}, doi = {10.1242/jeb.094151}, pages = {1079 -- 1086}, year = {2014}, abstract = {We studied the carbon (C) metabolism in Daphnia when the amount of C (food quantity) and/or the content of biochemical nutrients (food quality) was limiting. Growth performances and C budgets of Daphnia magna (assimilation, faeces egestion, excretion and respiration measured by [C-14]-tracing) were analysed when animals were raised on different food quantities and concentrations of cholesterol, an essential biochemical food compound. Cholesterol is of special interest because it not only acts as limiting nutrient but also contributes to the overall C pool of the animals. As the tissue cholesterol concentration in Daphnia is quite low, we hypothesized the selective exclusion of cholesterol from C budgeting and tested this using radiolabelled cholesterol. Somatic growth rates of D. magna were highest at high quantity and quality and were reduced to a moderate value if either the food quantity or the cholesterol concentration was low. Growth was lowest at low food quantity and quality. The measurements of C budgets revealed high regulative response to low food quality at high food quantity only. Here, low dietary cholesterol caused bulk C assimilation efficiency (AE) to decrease and assimilated (excess) C to be increasingly respired. Additionally, Daphnia enhanced efficient adjustment of C budgets when facing cholesterol limitation by (1) increasing the AE of the cholesterol itself and (2) not changing cholesterol respiration, which was still not detectable. In contrast, at low food quantity, Daphnia is unable to adjust for low food quality, emphasizing that food limitation could overrule food quality effects.}, language = {en} } @article{LukasWacker2014, author = {Lukas, Marcus and Wacker, Alexander}, title = {Acclimation to dietary shifts impacts the carbon budgets of Daphnia magna}, series = {Journal of plankton research}, volume = {36}, journal = {Journal of plankton research}, number = {3}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {0142-7873}, doi = {10.1093/plankt/fbu018}, pages = {848 -- 858}, year = {2014}, abstract = {Daphnia responds to low availability of carbon (food quantity) or limiting concentrations of nutrients relative to carbon (C) in excess (food quality) by respectively saving or discharging C via different pathways. We investigated which kind of food limitation leads to a faster regulation in Daphnia C budgets, and whether the pre-assimilative C pathways, ingestion and faeces egestion and the post-assimilative C pathways, excretion and respiration, are regulated concurrently. Daphnia magna were exposed to dietary shifts in different food quantities or qualities; food quality was varied in terms of the essential component, cholesterol. After acclimation to the new diet ranging from 0 to 96 h, C budgets were measured by a radiotracer technique. Dietary shifts in quantity and quality caused Daphnia to quickly adjust their C budgets within 6 h, but different C pathways were affected. A shift to low food quantity reduced Daphnia respiration indicating C retention. In contrast, sudden low quality food caused increased faeces egestion to discharge excess C. Furthermore, we observed a delayed increase in excretion but no change in respiration within the time frame studied. Such time-shifted responses appear to be an appropriate means to keep the costs of physiological adjustments relatively low, which in turn would benefit Daphnia performance.}, language = {en} } @article{SeifertdeCastroMarquartetal.2014, author = {Seifert, Linda I. and de Castro, Francisco and Marquart, Arnim and Gaedke, Ursula and Weithoff, Guntram and Vos, Matthijs}, title = {Heated relations: temperature-mediated shifts in consumption across trophic levels}, series = {PLoS one}, volume = {9}, journal = {PLoS one}, number = {5}, publisher = {PLoS}, address = {San Fransisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0095046}, pages = {7}, year = {2014}, abstract = {A rise in temperature will intensify the feeding links involving ectotherms in food webs. However, it is unclear how the effects will quantitatively differ between the plant-herbivore and herbivore-carnivore interface. To test how warming could differentially affect rates of herbivory and carnivory, we studied trophic interaction strength in a food chain comprised of green algae, herbivorous rotifers and carnivorous rotifers at 10, 15, 20 and 25 degrees C. We found significant warming-induced changes in feeding by both herbivorous and carnivorous rotifers, but these responses occurred at different parts of the entire temperature gradient. The strongest response of the per capita herbivore's ingestion rate occurred due to an increase in temperature from 15 to 20 degrees C (1.9 fold: from 834 to 1611 algal cells per h(-1)) and of the per capita carnivore's ingestion rate from 20 to 25 degrees C (1.6 fold: from 1.5 to 2.5 prey h(-1)). Handling time, an important component of a consumer's functional response, significantly decreased from 15 to 20 degrees C in herbivorous rotifers. In contrast, it decreased from 20 to 25 degrees C in carnivorous rotifers. Attack rates significantly and strongly increased from 10 to 25 degrees C in the herbivorous animals, but not at all in the carnivores. Our results exemplify how the relative forces of top-down control exerted by herbivores and carnivores may strongly shift under global warming. But warming, and its magnitude, are not the only issue: If our results would prove to be representative, shifts in ectotherm interactions will quantitatively differ when a 5 degrees C increase starts out from a low, intermediate or high initial temperature. This would imply that warming could have different effects on the relative forces of carnivory and herbivory in habitats differing in average temperature, as would exist at different altitudes and latitudes.}, language = {en} } @article{ZimmermannSener2014, author = {Zimmermann, Andreas and Sener, Meltem}, title = {Chemical weapons and the international criminal court}, series = {American journal of international law}, volume = {108}, journal = {American journal of international law}, number = {3}, publisher = {American Society of International Law}, address = {Washington}, issn = {0002-9300}, doi = {10.5305/amerjintelaw.108.3.0436}, pages = {436 -- 448}, year = {2014}, language = {en} } @article{Noble2014, author = {Noble, Andrew}, title = {Ultraschall Berlin}, series = {Tempo: a quarterly review of modern music}, volume = {68}, journal = {Tempo: a quarterly review of modern music}, number = {269}, publisher = {Cambidge University Press}, address = {Cambridge}, issn = {1478-2286}, doi = {10.1017/S004029821400014X}, pages = {76 -- 78}, year = {2014}, language = {en} } @article{Hassler2014, author = {Haßler, Gerda}, title = {Los tiempos verbales y sus denominaciones en las gram{\´a}ticas espa{\~n}olas desde el siglo XVII hasta el siglo XX}, series = {M{\´e}todos y resultados actuales en Historiograf{\´i}a de la Ling{\"u}{\´i}stica}, volume = {1}, journal = {M{\´e}todos y resultados actuales en Historiograf{\´i}a de la Ling{\"u}{\´i}stica}, publisher = {Nodus Publikationen}, address = {M{\"u}nster}, isbn = {978-3-89323-020-4}, pages = {349 -- 360}, year = {2014}, abstract = {In this paper, the names of the Spanish verb forms are examined with emphasis on the forms of the so-called past tense and the relationship of their conceptual potential to the function of these forms. The names of the verb forms in Spanish grammars from the 17th to the 20th century are determined by Latin grammar, but also by theoretical positions of their authors.}, language = {es} } @article{FilipBauerHillebrandetal.2014, author = {Filip, Joanna and Bauer, Barbara and Hillebrand, Helmut and Beniermann, Anna and Gaedke, Ursula and Moorthi, Stefanie D.}, title = {Multitrophic diversity effects depend on consumer specialization and species-specific growth and grazing rates}, series = {Oikos}, volume = {123}, journal = {Oikos}, number = {8}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {0030-1299}, doi = {10.1111/oik.01219}, pages = {912 -- 922}, year = {2014}, abstract = {Ecosystem functioning is affected by horizontal (within trophic groups) and vertical (across trophic levels) biodiversity. Theory predicts that the effects of vertical biodiversity depend on consumer specialization. In a microcosm experiment, we investigated ciliate consumer diversity and specialization effects on algal prey biovolume, evenness and composition, and on ciliate biovolume production. The experimental data was complemented by a process-based model further analyzing the ecological mechanisms behind the observed diversity effects. Overall, increasing consumer diversity had no significant effect on prey biovolume or evenness. However, consumer specialization affected the prey community. Specialist consumers showed a stronger negative impact on prey biovolume and evenness than generalists. The model confirmed that this pattern was mainly driven by a single specialist with a high per capita grazing rate, consuming the two most productive prey species. When these were suppressed, the prey assemblage became dominated by a less productive species, consequently decreasing prey biovolume and evenness. Consumer diversity increased consumer biovolume, which was stronger for generalists than for specialists and highest in mixed combinations, indicating that consumer functional diversity, i.e. more diverse feeding strategies, increased resource use efficiency. Overall, our results indicate that consumer diversity effects on prey and consumers strongly depend on species-specific growth and grazing rates, which may be at least equally important as consumer specialization in driving consumer diversity effects across trophic levels.}, language = {en} } @article{DietrichLombardoAbdelilahSeyfried2014, author = {Dietrich, Ann-Christin and Lombardo, Veronica A. and Abdelilah-Seyfried, Salim}, title = {Blood flow and Bmp signaling control endocardial chamber morphogenesis}, series = {Developmental cell}, volume = {30}, journal = {Developmental cell}, number = {4}, publisher = {Cell Press}, address = {Cambridge}, issn = {1534-5807}, doi = {10.1016/j.devcel.2014.06.020}, pages = {367 -- 377}, year = {2014}, abstract = {During heart development, the onset of heartbeat and blood flow coincides with a ballooning of the cardiac chambers. Here, we have used the zebrafish as a vertebrate model to characterize chamber ballooning morphogenesis of the endocardium, a specialized population of endothelial cells that line the interior of the heart. By combining functional manipulations, fate mapping studies, and high-resolution imaging, we show that endocardial growth occurs without an influx of external cells. Instead, endocardial cell proliferation is regulated, both by blood flow and by Bmp signaling, in a manner independent of vascular endothelial growth factor (VEGF) signaling. Similar to myocardial cells, endocardial cells obtain distinct chamber-specific and inner- versus outer-curvature-specific surface area sizes. We find that the hemodynamic-sensitive transcription factor Klf2a is involved in regulating endocardial cell morphology. These findings establish the endocardium as the flow-sensitive tissue in the heart with a key role in adapting chamber growth in response to the mechanical stimulus of blood flow.}, language = {en} } @article{OtreloCardosodaSilvaCorreiaSchwuchowetal.2014, author = {Otrelo-Cardoso, Ana Rita and da Silva Correia, Marcia Alexandra and Schwuchow, Viola and Svergun, Dmitri I. and Romao, Maria Joao and Leimk{\"u}hler, Silke and Santos-Silva, Teresa}, title = {Structural Data on the Periplasmic Aldehyde Oxidoreductase PaoABC from Escherichia coli: SAXS and Preliminary X-ray Crystallography Analysis}, series = {International journal of molecular sciences}, volume = {15}, journal = {International journal of molecular sciences}, number = {2}, publisher = {MDPI}, address = {Basel}, issn = {1422-0067}, doi = {10.3390/ijms15022223}, pages = {2223 -- 2236}, year = {2014}, abstract = {The periplasmic aldehyde oxidoreductase PaoABC from Escherichia coli is a molybdenum enzyme involved in detoxification of aldehydes in the cell. It is an example of an heterotrimeric enzyme of the xanthine oxidase family of enzymes which does not dimerize via its molybdenum cofactor binding domain. In order to structurally characterize PaoABC, X-ray crystallography and small angle X-ray scattering (SAXS) have been carried out. The protein crystallizes in the presence of 20\% (w/v) polyethylene glycol 3350 using the hanging-drop vapour diffusion method. Although crystals were initially twinned, several experiments were done to overcome twinning and lowering the crystallization temperature (293 K to 277 K) was the solution to the problem. The non-twinned crystals used to solve the structure diffract X-rays to beyond 1.80 angstrom and belong to the C2 space group, with cell parameters a = 109.42 angstrom, b = 78.08 angstrom, c = 151.77 angstrom, = 99.77 degrees, and one molecule in the asymmetric unit. A molecular replacement solution was found for each subunit separately, using several proteins as search models. SAXS data of PaoABC were also collected showing that, in solution, the protein is also an heterotrimer.}, language = {en} } @article{MartinCreuzburgOexleWacker2014, author = {Martin-Creuzburg, Dominik and Oexle, Sarah and Wacker, Alexander}, title = {Thresholds for sterol-limited growth of Daphnia magna: A comparative approach using 10 different sterols}, series = {Journal of chemical ecology}, volume = {40}, journal = {Journal of chemical ecology}, number = {9}, publisher = {Springer}, address = {Dordrecht}, issn = {0098-0331}, doi = {10.1007/s10886-014-0486-1}, pages = {1039 -- 1050}, year = {2014}, abstract = {Arthropods are incapable of synthesizing sterols de novo and thus require a dietary source to cover their physiological demands. The most prominent sterol in animal tissues is cholesterol, which is an indispensable structural component of cell membranes and serves as precursor for steroid hormones. Instead of cholesterol, plants and algae contain a variety of different phytosterols. Consequently, herbivorous arthropods have to metabolize dietary phytosterols to cholesterol to meet their requirements for growth and reproduction. Here, we investigated sterol-limited growth responses of the freshwater herbivore Daphnia magna by supplementing a sterol-free diet with increasing amounts of 10 different phytosterols and comparing thresholds for sterol-limited growth. In addition, we analyzed the sterol composition of D. magna to explore sterol metabolic constraints and bioconversion capacities. We show that dietary phytosterols strongly differ in their potential to support somatic growth of D. magna. The dietary threshold concentrations obtained by supplementing the different sterols cover a wide range (3.5-34.4 mu g mg C-1) and encompass the one for cholesterol (8.9 mu g mg C-1), indicating that certain phytosterols are more efficient in supporting somatic growth than cholesterol (e.g., fucosterol, brassicasterol) while others are less efficient (e.g., dihydrocholesterol, lathosterol). The dietary sterol concentration gradients revealed that the poor quality of particular sterols can be alleviated partially by increasing dietary concentrations, and that qualitative differences among sterols are most pronounced at low to moderate dietary concentrations. We infer that the dietary sterol composition has to be considered in zooplankton nutritional ecology to accurately assess potential sterol limitations under field conditions.}, language = {en} } @article{HovestaedtMemczakPleineretal.2014, author = {Hovestaedt, Marc and Memczak, Henry and Pleiner, Dennis and Zhang, Xin and Rappich, Joerg and Bier, Frank Fabian and St{\"o}cklein, Walter F. M.}, title = {Characterization of a new maleimido functionalization of gold for surface plasmon resonance spectroscopy}, series = {Journal of molecular recognition : an international journal devoted to research on specific molecular recognition in chemistry, biology, biotechnology and medicine}, volume = {27}, journal = {Journal of molecular recognition : an international journal devoted to research on specific molecular recognition in chemistry, biology, biotechnology and medicine}, number = {12}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {0952-3499}, doi = {10.1002/jmr.2396}, pages = {707 -- 713}, year = {2014}, abstract = {Para-maleimidophenyl (p-MP) modified gold surfaces have been prepared by one-step electrochemical deposition and used in surface plasmon resonance (SPR) studies. Therefore, a FITC mimotope peptide (MP1, 12 aa), a human mucin 1 epitope peptide (MUC, 9 aa) and a protein with their specific antibodies were used as model systems. The peptides were modified with an N-terminal cysteine for covalent and directed coupling to the maleimido functionalized surface by means of Michael addition. The coupling yield of the peptide, the binding characteristics of antibody and the unspecific adsorption of the analytes were investigated. The results expand the spectrum of biosensors usable with p-MP by widely used SPR and support its potential to be versatile for several electrochemical and optical biosensors. This allows the combination of an electrochemical and optical read-out for a broad variety of biomolecular interactions on the same chip. Copyright (c) 2014 John Wiley \& Sons, Ltd.}, language = {en} } @article{KerstingRauschBieretal.2014, author = {Kersting, Sebastian and Rausch, Valentina and Bier, Frank Fabian and von Nickisch-Rosenegk, Markus}, title = {Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis}, series = {Malaria journal}, volume = {13}, journal = {Malaria journal}, publisher = {BioMed Central}, address = {London}, issn = {1475-2875}, doi = {10.1186/1475-2875-13-99}, pages = {9}, year = {2014}, abstract = {Background: Nucleic acid amplification is the most sensitive and specific method to detect Plasmodium falciparum. However the polymerase chain reaction remains laboratory-based and has to be conducted by trained personnel. Furthermore, the power dependency for the thermocycling process and the costly equipment necessary for the read-out are difficult to cover in resource-limited settings. This study aims to develop and evaluate a combination of isothermal nucleic acid amplification and simple lateral flow dipstick detection of the malaria parasite for point-of-care testing. Methods: A specific fragment of the 18S rRNA gene of P. falciparum was amplified in 10 min at a constant 38 C using the isothermal recombinase polymerase amplification (RPA) method. With a unique probe system added to the reaction solution, the amplification product can be visualized on a simple lateral flow strip without further labelling. The combination of these methods was tested for sensitivity and specificity with various Plasmodium and other protozoa/bacterial strains, as well as with human DNA. Additional investigations were conducted to analyse the temperature optimum, reaction speed and robustness of this assay. Results: The lateral flow RPA (LF-RPA) assay exhibited a high sensitivity and specificity. Experiments confirmed a detection limit as low as 100 fg of genomic P. falciparum DNA, corresponding to a sensitivity of approximately four parasites per reaction. All investigated P. falciparum strains (n = 77) were positively tested while all of the total 11 non-Plasmodium samples, showed a negative test result. The enzymatic reaction can be conducted under a broad range of conditions from 30-45 degrees C with high inhibitory concentration of known PCR inhibitors. A time to result of 15 min from start of the reaction to read-out was determined. Conclusions: Combining the isothermal RPA and the lateral flow detection is an approach to improve molecular diagnostic for P. falciparum in resource-limited settings. The system requires none or only little instrumentation for the nucleic acid amplification reaction and the read-out is possible with the naked eye. Showing the same sensitivity and specificity as comparable diagnostic methods but simultaneously increasing reaction speed and dramatically reducing assay requirements, the method has potential to become a true point-of-care test for the malaria parasite.}, language = {en} } @article{HoppeBiervonNickischRosenegk2014, author = {Hoppe, Sebastian and Bier, Frank Fabian and von Nickisch-Rosenegk, Markus}, title = {Identification of antigenic proteins of the nosocomial pathogen klebsiella pneumoniae}, series = {PLoS one}, volume = {9}, journal = {PLoS one}, number = {10}, publisher = {PLoS}, address = {San Fransisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0110703}, pages = {16}, year = {2014}, abstract = {The continuous expansion of nosocomial infections around the globe has become a precarious situation. Key challenges include mounting dissemination of multiple resistances to antibiotics, the easy transmission and the growing mortality rates of hospital-acquired bacterial diseases. Thus, new ways to rapidly detect these infections are vital. Consequently, researchers around the globe pursue innovative approaches for point-of-care devices. In many cases the specific interaction of an antigen and a corresponding antibody is pivotal. However, the knowledge about suitable antigens is lacking. The aim of this study was to identify novel antigens as specific diagnostic markers. Additionally, these proteins might be aptly used for the generation of vaccines to improve current treatment options. Hence, a cDNA-based expression library was constructed and screened via microarrays to detect novel antigens of Klebsiella pneumoniae, a prominent agent of nosocomial infections well-known for its extensive antibiotics resistance, especially by extended-spectrum beta-lactamases (ESBL). After screening 1536 clones, 14 previously unknown immunogenic proteins were identified. Subsequently, each protein was expressed in full-length and its immunodominant character examined by ELISA and microarray analyses. Consequently, six proteins were selected for epitope mapping and three thereof possessed linear epitopes. After specificity analysis, homology survey and 3d structural modelling, one epitope sequence GAVVALSTTFA of KPN_00363, an ion channel protein, was identified harboring specificity for K. pneumoniae. The remaining epitopes showed ambiguous results regarding the specificity for K. pneumoniae. The approach adopted herein has been successfully utilized to discover novel antigens of Campylobacter jejuni and Salmonella enterica antigens before. Now, we have transferred this knowledge to the key nosocomial agent, K. pneumoniae. By identifying several novel antigens and their linear epitope sites, we have paved the way for crucial future research and applications including the design of point-of-care devices, vaccine development and serological screenings for a highly relevant nosocomial pathogen.}, language = {en} } @article{DechtriratGajovicEichelmannWojciketal.2014, author = {Dechtrirat, Decha and Gajovic-Eichelmann, Nenad and Wojcik, Felix and Hartmann, Laura and Bier, Frank Fabian and Scheller, Frieder W.}, title = {Electrochemical displacement sensor based on ferrocene boronic acid tracer and immobilized glycan for saccharide binding proteins and E. coli}, series = {Biosensors and bioelectronics : the principal international journal devoted to research, design development and application of biosensors and bioelectronics}, volume = {58}, journal = {Biosensors and bioelectronics : the principal international journal devoted to research, design development and application of biosensors and bioelectronics}, publisher = {Elsevier}, address = {Oxford}, issn = {0956-5663}, doi = {10.1016/j.bios.2014.02.028}, pages = {1 -- 8}, year = {2014}, abstract = {Pathogens such as viruses and bacteria use their envelope proteins and their adhesin lectins to recognize the glycan residues presented on the cell surface of the target tissues. This principle of recognition is used in a new electrochemical displacement sensor for the protein concanavalin A (ConA). A gold electrode was first modified with a self-assembled monolayer of a thiolated mannose/OEG conjugate and a ferrocene boroxol derivative was pre-assembled as reporter molecule onto the mannose surface. The novel tracer molecule based on a 2-hydroxymethyl phenyl boronic acid derivative binds even at neutral pH to the saccharides which could expand the application towards biological samples (i.e., urine and feces). Upon the binding of ConA, the tracer was displaced and washed away from the sensor surface leading to a decrease in the electrochemical signal. Using square wave voltammetry (SWV), the concentration of ConA in the sample solution could be determined in the dynamic concentration range established from 38 nmol L-1 to 5.76 mu mol L-1 with a reproducible detection limit of 1 mu g mL(-1) (38 nmol L-1) based on the signal-to-noise ratio (S/N=3) with fast response of 15 min. The new reporter molecule showed a reduced non-specific displacement by BSA and ribonuclease A. The sensor was also successfully transferred to the first proof of principle for the detection of Escherichia coli exhibiting a detection limit of approximately 6 x 102 cells/mL Specificity of the displacement by target protein ConA and E. coli was demonstrated since the control proteins (i.e., BSA and RNaseA) and the control E. coli strain, which lack of type 1 fimbriae, were ineffective. (C) 2014 Elsevier B.V. All rights reserved.}, language = {en} } @article{SchellerYarmanBachmannetal.2014, author = {Scheller, Frieder W. and Yarman, Aysu and Bachmann, Till and Hirsch, Thomas and Kubick, Stefan and Renneberg, Reinhard and Schumacher, Soeren and Wollenberger, Ursula and Teller, Carsten and Bier, Frank Fabian}, title = {Future of biosensors: a personal view}, series = {Advances in biochemical engineering, biotechnology}, volume = {140}, journal = {Advances in biochemical engineering, biotechnology}, editor = {Gu, MB and Kim, HS}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-642-54143-8; 978-3-642-54142-1}, issn = {0724-6145}, doi = {10.1007/10_2013_251}, pages = {1 -- 28}, year = {2014}, abstract = {Biosensors representing the technological counterpart of living senses have found routine application in amperometric enzyme electrodes for decentralized blood glucose measurement, interaction analysis by surface plasmon resonance in drug development, and to some extent DNA chips for expression analysis and enzyme polymorphisms. These technologies have already reached a highly advanced level and need minor improvement at most. The dream of the "100-dollar' personal genome may come true in the next few years provided that the technological hurdles of nanopore technology or of polymerase-based single molecule sequencing can be overcome. Tailor-made recognition elements for biosensors including membrane-bound enzymes and receptors will be prepared by cell-free protein synthesis. As alternatives for biological recognition elements, molecularly imprinted polymers (MIPs) have been created. They have the potential to substitute antibodies in biosensors and biochips for the measurement of low-molecular-weight substances, proteins, viruses, and living cells. They are more stable than proteins and can be produced in large amounts by chemical synthesis. Integration of nanomaterials, especially of graphene, could lead to new miniaturized biosensors with high sensitivity and ultrafast response. In the future individual therapy will include genetic profiling of isoenzymes and polymorphic forms of drug-metabolizing enzymes especially of the cytochrome P450 family. For defining the pharmacokinetics including the clearance of a given genotype enzyme electrodes will be a useful tool. For decentralized online patient control or the integration into everyday "consumables' such as drinking water, foods, hygienic articles, clothing, or for control of air conditioners in buildings and cars and swimming pools, a new generation of "autonomous' biosensors will emerge.}, language = {en} } @article{KerstingRauschBieretal.2014, author = {Kersting, Sebastian and Rausch, Valentina and Bier, Frank Fabian and von Nickisch-Rosenegk, Markus}, title = {Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens}, series = {Microchimica acta : analytical sciences based on micro- and nanomaterials}, volume = {181}, journal = {Microchimica acta : analytical sciences based on micro- and nanomaterials}, number = {13-14}, publisher = {Springer}, address = {Wien}, issn = {0026-3672}, doi = {10.1007/s00604-014-1198-5}, pages = {1715 -- 1723}, year = {2014}, abstract = {We report on the development of an on-chip RPA (recombinase polymerase amplification) with simultaneous multiplex isothermal amplification and detection on a solid surface. The isothermal RPA was applied to amplify specific target sequences from the pathogens Neisseria gonorrhoeae, Salmonella enterica and methicillin-resistant Staphylococcus aureus (MRSA) using genomic DNA. Additionally, a positive plasmid control was established as an internal control. The four targets were amplified simultaneously in a quadruplex reaction. The amplicon is labeled during on-chip RPA by reverse oligonucleotide primers coupled to a fluorophore. Both amplification and spatially resolved signal generation take place on immobilized forward primers bount to expoxy-silanized glass surfaces in a pump-driven hybridization chamber. The combination of microarray technology and sensitive isothermal nucleic acid amplification at 38 degrees C allows for a multiparameter analysis on a rather small area. The on-chip RPA was characterized in terms of reaction time, sensitivity and inhibitory conditions. A successful enzymatic reaction is completed in < 20 min and results in detection limits of 10 colony-forming units for methicillin-resistant Staphylococcus aureus and Salmonella enterica and 100 colony-forming units for Neisseria gonorrhoeae. The results show this method to be useful with respect to point-of-care testing and to enable simplified and miniaturized nucleic acid-based diagnostics.}, language = {en} } @article{KedrackiChekiniMaronietal.2014, author = {Kedracki, Dawid and Chekini, Mahshid and Maroni, Plinio and Schlaad, Helmut and Nardin, Corinne}, title = {Synthesis and Self-Assembly of a DNA Molecular Brush}, series = {Biomacromolecules : an interdisciplinary journal focused at the interface of polymer science and the biological sciences}, volume = {15}, journal = {Biomacromolecules : an interdisciplinary journal focused at the interface of polymer science and the biological sciences}, number = {9}, publisher = {American Chemical Society}, address = {Washington}, issn = {1525-7797}, doi = {10.1021/bm5008713}, pages = {3375 -- 3382}, year = {2014}, abstract = {We report herein on the polymer-crystallization-assisted thiol-ene photosynthesis of an amphiphilic comb/graft DNA copolymer, or molecular brush, composed of a hydrophobic poly(2-oxazoline) backbone and hydrophilic short single-stranded nucleic acid grafts. Coupling efficiencies are above 60\% and thus higher as compared with the straight solid-phase-supported synthesis of amphiphilic DNA block copolymers. The DNA molecular brushes self-assemble into sub-micron-sized spherical structures in water as evidenced by light scattering as well as atomic force and electron microscopy imaging. The nucleotide sequences remain functional, as assessed by UV and fluorescence spectroscopy subsequent to isoindol synthesis at the surface of the structures. The determination of a vesicular morphology is supported by encapsulation and subsequent spectroscopy monitoring of the release of a water-soluble dye and spectroscopic quantification of the hybridization efficiency (30\% in average) of the functional nucleic acid strands engaged in structure formation: about one-half of the nucleotide sequences are available for hybridization, whereas the other half are hindered within the self-assembled structure. Because speciation between complementary and non complementary sequences in the medium could be ascertained by confocal laser scanning microscopy, the stable self-assembled molecular brushes demonstrate the potential for sensing applications.}, language = {en} } @article{ZhaoDunlopQiuetal.2014, author = {Zhao, Qiang and Dunlop, John William Chapman and Qiu, Xunlin and Huang, Feihe and Zhang, Zibin and Heyda, Jan and Dzubiella, Joachim and Antonietti, Markus and Yuan, Jiayin}, title = {An instant multi-responsive porous polymer actuator driven by solvent molecule sorption}, series = {Nature Communications}, volume = {5}, journal = {Nature Communications}, publisher = {Nature Publ. Group}, address = {London}, issn = {2041-1723}, doi = {10.1038/ncomms5293}, pages = {8}, year = {2014}, abstract = {Fast actuation speed, large-shape deformation and robust responsiveness are critical to synthetic soft actuators. A simultaneous optimization of all these aspects without trade-offs remains unresolved. Here we describe porous polymer actuators that bend in response to acetone vapour (24 kPa, 20 degrees C) at a speed of an order of magnitude faster than the state-of-the-art, coupled with a large-scale locomotion. They are meanwhile multi-responsive towards a variety of organic vapours in both the dry and wet states, thus distinctive from the traditional gel actuation systems that become inactive when dried. The actuator is easy-to-make and survives even after hydrothermal processing (200 degrees C, 24 h) and pressing-pressure (100 MPa) treatments. In addition, the beneficial responsiveness is transferable, being able to turn 'inert' objects into actuators through surface coating. This advanced actuator arises from the unique combination of porous morphology, gradient structure and the interaction between solvent molecules and actuator materials.}, language = {en} } @article{PiekarczykBaldFlosadottiretal.2014, author = {Piekarczyk, Andreas and Bald, Ilko and Flosadottir, Helga D. and Omarsson, Benedikt and Lafosse, Anne and Ingolfsson, Oddur}, title = {Influence of metal ion complexation on the metastable fragmentation of DNA hexamers}, series = {The European physical journal : D, Atomic, molecular, optical and plasma physics}, volume = {68}, journal = {The European physical journal : D, Atomic, molecular, optical and plasma physics}, number = {6}, publisher = {Springer}, address = {New York}, issn = {1434-6060}, doi = {10.1140/epjd/e2014-40838-7}, pages = {7}, year = {2014}, abstract = {Here, we study the metastable decay of 5'-d(TTGCTT) in the presence of 0-6 alkaline metal ions (Li+, Na+, K+, Rb+) and 0-3 alkaline earth metal ions (Mg2+ and Ca2+), which replace the corresponding number of protons in the oligonucleotide. We find that all ions studied here stabilize the oligonucleotide with respect to simple 3'-C-O backbone cleavage, but at the same time these metal ions promote a central oligonucleotide deletion accompanied by a concomitant recombination of the terminal d(TT) groups. We find that the quenching of the 3'-C-O backbone cleavage is not ion specific, since it is due to the removal of the phosphate protons upon replacement with the respective metal ions. The central nucleotide deletion competes with the 3'-C-O backbone cleavage channels and is thus promoted through the replacement of the exchangeable protons against metal ions. However, with increasing positive charge density of the metal ions the yield of the central nucleotide deletion further increases. We attribute this effect to the necessity of sufficient proximity of the terminal d(TT) group to allow for their recombination on this reaction path. Hence, the formation of a reactive conformer is mediated by the metal ions.}, language = {en} } @article{KopyraKellerBald2014, author = {Kopyra, Janina and Keller, A. and Bald, Ilko}, title = {On the role of fluoro-substituted nucleosides in DNA radiosensitization for tumor radiation therapy}, series = {RSC Advances}, volume = {4}, journal = {RSC Advances}, number = {13}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {2046-2069}, doi = {10.1039/c3ra46735j}, pages = {6825 -- 6829}, year = {2014}, language = {en} } @article{ZhangZhangYouetal.2014, author = {Zhang, Xiaoqing and Zhang, Xinwu and You, Qiong and Sessler, Gerhard M.}, title = {Low- cost, large- area, stretchable piezoelectric films based on irradiation- crosslinked poly ( propylene)}, series = {Macromolecular materials and engineering}, volume = {299}, journal = {Macromolecular materials and engineering}, number = {3}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1438-7492}, doi = {10.1002/mame.201300161}, pages = {290 -- 295}, year = {2014}, abstract = {Low cost, large area, lightweight, stretchable piezoelectric films, based on space-charge electret with a foam structure (i.e., ferroelectrets or piezoelectrets), have been fabricated by using commercially available irradiation cross-linked poly(propylene) (IXPP) foam sheets. Piezoelectric d(33) coefficients are as high as 100pCN(-1). The piezoelectric performance in such IXPP films is well preserved for repeated strains of less than 10\%. Piezoelectric d(33) coefficients are frequency independent in the range from 2 to 100Hz. Such new class materials may be applied in sensory skins, smart clothing, bio-inspired systems, microenergy harvesters, and so on.}, language = {en} } @article{OtreloCardosoSchwuchowRodriguesetal.2014, author = {Otrelo-Cardoso, Ana Rita and Schwuchow, Viola and Rodrigues, David and Cabrita, Eurico J. and Leimk{\"u}hler, Silke and Romao, Maria Joao and Santos-Silva, Teresa}, title = {Biochemical, stabilization and crystallization studies on a molecular chaperone (PaoD) involved in the maturation of molybdoenzymes}, series = {PLoS one}, volume = {9}, journal = {PLoS one}, number = {1}, publisher = {PLoS}, address = {San Fransisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0087295}, pages = {9}, year = {2014}, abstract = {Molybdenum and tungsten enzymes require specific chaperones for folding and cofactor insertion. PaoD is the chaperone of the periplasmic aldehyde oxidoreductase PaoABC. It is the last gene in the paoABCD operon in Escherichia coli and its presence is crucial for obtaining mature enzyme. PaoD is an unstable, 35 kDa, protein. Our biochemical studies showed that it is a dimer in solution with a tendency to form large aggregates, especially after freezing/thawing cycles. In order to improve stability, PaoD was thawed in the presence of two ionic liquids [C(4)mim]Cl and [C(2)OHmim]PF6 and no protein precipitation was observed. This allowed protein concentration and crystallization using polyethylene glycol or ammonium sulfate as precipitating agents. Saturation transfer difference - nuclear magnetic resonance (STD-NMR) experiments have also been performed in order to investigate the effect of the ionic liquids in the stabilization process, showing a clear interaction between the acidic ring protons of the cation and, most likely, negatively charged residues at the protein surface. DLS assays also show a reduction of the overall size of the protein aggregates in presence of ionic liquids. Furthermore, cofactor binding studies on PaoD showed that the protein is able to discriminate between molybdenum and tungsten bound to the molybdenum cofactor, since only a Mo-MPT form of the cofactor remained bound to PaoD.}, language = {en} }