@article{RietdorfBlenauWalz2005,
  author    = {Rietdorf, Katja and Blenau, Wolfgang and Walz, Bernd},
  title     = {Protein secretion in cockroach salivary glands requires an increase in intracellular cAMP and Ca2+ concentrations},
  issn      = {0022-1910},
  year      = {2005},
  abstract  = {The salivary glands in the cockroach Periplaneta americana secrete protein-containing saliva when stimulated by serotonin (5-HT) and protein-free saliva upon dopamine stimulation. In order to obtain information concerning the signalling pathways involved in 5-HT-induced protein secretion, we have determined the protein content of saliva secreted after experimental manipulations that potentially elevate intracellular Ca2+ and cyclic nucleotide concentrations in isolated glands. We have found that 5-HT stimulates the rate of protein secretion in a dose-dependent manner (threshold: 3 x 10(-8) M; EC50 1.5 x 10(-6) M). The maximal rate of 5-HT-induced protein secretion was 2.2 +/- 0.2 mu g/min. Increasing intracellular Ca2+ or cAMP by bath application of ionomycin (5 mu M), db cAMP (10 mM), forskolin (100 mu M) or IBMX (100 mu M), respectively, stimulated protein secretion at significantly lower rates, whereas db cGMP (1 mM) did not activate protein secretion. The high rates and the kinetics of 5-HT-induced protein secretion could only be mimicked by either applying forskolin together with IBMX (with or without ionomycin) or by applying IBMX together with ionomycin. Our measurements suggest that 5-HT-induced protein secretion is mediated by an elevation of [cAMP](i) and that Ca2+ may function as a co-agonist and augment the rate of protein secretion. (c) 2005 Elsevier Ltd. All rights reserved},
  language  = {en}
}
@article{BogenBenderSchlenstedtetal.2005,
  author    = {Bogen, Oliver and Bender, Olaf and Schlenstedt, Jana and Goswami, C. and Schroder, W. and Blenau, Wolfgang and Gillen, C. and Dreger, M. and Hucho, F.},
  title     = {Neuronal expression of the IB4-binding versican},
  issn      = {0022-3042},
  year      = {2005},
  language  = {en}
}
@article{BlenauBaumann2005,
  author    = {Blenau, Wolfgang and Baumann, Arnd},
  title     = {Molecular characterization of the ebony gene from the American cockroach, Periplaneta americana},
  year      = {2005},
  abstract  = {Biogenic amines are an important class of primary messengers in the central (CNS) and peripheral nervous systems and in peripheral organs. These substances regulate and modulate many physiological and behavioral processes. Various inactivation mechanisms for these substances exist to terminate biogenic amine-mediated signal transduction. In vertebrates, the enzymes monoamine oxidase and/or catechol-O-methyl-transferase are involved in these processes. In insects, however, in which both enzymes are low in abundance or absent, biogenic amines are inactivated mainly by N- acetylation or O-sulphation. In Droso-philo, beta-alanyl conjugation mediated by the Ebony protein has recently been shown to be a novel and alternative pathway for biogenic amine inactivation. Here, we report the cloning of ebony cDNA (Peaebony) from a brain-specific cDNA library of the cockroach Periplaneta americana. The open reading frame encodes a protein of 860 amino acid residues (PeaEbony). The PeaEbony polypeptide shares homology to Ebony sequences from Anopheles gambiae, Apis mellifera, and Drosophila melonogaster. In addition, PeaEbony exhibits sequence similarity to a family of microbial non-ribosomal peptide synthetases. The mRNA encoding PeaEbony is highly expressed in the cockroach brain and to a lesser extent in the salivary glands. PeaEbony is, therefore, probably involved in the inactivation of various biogenic amines through beta-alanyl conjugation in the cockroach CNS. Since the salivary glands in Periplaneta are innervated by dopaminergic and serotonergic neurons, PeaEbony probably also biochemically modifies dopamine and serotonin in these acinar glands. Arch. Insect Biochem. (c) 2005 Wiley-Liss, Inc},
  language  = {en}
}
@article{Blenau2005,
  author    = {Blenau, Wolfgang},
  title     = {Cellular actions of biogenic amines},
  year      = {2005},
  language  = {en}
}
@article{MustardKurshanHamiltonetal.2005,
  author    = {Mustard, J. A. and Kurshan, P. T. and Hamilton, Ingrid S. and Blenau, Wolfgang and Mercer, Alison R.},
  title     = {Developmental expression of a tyramine receptor gene in the brain of the honey bee, Apis mellifera},
  year      = {2005},
  language  = {en}
}