@article{BartelHartmannLehmannetal.2012,
  author    = {Bartel, Manuela and Hartmann, Stefanie and Lehmann, Karola and Postel, Kai and Quesada, Humberto and Philipp, Eva E. R. and Heilmann, Katja and Micheel, Burkhard and Stuckas, Heiko},
  title     = {Identification of sperm proteins as candidate biomarkers for the analysis of reproductive isolation in Mytilus: a case study for the enkurin locus},
  series = {Marine biology : international journal on life in oceans and coastal waters},
  volume    = {159},
  journal   = {Marine biology : international journal on life in oceans and coastal waters},
  number    = {10},
  publisher = {Springer},
  address   = {New York},
  issn      = {0025-3162},
  doi       = {10.1007/s00227-012-2005-7},
  pages     = {2195 -- 2207},
  year      = {2012},
  abstract  = {Sperm proteins of the marine sessile mussels of the Mytilus edulis species complex are models to investigate reproductive isolation and speciation. This study aimed at identifying sperm proteins and their corresponding genes. This was aided by the use of monoclonal antibodies that preferentially bind to yet unknown sperm molecules. By identifying their target molecules, this approach identified proteins with relevance to Mytilus sperm function. This procedure identified 16 proteins, for example, enkurin, laminin, porin and heat shock proteins. The potential use of these proteins as genetic markers to study reproductive isolation is exemplified by analysing the enkurin locus. Enkurin evolution is driven by purifying selection, the locus displays high levels of intraspecific variation and species-specific alleles group in distinct phylogenetic clusters. These findings characterize enkurin as informative candidate biomarker for analyses of clinal variation and differential introgression in hybrid zones, for example, to understand determinants of reproductive isolation in Baltic Mytilus populations.},
  language  = {en}
}
@article{StuckasStoofQuesadaetal.2009,
  author    = {Stuckas, Heiko and Stoof, Kathleen and Quesada, Humberto and Tiedemann, Ralph},
  title     = {Evolutionary implications of discordant clines across the Baltic Mytilus hybrid zone (Mytilus edulis and Mytilus trossulus)},
  issn      = {0018-067X},
  doi       = {10.1038/Hdy.2009.37},
  year      = {2009},
  abstract  = {It is well established that reproductive isolation often arises from genome incompatibilities and that genes encoding reproductive traits are less prone to introgression. Hybrid zones of Mytilus trossulus and Mytilus edulis provide an intriguing model to assess reproductive isolation. Although gene flow is restricted in North America, introgression is pervasive in the Baltic. This study aimed at analyzing the shape of multilocus clines across the Baltic contact zone between M. edulis and M. trossulus to infer mechanisms of restriction to gene flow. We use maximum likelihood methods to construct the best fitting individual clines for five markers located on biparentally inherited autosomes and paternally and maternally inherited mitochondrial DNA (mtDNA). Strong cline shape differences among markers suggest that reproductive isolation arising from genome-wide incompatibilities is weak, and that these discrepancies possibly result from genetic drift, hybrid zone movement or marker-specific selection. However, the finding of a common cline center for M7 lysin (involved in fertilization) and paternally transmitted mtDNA (causing nuclear-mitochondrial incompatibilities in hybrids) suggest that these loci may play a role in incomplete reproductive isolation.},
  language  = {en}
}