@article{KabaMaierSchliebeOhleretal.2015, author = {Kaba, Hani E. J. and Maier, Natalia and Schliebe-Ohler, Nicole and Mayer, Yvonne and Mueller, Peter P. and van den Heuvel, Joop and Schuchhardt, Johannes and Hanack, Katja and Bilitewski, Ursula}, title = {Identification of whole pathogenic cells by monoclonal antibodies generated against a specific peptide from an immunogenic cell wall protein}, series = {Journal of microbiological methods}, volume = {108}, journal = {Journal of microbiological methods}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0167-7012}, doi = {10.1016/j.mimet.2014.11.003}, pages = {61 -- 69}, year = {2015}, abstract = {We selected the immunogenic cell wall beta-(1,3)-glucosyltransferase Bgl2p from Candida albicans as a target protein for the production of antibodies. We identified a unique peptide sequence in the protein and generated monoclonal anti- C. albicans Bgl2p antibodies, which bound in particular to whole C. albicans cells.}, language = {en} } @article{HildebrandtGeissler2012, author = {Hildebrandt, Niko and Geissler, Daniel}, title = {Semiconductor quantum dots as FRET acceptors for multiplexed diagnostics and molecular ruler application}, series = {Advances in Experimental Medicine and Biology}, volume = {733}, journal = {Advances in Experimental Medicine and Biology}, editor = {Zahavy, E and Ordentlich, A and Yitzhaki, S and Shafferman, A}, publisher = {Springer}, address = {Dordrecht}, isbn = {978-94-007-2554-6}, issn = {0065-2598}, doi = {10.1007/978-94-007-2555-3_8}, pages = {75 -- 86}, year = {2012}, abstract = {Applications based on Forster resonance energy transfer (FRET) play an important role for the determination of concentrations and distances within nanometer-scale systems in vitro and in vivo in many fields of biotechnology. Semiconductor nanocrystals (Quantum dots - QDs) possess ideal properties for their application as FRET acceptors when the donors have long excited state lifetimes and when direct excitation of QDs can be efficiently suppressed. Therefore, luminescent terbium complexes (LTCs) with excited state lifetimes of more than 2 ms are ideal FRET donor candidates for QD-acceptors. This chapter will give a short overview of theoretical and practical background of FRET, QDs and LTCs, and present some recent applications of LTC-QD FRET pairs for multiplexed ultra-sensitive in vitro diagnostics and nanometer-resolution molecular distance measurements.}, language = {en} } @article{LorenzKleberBuettneretal.2013, author = {Lorenz, A. A. and Kleber, B. and B{\"u}ttner, Michael and Fuchs, M. and M{\"u}rbe, D. and Richter, B. and Sandel, M. and Nawka, T.}, title = {Validierung des Singing Voice Handicap Index in der deutschen Fassung}, series = {HNO}, volume = {61}, journal = {HNO}, number = {8}, publisher = {Springer}, address = {New York}, issn = {0017-6192}, doi = {10.1007/s00106-013-2721-4}, pages = {699 -- 706}, year = {2013}, abstract = {Der Singing Voice Handicap Index (SVHI) wurde zur Selbstbeurteilung einer Stimmst{\"o}rung f{\"u}r S{\"a}nger in den USA entwickelt. Eine deutsche {\"U}bersetzung wurde erstellt und einer Reliabilit{\"a}ts- und Validit{\"a}tspr{\"u}fung unterzogen. Es wurden 54 dysphone S{\"a}nger (35 weiblich, 19 m{\"a}nnlich), Patienten einer phoniatrischen Klinik, befragt. 130 stimmgesunde Opern- und Rundfunkchors{\"a}nger (74 weiblich, 56 m{\"a}nnlich) bildeten die Kontrollgruppe. Die Reliabilit{\"a}t ergibt sich aus einer hochsignifikanten Test-Retest-Reliabilit{\"a}t (r = 0,960; p <= 0,001, Pearson-Korrelation) und einem Cronbach-? von 0,975. Eine Hauptkomponentenanalyse mit Varimaxrotation und die Ergebnisse des Screeplots legen die Interpretation des SVHI als einfaktorielle Skala nahe. Die Validit{\"a}t zeigt sich in einem hochsignifikanten Zusammenhang zwischen dem vom Patienten selbst eingesch{\"a}tzten Schweregrad der Stimmst{\"o}rung und dem SVHI-Gesamtscore. Patienten haben einen signifikant h{\"o}heren SVHI-Gesamtscore als die Kontrollgruppe gesunder S{\"a}nger. Der SVHI ist als diagnostisches Instrument f{\"u}r den deutschsprachigen Raum geeignet.}, language = {de} }