@article{AwaisAhmadKhanetal.2018,
  author    = {Awais, Muhammad and Ahmad, Rafiq and Khan, Nadeem and Garapati, Prashanth and Shahzad, Muhammad and Afroz, Amber and Rashid, Umer and Khan, Sabaz Ali},
  title     = {Transformation of tomato variety rio grande with drought resistant transcription factor gene ATAF1 and its molecular analysis},
  series = {Pakistan Journal of Botany},
  volume    = {50},
  journal   = {Pakistan Journal of Botany},
  number    = {5},
  publisher = {Pakistan botanic soc},
  address   = {Karachi},
  issn      = {0556-3321},
  pages     = {1811 -- 1820},
  year      = {2018},
  abstract  = {Tomato (Solanum lycopersicum L.) being an important vegetable is cultivated and used throughout the world. It not only contributes in fulfilling the basic nutritional requirements of the human body but also has many health benefits due to its rich biochemical composition. However, its production at large scale is hampered by many limiting factors such as biotic and abiotic stresses. Among the different abiotic stresses, drought poses drastic impact on tomato yield. Drought stress is genetically regulated by many transcription factors that not only regulate the stress responsive mechanism but also facilitate the growth and development of tomato plants. NAC is an important stress related transcription factor genes family, and the ATAF1 gene, a member of this family, is involved in ABA signaling and stress response. In this study, tomato variety Rio Drande was transformed with drought resistant ATAF1 gene via Agrobacterium mediated gene transformation method. The ATAF1 gene was first cloned in the pK7WFG2 vector having kanamycin selectable marker and then it was introduced in the Agrobacterium tumefaciens strain GV3101 through heat shock method. The tomato cotyledon and hypocotyl ex-plants of variety "Rio Ggrande" were cultured on callus induction medium (MS + 2.5 mg/L IAA + 2 mg/L BAP). The calli were then infected with Agrobacterium tumefaciens strain GV3101 containing ATAF1 gene and selection was carried out on the kanamycin selectable medium (MS + 100 mg/L Kan), and were regenerated on MS medium with 1 mg/L IAA + 1 mg/L BAP. Out of 216 putative transformed calli, 13 calli were able to regenerate on the selection medium. Of the 13 calli, three transgenic tomato plantlets were recovered, and these were confirmed through PCR analysis for the presence of 432 bp fragment of ATAF1 gene. The transformation protocol reported here can be used to generate drought resistant tomato plants in future.},
  language  = {en}
}
@article{IvanovBeninaPetrovetal.2014,
  author    = {Ivanov, Ivan and Benina, Maria and Petrov, Veselin and Gechev, Tsanko S. and Toneva, Valentina},
  title     = {Metabolic responses of gloxinia perennis to dehydration and rehydration},
  series = {COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES},
  volume    = {67},
  journal   = {COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES},
  number    = {12},
  publisher = {Publ. House of the Bulgarian Acad. of Sciences},
  address   = {Sofia},
  issn      = {1310-1331},
  pages     = {1657 -- 1662},
  year      = {2014},
  abstract  = {Gloxinia perennis is a species from the family Gesneriaceae with little known physiology, particularly in respect to responses to dehydration. G. perennis survived water deprivation for a month and then quickly recovered upon rehydration. The slow loss of water was in contrast with the quick dehydration of other Gesnerian species - Boea hygrometrica, Ramonda serbica, and Haber lea rhodopensis. Furthermore, a significant difference between older and younger leaves of G. perennis was observed. While the relative water content in the early stages of water deprivation was reduced to 65\% in the old leaves, it was not or slightly reduced in the young ones, implying a mechanism that protects specifically the younger leaves from dehydration. Water deprivation induced accumulation of gama-aminobutyric acid and sugars like sucrose and raffinose, but decreased the levels of amino acids such as glycine, leucine, and isoleucine. The levels of these amino acids recovered after rehydration and in some cases like glycine and isoleucine were even higher in rehydrated leaves compared with unstressed controls. We conclude that G.perennis can survive prolonged drought stress but its responses to dehydration are different from the resurrection species from Gesneriaceae. All this makes G. perennis a good model that can be used for comparative genomics and metabolomics of Gesneriads exposed to desiccation.},
  language  = {en}
}