@article{StreubelFritzTeltowetal.2018,
  author    = {Streubel, Susanna and Fritz, Michael Andre and Teltow, Melanie and Kappel, Christian and Sicard, Adrien},
  title     = {Successive duplication-divergence mechanisms at the RCO locus contributed to leaf shape diversity in the Brassicaceae},
  series = {Development : Company of Biologists},
  volume    = {145},
  journal   = {Development : Company of Biologists},
  number    = {8},
  publisher = {Company of Biologists},
  address   = {Cambridge},
  issn      = {0950-1991},
  doi       = {10.1242/dev.164301},
  pages     = {10},
  year      = {2018},
  abstract  = {Gene duplication is a major driver for the increase of biological complexity. The divergence of newly duplicated paralogs may allow novel functions to evolve, while maintaining the ancestral one. Alternatively, partitioning the ancestral function among paralogs may allow parts of that role to follow independent evolutionary trajectories. We studied the REDUCED COMPLEXITY (RCO) locus, which contains three paralogs that have evolved through two independent events of gene duplication, and which underlies repeated events of leaf shape evolution within the Brassicaceae. In particular, we took advantage of the presence of three potentially functional paralogs in Capsella to investigate the extent of functional divergence among them. We demonstrate that the RCO copies control growth in different areas of the leaf. Consequently, the copies that are retained active in the different Brassicaceae lineages contribute to define the leaf dissection pattern. Our results further illustrate how successive gene duplication events and subsequent functional divergence can increase trait evolvability by providing independent evolutionary trajectories to specialized functions that have an additive effect on a given trait.},
  language  = {en}
}
@article{JantzenWozniakKappeletal.2019,
  author    = {Jantzen, Friederike and Wozniak, Natalia Joanna and Kappel, Christian and Sicard, Adrien and Lenhard, Michael},
  title     = {A high‑throughput amplicon‑based method for estimating outcrossing rates},
  series = {Plant Methods},
  volume    = {15},
  journal   = {Plant Methods},
  number    = {47},
  publisher = {BioMed Central},
  address   = {London},
  issn      = {1746-4811},
  doi       = {10.1186/s13007-019-0433-9},
  pages     = {14},
  year      = {2019},
  abstract  = {Background: The outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on PCRgenotyping individual offspring of focal plants for multiple polymorphic markers is laborious and time-consuming. Results: We have developed an amplicon-based, high-throughput enabled method for estimating the outcrossing rate and have applied this to an example of scented versus non-scented Capsella (Shepherd's Purse) genotypes. Our results show that the method is able to robustly capture differences in outcrossing rates. They also highlight potential biases in the estimates resulting from differential haplotype sharing of the focal plants with the pollen-donor population at individual amplicons. Conclusions: This novel method for estimating outcrossing rates will allow determining this key population-genetic parameter with high-throughput across many genotypes in a population, enabling studies into the genetic determinants of successful pollinator attraction and outcrossing.},
  language  = {en}
}