@misc{WalterCollenburgJaptoketal.2016,
  author    = {Walter, Tim and Collenburg, Lena and Japtok, Lukasz and Kleuser, Burkhard and Schneider-Schaulies, Sibylle and M{\"u}ller, Nora and Becam, Jerome and Schubert-Unkmeir, Alexandra and Kong, Ji Na and Bieberich, Erhard and Seibel, J{\"u}rgen},
  title     = {Incorporation and visualization of azido-functionalized N-oleoyl serinol in Jurkat cells, mouse brain astrocytes, 3T3 fibroblasts and human brain microvascular endothelial cells},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-394960},
  pages     = {3},
  year      = {2016},
  abstract  = {The synthesis and biological evaluation of azido-N-oleoyl serinol is reported. It mimicks biofunctional lipid ceramides and has shown to be capable of click reactions for cell membrane imaging in Jurkat and human brain microvascular endothelial cells.},
  language  = {en}
}
@article{WalterCollenburgJaptoketal.2016,
  author    = {Walter, T. and Collenburg, Lena and Japtok, Lukasz and Kleuser, Burkhard and Schneider-Schaulies, Sibylle and Mueller, N. and Becam, Jerome and Schubert-Unkmeir, A. and Kong, J. N. and Bieberich, Erhard and Seibel, J.},
  title     = {Incorporation and visualization of azido-functionalized N-oleoyl serinol in Jurkat cells, mouse brain astrocytes, 3T3 fibroblasts and human brain microvascular endothelial cells},
  series = {Chemical communications},
  volume    = {52},
  journal   = {Chemical communications},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  issn      = {1359-7345},
  doi       = {10.1039/c6cc02879a},
  pages     = {8612 -- 8614},
  year      = {2016},
  abstract  = {The synthesis and biological evaluation of azido-N-oleoyl serinol is reported. It mimicks biofunctional lipid ceramides and has shown to be capable of click reactions for cell membrane imaging in Jurkat and human brain microvascular endothelial cells.},
  language  = {en}
}
@article{CollenburgWalterBurgertetal.2016,
  author    = {Collenburg, Lena and Walter, Tim and Burgert, Anne and Mueller, Nora and Seibel, Juergen and Japtok, Lukasz and Kleuser, Burkhard and Sauer, Markus and Schneider-Schaulies, Sibylle},
  title     = {A Functionalized Sphingolipid Analogue for Studying Redistribution during Activation in Living T Cells},
  series = {The journal of immunology},
  volume    = {196},
  journal   = {The journal of immunology},
  publisher = {American Assoc. of Immunologists},
  address   = {Bethesda},
  issn      = {0022-1767},
  doi       = {10.4049/jimmunol.1502447},
  pages     = {3951 -- 3962},
  year      = {2016},
  abstract  = {Sphingolipids are major components of the plasma membrane. In particular, ceramide serves as an essential building hub for complex sphingolipids, but also as an organizer of membrane domains segregating receptors and signalosomes. Sphingomyelin breakdown as a result of sphingomyelinase activation after ligation of a variety of receptors is the predominant source of ceramides released at the plasma membrane. This especially applies to T lymphocytes where formation of ceramide-enriched membrane microdomains modulates TCR signaling. Because ceramide release and redistribution occur very rapidly in response to receptor ligation, novel tools to further study these processes in living T cells are urgently needed. To meet this demand, we synthesized nontoxic, azido-functionalized ceramides allowing for bio-orthogonal click-reactions to fluorescently label incorporated ceramides, and thus investigate formation of ceramide-enriched domains. Azido-functionalized C-6-ceramides were incorporated into and localized within plasma membrane microdomains and proximal vesicles in T cells. They segregated into clusters after TCR, and especially CD28 ligation, indicating efficient sorting into plasma membrane domains associated with T cell activation; this was abolished upon sphingomyelinase inhibition. Importantly, T cell activation was not abrogated upon incorporation of the compound, which was efficiently excluded from the immune synapse center as has previously been seen in Ab-based studies using fixed cells. Therefore, the functionalized ceramides are novel, highly potent tools to study the subcellular redistribution of ceramides in the course of T cell activation. Moreover, they will certainly also be generally applicable to studies addressing rapid stimulation-mediated ceramide release in living cells.},
  language  = {en}
}