@article{WannerSeidlLampaHoehnetal.2016,
  author    = {Wanner, Manfred and Seidl-Lampa, Barbara and H{\"o}hn, Axel and Puppe, Daniel and Meisterfeld, Ralf and Sommer, Michael},
  title     = {Culture growth of testate amoebae under different silicon concentrations},
  series = {European journal of protistology},
  volume    = {56},
  journal   = {European journal of protistology},
  publisher = {Royal Society of Chemistry},
  address   = {Jena},
  organization    = {Veritas Collaboration},
  issn      = {0932-4739},
  doi       = {10.1016/j.ejop.2016.08.008},
  pages     = {171 -- 179},
  year      = {2016},
  abstract  = {Testate amoebae with self-secreted siliceous shell platelets ("idiosomes") play an important role in terrestrial silicon (Si) cycles. In this context, Si-dependent culture growth dynamics of idiosomic testate amoebae are of interest. Clonal cultures of idiosomic testate amoebae were analyzed under three different Si concentrations: low (50 mu mol L-1), moderate/site-specific (150 mu mol L-1) and high Si supply (500 mu mol L-1). Food (Saccharomyces cerevisiae) was provided in surplus. (i) Shell size of four different clones of idiosomic testate amoebae either decreased (Trinema galeata, Euglypha filifera cf.), increased (E. rotunda cf.), or did not change (E. rotunda) under the lowest Si concentration (50 mu mol Si L-1). (ii) Culture growth of idiosomic Euglypha rotunda was dependent on Si concentration. The more Si available in the culture medium, the earlier the entry into exponential growth phase. (iii) Culture growth of idiosomic Euglypha rotunda was dependent on origin of inoculum. Amoebae previously cultured under a moderate Si concentration revealed highest sustainability in consecutive cultures. Amoebae derived from cultures with high Si concentrations showed rapid culture growth which finished early in consecutive cultures. (iv) Si (diluted in the culture medium) was absorbed by amoebae and fixed in the amoeba shells resulting in decreased Si concentrations. (C) 2016 Elsevier GmbH. All rights reserved.},
  language  = {en}
}