@article{MajdaGronesSintornetal.2017,
  author    = {Majda, Mateusz and Grones, Peter and Sintorn, Ida-Maria and Vain, Thomas and Milani, Pascale and Krupinski, Pawel and Zagorska-Marek, Beata and Viotti, Corrado and Jonsson, Henrik and Mellerowicz, Ewa J. and Hamant, Olivier and Robert, Stephanie},
  title     = {Mechanochemical Polarization of Contiguous Cell Walls Shapes Plant Pavement Cells},
  series = {Developmental cell},
  volume    = {43},
  journal   = {Developmental cell},
  publisher = {Cell Press},
  address   = {Cambridge},
  issn      = {1534-5807},
  doi       = {10.1016/j.devcel.2017.10.017},
  pages     = {290 -- +},
  year      = {2017},
  abstract  = {The epidermis of aerial plant organs is thought to be limiting for growth, because it acts as a continuous load-bearing layer, resisting tension. Leaf epidermis contains jigsaw puzzle piece-shaped pavement cells whose shape has been proposed to be a result of subcellular variations in expansion rate that induce local buckling events. Paradoxically, such local compressive buckling should not occur given the tensile stresses across the epidermis. Using computational modeling, we show that the simplest scenario to explain pavement cell shapes within an epidermis under tension must involve mechanical wall heterogeneities across and along the anticlinal pavement cell walls between adjacent cells. Combining genetics, atomic force microscopy, and immunolabeling, we demonstrate that contiguous cell walls indeed exhibit hybrid mechanochemical properties. Such biochemical wall heterogeneities precede wall bending. Altogether, this provides a possible mechanism for the generation of complex plant cell shapes.},
  language  = {en}
}
@article{MaddockChilderstoneFryetal.2017,
  author    = {Maddock, Simon T. and Childerstone, Aaron and Fry, Bryan Grieg and Williams, David J. and Barlow, Axel and Wuester, Wolfgang},
  title     = {Multi-locus phylogeny and species delimitation of Australo-Papuan blacksnakes (Pseudechis Wagler, 1830: Elapidae: Serpentes)},
  series = {Molecular phylogenetics and evolution},
  volume    = {107},
  journal   = {Molecular phylogenetics and evolution},
  publisher = {Elsevier},
  address   = {San Diego},
  issn      = {1055-7903},
  doi       = {10.1016/j.ympev.2016.09.005},
  pages     = {48 -- 55},
  year      = {2017},
  abstract  = {Genetic analyses of Australasian organisms have resulted in the identification of extensive cryptic diversity across the continent. The venomous elapid snakes are among the best-studied organismal groups in this region, but many knowledge gaps persist: for instance, despite their iconic status, the species-level diversity among Australo-Papuan blacksnakes (Pseudechis) has remained poorly understood due to the existence of a group of cryptic species within the P. australis species complex, collectively termed "pygmy mulga snakes". Using two mitochondrial and three nuclear loci we assess species boundaries within the genus using Bayesian species delimitation methods and reconstruct their phylogenetic history using multispecies coalescent approaches. Our analyses support the recognition of 10 species, including all of the currently described pygmy mulga snakes and one undescribed species from the Northern Territory of Australia. Phylogenetic relationships within the genus are broadly consistent with previous work, with the recognition of three major groups, the viviparous red-bellied black snake P. porphyriacus forming the sister species to two clades consisting of ovoviviparous species.},
  language  = {en}
}
@misc{MachensBalazadehMuellerRoeberetal.2017,
  author    = {Machens, Fabian and Balazadeh, Salma and M{\"u}ller-R{\"o}ber, Bernd and Messerschmidt, Katrin},
  title     = {Synthetic Promoters and Transcription Factors for Heterologous Protein Expression in Saccharomyces cerevisiae},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-403804},
  pages     = {11},
  year      = {2017},
  abstract  = {Orthogonal systems for heterologous protein expression as well as for the engineering of synthetic gene regulatory circuits in hosts like Saccharomyces cerevisiae depend on synthetic transcription factors (synTFs) and corresponding cis-regulatory binding sites. We have constructed and characterized a set of synTFs based on either transcription activator-like effectors or CRISPR/Cas9, and corresponding small synthetic promoters (synPs) with minimal sequence identity to the host's endogenous promoters. The resulting collection of functional synTF/synP pairs confers very low background expression under uninduced conditions, while expression output upon induction of the various synTFs covers a wide range and reaches induction factors of up to 400. The broad spectrum of expression strengths that is achieved will be useful for various experimental setups, e.g., the transcriptional balancing of expression levels within heterologous pathways or the construction of artificial regulatory networks. Furthermore, our analyses reveal simple rules that enable the tuning of synTF expression output, thereby allowing easy modification of a given synTF/synP pair. This will make it easier for researchers to construct tailored transcriptional control systems.},
  language  = {en}
}
@article{MachensBalazadehMuellerRoeberetal.2017,
  author    = {Machens, Fabian and Balazadeh, Salma and M{\"u}ller-R{\"o}ber, Bernd and Messerschmidt, Katrin},
  title     = {Synthetic Promoters and Transcription Factors for Heterologous Protein Expression in Saccharomyces cerevisiae},
  series = {Frontiers in Bioengineering and Biotechnology},
  volume    = {5},
  journal   = {Frontiers in Bioengineering and Biotechnology},
  publisher = {Frontiers},
  address   = {Lausanne},
  issn      = {2296-4185},
  doi       = {10.3389/fbioe.2017.00063},
  pages     = {1 -- 11},
  year      = {2017},
  abstract  = {Orthogonal systems for heterologous protein expression as well as for the engineering of synthetic gene regulatory circuits in hosts like Saccharomyces cerevisiae depend on synthetic transcription factors (synTFs) and corresponding cis-regulatory binding sites. We have constructed and characterized a set of synTFs based on either transcription activator-like effectors or CRISPR/Cas9, and corresponding small synthetic promoters (synPs) with minimal sequence identity to the host's endogenous promoters. The resulting collection of functional synTF/synP pairs confers very low background expression under uninduced conditions, while expression output upon induction of the various synTFs covers a wide range and reaches induction factors of up to 400. The broad spectrum of expression strengths that is achieved will be useful for various experimental setups, e.g., the transcriptional balancing of expression levels within heterologous pathways or the construction of artificial regulatory networks. Furthermore, our analyses reveal simple rules that enable the tuning of synTF expression output, thereby allowing easy modification of a given synTF/synP pair. This will make it easier for researchers to construct tailored transcriptional control systems.},
  language  = {en}
}
@article{MaassDaphiLehmannetal.2017,
  author    = {Maass, Stefanie and Daphi, Daniel and Lehmann, Anika and Rillig, Matthias C.},
  title     = {Transport of microplastics by two collembolan species},
  series = {Environmental pollution},
  volume    = {225},
  journal   = {Environmental pollution},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {0269-7491},
  doi       = {10.1016/j.envpol.2017.03.009},
  pages     = {456 -- 459},
  year      = {2017},
  abstract  = {Plastics, despite their great benefits, have become a ubiquitous environmental pollutant, with micro-plastic particles having come into focus most recently. Microplastic effects have been intensely studied in aquatic, especially marine systems; however, there is lack of studies focusing on effects on soil and its biota. A basic question is if and how surface-deposited microplastic particles are transported into the soil. We here wished to test if soil microarthropods, using Collembola, can transport these particles over distances of centimeters within days in a highly controlled experimental set-up. We conducted a fully factorial experiment with two collembolan species of differing body size, Folsomia candida and Proisotoma minuta, in combination with urea-formaldehyde particles of two different particle sizes. We observed significant differences between the species concerning the distance the particles were transported. F. candida was able to transport larger particles further and faster than P. minuta. Using video, we observed F candida interacting with urea-formaldehyde particles and polyethylene terephthalate fibers, showing translocation of both material types. Our data clearly show that microplastic particles can be moved and distributed by soil microarthropods. Although we did not observe feeding, it is possible that microarthropods contribute to the accumulation of microplastics in the soil food web. (C) 2017 Elsevier Ltd. All rights reserved.},
  language  = {en}
}
@article{LuetkecosmannWarsinkeTschoepeetal.2017,
  author    = {L{\"u}tkecosmann, Steffi and Warsinke, Axel and Tsch{\"o}pe, Winfried and Eichler, R{\"u}diger and Hanack, Katja},
  title     = {A novel monoclonal antibody suitable for the detection of leukotriene B4},
  series = {Biochemical and biophysical research communications},
  volume    = {482},
  journal   = {Biochemical and biophysical research communications},
  number    = {4},
  publisher = {Elsevier},
  address   = {San Diego},
  issn      = {0006-291X},
  doi       = {10.1016/j.bbrc.2016.11.157},
  pages     = {1054 -- 1059},
  year      = {2017},
  abstract  = {Leukotriene B4 as an inflammatory mediator is an important biomarker for different respiratory diseases like asthma, chronic obstructive pulmonary disease or cystic lung fibrosis. Therefore the detection of LTB4 is helpful in the diagnosis of these pulmonary diseases. However, until now its determination in exhaled breath condensates suffers from problems of accuracy. Reasons for that could be improper sample collection and preparation methods of condensates and the lack of consistently assay specificity and reproducibility of the used immunoassay detection system. In this study we describe the development and the characterization of a specific monoclonal antibody (S27BC6) against LTB4, its use as molecular recognition element for the development of an enzyme-linked immunoassay to detect LTB4 and discuss possible future diagnostic applications.},
  language  = {en}
}
@article{LaemkeBaeurle2017,
  author    = {L{\"a}mke, J{\"o}rn and B{\"a}urle, Isabel},
  title     = {Epigenetic and chromatin-based mechanisms in environmental stress adaptation and stress memory in plants},
  series = {Genome biology : biology for the post-genomic era},
  volume    = {18},
  journal   = {Genome biology : biology for the post-genomic era},
  publisher = {BioMed Central},
  address   = {London},
  issn      = {1474-760X},
  doi       = {10.1186/s13059-017-1263-6},
  pages     = {8685 -- 8693},
  year      = {2017},
  abstract  = {Plants frequently have to weather both biotic and abiotic stressors, and have evolved sophisticated adaptation and defense mechanisms. In recent years, chromatin modifications, nucleosome positioning, and DNA methylation have been recognized as important components in these adaptations. Given their potential epigenetic nature, such modifications may provide a mechanistic basis for a stress memory, enabling plants to respond more efficiently to recurring stress or even to prepare their offspring for potential future assaults. In this review, we discuss both the involvement of chromatin in stress responses and the current evidence on somatic, intergenerational, and transgenerational stress memory.},
  language  = {en}
}
@misc{LucknerDunsingChiantiaetal.2017,
  author    = {Luckner, Madlen and Dunsing, Valentin and Chiantia, Salvatore and Herrmann, Andreas},
  title     = {Influenza virus vRNPs: quantitative investigations via fluorescence cross-correlation spectroscopy},
  series = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  volume    = {46},
  journal   = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  publisher = {Springer},
  address   = {New York},
  issn      = {0175-7571},
  pages     = {S368 -- S368},
  year      = {2017},
  language  = {en}
}
@article{LoveFeuersteinWolffetal.2017,
  author    = {Love, John A. and Feuerstein, Markus and Wolff, Christian Michael and Facchetti, Antonio and Neher, Dieter},
  title     = {Lead Halide Perovskites as Charge Generation Layers for Electron Mobility Measurement in Organic Semiconductors},
  series = {ACS applied materials \& interfaces},
  volume    = {9},
  journal   = {ACS applied materials \& interfaces},
  publisher = {American Chemical Society},
  address   = {Washington},
  issn      = {1944-8244},
  doi       = {10.1021/acsami.7b10361},
  pages     = {42011 -- 42019},
  year      = {2017},
  abstract  = {Hybrid lead halide perovskites are introduced as charge generation layers (CGLs) for the accurate determination of electron mobilities in thin organic semiconductors. Such hybrid perovskites have become a widely studied photovoltaic material in their own right, for their high efficiencies, ease of processing from solution, strong absorption, and efficient photogeneration of charge. Time-of-flight (ToF) measurements on bilayer samples consisting of the perovskite CGL and an organic semiconductor layer of different thickness are shown to be determined by the carrier motion through the organic material, consistent with the much higher charge carrier mobility in the perovskite. Together with the efficient photon-to-electron conversion in the perovskite, this high mobility imbalance enables electron-only mobility measurement on relatively thin application-relevant organic films, which would not be possible with traditional ToF measurements. This architecture enables electron-selective mobility measurements in single components as well as bulk-heterojunction films as demonstrated in the prototypical polymer/fullerene blends. To further demonstrate the potential of this approach, electron mobilities were measured as a function of electric field and temperature in an only 127 nm thick layer of a prototypical electron-transporting perylene diimide-based polymer, and found to be consistent with an exponential trap distribution of ca. 60 meV. Our study furthermore highlights the importance of high mobility charge transporting layers when designing perovskite solar cells.},
  language  = {en}
}
@phdthesis{Loiacono2017,
  author    = {Loiacono, Filomena Vanessa},
  title     = {Transfer of chloroplast RNA editing events between species},
  school      = {Universit{\"a}t Potsdam},
  pages     = {155},
  year      = {2017},
  language  = {en}
}
@article{LischkeMehnerHiltetal.2017,
  author    = {Lischke, Betty and Mehner, Thomas and Hilt, Sabine and Attermeyer, Katrin and Brauns, Mario and Brothers, Soren M. and Grossart, Hans-Peter and Koehler, Jan and Scharnweber, Inga Kristin and Gaedke, Ursula},
  title     = {Benthic carbon is inefficiently transferred in the food webs of two eutrophic shallow lakes},
  series = {Freshwater biology},
  volume    = {62},
  journal   = {Freshwater biology},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0046-5070},
  doi       = {10.1111/fwb.12979},
  pages     = {1693 -- 1706},
  year      = {2017},
  abstract  = {The sum of benthic autotrophic and bacterial production often exceeds the sum of pelagic autotrophic and bacterial production, and hence may contribute substantially to whole-lake carbon fluxes, especially in shallow lakes. Furthermore, both benthic and pelagic autotrophic and bacterial production are highly edible and of sufficient nutritional quality for animal consumers. We thus hypothesised that pelagic and benthic transfer efficiencies (ratios of production at adjacent trophic levels) in shallow lakes should be similar. We performed whole ecosystem studies in two shallow lakes (3.5ha, mean depth 2m), one with and one without submerged macrophytes, and quantified pelagic and benthic biomass, production and transfer efficiencies for bacteria, phytoplankton, epipelon, epiphyton, macrophytes, zooplankton, macrozoobenthos and fish. We expected higher transfer efficiencies in the lake with macrophytes, because these provide shelter and food for macrozoobenthos and may thus enable a more efficient conversion of basal production to consumer production. In both lakes, the majority of the whole-lake autotrophic and bacterial production was provided by benthic organisms, but whole-lake primary consumer production mostly relied on pelagic autotrophic and bacterial production. Consequently, transfer efficiency of benthic autotrophic and bacterial production to macrozoobenthos production was an order of magnitude lower than the transfer efficiency of pelagic autotrophic and bacterial production to rotifer and crustacean production. Between-lake differences in transfer efficiencies were minor. We discuss several aspects potentially causing the unexpectedly low benthic transfer efficiencies, such as the food quality of producers, pelagic-benthic links, oxygen concentrations in the deeper lake areas and additional unaccounted consumer production by pelagic and benthic protozoa and meiobenthos at intermediate or top trophic levels. None of these processes convincingly explain the large differences between benthic and pelagic transfer efficiencies. Our data indicate that shallow eutrophic lakes, even with a major share of autotrophic and bacterial production in the benthic zone, can function as pelagic systems with respect to primary consumer production. We suggest that the benthic autotrophic production was mostly transferred to benthic bacterial production, which remained in the sediments, potentially cycling internally in a similar way to what has previously been described for the microbial loop in pelagic habitats. Understanding the energetics of whole-lake food webs, including the fate of the substantial benthic bacterial production, which is either mineralised at the sediment surface or permanently buried, has important implications for regional and global carbon cycling.},
  language  = {en}
}
@article{LibradoGambaGaunitzetal.2017,
  author    = {Librado, Pablo and Gamba, Cristina and Gaunitz, Charleen and Sarkissian, Clio Der and Pruvost, Melanie and Albrechtsen, Anders and Fages, Antoine and Khan, Naveed and Schubert, Mikkel and Jagannathan, Vidhya and Serres-Armero, Aitor and Kuderna, Lukas F. K. and Povolotskaya, Inna S. and Seguin-Orlando, Andaine and Lepetz, Sebastien and Neuditschko, Markus and Theves, Catherine and Alquraishi, Saleh A. and Alfarhan, Ahmed H. and Al-Rasheid, Khaled A. S. and Rieder, Stefan and Samashev, Zainolla and Francfort, Henri-Paul and Benecke, Norbert and Hofreiter, Michael and Ludwig, Arne and Keyser, Christine and Marques-Bonet, Tomas and Ludes, Bertrand and Crubezy, Eric and Leeb, Tosso and Willerslev, Eske and Orlando, Ludovic},
  title     = {Ancient genomic changes associated with domestication of the horse},
  series = {Science},
  volume    = {356},
  journal   = {Science},
  publisher = {American Assoc. for the Advancement of Science},
  address   = {Washington},
  issn      = {0036-8075},
  doi       = {10.1126/science.aam5298},
  pages     = {442 -- 445},
  year      = {2017},
  abstract  = {The genomic changes underlying both early and late stages of horse domestication remain largely unknown. We examined the genomes of 14 early domestic horses from the Bronze and Iron Ages, dating to between similar to 4.1 and 2.3 thousand years before present. We find early domestication selection patterns supporting the neural crest hypothesis, which provides a unified developmental origin for common domestic traits. Within the past 2.3 thousand years, horses lost genetic diversity and archaic DNA tracts introgressed from a now-extinct lineage. They accumulated deleterious mutations later than expected under the cost-of-domestication hypothesis, probably because of breeding from limited numbers of stallions. We also reveal that Iron Age Scythian steppe nomads implemented breeding strategies involving no detectable inbreeding and selection for coat-color variation and robust forelimbs.},
  language  = {en}
}
@article{LiXuWangetal.2017,
  author    = {Li, Zhengdong and Xu, Xun and Wang, Weiwei and Kratz, Karl and Sun, Xianlei and Zou, Jie and Deng, Zijun and Jung, Friedrich Wilhelm and Gossen, Manfred and Ma, Nan and Lendlein, Andreas},
  title     = {Modulation of the mesenchymal stem cell migration capacity via preconditioning with topographic microstructure},
  series = {Clinical hemorheology and microcirculation : blood flow and vessels},
  volume    = {67},
  journal   = {Clinical hemorheology and microcirculation : blood flow and vessels},
  publisher = {IOS Press},
  address   = {Amsterdam},
  issn      = {1386-0291},
  doi       = {10.3233/CH-179208},
  pages     = {267 -- 278},
  year      = {2017},
  abstract  = {Controlling mesenchymal stem cells (MSCs) behavior is necessary to fully exploit their therapeutic potential. Various approaches are employed to effectively influence the migration capacity of MSCs. Here, topographic microstructures with different microscale roughness were created on polystyrene (PS) culture vessel surfaces as a feasible physical preconditioning strategy to modulate MSC migration. By analyzing trajectories of cells migrating after reseeding, we demonstrated that the mobilization velocity of human adipose derived mesenchymal stem cells (hADSCs) could be promoted by and persisted after brief preconditioning with the appropriate microtopography. Moreover, the elevated activation levels of focal adhesion kinase (FAK) and mitogen-activated protein kinase (MAPK) in hADSCs were also observed during and after the preconditioning process. These findings underline the potential enhancement of in vivo therapeutic efficacy in regenerative medicine via transplantation of topographic microstructure preconditioned stem cells.},
  language  = {en}
}
@misc{Lenhard2017,
  author    = {Lenhard, Michael},
  title     = {Plant Development: Keeping on the Straight and Narrow and Flat},
  series = {Current biology},
  volume    = {27},
  journal   = {Current biology},
  publisher = {Cell Press},
  address   = {Cambridge},
  issn      = {0960-9822},
  doi       = {10.1016/j.cub.2017.10.030},
  pages     = {R1277 -- R1280},
  year      = {2017},
  language  = {en}
}
@article{LeimkuehlerMendel2017,
  author    = {Leimk{\"u}hler, Silke and Mendel, Ralf-Rainer},
  title     = {Molybdenum Cofactor Biosynthesis},
  series = {Molybdenum and tungsten enzymes: biochemistry},
  volume    = {5},
  journal   = {Molybdenum and tungsten enzymes: biochemistry},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  isbn      = {978-1-78262-391-5},
  doi       = {10.1039/9781782623915},
  pages     = {100 -- 116},
  year      = {2017},
  abstract  = {The biosynthesis of the molybdenum cofactor (Moco) is highly conserved among all kingdoms of life. In all molybdoenzymes with the exception of nitrogenase, the molybdenum atom is coordinated to a dithiolene group present in the pterin-based 6-alkyl side chain of molybdopterin (MPT). In general, the biosynthesis of Moco can be divided into three steps in eukaryotes, and four steps in bacteria and archaea: (i) the starting point is the formation of the cyclic pyranopterin monophosphate (cPMP) from 5′GTP, (ii) in the second step the two sulfur molecules are inserted into cPMP leading to the formation of MPT, (iii) in the third step the molybdenum atom is inserted into molybdopterin to form Moco and (iv) additional modification of Moco occurs in bacteria and archaea with the attachment of a nucleotide (CMP or GMP) to the phosphate group of MPT, forming the dinucleotide variants of Moco. This review will focus on the biosynthesis of Moco in bacteria, humans and plants.},
  language  = {en}
}
@article{LeimkuehlerLemaireIobbiNivol2017,
  author    = {Leimk{\"u}hler, Silke and Lemaire, Olivier N. and Iobbi-Nivol, Chantal},
  title     = {Bacterial Molybdoenzymes},
  series = {Molybdenum and tungsten enzymes : biochemistry},
  volume    = {5},
  journal   = {Molybdenum and tungsten enzymes : biochemistry},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  isbn      = {978-1-78262-391-5},
  doi       = {10.1039/9781782623915-00117},
  pages     = {117 -- 142},
  year      = {2017},
  abstract  = {The biogenesis of molybdoenzymes is a cytoplasmic event requiring both the folded apoenzymes and the matured molybdenum cofactor. The structure and the complexity of the molybdenum cofactor varies in each molybdoenzyme family and consequently different accessory proteins are required for the maturation of the respective enzymes. Thus, for enzymes of both the DMSO reductase and xanthine oxidase families, specific chaperones exist which are dedicated to increase the stability and the folding of specific members of each family. In this review, we describe the role of these chaperones for molybdoenzyme maturation. We present a model which describes step by step the mechanism of the maturation of representative molybdoenzymes from each family.},
  language  = {en}
}
@misc{LeimkuehlerBuehningBeilschmidt2017,
  author    = {Leimk{\"u}hler, Silke and B{\"u}hning, Martin and Beilschmidt, Lena},
  title     = {Shared sulfur mobilization routes for tRNA thiolation and molybdenum cofactor biosynthesis in prokaryotes and eukaryotes},
  series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe},
  journal   = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe},
  number    = {1015},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-47501},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-475011},
  pages     = {22},
  year      = {2017},
  abstract  = {Modifications of transfer RNA (tRNA) have been shown to play critical roles in the biogenesis, metabolism, structural stability and function of RNA molecules, and the specific modifications of nucleobases with sulfur atoms in tRNA are present in pro- and eukaryotes. Here, especially the thiomodifications xm(5)s(2)U at the wobble position 34 in tRNAs for Lys, Gln and Glu, were suggested to have an important role during the translation process by ensuring accurate deciphering of the genetic code and by stabilization of the tRNA structure. The trafficking and delivery of sulfur nucleosides is a complex process carried out by sulfur relay systems involving numerous proteins, which not only deliver sulfur to the specific tRNAs but also to other sulfur-containing molecules including iron-sulfur clusters, thiamin, biotin, lipoic acid and molybdopterin (MPT). Among the biosynthesis of these sulfur-containing molecules, the biosynthesis of the molybdenum cofactor (Moco) and the synthesis of thio-modified tRNAs in particular show a surprising link by sharing protein components for sulfur mobilization in pro- and eukaryotes.},
  language  = {en}
}
@misc{LeimkuehlerBuehningBeilschmidt2017,
  author    = {Leimk{\"u}hler, Silke and B{\"u}hning, Martin and Beilschmidt, Lena},
  title     = {Shared sulfur mobilization routes for tRNA thiolation and molybdenum cofactor biosynthesis in prokaryotes and eukaryotes},
  series = {Biomolecules},
  volume    = {7},
  journal   = {Biomolecules},
  number    = {1},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2218-273X},
  doi       = {10.3390/biom7010005},
  pages     = {20},
  year      = {2017},
  abstract  = {Modifications of transfer RNA (tRNA) have been shown to play critical roles in the biogenesis, metabolism, structural stability and function of RNA molecules, and the specific modifications of nucleobases with sulfur atoms in tRNA are present in pro- and eukaryotes. Here, especially the thiomodifications xm(5)s(2)U at the wobble position 34 in tRNAs for Lys, Gln and Glu, were suggested to have an important role during the translation process by ensuring accurate deciphering of the genetic code and by stabilization of the tRNA structure. The trafficking and delivery of sulfur nucleosides is a complex process carried out by sulfur relay systems involving numerous proteins, which not only deliver sulfur to the specific tRNAs but also to other sulfur-containing molecules including iron-sulfur clusters, thiamin, biotin, lipoic acid and molybdopterin (MPT). Among the biosynthesis of these sulfur-containing molecules, the biosynthesis of the molybdenum cofactor (Moco) and the synthesis of thio-modified tRNAs in particular show a surprising link by sharing protein components for sulfur mobilization in pro- and eukaryotes.},
  language  = {en}
}
@misc{Leimkuehler2017,
  author    = {Leimk{\"u}hler, Silke},
  title     = {Shared function and moonlighting proteins in molybdenum cofactor biosynthesis},
  series = {Biological chemistry},
  volume    = {398},
  journal   = {Biological chemistry},
  publisher = {De Gruyter},
  address   = {Berlin},
  issn      = {1431-6730},
  doi       = {10.1515/hsz-2017-0110},
  pages     = {1009 -- 1026},
  year      = {2017},
  abstract  = {The biosynthesis of the molybdenum cofactor (Moco) is a highly conserved pathway in bacteria, archaea and eukaryotes. The molybdenum atom in Moco-containing enzymes is coordinated to the dithiolene group of a tricyclic pyranopterin monophosphate cofactor. The biosynthesis of Moco can be divided into three conserved steps, with a fourth present only in bacteria and archaea: (1) formation of cyclic pyranopterin monophosphate, (2) formation of molybdopterin (MPT), (3) insertion of molybdenum into MPT to form Mo-MPT, and (4) additional modification of Mo-MPT in bacteria with the attachment of a GMP or CMP nucleotide, forming the dinucleotide variants of Moco. While the proteins involved in the catalytic reaction of each step of Moco biosynthesis are highly conserved among the Phyla, a surprising link to other cellular pathways has been identified by recent discoveries. In particular, the pathways for FeS cluster assembly and thio-modifications of tRNA are connected to Moco biosynthesis by sharing the same protein components. Further, proteins involved in Moco biosynthesis are not only shared with other pathways, but additionally have moonlighting roles. This review gives an overview of Moco biosynthesis in bacteria and humans and highlights the shared function and moonlighting roles of the participating proteins.},
  language  = {en}
}
@article{LehmannFlorisWoiteketal.2017,
  author    = {Lehmann, Andreas and Floris, Jo{\"e}l and Woitek, Ulrich and Ruehli, Frank J. and Staub, Kaspar},
  title     = {Temporal trends, regional variation and socio-economic differences in height, BMI and body proportions among German conscripts, 1956-2010},
  series = {Public Health Nutrition},
  volume    = {20},
  journal   = {Public Health Nutrition},
  number    = {3},
  publisher = {Cambridge Univ. Press},
  address   = {Cambridge},
  issn      = {1368-9800},
  doi       = {10.1017/S1368980016002408},
  pages     = {391 -- 403},
  year      = {2017},
  abstract  = {Objective: We analyse temporal trends and regional variation among the most recent available anthropometric data from German conscription in the years 2008-2010 and their historical contextualization since 1956. Design/setting/subjects: The overall sample included German conscripts (N 13 857 313) from 1956 to 2010. Results: German conscripts changed from growing in height to growing in breadth. Over the analysed 54 years, average height of 19-year-old conscripts increased by 6.5 cm from 173.5 cm in 1956 (birth year 1937) to 180.0 cm in 2010 (birth year 1991). This increase plateaued since the 1990s (1970s birth years). The increase in average weight, however, did not lessen during the last two decades but increased in two steps: at the end of the 1980s and after 1999. The weight and BMI distributions became increasingly right-skewed, the prevalence of overweight and obesity increased from 11.6 \% and 2.1 \% in 1984 to 19.9 \% and 8.5 \% in 2010, respectively. The north-south gradient in height (north = taller) persisted during our observations. Height and weight of conscripts from East Germany matched the German average between the early 1990s and 2009. Between the 1980s and the early 1990s, the average chest circumference increased, the average difference between chest circumference when inhaling and exhaling decreased, as did leg length relative to trunk length. Conclusions: Measuring anthropometric data for military conscripts yielded year-by-year monitoring of the health status of young men at a proscribed age. Such findings contribute to a more precise identification of groups at risk and thus help with further studies and to target interventions.},
  language  = {en}
}
@article{LecourieuxKappelPierietal.2017,
  author    = {Lecourieux, Fatma and Kappel, Christian and Pieri, Philippe and Charon, Justine and Pillet, Jeremy and Hilbert, Ghislaine and Renaud, Christel and Gomes, Eric and Delrot, Serge and Lecourieux, David},
  title     = {Dissecting the Biochemical and Transcriptomic Effects of a Locally Applied Heat Treatment on Developing Cabernet Sauvignon Grape Berries},
  series = {Frontiers in plant science},
  volume    = {8},
  journal   = {Frontiers in plant science},
  publisher = {Frontiers Research Foundation},
  address   = {Lausanne},
  issn      = {1664-462X},
  doi       = {10.3389/fpls.2017.00053},
  pages     = {23},
  year      = {2017},
  abstract  = {Reproductive development of grapevine and berry composition are both strongly influenced by temperature. To date, the molecular mechanisms involved in grapevine berries response to high temperatures are poorly understood. Unlike recent data that addressed the effects on berry development of elevated temperatures applied at the whole plant level, the present work particularly focuses on the fruit responses triggered by direct exposure to heat treatment (HT). In the context of climate change, this work focusing on temperature effect at the microclimate level is of particular interest as it can help to better understand the consequences of leaf removal (a common viticultural practice) on berry development. HT (+8 degrees C) was locally applied to clusters from Cabernet Sauvignon fruiting cuttings at three different developmental stages (middle green, veraison and middle ripening). Samples were collected 1, 7, and 14 days after treatment and used for metabolic and transcriptomic analyses. The results showed dramatic and specific biochemical and transcriptomic changes in heat exposed berries, depending on the developmental stage and the stress duration. When applied at the herbaceous stage, HT delayed the onset of veraison. Heating also strongly altered the berry concentration of amino acids and organic acids (e.g., phenylalanine, raminobutyric acid and malate) and decreased the anthocyanin content at maturity. These physiological alterations could be partly explained by the deep remodeling of transcriptome in heated berries. More than 7000 genes were deregulated in at least one of the nine experimental conditions. The most affected processes belong to the categories "stress responses," protein metabolism" and "secondary metabolism," highlighting the intrinsic capacity of grape berries to perceive HT and to build adaptive responses. Additionally, important changes in processes related to "transport," "hormone" and "cell wall" might contribute to the postponing of veraison. Finally, opposite effects depending on heating duration were observed for genes encoding enzymes of the general phenylpropanoid pathway, suggesting that the HI induced decrease in anthocyanin content may result from a combination of transcript abundance and product degradation.},
  language  = {en}
}
@misc{LauxKniggeWengeretal.2017,
  author    = {Laux, Eva-Maria and Knigge, Xenia and Wenger, C. and Bier, Frank Fabian and H{\"o}lzel, Ralph},
  title     = {AC electrokinetic manipulation of nanoparticles and molecules},
  series = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  volume    = {46},
  journal   = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  publisher = {Springer},
  address   = {New York},
  issn      = {0175-7571},
  pages     = {S189 -- S189},
  year      = {2017},
  language  = {en}
}
@misc{LauxGibbonsErmilovaetal.2017,
  author    = {Laux, Eva-Maria and Gibbons, J. and Ermilova, Elena and Bier, Frank Fabian and H{\"o}lzel, Ralph},
  title     = {Broadband dielectric spectroscopy of bovine serum albumin in the GHz range},
  series = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  volume    = {46},
  journal   = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  publisher = {Springer},
  address   = {New York},
  issn      = {0175-7571},
  pages     = {S347 -- S347},
  year      = {2017},
  language  = {en}
}
@misc{LauxDocoslisWengeretal.2017,
  author    = {Laux, Eva-Maria and Docoslis, A. and Wenger, C. and Bier, Frank Fabian and H{\"o}lzel, Ralph},
  title     = {Combination of dielectrophoresis and SERS for bacteria detection and characterization},
  series = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  volume    = {46},
  journal   = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  publisher = {Springer},
  address   = {New York},
  issn      = {0175-7571},
  pages     = {S331 -- S331},
  year      = {2017},
  language  = {en}
}
@article{LauHupferGrossart2017,
  author    = {Lau, Maximilian P. and Hupfer, Michael and Grossart, Hans-Peter},
  title     = {Reduction-oxidation cycles of organic matter increase bacterial activity in the pelagic oxycline},
  series = {Environmental microbiology reports},
  volume    = {9},
  journal   = {Environmental microbiology reports},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {1758-2229},
  doi       = {10.1111/1758-2229.12526},
  pages     = {257 -- 267},
  year      = {2017},
  abstract  = {Dissolved organic matter (DOM) in aquatic ecosystems contains redox-active moieties, which are prone to oxidation and reduction reactions. Oxidized moieties feature reduction potentials E-h, so that the moieties may be used as terminal electron acceptors (TEAs) in microbial respiration with a thermodynamic energy yield between nitrate and sulfate reduction. Here, we study the response of pelagic freshwater bacteria to exposure to native DOM with varying availabilities of oxidized moieties and hence redox state. Our results show that the prevalence of oxidized DOM favors microbial production and growth in anoxic waters. Reduced DOM in stratified lakes may be oxidized when fluctuations of the oxycline expose DOM in previously anoxic water to epilimnetic oxygen. The resulting oxidized DOM may be rapidly used as TEAs in microbial respiration during subsequent periods of anoxia. We further investigate if the prevalence of these organic electron sinks in anaerobic incubations can induce changes in the microbial community. Our results reveal that DOM traversing transient redox interfaces selects for species that profit from such spatially confined and cyclically restored TEA reservoirs.},
  language  = {en}
}
@article{LampeiMetzTielboerger2017,
  author    = {Lampei, Christian and Metz, Johannes and Tielboerger, Katja},
  title     = {Clinal population divergence in an adaptive parental environmental effect that adjusts seed banking},
  series = {New phytologist : international journal of plant science},
  volume    = {214},
  journal   = {New phytologist : international journal of plant science},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0028-646X},
  doi       = {10.1111/nph.14436},
  pages     = {1230 -- 1244},
  year      = {2017},
  abstract  = {Bet-hedging via between-year seed dormancy is a costly strategy for plants in unpredictable environments. Theoretically, fitness costs can be reduced through a parental environmental effect when the environment is partly predictable. We tested whether populations from environments that differ in predictability diverged in parental effects on seed dormancy. Common garden-produced seeds of the two annual plant species Biscutella didyma and Bromus fasciculatus collected along an aridity gradient were grown under 12 irrigation treatments. Offspring germination was evaluated and related to environmental correlations between generations and their fitness consequences at the four study sites. One species exhibited strong seed dormancy that increased with unpredictability in seasonal precipitation. The parental effect on seed dormancy also increased proportionally with the environmental correlation between precipitation in the parental season and seedling density in the following season; this correlation increased from mesic to arid environments. Because fitness was negatively related to density, this parental effect may be adaptive. However, the lack of dormancy in the second species indicates that bet-hedging is not the only strategy for annual plants in arid environments. Our results provide the first evidence for clinal variation in the relative strength of parental effects along environmental gradients.},
  language  = {en}
}
@article{LahLoeberHsiangetal.2017,
  author    = {Lah, Ljerka and L{\"o}ber, Ulrike and Hsiang, Tom and Hartmann, Stefanie},
  title     = {A genomic comparison of putative pathogenicity-related gene families in five members of the Ophiostomatales with different lifestyles},
  series = {Fungal biology},
  volume    = {121},
  journal   = {Fungal biology},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {1878-6146},
  doi       = {10.1016/j.funbio.2016.12.002},
  pages     = {234 -- 252},
  year      = {2017},
  abstract  = {Ophiostomatoid fungi are vectored by their bark-beetle associates and colonize different host tree species. To survive and proliferate in the host, they have evolved mechanisms for detoxification and elimination of host defence compounds, efficient nutrient sequestration, and, in pathogenic species, virulence towards plants. Here, we assembled a draft genome of the spruce pathogen Ophiostoma bicolor. For our comparative and phylogenetic analyses, we mined the genomes of closely related species (Ophiostoma piceae, Ophiostoma ulmi, Ophiostoma novo-ulmi, and Grosmannia clavigera). Our aim was to acquire a genomic and evolutionary perspective of gene families important in host colonization. Genome comparisons showed that both the nuclear and mitochondrial genomes in our assembly were largely complete. Our O. bicolor 25.3 Mbp draft genome had 10 018 predicted genes, 6041 proteins with gene ontology (GO) annotation, 269 carbohydrate-active enzymes (CAZymes), 559 peptidases and inhibitors, and 1373 genes likely involved in pathogen-host interactions. Phylogenetic analyses of selected protein families revealed core sets of cytochrome P450 genes, ABC transporters and backbone genes involved in secondary metabolite (SM) biosynthesis (polyketide synthases (PKS) and non-ribosomal synthases), and species-specific gene losses and duplications. Phylogenetic analyses of protein families of interest provided insight into evolutionary adaptations to host biochemistry in ophiostomatoid fungi.},
  language  = {en}
}
@article{LachmannMaberlySpijkerman2017,
  author    = {Lachmann, Sabrina C. and Maberly, Stephen C. and Spijkerman, Elly},
  title     = {Species-specific influence of P-i-status on inorganic carbon acquisition in microalgae (Chlorophyceae)},
  series = {Botany},
  volume    = {95},
  journal   = {Botany},
  publisher = {NRC Research Press},
  address   = {Ottawa},
  issn      = {1916-2790},
  doi       = {10.1139/cjb-2017-0082},
  pages     = {943 -- 952},
  year      = {2017},
  abstract  = {Inorganic phosphorus (P-i) is often the primary limiting nutrient in freshwater ecosystems. Since P(i-)limitation affects energy transduction, and inorganic carbon (C-i) acquisition can be energy demanding, C(i-)acquisition strategies were compared in four species of green algae grown under P-i-replete and P-i-limited conditions predominantly at low and partly at high CO2. Although P-i-limitation was evident by the 10-fold higher cellular C:P ratio and enhanced phosphatase activity, it only decreased C-i-acquisition to a small extent. Nonetheless, the effects of Pi-limitation on both CO2 and HCO3- acquisition were demonstrated. Decreased CO2 acquisition under conditions of Pi limitation was mainly visible in the maximum uptake rate (V-max) and, for the neutrophile Scenedesmus vacuolatus, in the affinity for CO2 acquisition. Discrimination against C-13 was higher under P-i-limited, high CO2 conditions, compared with P-i-replete, highCO(2) conditions, in Chlamydomonas acidophila and S. vacuolatus. In the pH-drift experiments, HCO3- acquisition was reduced in P-i-limited C. reinhardtii. In general, energy demanding bicarbonate uptake was indicated by the less strong discrimination against (13)Cunder lowCO(2) conditions in the neutrophiles (HCO3- users), separating them from the acidophilic or acidotolerant species (CO2 users). The high variability of the influence of Pi supply among different green algal species is linked to their species-specific C(i-)acquisition strategies.},
  language  = {en}
}
@phdthesis{Lachmann2017,
  author    = {Lachmann, Sabrina C.},
  title     = {Ecophysiology matters: Inorganic carbon acquisition in green microalgae related to different nutrient conditions},
  school      = {Universit{\"a}t Potsdam},
  pages     = {178},
  year      = {2017},
  language  = {en}
}
@article{KurzeHeinkenFartmann2017,
  author    = {Kurze, Susanne and Heinken, Thilo and Fartmann, Thomas},
  title     = {Nitrogen enrichment of host plants has mostly beneficial effects on the life-history traits of nettle-feeding butterflies},
  series = {Acta oecologica : international journal of ecology},
  volume    = {85},
  journal   = {Acta oecologica : international journal of ecology},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1146-609X},
  doi       = {10.1016/j.actao.2017.11.005},
  pages     = {157 -- 164},
  year      = {2017},
  abstract  = {Butterflies rank among the most threatened animal groups throughout Europe. However, current population trends differ among species. The nettle-feeding butterflies Aglais io and Aglais urticae cope successfully with the anthropogenic land-use change. Both species are assumed to be pre-adapted to higher nitrogen contents in their host plant, stinging nettle (Urtica dioica). However, it is currently unknown, whether this pre-adaptation enables both Aglais species to cope successfully or even to benefit from the excessive nitrogen availabilities in nettles growing in modern farmlands. For this reason, this study focused on the response of both Aglais species to unfertilized nettles compared to nettles receiving 150 or 300 kg N ha(-1) yr(-1) (i.e., common fertilizer quantities of modern-day agriculture). Fertilized nettles were characterized by higher nitrogen concentrations and lower C:N ratios compared to the control group. In both Aglais species, the individuals feeding on fertilized nettles had higher survival rates, shorter larval periods and heavier pupae and, in A. urticae also longer forewings. All these trait shifts are beneficial for the individuals, lowering their risk to die before reproduction and increasing their reproductive potential. These responses agree with the well-accepted nitrogen-limitation hypothesis predicting a positive relationship between the nitrogen content of the diet and the performance of herbivorous insects. Furthermore, our findings suggest that the increasing abundance of both Aglais species may result not only from the increasing spread of nettles into the farmland but also from changes in their quality due to the eutrophication of the landscape during recent decades.},
  language  = {en}
}
@article{KurzeBareitherMetz2017,
  author    = {Kurze, Susanne and Bareither, Nils and Metz, Johannes},
  title     = {Phenology, roots and reproductive allocation, but not the LHS scheme, shape ecotypes along an aridity gradient},
  series = {Perspectives in plant ecology, evolution and systematics},
  volume    = {29},
  journal   = {Perspectives in plant ecology, evolution and systematics},
  publisher = {Elsevier},
  address   = {Jena},
  issn      = {1433-8319},
  doi       = {10.1016/j.ppees.2017.09.004},
  pages     = {20 -- 29},
  year      = {2017},
  abstract  = {This study tested systematically at two spatial scales for key traits shaping within-species ecotypic differentiation under increasing aridity. It assessed different plant strategy theories and considered potential implications for climate change. We studied the widespread Mediterranean grass Brachypodium hybridum. At large scale, we tested 14 populations along a steep natural aridity gradient (114-954 mm annual rainfall). At small scale, we tested the microclimatic contrast between plants originating from corresponding north (more mesic) and south (more arid) exposed hillslopes. Fifteen traits were measured in the greenhouse, including the popular traits of the LeafHeight- Seed scheme (SLA, plant height, seed mass), several traits on phenology, architecture, growth, fitness, and rarely measured root traits. Clear trait shifts indicated ecotypic differentiation along the large-scale gradient. Earlier phenology, higher reproductive allocation and reduced root investment characterized arid ecotypes. Surprisingly, no trait of the Leaf-Height-Seed scheme shifted with aridity and root responses were opposite to the theory of optimal resource partitioning. Trait differences between north and south exposures were small, often inconsistent between sites, and poorly matched the trends across the large-scale gradient. South exposures thus appeared unlikely to harbour distinct ecotypes better adapted to aridity. Our findings highlight ecotypes as a crucial way how species span environmental gradients, yet underpinning their restriction at small spatial scales. In combination, this possibly renders populations more vulnerable to climate change. We draw attention to specific, partly unexpected traits and pose the question whether the LeafHeight- Seed scheme has limited applicability for intraspecific investigations in drylands.},
  language  = {en}
}
@phdthesis{Kunstmann2017,
  author    = {Kunstmann, Ruth Sonja},
  title     = {Design of a high-affinity carbohydrate binding protein},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-403458},
  school      = {Universit{\"a}t Potsdam},
  pages     = {XI, 169},
  year      = {2017},
  abstract  = {Kohlenhydrat-Protein Interaktionen sind in der Natur weitverbreitet. Sie stellen die Grundlage f{\"u}r viele biologische Prozesse dar, wie zum Beispiel Immunantworten, Wundheilung und Infektionsprozesse von pathogenen Viren oder Bakterien mit einem Wirt wie dem Menschen. Neben der Infektion von Menschen k{\"o}nnen aber auch Bakterien selbst durch so genannte Bakteriophagen infiziert werden, welche f{\"u}r den Menschen ungef{\"a}hrlich sind. Diese Infektion involviert die spezifische Erkennung der pathogenen Bakterien, die Vermehrung der Bakteriophagen und schließlich die Abt{\"o}tung der Bakterien. Dabei k{\"o}nnen die Mechanismen der spezifischen Erkennung genutzt werden, pathogene Bakterien auf Lebensmitteln zu detektieren oder die Diagnose von Infektionen zu vereinfachen. Die spezifische Erkennung von Enteritis-erzeugenden Bakterien wie Escherichia coli, Salmonella spp. oder Shigella flexneri durch Bakteriophagen der Familie der Podoviridae erfolgt {\"u}ber die Bindung eines sogenannten tailspike proteins des Bakteriophagen an das aus Kohlenhydraten-bestehende O-Antigen des Lipopolysaccharids von Gram-negativen Bakterien. Das tailspike protein spaltet das O-Antigen um den Bakteriophage an die Oberfl{\"a}che des Bakteriums zu f{\"u}hren, damit eine Infektion stattfinden kann. Die Affinit{\"a}t des tailspike proteins zum O-Antigen ist dabei sehr niedrig, um nach Spaltung des O-Antigens das Spaltungsprodukt zu l{\"o}sen und wiederum neues Substrat zu binden. In dieser Arbeit wurde ein tailspike protein des Bakteriophagen Sf6 verwendet (Sf6 TSP), das spezifisch an das O-Antigen von Shigella flexneri Y bindet. Eine inaktive Variante des Sf6 TSP wurde verwendet um einen hoch-affin bindenden Sensor f{\"u}r pathogene Shigella zu entwickeln. Der Shigella-Sensor wurde durch Kopplung von unterschiedlichen Proteinmutanten mit einem fluoreszierendem Molek{\"u}l erhalten. Dabei zeigte eine dieser Mutanten bei Bindung von Shigella O-Antigen ein Fluoreszenz-Signal im Bereich des sichtbaren Lichts. Molekulardynamische Simulationen wurde anhand der erzeugten Proteinmutanten als Methode zum rationalen Design von hoch-affin Kohlenhydrat-bindenden Proteinen getestet und die resultierenden Affinit{\"a}tsvorhersagen wurden {\"u}ber Oberfl{\"a}chenplasmonresonanz-Experimente {\"u}berpr{\"u}ft. Aus weiteren experimentellen und simulierten Daten konnten schließlich Schlussfolgerungen {\"u}ber die Urspr{\"u}nge von Kohlenhydrat-Protein Interaktionen gezogen werden, die eine Einsicht {\"u}ber den Einfluss von Wasser in diesem Bindungsprozess lieferten.},
  language  = {en}
}
@misc{KuehnelKupfer2017,
  author    = {Kuehnel, Susanne and Kupfer, Alexander},
  title     = {Sperm storage in caecilian amphibians},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-400987},
  pages     = {5},
  year      = {2017},
  abstract  = {Background: Female sperm storage has evolved independently multiple times among vertebrates to control reproduction in response to the environment. In internally fertilising amphibians, female salamanders store sperm in cloacal spermathecae, whereas among anurans sperm storage in oviducts is known only in tailed frogs. Facilitated through extensive field sampling following historical observations we tested for sperm storing structures in the female urogenital tract of fossorial, tropical caecilian amphibians. Findings: In the oviparous Ichthyophis cf. kohtaoensis, aggregated sperm were present in a distinct region of the posterior oviduct but not in the cloaca in six out of seven vitellogenic females prior to oviposition. Spermatozoa were found most abundantly between the mucosal folds. In relation to the reproductive status decreased amounts of sperm were present in gravid females compared to pre-ovulatory females. Sperm were absent in females past oviposition. Conclusions: Our findings indicate short-term oviductal sperm storage in the oviparous Ichthyophis cf. kohtaoensis. We assume that in female caecilians exhibiting high levels of parental investment sperm storage has evolved in order to optimally coordinate reproductive events and to increase fitness.},
  language  = {en}
}
@article{KuecuekgoezeTeraoGarattinietal.2017,
  author    = {Kuecuekgoeze, Goekhan and Terao, Mineko and Garattini, Enrico and Leimk{\"u}hler, Silke},
  title     = {Direct Comparison of the Enzymatic Characteristics and Superoxide Production of the Four Aldehyde Oxidase Enzymes Present in Mouse},
  series = {Drug metabolism and disposition : the biological fate of chemicals},
  volume    = {45},
  journal   = {Drug metabolism and disposition : the biological fate of chemicals},
  publisher = {American Society for Pharmacology and Experimental Therapeutics},
  address   = {Bethesda},
  issn      = {0090-9556},
  doi       = {10.1124/dmd.117.075937},
  pages     = {947 -- 955},
  year      = {2017},
  abstract  = {Aldehyde oxidases (AOXs) are molybdoflavoenzymes with an important role in the metabolism and detoxification of heterocyclic compounds and aliphatic as well as aromatic aldehydes. The enzymes use oxygen as the terminal electron acceptor and produce reduced oxygen species during turnover. Four different enzymes, mAOX1, mAOX3, mAOX4, and mAOX2, which are the products of distinct genes, are present in the mouse. A direct and simultaneous comparison of the enzymatic properties and characteristics of the four enzymes has never been performed. In this report, the four catalytically active mAOX enzymes were purified after heterologous expression in Escherichia coli. The kinetic parameters of the four mouse AOX enzymes were determined and compared with the use of six predicted substrates of physiologic and toxicological interest, i.e., retinaldehyde, N1-methylnicotinamide, pyridoxal, vanillin, 4-(dimethylamino) cinnamaldehyde (p-DMAC), and salicylaldehyde. While retinaldehyde, vanillin, p-DMAC, and salycilaldehyde are efficient substrates for the four mouse AOX enzymes, N1-methylnicotinamide is not a substrate of mAOX1 or mAOX4, and pyridoxal is notmetabolized by any of the purified enzymes. Overall, mAOX1, mAOX2, mAOX3, and mAOX4 are characterized by significantly different KM and kcat values for the active substrates. The four mouse AOXs are also characterized by quantitative differences in their ability to produce superoxide radicals. With respect to this last point, mAOX2 is the enzyme generating the largest rate of superoxide radicals of around 40\% in relation to moles of substrate converted, and mAOX1, the homolog to the human enzyme, produces a rate of approximately 30\% of superoxide radicals with the same substrate.},
  language  = {en}
}
@phdthesis{Kubsch2017,
  author    = {Kubsch, Bastian},
  title     = {Phase-specific fusion between biomembranes using SNARE mimetics},
  school      = {Universit{\"a}t Potsdam},
  pages     = {95},
  year      = {2017},
  language  = {en}
}
@phdthesis{Kruse2017,
  author    = {Kruse, Stefan},
  title     = {Larix treeline dynamics in northern Siberia inferred from population genetics and individual-based modelling},
  school      = {Universit{\"a}t Potsdam},
  pages     = {181},
  year      = {2017},
  language  = {en}
}
@article{KrauzeKaempfHornetal.2017,
  author    = {Krauze, Patryk and K{\"a}mpf, Horst and Horn, Fabian and Liu, Qi and Voropaev, Andrey and Wagner, Dirk and Alawi, Mashal},
  title     = {Microbiological and Geochemical Survey of CO2-Dominated Mofette and Mineral Waters of the Cheb Basin, Czech Republic},
  series = {Frontiers in microbiology},
  volume    = {8},
  journal   = {Frontiers in microbiology},
  publisher = {Frontiers Research Foundation},
  address   = {Lausanne},
  issn      = {1664-302X},
  doi       = {10.3389/fmicb.2017.02446},
  pages     = {16},
  year      = {2017},
  abstract  = {The Cheb Basin (NW Bohemia, Czech Republic) is a shallow, neogene intracontinental basin. It is a non-volcanic region which features frequent earthquake swarms and large-scale diffuse degassing of mantle-derived CO2 at the surface that occurs in the form of CO2-rich mineral springs and wet and dry mofettes. So far, the influence of CO2 degassing onto the microbial communities has been studied for soil environments, but not for aquatic systems. We hypothesized, that deep-trenching CO2 conduits interconnect the subsurface with the surface. This admixture of deep thermal fluids should be reflected in geochemical parameters and in the microbial community compositions. In the present study four mineral water springs and two wet mofettes were investigated through an interdisciplinary survey. The waters were acidic and differed in terms of organic carbon and anion/cation concentrations. Element geochemical and isotope analyses of fluid components were used to verify the origin of the fluids. Prokaryotic communities were characterized through quantitative PCR and Illumina 16S rRNA gene sequencing. Putative chemolithotrophic, anaerobic and microaerophilic organisms connected to sulfur (e.g., Sulfuricurvum, Sulfurimonas) and iron (e.g., Gallionella, Sideroxydans) cycling shaped the core community. Additionally, CO2-influenced waters form an ecosystem containing many taxa that are usually found in marine or terrestrial subsurface ecosystems. Multivariate statistics highlighted the influence of environmental parameters such as pH, Fe2+ concentration and conductivity on species distribution. The hydrochemical and microbiological survey introduces a new perspective on mofettes. Our results support that mofettes are either analogs or rather windows into the deep biosphere and furthermore enable access to deeply buried paleo-sediments.},
  language  = {en}
}
@misc{KramerLenhard2017,
  author    = {Kramer, Elena M. and Lenhard, Michael},
  title     = {Shape and form in plant development},
  series = {Seminars in cell \& developmental biology},
  volume    = {79},
  journal   = {Seminars in cell \& developmental biology},
  publisher = {Elsevier},
  address   = {London},
  issn      = {1084-9521},
  doi       = {10.1016/j.semcdb.2017.11.004},
  pages     = {1 -- 2},
  year      = {2017},
  language  = {en}
}
@misc{KozielHermanussenGomulaetal.2017,
  author    = {Koziel, Slawomir and Hermanussen, Michael and Gomula, Alexandra and Swanson, James and Kaczmarek, Maria and El-Shabrawi, Mortada and Elhusseini, Mona and Satake, Takashi and Martinovic Klaric, Irena and Scheffler, Christiane and Morkuniene, Ruta and Godina, Elena and Sasa, Missoni and Tutkuviene, Janina and Siniarska, Anna and Nieczuja-Dwojacka, Joanna and Nunez, Javier and Groth, Detlef and Barbieri, Davide},
  title     = {Adolescence - a Transition to Adulthood Proceedings of the 24th Aschauer Soiree, held at Jurata, Poland, November 5th 2016},
  series = {Pediatric Endocrinology Reviews},
  volume    = {14},
  journal   = {Pediatric Endocrinology Reviews},
  number    = {3},
  publisher = {Medical Media},
  address   = {Netanya},
  issn      = {1565-4753},
  pages     = {326 -- 334},
  year      = {2017},
  abstract  = {Eighteen scientists met at Jurata, Poland, to discuss various aspects of the transition from adolescence to adulthood. This transition is a delicate period facing complex interactions between the adolescents and the social group they belong to. Social identity, group identification and identity signalling, but also stress affecting basal salivary cortisol rhythms, hypertension, inappropriate nutrition causing latent and manifest obesity, moreover, in developing and under-developed countries, parasitosis causing anaemia thereby impairing growth and development, are issues to be dealt with during this period of the human development. In addition, some new aspects of the association between weight, height and head circumference in the newborns were discussed, as well as intrauterine head growth and head circumference as health risk indicators.},
  language  = {en}
}
@misc{KoussoroplisSchwarzenbergerWacker2017,
  author    = {Koussoroplis, Apostolos-Manuel and Schwarzenberger, Anke and Wacker, Alexander},
  title     = {Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-395661},
  pages     = {7},
  year      = {2017},
  abstract  = {We studied the short- (12 h) and long-term (144 h) response of Daphnia pulex lipases to quality shifts in diets consisting of different mixtures of the green alga Scenedesmus with the cyanobacterium Synechococcus, two species with contrasting lipid compositions. The lipase/esterase activity in both the gut and the body tissues had fast responses to the diet shift and increased with higher dietary contributions of Synechococcus. When screening the Daphnia genome for TAG lipases, we discovered a large gene-family expansion of these enzymes. We used a subset of eight genes for mRNA expression analyses and distinguished between influences of time and diet on the observed gene expression patterns. We identified five diet-responsive lipases of which three showed a sophisticated short- and long-term pattern of expression in response to small changes in food-quality. Furthermore, the gene expression of one of the lipases was strongly correlated to lipase/esterase activity in the gut suggesting its potentially major role in digestion. These findings demonstrate that the lipid-related enzymatic machinery of D. pulex is finely tuned to diet and might constitute an important mechanism of physiological adaptation in nutritionally complex environments.},
  language  = {en}
}
@article{KoussoroplisSchwarzenbergerWacker2017,
  author    = {Koussoroplis, Apostolos-Manuel and Schwarzenberger, Anke and Wacker, Alexander},
  title     = {Diet quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex},
  series = {Biology open : BiO},
  volume    = {6},
  journal   = {Biology open : BiO},
  publisher = {The company of Biologists},
  address   = {Cambridge},
  doi       = {10.1242/bio.022046},
  pages     = {210 -- 216},
  year      = {2017},
  abstract  = {We studied the short- (12 h) and long-term (144 h) response of Daphnia pulex lipases to quality shifts in diets consisting of different mixtures of the green alga Scenedesmus with the cyanobacterium Synechococcus, two species with contrasting lipid compositions. The lipase/esterase activity in both the gut and the body tissues had fast responses to the diet shift and increased with higher dietary contributions of Synechococcus. When screening the Daphnia genome for TAG lipases, we discovered a large gene-family expansion of these enzymes. We used a subset of eight genes for mRNA expression analyses and distinguished between influences of time and diet on the observed gene expression patterns. We identified five diet-responsive lipases of which three showed a sophisticated short- and long-term pattern of expression in response to small changes in food-quality. Furthermore, the gene expression of one of the lipases was strongly correlated to lipase/esterase activity in the gut suggesting its potentially major role in digestion. These findings demonstrate that the lipid-related enzymatic machinery of D. pulex is finely tuned to diet and might constitute an important mechanism of physiological adaptation in nutritionally complex environments.},
  language  = {en}
}
@article{KoussoroplisPincebourdeWacker2017,
  author    = {Koussoroplis, Apostolos-Manuel and Pincebourde, Sylvain and Wacker, Alexander},
  title     = {Understanding and predicting physiological performance of organisms in fluctuating and multifactorial environments},
  series = {Ecological monographs : a publication of the Ecological Society of America.},
  volume    = {87},
  journal   = {Ecological monographs : a publication of the Ecological Society of America.},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {0012-9615},
  doi       = {10.1002/ecm.1247},
  pages     = {178 -- 197},
  year      = {2017},
  abstract  = {Understanding how variance in environmental factors affects physiological performance, population growth, and persistence is central in ecology. Despite recent interest in the effects of variance in single biological drivers, such as temperature, we have lacked a comprehensive framework for predicting how the variances and covariances between multiple environmental factors will affect physiological rates. Here, we integrate current theory on variance effects with co-limitation theory into a single unified conceptual framework that has general applicability. We show how the framework can be applied (1) to generate mathematically tractable predictions of the physiological effects of multiple fluctuating co-limiting factors, (2) to understand how each co-limiting factor contributes to these effects, and (3) to detect mechanisms such as acclimation or physiological stress when they are at play. We show that the statistical covariance of co-limiting factors, which has not been considered before, can be a strong driver of physiological performance in various ecological contexts. Our framework can provide powerful insights on how the global change-induced shifts in multiple environmental factors affect the physiological performance of organisms.},
  language  = {en}
}
@article{KolkNaafWulf2017,
  author    = {Kolk, Jens and Naaf, Tobias and Wulf, Monika},
  title     = {Paying the colonization credit},
  series = {Biodiversity and conservation},
  volume    = {26},
  journal   = {Biodiversity and conservation},
  publisher = {Springer},
  address   = {Dordrecht},
  issn      = {0960-3115},
  doi       = {10.1007/s10531-016-1271-y},
  pages     = {735 -- 755},
  year      = {2017},
  abstract  = {Massive historical land cover changes in the Central European lowlands have resulted in a forest distribution that now comprises small remnants of ancient forests and more recently established post-agricultural forests. Here, land-use history is considered a key driver of recent herb-layer community changes, where an extinction debt in ancient forest remnants and/or a colonization credit in post-agricultural forests are being paid over time. On a regional scale, these payments should in theory lead toward a convergence in species richness between ancient and post-agricultural forests over time. In this study, we tested this assumption with a resurvey of 117 semi-permanent plots in the well-studied deciduous forests of the Prignitz region (Brandenburg, NE Germany), where we knew that the plant communities of post-agricultural stands exhibit a colonization credit while the extinction debt in ancient stands has largely been paid. We compared changes in the species richness of all herb layer species, forest specialists and ancient forest indicator species between ancient and post-agricultural stands with linear mixed effect models and determined the influence of patch connectivity on the magnitude of species richness changes. Species richness increased overall, but the richness of forest specialists increased significantly more in post-agricultural stands and was positively influenced by higher patch connectivity, indicating a convergence in species richness between the ancient and postagricultural stands. Furthermore, the richness of ancient forest indicator species only increased significantly in post-agricultural stands. For the first time, we were able to verify a gradual payment of the colonization credit in post-agricultural forest stands using a comparison of actual changes in temporal species richness.},
  language  = {en}
}
@misc{KocyanWilandSzymanska2017,
  author    = {Kocyan, Alexander and Wiland-Szymanska, Justyna},
  title     = {A new name and a new combination for Friedmannia nom. illeg. (Hypoxidaceae)},
  series = {Phytotaxa : a rapid international journal for accelerating the publication of botanical taxonomy},
  volume    = {291},
  journal   = {Phytotaxa : a rapid international journal for accelerating the publication of botanical taxonomy},
  number    = {3},
  publisher = {Magnolia Press},
  address   = {Auckland},
  issn      = {1179-3155},
  doi       = {10.11646/phytotaxa.291.3.10},
  pages     = {239 -- 239},
  year      = {2017},
  abstract  = {Recently, Kocyan \& Wiland-Szymańska (2016) have published a thorough research article on one of the outstanding members of the family Hypoxidaceae on the Seychelles, which resulted in the raise of a new genus (Friedmannia Kocyan \& Wiland-Szymańska 2016: 60) to accommodate the former Curculigo seychellensis Bojer ex Baker (1877: 368). However, it has turned out that the name Friedmannia Chantanachat \& Bold (1962: 45) already exists in literature for a green alga, which renders the new hypoxid genus illegitimate (Melbourne Code; McNeill et al. 2012). Therefore, we assign a new generic epithet to Curculigo seychellensis.},
  language  = {en}
}
@article{KocJanuchtaHoefflerThomaetal.2017,
  author    = {Koc-Januchta, Marta and H{\"o}ffler, Tim and Thoma, Gun-Brit and Prechtl, Helmut and Leutner, Detlev},
  title     = {Visualizers versus verbalizers},
  series = {Computers in human behavior},
  volume    = {68},
  journal   = {Computers in human behavior},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {0747-5632},
  doi       = {10.1016/j.chb.2016.11.028},
  pages     = {170 -- 179},
  year      = {2017},
  abstract  = {This study was conducted in order to examine the differences between visualizers and verbalizers in the way they gaze at pictures and texts while learning. Using a collection of questionnaires, college students were classified according to their visual or verbal cognitive style and were asked to learn about two different, in terms of subject and type of knowledge, topics by means of text-picture combinations. Eye-tracking was used to investigate their gaze behavior. The results show that visualizers spent significantly more time inspecting pictures than verbalizers, while verbalizers spent more time inspecting texts. Results also suggest that both visualizers' and verbalizers' way of learning is active but mostly within areas providing the source of information in line with their cognitive style (pictures or text). Verbalizers tended to enter non-informative, irrelevant areas of pictures sooner than visualizers. The comparison of learning outcomes showed that the group of visualizers achieved better results than the group of verbalizers on a comprehension test.},
  language  = {en}
}
@misc{KniggeWengerBieretal.2017,
  author    = {Knigge, Xenia and Wenger, C. and Bier, Frank Fabian and H{\"o}lzel, Ralph},
  title     = {AC electrokinetic immobilisation of nanoobjects as individual singles in regular arrays},
  series = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  volume    = {46},
  journal   = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  publisher = {Springer},
  address   = {New York},
  issn      = {0175-7571},
  pages     = {S187 -- S187},
  year      = {2017},
  language  = {en}
}
@phdthesis{Knecht2017,
  author    = {Knecht, Volker},
  title     = {Modeling Biomolecular Association},
  school      = {Universit{\"a}t Potsdam},
  pages     = {297},
  year      = {2017},
  language  = {en}
}
@article{KloseRolkeBaumann2017,
  author    = {Klose, Sascha Peter and Rolke, Daniel and Baumann, Otto},
  title     = {Morphogenesis of honeybee hypopharyngeal gland during pupal development},
  series = {Frontiers in zoology},
  volume    = {14},
  journal   = {Frontiers in zoology},
  publisher = {BioMed Central},
  address   = {London},
  issn      = {1742-9994},
  doi       = {10.1186/s12983-017-0207-z},
  year      = {2017},
  abstract  = {Background The hypopharyngeal gland of worker bees contributes to the production of the royal jelly fed to queens and larvae. The gland consists of thousands of two-cell units that are composed of a secretory cell and a duct cell and that are arranged in sets of about 12 around a long collecting duct. Results By fluorescent staining, we have examined the morphogenesis of the hypopharyngeal gland during pupal life, from a saccule lined by a pseudostratified epithelium to the elaborate organ of adult worker bees. The hypopharyngeal gland develops as follows. (1) Cell proliferation occurs during the first day of pupal life in the hypopharyngeal gland primordium. (2) Subsequently, the epithelium becomes organized into rosette-like units of three cells. Two of these will become the secretory cell and the duct cell of the adult secretory units; the third cell contributes only temporarily to the development of the secretory units and is eliminated by apoptosis in the second half of pupal life. (3) The three-cell units of flask-shaped cells undergo complex changes in cell morphology. Thus, by mid-pupal stage, the gland is structurally similar to the adult hypopharyngeal gland. (4) Concomitantly, the prospective secretory cell attains its characteristic subcellular organization by the invagination of a small patch of apical membrane domain, its extension to a tube of about 100 μm in length (termed a canaliculus), and the expansion of the tube to a diameter of about 3 μm. (6) Finally, the canaliculus-associated F-actin system becomes reorganized into rings of bundled actin filaments that are positioned at regular distances along the membrane tube. Conclusions The morphogenesis of the secretory units in the hypopharyngeal gland of the worker bee seems to be based on a developmental program that is conserved, with slight modification, among insects for the production of dermal glands. Elaboration of the secretory cell as a unicellular seamless epithelial tube occurs by invagination of the apical membrane, its extension likely by targeted exocytosis and its expansion, and finally the reorganisation of the membrane-associated F-actin system. Our work is fundamental for future studies of environmental effects on hypopharyngeal gland morphology and development.},
  language  = {en}
}
@misc{KloseRolkeBaumann2017,
  author    = {Klose, Sascha Peter and Rolke, Daniel and Baumann, Otto},
  title     = {Morphogenesis of honeybee hypopharyngeal gland during pupal development},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-395712},
  pages     = {14},
  year      = {2017},
  abstract  = {Background The hypopharyngeal gland of worker bees contributes to the production of the royal jelly fed to queens and larvae. The gland consists of thousands of two-cell units that are composed of a secretory cell and a duct cell and that are arranged in sets of about 12 around a long collecting duct. Results By fluorescent staining, we have examined the morphogenesis of the hypopharyngeal gland during pupal life, from a saccule lined by a pseudostratified epithelium to the elaborate organ of adult worker bees. The hypopharyngeal gland develops as follows. (1) Cell proliferation occurs during the first day of pupal life in the hypopharyngeal gland primordium. (2) Subsequently, the epithelium becomes organized into rosette-like units of three cells. Two of these will become the secretory cell and the duct cell of the adult secretory units; the third cell contributes only temporarily to the development of the secretory units and is eliminated by apoptosis in the second half of pupal life. (3) The three-cell units of flask-shaped cells undergo complex changes in cell morphology. Thus, by mid-pupal stage, the gland is structurally similar to the adult hypopharyngeal gland. (4) Concomitantly, the prospective secretory cell attains its characteristic subcellular organization by the invagination of a small patch of apical membrane domain, its extension to a tube of about 100 μm in length (termed a canaliculus), and the expansion of the tube to a diameter of about 3 μm. (6) Finally, the canaliculus-associated F-actin system becomes reorganized into rings of bundled actin filaments that are positioned at regular distances along the membrane tube. Conclusions The morphogenesis of the secretory units in the hypopharyngeal gland of the worker bee seems to be based on a developmental program that is conserved, with slight modification, among insects for the production of dermal glands. Elaboration of the secretory cell as a unicellular seamless epithelial tube occurs by invagination of the apical membrane, its extension likely by targeted exocytosis and its expansion, and finally the reorganisation of the membrane-associated F-actin system. Our work is fundamental for future studies of environmental effects on hypopharyngeal gland morphology and development.},
  language  = {en}
}
@article{KloseRolkeBaumann2017,
  author    = {Klose, Sascha Peter and Rolke, Daniel and Baumann, Otto},
  title     = {Morphogenesis of honeybee hypopharyngeal gland during pupal development},
  series = {Frontiers in zoology},
  volume    = {14},
  journal   = {Frontiers in zoology},
  publisher = {BioMed Central},
  address   = {London},
  issn      = {1742-9994},
  doi       = {10.1186/s12983-017-0207-z},
  pages     = {2866 -- 2875},
  year      = {2017},
  abstract  = {Background: The hypopharyngeal gland of worker bees contributes to the production of the royal jelly fed to queens and larvae. The gland consists of thousands of two-cell units that are composed of a secretory cell and a duct cell and that are arranged in sets of about 12 around a long collecting duct. Results: By fluorescent staining, we have examined the morphogenesis of the hypopharyngeal gland during pupal life, from a saccule lined by a pseudostratified epithelium to the elaborate organ of adult worker bees. The hypopharyngeal gland develops as follows. (1) Cell proliferation occurs during the first day of pupal life in the hypopharyngeal gland primordium. (2) Subsequently, the epithelium becomes organized into rosette-like units of three cells. Two of these will become the secretory cell and the duct cell of the adult secretory units; the third cell contributes only temporarily to the development of the secretory units and is eliminated by apoptosis in the second half of pupal life. (3) The three-cell units of flask-shaped cells undergo complex changes in cell morphology. Thus, by mid-pupal stage, the gland is structurally similar to the adult hypopharyngeal gland. (4) Concomitantly, the prospective secretory cell attains its characteristic subcellular organization by the invagination of a small patch of apical membrane domain, its extension to a tube of about 100 mu m in length (termed a canaliculus), and the expansion of the tube to a diameter of about 3 mu m. (6) Finally, the canaliculus-associated F-actin system becomes reorganized into rings of bundled actin filaments that are positioned at regular distances along the membrane tube. Conclusions: The morphogenesis of the secretory units in the hypopharyngeal gland of the worker bee seems to be based on a developmental program that is conserved, with slight modification, among insects for the production of dermal glands. Elaboration of the secretory cell as a unicellular seamless epithelial tube occurs by invagination of the apical membrane, its extension likely by targeted exocytosis and its expansion, and finally the reorganisation of the membrane-associated F-actin system. Our work is fundamental for future studies of environmental effects on hypopharyngeal gland morphology and development.},
  language  = {en}
}