@article{GhylinGarciaMoyaetal.2014, author = {Ghylin, Trevor W. and Garcia, Sarahi L. and Moya, Francisco and Oyserman, Ben O. and Schwientek, Patrick and Forest, Katrina T. and Mutschler, James and Dwulit-Smith, Jeffrey and Chan, Leong-Keat and Martinez-Garcia, Manuel and Sczyrba, Alexander and Stepanauskas, Ramunas and Grossart, Hans-Peter and Woyke, Tanja and Warnecke, Falk and Malmstrom, Rex and Bertilsson, Stefan and McMahon, Katherine D.}, title = {Comparative single-cell genomics reveals potential ecological niches for the freshwater acl Actinobacteria lineage}, series = {The ISME journal : multidisciplinary journal of microbial ecology}, volume = {8}, journal = {The ISME journal : multidisciplinary journal of microbial ecology}, number = {12}, publisher = {Nature Publ. Group}, address = {London}, issn = {1751-7362}, doi = {10.1038/ismej.2014.135}, pages = {2503 -- 2516}, year = {2014}, abstract = {Members of the acI lineage of Actinobacteria are the most abundant microorganisms in most freshwater lakes; however, our understanding of the keys to their success and their role in carbon and nutrient cycling in freshwater systems has been hampered by the lack of pure cultures and genomes. We obtained draft genome assemblies from 11 single cells representing three acI tribes (acI-A1, acI-A7, acI-B1) from four temperate lakes in the United States and Europe. Comparative analysis of acI SAGs and other available freshwater bacterial genomes showed that acI has more gene content directed toward carbohydrate acquisition as compared to Polynucleobacter and LD12 Alphaproteobacteria, which seem to specialize more on carboxylic acids. The acI genomes contain actinorhodopsin as well as some genes involved in anaplerotic carbon fixation indicating the capacity to supplement their known heterotrophic lifestyle. Genome-level differences between the acI-A and acI-B clades suggest specialization at the clade level for carbon substrate acquisition. Overall, the acI genomes appear to be highly streamlined versions of Actinobacteria that include some genes allowing it to take advantage of sunlight and N-rich organic compounds such as polyamines, di-and oligopeptides, branched-chain amino acids and cyanophycin. This work significantly expands the known metabolic potential of the cosmopolitan freshwater acI lineage and its ecological and genetic traits.}, language = {en} } @article{LeunertEckertPauletal.2014, author = {Leunert, Franziska and Eckert, Werner and Paul, Andrea and Gerhardt, Volkmar and Grossart, Hans-Peter}, title = {Phytoplankton response to UV-generated hydrogen peroxide from natural organic matter}, series = {Journal of plankton research}, volume = {36}, journal = {Journal of plankton research}, number = {1}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {0142-7873}, doi = {10.1093/plankt/fbt096}, pages = {185 -- 197}, year = {2014}, abstract = {In aquatic systems, natural organic matter (NOM) and in particular humic substances effectively absorb the ultraviolet (UV)/visible light spectrum of solar radiation and act as a photoprotective filter for organisms. Simultaneously, UV contributes to the generation of potentially harmful reactive oxygen species (ROS). Dose-response experiments were conducted on cyanobacteria and green algae with hydrogen peroxide (H2O2) as a long-lived representative of ROS. Delayed fluorescence (DF) decay kinetics was used as a non-invasive tool to follow changes of phytoplankton activity in real time. In order to investigate phototoxicity and photoprotection by NOM on phytoplankton, we exposed algae to UV-pre-irradiated NOM and direct UV excitation. Cyanobacteria responded to H2O2 concentrations as low as 10(-7) M, while green algae were 2 orders of magnitude less sensitive. UV irradiation of medium with NOM generated H2O2 concentrations of 1.5 x 10(-7) to 3.6 x 10(-7) M. When exposed to these concentrations, only the DF of cyanobacteria led to a measurable effect while that of green algae did not change. The addition of NOM protected all phytoplankton from direct UV irradiation, but cyanobacteria benefitted less. From this we conclude that UV-irradiated water enriched with NOM can adversely affect the physiology of cyanobacteria, but not of green algae, which might control phytoplankton composition and species-specific activities.}, language = {en} } @article{TadaGrossart2014, author = {Tada, Yuya and Grossart, Hans-Peter}, title = {Community shifts of actively growing lake bacteria after N-acetyl-glucosamine addition: improving the BrdU-FACS method}, series = {The ISME journal : multidisciplinary journal of microbial ecology}, volume = {8}, journal = {The ISME journal : multidisciplinary journal of microbial ecology}, number = {2}, publisher = {Nature Publ. Group}, address = {London}, issn = {1751-7362}, doi = {10.1038/ismej.2013.148}, pages = {441 -- 454}, year = {2014}, abstract = {In aquatic environments, community dynamics of bacteria, especially actively growing bacteria (AGB), are tightly linked with dissolved organic matter (DOM) quantity and quality. We analyzed the community dynamics of DNA-synthesizing and accordingly AGB by linking an improved bromodeoxyuridine immunocytochemistry approach with fluorescence-activated cell sorting (BrdU-FACS). FACS-sorted cells of even oligotrophic ecosystems in winter were characterized by 16S rRNA gene analysis. In incubation experiments, we examined community shifts of AGB in response to the addition of N-acetyl-glucosamine (NAG), one of the most abundant aminosugars in aquatic systems. Our improved BrdU-FACS analysis revealed that AGB winter communities of oligotrophic Lake Stechlin (northeastern Germany) substantially differ from those of total bacteria and consist of Alpha-, Beta-, Gamma-, Deltaproteobacteria, Actinobacteria, Candidatus OP10 and Chloroflexi. AGB populations with different BrdU-fluorescence intensities and cell sizes represented different phylotypes suggesting that single-cell growth potential varies at the taxon level. NAG incubation experiments demonstrated that a variety of widespread taxa related to Alpha-, Beta-, Gammaproteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, Planctomycetes, Spirochaetes, Verrucomicrobia and Chloroflexi actively grow in the presence of NAG. The BrdU-FACS approach enables detailed phylogenetic studies of AGB and, thus, to identify those phylotypes which are potential key players in aquatic DOM cycling.}, language = {en} } @article{TangMcGinnisFrindteetal.2014, author = {Tang, Kam W. and McGinnis, Daniel F. and Frindte, Katharina and Bruchert, Volker and Grossart, Hans-Peter}, title = {Paradox reconsidered: Methane oversaturation in well-oxygenated lake waters}, series = {Limnology and oceanography}, volume = {59}, journal = {Limnology and oceanography}, number = {1}, publisher = {Wiley}, address = {Waco}, issn = {0024-3590}, doi = {10.4319/lo.2014.59.1.0275}, pages = {275 -- 284}, year = {2014}, abstract = {The widely reported paradox of methane oversaturation in oxygenated water challenges the prevailing paradigm that microbial methanogenesis only occurs under anoxic conditions. Using a combination of field sampling, incubation experiments, and modeling, we show that the recurring mid-water methane peak in Lake Stechlin, northeast Germany, was not dependent on methane input from the littoral zone or bottom sediment or on the presence of known micro-anoxic zones. The methane peak repeatedly overlapped with oxygen oversaturation in the seasonal thermocline. Incubation experiments and isotope analysis indicated active methane production, which was likely linked to photosynthesis and/or nitrogen fixation within the oxygenated water, whereas lessening of methane oxidation by light allowed accumulation of methane in the oxygen-rich upper layer. Estimated methane efflux from the surface water was up to 5 mmol m(-2) d(-1). Mid-water methane oversaturation was also observed in nine other lakes that collectively showed a strongly negative gradient of methane concentration within 0-20\% dissolved oxygen (DO) in the bottom water, and a positive gradient within >= 20\% DO in the upper water column. Further investigation into the responsible organisms and biochemical pathways will help improve our understanding of the global methane cycle.}, language = {en} }