@article{DunsingLucknerZuehlkeetal.2018,
  author    = {Dunsing, Valentin and Luckner, Madlen and Zuehlke, Boris and Petazzi, Roberto Arturo and Herrmann, Andreas and Chiantia, Salvatore},
  title     = {Optimal fluorescent protein tags for quantifying protein oligomerization in living cells},
  series = {Scientific reports},
  volume    = {8},
  journal   = {Scientific reports},
  publisher = {Nature Publ. Group},
  address   = {London},
  issn      = {2045-2322},
  doi       = {10.1038/s41598-018-28858-0},
  pages     = {12},
  year      = {2018},
  abstract  = {Fluorescence fluctuation spectroscopy has become a popular toolbox for non-disruptive analysis of molecular interactions in living cells. The quantification of protein oligomerization in the native cellular environment is highly relevant for a detailed understanding of complex biological processes. An important parameter in this context is the molecular brightness, which serves as a direct measure of oligomerization and can be easily extracted from temporal or spatial fluorescence fluctuations. However, fluorescent proteins (FPs) typically used in such studies suffer from complex photophysical transitions and limited maturation, inducing non-fluorescent states. Here, we show how these processes strongly affect molecular brightness measurements. We perform a systematic characterization of non-fluorescent states for commonly used FPs and provide a simple guideline for accurate, unbiased oligomerization measurements in living cells. Further, we focus on novel red FPs and demonstrate that mCherry2, an mCherry variant, possesses superior properties with regards to precise quantification of oligomerization.},
  language  = {en}
}
@misc{LucknerDunsingChiantiaetal.2018,
  author    = {Luckner, Madlen and Dunsing, Valentin and Chiantia, Salvatore and Hermann, Andreas},
  title     = {Oligomerization and nuclear shuttling dynamics of viral proteins studied by quantitative molecular brightness analysis using fluorescence correlation spectroscopy},
  series = {Biophysical journal},
  volume    = {114},
  journal   = {Biophysical journal},
  number    = {3},
  publisher = {Cell Press},
  address   = {Cambridge},
  issn      = {0006-3495},
  doi       = {10.1016/j.bpj.2017.11.1951},
  pages     = {350A -- 350A},
  year      = {2018},
  language  = {en}
}
@misc{LucknerDunsingChiantiaetal.2017,
  author    = {Luckner, Madlen and Dunsing, Valentin and Chiantia, Salvatore and Herrmann, Andreas},
  title     = {Influenza virus vRNPs: quantitative investigations via fluorescence cross-correlation spectroscopy},
  series = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  volume    = {46},
  journal   = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  publisher = {Springer},
  address   = {New York},
  issn      = {0175-7571},
  pages     = {S368 -- S368},
  year      = {2017},
  language  = {en}
}
@misc{LucknerDunsingDruekeetal.2019,
  author    = {Luckner, Madlen and Dunsing, Valentin and Dr{\"u}ke, Markus and Zuehlke, B. and Petazzi, Roberto Arturo and Chiantia, Salvatore and Herrmann, A.},
  title     = {Quantifying protein oligomerization directly in living cells},
  series = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  volume    = {48},
  journal   = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  publisher = {Springer},
  address   = {New York},
  issn      = {0175-7571},
  pages     = {S183 -- S183},
  year      = {2019},
  language  = {en}
}