@misc{Kehr2005,
  author    = {Kehr, Julia},
  title     = {Untersuchungen der Proteine in den Langstreckentransportsystemen h{\"o}herer Pflanzen},
  address   = {Potsdam},
  pages     = {45 Bl. ; graph. Darst.},
  year      = {2005},
  language  = {de}
}
@article{GroblerHartlGrobleretal.2005,
  author    = {Grobler, J. P. and Hartl, G. B. and Grobler, N. and Kotze, A. and Botha, K. and Tiedemann, Ralph},
  title     = {The genetic status of an isolated black wildebeest (Connochaetes gnou) population from the Abe Bailey Nature Reserve, South Africa : Microsatellite data on a putative past hybridization with blue wildebeest (C-taurinus)},
  issn      = {1616-5047},
  year      = {2005},
  abstract  = {The present study aimed at assessing genetic purity of black wildebeest (Connochoetes gnou) at Abe Bailey Nature Reserve, Gauteng Province, South Africa, using a multitocus microsatellite approach. Five loci were studied in black and blue (C. taurinus) wildebeest, the latter being a closely related species and known to produce hybrids with the morphologically very similar black wildebeest. In fact, the entire national black wildebeest population of South Africa potentially contains a significant proportion of introgressed blue wildebeest genes. In our case, eight out of 39 alleles were unique to black and 22 to blue wildebeest, with nine alleles shared between pure populations of the two species in Line with their taxonomic proximity. A possible Limited past introgression of blue wildebeest genes into the Abe Bailey population, corresponding to documents on population history, was only supported by the presence of a single allele otherwise exclusively found in samples of four pure blue but not in samples of two pure black wildebeest control populations. However, an assignment test and coefficients of population divergence did not support an extended introgression of C. taurinus alleles into the C. gnou population under study. Average heterozygosity at Abe Bailey proved to be intermediate between black and blue wildebeest, the tatter species generally harbouring more genetic variation than the former owing to larger population sizes and the absence of population bottlenecks in historical times. The implications of our data are discussed with reference to the persistence of introgressed genes and the conservation of pure black wildebeest gene pools},
  language  = {en}
}
@article{VasseurGaedkeMcCann2005,
  author    = {Vasseur, David and Gaedke, Ursula and McCann, Kevin S.},
  title     = {A seasonal alternation of coherent and compensatory dynamics occurs in phytoplankton},
  year      = {2005},
  abstract  = {Functional groups with diverse responses to environmental factors sum to produce communities with less temporal variability in their biomass than those lacking this diversity. The detection of these compensatory dynamics can be complicated by a spatio-temporal alternation in the environmental factors limiting growth (both abiotic and biotic), which restricts the occurrence of compensatory dynamics to certain periods or locations. Hence, resolving the spatio- temporal scale may uncover important spatial and/or temporal components in community variability. Using long-term data from Lake Constance (Bodensee), we find that a reduction in grazing pressure and relaxed competition for nutrients during winter and spring generates coherent dynamics among edible and less edible phytoplankton. During summer and fall, when both grazing pressure and nutrient limitation are present, edible and less edible phytoplankton exhibit compensatory dynamics. This study supports recent work suggesting that both abiotic and biotic interactions promote compensatory dynamics and to our knowledge, this is the first example of a system where compensatory and coherent dynamics seasonally alternate.},
  language  = {en}
}
@article{HeilmannGrothBehrsingetal.2005,
  author    = {Heilmann, Katja and Groth, Thomas and Behrsing, Olaf and Wagner, Albrecht and Schossig-Tiedemann, Michael and Lendlein, Andreas and Micheel, Burkhard},
  title     = {The influence of the chemical composition of cell culture material on the growth and antibody production of hybridoma cells},
  year      = {2005},
  abstract  = {The multiplication and antibody production of murine hybridoma cells cultured on five different polymer membranes were tested and compared with conventional tissue culture polystyrene (TCPS). Membranes were prepared from polyacrylonitrile (PAN) and acrylonitrile copolymerized with N-vinylpyrrolidone (NVP20, NVP30), Na-methallylsulfonate (NaMAS) and N-(3-amino-propyl-methacrylamide-hydrochloride) (APMA). Cell number and antibody concentration were quantified as criteria for viability and productivity. Adhesion of hybridoma cells was characterized by vital and scanning electron microscopy. The results suggest that a strong adhesion of cells, observed on APMA and TCPS, increased cell growth but reduced monoclonal antibody production. In contrast membranes with lowered adhesivity such as NVP20 provided favourable conditions for monoclonal antibody production. In addition it was shown that this membrane also possessed a minor fouling as indicated by the low decrease of water flux across the membrane after protein adsorption. It was concluded that NVP20 could be a suitable material for the development of hollow fibre membranes for bioreactors.},
  language  = {en}
}
@article{SchmollDietrichWinkeletal.2005,
  author    = {Schmoll, Tim and Dietrich, Volkmar and Winkel, Wolfgang and Epplen, J. T. and Schurr, Frank Martin and Lubjuhn, Thomas},
  title     = {Paternal genetic effects on offspring fitness are context dependent within the extrapair mating system of a socially monogamous passerine},
  year      = {2005},
  abstract  = {Avian extrapair mating systems provide an interesting model to assess the role of genetic benefits in the evolution of female multiple mating behavior, as potentially confounding nongenetic benefits of extrapair mate choice are seen to be of minor importance. Genetic benefit models of extrapair mating behavior predict that females engage in extrapair copulations with males of higher genetic quality compared to their social mates, thereby improving offspring reproductive value. The most straightforward test of such good genes models of extrapair mating implies pail-wise comparisons of maternal half-siblings raised in the same environment, which permits direct assessment of Paternal genetic effects oil offspring traits. But genetic benefits of mate choice may be difficult to detect. Furthermore, the extent of genetic benefits (in terms of increased offspring viability or fecundity) may depend oil the environmental context Such that the proposed differences between extrapair offspring (EPO) and within-pair offspring (WPO) only appear under comparatively poor environmental conditions. We tested the hypothesis that genetic benefits of female extrapair mate choice are context dependent by analyzing offspring fitness-related traits in the coal tit (Parus ater) in relation to seasonal variation in environmental conditions. Paternal genetic effects on offspring fitness were context dependent. as shown by a significant interaction effect of differential paternal genetic contribution and offspring hatching date. EPO showed a higher local recruitment probability than their maternal half-siblings if born comparatively late in the season (i.e.. when overall performance had significantly declined), while WPO performed better early in the season. The same general pattern of context dependence was evident when using the number of grandchildren born to a cuckolding female via her female WPO or EPO progeny as the respective fitness measure. However, we were unable to demonstrate that cuckolding females obtained a general genetic fitness benefit from extrapair fertilizations in terms of offspring viability or fecundity. Thus, another type of benefit Could be responsible for maintaining female extrapair mating preferences in the study population. Our results suggest that more than a single selective pressure may have shaped the evolution of female extrapair mating behavior in socially monogamous passerines.},
  language  = {en}
}
@article{Kummer2005,
  author    = {Kummer, Volker},
  title     = {Beitr{\"a}ge zur Pilzflora der Luckauer Umgebung : die Ergebnisse der Pilzexkursion am 10.10.2004 im Bereich des Drehnaer Weinberges},
  year      = {2005},
  language  = {de}
}
@article{WillivanBuskirkFischer2005,
  author    = {Willi, Yvonne and van Buskirk, J. and Fischer, Markus},
  title     = {A threefold genetic allee effect : Population size affects cross-compatibility, inbreeding depression and drift load in the self-incompatible Ranunculus reptans},
  issn      = {0016-6731},
  year      = {2005},
  language  = {en}
}
@phdthesis{Bistolas2005,
  author    = {Bistolas, Nikitas},
  title     = {Investigation of the direct heterogenous electron transfer of hemoglobin and cytochrome P450 enzymes and bioanalytical application},
  address   = {Potsdam},
  pages     = {IX, 120 S. : graph. Darst.},
  year      = {2005},
  language  = {en}
}
@phdthesis{Bari2005,
  author    = {Bari, Rajendra P.},
  title     = {Molecular characterization of phosphate signaling in arabidopsis thaliana},
  address   = {Potsdam},
  pages     = {X, 141 S. : graph. Darst.},
  year      = {2005},
  language  = {en}
}
@article{FussmannBlasius2005,
  author    = {Fussmann, Gregor F. and Blasius, Bernd},
  title     = {Community response to enrichment is highly sensitive to model structure},
  year      = {2005},
  abstract  = {Biologists use mathematical functions to model, understand, and predict nature. For most biological processes, however, the exact analytical form is not known. This is also true for one of the most basic life processes, the uptake of food or resources. We show that the use of a number of nearly indistinguishable functions, which can serve as phenomenological descriptors of resource uptake, may lead to alarmingly different dynamical behaviour in a simple community model. More specifically, we demonstrate that the degree of resource enrichment needed to destabilize the community dynamics depends critically on the mathematical nature of the uptake function.},
  language  = {en}
}
@phdthesis{Arrivault2005,
  author    = {Arrivault, St{\´e}phanie},
  title     = {Functional characterization of Arabidopsis thaliana MTP3, a putative metal transport protein of the cation diffusion facilitator (CDF) family},
  pages     = {VII, 121 Bl. : Ill., graph. Darst.},
  year      = {2005},
  language  = {en}
}
@phdthesis{Wilczek2005,
  author    = {Wilczek, Sabine},
  title     = {Spatial and seasonal distribution of extracellular enzyme activities in the River Elbe and their regulation by envirommental variables},
  pages     = {114 S. : Ill., graph. Darst.},
  year      = {2005},
  language  = {en}
}
@phdthesis{Gaude2005,
  author    = {Gaude, Nicole},
  title     = {Die Regulation der Galaktolipidbiosynthese unter verschiedenen Umweltbedingungen in Arabidopsis thaliana, Glycine max und Lotus japonicus},
  pages     = {106 Bl. : Ill., graph. Darst.},
  year      = {2005},
  language  = {de}
}
@phdthesis{Schad2005,
  author    = {Schad, Martina},
  title     = {Systemische Auswirkungen der kompatiblen Interaktion zwischen Golovinomyces orontii und Arabidopsis thaliana auf die molekulare Zusammensetzung von Leitgeweben},
  address   = {Potsdam},
  pages     = {92 Bl. : graph. Darst.},
  year      = {2005},
  language  = {de}
}
@phdthesis{Lisso2005,
  author    = {Lisso, Janina},
  title     = {Molekulare Analyse der Brassionsteroid-Wirkung in Pflanzen},
  address   = {Potsdam},
  pages     = {100 Bl. : graph. Darst.},
  year      = {2005},
  language  = {de}
}
@phdthesis{Koetting2005,
  author    = {K{\"o}tting, Oliver},
  title     = {Ein neues st{\"a}rke-relevantes Enzym in Arabidopsis thaliana: die Phosphoglukan-Wasser-Dikinase},
  publisher = {Rhombos-Verl.},
  address   = {Berlin},
  isbn      = {3-937231-89-7},
  pages     = {177 S. : graph. Darst.},
  year      = {2005},
  language  = {de}
}
@phdthesis{Shirokova2005,
  author    = {Shirokova, Elena},
  title     = {Functional genomics of olfactory and pheromone receptors by reconstitution of their signal transduction in human HeLa cells},
  address   = {Potsdam},
  pages     = {83 S. : graph. Darst.},
  year      = {2005},
  language  = {en}
}
@phdthesis{Bruemmel2005,
  author    = {Br{\"u}mmel, Yvonne},
  title     = {Nanoskalige, kristalline Fluoresceindiacetat-Partikel als Verst{\"a}rkungssysteme f{\"u}r Fluoreszenz- Immunoassays : Einfluss von Gr{\"o}ße und Oberfl{\"a}chenfunktionalisierung},
  pages     = {X, 113 Bl.},
  year      = {2005},
  language  = {de}
}
@phdthesis{Moeller2005,
  author    = {M{\"o}ller, Jens},
  title     = {Besiedlung naturnaher W{\"a}lder unterschiedlicher Isolation durch Tiergruppen verschiedener Mobilit{\"a}t},
  address   = {Potsdam},
  pages     = {129 S. : graph. Darst., Kt.},
  year      = {2005},
  language  = {de}
}
@article{Kummer2005,
  author    = {Kummer, Volker},
  title     = {Bemerkenswerte Pilzfunde auf der 35. Brandenburgischen Botanikertagung in Storkow},
  year      = {2005},
  language  = {de}
}
@phdthesis{Schulze2005,
  author    = {Schulze, Torsten},
  title     = {Changes in structure and function of the fish community in a mesotrophic lake ecosystem after stocking a non- native top predator},
  pages     = {108 S. : graph. Darst.},
  year      = {2005},
  language  = {en}
}
@phdthesis{Zippel2005,
  author    = {Zippel, Barbara},
  title     = {Einfluss von Intraguild Predation auf die Dynamik der Planktonsukzession in einem sauren Bergbausee},
  address   = {Potsdam},
  pages     = {82 S. : graph. Darst.},
  year      = {2005},
  language  = {de}
}
@phdthesis{Wollenberger2005,
  author    = {Wollenberger, Ursula},
  title     = {Kopplung von Biomolek{\"u}len mit Elektroden : von Bioelektrochemie zur Biosensorik},
  address   = {Potsdam},
  pages     = {Getr. Z{\"a}hlung : graph. Darst.},
  year      = {2005},
  language  = {de}
}
@phdthesis{Puetz2005,
  author    = {P{\"u}tz, Sandro},
  title     = {Long-Term Impact of grazing on vegetation under stochastic climate : a cross-scale simulation study},
  pages     = {VI, 162, XXXIII Bl. : Ill., graph. Darst.},
  year      = {2005},
  language  = {en}
}
@phdthesis{Boehme2005,
  author    = {B{\"o}hme, Carsten},
  title     = {Validierung der Messung der Oberfl{\"a}chenkontur der Lendenwirbels{\"a}ule},
  pages     = {Vii, 57 Bl.},
  year      = {2005},
  language  = {de}
}
@article{BlenauBaumann2005,
  author    = {Blenau, Wolfgang and Baumann, Arnd},
  title     = {Molecular characterization of the ebony gene from the American cockroach, Periplaneta americana},
  year      = {2005},
  abstract  = {Biogenic amines are an important class of primary messengers in the central (CNS) and peripheral nervous systems and in peripheral organs. These substances regulate and modulate many physiological and behavioral processes. Various inactivation mechanisms for these substances exist to terminate biogenic amine-mediated signal transduction. In vertebrates, the enzymes monoamine oxidase and/or catechol-O-methyl-transferase are involved in these processes. In insects, however, in which both enzymes are low in abundance or absent, biogenic amines are inactivated mainly by N- acetylation or O-sulphation. In Droso-philo, beta-alanyl conjugation mediated by the Ebony protein has recently been shown to be a novel and alternative pathway for biogenic amine inactivation. Here, we report the cloning of ebony cDNA (Peaebony) from a brain-specific cDNA library of the cockroach Periplaneta americana. The open reading frame encodes a protein of 860 amino acid residues (PeaEbony). The PeaEbony polypeptide shares homology to Ebony sequences from Anopheles gambiae, Apis mellifera, and Drosophila melonogaster. In addition, PeaEbony exhibits sequence similarity to a family of microbial non-ribosomal peptide synthetases. The mRNA encoding PeaEbony is highly expressed in the cockroach brain and to a lesser extent in the salivary glands. PeaEbony is, therefore, probably involved in the inactivation of various biogenic amines through beta-alanyl conjugation in the cockroach CNS. Since the salivary glands in Periplaneta are innervated by dopaminergic and serotonergic neurons, PeaEbony probably also biochemically modifies dopamine and serotonin in these acinar glands. Arch. Insect Biochem. (c) 2005 Wiley-Liss, Inc},
  language  = {en}
}
@article{BogenBenderSchlenstedtetal.2005,
  author    = {Bogen, Oliver and Bender, Olaf and Schlenstedt, Jana and Goswami, C. and Schroder, W. and Blenau, Wolfgang and Gillen, C. and Dreger, M. and Hucho, F.},
  title     = {Neuronal expression of the IB4-binding versican},
  issn      = {0022-3042},
  year      = {2005},
  language  = {en}
}
@article{BarbierOesterheltLarsonetal.2005,
  author    = {Barbier, Guillaume and Oesterhelt, Christine and Larson, Matthew D. and Halgren, Robert G. and Wilkerson, Curtis and Garavito, Michael R. and Benning, Christoph and Weber, Andreas P. M.},
  title     = {Comparative genomics of two closely related unicellular thermo-acidophilic red algae, Galdieria sulphuraria and Cyanidioschyzon merolae, reveals the molecular basis of the metabolic flexibility of Galdieria sulphuraria and sigant differences in carbohydrate metabolism of both algae},
  year      = {2005},
  abstract  = {Unicellular algae serve as models for the study and discovery of metabolic pathways, for the functional dissection of cell biological processes such as organellar division and cell motility, and for the identification of novel genes and gene functions. The recent completion of several algal genome sequences and expressed sequence tag collections and the establishment of nuclear and organellar transformation methods has opened the way for functional genomics approaches using algal model systems. The thermo-acidophilic unicellular red alga Galdieria sulphuraria represents a particularly interesting species for a genomics approach owing to its extraordinary metabolic versatility such as heterotrophic and mixotrophic growth on more than 50 different carbon sources and its adaptation to hot acidic environments. However, the ab initio prediction of genes required for unknown metabolic pathways from genome sequences is not trivial. A compelling strategy for gene identification is the comparison of similarly sized genomes of related organisms with different physiologies. Using this approach, candidate genes were identified that are critical to the metabolic versatility of Galdieria. Expressed sequence tags and high-throughput genomic sequence reads covering >70\% of the G. sulphuraria genome were compared to the genome of the unicellular, obligate photoautotrophic red alga Cyanidioschyzon merolae. More than 30\% of the Galdieria sequences did not relate to any of the Cyandioschyzon genes. A closer inspection of these sequences revealed a large number of membrane transporters and enzymes of carbohydrate metabolism that are unique to Galdieria. Based on these data, it is proposed that genes involved in the uptake of reduced carbon compounds and enzymes involved in their metabolism are crucial to the metabolic flexibility of G. sulphuraria},
  language  = {en}
}
@article{BinzenhoeferSchroderStraussetal.2005,
  author    = {Binzenh{\"o}fer, Birgit and Schroder, B. and Strauss, B. and Biedermann, Robert and Settele, Josef},
  title     = {Habitat models and habitat connectivity analysis for butterflies and burnet moths : the example of Zygaena carniolica and Coenonympha arcania},
  year      = {2005},
  abstract  = {In this paper, habitat models were used to predict potential habitat for endangered species, which is an important question in landscape and conservation planning. Based on logistic regression, we developed habitat distribution models for the burnet moth Zygaena carniolica and the nymphalid butterfly Coenonympha arcania in Northern Bavaria, Germany. The relation between adult occurrence and habitat parameters, including the influence of landscape context, was analyzed on, 118 sites. Habitat connectivity analyses were carried out on the basis of (1) habitat suitability maps generated from these models and (2) dispersal data from mark recapture studies. Our results showed that (1) the presence of the burnet depended mainly on the presence of nectar plants and of nutrient-poor dry grasslands in direct vicinity, that of the nymphalid on larger areas of extensively used dry grasslands within 100 m vicinity in combination with small patches of higher shrubs and bushes. (2) Internal as well as external validation indicated the robustness and general applicability of the models. Transferability in time and space indicated their high potential relevance for applications in nature conservation, such as predicting possible effects of land use changes. (3) Habitat connectivity analyses revealed a high degree of habitat connectivity within the study area. Thus, we could show no effects of isolation or habitat size for both species. (c) 2005 Elsevier Ltd. All rights reserved},
  language  = {en}
}
@article{BoelJurrmannBrigeliusFlohe2005,
  author    = {B{\"o}l, Gaby Fleur and Jurrmann, Nadine and Brigelius-Floh{\´e}, Regina},
  title     = {Cellular trafficking of the IL-1RI-associated kinase-1 requires intact kinase activity},
  issn      = {0006-291X},
  year      = {2005},
  abstract  = {Upon stimulation of cells with interleukin-1 (IL-1) the IL-1 receptor type 1 (IL-1RI) associated kinase-1 (IRAK- 1) transiently associates to and dissociates front the IL-IRI and thereafter translocates into the nucleus. Here we show that nuclear translocation of IRAK-I depends on its kinase activity since translocation was not observed in EL-4 cells overexpressing a kinase negative IRAK-1 mutant (EL-4(IRAK-1-K239S)). IRAK-1 itself, an endogenous substrate with an apparent molecular weight of 24 kDa (p24). and exogenous substrates like histone and myelin basic protein are phosphorylated by nuclear located IRAK-1. Phosphorylation of p24 cannot be detected in EL-4(IRAK-1-K239S) cells. IL-1- dependent recruitment of IRAK-1 to the IL-1RI and subsequent phosphorylation of IRAK-l is a prerequisite for nuclear translocation of IRAK-1. It is therefore concluded that intracellular localization of IRAK-1 depends on its kinase activity and that IRAK-1 may also function as a kinase in the nucleus as shown by a new putative endogenous substrate. (c) 2005 Elsevier Inc. All rights reserved},
  language  = {en}
}
@article{LangeHimmelAuerbachetal.2005,
  author    = {Lange, Stephan and Himmel, Mirko and Auerbach, Daniel and Agarkova, Irina and Hayess, Katrin and F{\"u}rst, Dieter Oswald and Perriard, Jean-Claude and Ehler, Elisabeth},
  title     = {Dimerisation of myomesin : implications for the structure of the sarcomeric M-band},
  issn      = {0022-2836},
  year      = {2005},
  abstract  = {The sarcomeric M-band is thought to provide a link between the thick and the elastic, filament systems. So far, relatively little is known about its structural components and their three-dimensional organisation. Myomesin seems to be an essential component of the M-band, since it is expressed in all types of vertebrate striated muscle fibres investigated and can be found in its mature localisation pattern as soon as the first myofibrils are assembled. Previous work has shown that the N-terminal and central part of myomesin harbour binding sites for myosin, titin and muscle creatine kinase. Intrigued by the highly conserved domain layout of the C-terminal half, we screened for new interaction partners by yeast two-hybrid analysis. This revealed a strong interaction of myomesin with itself. This finding was confirmed by several biochemical assays. Our data suggest that myomesin can form antiparallel dimers via a binding site residing in its C-terminal domain 13. We suggest that, similar to alpha-actinin in the Z-disc, the myomesin dimers cross- link the contractile filaments in the M-band. The new and the already previously identified myomesin interaction sites are integrated into the first three-dimensional model of the sarcomeric M-band on a molecular basis. (C) 2004 Elsevier Ltd. All rights reserved},
  language  = {en}
}
@article{GoetzSuopankiSchuleretal.2005,
  author    = {Goetz, C. and Suopanki, J. and Schuler, Benjamin and Wanker, E. and Herrmann, Andreas},
  title     = {Perturbation of brain lipid membrane by soluble Huntingtin depends on its polyproline tract},
  issn      = {0006-3495},
  year      = {2005},
  language  = {en}
}
@article{BistolasWollenbergerJungetal.2005,
  author    = {Bistolas, Nikitas and Wollenberger, Ursula and Jung, Christiane and Scheller, Frieder W.},
  title     = {Cytochrome P450 biosensors : a review},
  year      = {2005},
  abstract  = {Cytochrome P450 (CYP) is a large family of enzymes containing heme as the active site. Since their discovery and the elucidation of their structure, they have attracted the interest of scientist for many years, particularly due to their catalytic abilities. Since the late 1970s attempts have concentrated on the construction and development of electrochemical sensors. Although sensors based on mediated electron transfer have also been constructed, the direct electron transfer approach has attracted most of the interest. This has enabled the investigation of the electrochemical properties of the various isoforms of CYP. Furthermore, CYP utilized to construct biosensors for the determination of substrates important in environmental monitoring, pharmaceutical industry and clinical practice. (c) 2004 Elsevier B. V. All rights reserved},
  language  = {en}
}
@article{HauswaldtGlenn2005,
  author    = {Hauswaldt, J. S. and Glenn, T. C.},
  title     = {Population genetics of the diamondback terrapin (Malaclemys terrapin)},
  issn      = {0962-1083},
  year      = {2005},
  abstract  = {We examined the population genetic structure of the diamondback terrapins (Malaclemys terrapin), within and among estuaries. Based on mark-recapture studies, these estuarine turtles have high site fidelity that is likely to make them vulnerable to local extinctions. We tested if observed site fidelity of adults would be reflected in intraestuarine population genetic structure of six highly polymorphic microsatellite loci (five tetranucleotide and one dinucleotide). No evidence was found for population structuring within the Charleston estuary nor among three different estuaries in South Carolina. We then examined four other terrapin populations from North Carolina to New York, as well as from the Florida Keys and from Texas. With increasing geographical distance, genetic differentiation increased from South Carolina through New York, but overall values were low. The dinucleotide locus contributed significantly more to the genetic differentiation of some population comparisons than any of the other loci. Interestingly, terrapins from South Carolina to New York were much more genetically similar to those from Texas (rho = 0.154) than to those from Florida (rho = 0.357). We attribute this pattern to extensive translocations of terrapins during the early 20th century to replenish diminished populations and to provide turtle farms with stocks. Terrapins collected in Texas were especially sought for shipment to the northeastern US because of their larger size. Our study indicates no population structure within or among adjacent estuaries. Thus, the mark-recapture information from adult and subadult feeding locations is a poor predictor of population genetic structure. Additionally, it appears that past human activities may have drastically altered the genetics of current populations. Finally, our data suggest that translocation of eggs or head starting of terrapins within estuaries or among adjacent estuaries is acceptable from a genetic standpoint},
  language  = {en}
}
@article{KetmaierMandatoriDeMatthaeisetal.2005,
  author    = {Ketmaier, Valerio and Mandatori, R. and De Matthaeis, E. and Mura, G.},
  title     = {Molecular systematics and phylogeography in the fairy shrimp Tanymastix stagnalis based on mitochondrial DNA},
  issn      = {0952-8369},
  year      = {2005},
  abstract  = {Patterns of sequence divergence in about 1 kb of mitochondrial DNA coding for two genes (16s rRNA and cytochrome oxidase I, COI) were analysed in 13 populations of the fairy shrimp Tanymastix stagnalis from Norway, Germany, France, Italy (northern and central Italy plus insular populations from Sardinia and the Tuscan Archipelago) and Spain, and in one presumed population of Tanymastix stellae from Corsica. The latter species was originally known only from a single locality in Sardinia, which has been destroyed by urbanization; the Corsican population was referred to T stellae by some French authors on the basis of the collection of several cysts from mud. mtDNA data revealed a very low level of genetic divergence between the presumed population of T stellae and the other T stagnalis populations included in the study. Our genetic findings do not support the presence of T stellae in Corsica and are in line with previous SEM studies revealing that all species belonging to the genus Tanymastix produce cysts with identical morphology. The results indicate complex phylogeographic relationships and pronounced genetic differentiation among T stagnalis populations. The islands of Corsica and Sardinia on the one hand and the island of Capraia (Tuscan Archipelago) on the other were probably colonized independently at different times. Genetic relationships among continental populations do not follow a clear geographical trend, indicating that geographical distance is not the main force shaping the pattern of genetic structuring of the species. Stochastic factors such as multiple and independent founder events probably contributed to the striking pattern of genetic differentiation along with subsequent local adaptation. These results agree with previously published molecular work on several groups of aquatic organisms and further support the high potential for dispersal-low gene flow paradox shown by a large array of animals living in lentic habitats},
  language  = {en}
}
@article{HummelKeshvariWeckwerthetal.2005,
  author    = {Hummel, Jan and Keshvari, N. and Weckwerth, Wolfram and Selbig, Joachim},
  title     = {Species-specific analysis of protein sequence motifs using mutual information},
  issn      = {1471-2105},
  year      = {2005},
  abstract  = {Background: Protein sequence motifs are by definition short fragments of conserved amino acids, often associated with a specific function. Accordingly protein sequence profiles derived from multiple sequence alignments provide an alternative description of functional motifs characterizing families of related sequences. Such profiles conveniently reflect functional necessities by pointing out proximity at conserved sequence positions as well as depicting distances at variable positions. Discovering significant conservation characteristics within the variable positions of profiles mirrors group-specific and, in particular, evolutionary features of the underlying sequences. Results: We describe the tool PROfile analysis based on Mutual Information (PROMI) that enables comparative analysis of user-classified protein sequences. PROMI is implemented as a web service using Perl and R as well as other publicly available packages and tools on the server-side. On the client-side platform-independence is achieved by generally applied internet delivery standards. As one possible application analysis of the zinc finger C2H2-type protein domain is introduced to illustrate the functionality of the tool. Conclusion: The web service PROMI should assist researchers to detect evolutionary correlations in protein profiles of defined biological sequences. It is available at http:// promi.mpimpgolm. mpg.de where additional documentation can be found},
  language  = {en}
}
@article{HalamekMakowerKnoescheetal.2005,
  author    = {Halamek, Jan and Makower, Alexander and Kn{\"o}sche, Kristina and Skladal, Petr and Scheller, Frieder W.},
  title     = {Piezoelectric affinity sensors for cocaine and cholinesterase inhibitors},
  year      = {2005},
  abstract  = {We report here the development of piezoelectric affinity sensors for cocaine and cholinesterase inhibitors based on the formation of affinity complexes between an immobilized cocaine derivative and an anti-cocaine antibody or cholinesterase. For both binding reactions benzoylecgonine-1,8-diamino-3,4-dioxaoctane (BZE-DADOO) was immobilized on the surface of the sensor. For immobilization. pre-conjugated BZE-DADOO with 11-mercaptomonoundecanoic acid (MUA) via 2- (5-norbornen-2,3-dicarboximide)-1,1,3,3-tetramethyluronium-tetrafluoro borate (TNTU) allowed the formation of a chemisorbed monolayer on the piezosensor surface. The detection of cocaine was based oil a competitive assay. The change of frequency measured after 300 s of the binding reaction was used as the signal. The maximum binding of the antibody resulted in a frequency decrease of 35 Hz (with an imprecision 3\%, n = 3) while the presence of 100 pmol I-1 cocaine decreased the binding by 11\%. The limit of detection was consequently below 100 pmol I-1 for cocaine. The total time of one analysis was 15 min. This BZE-DADOO-modified sensor was adapted for the detection of organophosphates. BZE-DADOO - a competitive inhibitor - served as binding element for cholinesterase in a competitive assay. (C) 2004 Elsevier B.V. All rights reserved},
  language  = {en}
}
@article{Halamek2005,
  author    = {Halamek, Jan},
  title     = {Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor},
  year      = {2005},
  abstract  = {A highly sensitive piezoelectric biosensor has been developed for detection of cholinesterase inhibitors. The inhibitor benzoylecgonine-1,8-diamino-3,4-dioxaoctane (BZE-DADOO) was immobilized on a monolayer of 11- mercaptomonoundecanoic acid (MUA) self-assembled on the gold surface of the sensor. The binding of high-molecular-weight cholinesterase to the immobilized cocaine derivative was monitored with a mass sensitive piezoelectric quartz crystal (quartz crystal nanobalance; QCN). In the presence of an inhibiting substance in the sample, the binding of cholinesterase to the immobilized inhibitor was reduced. The decrease of the rate of mass change was proportional to the concentration of free inhibitor in the sample. This way the affinity sensor followed anti-cholinesterase toxicity and the enzyme activity of ChE was not addressed. A assay for detection of organophosphates (OP) was optimized. Regeneration of the sensor surface was achieved with 1 mol L-1 formic acid, which enabled 40 measurements with one sensor. All assays were carried out in a flow-through arrangement. The total measurement time (binding + regeneration) was 25 min and the detection limit for different OP (paraoxon, diisopropylfluorophosphate, chlorpyriphos, and chlorfenvinphos) was down to 10(-10) mol L-1 (0.02 mu g L-1). This sensor was used for determination of organophosphate (diisopropylfluorophosphate) levels in river water samples},
  language  = {en}
}
@article{KamjunkeTittelKrumbecketal.2005,
  author    = {Kamjunke, Norbert and Tittel, J{\"o}rg and Krumbeck, H. and Beulker, Camilla and Poerschmann, J.},
  title     = {High heterotrophic bacterial production in acidic, iron-rich mining lakes},
  issn      = {0095-3628},
  year      = {2005},
  abstract  = {The acidic mining lakes of Eastern Germany are characterized by their extremely low pH and high iron concentrations. Low concentrations of CO2 in the epilimnion due to the low pH and reduced light transmission due to dissolved ferric iron potentially limit phytoplankton primary production (PP), whereas dissolved organic carbon (DOC) may promote heterotrophic production of bacteria (HP). We, therefore, tested whether HP exceeds PP in three lakes differing in pH and iron concentration (mean pH 2.3-3.0, 23-500 mg Fe L-1). Bacterial biomass and HP achieved highest values in the most acidic, most iron-rich lake, whereas PP was highest in the least acidic lake. HP was often higher than PP (ratio HP/PP up to 11), indicating that planktonic PP was not the main carbon source for the bacteria. HP was not related to PP and DOC, but HP as well as bacterial biomass increased with decreasing pH. Light stimulated the formation of ferrous iron, changed the DOC composition, and increased the HP in laboratory experiments, suggesting that iron photoreduction caused DOC degradation. This may explain why we found the highest HP in the most acidic and most rich lake. Overall, the importance of bacteria in the cycling of matter and as a basis for the whole food web seemed to increase in more acidic lakes with higher iron concentrations},
  language  = {en}
}
@article{KetmaierBernardini2005,
  author    = {Ketmaier, Valerio and Bernardini, C.},
  title     = {Structure of the mitochondrial control region of the Eurasian otter (Lutra lutra ; Carnivora, Mustelidae): patterns of genetic heterogeneity and implications for conservation of the species in Italy},
  issn      = {0022-1503},
  year      = {2005},
  abstract  = {In this study we determined the complete sequence of the mitochondrial DNA (mtDNA) control region of the Eurasian otter (Lutra lutra). We then compared these new sequences with orthologues of nine carnivores belonging to six families (Mustelidae, Mephitidae, Canidae, Hyaenidae, Ursidae, and Felidae). The comparative analyses identified all the conserved regions previously found in mammals. The Eurasian otter and seven other species have a single location with tandem repeats in the right domain, while the spotted hyena (Hyaenidae) and the tiger (Felidae) have repeated sequences in both the right and left domains. To assess the degree of genetic heterogeneity of the Eurasian otter in Italy we sequenced two fragments of the gene and analyzed length polymorphisms of repeated sequences and heteroplasmy in 32 specimens. The study includes 23 museum specimens collected in northern, central, and southern Italy; most of these specimens are from extinct populations, while the southern Italian samples belong to the sole extant Italian population of the Eurasian otter. The study also includes all the captive-reared animals living in the colony "Centro Lontra, Caramanico Terme" (Pescara, central Italy). The colony is maintained for reintroduction of the species. We found a low level of genetic polymorphism; a single haplotype is dominant, but our data indicate the presence in central and southern Italy of two slightly divergent haplotypes. One haplotype belongs to an extinct population, the other is present in the single extant Italian population. Analyses of length polymorphisms and heteroplasmy indicate that the autochthonous Italian samples are characterized by a distinct array of repeated sequences from captive-reared animals},
  language  = {en}
}
@article{SchurrBondMidgleyetal.2005,
  author    = {Schurr, Frank Martin and Bond, William J. and Midgley, Guy F. and Higgins, Steven I.},
  title     = {A mechanistic model for secondary seed dispersal by wind and its experimental validation},
  issn      = {0022-0477},
  year      = {2005},
  abstract  = {1 Secondary seed dispersal by wind, the wind-driven movement of seeds along the ground surface, is an important dispersal mechanism for plant species in a range of environments. 2 We formulate a mechanistic model that describes how secondary dispersal by wind is affected by seed traits, wind conditions and obstacles to seed movement. The model simulates the movement paths of individual seeds and can be fully specified using independently measured parameters. 3 We develop an explicit version of the model that uses a spatially explicit representation of obstacle patterns, and also an aggregated version that uses probability distributions to model seed retention at obstacles and seed movement between obstacles. The aggregated version is computationally efficient and therefore suited to large-scale simulations. It provides a very good approximation of the explicit version (R-2 > 0.99) if initial seed positions vary randomly relative to the obstacle pattern. 4 To validate the model, we conducted a field experiment in which we released seeds of seven South African Proteaceae species that differ in seed size and morphology into an arena in which we systematically varied obstacle patterns. When parameterized with maximum likelihood estimates obtained from independent measurements, the explicit model version explained 70-77\% of the observed variation in the proportion of seeds dispersed over 25 m and 67- 69\% of the observed variation in the direction of seed dispersal. 5 The model tended to underestimate dispersal rates, possibly due to the omission of turbulence from the model, although this could also be explained by imprecise estimation of one model parameter (the aerodynamic roughness length). 6 Our analysis of the aggregated model predicts a unimodal relationship between the distance of secondary dispersal by wind and seed size. The model can also be used to identify species with the potential for long-distance seed transport by secondary wind dispersal. 7 The validated model expands the domain of mechanistic dispersal models, contributes to a functional understanding of seed dispersal, and provides a tool for predicting the distances that seeds move},
  language  = {en}
}
@article{TirrellKatz2005,
  author    = {Tirrell, M. V. and Katz, A.},
  title     = {Self-assembly in materials synthesis},
  issn      = {0883-7694},
  year      = {2005},
  abstract  = {The synthesis of materials via self-assembly typically involves the spontaneous and reversible organization,of small building blocks for the purpose of creating conglomerate assembly structures over larger length scales. This introductory article, describes self processes on several length scales, from subnanometer, up to millimeter scales, and briefly summarizes some of the incredible diversity of materials that exhibit self-assembly. Articles in this issue cover self-assembly using zeolitic structures, organic molecular crystals; block copolymers, surfactants, mesoscale templates, and soluble. crystallization additives},
  language  = {en}
}
@article{TittelBissingerGaedkeetal.2005,
  author    = {Tittel, J{\"o}rg and Bissinger, Vera and Gaedke, Ursula and Kamjunke, Norbert},
  title     = {Inorganic carbon limitation and mixotrophic growth in Chlamydomonas from an acidic mining lake},
  issn      = {1434-4610},
  year      = {2005},
  abstract  = {Plankton communities in acidic mining lakes (pH 2.5-3.3) are species-poor because they face extreme environmental conditions, e.g. 150 mg l(-1) Fe2++Fe3+. We investigated the growth characteristics of the dominant pigmented species, the flagellate Chlamydomonas acidophila, in semi-continuous culture experiments under in situ conditions. The following hypotheses were tested: (1) Low inorganic carbon (IC) concentrations in the epilimnion (e.g. 0.3 mg l(-1)) arising from the low pH limit phototrophic growth (H-1); (2) the additional use of dissolved organic carbon (mixotrophy) leads to higher growth rates under IC-limitation (H-2), and (3) phagotrophy is not relevant (H-3). H- 1 was supported as the culture experiments, in situ PAR and IC concentrations indicated that IC potentially limited phototrophic growth in the mixed surface layers. H-2 was also supported: mixotrophic growth always exceeded pure phototrophic growth even when photosynthesis was saturated. Dark growth in filtered lake water illuminated prior to inoculation provided evidence that Chlamydomonas was able to use the natural DOC. The alga did not grow on bacteria, thus confirming H-3. Chlamydomonas exhibited a remarkable resistance to starvation in the dark. The compensation light intensity (ca. 20 mu mol photons m(-2) s(-1)) and the maximum phototrophic growth (1.50 d(-1)) fell within the range of algae from non-acidic waters. Overall, Chlamydomonas, a typical r-strategist in circum-neutral systems, showed characteristics of a K-strategist in the stable, acidic lake environment in achieving moderate growth rates and minimizing metabolic losses. (c) 2005 Elsevier GmbH. All rights reserved},
  language  = {en}
}
@article{RoggeItagakiFishkinetal.2005,
  author    = {Rogge, B. and Itagaki, Y. and Fishkin, N. and Levi, E. and Ruhl, R. and Yi, S. S. and Nakanishi, K. and Hammerling, U.},
  title     = {Retinoylserine and retinoylalanine, natural products of the moth Trichoplusia ni},
  issn      = {0163-3864},
  year      = {2005},
  abstract  = {Insect cells convert vitamin A into a number of retinoids that are evolutionarily conserved with those of mammalian cells. However, insect cells also produce additional natural retinoids. Namely, two retinoic acid peptides, N- trans-retinoylserine (1) and N-trans-retinoylalanine (2), have been isolated from a cell line of the common cabbage looper, Trichoplusia ni. These are the first examples of naturally occurring retinoic acid linked to amino acids through an amide bond; the amino acid moieties are depicted in the more common L-configuration, although the absolute configuration was not determined due to the minuscule sample amount},
  language  = {en}
}
@article{PieperFuerst2005,
  author    = {Pieper-F{\"u}rst, U.},
  title     = {Gold nanoparticle-enhanced surface plasmon resonance measurement with a highly sensitive quantification for human tissue inhibitor of metalloproteinases-2},
  year      = {2005},
  abstract  = {Two different methods for the quantification of human tissue inhibitor of metalloproteinases-2 (TIMP-2) were developed using surface plasmon resonance (SPR) and gold nanoparticles for signal enhancement. The first method, a competitive assay, used TIMP-2 immobilized to the sensor surface and the inactive form of matrix metalloproteinase-2 (proMMP-2) (EC 3.4.24.24) adsorbed to gold nanoparticles. The sensor signals resulting from the interaction of MMP-2- gold nanoparticles with immobilized TIMP-2 were inversely proportional to the amounts of TIMP-2 of the sample. The measuring range for TIMP-2 was about 15-180pM. The second method, a one-step sandwich assay, used proMMP-2 immobilized to the sensor surface and an anti-TIMP-2 monoclonal antibody coupled to gold nanoparticles. The lower detection limit of this assay format was 0.5 pM of TIMP-2. The binding signals were highly reproducible up to 100 pM of the inhibitor. The improvements obtained in TIMP-2 quantification over already existing tests could contribute to a better understanding and diagnosis of diseases like cancer. (c) 2005 Elsevier B.V. All rights reserved},
  language  = {en}
}
@article{PoreeWulfetangeNasoetal.2005,
  author    = {Poree, Fabien and Wulfetange, K. and Naso, A. and Carpaneto, Armando and Roller, A. and Natura, G. and Bertl, Adam and Sentenac, H. and Thibaud, Jean-Baptiste and Dreyer, Ingo},
  title     = {Plant K-in and K-out channels : Approaching the trait of opposite rectification by analyzing more than 250 KAT1- SKOR chimeras},
  issn      = {0006-291X},
  year      = {2005},
  abstract  = {Members of the Shaker-like plant K+ channel family share a common structure, but are highly diverse in their function: they behave as either hyperpolarization-activated inward-rectifying (K-in) channels, or leak-like (K-weak) channels, or depolarization-activated outward-rectifying (K-out) channels. Here we created 256 chimeras between the K-in channel KAT1 and the K-out channel SKOR. The chimeras were screened in a potassium-uptake deficient yeast strain to identify those, which mediate potassium inward currents, i.e., which are functionally equivalent to KAT1. This strategy allowed Lis to identify three chimeras which differ from KAT1 in three parts of the polypeptide: the cytosolic N- terminus, the cytosolic C-terminus, and the putative voltage-sensor S4. Additionally, mutations in the K-out Channel SKOR were generated in order to localize molecular entities underlying its depolarization activation. The triple mutant SKOR-D312N-M313L-1314G, carrying amino-acid changes in the S6 segment, was identified as a channel which did not display any rectification in the tested voltage-range. (C) 2005 Elsevier Inc. All rights reserved},
  language  = {en}
}
@article{RietdorfBlenauWalz2005,
  author    = {Rietdorf, Katja and Blenau, Wolfgang and Walz, Bernd},
  title     = {Protein secretion in cockroach salivary glands requires an increase in intracellular cAMP and Ca2+ concentrations},
  issn      = {0022-1910},
  year      = {2005},
  abstract  = {The salivary glands in the cockroach Periplaneta americana secrete protein-containing saliva when stimulated by serotonin (5-HT) and protein-free saliva upon dopamine stimulation. In order to obtain information concerning the signalling pathways involved in 5-HT-induced protein secretion, we have determined the protein content of saliva secreted after experimental manipulations that potentially elevate intracellular Ca2+ and cyclic nucleotide concentrations in isolated glands. We have found that 5-HT stimulates the rate of protein secretion in a dose-dependent manner (threshold: 3 x 10(-8) M; EC50 1.5 x 10(-6) M). The maximal rate of 5-HT-induced protein secretion was 2.2 +/- 0.2 mu g/min. Increasing intracellular Ca2+ or cAMP by bath application of ionomycin (5 mu M), db cAMP (10 mM), forskolin (100 mu M) or IBMX (100 mu M), respectively, stimulated protein secretion at significantly lower rates, whereas db cGMP (1 mM) did not activate protein secretion. The high rates and the kinetics of 5-HT-induced protein secretion could only be mimicked by either applying forskolin together with IBMX (with or without ionomycin) or by applying IBMX together with ionomycin. Our measurements suggest that 5-HT-induced protein secretion is mediated by an elevation of [cAMP](i) and that Ca2+ may function as a co-agonist and augment the rate of protein secretion. (c) 2005 Elsevier Ltd. All rights reserved},
  language  = {en}
}
@article{KellerSauerStraussetal.2005,
  author    = {Keller, S. and Sauer, I. and Strauss, H. and Gast, Klaus and Dathe, M. and Bienert, Michael C.},
  title     = {Membrane-mimetic nanocarriers formed by a dipalmitoylated cell-penetrating peptide},
  year      = {2005},
  language  = {en}
}
@article{SchellerLisdatWollenberger2005,
  author    = {Scheller, Frieder W. and Lisdat, Fred and Wollenberger, Ursula},
  title     = {Application of electrically contacted enzymes for biosensors},
  isbn      = {3-527- 30690-0},
  year      = {2005},
  language  = {en}
}
@article{ShpilyovZinchenkoShestakovetal.2005,
  author    = {Shpilyov, Alexey V. and Zinchenko, Vladislav V. and Shestakov, Sergey V. and Grimm, Bernhard and Lokstein, Heiko},
  title     = {Inactivation of the geranylgeranyl reductase (ChlP) gene in the cyanobacterium Synechocystis sp. PCC 6803},
  year      = {2005},
  language  = {en}
}