@article{WronskiWacherHammondetal.2010,
  author    = {Wronski, Torsten and Wacher, Timothy and Hammond, Robert L. and Winney, Bruce and Hundertmark, Kris J. and Blacket, Mark J. and Mohammed, Osama B. and Flores, Benito and Omer, Sawsan A. and Macasero, William and Plath, Martin and Tiedemann, Ralph and Bleidorn, Christoph},
  title     = {Two reciprocally monophyletic mtDNA lineages elucidate the taxonomic status of Mountain gazelles (Gazella gazella)},
  issn      = {1477-2000},
  doi       = {10.1080/14772001003613192},
  year      = {2010},
  abstract  = {Mountain gazelles (Gazella gazella) rank among the most critically endangered mammals on the Arabian Peninsula. Past conservation efforts have been plagued by confusion about the phylogenetic relationship among various 'phenotypically discernable' populations, and even the question of species boundaries was far from being certain. This lack of knowledge has had a direct impact on conservation measures, especially ex situ breeding programmes, hampering the assignment of captive stocks to potential conservation units. Here, we provide a phylogenetic framework, based on the analysis of mtDNA sequences (360 bp cytochrome b and 213 bp Control Region) of 126 individuals collected from the wild throughout the Arabian Peninsula and from captive stocks. Our analyses revealed two reciprocally monophyletic genetic lineages within the presumed species Gazella gazella: one 'northern clade' on the Golan Heights (Israel/Syrian border) and one genetically diverse larger clade from the rest of the Arabian Peninsula including the Arava Valley (Negev, Israel). Applying the Strict Phylogenetic Species Concept (sensu Mishler \& Theriot, 2000) allows assigning species status to these two major clades.},
  language  = {en}
}
@article{NeumannSchulteJuenemannetal.2006,
  author    = {Neumann, Meina and Schulte, Marc and J{\"u}nemann, Nora and St{\"o}cklein, Walter F. M. and Leimk{\"u}hler, Silke},
  title     = {Rhodobacter capsulatus XdhC is involved in molybdenum cofactor binding and insertion into xanthine dehydrogenase},
  doi       = {10.1074/jbc.M601617200},
  year      = {2006},
  abstract  = {Rhodobacter capsulatus xanthine dehydrogenase (XDH) is a cytoplasmic enzyme with an (alpha beta) 2 heterodimeric structure that is highly identical to homodimeric eukaryotic xanthine oxidoreductases. The crystal structure revealed that the molybdenum cofactor (Moco) is deeply buried within the protein. A protein involved in Moco insertion and XDH maturation has been identified, which was designated XdhC. XdhC was shown to be essential for the production of active XDH but is not a subunit of the purified enzyme. Here we describe the purification of XdhC and the detailed characterization of its role for XDH maturation. We could show that XdhC binds Moco in stoichiometric amounts, which subsequently can be inserted into Moco-free apo-XDH. A specific interaction between XdhC and XdhB was identified. We show that XdhC is required for the stabilization of the sulfurated form of Moco present in enzymes of the xanthine oxidase family. Our findings imply that enzyme-specific proteins exist for the biogenesis of molybdoenzymes, coordinating Moco binding and insertion into their respective target proteins. So far, the requirement of such proteins for molybdoenzyme maturation has been described only for prokaryotes},
  language  = {en}
}
@article{FengNiElgeetal.2006,
  author    = {Feng, Xiao-Li and Ni, Wei-Min and Elge, Stephan and M{\"u}ller-R{\"o}ber, Bernd and Xu, Zhi-Hong and Xue, Hong-Wei},
  title     = {Auxin flow in anther filaments is critical for pollen grain development through regulating pollen mitosis},
  issn      = {0167-4412},
  doi       = {10.1007/s11103-006-0005-z},
  year      = {2006},
  abstract  = {It was well known that auxin is critical for anther/pollen grain development, however, the clear distribution and detailed effects of auxin during floral development are still unclear. We have shown here that, through analyzing GUS activities of Arabidopsis lines harboring auxin response elements DR5-GUS, auxin was mainly accumulated in the anther during flower stages 10-12. Further studies employing the indoleacetic acid-lysine synthetase (iaaL) coding gene from Pseudomonas syringae subsp. savastanoi under control of the promoter region of Arabidopsis phosphatidylinositol monophosphate 5-kinase 1 gene, which conducts the anther filament-specific expression, showed that block of auxin flow of filaments resulted in shortened filaments and significantly defective pollen grains. Similar phenotype was observed in tobacco plants transformed with the same construct, confirming the effects of auxin flow in filaments on anther development. Detailed studies further revealed that the meiosis process of pollen grain was normal while the mitosis at later stage was significantly defected, indicating the effects of auxin flow in filaments on pollen grain mitosis process. Analysis employing [C-14]IAA, as well as the observation on the expression of AtPIN1, coding for auxin efflux carrier, demonstrated the presence of polar auxin transport in anther filaments and pollen grains},
  language  = {en}
}
@article{MikhailyukKnoxPaschenkoetal.2006,
  author    = {Mikhailyuk, Igor K. and Knox, Peter P. and Paschenko, Vladimir Z. and Razjivin, Andrej P. and Lokstein, Heiko},
  title     = {Analysis of absorption spectra of purple bacterial reaction centers in the near infrared region by higher order derivative spectroscopy},
  doi       = {10.1016/j.bpc.2006.02.002},
  year      = {2006},
  abstract  = {Reaction centers (RCs) of purple bacteria are uniquely suited objects to study the mechanisms of the photosynthetic conversion of light energy into chemical energy. A recently introduced method of higher order derivative spectroscopy [I.K. Mikhailyuk, H. Lokstein, A.P. Razjivin, A method of spectral subband decomposition by simultaneous fitting the initial spectrum and a set of its derivatives, J. Biochem. Biophys. Methods 63 (2005) 10-23] was used to analyze the NIR absorption spectra of RC preparations from Rhodobacter (R.) sphaeroides strain 2R and Blastochloris (B.) viridis strain KH, containing bacteriochlorophyll (BChl) a and b, respectively. Q(y) bands of individual RC porphyrin components (BChls and bacteriopheophytins, BPheo) were identified. The results indicate that the upper exciton level Py+ of the photo-active BChl dimer in RCs of R. sphaeroides has an absorption maximum of 810nm. The blue shift of a complex integral band at approximately 800nm upon oxidation of the RC is caused primarily by bleaching of Py+, rather than by an electrochromic shift of the absorption band(s) of the monomeric BChls. Likewise, the disappearance of a band peaking at 842 nm upon oxidation of RCs from B. viridis indicates that this band has to be assigned to Py+, A blue shift of an absorption band at approximately 830nm upon oxidation of RCs of B. viridis is also essentially caused by the disappearance of Py+, rather than by an electrochromic shift of the absorption bands of monomeric BChls. Absorption maxima of the monomeric BCHls, B-B and B-A are at 802 and 797nm, respectively, in RCs of R. sphaeroides at room temperature. BPheo co-factors H-B and HA peak at 748 and 758 nm, respectively, at room temperature. For B. viridis RCs the spectral positions of HB and HA were found to be 796 and 816nm, respectively, at room temperature.},
  language  = {en}
}
@article{WronskiTiedemannApioetal.2006,
  author    = {Wronski, Torsten and Tiedemann, Ralph and Apio, Ann and Plath, Martin},
  title     = {Cover, food, competitors and individual densities within bushbuck Tragelaphus scriptus female clan home ranges},
  year      = {2006},
  abstract  = {We examined factors predicting female densities within the common home ranges of related females ("clans") in bushbuck Tragelaphus scriptus Pallas, 1776. In this species, each female forms an individual home range, but the home ranges of matrilineal clan members strongly overlap. We found female densities to increase in areas with high canopy cover. Moreover, individual home range sizes tended to decrease with increasing cover. Food plant availability and intruder pressure by two heterospecific competitors did not significantly affect female densities. Apparently, canopy cover is the major limited resource in this species. A possible explanation is that both adult bushbuck and - even more markedly - fawns hide from predators in dense vegetation, in particular in thicket clumps and coalescences. The study shows an effect of habitat properties (eg sufficient canopy cover) on a within-population level in bushbuck, where female densities differ even among proximate clan areas},
  language  = {en}
}
@article{MorgenthalWeckwerthSteuer2006,
  author    = {Morgenthal, Katja and Weckwerth, Wolfram and Steuer, Ralf},
  title     = {Metabolomic networks in plants : transitions from pattern recognition to biological interpretation},
  doi       = {10.1016/j.biosystems.2005.05.017},
  year      = {2006},
  abstract  = {Nowadays techniques for non-targeted metabolite profiling allow for the generation of huge amounts of relevant data essential for the construction of dynamic metabolomic networks. Thus, metabolomics, besides transcriptomics or proteomics, provides a major tool for the characterization of postgenomic processes. In this work, we introduce comparative correlation analysis as a complementary approach to characterize the physiological states of various organs of diverse plant species with focus on specific participation of metabolites in different reaction networks. The correlations observed are induced by diminutive fluctuations in environmental conditions, which propagate through the system and induce specific patterns depending on the genomic background. In order to examine this hypothesis, numeric examples of such fluctuations are computed and compared with experimentally obtained metabolite data.},
  language  = {en}
}
@article{PlathSeggelBurmeisteretal.2006,
  author    = {Plath, Martin and Seggel, Uta and Burmeister, Heike and Heubel, Katja U. and Schlupp, Ingo},
  title     = {Choosy males from the underground : male mating preferences in surface- and cave-dwelling Atlantic mollies (Poecilia mexicana)},
  doi       = {10.1007/s00114-005-0072-z},
  year      = {2006},
  abstract  = {Atlantic mollies (Poecilia mexicana) inhabit a variety of surface habitats, but they also occur in a sulfur cave in southern Mexico. We examined male mate choice relative to female body size in the cave population and in the most closely related surface-dwelling population from a nearby river. Males from both populations were either light- or dark-reared and could choose between two differently sized females either on the basis of visual cues in light or on the basis of solely nonvisual cues in darkness. Sexual preferences were estimated from the degree of association. Cave molly males always showed a preference for the larger female, both in light and in darkness. Among the surface males, only light-reared males showed a preference in the visual cues test, but not in darkness. In a control experiment, we demonstrated that male association preferences directly translate into actual mating preferences. Apparently, using visual cues for mate choice is the ancestral state in this system, and using nonvisual cues has evolved as a novel trait in the cave population. We discuss the evolution of nonvisual male mate choice in the context of changed environmental conditions, namely the absence of light, hypoxia, and toxic hydrogen sulfide in the cave},
  language  = {en}
}
@article{StalzRothSchleuderetal.2006,
  author    = {Stalz, Holger and Roth, Udo and Schleuder, Detlev and Macht, Marcus and Haebel, Sophie and Strupat, Kerstin and Peter-Katalinic, Jasna and Hanisch, Franz-Georg},
  title     = {The Geodia cydonium galectin exhibits prototype and chimera-type characteristics and a unique sequence polymorphism within its carbohydrate recognition domain},
  doi       = {10.1093/glycob/cwj086},
  year      = {2006},
  abstract  = {The ancestral galectin from the sponge Geodia cydonium (GCG) is classified on a structural basis to the prototype subfamily, whereas its carbohydrate-binding specificity is related to that of the mammalian chimera-type galectin-3. This dual coordination reveals GCG as a potential precursor of the later evolved galectin subfamilies, which is reflected in the primary structure of the protein. This study provides evidence that GCG is the LECT1 gene product, while neither a previously described LECT2 gene nor a functional LECT2 gene product was found in the specimen under investigation. The electrophoretically separated protein isomers with apparent molecular masses of 13, 15, and 16 kDa correspond to variants of the LECT1 protein-exhibiting peptide sequence polymorphisms that concern critical positions of the carbohydrate recognition domain (13 kDa: Leu51, Asn55, His130, Gly137; 15 kDa: Ser51, Asn55, Asn130, Gly137; 16 kDa: Ser51, Tyr55, Asn130, Glu137). Four residues, highly conserved in the galectin family, are substituted. None of the residues claimed to be involved in interactions with GalNAc alpha 1-3 moieties at an extended binding subsite of galectin-3 was identified in the corresponding positions of GCG. Apparently, the substitutions do not confer distinct binding characteristics to the GCG variants as evidenced by binding studies with a recombinantly expressed 15-kDa isoform. The natural isoforms as well as the recombinant 15-kDa isoform oligomerize by the formation of non-covalent heteromeric or homomeric complexes. A phosphorylation of the galectin was confirmed neither by mass spectrometry nor by alkaline phosphatase treatment combined with isoelectric focusing},
  language  = {en}
}
@article{SatoGambaleDreyeretal.2006,
  author    = {Sato, A and Gambale, Franco and Dreyer, Ingo and Uozumi, N},
  title     = {Posttranslational inodification affects K+ current of plant K+ channel},
  year      = {2006},
  language  = {en}
}
@article{SchulzThierbachVoigtetal.2006,
  author    = {Schulz, Tim Julius and Thierbach, Ren{\`e} and Voigt, Anja and Drewes, Gunnar and Mietzner, Brun and Steinberg, Pablo and Pfeiffer, Andreas F. H. and Ristow, Michael},
  title     = {Induction of oxidative metabolism by mitochondrial frataxin inhibits cancer growth : Otto Warburg revisited},
  doi       = {10.1074/jbc.M511064200},
  year      = {2006},
  abstract  = {More than 80 years ago Otto Warburg suggested that cancer might be caused by a decrease in mitochondrial energy metabolism paralleled by an increase in glycolytic flux. In later years, it was shown that cancer cells exhibit multiple alterations in mitochondrial content, structure, function, and activity. We have stably overexpressed the Friedreich ataxia-associated protein frataxin in several colon cancer cell lines. These cells have increased oxidative metabolism, as shown by concurrent increases in aconitase activity, mitochondrial membrane potential, cellular respiration, and ATP content. Consistent with Warburg's hypothesis, we found that frataxin-overexpressing cells also have decreased growth rates and increased population doubling times, show inhibited colony formation capacity in soft agar assays, and exhibit a reduced capacity for tumor formation when injected into nude mice. Furthermore, overexpression of frataxin leads to an increased phosphorylation of the tumor suppressor p38 mitogen-activated protein kinase, as well as decreased phosphorylation of extracellular signal-regulated kinase. Taken together, these results support the view that an increase in oxidative metabolism induced by mitochondrial frataxin may inhibit cancer growth in mammals},
  language  = {en}
}
@article{WronskiApioWankeretal.2006,
  author    = {Wronski, Torsten and Apio, Ann and Wanker, R and Plath, Martin},
  title     = {Behavioural repertoire of the bushbuck (Tragelaphus scriptus): agonistic interactions, mating behaviour and parent-offspring relations},
  doi       = {10.1007/s10164-005-0186-y},
  year      = {2006},
  abstract  = {A free-ranging bushbuck (Tragelaphus scriptus) population was observed over a period of 3 years, thereby enabling a detailed description of the behavioural repertoire of this widespread but barely investigated solitary African antelope species. Agonistic and submissive behaviour patterns are described, among them several hitherto un- described behaviour patterns - such as "escorting", where territorial males guide intruders to the periphery of their territory - and "push-up position", an extreme form of submissive behaviour. Furthermore, we report on behaviour patterns of males and females during mating as well as on behaviour patterns of parents directed towards their offspring. Again, we describe a hitherto unknown behaviour: the protection of calves by adult males, which may be a socio-positive behaviour directed towards their offspring led by kin selection},
  language  = {en}
}
@article{YuryevKascheIgnatovaetal.2010,
  author    = {Yuryev, Ruslan and Kasche, Volker and Ignatova, Zoya and Galunsky, Boris},
  title     = {Improved A. faecalis penicillin amidase mutant retains the thermodynamic and pH stability of the wild type enzyme},
  issn      = {1572-3887},
  doi       = {10.1007/s10930-010-9238-4},
  year      = {2010},
  abstract  = {Penicillin amidase from Alacaligenes faecalis is an attractive biocatalyst for hydrolysis of penicillin G for production of 6-aminopenicillanic acid, which is used in the synthesis of semi-synthetic beta-lactam antibiotics. Recently a mutant of this enzyme with extended C-terminus of the A-chain comprising parts of the connecting linker peptide was constructed. Its turnover number for the hydrolysis of penicillin G was 140 s(-1), about twice of the value for the wild-type enzyme (80 s(-1)). At the same time the specificity constant was improved about three-fold. The wild- type and the mutant enzymes showed similar pH stability suggesting that the linker peptide fragment covalently attached to the A-chain does not alter the electrostatic interactions in the protein core. Although the global stability of A. faecalis wild-type enzyme and the T206GS213G variant does not differ, the presence of the linker fragment stabilizes the domains interface, as evidenced by the monophasic transition of the mutant enzyme from folded to unfolded state during urea-induced denaturation. The high stability and activity of the mutant enzyme provides a rationale to use it as a biocatalyst in the industrial processes, where the enzyme must be more robust to fluctuations in the operational conditions.},
  language  = {en}
}
@article{LiuWollenbergerKatterleetal.2006,
  author    = {Liu, Songqin and Wollenberger, Ursula and Katterle, Martin and Scheller, Frieder W.},
  title     = {Ferroceneboronic acid-based amperometric biosensor for glycated hemoglobin},
  issn      = {0925-4005},
  doi       = {10.1016/j.snb.2005.07.011},
  year      = {2006},
  abstract  = {An amperometric biosensor for the determination of glycated hemoglobin in human whole blood is proposed. The principle is based on the electrochemical measurement of ferroceneboronic acid (FcBA) that has been specifically bound to the glycated N-terminus. Hemoglobin is immobilized on a zirconium dioxide nanoparticle modified pyrolytic graphite electrode (PGE) in the presence of didodecyldimethylammonium bromide (DDAB). The incubation of this sensor in FcBA solution leads to the formation of an FcBA-modified surface due to the affinity interaction between boronate and the glycated sites of the hemoglobin. The binding of FcBA results in well-defined redox peaks with an E-0' of 0.299 V versus Ag/AgCl (1 M KCl). The square wave voltammetric response of the bound FcBA reflects the amount of glycated hemoglobin at the surface. This signal increases linearily with the degree of glycated hemoglobin from 6.8 to 14.0\% of total immobilized hemoglobin. The scheme was applied to the determination of the fraction of glycated hemoglobin in whole blood samples.},
  language  = {en}
}
@article{ReinekingSchroederEsselbach2006,
  author    = {Reineking, Bj{\"o}rn and Schr{\"o}der-Esselbach, Boris},
  title     = {Constrain to perform : regularization of habitat models},
  issn      = {0304-3800},
  doi       = {10.1016/j.ecolmodel.2005.10.003},
  year      = {2006},
  abstract  = {Predictive habitat models are an important tool for ecological research and conservation. A major cause of unreliable models is excessive model complexity, and regularization methods aim to improve the predictive performance by adequately constraining model complexity. We compare three regularization methods for logistic regression: variable selection, lasso, and ridge. They differ in the way model complexity is measured: variable selection uses the number of estimated parameters, the lasso uses the sum of the absolute values of the parameter estimates, and the ridge uses the sum of the squared values of the parameter estimates. We performed a simulation study with environmental data of a real landscape and artificial species occupancy data. We investigated the effect of three factors on relative model performance: (1) the number of parameters (16, 10, 6, 2) in the 'true' model that determined the distribution of the artificial species, (2) the prevalence, i.e. the proportion of sites occupied by the species, and (3) the sample size (measured in events per variable, EPV). Regularization improved model discrimination and calibration. However, no regularization method performed best under all circumstances: the ridge generally performed best in the 16-parameter scenario. The lasso generally performed best in the 10-parameter scenario. Variable selection with AIC was best at large sample sizes (EPV >= 10) when less than half of the variables influenced the species distribution. However, at low sample sizes (EPV < 10), ridge and lasso always performed best, regardless of the parameter scenario or prevalence. Overall, calibration was best in ridge models. Other methods showed overconfidence, particularly at low sample sizes. The percentage of correctly identified models was low for both lasso and variable selection. Variable selection should be used with caution. Although it can produce the best performing models under certain conditions, these situations are difficult to infer from the data. Ridge and lasso are risk-averse model strategies that can be expected to perform well under a wide range of underlying species-habitat relationships, particularly at small sample sizes.},
  language  = {en}
}
@article{RossmanithGrimmBlaumetal.2006,
  author    = {Rossmanith, Eva and Grimm, Volker and Blaum, Niels and Jeltsch, Florian},
  title     = {Behavioural flexibility in the mating system buffers population extinction: lessons from the Lesser Spotted Woodpecker (Picoides minor)},
  doi       = {10.1111/j.1365-2656.2006.01074.x},
  year      = {2006},
  abstract  = {In most stochastic models addressing the persistence of small populations, environmental noise is included by imposing a synchronized effect of the environment on all individuals. However, buffer mechanisms are likely to exist that may counteract this synchronization to some degree. We have studied whether the flexibility in the mating system, which has been observed in some bird species, is a potential mechanism counteracting the synchronization of environmental fluctuations. Our study organism is the lesser spotted woodpecker Picoides minor (Linnaeus), a generally monogamous species. However, facultative polyandry, where one female mates with two males with separate nests, was observed in years with male-biased sex ratio. We constructed an individual-based model from data and observations of a population in Taunus, Germany. We tested the impact of three behavioural scenarios on population persistence: (1) strict monogamy; (2) polyandry without costs; and (3) polyandry assuming costs in terms of lower survival and reproductive success for secondary males. We assumed that polyandry occurs only in years with male-biased sex ratio and only for females with favourable breeding conditions. Even low rates of polyandry had a strong positive effect on population persistence. The increase of persistence with carrying capacity was slower in the monogamous scenario, indicating strong environmental noise. In the polyandrous scenarios, the increase of persistence was stronger, indicating a buffer mechanism. In the polyandrous scenarios, populations had a higher mean population size, a lower variation in number of individuals, and recovered faster after a population breakdown. Presuming a realistic polyandry rate and costs for polyandry, there was still a strong effect of polyandry on persistence. The results show that polyandry and in general flexibility in mating systems is a buffer mechanism that can significantly reduce the impact of environmental and demographic noise in small populations. Consequently, we suggest that even behaviour that seems to be exceptional should be considered explicitly when predicting the persistence of populations},
  language  = {en}
}
@article{BuscagliaSchulerLapidusetal.2003,
  author    = {Buscaglia, Marco and Schuler, Benjamin and Lapidus, Lisa J. and Eaton, Wiliam A. and Hofrichter, James},
  title     = {Kinetics of intramolecular contact formation in a denatured protein},
  issn      = {0022-2836},
  year      = {2003},
  abstract  = {Quenching of the triplet state of tryptophan by cysteine has provided a new tool for measuring the rate of forming a specific intramolecular contact in disordered polypeptides. Here, we use this technique to investigate contact formation in the denatured state of CspTm, a small cold-shock protein from Thermotoga maritima, engineered to contain a single tryptophan residue (W29) and a single cysteine residue at the C terminus (C67). At all concentrations of denaturant, the decay rate of the W29 triplet of the unfolded protein is more than tenfold faster than the rate observed for the native protein (not, vert, similar104 s;1). Experiments on the unfolded protein without the added C- terminal cysteine residue show that this faster rate results entirely from contact quenching by C67. The quenching rate in the unfolded state by C67 increases at concentrations of denaturant that favor folding, indicating a compaction of the unfolded protein as observed previously in single-molecule Foerster resonance energy transfer (FRET) experiments.},
  language  = {en}
}
@phdthesis{Kopka2006,
  author    = {Kopka, Claudia},
  title     = {The nucleoside diphosphate kinases - a small gene family involved in the energyphosphotransfer of arabidopsis thaliana},
  address   = {Potsdam},
  pages     = {133 Bl. : graph. Darst.},
  year      = {2006},
  language  = {en}
}
@phdthesis{Vakeel2006,
  author    = {Vakeel, Padmanabhan},
  title     = {Biochemical and cellular characterization of filamin binding proteins in cross striated muscle},
  address   = {Potsdam},
  pages     = {XV, 156 Bl. : graph. Darst.},
  year      = {2006},
  language  = {en}
}
@book{Koechy2006,
  author    = {K{\"o}chy, Martin},
  title     = {Opposite trends in life stages of annual plants caused by daily rainfall variability - interaction with climate change},
  year      = {2006},
  abstract  = {Global Circulation Models of climate predict not only a change of annual precipitation amounts but also a shift in the daily distribution. To improve the understanding of the importance of daily rain pattern for annual plant communities, which represent a large portion of semi-natural vegetation in the Middle East, I used a detailed, spatially explicit model. The model explicitly considers water storage in the soil and has been parameterized and validated with data collected in field experiments in Israel and data from the literature. I manipulated daily rainfall variability by increasing the mean daily rain intensity on rainy days (MDI, rain volume/day) and decreasing intervals between rainy days while keeping the mean annual amount constant. In factorial combination, I also increased mean annual precipitation (MAP). I considered five climatic regions characterized by 100, 300, 450, 600, and 800 mm MAP. Increasing MDI decreased establishment when MAP was >250 mm but increased establishment at more arid sites. The negative effect of increasing MDI was compensated by increasing mortality with increasing MDI in dry and typical Mediterranean regions (c. 360-720 mm MAP). These effects were strongly tied to water availability in upper and lower soil layers and modified by competition among seedlings and adults. Increasing MAP generally increased water availability, establishment, and density. The order of magnitudes of MDI and MAP effects overlapped partially so that their combined effect is important for projections of climate change effects on annual vegetation. The effect size of MAP and MDI followed a sigmoid curve along the MAP gradient indicating that the semi-arid region (?300 mm MAP) is the most sensitive to precipitation change with regard to annual communities.},
  language  = {en}
}
@phdthesis{Hett2006,
  author    = {Hett, Anne Kathrin},
  title     = {Evolution of nuclear DNA in sturgeons},
  address   = {Potsdam},
  pages     = {Getr. Z{\"a}hlung : graph. Darst.},
  year      = {2006},
  language  = {en}
}
@article{MoodleyBrufordBleidornetal.2009,
  author    = {Moodley, Yoshan and Bruford, Michael W. and Bleidorn, Christoph and Wronski, Torsten and Apio, Ann and Plath, Martin},
  title     = {Analysis of mitochondrial DNA data reveals non-monophyly in the bushbuck (Tragelaphus scriptus) complex},
  issn      = {1616-5047},
  doi       = {10.1016/j.mambio.2008.05.003},
  year      = {2009},
  language  = {en}
}
@article{NagelWarsinke2006,
  author    = {Nagel, Birgit and Warsinke, Axel},
  title     = {Towards separation-free electrochemical affinity sensors by using antibodies, aptamers and molecularly imprinted polymers : a review},
  doi       = {10.1080/00032710600853903},
  year      = {2006},
  language  = {en}
}
@article{NitscheKurthDunkhorstetal.2007,
  author    = {Nitsche, Andreas and Kurth, Andreas and Dunkhorst, Anna and P{\"a}nke, Oliver and Sielaff, Hendrik and Junge, Wolfgang and Muth, Doreen and Scheller, Frieder W. and St{\"o}cklein, Walter F. M. and Pauli, Georg and Kage, Andreas},
  title     = {One-step selection of vaccinia virus binding DNA-aptamers by MonoLEX},
  doi       = {10.1186/1472-6750-7-48},
  year      = {2007},
  language  = {en}
}
@article{FettkeChiaEckermannetal.2006,
  author    = {Fettke, J{\"o}rg and Chia, Tansy and Eckermann, Nora and Smith, Alison M. and Steup, Martin},
  title     = {A transglucosidase necessary for starch degradation and maltose metabolism in leaves at night acts on cytosolic heteroglycans (SHG)},
  issn      = {0960-7412},
  doi       = {10.1111/j.1365-313X.2006.02732.x},
  year      = {2006},
  abstract  = {The recently characterized cytosolic transglucosidase DPE2 (EC 2.4.1.25) is essential for the cytosolic metabolism of maltose, an intermediate on the pathway by which starch is converted to sucrose at night. In in vitro assays, the enzyme utilizes glycogen as a glucosyl acceptor but the in vivo acceptor molecules remained unknown. In this communication we present evidence that DPE2 acts on the recently identified cytosolic water-soluble heteroglycans (SHG) as does the cytosolic phosphorylase (EC 2.4.1.1) isoform. By using in vitro two-step C-14 labeling assays we demonstrate that the two transferases can utilize the same acceptor sites of the SHG. Cytosolic heteroglycans from a DPE2-deficient Arabidopsis mutant were characterized. Compared with the wild type the glucose content of the heteroglycans was increased. Most of the additional glucosyl residues were found in the outer chains of SHG that are released by an endo- alpha-arabinanase (EC 3.2.1.99). Additional starch-related mutants were characterized for further analysis of the increased glucosyl content. Based on these data, the cytosolic metabolism of starch-derived carbohydrates is discussed},
  language  = {en}
}
@article{KappBeissenhirtzGeyeretal.2006,
  author    = {Kapp, Andreas and Beissenhirtz, Moritz Karl and Geyer, F. and Scheller, Frieder W. and Viezzoli, Maria Silvia and Lisdat, Fred},
  title     = {Electrochemical and sensorial behaviour of SOD mutants immobilized on gold electrodes in aqueous / organic solvent mixtures},
  issn      = {1040-0397},
  doi       = {10.1002/elan.200603620},
  year      = {2006},
  language  = {en}
}
@article{Knoesche2006,
  author    = {Kn{\"o}sche, R{\"u}diger},
  title     = {Organic sediment nutrient concentrations and their relationship with the hydrological connectivity of floodplain waters (River Havel, NE Germany)},
  year      = {2006},
  abstract  = {Investigations on large canalised rivers, for example the Danube, have shown that transported particulate matter, which is typically inorganic, is predominantly deposited in waters near the river's main channel. This investigation deals with the lower section of the River Havel (NE Germany), a canalised lowland river with a very flat floodplain. This river is highly polluted by nutrients from urban areas (Berlin) and a long chain of river lakes produces high concentrations of phytoplankton. Due to the high proportion of planktogenic detritus, it was hypothesised that greater quantities of nutrient-rich fine particulate organic matter (FPOM) would be deposited in floodplain waters located further from the main channel than has been reported for large rivers.The total nutrient, P-binding metal (Fe, Al, and Mn), organic and inorganic carbon (TOC, TIC) contents of the upper organic sediment layer (0 - 4 cm) were analysed in samples collected from 48 floodplain water and river sites. The sediment bulk density, calculated on the basis of dry mass content and loss on ignition, was used to characterize the waters according to the impact of the river current. The results showed that the variability of total phosphorus (TP) was best explained by the variability of total iron (TFe, R2 = 0.52). The floodplain water sediments could clearly be separated into two groups on the basis of the sediment particle size composition, and of the element ratios TOC:TP, TN:TP, primarily TFe:TP. The sediments from impounded river sections and from mouth sections of backwaters (approx. 100 - 200 m) were characterized by a high proportion particles from the 0.1 - 0.5 mm size fraction and by homogeneous, low TFe:TP, TOC:TP and TN:TP ratios. Sediments from distal sections of backwaters and of oxbow lakes tended to exhibit high element ratios with much higher variability. These results were interpreted as a spatially limited impact of the river on the floodplain water sediments. Contrary to expectation, the phosphorus bound in river seston was predominantly and very homogeneously deposited in the impounded river and mouth sections of backwaters. This implies that the inundation of the floodplain waters during spring floods seems to have no important material impact on the sediments in waters of low hydrologically connectivity with the River Havel.},
  language  = {en}
}
@article{Micheel2006,
  author    = {Micheel, Burkhard},
  title     = {Monoclonal Antibodies},
  isbn      = {978-3-540-44244-8},
  year      = {2006},
  language  = {en}
}
@phdthesis{Caldana2007,
  author    = {Caldana, Camila},
  title     = {Genome wide identification and functional characterization of transcription factors involvend in the initial phase of salt stress in rice},
  address   = {Potsdam},
  pages     = {154 S., i-vi, : graph. Darst.},
  year      = {2007},
  language  = {en}
}
@phdthesis{Caldana2007,
  author    = {Caldana, Camila},
  title     = {Genome wide identification and functional characterization of transcription factors involved in the initial phase of salt stress in rice},
  address   = {Potsdam},
  pages     = {vi, 154 S.: Ill., graph. Darst.},
  year      = {2007},
  language  = {en}
}
@phdthesis{Nest2007,
  author    = {Nest, Mathias},
  title     = {Quantum dynamics for large systems : system-bath type situations and correlated dynamics of many electrons},
  address   = {Potsdam},
  pages     = {v, 176 S.: graph. Darst.},
  year      = {2007},
  language  = {en}
}
@phdthesis{Krueger2007,
  author    = {Kr{\"u}ger, Stephan},
  title     = {Studies on the compartmentation of cysteine biosynthesis},
  address   = {Potsdam},
  pages     = {133 Bl. : Ill., graph. Darst.},
  year      = {2007},
  language  = {en}
}
@phdthesis{Miyashita2007,
  author    = {Miyashita, Naoko},
  title     = {Molecular assemblies on surfaces},
  address   = {Potsdam},
  pages     = {viii, 86 S. : Ill., graph. Darst.},
  year      = {2007},
  language  = {en}
}
@phdthesis{Ratzka2007,
  author    = {Ratzka, Antje},
  title     = {Cell wall biosynthesis and metabolism in tomato fruit},
  address   = {Potsdam},
  pages     = {VII, 88 S. : graph. Darst.},
  year      = {2007},
  language  = {en}
}
@phdthesis{Redestig2007,
  author    = {Redestig, Henning},
  title     = {Approaches for integrative analysis of gene expression data},
  address   = {Potsdam},
  pages     = {VIII, 130 S. : Ill., graph. Darst.},
  year      = {2007},
  language  = {en}
}
@phdthesis{Bischoff2007,
  author    = {Bischoff, Volker},
  title     = {Molecular analysis of cellulose synthesis in Arabidopsis thaliana},
  address   = {Potsdam},
  pages     = {XIII, 113 S. : Ill, graph. Darst.},
  year      = {2007},
  language  = {en}
}
@phdthesis{Lehmann2007,
  author    = {Lehmann, Ute},
  title     = {Functional studies on sucrose-phosphate synthase in Arabidopsis thaliana},
  address   = {Potsdam},
  pages     = {VII, 96 S., XLVI : graph. Darst.},
  year      = {2007},
  language  = {en}
}
@phdthesis{Wasiolka2007,
  author    = {Wasiolka, Bernd},
  title     = {The impact of overgrazing on reptile diversity and population dynamics of Pedioplanis l. lineoocellata in the southern Kalahari},
  publisher = {Univ.-Verl.},
  address   = {Potsdam},
  pages     = {v, 101 BL. : Ill., graph. Darst.},
  year      = {2007},
  language  = {en}
}
@article{Spijkerman2007,
  author    = {Spijkerman, Elly},
  title     = {Is there really insufficient support for Tilman's R* concept? A comment on Miller et al},
  year      = {2007},
  language  = {en}
}
@article{Gaedke2007,
  author    = {Gaedke, Ursula},
  title     = {Spectral analysis unmasks synchronous and compensatory dynamics in plankton communities},
  year      = {2007},
  language  = {en}
}
@article{BellParkPearceetal.2007,
  author    = {Bell, Elanor M. and Park, Tae-Gyu and Pearce, Imojen and Rublee, Parke A. and Bolch, Christopher J. S. and Hallegraeff, Gustaff M.},
  title     = {Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR},
  year      = {2007},
  language  = {en}
}
@article{HigginsFloresSchurr2008,
  author    = {Higgins, Steven I. and Flores, Olivier and Schurr, Frank Martin},
  title     = {Costs of persistence and the spread of competing seeders and sprouters},
  issn      = {0022-0477},
  doi       = {10.1111/j.1365-2745.2008.01391.x},
  year      = {2008},
  language  = {en}
}
@article{Clegg2007,
  author    = {Clegg, Mark R.},
  title     = {Behavioral response as a predictor of seasonal depth distribution and vertical niche separation in freshwater phytoplanktonic flagellates},
  year      = {2007},
  language  = {en}
}
@article{DreyerGajdanowicz2009,
  author    = {Dreyer, Ingo and Gajdanowicz, Pawel},
  title     = {Regulation of the gating mode of the Arabidopsis K+ channel AKT2 is important for adaptation to abiotic stress},
  issn      = {1095-6433},
  doi       = {10.1016/j.cbpa.2009.04.426},
  year      = {2009},
  language  = {en}
}
@article{Spijkerman2007,
  author    = {Spijkerman, Elly},
  title     = {Phosphorus acquisition by Chlamydomonas acidophila under autotrophic and osmo-mixotrophic growth conditions},
  year      = {2007},
  language  = {en}
}
@article{ScherberEisenhauerWeisseretal.2010,
  author    = {Scherber, Christoph and Eisenhauer, Nico and Weisser, Wolfgang W. and Schmid, Bernhard and Voigt, Winfried and Fischer, Markus and Schukze, Ernst-Detlef and Roscher, Christiane and Weigelt, Alexandra and Allan, Eric and Beßler, Holger and Bonkowski, Michael and Buchmann, Nina and Buscot, Fran{\c{c}}ois and Clement, Lars W. and Ebeling, Anne and Engels, Christof and Halle, Stefan and Kertscher, Ilona and Klein, Alexandra-Maria and Koller, Robert and K{\"o}nig, Stephan and Kowalski, Esther and Kummer, Volker and Kuu, Annely and Lange, Markus and Lauterbach, Dirk},
  title     = {Bottom-up effects of plant diversity on multitrophic interactions in a biodiversity experiment},
  issn      = {0028-0836},
  year      = {2010},
  language  = {en}
}
@article{SchiffersSchurrTielboergeretal.2008,
  author    = {Schiffers, Katja and Schurr, Frank Martin and Tielb{\"o}rger, Katja and Urbach, Carsten and Moloney, Kirk A. and Jeltsch, Florian},
  title     = {Dealing with virtual aggregation : a new index for analysing heterogeneous point patterns},
  issn      = {0906-7590},
  doi       = {10.1111/j.0906-7590.2008.05374.x},
  year      = {2008},
  language  = {en}
}
@article{LoksteinHoextermannLeupoldetal.2009,
  author    = {Lokstein, Heiko and Hoextermann, Ekkehard and Leupold, Dieter and Garab, Gyoezoe and Renger, Gernot},
  title     = {A tribute : Professor Dr. Paul Hoffmann (March 28, 1931-July 10, 2008), a scientist with a great collaborative spirit},
  issn      = {0166-8595},
  doi       = {10.1007/s11120-009-9414-6},
  year      = {2009},
  language  = {en}
}
@article{SellrieSchenkBehrsingetal.2007,
  author    = {Sellrie, Frank and Schenk, J{\"o}rg A. and Behrsing, Olaf and Drechsel, Oliver and Micheel, Burkhard},
  title     = {Cloning and characterization of a single chain antibody to glucose oxidase from a murine hybridoma},
  year      = {2007},
  abstract  = {Glucose oxidase (GOD) is an oxidoreductase catalyzing the reaction of glucose and oxygen to peroxide and gluconolacton (EC 1.1.3.4.). GOD is a widely used enzyme in biotechnology. Therefore the production of monoclonal antibodies and antibody fragments to GOD are of interest in bioanalytics and even tumor therapy. We describe here the generation of a panel of monoclonal antibodies to native and heat inactivated GOD. One of the hybridomas, E13BC8, was used for cloning of a single chain antibody (scFv). This scFv was expressed in Escherichia coli XL1-blue with the help of the vector system pOPE101. The scFv was isolated from the periplasmic fraction and detected by western blotting. It reacts specifically with soluble active GOD but does not recognize denatured GOD adsorbed to the solid phase. The same binding properties were also found for the monoclonal antibody E13BC8.},
  language  = {en}
}
@article{SchenkSellrieBoettgeretal.2007,
  author    = {Schenk, J{\"o}rg A. and Sellrie, Frank and B{\"o}ttger, Volker and Micheel, Burkhard and St{\"o}cklein, Walter F. M.},
  title     = {Generation and application of a fluorescein-specific single chain antibody},
  year      = {2007},
  abstract  = {A recombinant single chain antibody fragment (designated scDE1) of the murine monoclonal anti-fluorescein antibody B13-DE1 was generated using the original hybridoma cells as source for the variable antibody heavy and light chain (VH and VL) genes. After cloning the variable genes into a phage vector a functional antibody fragment was selected by phage display panning. Recombinant antibody could be expressed as phage antibody and as soluble single chain antibody in Escherichia coli. High yield of scDE1 could also be detected in bacterial culture supernatant. The scDE1 showed the same binding specificity as the parental monoclonal antibody, i.e. it bound fluorescein, fluorescein derivatives and a fluorescein peptide mimotope. Surface plasmon resonance revealed a K(D) of 19 nM for the scDE1 compared to 0.7 nM for the monoclonal antibody. The isolated soluble scDE1 could easily be conjugated to horseradish peroxidase which allowed the use of the conjugate as universal indicator for the detection of fluorescein-labelled proteins in different immunoassays. Detection of hCG in urine was performed as a model system using scDE1. In addition to E. coli the scFv genes could also be transferred and expressed in eukaryotic cells. Finally, we generated HEK293 cells expressing the scDE1 at the cell surface.},
  language  = {en}
}
@article{LawatscheckAleksaiteSchenketal.2007,
  author    = {Lawatscheck, Robert and Aleksaite, Egle and Schenk, J{\"o}rg A. and Micheel, Burkhard and Jandrig, Burkhard and Holland, Gudrun and Sasnauskas, Kestutius and Gedvilaite, Alma and Ulrich, Rainer G{\"u}nter},
  title     = {Chimeric polyomavirus-derived virus-like particles : the immunogenicity of an inserted peptide applied without adjuvant to mice depends on its insertion site and its flanking linker sequence},
  issn      = {0882-8245},
  doi       = {10.1089/vim.2007.0023},
  year      = {2007},
  abstract  = {We inserted the sequence of the carcinoembryonic antigen-derived T cell epitope CAP-1-6D (CEA) into different positions of the hamster polyomavirus major capsid protein VP1. Independently from additional flanking linkers, yeast- expressed VP1 proteins harboring the CEA insertion between VP1 amino acid residues 80 and 89 (site 1) or 288 and 295 (site 4) or simultaneously at both positions assembled to chimeric virus-like particles (VLPs). BALB/c mice immunized with adjuvant-free VLPs developed VP1- and epitope-specific antibodies. The level of the CEA-specific antibody response was determined by the insertion site, the number of inserts, and the flanking linker. The strongest CEA-specific antibody response was observed in mice immunized with VP1 proteins harboring the CEA insert at site 1. Moreover, the CEA- specific antibodies in these mice were still detectable 6 mo after the final booster immunization. Our results indicate that hamster polyomavirus-derived VLPs represent a highly immunogenic carrier for foreign insertions that might be useful for clinical and therapeutic applications.},
  language  = {en}
}