@article{GronauBenderBertheauetal.2021,
  author    = {Gronau, Norbert and Bender, Benedict and Bertheau, Clementine and Lauppe, Hannah},
  title     = {Robotic Process Automation statt neuem ERP-System},
  series = {ERP-Management : Auswahl, Einf{\"u}hrung und Betrieb von ERP-Systemen},
  volume    = {17},
  journal   = {ERP-Management : Auswahl, Einf{\"u}hrung und Betrieb von ERP-Systemen},
  number    = {1},
  publisher = {GITO mbH Verlag},
  address   = {Berlin},
  issn      = {1860-6725},
  doi       = {10.30844/ERP_21-1_29-32},
  pages     = {29 -- 32},
  year      = {2021},
  abstract  = {Robotic Process Automation (RPA) steht f{\"u}r die softwareunterst{\"u}tzte Bedienung von Softwarel{\"o}sungen {\"u}ber deren Benutzeroberfl{\"a}che. Das prim{\"a}re Ziel, das mit RPA erreicht werden soll, ist die automatisierte Ausf{\"u}hrung von Routineaufgaben, die bisher einen menschlichen Eingriff erforderten. Das Potenzial von RPA, Prozesse langfristig zu verbessern, ist allerdings stark begrenzt. Die Automatisierung von Prozessen und die {\"U}berbr{\"u}ckung von Medienbr{\"u}chen auf der Front-End-Ebene f{\"u}hrt zu einer Vielzahl von Abh{\"a}ngigkeiten und Bedingungen, die in diesem Beitrag zusammengefasst werden. Der Weg zu einer nachhaltigen Unternehmensarchitektur (bestehend aus Prozessen und Systemen) erfordert offene, adaptive Systeme mit moderner Architektur, die sich durch ein hohes Maß an Interoperabilit{\"a}t auf verschiedenen Ebenen auszeichnen.},
  language  = {de}
}
@article{KerstingRauschBieretal.2014,
  author    = {Kersting, Sebastian and Rausch, Valentina and Bier, Frank Fabian and von Nickisch-Rosenegk, Markus},
  title     = {Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis},
  series = {Malaria journal},
  volume    = {13},
  journal   = {Malaria journal},
  publisher = {BioMed Central},
  address   = {London},
  issn      = {1475-2875},
  doi       = {10.1186/1475-2875-13-99},
  pages     = {9},
  year      = {2014},
  abstract  = {Background: Nucleic acid amplification is the most sensitive and specific method to detect Plasmodium falciparum. However the polymerase chain reaction remains laboratory-based and has to be conducted by trained personnel. Furthermore, the power dependency for the thermocycling process and the costly equipment necessary for the read-out are difficult to cover in resource-limited settings. This study aims to develop and evaluate a combination of isothermal nucleic acid amplification and simple lateral flow dipstick detection of the malaria parasite for point-of-care testing. Methods: A specific fragment of the 18S rRNA gene of P. falciparum was amplified in 10 min at a constant 38 C using the isothermal recombinase polymerase amplification (RPA) method. With a unique probe system added to the reaction solution, the amplification product can be visualized on a simple lateral flow strip without further labelling. The combination of these methods was tested for sensitivity and specificity with various Plasmodium and other protozoa/bacterial strains, as well as with human DNA. Additional investigations were conducted to analyse the temperature optimum, reaction speed and robustness of this assay. Results: The lateral flow RPA (LF-RPA) assay exhibited a high sensitivity and specificity. Experiments confirmed a detection limit as low as 100 fg of genomic P. falciparum DNA, corresponding to a sensitivity of approximately four parasites per reaction. All investigated P. falciparum strains (n = 77) were positively tested while all of the total 11 non-Plasmodium samples, showed a negative test result. The enzymatic reaction can be conducted under a broad range of conditions from 30-45 degrees C with high inhibitory concentration of known PCR inhibitors. A time to result of 15 min from start of the reaction to read-out was determined. Conclusions: Combining the isothermal RPA and the lateral flow detection is an approach to improve molecular diagnostic for P. falciparum in resource-limited settings. The system requires none or only little instrumentation for the nucleic acid amplification reaction and the read-out is possible with the naked eye. Showing the same sensitivity and specificity as comparable diagnostic methods but simultaneously increasing reaction speed and dramatically reducing assay requirements, the method has potential to become a true point-of-care test for the malaria parasite.},
  language  = {en}
}
@article{KerstingRauschBieretal.2014,
  author    = {Kersting, Sebastian and Rausch, Valentina and Bier, Frank Fabian and von Nickisch-Rosenegk, Markus},
  title     = {Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens},
  series = {Microchimica acta : analytical sciences based on micro- and nanomaterials},
  volume    = {181},
  journal   = {Microchimica acta : analytical sciences based on micro- and nanomaterials},
  number    = {13-14},
  publisher = {Springer},
  address   = {Wien},
  issn      = {0026-3672},
  doi       = {10.1007/s00604-014-1198-5},
  pages     = {1715 -- 1723},
  year      = {2014},
  abstract  = {We report on the development of an on-chip RPA (recombinase polymerase amplification) with simultaneous multiplex isothermal amplification and detection on a solid surface. The isothermal RPA was applied to amplify specific target sequences from the pathogens Neisseria gonorrhoeae, Salmonella enterica and methicillin-resistant Staphylococcus aureus (MRSA) using genomic DNA. Additionally, a positive plasmid control was established as an internal control. The four targets were amplified simultaneously in a quadruplex reaction. The amplicon is labeled during on-chip RPA by reverse oligonucleotide primers coupled to a fluorophore. Both amplification and spatially resolved signal generation take place on immobilized forward primers bount to expoxy-silanized glass surfaces in a pump-driven hybridization chamber. The combination of microarray technology and sensitive isothermal nucleic acid amplification at 38 degrees C allows for a multiparameter analysis on a rather small area. The on-chip RPA was characterized in terms of reaction time, sensitivity and inhibitory conditions. A successful enzymatic reaction is completed in < 20 min and results in detection limits of 10 colony-forming units for methicillin-resistant Staphylococcus aureus and Salmonella enterica and 100 colony-forming units for Neisseria gonorrhoeae. The results show this method to be useful with respect to point-of-care testing and to enable simplified and miniaturized nucleic acid-based diagnostics.},
  language  = {en}
}