@article{QuicletDittbernerGaessleretal.2019,
  author    = {Quiclet, Charline and Dittberner, Nicole and Gaessler, Anneke and Stadion, Mandy and Gerst, Felicia and Helms, Anett and Baumeier, Christian and Schulz, Tim Julius and Schurmann, Annette},
  title     = {Pancreatic adipocytes mediate hypersecretion of insulin in diabetes-susceptible mice},
  series = {Metabolism - Clinical and experimental},
  volume    = {97},
  journal   = {Metabolism - Clinical and experimental},
  publisher = {Elsevier},
  address   = {Philadelphia},
  issn      = {0026-0495},
  doi       = {10.1016/j.metabol.2019.05.005},
  pages     = {9 -- 17},
  year      = {2019},
  abstract  = {Objective: Ectopic fat accumulation in the pancreas in response to obesity and its implication on the onset of type 2 diabetes remain poorly understood. Intermittent fasting (IF) is known to improve glucose homeostasis and insulin resistance. However, the effects of IF on fat in the pancreas and beta-cell function remain largely unknown. Our aim was to evaluate the impact of IF on pancreatic fat accumulation and its effects on islet function. Methods: New Zealand Obese (NZO) mice were fed a high-fat diet ad libitum (NZO-AL) or fasted every other day (intermittent fasting, NZO-IF) and pancreatic fat accumulation, glucose homoeostasis, insulin sensitivity, and islet function were determined and compared to ad libitum-fed B6.V-Lep(ob/ob) (ob/ob) mice. To investigate the crosstalk of pancreatic adipocytes and islets, co-culture experiments were performed. Results: NZO-IF mice displayed better glucose homeostasis and lower fat accumulation in both the pancreas (-32\%) and the liver (-35\%) than NZO-AL mice. Ob/ob animals were insulin-resistant and had low fat in the pancreas but high fat in the liver. NZO-AL mice showed increased fat accumulation in both organs and exhibited an impaired islet function. Co-culture experiments demonstrated that pancreatic adipocytes induced a hypersecretion of insulin and released higher levels of free fatty adds than adipocytes of inguinal white adipose tissue. Conclusions: These results suggest that pancreatic fat participates in diabetes development, but can be prevented by IF. (C) 2019 Published by Elsevier Inc.},
  language  = {en}
}