@article{BartelHartmannLehmannetal.2012,
  author    = {Bartel, Manuela and Hartmann, Stefanie and Lehmann, Karola and Postel, Kai and Quesada, Humberto and Philipp, Eva E. R. and Heilmann, Katja and Micheel, Burkhard and Stuckas, Heiko},
  title     = {Identification of sperm proteins as candidate biomarkers for the analysis of reproductive isolation in Mytilus: a case study for the enkurin locus},
  series = {Marine biology : international journal on life in oceans and coastal waters},
  volume    = {159},
  journal   = {Marine biology : international journal on life in oceans and coastal waters},
  number    = {10},
  publisher = {Springer},
  address   = {New York},
  issn      = {0025-3162},
  doi       = {10.1007/s00227-012-2005-7},
  pages     = {2195 -- 2207},
  year      = {2012},
  abstract  = {Sperm proteins of the marine sessile mussels of the Mytilus edulis species complex are models to investigate reproductive isolation and speciation. This study aimed at identifying sperm proteins and their corresponding genes. This was aided by the use of monoclonal antibodies that preferentially bind to yet unknown sperm molecules. By identifying their target molecules, this approach identified proteins with relevance to Mytilus sperm function. This procedure identified 16 proteins, for example, enkurin, laminin, porin and heat shock proteins. The potential use of these proteins as genetic markers to study reproductive isolation is exemplified by analysing the enkurin locus. Enkurin evolution is driven by purifying selection, the locus displays high levels of intraspecific variation and species-specific alleles group in distinct phylogenetic clusters. These findings characterize enkurin as informative candidate biomarker for analyses of clinal variation and differential introgression in hybrid zones, for example, to understand determinants of reproductive isolation in Baltic Mytilus populations.},
  language  = {en}
}
@article{HartmannHelmNickeletal.2012,
  author    = {Hartmann, Stefanie and Helm, Conrad and Nickel, Birgit and Meyer, Matthias and Struck, Torsten H. and Tiedemann, Ralph and Selbig, Joachim and Bleidorn, Christoph},
  title     = {Exploiting gene families for phylogenomic analysis of myzostomid transcriptome data},
  series = {PLoS one},
  volume    = {7},
  journal   = {PLoS one},
  number    = {1},
  publisher = {PLoS},
  address   = {San Fransisco},
  issn      = {1932-6203},
  doi       = {10.1371/journal.pone.0029843},
  pages     = {8},
  year      = {2012},
  abstract  = {Background: In trying to understand the evolutionary relationships of organisms, the current flood of sequence data offers great opportunities, but also reveals new challenges with regard to data quality, the selection of data for subsequent analysis, and the automation of steps that were once done manually for single-gene analyses. Even though genome or transcriptome data is available for representatives of most bilaterian phyla, some enigmatic taxa still have an uncertain position in the animal tree of life. This is especially true for myzostomids, a group of symbiotic ( or parasitic) protostomes that are either placed with annelids or flatworms. Methodology: Based on similarity criteria, Illumina-based transcriptome sequences of one myzostomid were compared to protein sequences of one additional myzostomid and 29 reference metazoa and clustered into gene families. These families were then used to investigate the phylogenetic position of Myzostomida using different approaches: Alignments of 989 sequence families were concatenated, and the resulting superalignment was analyzed under a Maximum Likelihood criterion. We also used all 1,878 gene trees with at least one myzostomid sequence for a supertree approach: the individual gene trees were computed and then reconciled into a species tree using gene tree parsimony. Conclusions: Superalignments require strictly orthologous genes, and both the gene selection and the widely varying amount of data available for different taxa in our dataset may cause anomalous placements and low bootstrap support. In contrast, gene tree parsimony is designed to accommodate multilocus gene families and therefore allows a much more comprehensive data set to be analyzed. Results of this supertree approach showed a well-resolved phylogeny, in which myzostomids were part of the annelid radiation, and major bilaterian taxa were found to be monophyletic.},
  language  = {en}
}