@article{GarzSandmannRadingetal.2012,
  author    = {Garz, Andreas and Sandmann, Michael and Rading, Michael and Ramm, Sascha and Menzel, Ralf and Steup, Martin},
  title     = {Cell-to-cell diversity in a synchronized chlamydomonas culture as revealed by single-cell analyses},
  series = {Biophysical journal},
  volume    = {103},
  journal   = {Biophysical journal},
  number    = {5},
  publisher = {Cell Press},
  address   = {Cambridge},
  issn      = {0006-3495},
  doi       = {10.1016/j.bpj.2012.07.026},
  pages     = {1078 -- 1086},
  year      = {2012},
  abstract  = {In a synchronized photoautotrophic culture of Chlamydomonas reinhardtii, cell size, cell number, and the averaged starch content were determined throughout the light-dark cycle. For single-cell analyses, the relative cellular starch was quantified by measuring the second harmonic generation (SHG). In destained cells, amylopectin essentially represents the only biophotonic structure. As revealed by various validation procedures, SHG signal intensities are a reliable relative measure of the cellular starch content. During photosynthesis-driven starch biosynthesis, synchronized Chlamydomonas cells possess an unexpected cell-to-cell diversity both in size and starch content, but the starch-related heterogeneity largely exceeds that of size. The cellular volume, starch content, and amount of starch/cell volume obey lognormal distributions. Starch degradation was initiated by inhibiting the photosynthetic electron transport in illuminated cells or by darkening. Under both conditions, the averaged rate of starch degradation is almost constant, but it is higher in illuminated than in darkened cells. At the single-cell level, rates of starch degradation largely differ but are unrelated to the initial cellular starch content. A rate equation describing the cellular starch degradation},
  language  = {en}
}
@article{SandmannGarzMenzel2016,
  author    = {Sandmann, Michael and Garz, Andreas and Menzel, Ralf},
  title     = {Physiological response of two different Chlamydomonas reinhardtii strains to light-dark rhythms},
  series = {Botany},
  volume    = {94},
  journal   = {Botany},
  publisher = {NRC Research Press},
  address   = {Ottawa},
  issn      = {1916-2790},
  doi       = {10.1139/cjb-2015-0144},
  pages     = {53 -- 64},
  year      = {2016},
  abstract  = {Cells of a cell-wall deficient line (cw15-type) of Chlamydomonas reinhardtii and of the corresponding wild type were grown during repetitive light-dark cycles. In a direct comparison, both lines showed approximately the same relative biomass increase during light phase but the cw-line produced significantly more, and smaller, daughter cells. Throughout the light period the average cellular starch content, the cellular chlorophyll content, the cellular rate of dark respiration, and the cellular rate of photosynthesis of the cw-line was lower. Despite this, several non-cell volume related parameters like the development of starch content per cell volume were clearly different over time between the strains. Additionally, the chlorophyll-based photosynthesis rates were 2-fold higher in the mutant than in the wild-type cells, and the ratio of chlorophyll a to chlorophyll b as well as the light-saturation index were also consistently higher in the mutant cells. Differences in the starch content were also confirmed by single cell analyses using a sensitive SHG-based microscopy approach. In summary, the cw15-type mutant deviates from its genetic background in the entire cell physiology. Both lines should be used in further studies in comparative systems biology with focus on the detailed relation between cell volume increase, photosynthesis, starch metabolism, and daughter cell productivity.},
  language  = {en}
}