@article{PecherSpahnSchirrmannetal.2001,
  author    = {Pecher, Gabriele and Spahn, Gunter and Schirrmann, Thomas and Kulbe, Hagen and Ziegner, Maja and Schenk, J{\"o}rg A. and Sandig, Volker},
  title     = {Mucin gene (MUC1) transfer into human dendritic cells by cationic liposomes and recombinant adenovirus},
  issn      = {0250-7005},
  year      = {2001},
  abstract  = {BACKGROUND: Dendritic cells (DC) as antigen presenting cells play an important role in immunotherapy of cancer. Mucin, encoded by the gene MUC1, is a human tumor antigen expressed in breast, pancreatic and ovarian cancers. Therefore, MUC1-transfected DC would be an attractive tool in constructing cancer vaccines. MATERIALS AND METHODS: Using two different cationic liposome preparations and, for comparison, a recombinant adenovirus expressing mucin, we tested the efficiency of mucin gene transfer into DC by flow cytometry. We investigated if these transfected DC were able to specifically stimulate autologous peripheral blood lymphocytes (PBL) from healthy donors. RESULTS: Flow cytometry revealed that 5-20\% of DC transfected with liposomes Lipofectin and 20-40\% of DC transduced with adenovirus expressed the relevant mucin epitopes. The expression of mucin on DC was similar to the expression of mucin found on carcinoma cells. After antigen uptake, DC specifically stimulated autologous PBL. CONCLUSION: We have shown that cationic liposomal gene transfer into human DC was feasible. We could obtain antigen specific stimulation of PBL at a similar rate as with adenoviral MUC1-transduced DC.},
  language  = {en}
}
@article{PecherSchirrmannKaiseretal.2001,
  author    = {Pecher, Gabriele and Schirrmann, Thomas and Kaiser, Lothar and Schenk, J{\"o}rg A.},
  title     = {Efficient cryopreservation of dendritic cells transfected with cDNA of a tumour antigen for clinical application},
  year      = {2001},
  abstract  = {Dendritic cells (DCs) are the most potent antigen-presenting cells of the immune system and are currently being investigated in clinical applications as cancer vaccines. An efficient cryopreservation method would greatly contribute to their use in clinical trials. We have established a method for freezing of DCs derived from peripheral blood mononuclear cells using the plasma expander Gelifundol. This enabled us to reduce the concentration of the toxic DMSO to 5\%. The method could be performed without the addition of fetal calf serum or any other serum. After freezing, the viability of the DCs was 90\%. The cells exhibited all the phenotypic characteristics (CD11c+, HLA-DR+, CD80+, CD83+, CD86+) of DCs, as tested by flow cytometry. Cells transfected with cDNA for the tumour antigen mucin expressed this protein on their surfaces in the same manner as before freezing. The stimulating capacity of a mixed lymphocyte culture was also preserved. These findings offer an efficient method for the cryopreservation of DCs for use in clinical trials.},
  language  = {en}
}