@phdthesis{Kuhnert2012, author = {Kuhnert, Oliver}, title = {Charakterisierung der neuen centrosomalen Proteine CP148 und CP55 in Dictyostelium discoideum}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59949}, school = {Universit{\"a}t Potsdam}, year = {2012}, abstract = {Das im Cytosol liegende Dictyostelium Centrosom ist aus einer geschichteten Core-Region aufgebaut, die von einer Mikrotubuli-nukleierenden Corona umgeben ist. Zudem ist es {\"u}ber eine spezifische Verbindung eng an den Kern gekn{\"u}pft und durch die Kernmembran hindurch mit den geclusterten Centromeren verbunden. Beim G2/M {\"U}bergang dissoziiert die Corona vom Centrosom und der Core verdoppelt sich so dass zwei Spindelpole entstehen. CP55 und CP148 wurden in einer Proteom-Analyse des Centrosoms identifiziert. CP148 ist ein neues coiled-coil Protein der centrosomalen Corona. Es zeigt eine zellzyklusabh{\"a}ngige An- und Abwesenheit am Centrosom, die mit der Dissoziation der Corona in der Prophase und ihrer Neubildung in der Telophase korreliert. W{\"a}hrend der Telophase erschienen in GFP-CP148 exprimierenden Zellen viele, kleine GFP-CP148-Foci im Cytoplasma, die zum Teil miteinander fusionierten und zum Centrosom wanderten. Daraus resultierte eine hypertrophe Corona in Zellen mit starker GFP-CP148 {\"U}berexpression. Ein Knockdown von CP148 durch RNAi f{\"u}hrte zu einem Verlust der Corona und einem ungeordneten Interphase Mikrotubuli-Cytoskelett. Die Bildung der mitotischen Spindel und der astralen Mikrotubuli blieb davon unbeeinflusst. Das bedeutet, dass die Mikrotubuli-Nukleationskomplexe w{\"a}hrend der Interphase und Mitose {\"u}ber verschiedene Wege mit dem Core assoziiert sind. Des Weiteren bewirkte der Knockdown eine Dispersion der Centromere sowie eine ver{\"a}nderte Sun1 Lokalisation in der Kernh{\"u}lle. Somit spielt CP148 ebenso eine Rolle in der Centrosomen-Centromer-Verbindung. Zusammengefasst ist CP148 ein essentielles Protein f{\"u}r die Bildung und Organisation der Corona, welche wiederum f{\"u}r die Centrosom/Centromer Verbindung ben{\"o}tigt wird. CP55 wurde als Protein der Core-Region identifiziert und verbleibt w{\"a}hrend des Zellzyklus am Centrosom. Dort besitzt es strukturelle Aufgaben, da die Mehrheit der GFP-CP55 Molek{\"u}le in der Interphase keine Mobilit{\"a}t zeigten. Die GFP-CP55 {\"U}berexpression f{\"u}hrte zur Bildung von {\"u}berz{\"a}hligen Centrosomen mit der {\"u}blichen Ausstattung an Markerproteinen der Corona und des Cores. CP55 Knockout-Zellen waren durch eine erh{\"o}hte Ploidie, eine weniger strukturierte und leicht vergr{\"o}ßerte Corona sowie zus{\"a}tzliche cytosolische Mikrotubuli-organisierende Zentren charakterisiert. Letztere entstanden in der Telophase und enthielten nur Corona- aber keine Core-Proteine. In CP55 k/o Zellen erfolgte die Rekrutierung des Corona-Organisators CP148 an den Spindelpol bereits in der fr{\"u}hen Metaphase anstatt, wie {\"u}blich, erst in der Telophase. Außerdem zeigten die Knockout-Zellen Wachstumsdefekte, deren Grund vermutlich Schwierigkeiten bei der Centrosomenverdopplung in der Prophase durch das Fehlen von CP55 waren. Dar{\"u}ber hinaus konnten die Knockout-Zellen phagozytiertes Material nicht verwerten, obwohl der Vorgang der Phagozytose nicht beeintr{\"a}chtigt war. Dieser Defekt kann dem im CP55 k/o auftretenden dispergierten Golgi-Apparat zugeschrieben werden.}, language = {de} } @phdthesis{Mahr2012, author = {Mahr, Philipp}, title = {Resource efficient communication in network-based reconfigurable on-chip systems}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59914}, school = {Universit{\"a}t Potsdam}, year = {2012}, abstract = {The constantly growing capacity of reconfigurable devices allows simultaneous execution of complex applications on those devices. The mere diversity of applications deems it impossible to design an interconnection network matching the requirements of every possible application perfectly, leading to suboptimal performance in many cases. However, the architecture of the interconnection network is not the only aspect affecting performance of communication. The resource manager places applications on the device and therefore influences latency between communicating partners and overall network load. Communication protocols affect performance by introducing data and processing overhead putting higher load on the network and increasing resource demand. Approaching communication holistically not only considers the architecture of the interconnect, but communication-aware resource management, communication protocols and resource usage just as well. Incorporation of different parts of a reconfigurable system during design- and runtime and optimizing them with respect to communication demand results in more resource efficient communication. Extensive evaluation shows enhanced performance and flexibility, if communication on reconfigurable devices is regarded in a holistic fashion.}, language = {en} } @book{OPUS4-5773, title = {Klimapolitik International}, editor = {Kleinw{\"a}chter, Kai}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-173-8}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-58354}, publisher = {Universit{\"a}t Potsdam}, pages = {100}, year = {2012}, abstract = {Der Klimawandel birgt globale Risiken. Dies ist weithin anerkannt. Umstritten ist die Frage, wie diesen Gefahren zu begegnen ist. 14 Jahre nach Kyoto ist klar: Klimapolitik ist Interessenpolitik. Das zeigte zuletzt die UN-Klimakonferenz 2011 in Durban mit ihren unverbindlichen Ergebnissen. In diesem Sammelband analysieren Experten Ursachen f{\"u}r das Scheitern der vergangenen Klima-Konferenzen und Konsequenzen f{\"u}r die k{\"u}nftige Klimapolitik. Insbesondere geht es um die Politik der Akteure China und Indien, um die Rolle der NGOs und die Klimafrage in der Entwicklungspolitik. Zugleich wird die deutsche Klimapolitik diskutiert und die Politik Brandenburgs von der verantwortlichen Ministerin vorgestellt.}, language = {de} } @phdthesis{Krentz2012, author = {Krentz, Eva Maria}, title = {Essst{\"o}rungen bei Jugendlichen im Hochleistungssport : eine Analyse sportbezogener Einflussfaktoren}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59933}, school = {Universit{\"a}t Potsdam}, year = {2012}, abstract = {Essst{\"o}rungen, wie Anorexia Nervosa oder Bulimia Nervorsa, gehen mit einer hohen psychischen Belastung einher und k{\"o}nnen gesundheitliche Sch{\"a}den zur Folge haben. Bei Athleten mit einer Essst{\"o}rung kann es dar{\"u}ber hinaus zu Einbußen in der Sportleistung kommen. Gerade f{\"u}r den Hochleistungssport ist es daher wichtig zu wissen, welches Risiko f{\"u}r Essst{\"o}rungen besteht und wodurch das Risiko bedingt wird. Bisherige Studien zeigen deutliche Unterschiede zwischen den Sportarten. Eliteathleten aus {\"a}sthetischen Sportarten, wie rhythmische Sportgymnastik oder Eiskunstlauf, scheinen ein besonders hohes Essst{\"o}rungsrisiko aufzuweisen. Deutlich geringere Pr{\"a}valenzraten finden sich in Ballsportarten, wie Handball oder Basketball. Um zu verstehen, welche Aspekte der Sportart das Essst{\"o}rungsrisiko beeinflussen, besch{\"a}ftigt sich die vorliegende Arbeit mit der Rolle sportbezogener Variablen. In die Studien einbezogen wurden insgesamt 171 Athleten zwischen 11 und 18 Jahren (im Mittel 14.1 ± 1.8 Jahre) aus {\"a}sthetischen Sportarten und Ballsportarten, die einen Fragebogenpaket mit Instrumenten zu gest{\"o}rtem Essverhalten, allgemeiner K{\"o}rperunzufriedenheit, sozialem Druck im Sport, sportbezogener K{\"o}rperunzufriedenheit, Schlankheitsstreben zur Leistungssteigerung und negativen Gef{\"u}hle bei Trainingsausfall ausf{\"u}llten. Nach einem Jahr wieder befragt wurden 65 Athleten aus {\"a}sthetischen Sportarten. Nach Kontrolle von Alter, Geschlecht, BMI und allgemeiner K{\"o}rperunzufriedenheit trugen sportbezogene Variablen signifikant zur weiteren Varianzaufkl{\"a}rung gest{\"o}rten Essverhaltens bei. Die L{\"a}ngsschnittanalysen best{\"a}tigten einen Risikofaktorstatus f{\"u}r Schlankheitsstreben zur Leistungssteigerung. Zusammenh{\"a}nge zwischen sportbezogenen Aspekten und gest{\"o}rtem Essverhalten zeigten sich sowohl in Hochrisikosportarten f{\"u}r gest{\"o}rtes Essverhalten ({\"a}sthetischen Sportarten), als auch in Niedrigrisikosportarten f{\"u}r gest{\"o}rtes Essverhalten (Ballsportarten). Mit Ausnahme von negativen Gef{\"u}hlen nach Trainingsausfall traten die sportbezogenen Variabeln h{\"a}ufiger in den {\"a}sthetischen Sportarten auf als in den Ballsportarten. Die eigenen Befunde verdeutlichen somit, dass der Einbezug potentieller sportbezogener Risikofaktoren - zus{\"a}tzlich zu den allgemeinen Risikofaktoren - zum besseren Verst{\"a}ndnis der Essst{\"o}rungssymptomatik von Athleten beitr{\"a}gt. Vor allem die Bedeutung von Gewicht f{\"u}r die Leistung beeinflusst das Essst{\"o}rungsrisiko bei Athleten und ist st{\"a}rker ausgepr{\"a}gt in Hochrisikosportarten f{\"u}r Essst{\"o}rungssymptomatik.}, language = {mul} } @phdthesis{Miasnikova2012, author = {Miasnikova, Anna}, title = {New hydrogel forming thermo-responsive block copolymers of increasing structural complexity}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59953}, school = {Universit{\"a}t Potsdam}, year = {2012}, abstract = {This work describes the synthesis and characterization of stimuli-responsive polymers made by reversible addition-fragmentation chain transfer (RAFT) polymerization and the investigation of their self-assembly into "smart" hydrogels. In particular the hydrogels were designed to swell at low temperature and could be reversibly switched to a collapsed hydrophobic state by rising the temperature. Starting from two constituents, a short permanently hydrophobic polystyrene (PS) block and a thermo-responsive poly(methoxy diethylene glycol acrylate) (PMDEGA) block, various gelation behaviors and switching temperatures were achieved. New RAFT agents bearing tert-butyl benzoate or benzoic acid groups, were developed for the synthesis of diblock, symmetrical triblock and 3-arm star block copolymers. Thus, specific end groups were attached to the polymers that facilitate efficient macromolecular characterization, e.g by routine 1H-NMR spectroscopy. Further, the carboxyl end-groups allowed functionalizing the various polymers by a fluorophore. Because reports on PMDEGA have been extremely rare, at first, the thermo-responsive behavior of the polymer was investigated and the influence of factors such as molar mass, nature of the end-groups, and architecture, was studied. The use of special RAFT agents enabled the design of polymer with specific hydrophobic and hydrophilic end-groups. Cloud points (CP) of the polymers proved to be sensitive to all molecular variables studied, namely molar mass, nature and number of the end-groups, up to relatively high molar masses. Thus, by changing molecular parameters, CPs of the PMDEGA could be easily adjusted within the physiological interesting range of 20 to 40°C. A second responsivity, namely to light, was added to the PMDEGA system via random copolymerization of MDEGA with a specifically designed photo-switchable azobenzene acrylate. The composition of the copolymers was varied in order to determine the optimal conditions for an isothermal cloud point variation triggered by light. Though reversible light-induced solubility changes were achieved, the differences between the cloud points before and after the irradiation were small. Remarkably, the response to light differed from common observations for azobenzene-based systems, as CPs decreased after UV-irradiation, i.e with increasing content of cis-azobenzene units. The viscosifying and gelling abilities of the various block copolymers made from PS and PMDEGA blocks were studied by rheology. Important differences were observed between diblock copolymers, containing one hydrophobic PS block only, the telechelic symmetrical triblock copolymers made of two associating PS termini, and the star block copolymers having three associating end blocks. Regardless of their hydrophilic block length, diblock copolymers PS11 PMDEGAn were freely flowing even at concentrations as high as 40 wt. \%. In contrast, all studied symmetrical triblock copolymers PS8-PMDEGAn-PS8 formed gels at low temperatures and at concentrations as low as 3.5 wt. \% at best. When heated, these gels underwent a gel-sol transition at intermediate temperatures, well below the cloud point where phase separation occurs. The gel-sol transition shifted to markedly higher transition temperatures with increasing length of the hydrophilic inner block. This effect increased also with the number of arms, and with the length of the hydrophobic end blocks. The mechanical properties of the gels were significantly altered at the cloud point and liquid-like dispersions were formed. These could be reversibly transformed into hydrogels by cooling. This thesis demonstrates that high molar mass PMDEGA is an easily accessible, presumably also biocompatible and at ambient temperature well water-soluble, non-ionic thermo-responsive polymer. PMDEGA can be easily molecularly engineered via the RAFT method, implementing defined end-groups, and producing different, also complex, architectures, such as amphiphilic triblock and star block copolymers, having an analogous structure to associative telechelics. With appropriate design, such amphiphilic copolymers give way to efficient, "smart" viscosifiers and gelators displaying tunable gelling and mechanical properties.}, language = {en} } @misc{OPUS4-4136, title = {Jahresbericht 2010/2011}, volume = {2010/2011}, editor = {Gunnarsson, Logi and Zimmermann, Andreas}, issn = {2191-5857}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59584}, year = {2012}, abstract = {Inhalt: 1. Allgemeiner {\"U}berblick 2. Organisationsstruktur des MRZ 2.1 Angeh{\"o}rige des MRZ 2.2 Beirat 2.3 F{\"o}rderverein 3. Aktivit{\"a}ten im Berichtszeitraum 3.1 Wissenschaftliche Projekte und Veranstaltungen 3.2 Promotionen 3.3 Vortr{\"a}ge und Lehrveranstaltungen 3.4 Publikationen - Neuerscheinungen 2010/2011 4. Anhang - Satzung des MRZ}, language = {de} } @phdthesis{Mari2012, author = {Mari, Andrea}, title = {Signatures of non-classicality in optomechanical systems}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59814}, school = {Universit{\"a}t Potsdam}, year = {2012}, abstract = {This thesis contains several theoretical studies on optomechanical systems, i.e. physical devices where mechanical degrees of freedom are coupled with optical cavity modes. This optomechanical interaction, mediated by radiation pressure, can be exploited for cooling and controlling mechanical resonators in a quantum regime. The goal of this thesis is to propose several new ideas for preparing meso- scopic mechanical systems (of the order of 10^15 atoms) into highly non-classical states. In particular we have shown new methods for preparing optomechani-cal pure states, squeezed states and entangled states. At the same time, proce-dures for experimentally detecting these quantum effects have been proposed. In particular, a quantitative measure of non classicality has been defined in terms of the negativity of phase space quasi-distributions. An operational al- gorithm for experimentally estimating the non-classicality of quantum states has been proposed and successfully applied in a quantum optics experiment. The research has been performed with relatively advanced mathematical tools related to differential equations with periodic coefficients, classical and quantum Bochner's theorems and semidefinite programming. Nevertheless the physics of the problems and the experimental feasibility of the results have been the main priorities.}, language = {en} } @phdthesis{Huang2012, author = {Huang, Shaochun}, title = {Modelling of environmental change impacts on water resources and hydrological extremes in Germany}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59748}, school = {Universit{\"a}t Potsdam}, year = {2012}, abstract = {Water resources, in terms of quantity and quality, are significantly influenced by environmental changes, especially by climate and land use changes. The main objective of the present study is to project climate change impacts on the seasonal dynamics of water fluxes, spatial changes in water balance components as well as the future flood and low flow conditions in Germany. This study is based on the modeling results of the process-based eco-hydrological model SWIM (Soil and Water Integrated Model) driven by various regional climate scenarios on one hand. On the other hand, it is supported by statistical analysis on long-term trends of observed and simulated time series. In addition, this study evaluates the impacts of potential land use changes on water quality in terms of NO3-N load in selected sub-regions of the Elbe basin. In the context of climate change, the actual evapotransipration is likely to increase in most parts of Germany, while total runoff generation may decrease in south and east regions in the scenario period 2051-2060. Water discharge in all six studied large rivers (Ems, Weser, Saale, Danube, Main and Neckar) would be 8 - 30\% lower in summer and autumn compared to the reference period (1961 - 1990), and the strongest decline is expected for the Saale, Danube and Neckar. The 50-year low flow is likely to occur more frequently in western, southern and central Germany after 2061 as suggested by more than 80\% of the model runs. The current low flow period (from August to September) may be extended until the late autumn at the end of this century. Higher winter flow is expected in all of these rivers, and the increase is most significant for the Ems (about 18\%). No general pattern of changes in flood directions can be concluded according to the results driven by different RCMs, emission scenarios and multi-realizations. An optimal agricultural land use and management are essential for the reduction in nutrient loads and improvement of water quality. In the Weiße Elster and Unstrut sub-basins (Elbe), an increase of 10\% in the winter rape area can result in 12-19\% more NO3-N load in rivers. In contrast, another energy plant, maize, has a moderate effect on the water environment. Mineral fertilizers have a much stronger effect on the NO3-N load than organic fertilizers. Cover crops, which play an important role in the reduction of nitrate losses from fields, should be maintained on cropland. The uncertainty in estimating future high flows and, in particular, extreme floods remain high due to different RCM structures, emission scenarios and multi-realizations. In contrast, the projection of low flows under warmer climate conditions appears to be more pronounced and consistent. The largest source of uncertainty related to NO3-N modelling originates from the input data on the agricultural management.}, language = {en} } @phdthesis{Kroll2012, author = {Kroll, Alexander}, title = {Why public managers use performance information : concepts, theory, and empirical analysis}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59795}, school = {Universit{\"a}t Potsdam}, year = {2012}, abstract = {Diese Dissertation untersucht die Verwendung von Performance-Informationen („Kennzahlen") durch F{\"u}hrungskr{\"a}fte in der {\"o}ffentlichen Verwaltung. Unter „Verwendung" wird dabei die zweckorientierte Nutzung der Daten verstanden, um zu steuern, zu lernen und {\"o}ffentliche Leistungen zu verbessern. Die zentrale Frage der Arbeit lautet: Wie k{\"o}nnen Unterschiede bei der Verwendung von Performance-Informationen erkl{\"a}rt werden? Um diese Frage zu beantworten, wird die bereits existierende Literatur strukturiert ausgewertet. Forschungsl{\"u}cken werden aufgezeigt und eigene Ans{\"a}tze vorgestellt, wie diese geschlossen werden k{\"o}nnen. Der erste Teil der Dissertation untersucht den Einfluss von Manager-bezogenen Faktoren auf die Nutzung von Performance-Daten, die bislang in der Forschung noch keine Ber{\"u}cksichtigung gefunden haben. Der zweite Abschnitt testet ein modifiziertes Modell aus der Psychologie, das auf der Annahme basiert, dass die Verwendung von Performance-Informationen ein bewusstes und durchdachtes Verhalten darstellt. Der dritte Teil untersucht, inwieweit sich die Erkl{\"a}rungen f{\"u}r die Nutzung von Performance-Informationen unterscheiden, wenn wir diese nicht nur als Kennzahlen definieren, sondern ebenfalls andere Quellen von „unsystematischem" Feedback ber{\"u}cksichtigen. Die empirischen Ergebnisse der Arbeit basieren auf einer Umfrage aus dem Jahr 2011. Im Rahmen dieses Surveys habe ich die mittleren Manager (Amtsleiter und Fachbereichsleiter) aus acht ausgew{\"a}hlten Bereichen aller kreisfreien St{\"a}dte in Deutschland befragt (n=954). Zur Auswertung der Daten wurden die Verfahren Faktorenanalyse, Multiple Regressionsanalyse und Strukturgleichungsmodellierung eingesetzt. Meine Forschung f{\"o}rderte unter anderem vier Erkenntnisse zu Tage, die durch {\"a}hnliche Befunde der verschiedenen Teile der Dissertation abgesichert sind: 1) Die Verwendung von Performance-Daten kann als bewusstes Verhalten von F{\"u}hrungskr{\"a}ften modelliert werden, das durch deren Einstellung sowie durch die Einstellung ihres sozialen Umfeldes bestimmt wird. 2) H{\"a}ufige Nutzer von Performance-Informationen zeigen {\"u}berraschenderweise keine generelle Pr{\"a}ferenz f{\"u}r das abstrakt-analytische Verarbeiten von Informationen. Stattdessen bevorzugen sie, Informationen durch pers{\"o}nliche Interaktionen aufzunehmen. 3) Manager, die sich fr{\"u}h im Rahmen der Ermittlung von Performance-Informationen engagieren, nutzen diese sp{\"a}ter auch h{\"a}ufiger, um Entscheidungen zu treffen. 4) Performance-Berichte sind nur eine Informationsquelle unter vielen. Verwaltungsmanager pr{\"a}ferieren verbales Feedback von Insidern sowie das Feedback von wichtigen externen Stakeholdern gegen{\"u}ber systematischen Performance-Daten. Die Dissertation erkl{\"a}rt diese Befunde theoretisch und verdeutlicht deren Implikationen f{\"u}r Theorie und Praxis.}, language = {en} } @book{KrauseGiese2012, author = {Krause, Christian and Giese, Holger}, title = {Quantitative modeling and analysis of service-oriented real-time systems using interval probabilistic timed automata}, publisher = {Universit{\"a}tsverlah Potsdam}, address = {Potsdam}, isbn = {978-3-86956-171-4}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-57845}, publisher = {Universit{\"a}t Potsdam}, pages = {45}, year = {2012}, abstract = {One of the key challenges in service-oriented systems engineering is the prediction and assurance of non-functional properties, such as the reliability and the availability of composite interorganizational services. Such systems are often characterized by a variety of inherent uncertainties, which must be addressed in the modeling and the analysis approach. The different relevant types of uncertainties can be categorized into (1) epistemic uncertainties due to incomplete knowledge and (2) randomization as explicitly used in protocols or as a result of physical processes. In this report, we study a probabilistic timed model which allows us to quantitatively reason about nonfunctional properties for a restricted class of service-oriented real-time systems using formal methods. To properly motivate the choice for the used approach, we devise a requirements catalogue for the modeling and the analysis of probabilistic real-time systems with uncertainties and provide evidence that the uncertainties of type (1) and (2) in the targeted systems have a major impact on the used models and require distinguished analysis approaches. The formal model we use in this report are Interval Probabilistic Timed Automata (IPTA). Based on the outlined requirements, we give evidence that this model provides both enough expressiveness for a realistic and modular specifiation of the targeted class of systems, and suitable formal methods for analyzing properties, such as safety and reliability properties in a quantitative manner. As technical means for the quantitative analysis, we build on probabilistic model checking, specifically on probabilistic time-bounded reachability analysis and computation of expected reachability rewards and costs. To carry out the quantitative analysis using probabilistic model checking, we developed an extension of the Prism tool for modeling and analyzing IPTA. Our extension of Prism introduces a means for modeling probabilistic uncertainty in the form of probability intervals, as required for IPTA. For analyzing IPTA, our Prism extension moreover adds support for probabilistic reachability checking and computation of expected rewards and costs. We discuss the performance of our extended version of Prism and compare the interval-based IPTA approach to models with fixed probabilities.}, language = {en} } @phdthesis{Ott2012, author = {Ott, Susan}, title = {Feld - f{\"a}llt - fehlt : Untersuchungen zur Phonologie-Morphosyntax-Schnittstelle bei Kindern und Erwachsenen}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-161-5}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-57798}, school = {Universit{\"a}t Potsdam}, pages = {379}, year = {2012}, abstract = {Neben der Frequenz eines cues ist es dessen Zuverl{\"a}ssigkeit, die Kindern hilft, die an sie gerichtete Sprache zu segmentieren, Worteinheiten zu erkennen sowie diese syntaktisch zu kategorisieren. Im Deutschen weist die Subsilbe „Langvokal+Konsonant+/t/" (z.B. in fehlt, wohnt) zuverl{\"a}ssig auf eine -t-flektierte Verbform hin. Die in kindgerichteter Sprache h{\"o}her frequente Subsilbe „Kurzvokal+Konsonant+/t/" (z.B. in Feld, Hemd, f{\"a}llt, rund) gibt hingegen keinen derartig eindeutigen Hinweis. Es wurde der Frage nachgegangen, inwiefern diese unterschiedlichen Zuverl{\"a}ssigkeiten und Frequenzen der Subsilben auf die Nomen-, Verb- und Verbflexionsverarbeitung einwirken. Drei Altersgruppen wurden untersucht: achtzehn Monate alte Kinder, drei- bis f{\"u}nfj{\"a}hrige sprachunauff{\"a}llige und -auff{\"a}llige Kinder sowie erwachsene Sprecher. Einfl{\"u}sse der unterschiedlichen Zuverl{\"a}ssigkeiten und Frequenzen der ausgew{\"a}hlten Subsilben konnten f{\"u}r alle Probandengruppen gefunden werden. Die Subsilbe stellt damit eine linguistische Gr{\"o}ße dar, die in der fr{\"u}hen Sprachwahrnehmung als cue dienen sowie die Sprachverarbeitung Erwachsener lenken kann und auch f{\"u}r die Sprachdiagnostik und -therapie sprachauff{\"a}lliger Kinder ber{\"u}cksichtigt werden sollte.}, language = {de} } @phdthesis{Falco2012, author = {Falco, Camillo}, title = {Sustainable biomass-derived hydrothermal carbons for energy applications}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59785}, school = {Universit{\"a}t Potsdam}, year = {2012}, abstract = {The need to reduce humankind reliance on fossil fuels by exploiting sustainably the planet renewable resources is a major driving force determining the focus of modern material research. For this reason great interest is nowadays focused on finding alternatives to fossil fuels derived products/materials. For the short term the most promising substitute is undoubtedly biomass, since it is the only renewable and sustainable alternative to fossil fuels as carbon source. As a consequence efforts, aimed at finding new synthetic approaches to convert biomass and its derivatives into carbon-based materials, are constantly increasing. In this regard, hydrothermal carbonisation (HTC) has shown to be an effective means of conversion of biomass-derived precursors into functional carbon materials. However the attempts to convert raw biomass, in particular lignocellulosic one, directly into such products have certainly been rarer. Unlocking the direct use of these raw materials as carbon precursors would definitely be beneficial in terms of HTC sustainability. For this reason, in this thesis the HTC of carbohydrate and protein-rich biomass was systematically investigated, in order to obtain more insights on the potentials of this thermochemical processing technique in relation to the production of functional carbon materials from crude biomass. First a detailed investigation on the HTC conversion mechanism of lignocellulosic biomass and its single components (i.e. cellulose, lignin) was developed based on a comparison with glucose HTC, which was adopted as a reference model. In the glucose case it was demonstrated that varying the HTC temperature allowed tuning the chemical structure of the synthesised carbon materials from a highly cross-linked furan-based structure (T = 180oC) to a carbon framework composed of polyaromatic arene-like domains. When cellulose or lignocellulosic biomass was used as carbon precursor, the furan rich structure could not be isolated at any of the investigated processing conditions. These evidences were indicative of a different HTC conversion mechanism for cellulose, involving reactions that are commonly observed during pyrolytic processes. The evolution of glucose-derived HTC carbon chemical structure upon pyrolysis was also investigated. These studies revealed that upon heat treatment (Investigated temperatures 350 - 900 oC) the furan-based structure was progressively converted into highly curved aromatic pre-graphenic domains. This thermal degradation process was observed to produce an increasingly more hydrophobic surface and considerable microporosity within the HTC carbon structure. In order to introduce porosity in the HTC carbons derived from lignocellulosic biomass, KOH chemical activation was investigated as an HTC post-synthesis functionalisation step. These studies demonstrated that HTC carbons are excellent precursors for the production of highly microporous activated carbons (ACs) and that the porosity development upon KOH chemical activation is dependent on the chemical structure of the HTC carbon, tuned by employing different HTC temperatures. Preliminary testing of the ACs for CO2 capture or high pressure CH4 storage yielded very promising results, since the measured uptakes of both adsorbates (i.e. CO2 and CH4) were comparable to top-performing and commercially available adsorbents, usually employed for these end-applications. The combined use of HTC and KOH chemical activation was also employed to produce highly microporous N-doped ACs from microalgae. The hydrothermal treatment of the microalgae substrate was observed to cause the depletion of the protein and carbohydrate fractions and the near complete loss (i.e. 90\%) of the microalgae N-content, as liquid hydrolysis/degradation products. The obtained carbonaceous product showed a predominantly aliphatic character indicating the presence of alkyl chains presumably derived from the lipid fractions. Addition of glucose to the initial reaction mixture was found out to be extremely beneficial, because it allowed the fixation of a higher N amount, in the algae derived HTC carbons (i.e.  60\%), and the attainment of higher product yields (50\%). Both positive effects were attributed to Maillard type cascade reactions taking place between the monosaccharides and the microalgae derived liquid hydrolysis/degradation products, which were in this way recovered from the liquid phase. KOH chemical activation of the microalgae/glucose mixture derived HTC carbons produced highly microporous N-doped carbons. Although the activation process led to a major reduction of the N-content, the retained N-amount in the ACs was still considerable. These features render these materials ideal candidates for supercapacitors electrodes, since they provide extremely high surface areas, for the formation of electric double-layer, coupled to abundant heteroatom doping (i.e. N and O) necessary to obtain a pseudocapacitance contribution.}, language = {en} } @book{Haller2012, author = {Haller, Silja Christina}, title = {Wort-Ton-Gestaltung in der Sinfonik Gustav Mahlers}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-159-2}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-55568}, publisher = {Universit{\"a}t Potsdam}, pages = {382}, year = {2012}, abstract = {Die eingehende Analyse ausgew{\"a}hlter Aspekte der Wort-Ton-Gestaltung in Gustav Mahlers Vokalsinfonik ist Schwerpunkt der vorliegenden Untersuchung. Pr{\"a}misse ist Mahlers Ausarbeitung ideenprogrammatischer Intentionen, wobei er sich die besondere St{\"a}rke der Musik, geistige Sachverhalte in gewisser Weise pr{\"a}ziser als Sprache (die hier ins Symbolhafte ausweichen muss) verdeutlichen zu k{\"o}nnen, offensichtlich bewusst zunutze gemacht hatte. Die Differenziertheit der musikalischen Textinterpretation, zugleich Fokus der Analysen, weist deutliche graduelle Unterschiede auf. Sie reicht vom ,Vertonungscharakter' bis hin zur innigsten Anverwandlung von Musik und Textaussage bzw. -semantik. Wesentlich ist das oft proportionale Verh{\"a}ltnis von Grad der Freiheit, die sich Mahler hinsichtlich der Beziehung der Musik zur formalen Topographie des Textes nimmt, zu Intensit{\"a}t und Vielschichtigkeit der musikalischen Textexegese. Je st{\"a}rker sich ihre musikalische Umsetzung in Richtung ‚Semantik-Ausarbeitung' bewegt, verliert die {\"a}ußerliche Sprache an Materialit{\"a}t, wobei das im Lied von der Erde zutage tretende Amalgam von sprachlicher Semantik und Musik gesondert zu nennen ist. Letztendlich wird die {\"u}berwiegend eher unbestimmte Textsemantik durch Mahlers Musik denotiert und in zuvor im Bereich der Vokalsinfonik nicht erreichter Intensit{\"a}t zum Klingen gebracht.}, language = {de} } @phdthesis{CunhaCosta2012, author = {Cunha Costa, Alexandre}, title = {Analyzing and modelling of flow transmission processes in river-systems with a focus on semi-arid conditions}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59694}, school = {Universit{\"a}t Potsdam}, year = {2012}, abstract = {One of the major problems for the implementation of water resources planning and management in arid and semi-arid environments is the scarcity of hydrological data and, consequently, research studies. In this thesis, the hydrology of dryland river systems was analyzed and a semi-distributed hydrological model and a forecasting approach were developed for flow transmission processes in river-systems with a focus on semi-arid conditions. Three different sources of hydrological data (streamflow series, groundwater level series and multi-temporal satellite data) were combined in order to analyze the channel transmission losses of a large reach of the Jaguaribe River in NE Brazil. A perceptual model of this reach was derived suggesting that the application of models, which were developed for sub-humid and temperate regions, may be more suitable for this reach than classical models, which were developed for arid and semi-arid regions. Summarily, it was shown that this river reach is hydraulically connected with groundwater and shifts from being a losing river at the dry and beginning of rainy seasons to become a losing/gaining (mostly losing) river at the middle and end of rainy seasons. A new semi-distributed channel transmission losses model was developed, which was based primarily on the capability of simulation in very different dryland environments and flexible model structures for testing hypotheses on the dominant hydrological processes of rivers. This model was successfully tested in a large reach of the Jaguaribe River in NE Brazil and a small stream in the Walnut Gulch Experimental Watershed in the SW USA. Hypotheses on the dominant processes of the channel transmission losses (different model structures) in the Jaguaribe river were evaluated, showing that both lateral (stream-)aquifer water fluxes and ground-water flow in the underlying alluvium parallel to the river course are necessary to predict streamflow and channel transmission losses, the former process being more relevant than the latter. This procedure not only reduced model structure uncertainties, but also reported modelling failures rejecting model structure hypotheses, namely streamflow without river-aquifer interaction and stream-aquifer flow without groundwater flow parallel to the river course. The application of the model to different dryland environments enabled learning about the model itself from differences in channel reach responses. For example, the parameters related to the unsaturated part of the model, which were active for the small reach in the USA, presented a much greater variation in the sensitivity coefficients than those which drove the saturated part of the model, which were active for the large reach in Brazil. Moreover, a nonparametric approach, which dealt with both deterministic evolution and inherent fluctuations in river discharge data, was developed based on a qualitative dynamical system-based criterion, which involved a learning process about the structure of the time series, instead of a fitting procedure only. This approach, which was based only on the discharge time series itself, was applied to a headwater catchment in Germany, in which runoff are induced by either convective rainfall during the summer or snow melt in the spring. The application showed the following important features: • the differences between runoff measurements were more suitable than the actual runoff measurements when using regression models; • the catchment runoff system shifted from being a possible dynamical system contaminated with noise to a linear random process when the interval time of the discharge time series increased; • and runoff underestimation can be expected for rising limbs and overestimation for falling limbs. This nonparametric approach was compared with a distributed hydrological model designed for real-time flood forecasting, with both presenting similar results on average. Finally, a benchmark for hydrological research using semi-distributed modelling was proposed, based on the aforementioned analysis, modelling and forecasting of flow transmission processes. The aim of this benchmark was not to describe a blue-print for hydrological modelling design, but rather to propose a scientific method to improve hydrological knowledge using semi-distributed hydrological modelling. Following the application of the proposed benchmark to a case study, the actual state of its hydrological knowledge and its predictive uncertainty can be determined, primarily through rejected hypotheses on the dominant hydrological processes and differences in catchment/variables responses.}, language = {en} } @unpublished{Nehring2012, author = {Nehring, Benjamin}, title = {Construction of point processes for classical and quantum gases}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59648}, year = {2012}, abstract = {We propose a new construction of point processes, which generalizes the class of infinitely divisible point processes. Examples are the quantum Boson and Fermion gases as well as the classical Gibbs point processes, where the interaction is given by a stable and regular pair potential.}, language = {en} } @unpublished{RattanaBoeckmann2012, author = {Rattana, Amornrat and B{\"o}ckmann, Christine}, title = {Matrix methods for computing Eigenvalues of Sturm-Liouville problems of order four}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59279}, year = {2012}, abstract = {This paper examines and develops matrix methods to approximate the eigenvalues of a fourth order Sturm-Liouville problem subjected to a kind of fixed boundary conditions, furthermore, it extends the matrix methods for a kind of general boundary conditions. The idea of the methods comes from finite difference and Numerov's method as well as boundary value methods for second order regular Sturm-Liouville problems. Moreover, the determination of the correction term formulas of the matrix methods are investigated in order to obtain better approximations of the problem with fixed boundary conditions since the exact eigenvalues for q = 0 are known in this case. Finally, some numerical examples are illustrated.}, language = {en} } @phdthesis{Andres2012, author = {Andres, Dorothee}, title = {Biophysical chemistry of lipopolysaccharide specific bacteriophages}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59261}, school = {Universit{\"a}t Potsdam}, year = {2012}, abstract = {Carbohydrate recognition is a ubiquitous principle underlying many fundamental biological processes like fertilization, embryogenesis and viral infections. But how carbohydrate specificity and affinity induce a molecular event is not well understood. One of these examples is bacteriophage P22 that binds and infects three distinct Salmonella enterica (S.) hosts. It recognizes and depolymerizes repetitive carbohydrate structures of O antigen in its host´s outer membrane lipopolysaccharide molecule. This is mediated by tailspikes, mainly β helical appendages on phage P22 short non contractile tail apparatus (podovirus). The O antigen of all three Salmonella enterica hosts is built from tetrasaccharide repeating units consisting of an identical main chain with a distinguished 3,6 dideoxyhexose substituent that is crucial for P22 tailspike recognition: tyvelose in S. Enteritidis, abequose in S. Typhimurium and paratose in S. Paratyphi. In the first study the complexes of P22 tailspike with its host's O antigen octasaccharide were characterized. S. Paratyphi octasaccharide binds less tightly (ΔΔG≈7 kJ/mol) to the tailspike than the other two hosts. Crystal structure analysis of P22 tailspike co crystallized with S. Paratyphi octasaccharides revealed different interactions than those observed before in tailspike complexes with S. Enteritidis and S. Typhimurium octasaccharides. These different interactions occur due to a structural rearrangement in the S. Paratyphi octasaccharide. It results in an unfavorable glycosidic bond Φ/Ψ angle combination that also had occurred when the S. Paratyphi octasaccharide conformation was analyzed in an aprotic environment. Contributions of individual protein surface contacts to binding affinity were analyzed showing that conserved structural waters mediate specific recognition of all three different Salmonella host O antigens. Although different O antigen structures possess distinct binding behavior on the tailspike surface, all are recognized and infected by phage P22. Hence, in a second study, binding measurements revealed that multivalent O antigen was able to bind with high avidity to P22 tailspike. Dissociation rates of the polymer were three times slower than for an octasaccharide fragment pointing towards high affinity for O antigen polysaccharide. Furthermore, when phage P22 was incubated with lipopolysaccharide aggregates before plating on S. Typhimurium cells, P22 infectivity became significantly reduced. Therefore, in a third study, the function of carbohydrate recognition on the infection process was characterized. It was shown that large S. Typhimurium lipopolysaccharide aggregates triggered DNA release from the phage capsid in vitro. This provides evidence that phage P22 does not use a second receptor on the Salmonella surface for infection. P22 tailspike binding and cleavage activity modulate DNA egress from the phage capsid. DNA release occurred more slowly when the phage possessed mutant tailspikes with less hydrolytic activity and was not induced if lipopolysaccharides contained tailspike shortened O antigen polymer. Furthermore, the onset of DNA release was delayed by tailspikes with reduced binding affinity. The results suggest a model for P22 infection induced by carbohydrate recognition: tailspikes position the phage on Salmonella enterica and their hydrolytic activity forces a central structural protein of the phage assembly, the plug protein, onto the host´s membrane surface. Upon membrane contact, a conformational change has to occur in the assembly to eject DNA and pilot proteins from the phage to establish infection. Earlier studies had investigated DNA ejection in vitro solely for viruses with long non contractile tails (siphovirus) recognizing protein receptors. Podovirus P22 in this work was therefore the first example for a short tailed phage with an LPS recognition organelle that can trigger DNA ejection in vitro. However, O antigen binding and cleaving tailspikes are widely distributed in the phage biosphere, for example in siphovirus 9NA. Crystal structure analysis of 9NA tailspike revealed a complete similar fold to P22 tailspike although they only share 36 \% sequence identity. Moreover, 9NA tailspike possesses similar enzyme activity towards S. Typhimurium O antigen within conserved amino acids. These are responsible for a DNA ejection process from siphovirus 9NA triggered by lipopolysaccharide aggregates. 9NA expelled its DNA 30 times faster than podovirus P22 although the associated conformational change is controlled with a similar high activation barrier. The difference in DNA ejection velocity mirrors different tail morphologies and their efficiency to translate a carbohydrate recognition signal into action.}, language = {en} } @book{OPUS4-5731, title = {Proceedings of the 4th Many-core Applications Research Community (MARC) Symposium}, editor = {Tr{\"o}ger, Peter and Polze, Andreas}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-169-1}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-57898}, publisher = {Universit{\"a}t Potsdam}, pages = {82}, year = {2012}, abstract = {In continuation of a successful series of events, the 4th Many-core Applications Research Community (MARC) symposium took place at the HPI in Potsdam on December 8th and 9th 2011. Over 60 researchers from different fields presented their work on many-core hardware architectures, their programming models, and the resulting research questions for the upcoming generation of heterogeneous parallel systems.}, language = {en} } @book{Hickmann2012, author = {Hickmann, Thomas}, title = {Das Verh{\"a}ltnis von Wissenschaft und Politik in internationalen Umweltregimen}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-163-9}, issn = {1868-6222}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-57003}, publisher = {Universit{\"a}tsverlag Potsdam}, pages = {110}, year = {2012}, abstract = {Wissenschaft hat einen großen Anteil daran, internationale Umweltprobleme auf die politische Tagesordnung zu bringen und Maßnahmen zu ihrer technischen L{\"o}sung zu entwerfen. Dies gilt sowohl f{\"u}r den Abbau der Ozonschicht als auch f{\"u}r den Klimawandel. So relevant die Generierung von wissenschaftlichen Erkenntnissen in diesen Feldern ist, so komplex ist die Interaktion zwischen Wissenschaft und Politik und so schwierig ist es, Verl{\"a}ssliches dar{\"u}ber zu sagen. Thomas Hickmann analysiert das Verh{\"a}ltnis von Wissenschaft und Politik in internationalen Umweltregimen aus einer neuen Perspektive. Er untersucht nicht den Einfluss wissenschaftlicher Erkenntnisse auf die Politik, sondern r{\"u}ckt die Frage in den Fokus, inwieweit Regierungen Einfluss auf die Wissenschaft nehmen, um nationale Interessen durchzusetzen. Diese Perspektive wurde in der Forschung bislang weitgehend vernachl{\"a}ssigt, weshalb eine systematische Analyse dieser Frage in der Literatur fehlt.}, language = {de} } @book{Klein2012, author = {Klein, Tanja}, title = {Rechtspopulistische Parteien in Regierungsbildungsprozessen}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-164-6}, issn = {1866-0738}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-57703}, publisher = {Universit{\"a}t Potsdam}, pages = {133}, year = {2012}, abstract = {Diese Publikation einer Masterarbeit leistet einen Beitrag, die bestehende Forschungsl{\"u}cke hinsichtlich der Rolle rechtspopulistischer Parteien in Regierungsbildungsprozessen zu f{\"u}llen. Warum haben sich etablierte Parteien und Rechtspopulisten f{\"u}r eine bestimmte Konstellation entschieden? Welche Probleme traten durch den spezifisch rechtspopulistischen Charakter bei der Regierungsbildung auf? Welche Alternativen gibt es? Mittels eines vergleichenden Ansatzes werden dabei als Fallbeispiele die Regierungsbildungsprozesse nach drei Wahlen zu nationalen Parlamenten im Jahr 2010 in Belgien, den Niederlanden und Schweden analysiert.}, language = {de} }