@phdthesis{Federico2011,
  author    = {Federico, Stefania},
  title     = {Synthetic peptides derived from decorin as building blocks for biomaterials based on supramolecular interactions},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59661},
  school      = {Universit{\"a}t Potsdam},
  year      = {2011},
  abstract  = {In this work, the development of a new molecular building block, based on synthetic peptides derived from decorin, is presented. These peptides represent a promising basis for the design of polymer-based biomaterials that mimic the ECM on a molecular level and exploit specific biological recognition for technical applications. Multiple sequence alignments of the internal repeats of decorin that formed the inner and outer surface of the arch-shaped protein were used to develop consensus sequences. These sequences contained conserved sequence motifs that are likely to be related to structural and functional features of the protein. Peptides representative for the consensus sequences were synthesized by microwave-assisted solid phase peptide synthesis and purified by RP-HPLC, with purities higher than 95 mol\%. After confirming the desired masses by MALDI-TOF-MS, the primary structure of each peptide was investigated by 1H and 2D NMR, from which a full assignment of the chemical shifts was obtained. The characterization of the peptides conformation in solution was performed by CD spectroscopy, which demonstrated that using TFE, the peptides from the outer surface of decorin show a high propensity to fold into helical structures as observed in the original protein. To the contrary, the peptides from the inner surface did not show propensity to form stable secondary structure. The investigation of the binding capability of the peptides to Collagen I was performed by surface plasmon resonance analyses, from which all but one of the peptides representing the inner surface of decorin showed binding affinity to collagen with values of dissociation constant between 2•10-7 M and 2.3•10-4 M. On the other hand, the peptides representative for the outer surface of decorin did not show any significant interaction to collagen. This information was then used to develop experimental demonstration for the binding capabilities of the peptides from the inner surface of decorin to collagen even when used in more complicated situations close to possible appications. With this purpose, the peptide (LRELHLNNN) which showed the highest binding affinity to collagen (2•10-7 M) was functionalized with an N-terminal triple bond in order to obtain a peptide dimer via copper(I)-catalyzed cycloaddition reaction with 4,4'-diazidostilbene-2,2'-disulfonic acid. Rheological measurements showed that the presence of the peptide dimer was able to enhance the elastic modulus (G') of a collagen gel from ~ 600 Pa (collagen alone) to ~ 2700 Pa (collagen and peptide dimer). Moreover, it was shown that the mechanical properties of a collagen gel can be tailored by using different molar ratios of peptide dimer respect to collagen. The same peptide, functionalized with the triple bond, was used to obtain a peptide-dye conjugate by coupling it with N-(5'-azidopentanoyl)-5-aminofluorescein. An aqueous solution (5 vol\% methanol) of the peptide dye conjugate was injected into a collagen and a hyaluronic acid (HA) gel and images of fluorescence detection showed that the diffusion of the peptide was slower in the collagen gel compared to the HA gel. The third experimental demonstration was gained using the peptide (LSELRLHNN) which showed the lower binding affinity (2.3•10-4 M) to collagen. This peptide was grafted to hyaluronic acid via EDC-chemistry, with a degree of functionalization of 7 ± 2 mol\% as calculated by 1H-NMR. The grafting was further confirmed by FTIR and TGA measurements, which showed that the onset of decomposition for the HA-g-peptide decreased by 10 °C compared to the native HA. Rheological measurements showed that the elastic modulus of a system based on collagen and HA-g-peptide increased by almost two order of magnitude (G' = 200 Pa) compared to a system based on collagen and HA (G' = 0.9 Pa). Overall, this study showed that the synthetic peptides, which were identified from decorin, can be applied as potential building blocks for biomimetic materials that function via biological recognition.},
  language  = {en}
}
@phdthesis{Gessner2011,
  author    = {Geßner, Andr{\´e}},
  title     = {Neuartige Lanthanoid-dotierte mikro- und mesopor{\"o}se Feststoffe : Charakterisierung von Ion-Wirt-Wechselwirkungen, Speziesverteilung und Lumineszenzeigenschaften mittels zeitaufgel{\"o}ster Lumineszenzspektroskopie},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-52371},
  school      = {Universit{\"a}t Potsdam},
  year      = {2011},
  abstract  = {Por{\"o}se Sol-Gel-Materialien finden in vielen Bereichen Anwendung bzw. sind Gegenstand der aktuellen Forschung. Zu diesen Bereichen z{\"a}hlen sowohl klassische Anwendungen, wie z. B. die Verwendung als Katalysator, Molekularsieb oder Trockenmittel, als auch nichtklassische Anwendungen, wie z. B. der Einsatz als Kontrastmittel in der Magnet-Resonanz-Tomographie oder in Form von d{\"u}nnen Zeolithfilmen als Isolatoren in Mikrochips. Auch f{\"u}r den Einsatz in der Photonik werden por{\"o}se Materialien in Betracht gezogen, wie die Entwicklung des Zeolith-Farbstoff-Lasers zeigt. Mikropor{\"o}se Zeolithe k{\"o}nnen generell {\"u}ber einfache Ionenaustauschreaktionen mit Lanthanoidionen in lumineszente Materialien umgewandelt werden. Neben der Erzeugung eines lumineszenten Materials, dessen Lumineszenzeigenschaften charakterisiert werden m{\"u}ssen, bietet die Nutzung von Lanthanoidionen die M{\"o}glichkeit diese Ionen als Sonde zur Charakterisierung der Ion-Wirt-Wechselwirkungen zu funktionalisieren, was z. B. in Bezug auf die Anwendung als Katalysator von großer Bedeutung ist. Dabei werden die einzigartigen Lumineszenzeigenschaften der Lanthanoidionen, in diesem Fall von Europium(III) und Terbium(III), genutzt. In dieser Arbeit wurden Lanthanoid-dotierte mikropor{\"o}se Zeolithe, mikropor{\"o}s-mesopor{\"o}se Hybridmaterialien und mesopor{\"o}se Silikate hinsichtlich ihrer Lumineszenzeigenschaften und ihrer Wechselwirkung des Wirtsmaterials mit den Lanthanoidionen mittels zeitaufgel{\"o}ster Lumineszenzspektroskopie untersucht. Zeitaufgel{\"o}ste Emissionsspektren (TRES) liefern dabei sowohl Informationen in der Wellenl{\"a}ngen- als auch in der Zeitdom{\"a}ne. Erstmalig wurden die TRES mittels einer umfangreichen Auswertemethodik behandelt. Neben der Anpassung des Abklingverhaltens mit einer diskreten Zahl von Exponentialfunktionen, wurden unterst{\"u}tzend auch Abklingzeitverteilungsanalysen durchgef{\"u}hrt. Zeitaufgel{\"o}ste fl{\"a}chennormierte Emissionsspektren (TRANES), eine Erweiterung der normalen TRES, konnten erstmals zur Bestimmung der Zahl der emittierenden Lanthanoidspezies in por{\"o}sen Materialien genutzt werden. Durch die Berechnung der Decayassoziierten Spektren (DAS) konnten den Lanthanoidspezies die entsprechenden Lumineszenzspektren zugeordnet werden. Zus{\"a}tzlich konnte, speziell im Fall der Europium-Lumineszenz, durch Kombination von zeitlicher und spektraler Information das zeitabh{\"a}ngige Asymmetrieverh{\"a}ltnis R und die spektrale Evolution des 5D0-7F0-{\"U}bergangs mit der Zeit t untersucht und somit wesentliche Informationen {\"u}ber die Verteilung der Europiumionen im Wirtsmaterial erhalten werden. {\"U}ber die Abklingzeit und das Asymmetrieverh{\"a}ltnis R konnten R{\"u}ckschl{\"u}sse auf die Zahl der OH-Oszillatoren in der ersten Koordinationssph{\"a}re und die Symmetrie der Koordinationsumgebung gezogen werden. F{\"u}r die mikropor{\"o}sen und mikropor{\"o}s-mesopor{\"o}sen Materialien wurden verschiedene Lanthanoidspezies, im Regelfall zwei, gefunden, welche entsprechend der beschriebenen Methoden charakterisiert wurden. Diese Lanthanoidspezies konnten Positionen in den Materialien zugeordnet werden, die sich im tief Inneren des Porensystems oder auf bzw. nahe der {\"a}ußeren Oberfl{\"a}che oder in den Mesoporen befinden. Erstere Spezies ist aufgrund ihrer Position im Material gut vor Feuchtigkeitseinfl{\"u}ssen gesch{\"u}tzt, was sich deutlich in entsprechend langen Lumineszenzabklingzeiten {\"a}ußert. Zus{\"a}tzlich ist diese Europiumspezies durch unsymmetrische Koordinationsumgebung charakterisiert, was auf einen signifikanten Anteil an Koordination der Lanthanoidionen durch die Sauerstoffatome im Wirtsgitter zur{\"u}ckzuf{\"u}hren ist. Ionen, die sich nahe oder auf der {\"a}ußeren Oberfl{\"a}che befinden, sind dagegen f{\"u}r Feuchtigkeit zug{\"a}nglicher, was in k{\"u}rzeren Lumineszenzabklingzeiten und einer symmetrischeren Koordinationsumgebung resultiert. Der Anteil von Wassermolek{\"u}len in der ersten Koordinationssph{\"a}re ist hier deutlich gr{\"o}ßer, als bei den Ionen, die sich tiefer im Porensystem befinden und entspricht in vielen F{\"a}llen der Koordinationszahl eines vollst{\"a}ndig hydratisierten Lanthanoidions. Auch der Einfluss von Oberfl{\"a}chenmodifikationen auf die Speziesverteilung und das Verhalten der Materialien gegen{\"u}ber Feuchtigkeit wurde untersucht. Dabei gelang es den Einfluss der Feuchtigkeit auf die Lumineszenzeigenschaften und die Speziesverteilung durch die Oberfl{\"a}chenmodifikation zu verringern und die Lumineszenzeigenschaften teilweise zu konservieren. Im Fall der mesopor{\"o}sen Silikamonolithe wurde auch eine heterogene Verteilung der Lanthanoidionen im Porensystem gefunden. Hier wechselwirkt ein Teil der Ionen mit der Porenwand, w{\"a}hrend sich die restlichen Ionen in der w{\"a}ßrigen Phase innerhalb des Porensystems aufhalten. Das Aufbringen von Oberfl{\"a}chenmodifikationen f{\"u}hrte zu einer Wechselwirkung der Ionen mit diesen Oberfl{\"a}chenmodifikationen, was sich in Abh{\"a}ngigkeit von der Oberfl{\"a}chenbeladung in den enstprechenden Lumineszenzeigenschaften niederschlug.},
  language  = {de}
}
@phdthesis{Weiss2011,
  author    = {Weiß, Jan},
  title     = {Synthesis and self-assembly of multiple thermoresponsive amphiphilic block copolymers},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-53360},
  school      = {Universit{\"a}t Potsdam},
  year      = {2011},
  abstract  = {In the present thesis, the self-assembly of multi thermoresponsive block copolymers in dilute aqueous solution was investigated by a combination of turbidimetry, dynamic light scattering, TEM measurements, NMR as well as fluorescence spectroscopy. The successive conversion of such block copolymers from a hydrophilic into a hydrophobic state includes intermediate amphiphilic states with a variable hydrophilic-to-lipophilic balance. As a result, the self-organization is not following an all-or-none principle but a multistep aggregation in dilute solution was observed. The synthesis of double thermoresponsive diblock copolymers as well as triple thermoresponsive triblock copolymers was realized using twofold-TMS labeled RAFT agents which provide direct information about the average molar mass as well as residual end group functionality from a routine proton NMR spectrum. First a set of double thermosensitive diblock copolymers poly(N-n-propylacrylamide)-b-poly(N-ethylacrylamide) was synthesized which differed only in the relative size of the two blocks. Depending on the relative block lengths, different aggregation pathways were found. Furthermore, the complementary TMS-labeled end groups served as NMR-probes for the self-assembly of these diblock copolymers in dilute solution. Reversible, temperature sensitive peak splitting of the TMS-signals in NMR spectroscopy was indicative for the formation of mixed star-/flower-like micelles in some cases. Moreover, triple thermoresponsive triblock copolymers from poly(N-n-propylacrylamide) (A), poly(methoxydiethylene glycol acrylate) (B) and poly(N-ethylacrylamide) (C) were obtained from sequential RAFT polymerization in all possible block sequences (ABC, BAC, ACB). Their self-organization behavior in dilute aqueous solution was found to be rather complex and dependent on the positioning of the different blocks within the terpolymers. Especially the localization of the low-LCST block (A) had a large influence on the aggregation behavior. Above the first cloud point, aggregates were only observed when the A block was located at one terminus. Once placed in the middle, unimolecular micelles were observed which showed aggregation only above the second phase transition temperature of the B block. Carrier abilities of such triple thermosensitive triblock copolymers tested in fluorescence spectroscopy, using the solvatochromic dye Nile Red, suggested that the hydrophobic probe is less efficiently incorporated by the polymer with the BAC sequence as compared to ABC or ACB polymers above the first phase transition temperature. In addition, due to the problem of increasing loss of end group functionality during the subsequent polymerization steps, a novel concept for the one-step synthesis of multi thermoresponsive block copolymers was developed. This allowed to synthesize double thermoresponsive di- and triblock copolymers in a single polymerization step. The copolymerization of different N-substituted maleimides with a thermosensitive styrene derivative (4-vinylbenzyl methoxytetrakis(oxyethylene) ether) led to alternating copolymers with variable LCST. Consequently, an excess of this styrene-based monomer allowed the synthesis of double thermoresponsive tapered block copolymers in a single polymerization step.},
  language  = {en}
}
@article{HassReich2011,
  author    = {Hass, Roland and Reich, Oliver},
  title     = {Photon density wave spectroscopy for dilution-free sizing of highly concentrated nanoparticles during starved-feed polymerization},
  series = {ChemPhysChem : a European journal of chemical physics and physical chemistry},
  volume    = {12},
  journal   = {ChemPhysChem : a European journal of chemical physics and physical chemistry},
  number    = {14},
  publisher = {Wiley-Blackwell},
  address   = {Malden},
  issn      = {1439-4235},
  doi       = {10.1002/cphc.201100323},
  pages     = {2572 -- 2575},
  year      = {2011},
  language  = {en}
}
@article{BresselPrevostAppavouetal.2011,
  author    = {Bressel, Katharina and Prevost, Sylvain and Appavou, Marie-Sousai and Tiersch, Brigitte and Koetz, Joachim and Gradzielski, Michael},
  title     = {Phase behaviour and structure of zwitanionic mixtures of perfluorocarboxylates and tetradecyldimethylamine oxide-dependence on chain length of the perfluoro surfactant},
  series = {Soft matter},
  volume    = {7},
  journal   = {Soft matter},
  number    = {23},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  issn      = {1744-683X},
  doi       = {10.1039/c1sm05618b},
  pages     = {11232 -- 11242},
  year      = {2011},
  abstract  = {Phase behaviour and the mesoscopic structure of zwitanionic surfactant mixtures based on the zwitterionic tetradecyldimethylamine oxide (TDMAO) and anionic lithium perfluoroalkyl carboxylates have been investigated for various chain lengths of the perfluoro surfactant with an emphasis on spontaneously forming vesicles. These mixtures were studied at a constant total concentration of 50 mM and characterised by means of dynamic light scattering (DLS), electric conductivity, small-angle neutron scattering (SANS), viscosity, and cryo-scanning electron microscopy (Cryo-SEM). No vesicles are formed for relatively short perfluoro surfactants. The extension of the vesicle phase becomes substantially larger with increasing chain length of the perfluoro surfactant, while at the same time the size of these vesicles increases. Head group interactions in these systems play a central role in the ability to form vesicles, as already protonating 10 mol\% of the TDMAO largely enhances the propensity for vesicle formation. The range of vesicle formation in the phase diagram is not only substantially enlarged but also extends to shorter perfluoro surfactants, where without protonation no vesicles would be formed. The size and polydispersity of the vesicles are related to the chain length of the perfluoro surfactant, the vesicles becoming smaller and more monodisperse with increasing perfluoro surfactant chain length. The ability of the mixed systems to form well-defined unilamellar vesicles accordingly can be controlled by the length of the alkyl chain of the perfluorinated surfactant and depends strongly on the charge conditions, which can be tuned easily by pH-variation.},
  language  = {en}
}
@article{WellertTierschKoetzetal.2011,
  author    = {Wellert, Stefan and Tiersch, Brigitte and Koetz, Joachim and Richardt, Andre and Lapp, Alain and Holderer, Olaf and Gaeb, Juergen and Blum, Marc-Michael and Schulreich, Christoph and Stehle, Ralf and Hellweg, Thomas},
  title     = {The DFPase from Loligo vulgaris in sugar surfactant-based bicontinuous microemulsions structure, dynamics, and enzyme activity},
  series = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  volume    = {40},
  journal   = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  number    = {6},
  publisher = {Springer},
  address   = {New York},
  issn      = {0175-7571},
  doi       = {10.1007/s00249-011-0689-0},
  pages     = {761 -- 774},
  year      = {2011},
  abstract  = {The enzyme diisopropyl fluorophosphatase (DFPase) from the squid Loligo vulgaris is of great interest because of its ability to catalyze the hydrolysis of highly toxic organophosphates. In this work, the enzyme structure in solution (native state) was studied by use of different scattering methods. The results are compared with those from hydrodynamic model calculations based on the DFPase crystal structure. Bicontinuous microemulsions made of sugar surfactants are discussed as host systems for the DFPase. The microemulsion remains stable in the presence of the enzyme, which is shown by means of scattering experiments. Moreover, activity assays reveal that the DFPase still has high activity in this complex reaction medium. To complement the scattering experiments cryo-SEM was also employed to study the microemulsion structure.},
  language  = {en}
}
@article{WeberTierschUnterlassetal.2011,
  author    = {Weber, Nancy and Tiersch, Brigitte and Unterlass, Miriam M. and Heilig, Anneliese and Tauer, Klaus},
  title     = {"Schizomorphic" Emulsion Copolymerization Particles},
  series = {Macromolecular rapid communications},
  volume    = {32},
  journal   = {Macromolecular rapid communications},
  number    = {23},
  publisher = {Wiley-Blackwell},
  address   = {Malden},
  issn      = {1022-1336},
  doi       = {10.1002/marc.201100491},
  pages     = {1925 -- 1929},
  year      = {2011},
  abstract  = {Cryo-electron microscopy, atomic force microscopy, and light microscopy investigations provide experimental evidence that amphiphilic emulsion copolymerization particles change their morphology in dependence on concentration. The shape of the particles is spherical at solids content above 1\%, but it changes to rod-like, ring-like, and web-like structures at lower concentrations. In addition, the shape and morphology of these particles at low concentrations are not fixed but very flexible and vary with time between spheres, flexible pearlnecklace structures, and stretched rods.},
  language  = {en}
}
@article{KoethAppelhansRobertsonetal.2011,
  author    = {Koeth, Anja and Appelhans, Dietmar and Robertson, Daniela and Tiersch, Brigitte and Koetz, Joachim},
  title     = {Use of weakly cationic dendritic glycopolymer for morphological transformation of phospholipid vesicles into tube-like networks},
  series = {Soft matter},
  volume    = {7},
  journal   = {Soft matter},
  number    = {22},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  issn      = {1744-683X},
  doi       = {10.1039/c1sm06439h},
  pages     = {10581 -- 10584},
  year      = {2011},
  abstract  = {Using cationic polyelectrolytes with different molecular architectures, only hyperbranched poly(ethyleneimine) with maltose shell is suited to tailor the morphological transformation of anionic vesicles into tube-like networks. The interaction features of those materials partly mimic biological features of tubular proteins in nature.},
  language  = {en}
}
@phdthesis{Unterlass2011,
  author    = {Unterlass, Miriam Margarethe},
  title     = {From monomer salts and their tectonic crystals to aromatic polyimides : development of neoteric synthesis routes},
  address   = {Potsdam},
  pages     = {147 S.},
  year      = {2011},
  language  = {en}
}
@phdthesis{Schwadtke2011,
  author    = {Schwadtke, Ulrike},
  title     = {Synthese und Charakterisierung von partiell fluorierten Blockcopolymeren},
  address   = {Potsdam},
  pages     = {117 S.},
  year      = {2011},
  language  = {de}
}
@phdthesis{Frueh2011,
  author    = {Fr{\"u}h, Johannes},
  title     = {Structural change of polyelectrolyte multilayers under mechanical stress},
  address   = {Potsdam},
  pages     = {194 S.},
  year      = {2011},
  language  = {en}
}
@phdthesis{Berger2011,
  author    = {Berger, Ren{\´e}},
  title     = {Die Deacetylierungs- Diazotierungs- Kupplungssequenz : Synthese von Aryldiazoniumtetrafluoroboraten aus Acetaniliden und deren in situ-Umsetzung mit Alken, Alkinen und Kaliumorganifluoroboraten},
  address   = {Potsdam},
  pages     = {215 S.},
  year      = {2011},
  language  = {de}
}
@misc{Fuechsel2011,
  author    = {F{\"u}chsel, Gernot},
  title     = {Elektronengetriebene Reaktionen auf Oberfl{\"a}chen : die Dynamik der femtosekundenlaserinduzierten Desorption von H2/D2 von Ru(001)},
  address   = {Potsdam},
  pages     = {162 S.},
  year      = {2011},
  language  = {de}
}
@phdthesis{Jelicic2011,
  author    = {Jelicic, Aleksandra},
  title     = {Combining polymethacrylates and ionic liquids : a way to create and understand},
  address   = {Potsdam},
  pages     = {161 S.},
  year      = {2011},
  language  = {en}
}
@phdthesis{Berg2011,
  author    = {Berg, John K.},
  title     = {Size-dependent wetting behavior of organic molecules on solid surfaces},
  address   = {Potsdam},
  pages     = {99 S.},
  year      = {2011},
  language  = {en}
}
@phdthesis{Dittrich2011,
  author    = {Dittrich, Matthias},
  title     = {Physical-chemical characterisation of new lipids designed for non-viral gene transfection},
  address   = {Potsdam},
  pages     = {111 S.},
  year      = {2011},
  language  = {en}
}
@phdthesis{Weber2011,
  author    = {Weber, Nancy},
  title     = {Die Synthese "schizomorpher" Copolymer - Latexteilchen},
  address   = {Potsdam},
  pages     = {119 S.},
  year      = {2011},
  language  = {de}
}
@phdthesis{Bramborg2011,
  author    = {Bramborg, Andrea},
  title     = {Regioselektive Synthese von Alkylarenen durch ipso-substitution aromatischer Carbons{\"a}uren},
  address   = {Potsdam},
  pages     = {163 S.},
  year      = {2011},
  language  = {de}
}
@phdthesis{Schenk2011,
  author    = {Schenk, Anna Sophia},
  title     = {On the structure of bio-inspired calcite-polymer hybrid crystal : hierarchical levels from the micrometer- to the †ngstr{\"o}m-scale},
  address   = {Potsdam},
  pages     = {138, XXXIV S.},
  year      = {2011},
  language  = {en}
}
@phdthesis{Geissler2011,
  author    = {Geißler, Diana},
  title     = {Synthese funktionalisierter Furane durch Acrylatmethese und {\"U}bergangsmetall-katalysierte C-C- Verkn{\"u}pfungsreaktionen},
  address   = {Potsdam},
  pages     = {159 S.},
  year      = {2011},
  language  = {de}
}