@misc{NojimaKonishiJaptoketal.2016, author = {Nojima, Hiroyuki and Konishi, Takanori and Japtok, Lukasz and Kleuser, Burkhard and Edwards, Michael J. and Gulbins, Erich and Lentsch, Alex B.}, title = {Chemokine receptors, CXCR1 and CXCR2, differentially regulate exosome release in hepatocytes}, series = {Hepatology : official journal of the American Association for the Study of Liver Diseases}, volume = {64}, journal = {Hepatology : official journal of the American Association for the Study of Liver Diseases}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {0270-9139}, pages = {165A -- 165A}, year = {2016}, language = {en} } @misc{MaierHolzloehnerHoenowetal.2016, author = {Maier, Natalia and Holzl{\"o}hner, Pamela and Hoenow, Anja and Scheunemann, Astrid and Weschke, Daniel and Hanack, Katja}, title = {Characterization of monoclonal antibodies generated by in vitro immunization}, series = {The journal of immunology}, volume = {196}, journal = {The journal of immunology}, publisher = {American Assoc. of Immunologists}, address = {Bethesda}, issn = {0022-1767}, pages = {2}, year = {2016}, abstract = {Monoclonal antibodies are highly valuable tools in biomedicine but the generation by hybridoma technology is very time-consuming and elaborate. In order to circumvent the consisting drawbacks an in vitro immunization approach was established by which murine as well as human monoclonal antibodies against a viral coat protein could be developed. The in vitro immunization process was performed by isolation of murine hematopoietic stem cells or human monocytes and an in vitro differentiation into immature dendritic cells. After antigen loading the cells were co-cultivated with naive T and B lymphocytes for three days in order to obtain antigen-specific B lymphocytes in culture, followed by fusion with murine myeloma cells or human/murine heteromyeloma cells. Antigen-specific hybridomas were selected and the generated antibodies were purified and characterized in this study by ELISA, western blot, gene sequencing, affinity measurements. Further the characteristics were compared to a monoclonal antibody against the same target generated by conventional hybridoma technology. Isotype detection revealed a murine IgM and a human IgG4 antibody in comparison to an IgG1 for the conventionally generated antibody. The antibodies derived from in vitro immunization showed indeed a lower affinity for the antigen as compared to the conventionally generated one, which is probably based on the significantly shorter B cell maturation (3 days) during the immunization process. Nevertheless, they were suitable for building up a sandwich based detection system. Therefore, the in vitro immunization approach seems to be a good and particularly fast alternative to conventional hybridoma technology.}, language = {en} } @misc{HanackSchloerHolzloehneretal.2016, author = {Hanack, Katja and Schloer, Anja and Holzloehner, Pamela and Listek, Martin and Bauer, Cindy and Butze, Monique and Micheel, Burkhard and Hentschel, Christian and Sowa, Mandy and Roggenbuck, Dirk and Schierack, Peter and Fuener, Jonas and Schliebs, Erik and Goihl, Alexander and Reinhold, Dirk}, title = {Camelid nanobodies specific to human pancreatic glycoprotein 2}, series = {The journal of immunology}, volume = {196}, journal = {The journal of immunology}, publisher = {American Assoc. of Immunologists}, address = {Bethesda}, issn = {0022-1767}, pages = {313 -- 328}, year = {2016}, abstract = {Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified to be a major autoantigenic target in Crohn's disease patients. It was discussed recently that a long and a short isoform of GP2 exists whereas the short isoform is often detected by GP2-specific autoantibodies. In the outcome of inflammatory bowel diseases, these GP2-specific autoantibodies are discussed as new serological markers for diagnosis and therapeutic monitoring. To investigate this further, camelid nanobodies were generated by phage display and selected against the short isoform of GP2 in order to isolate specific tools for the discrimination of both isoforms. Nanobodies are single domain antibodies derived from camelid heavy chain only antibodies and characterized by a high stability and solubility. The selected candidates were expressed, purified and validated regarding their binding properties in different enzyme-linked immunosorbent assays formats, immunofluorescence, immunohistochemistry and surface plasmon resonance spectroscopy. Four different nanobodies could be selected whereof three recognize the short isoform of GP2 very specifically and one nanobody showed a high binding capacity for both isoforms. The KD values measured for all nanobodies were between 1.3 nM and 2.3 pM indicating highly specific binders suitable for the application as diagnostic tool in inflammatory bowel disease.}, language = {en} } @misc{MondalHoldt2016, author = {Mondal, Suvendu Sekhar and Holdt, Hans-J{\"u}rgen}, title = {Breaking Down Chemical Weapons by Metal-Organic Frameworks}, series = {Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition}, volume = {55}, journal = {Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1433-7851}, doi = {10.1002/anie.201508407}, pages = {42 -- 44}, year = {2016}, abstract = {Seek and destroy: Filtration schemes and self-detoxifying protective fabrics based on the ZrIV-containing metal—organic frameworks (MOFs) MOF-808 and UiO-66 doped with LiOtBu have been developed that capture and hydrolytically detoxify simulants of nerve agents and mustard gas. Both MOFs function as highly catalytic elements in these applications.}, language = {en} } @misc{WippertPuschmannSchiltenwolfetal.2016, author = {Wippert, Pia-Maria and Puschmann, Anne-Katrin and Schiltenwolf, Marcus and Wiebking, Christine and Mayer, Frank}, title = {BACK PAIN: THE STUDY OF MECHANISMS AND THE TRANSLATION IN INTERVENTIONS WITHIN THE MISPEX NETWORK}, series = {Psychosomatic medicine}, volume = {78}, journal = {Psychosomatic medicine}, publisher = {Elsevier}, address = {Philadelphia}, issn = {0033-3174}, pages = {A91 -- A91}, year = {2016}, language = {en} } @misc{PassarelliHainzlCescaetal.2016, author = {Passarelli, Luigi and Hainzl, Sebastian and Cesca, Simone and Maccaferri, Francesco and Mucciarelli, Marco and Roessler, Dirk and Corbi, Fabio and Dahm, Torsten and Rivalta, Eleonora}, title = {Aseismic transient driving the swarm-like seismic sequence in the Pollino range, Southern Italy (vol 201, pg 1553, 2015)}, series = {Geophysical journal international}, volume = {204}, journal = {Geophysical journal international}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {0956-540X}, doi = {10.1093/gji/ggv425}, pages = {365 -- 365}, year = {2016}, language = {en} } @misc{Gruene2016, author = {Gr{\"u}ne, Stefanie}, title = {Allais on Intuitions and the Objective Reality of the Categories}, series = {European journal of philosophy}, volume = {24}, journal = {European journal of philosophy}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {0966-8373}, doi = {10.1111/ejop.12139}, pages = {241 -- 252}, year = {2016}, language = {en} } @misc{HeinzBeckRapp2016, author = {Heinz, Andreas and Beck, Anne and Rapp, Michael A.}, title = {Alcohol as an Environmental Mortality Hazard}, series = {JAMA psychiatry}, volume = {73}, journal = {JAMA psychiatry}, publisher = {American Veterinary Medical Association}, address = {Chicago}, issn = {2168-622X}, doi = {10.1001/jamapsychiatry.2016.0399}, pages = {549 -- 550}, year = {2016}, language = {en} } @misc{EhmerBarthWeingarten2016, author = {Ehmer, Oliver and Barth-Weingarten, Dagmar}, title = {Adverbial patterns in interaction}, series = {Language sciences}, volume = {58}, journal = {Language sciences}, publisher = {Elsevier}, address = {Oxford}, issn = {0388-0001}, doi = {10.1016/j.langsci.2016.05.001}, pages = {1 -- 7}, year = {2016}, language = {en} } @misc{BuergerMagdansGies2016, author = {B{\"u}rger, Andreas and Magdans, Uta and Gies, Hermann}, title = {Adsorption of amino acids on the magnetite-(111)-surface: a force field study (vol 19, 851, 2013)}, series = {Journal of molecular modeling}, volume = {22}, journal = {Journal of molecular modeling}, publisher = {Springer}, address = {New York}, issn = {1610-2940}, doi = {10.1007/s00894-016-3124-8}, pages = {3}, year = {2016}, language = {en} } @misc{LevermannPetoukhovScheweetal.2016, author = {Levermann, Anders and Petoukhov, Vladimir and Schewe, Jacob and Schellnhuber, Hans Joachim}, title = {Abrupt monsoon transitions as seen in paleorecords can be explained by moisture-advection feedback}, series = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {113}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, publisher = {National Acad. of Sciences}, address = {Washington}, issn = {0027-8424}, doi = {10.1073/pnas.1603130113}, pages = {E2348 -- E2349}, year = {2016}, language = {en} } @misc{PuschmannWippert2016, author = {Puschmann, Anne-Katrin and Wippert, Pia-Maria}, title = {A LONGITUDINAL INTERDISCIPLINARY APPROACH TO STRESS AND ITS IMPACT ON THE DEVELOPMENT OF CHRONIC PAIN}, series = {Psychosomatic medicine}, volume = {78}, journal = {Psychosomatic medicine}, publisher = {Routledge, Taylor \& Francis Group}, address = {Philadelphia}, issn = {0033-3174}, pages = {A91 -- A91}, year = {2016}, language = {en} } @misc{Esguerra2016, author = {Esguerra, Alejandro}, title = {"A Comment That Might Help Us to Move Along"}, series = {Sustainability Politics and Limited Statehood : Contesting the New Modes of Governance}, journal = {Sustainability Politics and Limited Statehood : Contesting the New Modes of Governance}, publisher = {Cham}, address = {Basingstoke}, isbn = {978-3-319-39871-6}, doi = {10.1007/978-3-319-39871-6_2}, pages = {25 -- 46}, year = {2016}, abstract = {This chapter investigates the trajectory of establishing the Forest Stewardship Council (FSC) in the early 1990s as the first private transnational certification organization with an antagonistic stakeholder body. Its main contribution is a micro-analysis of the founding assembly in 1993. By investigating the role of brokers within the negotiation as one institutional scope condition for 'arguing' having occurred, the chapter adopts a dramaturgical approach. It contends that the authority of brokers is not necessarily institutionally given, but needs to be gained: brokers have to prove situationally that their knowledge is relevant and that they are speaking impartially in the interest of progress rather than their own. The chapter stresses the importance of procedural knowledge which brokers provide in contrast to policy knowledge.}, language = {en} }