@article{YadavalliLoebnerPapkeetal.2016, author = {Yadavalli, Nataraja Sekhar and Loebner, Sarah and Papke, Thomas and Sava, Elena and Hurduc, Nicolae and Santer, Svetlana}, title = {A comparative study of photoinduced deformation in azobenzene containing polymer films}, series = {Soft matter}, volume = {12}, journal = {Soft matter}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {1744-683X}, doi = {10.1039/c6sm00029k}, pages = {2593 -- 2603}, year = {2016}, abstract = {In this paper two groups supporting different views on the mechanism of light induced polymer deformation argue about the respective underlying theoretical conceptions, in order to bring this interesting debate to the attention of the scientific community. The group of Prof. Nicolae Hurduc supports the model claiming that the cyclic isomerization of azobenzenes may cause an athermal transition of the glassy azobenzene containing polymer into a fluid state, the so-called photo-fluidization concept. This concept is quite convenient for an intuitive understanding of the deformation process as an anisotropic flow of the polymer material. The group of Prof. Svetlana Santer supports the re-orientational model where the mass-transport of the polymer material accomplished during polymer deformation is stated to be generated by the light-induced re-orientation of the azobenzene side chains and as a consequence of the polymer backbone that in turn results in local mechanical stress, which is enough to irreversibly deform an azobenzene containing material even in the glassy state. For the debate we chose three polymers differing in the glass transition temperature, 32 degrees C, 87 degrees C and 95 degrees C, representing extreme cases of flexible and rigid materials. Polymer film deformation occurring during irradiation with different interference patterns is recorded using a homemade set-up combining an optical part for the generation of interference patterns and an atomic force microscope for acquiring the kinetics of film deformation. We also demonstrated the unique behaviour of azobenzene containing polymeric films to switch the topography in situ and reversibly by changing the irradiation conditions. We discuss the results of reversible deformation of three polymers induced by irradiation with intensity (IIP) and polarization (PIP) interference patterns, and the light of homogeneous intensity in terms of two approaches: the re-orientational and the photo-fluidization concepts. Both agree in that the formation of opto-mechanically induced stresses is a necessary prerequisite for the process of deformation. Using this argument, the deformation process can be characterized either as a flow or mass transport.}, language = {en} } @article{PatilHanneBurchertetal.2016, author = {Patil, Umesh and Hanne, Sandra and Burchert, Frank and De Bleser, Ria and Vasishth, Shravan}, title = {A Computational Evaluation of Sentence Processing Deficits in Aphasia}, series = {Cognitive science : a multidisciplinary journal of anthropology, artificial intelligence, education, linguistics, neuroscience, philosophy, psychology ; journal of the Cognitive Science Society}, volume = {40}, journal = {Cognitive science : a multidisciplinary journal of anthropology, artificial intelligence, education, linguistics, neuroscience, philosophy, psychology ; journal of the Cognitive Science Society}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {0364-0213}, doi = {10.1111/cogs.12250}, pages = {5 -- 50}, year = {2016}, abstract = {Individuals with agrammatic Broca's aphasia experience difficulty when processing reversible non-canonical sentences. Different accounts have been proposed to explain this phenomenon. The Trace Deletion account (Grodzinsky, 1995, 2000, 2006) attributes this deficit to an impairment in syntactic representations, whereas others (e.g., Caplan, Waters, Dede, Michaud, \& Reddy, 2007; Haarmann, Just, \& Carpenter, 1997) propose that the underlying structural representations are unimpaired, but sentence comprehension is affected by processing deficits, such as slow lexical activation, reduction in memory resources, slowed processing and/or intermittent deficiency, among others. We test the claims of two processing accounts, slowed processing and intermittent deficiency, and two versions of the Trace Deletion Hypothesis (TDH), in a computational framework for sentence processing (Lewis \& Vasishth, 2005) implemented in ACT-R (Anderson, Byrne, Douglass, Lebiere, \& Qin, 2004). The assumption of slowed processing is operationalized as slow procedural memory, so that each processing action is performed slower than normal, and intermittent deficiency as extra noise in the procedural memory, so that the parsing steps are more noisy than normal. We operationalize the TDH as an absence of trace information in the parse tree. To test the predictions of the models implementing these theories, we use the data from a German sentence—picture matching study reported in Hanne, Sekerina, Vasishth, Burchert, and De Bleser (2011). The data consist of offline (sentence-picture matching accuracies and response times) and online (eye fixation proportions) measures. From among the models considered, the model assuming that both slowed processing and intermittent deficiency are present emerges as the best model of sentence processing difficulty in aphasia. The modeling of individual differences suggests that, if we assume that patients have both slowed processing and intermittent deficiency, they have them in differing degrees.}, language = {en} } @article{Dulgheru2016, author = {Dulgheru, Elena}, title = {A Conjunction of Mysteries}, series = {Andrej Tarkovskij: Klassiker - Классик - Classic - Classico : Beitr{\"a}ge zum internationalen Tarkovskij-Symposium an der Universit{\"a}t Potsdam ; Band 1}, journal = {Andrej Tarkovskij: Klassiker - Классик - Classic - Classico : Beitr{\"a}ge zum internationalen Tarkovskij-Symposium an der Universit{\"a}t Potsdam ; Band 1}, publisher = {Universit{\"a}t Potsdam}, address = {Potsdam}, isbn = {978-3-86956-351-0}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-95594}, pages = {201 -- 214}, year = {2016}, language = {en} } @article{Leung2016, author = {Leung, Ray C. H.}, title = {A corpus-based analysis of textbooks used in the orientation course for immigrants in Germany: Ideological and pedagogic implications}, series = {Journal of Language and Cultural Education}, volume = {4}, journal = {Journal of Language and Cultural Education}, publisher = {De Gruyter}, address = {Berlin}, issn = {1339-4045}, doi = {10.1515/jolace-2016-0030}, pages = {154 -- 177}, year = {2016}, language = {en} } @article{WangYangMeinel2016, author = {Wang, Cheng and Yang, Haojin and Meinel, Christoph}, title = {A deep semantic framework for multimodal representation learning}, series = {Multimedia tools and applications : an international journal}, volume = {75}, journal = {Multimedia tools and applications : an international journal}, publisher = {Springer}, address = {Dordrecht}, issn = {1380-7501}, doi = {10.1007/s11042-016-3380-8}, pages = {9255 -- 9276}, year = {2016}, abstract = {Multimodal representation learning has gained increasing importance in various real-world multimedia applications. Most previous approaches focused on exploring inter-modal correlation by learning a common or intermediate space in a conventional way, e.g. Canonical Correlation Analysis (CCA). These works neglected the exploration of fusing multiple modalities at higher semantic level. In this paper, inspired by the success of deep networks in multimedia computing, we propose a novel unified deep neural framework for multimodal representation learning. To capture the high-level semantic correlations across modalities, we adopted deep learning feature as image representation and topic feature as text representation respectively. In joint model learning, a 5-layer neural network is designed and enforced with a supervised pre-training in the first 3 layers for intra-modal regularization. The extensive experiments on benchmark Wikipedia and MIR Flickr 25K datasets show that our approach achieves state-of-the-art results compare to both shallow and deep models in multimodal and cross-modal retrieval.}, language = {en} } @article{JambrinaEnriquezSachseValeroGarces2016, author = {Jambrina-Enriquez, Margarita and Sachse, Dirk and Valero-Garces, Blas L.}, title = {A deglaciation and Holocene biomarker-based reconstruction of climate and environmental variability in NW Iberian Peninsula: the Sanabria Lake sequence}, series = {Journal of paleolimnolog}, volume = {56}, journal = {Journal of paleolimnolog}, publisher = {Springer}, address = {Dordrecht}, issn = {0921-2728}, doi = {10.1007/s10933-016-9890-6}, pages = {49 -- 66}, year = {2016}, abstract = {The molecular biomarker composition of two sediment cores from Sanabria Lake (NW Iberian Peninsula) and a survey of modern plants in the watershed provide a reconstruction of past vegetation and landscape dynamics since deglaciation. During a proglacial stage in Lake Sanabria (prior to 14.7 cal ka BP), very low biomarker concentration and carbon preference index (CPI) values similar to 1 suggest that the n-alkanes could have derived from eroded ancient sediment sources or older organic matter with high degree of maturity. During the Late glacial (14.7-11.7 cal ka BP) and the Holocene (last 11.7 cal ka BP) intervals with higher biomarker and triterpenoid concentrations (high \%nC(29) , nC(31) alkanes), higher CPI and average carbon length (ACL), and lower P-aq (proportion of aquatic plants) are indicative of major contribution of vascular land plants from a more forested watershed (e.g. Mid Holocene period 7.0-4.0 cal ka BP). Lower biomarker concentrations (high \%nC(27) alkanes), CPI and ACL values responded to short phases with decreased allochthonous contribution into the lake that correspond to centennial-scale periods of regional forest decline (e.g. 4-3 ka BP, Roman deforestation after 2.0 ka, and some phases of the LIA, seventeenth-nineteenth centuries). Human activities in the watershed were significant during early medieval times (1.3-1.0 cal ka BP) and since 1960 CE, in both cases associated with relatively higher productivity stages in the lake (lower biomarker and triterpenoid concentrations, high \%nC(23) and \%nC(31) respectively, lower ACL and CPI values and higher P-aq). The lipid composition of Sanabria Lake sediments indicates a major allochthonous (watershed-derived) contribution to the organic matter budget since deglaciation, and a dominant oligotrophic status during the lake history. The study constrains the climate and anthropogenic forcings and watershed versus lake sources in organic matter accumulation processes and helps to design conservation and management policies in mountain, oligotrophic lakes.}, language = {en} } @article{EhlertHolzweberLippitzetal.2016, author = {Ehlert, Christopher and Holzweber, Markus and Lippitz, Andreas and Unger, Wolfgang E. S. and Saalfrank, Peter}, title = {A detailed assignment of NEXAFS resonances of imidazolium based ionic liquids}, series = {Physical chemistry, chemical physics : a journal of European Chemical Societies}, volume = {18}, journal = {Physical chemistry, chemical physics : a journal of European Chemical Societies}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {1463-9076}, doi = {10.1039/c5cp07434g}, pages = {8654 -- 8661}, year = {2016}, abstract = {In Near Edge X-Ray Absorption Fine Structure (NEXAFS) spectroscopy X-Ray photons are used to excite tightly bound core electrons to low-lying unoccupied orbitals of the system. This technique offers insight into the electronic structure of the system as well as useful structural information. In this work, we apply NEXAFS to two kinds of imidazolium based ionic liquids ([C(n)C(1)im](+)[NTf2](-) and [C(4)C(1)im](+)[I](-)). A combination of measurements and quantum chemical calculations of C K and N K NEXAFS resonances is presented. The simulations, based on the transition potential density functional theory method (TP-DFT), reproduce all characteristic features observed by the experiment. Furthermore, a detailed assignment of resonance features to excitation centers (carbon or nitrogen atoms) leads to a consistent interpretation of the spectra.}, language = {en} } @misc{EhlertHolzweberLippitzetal.2016, author = {Ehlert, Christopher and Holzweber, Markus and Lippitz, Andreas and Unger, Wolfgang E. S. and Saalfrank, Peter}, title = {A detailed assignment of NEXAFS resonances of imidazolium based ionic liquids}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-394417}, pages = {8654 -- 8661}, year = {2016}, abstract = {In Near Edge X-Ray Absorption Fine Structure (NEXAFS) spectroscopy X-Ray photons are used to excite tightly bound core electrons to low-lying unoccupied orbitals of the system. This technique offers insight into the electronic structure of the system as well as useful structural information. In this work, we apply NEXAFS to two kinds of imidazolium based ionic liquids ([CnC1im]+[NTf2]- and [C4C1im]+[I]-). A combination of measurements and quantum chemical calculations of C K and N K NEXAFS resonances is presented. The simulations, based on the transition potential density functional theory method (TP-DFT), reproduce all characteristic features observed by the experiment. Furthermore, a detailed assignment of resonance features to excitation centers (carbon or nitrogen atoms) leads to a consistent interpretation of the spectra.}, language = {en} } @article{SchlaegelLewis2016, author = {Schl{\"a}gel, Ulrike E. and Lewis, Mark A.}, title = {A framework for analyzing the robustness of movement models to variable step discretization}, series = {Journal of mathematical biology}, volume = {73}, journal = {Journal of mathematical biology}, publisher = {Springer}, address = {Heidelberg}, issn = {0303-6812}, doi = {10.1007/s00285-016-0969-5}, pages = {815 -- 845}, year = {2016}, abstract = {When sampling animal movement paths, the frequency at which location measurements are attempted is a critical feature for data analysis. Important quantities derived from raw data, e.g. travel distance or sinuosity, can differ largely based on the temporal resolution of the data. Likewise, when movement models are fitted to data, parameter estimates have been demonstrated to vary with sampling rate. Thus, biological statements derived from such analyses can only be made with respect to the resolution of the underlying data, limiting extrapolation of results and comparison between studies. To address this problem, we investigate whether there are models that are robust against changes in temporal resolution. First, we propose a mathematically rigorous framework, in which we formally define robustness as a model property. We then use the framework for a thorough assessment of a range of basic random walk models, in which we also show how robustness relates to other probabilistic concepts. While we found robustness to be a strong condition met by few models only, we suggest a new method to extend models so as to make them robust. Our framework provides a new systematic, mathematically founded approach to the question if, and how, sampling rate of movement paths affects statistical inference.}, language = {en} } @misc{MaoNakamuraViottietal.2016, author = {Mao, Hailiang and Nakamura, Moritaka and Viotti, Corrado and Grebe, Markus}, title = {A framework for lateral membrane trafficking and polar tethering of the PEN3 ATP-Binding cassette transporter}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {909}, issn = {1866-8372}, doi = {10.25932/publishup-44130}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-441302}, pages = {2245 -- 2260}, year = {2016}, abstract = {The outermost cell layer of plants, the epidermis, and its outer (lateral) membrane domain facing the environment are continuously challenged by biotic and abiotic stresses. Therefore, the epidermis and the outer membrane domain provide important selective and protective barriers. However, only a small number of specifically outer membrane-localized proteins are known. Similarly, molecular mechanisms underlying the trafficking and the polar placement of outer membrane domain proteins require further exploration. Here, we demonstrate that ACTIN7 (ACT7) mediates trafficking of the PENETRATION3 (PEN3) outer membrane protein from the trans-Golgi network (TGN) to the plasma membrane in the root epidermis of Arabidopsis (Arabidopsis thaliana) and that actin function contributes to PEN3 endocytic recycling. In contrast to such generic ACT7-dependent trafficking from the TGN, the EXOCYST84b (EXO84b) tethering factor mediates PEN3 outer-membrane polarity. Moreover, precise EXO84b placement at the outer membrane domain itself requires ACT7 function. Hence, our results uncover spatially and mechanistically distinct requirements for ACT7 function during outer lateral membrane cargo trafficking and polarity establishment. They further identify an exocyst tethering complex mediator of outer lateral membrane cargo polarity.}, language = {en} } @article{MaoNakamuraViottietal.2016, author = {Mao, Hailiang and Nakamura, Moritaka and Viotti, Corrado and Grebe, Markus}, title = {A Framework for Lateral Membrane Trafficking and Polar Tethering of the PEN3 ATP-Binding Cassette Transporter}, series = {Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants}, volume = {172}, journal = {Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants}, publisher = {American Society of Plant Physiologists}, address = {Rockville}, issn = {0032-0889}, doi = {10.1104/pp.16.01252}, pages = {2245 -- 2260}, year = {2016}, abstract = {The outermost cell layer of plants, the epidermis, and its outer (lateral) membrane domain facing the environment are continuously challenged by biotic and abiotic stresses. Therefore, the epidermis and the outer membrane domain provide important selective and protective barriers. However, only a small number of specifically outer membrane-localized proteins are known. Similarly, molecular mechanisms underlying the trafficking and the polar placement of outer membrane domain proteins require further exploration. Here, we demonstrate that ACTIN7 (ACT7) mediates trafficking of the PENETRATION3 (PEN3) outer membrane protein from the trans-Golgi network (TGN) to the plasma membrane in the root epidermis of Arabidopsis (Arabidopsis thaliana) and that actin function contributes to PEN3 endocytic recycling. In contrast to such generic ACT7-dependent trafficking from the TGN, the EXOCYST84b (EXO84b) tethering factor mediates PEN3 outer-membrane polarity. Moreover, precise EXO84b placement at the outer membrane domain itself requires ACT7 function. Hence, our results uncover spatially and mechanistically distinct requirements for ACT7 function during outer lateral membrane cargo trafficking and polarity establishment. They further identify an exocyst tethering complex mediator of outer lateral membrane cargo polarity.}, language = {en} } @article{CollenburgWalterBurgertetal.2016, author = {Collenburg, Lena and Walter, Tim and Burgert, Anne and Mueller, Nora and Seibel, Juergen and Japtok, Lukasz and Kleuser, Burkhard and Sauer, Markus and Schneider-Schaulies, Sibylle}, title = {A Functionalized Sphingolipid Analogue for Studying Redistribution during Activation in Living T Cells}, series = {The journal of immunology}, volume = {196}, journal = {The journal of immunology}, publisher = {American Assoc. of Immunologists}, address = {Bethesda}, issn = {0022-1767}, doi = {10.4049/jimmunol.1502447}, pages = {3951 -- 3962}, year = {2016}, abstract = {Sphingolipids are major components of the plasma membrane. In particular, ceramide serves as an essential building hub for complex sphingolipids, but also as an organizer of membrane domains segregating receptors and signalosomes. Sphingomyelin breakdown as a result of sphingomyelinase activation after ligation of a variety of receptors is the predominant source of ceramides released at the plasma membrane. This especially applies to T lymphocytes where formation of ceramide-enriched membrane microdomains modulates TCR signaling. Because ceramide release and redistribution occur very rapidly in response to receptor ligation, novel tools to further study these processes in living T cells are urgently needed. To meet this demand, we synthesized nontoxic, azido-functionalized ceramides allowing for bio-orthogonal click-reactions to fluorescently label incorporated ceramides, and thus investigate formation of ceramide-enriched domains. Azido-functionalized C-6-ceramides were incorporated into and localized within plasma membrane microdomains and proximal vesicles in T cells. They segregated into clusters after TCR, and especially CD28 ligation, indicating efficient sorting into plasma membrane domains associated with T cell activation; this was abolished upon sphingomyelinase inhibition. Importantly, T cell activation was not abrogated upon incorporation of the compound, which was efficiently excluded from the immune synapse center as has previously been seen in Ab-based studies using fixed cells. Therefore, the functionalized ceramides are novel, highly potent tools to study the subcellular redistribution of ceramides in the course of T cell activation. Moreover, they will certainly also be generally applicable to studies addressing rapid stimulation-mediated ceramide release in living cells.}, language = {en} } @misc{LiaimerJensenDittmannThuenemann2016, author = {Liaimer, Anton and Jensen, John B. and Dittmann-Th{\"u}nemann, Elke}, title = {A genetic and chemical perspective on symbiotic recruitment of cyanobacteria of the genus Nostoc into the host plant Blasia pusilla L.}, series = {Frontiers in microbiology}, journal = {Frontiers in microbiology}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-407179}, pages = {16}, year = {2016}, abstract = {Liverwort Blasia pusilla L. recruits soil nitrogen-fixing cyanobacteria of genus Nostoc as symbiotic partners. In this work we compared Nostoc community composition inside the plants and in the soil around them from two distant locations in Northern Norway. STRR fingerprinting and 16S rDNA phylogeny reconstruction showed a remarkable local diversity among isolates assigned to several Nostoc clades. An extensive web of negative allelopathic interactions was recorded at an agricultural site, but not at the undisturbed natural site. The cell extracts of the cyanobacteria did not show antimicrobial activities, but four isolates were shown to be cytotoxic to human cells. The secondary metabolite profiles of the isolates were mapped by MALDI-TOF MS, and the most prominent ions were further analyzed by Q-TOF for MS/MS aided identification. Symbiotic isolates produced a great variety of small peptide-like substances, most of which lack any record in the databases. Among identified compounds we found microcystin and nodularin variants toxic to eukaryotic cells. Microcystin producing chemotypes were dominating as symbiotic recruits but not in the free-living community. In addition, we were able to identify several novel aeruginosins and banyaside-like compounds, as well as nostocyclopeptides and nosperin.}, language = {en} } @article{LiaimerJensenDittmann2016, author = {Liaimer, Anton and Jensen, John B. and Dittmann, Elke}, title = {A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L.}, series = {Frontiers in microbiology}, volume = {7}, journal = {Frontiers in microbiology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-302X}, doi = {10.3389/fmicb.2016.01693}, pages = {449 -- 474}, year = {2016}, abstract = {Liverwort Blasia pusilla L. recruits soil nitrogen-fixing cyanobacteria of genus Nostoc as symbiotic partners. In this work we compared Nostoc community composition inside the plants and in the soil around them from two distant locations in Northern Norway. STRR fingerprinting and 16S rDNA phylogeny reconstruction showed a remarkable local diversity among isolates assigned to several Nostoc clades. An extensive web of negative allelopathic interactions was recorded at an agricultural site, but not at the undisturbed natural site. The cell extracts of the cyanobacteria did not show antimicrobial activities, but four isolates were shown to be cytotoxic to human cells. The secondary metabolite profiles of the isolates were mapped by MALDI-TOF MS, and the most prominent ions were further analyzed by Q-TOF for MS/MS aided identification. Symbiotic isolates produced a great variety of small peptide-like substances, most of which lack any record in the databases. Among identified compounds we found microcystin and nodularin variants toxic to eukaryotic cells. Microcystin producing chemotypes were dominating as symbiotic recruits but not in the free-living community. In addition, we were able to identify several novel aeruginosins and banyaside-like compounds, as well as nostocyclopeptides and nosperin.}, language = {en} } @article{AndersMefenzaBobdaetal.2016, author = {Anders, Jakob and Mefenza, Michael and Bobda, Christophe and Yonga, Franck and Aklah, Zeyad and Gunn, Kevin}, title = {A hardware/software prototyping system for driving assistance investigations}, series = {Journal of real-time image processing}, volume = {11}, journal = {Journal of real-time image processing}, publisher = {Springer}, address = {Heidelberg}, issn = {1861-8200}, doi = {10.1007/s11554-013-0351-4}, pages = {559 -- 569}, year = {2016}, abstract = {A holistic design and verification environment to investigate driving assistance systems is presented, with an emphasis on system-on-chip architectures for video applications. Starting with an executable specification of a driving assistance application, subsequent transformations are performed across different levels of abstraction until the final implementation is achieved. The hardware/software partitioning is facilitated through the integration of OpenCV and SystemC in the same design environment, as well as OpenCV and Linux in the run-time system. We built a rapid prototyping, FPGA-based camera system, which allows designs to be explored and evaluated in realistic conditions. Using lane departure and the corresponding performance speedup, we show that our platform reduces the design time, while improving the verification efforts.}, language = {en} } @article{SchwarzeSchneiderRiemeretal.2016, author = {Schwarze, Thomas and Schneider, Radu and Riemer, Janine and Holdt, Hans-J{\"u}rgen}, title = {A Highly K+-Selective Fluorescent Probe - Tuning the K+-Complex Stability and the K+/Na+ Selectivity by Varying the Lariat-Alkoxy Unit of a Phenylaza[18]crown-6 Ionophore}, series = {Chemistry : an Asian journal ; an ACES journal}, volume = {11}, journal = {Chemistry : an Asian journal ; an ACES journal}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1861-4728}, doi = {10.1002/asia.201500956}, pages = {241 -- 247}, year = {2016}, abstract = {A desirable goal is to synthesize easily accessible and highly K+/Na+-selective fluoroionophores to monitor physiological K+ levels in vitro and in vivo. Therefore, highly K+/Na+-selective ionophores have to be developed. Herein, we obtained in a sequence of only four synthetic steps a set of K+-responsive fluorescent probes 4, 5 and 6. In a systematic study, we investigated the influence of the alkoxy substitution in ortho position of the aniline moiety in -conjugated aniline-1,2,3-triazole-coumarin-fluoroionophores 4, 5 and 6 [R=MeO (4), EtO (5) and iPrO (6)] towards the K+-complex stability and K+/Na+ selectivity. The highest K+-complex stability showed fluoroionophore 4 with a dissociation constant K-d of 19mm, but the K-d value increases to 31mm in combined K+/Na+ solutions, indicating a poor K+/Na+ selectivity. By contrast, 6 showed even in the presence of competitive Na+ ions equal K-d values (K-d(K+)=45mm and K-d(K+/Na+)=45mm) and equal K+-induced fluorescence enhancement factors (FEFs=2.3). Thus, the fluorescent probe 6 showed an outstanding K+/Na+ selectivity and is a suitable fluorescent tool to measure physiological K+ levels in the range of 10-80mm in vitro. Further, the isopropoxy-substituted N-phenylaza[18]crown-6 ionophore in 6 is a highly K+-selective building block with a feasible synthetic route.}, language = {en} } @unpublished{AlsaedyTarkhanov2016, author = {Alsaedy, Ammar and Tarkhanov, Nikolai Nikolaevich}, title = {A Hilbert boundary value problem for generalised Cauchy-Riemann equations}, volume = {5}, number = {1}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {2193-6943}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-86109}, pages = {21}, year = {2016}, abstract = {We elaborate a boundary Fourier method for studying an analogue of the Hilbert problem for analytic functions within the framework of generalised Cauchy-Riemann equations. The boundary value problem need not satisfy the Shapiro-Lopatinskij condition and so it fails to be Fredholm in Sobolev spaces. We show a solvability condition of the Hilbert problem, which looks like those for ill-posed problems, and construct an explicit formula for approximate solutions.}, language = {en} } @article{LaemkeBrzezinkaAltmannetal.2016, author = {L{\"a}mke, J{\"o}rn and Brzezinka, Krzysztof and Altmann, Simone and B{\"a}urle, Isabel}, title = {A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory}, series = {The EMBO journal}, volume = {35}, journal = {The EMBO journal}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {0261-4189}, doi = {10.15252/embj.201592593}, pages = {162 -- 175}, year = {2016}, abstract = {In nature, plants often encounter chronic or recurring stressful conditions. Recent results indicate that plants can remember a past exposure to stress to be better prepared for a future stress incident. However, the molecular basis of this is poorly understood. Here, we report the involvement of chromatin modifications in the maintenance of acquired thermotolerance (heat stress [HS] memory). HS memory is associated with the accumulation of histone H3 lysine 4 di- and trimethylation at memory-related loci. This accumulation outlasts their transcriptional activity and marks them as recently transcriptionally active. High accumulation of H3K4 methylation is associated with hyper-induction of gene expression upon a recurring HS. This transcriptional memory and the sustained accumulation of H3K4 methylation depend on HSFA2, a transcription factor that is required for HS memory, but not initial heat responses. Interestingly, HSFA2 associates with memory-related loci transiently during the early stages following HS. In summary, we show that transcriptional memory after HS is associated with sustained H3K4 hyper-methylation and depends on a hit-and-run transcription factor, thus providing a molecular framework for HS memory.}, language = {en} } @article{KabelitzBrzezinkaFriedrichetal.2016, author = {Kabelitz, Tina and Brzezinka, Krzysztof and Friedrich, Thomas and Gorka, Michal and Graf, Alexander and Kappel, Christian and B{\"a}urle, Isabel}, title = {A JUMONJI Protein with E3 Ligase and Histone H3 Binding Activities Affects Transposon Silencing in Arabidopsis}, series = {Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants}, volume = {171}, journal = {Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants}, publisher = {American Society of Plant Physiologists}, address = {Rockville}, issn = {0032-0889}, doi = {10.1104/pp.15.01688}, pages = {344 -- 358}, year = {2016}, abstract = {Transposable elements (TEs) make up a large proportion of eukaryotic genomes. As their mobilization creates genetic variation that threatens genome integrity, TEs are epigenetically silenced through several pathways, and this may spread to neighboring sequences. JUMONJI (JMJ) proteins can function as antisilencing factors and prevent silencing of genes next to TEs. Whether TE silencing is counterbalanced by the activity of antisilencing factors is still unclear. Here, we characterize JMJ24 as a regulator of TE silencing. We show that loss of JMJ24 results in increased silencing of the DNA transposon AtMu1c, while overexpression of JMJ24 reduces silencing. JMJ24 has a JumonjiC (JmjC) domain and two RING domains. JMJ24 autoubiquitinates in vitro, demonstrating E3 ligase activity of the RING domain(s). JMJ24-JmjC binds the N-terminal tail of histone H3, and full-length JMJ24 binds histone H3 in vivo. JMJ24 activity is anticorrelated with histone H3 Lys 9 dimethylation (H3K9me2) levels at AtMu1c. Double mutant analyses with epigenetic silencing mutants suggest that JMJ24 antagonizes histone H3K9me2 and requires H3K9 methyltransferases for its activity on AtMu1c. Genome-wide transcriptome analysis indicates that JMJ24 affects silencing at additional TEs. Our results suggest that the JmjC domain of JMJ24 has lost demethylase activity but has been retained as a binding domain for histone H3. This is in line with phylogenetic analyses indicating that JMJ24 (with the mutated JmjC domain) is widely conserved in angiosperms. Taken together, this study assigns a role in TE silencing to a conserved JmjC-domain protein with E3 ligase activity, but no demethylase activity.}, language = {en} } @misc{PuschmannWippert2016, author = {Puschmann, Anne-Katrin and Wippert, Pia-Maria}, title = {A LONGITUDINAL INTERDISCIPLINARY APPROACH TO STRESS AND ITS IMPACT ON THE DEVELOPMENT OF CHRONIC PAIN}, series = {Psychosomatic medicine}, volume = {78}, journal = {Psychosomatic medicine}, publisher = {Routledge, Taylor \& Francis Group}, address = {Philadelphia}, issn = {0033-3174}, pages = {A91 -- A91}, year = {2016}, language = {en} }