@misc{ZiegeMahlowHennigeSulzetal.2009,
  author    = {Ziege, Madlen and Mahlow, Kristin and Hennige-Sulz, Carmen and Kronmarck, Claudia and Tiedemann, Ralph and Streit, Bruno and Plath, Martin},
  title     = {Audience effects in the Atlantic molly (Poecilia mexicana) : prudent male mate choice in response to perceived sperm competition risk?},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-45171},
  year      = {2009},
  abstract  = {Background: Multidirectional interactions in social networks can have a profound effect on mate choice behavior; e.g., Poecilia mexicana males show weaker expression of mating preferences when being observed by a rival. This may be an adaptation to reduce sperm competition risk, which arises because commonly preferred female phenotypes will receive attention also from surrounding males, and/or because other males can copy the focal male's mate choice. Do P. mexicana males indeed respond to perceived sperm competition risk? We gave males a choice between two females and repeated the tests under one of the following conditions: (1) an empty transparent cylinder was presented (control); (2) another ("audience") male inside the cylinder observed the focal male throughout the 2nd part, or (3) the audience male was presented only before the tests, but could not eavesdrop during the actual choice tests (non-specific sperm competition risk treatments); (4) the focal male could see a rival male interact sexually with the previously preferred, or (5) with the non-preferred female before the 2nd part of the tests (specific sperm competition risk treatments). Results: The strength of individual male preferences declined slightly also during the control treatment (1). However, this decrease was more than two-fold stronger in audience treatment (2), i.e., with non-specific sperm competition risk including the possibility for visual eavesdropping by the audience male. No audience effect was found in treatments (3) and (5), but a weak effect was also observed when the focal male had seen the previously preferred female sexually interact with a rival male (treatment 4; specific sperm competition risk). Conclusion: When comparing the two 'non-specific sperm competition risk' treatments, a very strong effect was found only when the audience male could actually observe the focal male during mate choice [treatment (2)]. This suggests that focal males indeed attempt to conceal their mating preferences so as to prevent surrounding males from copying their mate choice. When there is no potential for eavesdropping [treatment (3)], non-specific specific sperm competition risk seems to play a minor or no role. Our results also show that P. mexicana males tend to share their mating effort more equally among females when the resource value of their previously preferred mate decreases after mating with a rival male (perceived specific sperm competition risk), but this effect is comparatively weak.},
  language  = {en}
}
@article{ZiegeHennigeSchulzMueckschetal.2012,
  author    = {Ziege, Madlen and Hennige-Schulz, Carmen and Muecksch, Frauke and Bierbach, David and Tiedemann, Ralph and Streit, Bruno and Plath, Martin},
  title     = {A comparison of two methods to assess audience-induced changes in male mate choice},
  series = {Current zoology},
  volume    = {58},
  journal   = {Current zoology},
  number    = {1},
  publisher = {Current Zoology},
  address   = {Beijing},
  issn      = {1674-5507},
  pages     = {84 -- 94},
  year      = {2012},
  abstract  = {Multidirectional communicative interactions in social networks can have a profound effect on mate choice behavior. Male Atlantic molly Poecilia mexicana exhibit weaker mating preferences when an audience male is presented. This could be a male strategy to reduce sperm competition risk: interacting more equally with different females may be advantageous because rivals might copy mate choice decisions. In line with this hypothesis, a previous study found males to show a strong audience effect when being observed while exercising mate choice, but not when the rival was presented only before the choice tests. Audience effects on mate choice decisions have been quantified in poeciliid fishes using association preference designs, but it remains unknown if patterns found from measuring association times translate into actual mating behavior. Thus, we created five audience treatments simulating different forms of perceived sperm competition risk and determined focal males' mating preferences by scoring pre-mating (nipping) and mating behavior (gonopodial thrusting). Nipping did not reflect the pattern that was found when association preferences were measured, while a very similar pattern was uncovered in thrusting behavior. The strongest response was observed when the audience could eavesdrop on the focal male's behavior. A reduction in the strength of focal males' preferences was also seen after the rival male had an opportunity to mate with the focal male's preferred mate. In comparison, the reduction of mating preferences in response to an audience was greater when measuring association times than actual mating behavior. While measuring direct sexual interactions between the focal male and both stimulus females not only the male's motivational state is reflected but also females' behavior such as avoidance of male sexual harassment.},
  language  = {en}
}
@article{ZhuSchluppTiedemann2017,
  author    = {Zhu, Fangjun and Schlupp, Ingo and Tiedemann, Ralph},
  title     = {Allele-specific expression at the androgen receptor alpha gene in a hybrid unisexual fish, the Amazon molly (Poecilia formosa)},
  series = {PLoS one},
  volume    = {12},
  journal   = {PLoS one},
  number    = {10},
  publisher = {PLoS},
  address   = {Lawrence, Kan.},
  issn      = {1932-6203},
  doi       = {10.1371/journal.pone.0186411},
  pages     = {1 -- 14},
  year      = {2017},
  abstract  = {The all-female Amazon molly (Poecilia formosa) is the result of a hybridization of the Atlantic molly (P. mexicana) and the sailfin molly (P. latipinna) approximately 120,000 years ago. As a gynogenetic species, P. formosa needs to copulate with heterospecific males including males from one of its bisexual ancestral species. However, the sperm only triggers embryogenesis of the diploid eggs. The genetic information of the sperm donor typically will not contribute to the next generation of P. formosa. Hence, P. formosa possesses generally one allele from each of its ancestral species at any genetic locus. This raises the question whether both ancestral alleles are equally expressed in P. formosa. Allele-specific expression (ASE) has been previously assessed in various organisms, e.g., human and fish, and ASE was found to be important in the context of phenotypic variability and disease. In this study, we utilized Real-Time PCR techniques to estimate ASE of the androgen receptor alpha (arα) gene in several distinct tissues of Amazon mollies. We found an allelic bias favoring the maternal ancestor (P. mexicana) allele in ovarian tissue. This allelic bias was not observed in the gill or the brain tissue. Sequencing of the promoter regions of both alleles revealed an association between an Indel in a known CpG island and differential expression. Future studies may reveal whether our observed cis-regulatory divergence is caused by an ovary-specific trans-regulatory element, preferentially activating the allele of the maternal ancestor.},
  language  = {en}
}
@article{ZhuSchluppTiedemann2016,
  author    = {Zhu, Fangjun and Schlupp, Ingo and Tiedemann, Ralph},
  title     = {Sequence Evolution and Expression of the Androgen Receptor and Other Pathway-Related Genes in a Unisexual Fish, the Amazon Molly, Poecilia formosa, and Its Bisexual Ancestors},
  series = {PLoS one},
  volume    = {11},
  journal   = {PLoS one},
  publisher = {PLoS},
  address   = {San Fransisco},
  issn      = {1932-6203},
  doi       = {10.1371/journal.pone.0156209},
  pages     = {19},
  year      = {2016},
  abstract  = {The all-female Amazon molly (Poecilia formosa) originated from a single hybridization of two bisexual ancestors, Atlantic molly (Poecilia mexicana) and sailfin molly (Poecilia latipinna). As a gynogenetic species, the Amazon molly needs to copulate with a heterospecific male, but the genetic information of the sperm-donor does not contribute to the next generation, as the sperm only acts as the trigger for the diploid eggs' embryogenesis. Here, we study the sequence evolution and gene expression of the duplicated genes coding for androgen receptors (ars) and other pathway-related genes, i.e., the estrogen receptors (ers) and cytochrome P450, family19, subfamily A, aromatase genes (cyp19as), in the Amazon molly, in comparison to its bisexual ancestors. Mollies possess-as most other teleost fish—two copies of the ar, er, and cyp19a genes, i.e., ar\&\#945;/ar\&\#946;, er\&\#945;/er\&\#946;1, and cyp19a1 (also referred as cyp19a1a)/cyp19a2 (also referred to as cyp19a1b), respectively. Non-synonymous single nucleotide polymorphisms (SNPs) among the ancestral bisexual species were generally predicted not to alter protein function. Some derived substitutions in the P. mexicana and one in P. formosa are predicted to impact protein function. We also describe the gene expression pattern of the ars and pathway-related genes in various tissues (i.e., brain, gill, and ovary) and provide SNP markers for allele specific expression research. As a general tendency, the levels of gene expression were lowest in gill and highest in ovarian tissues, while expression levels in the brain were intermediate in most cases. Expression levels in P. formosa were conserved where expression did not differ between the two bisexual ancestors. In those cases where gene expression levels significantly differed between the bisexual species, P. formosa expression was always comparable to the higher expression level among the two ancestors. Interestingly, er\&\#946;1 was expressed neither in brain nor in gill in the analyzed three molly species, which implies a more important role of er\&\#945; in the estradiol synthesis pathway in these tissues. Furthermore, our data suggest that interactions of steroid-signaling pathway genes differ across tissues, in particular the interactions of ars and cyp19as.},
  language  = {en}
}
@article{ZhuSchluppTiedemann2016,
  author    = {Zhu, Fangjun and Schlupp, Ingo and Tiedemann, Ralph},
  title     = {Sequence Evolution and Expression of the Androgen Receptor and Other Pathway-Related Genes in a Unisexual Fish, the Amazon Molly, Poecilia formosa, and Its Bisexual Ancestors},
  series = {PLoS one},
  volume    = {11},
  journal   = {PLoS one},
  number    = {6},
  publisher = {PLoS},
  address   = {Lawrence, Kan.},
  issn      = {1932-6203},
  doi       = {10.1371/JOURNAL.PONE.0156209},
  pages     = {19},
  year      = {2016},
  abstract  = {The all-female Amazon molly (Poecilia formosa) originated from a single hybridization of two bisexual ancestors, Atlantic molly (Poecilia mexicana) and sailfin molly (Poecilia latipinna). As a gynogenetic species, the Amazon molly needs to copulate with a heterospecific male, but the genetic information of the sperm-donor does not contribute to the next generation, as the sperm only acts as the trigger for the diploid eggs' embryogenesis. Here, we study the sequence evolution and gene expression of the duplicated genes coding for androgen receptors (ars) and other pathway-related genes, i.e., the estrogen receptors (ers) and cytochrome P450, family19, subfamily A, aromatase genes (cyp19as), in the Amazon molly, in comparison to its bisexual ancestors. Mollies possess-as most other teleost fish—two copies of the ar, er, and cyp19a genes, i.e., arα/arβ, erα/erβ1, and cyp19a1 (also referred as cyp19a1a)/cyp19a2 (also referred to as cyp19a1b), respectively. Non-synonymous single nucleotide polymorphisms (SNPs) among the ancestral bisexual species were generally predicted not to alter protein function. Some derived substitutions in the P. mexicana and one in P. formosa are predicted to impact protein function. We also describe the gene expression pattern of the ars and pathway-related genes in various tissues (i.e., brain, gill, and ovary) and provide SNP markers for allele specific expression research. As a general tendency, the levels of gene expression were lowest in gill and highest in ovarian tissues, while expression levels in the brain were intermediate in most cases. Expression levels in P. formosa were conserved where expression did not differ between the two bisexual ancestors. In those cases where gene expression levels significantly differed between the bisexual species, P. formosa expression was always comparable to the higher expression level among the two ancestors. Interestingly, erβ1 was expressed neither in brain nor in gill in the analyzed three molly species, which implies a more important role of erα in the estradiol synthesis pathway in these tissues. Furthermore, our data suggest that interactions of steroid-signaling pathway genes differ across tissues, in particular the interactions of ars and cyp19as.},
  language  = {en}
}
@misc{ZhuSchluppTiedemann2016,
  author    = {Zhu, Fangjun and Schlupp, Ingo and Tiedemann, Ralph},
  title     = {Sequence Evolution and Expression of the Androgen Receptor and Other Pathway-Related Genes in a Unisexual Fish, the Amazon Molly, Poecilia formosa, and Its Bisexual Ancestors},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-97119},
  pages     = {19},
  year      = {2016},
  abstract  = {The all-female Amazon molly (Poecilia formosa) originated from a single hybridization of two bisexual ancestors, Atlantic molly (Poecilia mexicana) and sailfin molly (Poecilia latipinna). As a gynogenetic species, the Amazon molly needs to copulate with a heterospecific male, but the genetic information of the sperm-donor does not contribute to the next generation, as the sperm only acts as the trigger for the diploid eggs' embryogenesis. Here, we study the sequence evolution and gene expression of the duplicated genes coding for androgen receptors (ars) and other pathway-related genes, i.e., the estrogen receptors (ers) and cytochrome P450, family19, subfamily A, aromatase genes (cyp19as), in the Amazon molly, in comparison to its bisexual ancestors. Mollies possess-as most other teleost fish—two copies of the ar, er, and cyp19a genes, i.e., arα/arβ, erα/erβ1, and cyp19a1 (also referred as cyp19a1a)/cyp19a2 (also referred to as cyp19a1b), respectively. Non-synonymous single nucleotide polymorphisms (SNPs) among the ancestral bisexual species were generally predicted not to alter protein function. Some derived substitutions in the P. mexicana and one in P. formosa are predicted to impact protein function. We also describe the gene expression pattern of the ars and pathway-related genes in various tissues (i.e., brain, gill, and ovary) and provide SNP markers for allele specific expression research. As a general tendency, the levels of gene expression were lowest in gill and highest in ovarian tissues, while expression levels in the brain were intermediate in most cases. Expression levels in P. formosa were conserved where expression did not differ between the two bisexual ancestors. In those cases where gene expression levels significantly differed between the bisexual species, P. formosa expression was always comparable to the higher expression level among the two ancestors. Interestingly, erβ1 was expressed neither in brain nor in gill in the analyzed three molly species, which implies a more important role of erα in the estradiol synthesis pathway in these tissues. Furthermore, our data suggest that interactions of steroid-signaling pathway genes differ across tissues, in particular the interactions of ars and cyp19as.},
  language  = {en}
}
@misc{ZhuSchluppTiedemann2017,
  author    = {Zhu, Fangjun and Schlupp, Ingo and Tiedemann, Ralph},
  title     = {Allele-specific expression at the androgen receptor alpha gene in a hybrid unisexual fish, the Amazon molly (Poecilia formosa)},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-403875},
  pages     = {14},
  year      = {2017},
  abstract  = {The all-female Amazon molly (Poecilia formosa) is the result of a hybridization of the Atlantic molly (P. mexicana) and the sailfin molly (P. latipinna) approximately 120,000 years ago. As a gynogenetic species, P. formosa needs to copulate with heterospecific males including males from one of its bisexual ancestral species. However, the sperm only triggers embryogenesis of the diploid eggs. The genetic information of the sperm donor typically will not contribute to the next generation of P. formosa. Hence, P. formosa possesses generally one allele from each of its ancestral species at any genetic locus. This raises the question whether both ancestral alleles are equally expressed in P. formosa. Allele-specific expression (ASE) has been previously assessed in various organisms, e.g., human and fish, and ASE was found to be important in the context of phenotypic variability and disease. In this study, we utilized Real-Time PCR techniques to estimate ASE of the androgen receptor alpha (arα) gene in several distinct tissues of Amazon mollies. We found an allelic bias favoring the maternal ancestor (P. mexicana) allele in ovarian tissue. This allelic bias was not observed in the gill or the brain tissue. Sequencing of the promoter regions of both alleles revealed an association between an Indel in a known CpG island and differential expression. Future studies may reveal whether our observed cis-regulatory divergence is caused by an ovary-specific trans-regulatory element, preferentially activating the allele of the maternal ancestor.},
  language  = {en}
}
@article{WronskiWacherHammondetal.2010,
  author    = {Wronski, Torsten and Wacher, Timothy and Hammond, Robert L. and Winney, Bruce and Hundertmark, Kris J. and Blacket, Mark J. and Mohammed, Osama B. and Flores, Benito and Omer, Sawsan A. and Macasero, William and Plath, Martin and Tiedemann, Ralph and Bleidorn, Christoph},
  title     = {Two reciprocally monophyletic mtDNA lineages elucidate the taxonomic status of Mountain gazelles (Gazella gazella)},
  issn      = {1477-2000},
  doi       = {10.1080/14772001003613192},
  year      = {2010},
  abstract  = {Mountain gazelles (Gazella gazella) rank among the most critically endangered mammals on the Arabian Peninsula. Past conservation efforts have been plagued by confusion about the phylogenetic relationship among various 'phenotypically discernable' populations, and even the question of species boundaries was far from being certain. This lack of knowledge has had a direct impact on conservation measures, especially ex situ breeding programmes, hampering the assignment of captive stocks to potential conservation units. Here, we provide a phylogenetic framework, based on the analysis of mtDNA sequences (360 bp cytochrome b and 213 bp Control Region) of 126 individuals collected from the wild throughout the Arabian Peninsula and from captive stocks. Our analyses revealed two reciprocally monophyletic genetic lineages within the presumed species Gazella gazella: one 'northern clade' on the Golan Heights (Israel/Syrian border) and one genetically diverse larger clade from the rest of the Arabian Peninsula including the Arava Valley (Negev, Israel). Applying the Strict Phylogenetic Species Concept (sensu Mishler \& Theriot, 2000) allows assigning species status to these two major clades.},
  language  = {en}
}
@article{WronskiTiedemannApioetal.2006,
  author    = {Wronski, Torsten and Tiedemann, Ralph and Apio, Ann and Plath, Martin},
  title     = {Cover, food, competitors and individual densities within bushbuck Tragelaphus scriptus female clan home ranges},
  year      = {2006},
  abstract  = {We examined factors predicting female densities within the common home ranges of related females ("clans") in bushbuck Tragelaphus scriptus Pallas, 1776. In this species, each female forms an individual home range, but the home ranges of matrilineal clan members strongly overlap. We found female densities to increase in areas with high canopy cover. Moreover, individual home range sizes tended to decrease with increasing cover. Food plant availability and intruder pressure by two heterospecific competitors did not significantly affect female densities. Apparently, canopy cover is the major limited resource in this species. A possible explanation is that both adult bushbuck and - even more markedly - fawns hide from predators in dense vegetation, in particular in thicket clumps and coalescences. The study shows an effect of habitat properties (eg sufficient canopy cover) on a within-population level in bushbuck, where female densities differ even among proximate clan areas},
  language  = {en}
}
@article{WiesnerLoxdaleKoehleretal.2011,
  author    = {Wiesner, Kerstin R. and Loxdale, Hugh D. and K{\"o}hler, G{\"u}nter and Schneider, Anja R. R. and Tiedemann, Ralph and Weisser, Wolfgang W.},
  title     = {Patterns of local and regional genetic structuring in the meadow grasshopper, Chorthippus parallelus (Orthoptera: Acrididae), in Central Germany revealed using microsatellite markers},
  series = {Biological journal of the Linnean Society : a journal of evolution},
  volume    = {103},
  journal   = {Biological journal of the Linnean Society : a journal of evolution},
  number    = {4},
  publisher = {Wiley-Blackwell},
  address   = {Malden},
  issn      = {0024-4066},
  doi       = {10.1111/j.1095-8312.2011.01698.x},
  pages     = {875 -- 890},
  year      = {2011},
  abstract  = {The meadow grasshopper, Chorthippus parallelus (Zetterstedt), is common and widespread in Central Europe, with a low dispersal range per generation. A population study in Central Germany (Frankenwald and Thuringer Schiefergebirge) showed strong interpopulation differences in abundance and individual fitness. We examined genetic variability using microsatellite markers within and between 22 populations in a short-to long-distance sampling (19 populations, Frankenwald, Schiefergebirge, as well as a southern transect), and in the Erzgebirge region (three populations), with the latter aiming to check for effects as a result of historical forest cover. Of the 671 C. parallelus captured, none was macropterous (functionally winged). All populations showed a high level of expected and observed heterozygosity (mean 0.80-0.90 and 0.60-0.75, respectively), whereas there was evidence of inbreeding (F(IS) values all positive). Allelic richness for all locus-population combinations was high (mean 9.3-11.2), whereas alleles per locus ranged from 15-62. At a local level, genic and genotypic differences were significant. Pairwise F(ST) values were in the range 0.00-0.04, indicating little interpopulation genetic differentiation. Similarly, the calculated gene flow was very high, based on the respective F(ST) (19.5) and using private alleles (7.7). A Neighbour-joining tree using Nei's D(A) and principal coordinate analysis separated two populations that were collected in the Erzgebirge region. Populations from this region may have escaped the effects of the historical forest cover. The visualization of the spatial arrangement of genotypes revealed one geographical barrier to gene flow in the short-distance sampling.},
  language  = {en}
}
@article{VernesiPecchioliTiedemannetal.2002,
  author    = {Vernesi, C. and Pecchioli, E. and Tiedemann, Ralph and Randi, E. and Bertorelle, G.},
  title     = {The genetic structure of natural and reintroduced roe deer (Capreolus capreolus) populations in the Alps and central Italy, with reference to the mitochondrial DNA phylogeography of Europe},
  issn      = {0962-1083},
  year      = {2002},
  language  = {en}
}
@article{ValenteIlleraHavensteinetal.2017,
  author    = {Valente, Luis and Illera, Juan Carlos and Havenstein, Katja and Pallien, Tamara and Etienne, Rampal S. and Tiedemann, Ralph},
  title     = {Equilibrium Bird Species Diversity in Atlantic Islands},
  series = {Current biology},
  volume    = {27},
  journal   = {Current biology},
  publisher = {Cell Press},
  address   = {Cambridge},
  issn      = {0960-9822},
  doi       = {10.1016/j.cub.2017.04.053},
  pages     = {1660 -- +},
  year      = {2017},
  language  = {en}
}
@article{TrauthMaslinDeinoetal.2010,
  author    = {Trauth, Martin H. and Maslin, Mark A. and Deino, Alan L. and Junginger, Annett and Lesoloyia, Moses and Odada, Eric O. and Olago, Daniel O. and Olaka, Lydia A. and Strecker, Manfred and Tiedemann, Ralph},
  title     = {Human evolution in a variable environment : the amplifier lakes of Eastern Africa},
  issn      = {0277-3791},
  doi       = {10.1016/j.quascirev.2010.07.007},
  year      = {2010},
  abstract  = {The development of rise Cenozoic East African Rift System (EARS) profoundly re-shaped the landscape and significantly increased the amplitude of short-term environmental response to climate variation. In particular, the development of amplifier lakes in rift basins after three million years ago significantly contributed to this exceptional sensitivity of East Africa to climate change compared to elsewhere on the African continent. Amplifier lakes are characterized by tectonically-formed graben morphologies in combination with an extreme contrast between high precipitation in the elevated parts of the catchment and high evaporation in the lake area. Such amplifier lakes respond rapidly to moderate, precessional-forced climate shifts, and as they do so apply dramatic environmental pressure to the biosphere. Rift basins, when either extremely dry or lake-filled, form important barriers for migration, mixing and competition of different populations of animals and hominins. Amplifier lakes link long-term, high-amplitude tectonic processes and short-term environmental fluctuations. East Africa may have become the place where early humans evolved as a consequence of this strong link between different time scales. (C) 2010 Elsevier Ltd. All rights reserved.},
  language  = {en}
}
@misc{TiedemannPfautsch2006,
  author    = {Tiedemann, Ralph and Pfautsch, Simone},
  title     = {MHC evolution in ducks and allies},
  series = {Journal of ornithology},
  volume    = {147},
  journal   = {Journal of ornithology},
  number    = {Supplement 1},
  publisher = {Blackwell},
  address   = {New York},
  issn      = {0021-8375},
  pages     = {48 -- 48},
  year      = {2006},
  language  = {en}
}
@article{TiedemannPaulusScheeretal.2004,
  author    = {Tiedemann, Ralph and Paulus, Kirsten B. and Scheer, M. and Von Kistowski, K. G. and Skirnisson, K. and Bloch, D. and Dam, M.},
  title     = {Mitochondrial DNA and microsatellite variation in the eider duck (Somateria mollissima) indicate stepwise postglacial colonization of Europe and limited current long-distance dispersal},
  issn      = {0962-1083},
  year      = {2004},
  abstract  = {To unravel the postglacial colonization history and the current intercolony dispersal in the common eider, Somateria mollissima, we analysed genetic variation at a part of the mitochondrial control region and five unlinked autosomal microsatellite loci in 175 eiders from 11 breeding colonies, covering the entire European distribution range of this species. As a result of extreme female philopatry, mitochondrial DNA differentiation is substantial both among local colonies and among distant geographical regions. Our study further corroborates the previous hypothesis of a single Pleistocene refugium for European eiders. A nested clade analysis on mitochondrial haplotypes suggests that (i) the Baltic Sea eider population is genetically closest to a presumably ancestral population and that (ii) the postglacial recolonization progressed in a stepwise fashion via the North Sea region and the Faroe Islands to Iceland. Current long-distance dispersal is limited. Differentiation among colonies is much less pronounced at microsatellite loci. The geographical pattern of this nuclear genetic variation is to a large extent explained by isolation by distance. As female dispersal is very limited, the geographical pattern of nuclear variation is probably explained by male-mediated gene flow among breeding colonies. Our study provides genetic evidence for the assumed prominent postglacial colonization route shaping the present terrestrial fauna of the North Atlantic islands Iceland and the Faroes. It suggests that this colonization had been a stepwise process originating in continental Europe. It is the first molecular study on eider duck populations covering their entire European distribution range},
  language  = {en}
}
@article{TiedemannPaulusHavensteinetal.2011,
  author    = {Tiedemann, Ralph and Paulus, Kirsten B. and Havenstein, Katja and Thorstensen, Sverrir and Petersen, Aevar and Lyngs, Peter and Milinkovitch, Michel C.},
  title     = {Alien eggs in duck nests brood parasitism or a help from Grandma?},
  series = {Molecular ecology},
  volume    = {20},
  journal   = {Molecular ecology},
  number    = {15},
  publisher = {Wiley-Blackwell},
  address   = {Hoboken},
  issn      = {0962-1083},
  doi       = {10.1111/j.1365-294X.2011.05158.x},
  pages     = {3237 -- 3250},
  year      = {2011},
  abstract  = {Intraspecific brood parasitism (IBP) is a remarkable phenomenon by which parasitic females can increase their reproductive output by laying eggs in conspecific females' nests in addition to incubating eggs in their own nest. Kin selection could explain the tolerance, or even the selective advantage, of IBP, but different models of IBP based on game theory yield contradicting predictions. Our analyses of seven polymorphic autosomal microsatellites in two eider duck colonies indicate that relatedness between host and parasitizing females is significantly higher than the background relatedness within the colony. This result is unlikely to be a by-product of relatives nesting in close vicinity, as nest distance and genetic identity are not correlated. For eider females that had been ring-marked during the decades prior to our study, our analyses indicate that (i) the average age of parasitized females is higher than the age of nonparasitized females, (ii) the percentage of nests with alien eggs increases with the age of nesting females, (iii) the level of IBP increases with the host females' age, and (iv) the number of own eggs in the nest of parasitized females significantly decreases with age. IBP may allow those older females unable to produce as many eggs as they can incubate to gain indirect fitness without impairing their direct fitness: genetically related females specialize in their energy allocation, with young females producing more eggs than they can incubate and entrusting these to their older relatives. Intraspecific brood parasitism in ducks may constitute cooperation among generations of closely related females.},
  language  = {en}
}
@article{TiedemannMollPaulusetal.2005,
  author    = {Tiedemann, Ralph and Moll, K. and Paulus, Kirsten B. and Schlupp, Ingo},
  title     = {New microsatellite loci confirm hybrid origin, parthenogenetic inheritance, and mitotic gene conversion in the gynogenetic Amazon molly (Poecilia formosa)},
  issn      = {1471-8278},
  year      = {2005},
  abstract  = {We describe the first microsatellite loci for the gynogenetic Amazon molly, Poecilia formosa, an all-female species arisen through hybridization of the bisexual species Poecilia mexicana and Poecilia latipinna. The loci showed one to six alleles and an expected heterozygosity between zero and 0.75. As expected with parthenogenetic inheritance, most loci were either constantly homozygous (five loci) or constantly heterozygous (eight loci). For six loci, both heterozygotes and homozygotes occurred. This and the fact that some loci only showed alleles of one of the ancestral species could indicate genome homogenization through mitotic gene conversion. Our new loci conformed to the hybrid origin of Amazon molly and are also applicable to both ancestral bisexual species},
  language  = {en}
}
@article{TebbeOttensmannHavensteinetal.2022,
  author    = {Tebbe, Jonas and Ottensmann, Meinolf and Havenstein, Katja and Efstratiou, Artemis and Lenz, Tobias L. and Caspers, Barbara A. and Forcada, Jaume and Tiedemann, Ralph and Hoffman, Joseph},
  title     = {Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals},
  series = {Scientific reports},
  volume    = {12},
  journal   = {Scientific reports},
  number    = {1},
  publisher = {Nature Publishing Group},
  address   = {London},
  issn      = {2045-2322},
  doi       = {10.1038/s41598-022-21658-7},
  pages     = {14},
  year      = {2022},
  abstract  = {The major histocompatibility complex (MHC) is a group of genes comprising one of the most important components of the vertebrate immune system. Consequently, there has been much interest in characterising MHC variation and its relationship with fitness in a variety of species. Due to the exceptional polymorphism of MHC genes, careful PCR primer design is crucial for capturing all of the allelic variation present in a given species. We therefore developed intronic primers to amplify the full-length 267 bp protein-coding sequence of the MHC class II DQB exon 2 in the Antarctic fur seal. We then characterised patterns of MHC variation among mother-offspring pairs from two breeding colonies and detected 19 alleles among 771 clone sequences from 56 individuals. The distribution of alleles within and among individuals was consistent with a single-copy, classical DQB locus showing Mendelian inheritance. Amino acid similarity at the MHC was significantly associated with genome-wide relatedness, but no relationship was found between MHC heterozygosity and genome-wide heterozygosity. Finally, allelic diversity was several times higher than reported by a previous study based on partial exon sequences. This difference appears to be related to allele-specific amplification bias, implying that primer design can strongly impact the inference of MHC diversity.},
  language  = {en}
}
@article{TaguchiGotoMatsuokaetal.2023,
  author    = {Taguchi, Mioko and Goto, Mutsuo and Matsuoka, Koji and Tiedemann, Ralph and Pastene, Luis A.},
  title     = {Population genetic structure of Bryde's whales (Balaenoptera brydei) on the central and western North Pacific feeding grounds},
  series = {Canadian Journal of Fisheries and Aquatic Sciences},
  volume    = {80},
  journal   = {Canadian Journal of Fisheries and Aquatic Sciences},
  number    = {1},
  publisher = {Canadian science publishing},
  address   = {Ottawa},
  issn      = {0706-652X},
  doi       = {10.1139/cjfas-2022-0005},
  pages     = {142 -- 155},
  year      = {2023},
  abstract  = {The genetic structure of Bryde's whale (Balaenoptera brydei) on the central and western North Pacific feeding grounds was investigated using a total of 1195 mitochondrial control region sequences and 1182 microsatellite genotypes at 17 loci in specimens collected from three longitudinal areas, 1W (135 degrees E-165 degrees E), 1E (165 degrees E-180 degrees), and 2 (180 degrees-155 degrees W). Genetic diversities were similar among areas and a haplotype network did not show any geographic structure, while an analysis of molecular variance found evidence of genetic structure in this species. Pairwise FST and G'ST estimates and heterogeneity tests attributed this structure to weak but significant differentiation between areas 1W/1E and 2. A Mantel test and a high-resolution analysis of genetic diversity statistics showed a weak spatial cline of genetic differentiation. These findings could be reconciled by two possible stock structure scenarios: (1) a single population with kin-association affecting feeding ground preference and (2) two populations with feeding ground preference for either area 1W or area 2. An estimated dispersal rate between areas 1W and 2 indicates that both scenarios should be considered as a precautionary principle in stock assessments.},
  language  = {en}
}
@article{StuckasStoofQuesadaetal.2009,
  author    = {Stuckas, Heiko and Stoof, Kathleen and Quesada, Humberto and Tiedemann, Ralph},
  title     = {Evolutionary implications of discordant clines across the Baltic Mytilus hybrid zone (Mytilus edulis and Mytilus trossulus)},
  issn      = {0018-067X},
  doi       = {10.1038/Hdy.2009.37},
  year      = {2009},
  abstract  = {It is well established that reproductive isolation often arises from genome incompatibilities and that genes encoding reproductive traits are less prone to introgression. Hybrid zones of Mytilus trossulus and Mytilus edulis provide an intriguing model to assess reproductive isolation. Although gene flow is restricted in North America, introgression is pervasive in the Baltic. This study aimed at analyzing the shape of multilocus clines across the Baltic contact zone between M. edulis and M. trossulus to infer mechanisms of restriction to gene flow. We use maximum likelihood methods to construct the best fitting individual clines for five markers located on biparentally inherited autosomes and paternally and maternally inherited mitochondrial DNA (mtDNA). Strong cline shape differences among markers suggest that reproductive isolation arising from genome-wide incompatibilities is weak, and that these discrepancies possibly result from genetic drift, hybrid zone movement or marker-specific selection. However, the finding of a common cline center for M7 lysin (involved in fertilization) and paternally transmitted mtDNA (causing nuclear-mitochondrial incompatibilities in hybrids) suggest that these loci may play a role in incomplete reproductive isolation.},
  language  = {en}
}