@article{PecherHarnackGuntheretal.2001,
  author    = {Pecher, Gabriele and Harnack, U. and Gunther, M. and Hummel, M. and Fichtner, I. and Schenk, J{\"o}rg A.},
  title     = {Generation of an immortalized human CD4+ T cell clone inhibiting tumor growth in mice.},
  year      = {2001},
  abstract  = {Tumor antigen-specific T cell clones represent a useful tool in tumor immunology; however, their long-term culture is limited. To generate an immortalized cytotoxic T cell clone against the human tumor antigen mucin, we exposed a previously generated T cell culture to Herpesvirus saimiri. We obtained an immortalized human CD4+ T cell clone, termed SITAM. Clonality of these cells was shown by analysis of the alpha/beta-T cell receptor (TCR) repertoire. Cytolytic activity was demonstrated against several mucin-expressing tumor cell lines and could not be detected against non-mucin-expressing cells. SITAM cells maintained their features stably for 2 years. Furthermore, growth of the tumor cell line Capan-2 in NOD/SCID mice was inhibited when SITAM cells were coinjected subcutaneously with tumor cells. SITAM cells provide an unlimited source of clonal T cells for analysis of tumor recognition and may be of help in TCR-targeted immunotherapy.},
  language  = {en}
}
@article{ClermontWeddeSeitzetal.2004,
  author    = {Clermont, A. and Wedde, M. and Seitz, V. and Podsiadlowski, L. and Lenze, D. and Hummel, M. and Vilcinskas, Andreas},
  title     = {Cloning and expression of an inhibitor of microbial metalloproteinases from insects contributing to innate immunity},
  issn      = {0264-6021},
  year      = {2004},
  abstract  = {The first IMPI (inhibitor of metalloproteinases from insects) was identified in the greater wax moth, Galleria mellonella [Wedde, Weise, Kopacek, Franke and Vilcinskas (1998) Eur. J. Biochem. 255, 535-543]. Here we report cloning and expression of a cDNA coding for this IMPI. The IMPI mRNA was identified among the induced transcripts from a subtractive and suppressive PCR analysis after bacterial challenge of G. mellonella larvae. Induced expression of the IMPI during a Immoral immune response was confirmed by real-time PCR, which documented up to 500 times higher amounts of IMPI mRNA in immunized larvae in comparison with untreated ones. The IMPI sequence shares no similarity with those of tissue inhibitors of metalloproteinases or other natural inhibitors of metalloproteinases, and the recombinant IMPI specifically inhibits thermolysin-like metalloproteinases, but not matrix metalloproteinases. These results support the hypothesis that the IMPI represents a novel type of immune-related protein which is induced and processed during the G. mellonella humoral immune response to inactivate pathogen-associated thermolysin-like metalloproteinases},
  language  = {en}
}