@article{TadaGrossart2014,
  author    = {Tada, Yuya and Grossart, Hans-Peter},
  title     = {Community shifts of actively growing lake bacteria after N-acetyl-glucosamine addition: improving the BrdU-FACS method},
  series = {The ISME journal : multidisciplinary journal of microbial ecology},
  volume    = {8},
  journal   = {The ISME journal : multidisciplinary journal of microbial ecology},
  number    = {2},
  publisher = {Nature Publ. Group},
  address   = {London},
  issn      = {1751-7362},
  doi       = {10.1038/ismej.2013.148},
  pages     = {441 -- 454},
  year      = {2014},
  abstract  = {In aquatic environments, community dynamics of bacteria, especially actively growing bacteria (AGB), are tightly linked with dissolved organic matter (DOM) quantity and quality. We analyzed the community dynamics of DNA-synthesizing and accordingly AGB by linking an improved bromodeoxyuridine immunocytochemistry approach with fluorescence-activated cell sorting (BrdU-FACS). FACS-sorted cells of even oligotrophic ecosystems in winter were characterized by 16S rRNA gene analysis. In incubation experiments, we examined community shifts of AGB in response to the addition of N-acetyl-glucosamine (NAG), one of the most abundant aminosugars in aquatic systems. Our improved BrdU-FACS analysis revealed that AGB winter communities of oligotrophic Lake Stechlin (northeastern Germany) substantially differ from those of total bacteria and consist of Alpha-, Beta-, Gamma-, Deltaproteobacteria, Actinobacteria, Candidatus OP10 and Chloroflexi. AGB populations with different BrdU-fluorescence intensities and cell sizes represented different phylotypes suggesting that single-cell growth potential varies at the taxon level. NAG incubation experiments demonstrated that a variety of widespread taxa related to Alpha-, Beta-, Gammaproteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, Planctomycetes, Spirochaetes, Verrucomicrobia and Chloroflexi actively grow in the presence of NAG. The BrdU-FACS approach enables detailed phylogenetic studies of AGB and, thus, to identify those phylotypes which are potential key players in aquatic DOM cycling.},
  language  = {en}
}