@article{DaiMateGlebeetal.2018,
  author    = {Dai, Xiaolin and Mate, Diana M. and Glebe, Ulrich and Garakani, Tayebeh Mirzaei and K{\"o}rner, Andrea and Schwaneberg, Ulrich and B{\"o}ker, Alexander},
  title     = {Sortase-mediated ligation of purely artificial building blocks},
  series = {Polymers},
  volume    = {10},
  journal   = {Polymers},
  number    = {2},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2073-4360},
  doi       = {10.3390/polym10020151},
  pages     = {13},
  year      = {2018},
  abstract  = {Sortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP-polymer, NP-NP, and polymer-polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction-the conserved peptide LPETG-and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated.},
  language  = {en}
}