@misc{Beta2010, author = {Beta, Carsten}, title = {Bistability in the actin cortex}, series = {Postprints der Universit{\"a}t Potsdam Mathematisch-Naturwissenschaftliche Reihe}, volume = {3}, journal = {Postprints der Universit{\"a}t Potsdam Mathematisch-Naturwissenschaftliche Reihe}, number = {12}, doi = {10.25932/publishup-42938}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-429385}, pages = {9}, year = {2010}, abstract = {Multi-color fluorescence imaging experiments of wave forming Dictyostelium cells have revealed that actin waves separate two domains of the cell cortex that differ in their actin structure and phosphoinositide composition. We propose a bistable model of actin dynamics to account for these experimental observation. The model is based on the simplifying assumption that the actin cytoskeleton is composed of two distinct network types, a dendritic and a bundled network. The two structurally different states that were observed in experiments correspond to the stable fixed points in the bistable regime of this model. Each fixed point is dominated by one of the two network types. The experimentally observed actin waves can be considered as trigger waves that propagate transitions between the two stable fixed points.}, language = {en} } @misc{BreitensteinHoelzelBier2010, author = {Breitenstein, Michael and H{\"o}lzel, Ralph and Bier, Frank Fabian}, title = {Immobilization of different biomolecules by atomic force microscopy}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {872}, issn = {1866-8372}, doi = {10.25932/publishup-43507}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-435075}, pages = {9}, year = {2010}, abstract = {Background Micrometer resolution placement and immobilization of probe molecules is an important step in the preparation of biochips and a wide range of lab-on-chip systems. Most known methods for such a deposition of several different substances are costly and only suitable for a limited number of probes. In this article we present a flexible procedure for simultaneous spatially controlled immobilization of functional biomolecules by molecular ink lithography. Results For the bottom-up fabrication of surface bound nanostructures a universal method is presented that allows the immobilization of different types of biomolecules with micrometer resolution. A supporting surface is biotinylated and streptavidin molecules are deposited with an AFM (atomic force microscope) tip at distinct positions. Subsequent incubation with a biotinylated molecule species leads to binding only at these positions. After washing streptavidin is deposited a second time with the same AFM tip and then a second biotinylated molecule species is coupled by incubation. This procedure can be repeated several times. Here we show how to immobilize different types of biomolecules in an arbitrary arrangement whereas most common methods can deposit only one type of molecules. The presented method works on transparent as well as on opaque substrates. The spatial resolution is better than 400 nm and is limited only by the AFM's positional accuracy after repeated z-cycles since all steps are performed in situ without moving the supporting surface. The principle is demonstrated by hybridization to different immobilized DNA oligomers and was validated by fluorescence microscopy. Conclusions The immobilization of different types of biomolecules in high-density microarrays is a challenging task for biotechnology. The method presented here not only allows for the deposition of DNA at submicrometer resolution but also for proteins and other molecules of biological relevance that can be coupled to biotin.}, language = {en} } @misc{Budke2010, author = {Budke, Alexandra}, title = {Und der Zukunft abgewandt}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {813}, isbn = {978-3-89971-627-6}, issn = {1866-8372}, doi = {10.25932/publishup-41080}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-410801}, pages = {411}, year = {2010}, abstract = {Seit dem Ende der DDR, das den Zusammenbruch des Ostblocks und damit die Beendigung des »Kalten Kriegs« einleitete, wird verst{\"a}rkt versucht, das Wesen dieses Staates zu definieren und damit seine Folgen auf wirtschaftlicher, sozialer, psychologischer und bildungspolitischer Ebene zu verstehen und einzuordnen. Alexandra Budke analysiert in diesem Band das Schulfach Geographie, das neben der Staatsb{\"u}rgerkunde und der Geschichte ein zentrales Fach war und in dem die in den Lehrpl{\"a}nen definierte »staatsb{\"u}rgerliche, weltanschauliche oder ideologische Erziehung« auf der Grundlage des Marxismus-Leninismus stattfinden sollte. Sie kl{\"a}rt, inwiefern Geographieunterricht in der DDR genutzt wurde, um geopolitische Interessen des Staates zu kommunizieren und zu verbreiten. Damit l{\"a}sst sich durch die detaillierte Analyse des Fachunterrichts auch die Frage beantworten, ob Sch{\"u}lerInnen im Unterricht politisch manipuliert wurden und welche Handlungsm{\"o}glichkeiten die zentralen Akteure des Unterrichts, die LehrerInnen und die Sch{\"u}lerInnen, im Rahmen der durch die Bildungspolitik gesetzten curricularen Vorgaben wahrgenommen haben.}, language = {de} } @misc{CarlsohnCasselLinneetal.2010, author = {Carlsohn, Anja and Cassel, Michael and Linn{\´e}, Karsten and Mayer, Frank}, title = {How much is too much?}, series = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, number = {515}, issn = {1866-8364}, doi = {10.25932/publishup-41291}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-412910}, pages = {5}, year = {2010}, abstract = {Although dietary nutrient intake is often adequate, nutritional supplement use is common among elite athletes. However, high-dose supplements or the use of multiple supplements may exceed the recommended daily allowance (RDA) of particular nutrients or even result in a daily intake above tolerable upper limits (UL). The present case report presents nutritional intake data and supplement use of a highly trained male swimmer competing at international level. Habitual energy and micronutrient intake were analysed by 3 d dietary reports. Supplement use and dosage were assessed, and total amount of nutrient supply was calculated. Micronutrient intake was evaluated based on RDA and UL as presented by the European Scientific Committee on Food, and maximum permitted levels in supplements (MPL) are given. The athlete's diet provided adequate micronutrient content well above RDA except for vitamin D. Simultaneous use of ten different supplements was reported, resulting in excess intake above tolerable UL for folate, vitamin E and Zn. Additionally, daily supplement dosage was considerably above MPL for nine micronutrients consumed as artificial products. Risks and possible side effects of exceeding UL by the athlete are discussed. Athletes with high energy intake may be at risk of exceeding UL of particular nutrients if multiple supplements are added. Therefore, dietary counselling of athletes should include assessment of habitual diet and nutritional supplement intake. Educating athletes to balance their diets instead of taking supplements might be prudent to prevent health risks that may occur with long-term excess nutrient intake.}, language = {en} } @misc{Crome2010, author = {Crome, Erhard}, title = {Es geht nicht um "Versagen"!}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-44386}, year = {2010}, abstract = {Die sich zun{\"a}chst um Griechenland rankende Krise wurde zu einer europ{\"a}ischen, die den Euro bedroht. Allenthalben wird die Frage gestellt: Wer ist schuld? In deutschen Groß-Medien wird auf das Z{\"o}gern der derzeitigen Bundesregierung verwiesen. Der Euro rutschte in die Gefahrenzone. Aber ist der Verweis auf falsche Politik die Antwort auf die Frage nach dem erneuten Aufbrechen der Finanzkrise?}, language = {de} } @misc{DortayMuellerRoeber2010, author = {Dortay, Hakan and M{\"u}ller-R{\"o}ber, Bernd}, title = {A highly efficient pipeline for protein expression in Leishmania tarentolae sing infrared fluorescence protein as marker}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-44773}, year = {2010}, abstract = {Background: Leishmania tarentolae, a unicellular eukaryotic protozoan, has been established as a novel host for recombinant protein production in recent years. Current protocols for protein expression in Leishmania are, however, time consuming and require extensive lab work in order to identify well-expressing cell lines. Here we established an alternative protein expression work-flow that employs recently engineered infrared fluorescence protein (IFP) as a suitable and easy-to-handle reporter protein for recombinant protein expression in Leishmania. As model proteins we tested three proteins from the plant Arabidopsis thaliana, including a NAC and a type-B ARR transcription factor. Results: IFP and IFP fusion proteins were expressed in Leishmania and rapidly detected in cells by deconvolution microscopy and in culture by infrared imaging of 96-well microtiter plates using small cell culture volumes (2 μL - 100 μL). Motility, shape and growth of Leishmania cells were not impaired by intracellular accumulation of IFP. In-cell detection of IFP and IFP fusion proteins was straightforward already at the beginning of the expression pipeline and thus allowed early pre-selection of well-expressing Leishmania clones. Furthermore, IFP fusion proteins retained infrared fluorescence after electrophoresis in denaturing SDS-polyacrylamide gels, allowing direct in-gel detection without the need to disassemble cast protein gels. Thus, parameters for scaling up protein production and streamlining purification routes can be easily optimized when employing IFP as reporter. Conclusions: Using IFP as biosensor we devised a protocol for rapid and convenient protein expression in Leishmania tarentolae. Our expression pipeline is superior to previously established methods in that it significantly reduces the hands-on-time and work load required for identifying well-expressing clones, refining protein production parameters and establishing purification protocols. The facile in-cell and in-gel detection tools built on IFP make Leishmania amenable for high-throughput expression of proteins from plant and animal sources.}, language = {en} } @misc{Eckstein2010, author = {Eckstein, Lars}, title = {M.I.A.'s "Born Free" and the ambivalent politics of authenticity and provocation}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-59236}, year = {2010}, language = {en} } @misc{Eggert2010, author = {Eggert, Sandy}, title = {ERP-Architekturen}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-44481}, year = {2010}, language = {de} } @misc{EggertMeier2010, author = {Eggert, Sandy and Meier, Juliane}, title = {Business Intelligence : L{\"o}sungen im {\"U}berblick}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-44493}, year = {2010}, language = {de} } @misc{FanselowLenertova2010, author = {Fanselow, Gisbert and Lenertov{\´a}, Denisa}, title = {Left peripheral focus}, series = {Humanwissenschaftliche Reihe}, journal = {Humanwissenschaftliche Reihe}, number = {596}, issn = {1866-8364}, doi = {10.25932/publishup-42819}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-428198}, pages = {43}, year = {2010}, abstract = {In Czech, German, and many other languages, part of the semantic focus of the utterance can be moved to the left periphery of the clause. The main generalization is that only the leftmost accented part of the semantic focus can be moved. We propose that movement to the left periphery is generally triggered by an unspecific edge feature of C (Chomsky 2008) and its restrictions can be attributed to requirements of cyclic linearization, modifying the theory of cyclic linearization developed by Fox and Pesetsky (2005). The crucial assumption is that structural accent is a direct consequence of being linearized at merge, thus it is indirectly relevant for (locality restrictions on) movement. The absence of structural accent correlates with given-ness. Given elements may later receive (topic or contrastive) accents, which accounts for fronting in multiple focus/contrastive topic constructions. Without any additional assumptions, the model can account for movement of pragmatically unmarked elements to the left periphery ('formal fronting', Frey 2005). Crucially, the analysis makes no reference at all to concepts of information structure in the syntax, in line with the claim of Chomsky (2008) that UG specifies no direct link between syntax and information structure.}, language = {en} } @misc{Fischer2010, author = {Fischer, Karl}, title = {Verhandlungen oder milit{\"a}rische Option? : Chancen und Risiken f{\"u}r Afghanistan}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-48984}, year = {2010}, abstract = {Nach wie vor ist es der internationalen Gemeinschaft nicht gelungen, eine L{\"o}sung f{\"u}r die afghanische Krise zu pr{\"a}sentieren. Dabei macht die gegenw{\"a}rtige Situation eine Beendigung des Kriegszustands sowie die Aufnahme von konstruktiven Verhandlungen unerl{\"a}sslich. Die Genfer Verhandlungen der 1980er Jahre {\"u}ber den Abzug der Sowjetarmee aus Afghanistan k{\"o}nnten hierbei als Vorbild dienen.}, language = {de} } @misc{Franzke2010, author = {Franzke, Jochen}, title = {Chefdiplomat versus Missionarin}, isbn = {978-3-941880-06-1}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-40824}, year = {2010}, abstract = {Die Frage, ob die CDU-Bundestagsabgeordnete Erika Steinbach, seit 1998 Pr{\"a}sidenten des Bundes der Vertriebenen (BdV), einen Sitz im Stiftungsrat des "Zentrums gegen Vertreibung" einnehmen soll, spaltet die politische Landschaft. Die von der SPD beantragte Aktuelle Stunde des Bundestages am 26. November 2009 machte dies erneut deutlich. Auch die neue Bundesregierung ist in dieser Frage gespalten.}, language = {de} } @misc{Giessmann2010, author = {Gießmann, Hans Joachim}, title = {Jenseits unserer Wahrnehmung : die vergessenen Konflikte}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-46432}, year = {2010}, abstract = {Gegenw{\"a}rtig toben an die 40 Kriege und bewaffnete Konflikte in der Welt. Die meisten dieser Krisenherde spielen in den Medien eine untergeordnete Rolle und sind der {\"o}ffentlichen Wahrnehmung entr{\"u}ckt: Sie werden vergessen. Der Autor diskutiert die Gr{\"u}nde f{\"u}r diese Entr{\"u}ckung: klar und kritisch, pointiert und provozierend.}, language = {de} } @misc{HajialioghliMoazzenJahangirietal.2010, author = {Hajialioghli, Robab and Moazzen, Mohssen and Jahangiri, Ahmad and Oberh{\"a}nsli, Roland and Mocek, Beate and Altenberger, Uwe}, title = {Petrogenesis and tectonic evolution of metaluminous sub-alkaline granitoids from the Takab Complex, NW Iran}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {570}, issn = {1866-8372}, doi = {10.25932/publishup-41310}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-413100}, pages = {19}, year = {2010}, abstract = {The Takab complex is composed of a variety of metamorphic rocks including amphibolites, metapelites, mafic granulites, migmatites and meta-ultramafics, which are intruded by the granitoid. The granitoid magmatic activity occurred in relation to the subduction of the Neo-Tethys oceanic crust beneath the Iranian crust during Tertiary times. The granitoids are mainly granodiorite, quartz monzodiorite, monzonite and quartz diorite. Chemically, the magmatic rocks are characterized by ASI < 1.04, AI < 0.87 and high contents of CaO (up to ∼ 14.5 wt \%), which are consistent with the I-type magmatic series. Low FeO t /(FeO t +MgO) values (< 0.75) as well as low Nb, Y and K 2 O contents of the investigated rocks resemble the calc-alkaline series. Low SiO 2 , K 2 O/Na 2 O and Al 2 O 3 accompanied by high CaO and FeO contents indicate melting of metabasites as an appropriate source for the intrusions. Negative Ti and Nb anomalies verify a metaluminous crustal origin for the protoliths of the investigated igneous rocks. These are comparable with compositions of the associated mafic migmatites, in the Takab metamorphic complex, which originated from the partial melting of amphibolites. Therefore, crustal melting and a collision-related origin for the Takab calc-alkaline intrusions are proposed here on the basis of mineralogy and geochemical characteristics. The P-T evolution during magmatic crystallization and subsolidus cooling stages is determined by the study of mineral chemistry of the granodiorite and the quartz diorite. Magmatic crystallization pressure and temperature for the quartz-diorite and the granodiorite are estimated to be P ∼ 7.8 ± 2.5 kbar, T ∼ 760 ± 75 ◦C and P ∼ 5 ± 1 kbar, T ∼ 700 ◦C, respectively. Subsolidus conditions are consistent with temperatures of ∼ 620 ◦C and ∼ 600 ◦C, and pressures of ∼ 5 kbar and ∼ 3.5 kbar for the quartz-diorite and the granodiorite, respectively.}, language = {en} } @misc{HohensteinLaubrockKliegl2010, author = {Hohenstein, Sven and Laubrock, Jochen and Kliegl, Reinhold}, title = {Semantic preview benefit in eye movements during reading: a parafoveal past-priming study}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-57203}, year = {2010}, abstract = {Eye movements in reading are sensitive to foveal and parafoveal word features. Whereas the influence of orthographic or phonological parafoveal information on gaze control is undisputed, there has been no reliable evidence for early parafoveal extraction of semantic information in alphabetic script. Using a novel combination of the gaze-contingent fast-priming and boundary paradigms, we demonstrate semantic preview benefit when a semantically related parafoveal word was available during the initial 125 ms of a fixation on the pre-target word (Experiments 1 and 2). When the target location was made more salient, significant parafoveal semantic priming occurred only at 80 ms (Experiment 3). Finally, with short primes only (20, 40, 60 ms) effects were not significant but numerically in the expected direction for 40 and 60 ms (Experiment 4). In all experiments, fixation durations on the target word increased with prime durations under all conditions. The evidence for extraction of semantic information from the parafoveal word favors an explanation in terms of parallel word processing in reading.}, language = {en} } @misc{Hopf2010, author = {Hopf, Diether}, title = {Erfolgreiches Lehren in der Schule : Anmerkungen zum Stand der Forschung}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-53814}, year = {2010}, abstract = {Der Aufsatz befasst sich mit der Frage, was man aus der internationalen Forschung dar{\"u}ber weiß, welcher Unterricht und welches Lehrerverhalten Auswirkungen auf die Lernergebnisse der Sch{\"u}ler/innen hat und welche M{\"o}glichkeiten es gibt, solches Verhalten zu verst{\"a}rken. Ber{\"u}cksichtigt werden nur Forschungsergebnisse, die hohen methodischen Anforderungen gen{\"u}gen, kausale Schlussfolgerungen erlauben und generalisierbar sind. Konsequenzen f{\"u}r die Lehrerbildung werden diskutiert.}, language = {de} } @misc{Hopf2010, author = {Hopf, Diether}, title = {Anreize zu erfolgreichem Lehren in der Schule : Forschungsstand und methodologische {\"U}berlegungen ; Langfassung}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-53829}, year = {2010}, abstract = {Der Aufsatz befasst sich mit der Frage, was man aus der internationalen Forschung dar{\"u}ber weiß, welcher Unterricht und welches Lehrerverhalten Auswirkungen auf die Lernergebnisse der Sch{\"u}ler/innen hat und welche M{\"o}glichkeiten es gibt, solches Verhalten zu verst{\"a}rken. Ber{\"u}cksichtigt werden nur Forschungsergebnisse, die hohen methodischen Anforderungen gen{\"u}gen, kausale Schlussfolgerungen erlauben und generalisierbar sind. Konsequenzen f{\"u}r die Lehrerbildung werden diskutiert.}, language = {de} } @misc{ImhofRudolph2010, author = {Imhof-Rudolph, Heike}, title = {Wehrpflicht ade - tut Scheiden weh?}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-48970}, year = {2010}, abstract = {Wieviel Bundeswehr braucht Deutschland? Und wozu? Das prim{\"a}re Ziel der Bundeswehr, die Landesverteidigung, ist ein genauso antiquierter Begriff wie ihre Struktur. Welche Transformation der Streitkr{\"a}fte ist geboten?}, language = {de} } @misc{Kiraly2010, author = {Kiraly, Attila}, title = {Menetekel}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-42295}, year = {2010}, abstract = {Die Verfasstheit der Europ{\"a}ischen Union n{\"a}hert sich der Staatlichkeit an: Der Euro ist Ausdruck dessen, dass die W{\"a}hrungshoheit der beteiligten Staaten in Br{\"u}ssel abgegeben ist; die Milit{\"a}reins{\"a}tze der EU, darunter in Kosovo, Kongo, Tschad und vor der somalischen K{\"u}ste, zeugen vom Ausbau einer eigenst{\"a}ndigen Kriegf{\"u}hrungsf{\"a}higkeit, die im Vertrag von Lissabon auch v{\"o}lkerrechtlich fixiert ist; die Ernennung von Lady Ashton zur EU-Außenbeauftragten und die Schaffung eines eigenen außenpolitischen Dienstes weisen darauf hin, dass auch das Recht der Außenvertretung schrittweise auf die Unionsebene {\"u}bergeht.}, language = {de} } @misc{Kleinwaechter2010, author = {Kleinw{\"a}chter, Lutz}, title = {Reif f{\"u}r die Weltpolitik?}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-41627}, year = {2010}, abstract = {Bereits vor zehn Jahren diskutierten wir {\"u}ber die außenpolitische Reife deutscher Eliten. Das Ergebnis war widerspr{\"u}chlich, kritisch ern{\"u}chternd. Gegenw{\"a}rtig, 20 Jahre nach der deutschen Einheit, ist es an der Zeit, erneut nachzufragen. Die Welt- und Europapolitik sind im Fieber. Die neokonservative Marktwirtschaft - bis vor Kurzem noch siegessicher - erodiert. Die westliche Welt steckt am Beginn des 21. Jahrhunderts in einer Systemkrise. Hektische Gipfel jagen einander, von Kopenhagen {\"u}ber London und Davos bis nach M{\"u}nchen. Da ist die Frage erlaubt: Wie steht es in solch turbulenten Zeiten eigentlich um die deutsche Außenpolitik?}, language = {de} }