@article{HilleBergBresseletal.2008,
  author    = {Hille, Carsten and Berg, Maik and Bressel, Lena and Munzke, Dorit and Primus, Philipp and L{\"o}hmannsr{\"o}ben, Hans-Gerd and Dosche, Carsten},
  title     = {Time-domain fluorescence lifetime imaging for intracellular pH sensing in living tissues},
  doi       = {10.1007/s00216-008-2147-0},
  year      = {2008},
  abstract  = {pH sensing in living cells represents one of the most prominent topics in biochemistry and physiology. In this study we performed one-photon and two-photon time-domain fluorescence lifetime imaging with a laser-scanning microscope using the time-correlated single-photon counting technique for imaging intracellular pH levels. The suitability of different commercial fluorescence dyes for lifetime-based pH sensing is discussed on the basis of in vitro as well of in situ measurements. Although the tested dyes are suitable for intensity-based ratiometric measurements, for lifetime- based techniques in the time-domain so far only BCECF seems to meet the requirements of reliable intracellular pH recordings in living cells.},
  language  = {en}
}