@article{SteffenvonNickischRosenegkBier2005,
  author    = {Steffen, Jenny and von Nickisch-Rosenegk, Markus and Bier, Frank Fabian},
  title     = {In vitro transcription of a whole gene on a surface-coupled template},
  issn      = {1473-0189},
  year      = {2005},
  abstract  = {An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated},
  language  = {en}
}