@article{MichardLacombePoreeetal.2005,
  author    = {Michard, Erwan and Lacombe, Beno{\^i}t and Poree, Fabien and M{\"u}ller-R{\"o}ber, Bernd and Sentenac, Herv{\´e} and Thibaud, Jean-Baptiste and Dreyer, Ingo},
  title     = {A unique voltage sensor sensitizes the potassium channel AKT2 to phosphoregulation},
  year      = {2005},
  abstract  = {Among all voltage-gated K+ channels from the model plant Arabidopsis thaliana, the weakly rectifying K+ channel (K-weak channel) AKT2 displays unique gating properties. AKT2 is exceptionally regulated by phosphorylation: when nonphosphorylated AKT2 behaves as an inward-rectifying potassium channel; phosphorylation of AKT2 abolishes inward rectification by shifting its activation threshold far positive (>200 mV) so that it closes only at voltages positive of + 100 mV. In its phosphorylated form, AKT2 is thus locked in the open state in the entire physiological voltage range. To understand the molecular grounds of this unique gating behavior, we generated chimeras between AKT2 and the conventional inward-rectifying channel KAT1. The transfer of the pore from KAT1 to AKT2 altered the permeation properties of the channel. However, the gating properties were unaffected, suggesting that the pore region of AKT2 is not responsible for the unique K-weak gating. Instead, a lysine residue in S4, highly conserved among all K-weak channels but absent from other plant K+ channels, was pinpointed in a site-directed mutagenesis approach. Substitution of the lysine by serine or aspartate abolished the "open-lock" characteristic and converted AKT2 into an inward- rectifying channel. Interestingly, phosphoregulation of the mutant AKT2-K197S appeared to be similar to that of the K-in channel KAT1: as suggested by mimicking the phosphorylated and dephosphorylated states, phosphorylation induced a shift of the activation threshold of AKT2-K197S by about +50 mV. We conclude that the lysine residue K197 sensitizes AKT2 to phosphoregulation. The phosphorylation-induced reduction of the activation energy in AKT2 is similar to 6 kT larger than in the K197S mutant. It is discussed that this hypersensitive response of AKT2 to phosphorylation equips a cell with the versatility to establish a potassium gradient and to make efficient use of it},
  language  = {en}
}
@article{RautengartenSteinhaeuserBussisetal.2005,
  author    = {Rautengarten, Carsten and Steinhaeuser, Dirk and Bussis, D and Stintzi, A and Schaller, A and Kopka, Joachim and Altmann, Thomas},
  title     = {Inferring hypotheses on functional relationships of genes : Analysis of the Arabidopsis thaliana subtilase gene family},
  year      = {2005},
  abstract  = {The gene family of subtilisin-like serine proteases (subtilases) in Arabidopsis thaliana comprises 56 members, divided into six distinct subfamilies. Whereas the members of five subfamilies are similar to pyrolysins, two genes share stronger similarity to animal kexins. Mutant screens confirmed 144 T-DNA insertion lines with knockouts for 55 out of the 56 subtilases. Apart from SDD1, none of the confirmed homozygous mutants revealed any obvious visible phenotypic alteration during growth under standard conditions. Apart from this specific case, forward genetics gave us no hints about the function of the individual 54 non-characterized subtilase genes. Therefore, the main objective of our work was to overcome the shortcomings of the forward genetic approach and to infer alternative experimental approaches by using an integrative biolinformatics and biological approach. Computational analyses based on transcriptional co-expression and co-response pattern revealed at least two expression networks, suggesting that functional redundancy may exist among subtilases with limited similarity. Furthermore, two hubs were identified, which may be involved in signalling or may represent higher-order regulatory factors involved in responses to environmental cues. A particular enrichment of co- regulated genes with metabolic functions was observed for four subtilases possibly representing late responsive elements of environmental stress. The kexin homologs show stronger associations with genes of transcriptional regulation context. Based on the analyses presented here and in accordance with previously characterized subtilases, we propose three main functions of subtilases: involvement in (i) control of development, (ii) protein turnover, and (iii) action as downstream components of signalling cascades},
  language  = {en}
}
@article{BolayBraunDelheyetal.2005,
  author    = {Bolay, Adrien and Braun, Uwe and Delhey, Rolf and Kummer, Volker and Piatek, Marcin and Wolczanska, Agata},
  title     = {Erysiphe deutziae - a new epidemic spread in Europe},
  year      = {2005},
  abstract  = {Erysiphe deutziae (Bunkina) U. Braun \& S. Takam. is powdery mildew fungus that is currently spreading in Europe. The anamorph of this species has been found in France, Germany, Poland and Switzerland on Deutzia sp. (cult.), Deutzia x magnifica (Lemoine) Rehder and Deutzia scabra Thunb. The morphology, taxonomy and worldwide distribution of Erysiphe deutziae are described, illustrated and discussed},
  language  = {en}
}
@article{MaurerGautschiWeyandetal.2005,
  author    = {Maurer, Katrin and Gautschi, B and Weyand, Anne and Stocklin, J and Fischer, Markus},
  title     = {Isolation and characterization of microsatellite DNA markers in the grass Poa alpina L.},
  year      = {2005},
  abstract  = {The important fodder grass Poa alpina L. occurs at several ploidy levels with common aneuploidy. We isolated and characterized five polymorphic microsatellite markers for the study of molecular genetic variation of this species. As first examples of the value of the developed markers for population genetic analyses, we show that plants with more chromosomes have more microsatellite bands and that isolation by distance plays a small role in shaping microsatellite diversity of P. alpina in the Swiss Alps},
  language  = {en}
}
@article{Wacker2005,
  author    = {Wacker, Alexander},
  title     = {Lipids in the food of a terrestrial snail},
  year      = {2005},
  abstract  = {Animals depend on a large set of essential compounds in their food. A reduced supply of specific lipid compounds has been shown to be critical for growth, reproduction and survival of aquatic molluscs and might also be critical for terrestrial gastropods. The effects were investigated of different fatty acid and cholesterol supplies on the growth, reproduction, and survival of the land snail Arianta arbustorum. Surprisingly, differences in diet did not affect shell growth, time to reach adulthood, and reproductive traits such as the number of eggs, dry mass and volume of spermatophores. However, snails reared on low cholesterol diets and low polyunsaturated fatty acid (PUFA) diets decreased their consumption rates and had a higher mortality. Snails fed a PUFA-rich diet showed higher mating activity than snails fed a diet deficient in PUFAs. A depletion of internal PUFA reserves may affect the regulation of mating behaviour because PUFAs play an important role as precursors for signal-transduction involved in the regulation of mating and reproduction. In contrast, when the diets were deficient in cholesterol, mating activity was not affected},
  language  = {en}
}
@article{BroseTielboerger2005,
  author    = {Brose, Ulrich and Tielb{\"o}rger, Katja},
  title     = {Subtle differences in environmental stress along a flooding gradient affect the importance of inter-specific competition in an annual plant community},
  issn      = {1385-0237},
  year      = {2005},
  abstract  = {Empirical evidence suggests that the direction and intensity of plant-plant interactions may depend on the favourability of the environment. Previous studies have mainly focused on steep gradients of environmental stress or disturbance, while the interplay of competition and environment has not been tested for subtle environmental differences. Here, we present results from a study on plant communities of temporary wetlands in East-German farmland. Due to yearly ploughing in autumn, the vegetation is composed of annual species. Flooding does not affect adult plants and the elevation on the gradient expresses differences in the length of the growing season rather than in disturbance intensity or severe environmental stress. We tested whether such subtle differences in environmental stress may affect the importance of interspecific competition by the dominant species. Two treatments were applied at two elevations: removal of the dominant species (Matricaria maritima ssp. inodora) and reciprocal transplants of the seed-bank of the two elevations. At both elevations, removal of Matricaria inodora led to an increase in total species richness and number of wetland species, but the effects were substantially stronger at high elevations. Removal and the elevation on the flooding gradient significantly influenced the plant community composition. In particular, the weed communities became more similar to the wetland communities after the removal. Transplanted weed species did not emerge at low elevations. While two of four target species had significantly higher densities after the removal at high elevations, none of them was influenced by removal at low elevations. This indicates that, consistent with previous studies from other habitat types, competition by the dominant species was more intense under conditions of low environmental stress. The overall results suggest that both flooding as well as interspecific competition are important in structuring the plant communities along the freshwater gradient studied},
  language  = {en}
}
@article{ForlaniCeredaFreueretal.2005,
  author    = {Forlani, Fabio and Cereda, Angelo and Freuer, Andrea and Nimtz, Manfred and Leimk{\"u}hler, Silke and Pagani, Silvia},
  title     = {The cysteine-desulfurase IscS promotes the production of the rhodanese RhdA in the persulfurated form},
  issn      = {0014-5793},
  year      = {2005},
  abstract  = {After heterologous expression in Escherichia coli, the Azotobacter vinelandii rhodanese RhdA is purified in a persulfurated form (RhdA-SSH). We identified L-cysteine as the most effective sulfur source in producing RhdA-SSH. An E. coli soluble extract was required for in vitro persulfuration of RhdA, and the addition of pyridoxal-5'-phosphate increased RhdA-SSH production, indicating a likely involvement of a cysteine desulfurase. We were able to show the formation of a covalent complex between IscS and RhdA. By combining a time-course fluorescence assay and mass spectrometry analysis, we demonstrated the transfer of sulfur from E. coli IscS to RhdA. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved},
  language  = {en}
}
@article{WoltersBittmannKummer2005,
  author    = {Wolters, Steffen and Bittmann, Felix and Kummer, Volker},
  title     = {The first subfossil records of Urtica kioviensis Rogow. and their consequences for palaeoecological interpretations},
  year      = {2005},
  abstract  = {Among plant remains from Mesolithic layers dating from 9249 to 7779 B.C. at the excavation site of Friesack IV in north-eastern Germany, nutlets of Urtica kioviensis were identified. Morphological studies have shown that they clearly differed from all other European Urtica species investigated. In contrast, pollen morphological investigations revealed only slight differences between the central European Urtica species, which could hardly have been noticed during routine or normal pollen analyses. The records of U. kioviensis nutlets are the first subfossil finds reported and prove the indigenous status of this taxon in north-eastern Germany. The records are discussed in the context of the overall species spectrum of the Mesolithic layers and consequences for the interpretation of pollen analytical studies concerning human impact are pointed out},
  language  = {en}
}
@article{HeiseBier2005,
  author    = {Heise, Christian and Bier, Frank Fabian},
  title     = {Immobilization of DNA on microarrays},
  year      = {2005},
  abstract  = {Microarrays are new analytical devices that allow the parallel and simultaneous detection of thousands of target compounds. Microarrays, also called DNA chips, are widely used in gene expression, the genotyping of individuals, point mutations, detection of single nucleotide polymorphisms, and short tandem repeats. Microarrays have highly specific base-pair interactions with labeled complementary strands, which makes this technology to a powerful analytical device for monitoring whole genomes. In this article, we provide a survey of the common microarray manufacturing methods, from the selection of support material to surface structuring, immobilization and hybridization, and finally the detection with labeled complementary strands. Special attention is given to the immobilization of single strands, since fast chemical reactions, the creation of homogeneous surface functionalities as well as an oriented coupling are crucial pre-conditions for a good spot morphology and microarrays of high quality},
  language  = {en}
}
@article{FischerWipfRixenetal.2005,
  author    = {Fischer, Markus and Wipf, S. and Rixen, C. and St{\"o}ckli, V.},
  title     = {Effects of ski piste preparation on alpine vegetation},
  issn      = {0021-8901},
  year      = {2005},
  abstract  = {1. Ski resorts increasingly affect alpine ecosystems through enlargement of ski pistes, machine-grading of ski piste areas and increasing use of artificial snow. 2. In 12 Swiss alpine ski resorts, we investigated the effects of ski piste management on vegetation structure and composition using a pairwise design of 38 plots on ski pistes and 38 adjacent plots off-piste. 3. Plots on ski pistes had lower species richness and productivity, and lower abundance and cover of woody plants and early flowering species, than reference plots. Plots on machine-graded pistes had higher indicator values for nutrients and light, and lower vegetation cover, productivity, species diversity and abundance of early flowering and woody plants. Time since machine-grading did not mitigate the impacts of machine-grading, even for those plots where revegetation had been attempted by sowing. 4. The longer artificial snow had been used on ski pistes (2-15 years), the higher the moisture and nutrient indicator values. Longer use also affected species composition by increasing the abundance of woody plants, snowbed species and late-flowering species, and decreasing wind-edge species. 5. Synthesis and applications. All types of ski piste management cause deviations from the natural structure and composition of alpine vegetation, and lead to lower plant species diversity. Machine-grading causes particularly severe and lasting impacts on alpine vegetation, which are mitigated neither by time nor by revegetation measures. The impacts of artificial snow increase with the period of time since it was first applied to ski piste vegetation. Extensive machine-grading and snow production should be avoided, especially in areas where nutrient and water input are a concern. Ski pistes should not be established in areas where the alpine vegetation has a high conservation value},
  language  = {en}
}
@article{BolayBraunDelheyetal.2005,
  author    = {Bolay, Adrien and Braun, Uwe and Delhey, Rolf and Kummer, Volker and Piatek, Marcin and Wolczanska, Agata},
  title     = {Erysiphe deutziae : a new epidemic spread in Europe},
  year      = {2005},
  abstract  = {Erysiphe deutziae (Bunkina) U. Braun \& S. Takam. is powdery mildew fungus that is currently spreading in Europe. The anamorph of this species has been found in France, Germany, Poland and Switzerland on Deutzia sp. (cult.), Deutzia x magnifica (Lemoine) Rehder and Deutzia scabra Thunb. The morphology, taxonomy and worldwide distribution of Erysiphe deutziae are described, illustrated and discussed},
  language  = {en}
}
@article{SchellerBistolasLiuetal.2005,
  author    = {Scheller, Frieder W. and Bistolas, Nikitas and Liu, Songqin and J{\"a}nchen, Michael and Katterle, Martin and Wollenberger, Ursula},
  title     = {Thirty years of haemoglobin electrochemistry},
  year      = {2005},
  abstract  = {Electrochemical investigations of the blood oxygen carrier protein include both mediated and direct electron transfer. The reaction of haemoglobin (Hb) with typical mediators, e.g., ferricyanide, can be quantified by measuring the produced ferrocyanide which is equivalent to the Hb concentration. Immobilization of the mediator within the electrode body allows reagentless electrochemical measuring of Hb. On the other hand, entrapment of the protein within layers of polyclectrolytes, lipids, nanoparticles of clay or gold leads to a fast heterogeneous electron exchange of the partially denatured Hb. (c) 2005 Elsevier B.V. All rights reserved},
  language  = {en}
}
@article{Blenau2005,
  author    = {Blenau, Wolfgang},
  title     = {Cellular actions of biogenic amines},
  year      = {2005},
  language  = {en}
}
@article{MustardKurshanHamiltonetal.2005,
  author    = {Mustard, J. A. and Kurshan, P. T. and Hamilton, Ingrid S. and Blenau, Wolfgang and Mercer, Alison R.},
  title     = {Developmental expression of a tyramine receptor gene in the brain of the honey bee, Apis mellifera},
  year      = {2005},
  language  = {en}
}
@article{WichmannGroeneveldJeltschetal.2005,
  author    = {Wichmann, Matthias and Groeneveld, J{\"u}rgen and Jeltsch, Florian and Grimm, Volker},
  title     = {Mitigation of climate change impacts on raptors by behavioural adaption : ecological buffering mechanism},
  year      = {2005},
  language  = {en}
}
@article{WilliFischer2005,
  author    = {Willi, Yvonne and Fischer, Markus},
  title     = {Genetic rescue effect in interconnected populations of small and large size of the self-incompatible Ranunculus reptans},
  year      = {2005},
  language  = {en}
}
@article{FussmannBlasius2005,
  author    = {Fussmann, Gregor F. and Blasius, Bernd},
  title     = {Community response to enrichment is highly sensitive to model structure},
  year      = {2005},
  abstract  = {Biologists use mathematical functions to model, understand, and predict nature. For most biological processes, however, the exact analytical form is not known. This is also true for one of the most basic life processes, the uptake of food or resources. We show that the use of a number of nearly indistinguishable functions, which can serve as phenomenological descriptors of resource uptake, may lead to alarmingly different dynamical behaviour in a simple community model. More specifically, we demonstrate that the degree of resource enrichment needed to destabilize the community dynamics depends critically on the mathematical nature of the uptake function.},
  language  = {en}
}
@article{JeltschGroeneveldWisseletal.2005,
  author    = {Jeltsch, Florian and Groeneveld, J{\"u}rgen and Wissel, Christian and Wucherer, W. and Dimeyeva, L.},
  title     = {Seed dispersal by cattle may cause shrub encroachment of Grewia flava on southern Kalahari rangelands},
  isbn      = {3-86537-386-0},
  year      = {2005},
  language  = {en}
}
@article{FischerPerretGaleuchet2005,
  author    = {Fischer, Markus and Perret, Catherine and Galeuchet, David J.},
  title     = {Performance of Lychnis flos-cuculi from fragmented populations under experimental competition and pathogen infection, Ecology},
  issn      = {0012-9658},
  year      = {2005},
  language  = {en}
}
@article{SchmollDietrichWinkeletal.2005,
  author    = {Schmoll, Tim and Dietrich, Volkmar and Winkel, Wolfgang and Epplen, J. T. and Schurr, Frank Martin and Lubjuhn, Thomas},
  title     = {Paternal genetic effects on offspring fitness are context dependent within the extrapair mating system of a socially monogamous passerine},
  year      = {2005},
  abstract  = {Avian extrapair mating systems provide an interesting model to assess the role of genetic benefits in the evolution of female multiple mating behavior, as potentially confounding nongenetic benefits of extrapair mate choice are seen to be of minor importance. Genetic benefit models of extrapair mating behavior predict that females engage in extrapair copulations with males of higher genetic quality compared to their social mates, thereby improving offspring reproductive value. The most straightforward test of such good genes models of extrapair mating implies pail-wise comparisons of maternal half-siblings raised in the same environment, which permits direct assessment of Paternal genetic effects oil offspring traits. But genetic benefits of mate choice may be difficult to detect. Furthermore, the extent of genetic benefits (in terms of increased offspring viability or fecundity) may depend oil the environmental context Such that the proposed differences between extrapair offspring (EPO) and within-pair offspring (WPO) only appear under comparatively poor environmental conditions. We tested the hypothesis that genetic benefits of female extrapair mate choice are context dependent by analyzing offspring fitness-related traits in the coal tit (Parus ater) in relation to seasonal variation in environmental conditions. Paternal genetic effects on offspring fitness were context dependent. as shown by a significant interaction effect of differential paternal genetic contribution and offspring hatching date. EPO showed a higher local recruitment probability than their maternal half-siblings if born comparatively late in the season (i.e.. when overall performance had significantly declined), while WPO performed better early in the season. The same general pattern of context dependence was evident when using the number of grandchildren born to a cuckolding female via her female WPO or EPO progeny as the respective fitness measure. However, we were unable to demonstrate that cuckolding females obtained a general genetic fitness benefit from extrapair fertilizations in terms of offspring viability or fecundity. Thus, another type of benefit Could be responsible for maintaining female extrapair mating preferences in the study population. Our results suggest that more than a single selective pressure may have shaped the evolution of female extrapair mating behavior in socially monogamous passerines.},
  language  = {en}
}