@article{VandenWyngaertRojasJimenezSetoetal.2018, author = {Van den Wyngaert, Silke and Rojas-Jimenez, Keilor and Seto, Kensuke and Kagami, Maiko and Grossart, Hans-Peter}, title = {Diversity and Hidden Host Specificity of Chytrids Infecting Colonial Volvocacean Algae}, series = {Journal of Eukaryotic Microbiology}, volume = {65}, journal = {Journal of Eukaryotic Microbiology}, number = {6}, publisher = {Wiley}, address = {Hoboken}, issn = {1066-5234}, doi = {10.1111/jeu.12632}, pages = {870 -- 881}, year = {2018}, abstract = {Chytrids are zoosporic fungi that play an important, but yet understudied, ecological role in aquatic ecosystems. Many chytrid species have been morphologically described as parasites on phytoplankton. However, the majority of them have rarely been isolated and lack DNA sequence data. In this study we isolated and cultivated three parasitic chytrids, infecting a common volvocacean host species, Yamagishiella unicocca. To identify the chytrids, we characterized morphology and life cycle, and analyzed phylogenetic relationships based on 18S and 28S rDNA genes. Host range and specificity of the chytrids was determined by cross-infection assays with host strains, characterized by rbcL and ITS markers. We were able to confirm the identity of two chytrid strains as Endocoenobium eudorinae Ingold and Dangeardia mamillata Schroder and described the third chytrid strain as Algomyces stechlinensis gen. et sp. nov. The three chytrids were assigned to novel and phylogenetically distant clades within the phylum Chytridiomycota, each exhibiting different host specificities. By integrating morphological and molecular data of both the parasitic chytrids and their respective host species, we unveiled cryptic host-parasite associations. This study highlights that a high prevalence of (pseudo)cryptic diversity requires molecular characterization of both phytoplankton host and parasitic chytrid to accurately identify and compare host range and specificity, and to study phytoplankton-chytrid interactions in general.}, language = {en} } @article{AriasAndresKettnerMikietal.2018, author = {Arias Andr{\´e}s, Mar{\´i}a de Jes{\´u}s and Kettner, Marie Therese and Miki, Takeshi and Grossart, Hans-Peter}, title = {Microplastics: New substrates for heterotrophic activity contribute to altering organic matter cycles in aquatic ecosystems}, series = {The science of the total environment : an international journal for scientific research into the environment and its relationship with man}, volume = {635}, journal = {The science of the total environment : an international journal for scientific research into the environment and its relationship with man}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0048-9697}, doi = {10.1016/j.scitotenv.2018.04.199}, pages = {1152 -- 1159}, year = {2018}, abstract = {Heterotrophic microbes with the capability to process considerable amounts of organic matter can colonize microplastic particles (MP) in aquatic ecosystems. Weather colonization of microorganisms on MP will alter ecological niche and functioning of microbial communities remains still unanswered. Therefore, we compared the functional diversity of biofilms on microplastics when incubated in three lakes in northeastern Germany differing in trophy and limnological features. For all lakes, we compared heterotrophic activities of MP biofilms with those of microorganisms in the surrounding water by using Biolog (R) EcoPlates and assessed their oxygen consumption in microcosm assays with and without MP. The present study found that the total biofilm biomass was higher in the oligo-mesotrophic and dystrophic lakes than in the eutrophic lake. In all lakes, functional diversity profiles of MP biofilms consistently differed from those in the surrounding water. However, solely in the oligo-mesotrophic lake MP biofilms had a higher functional richness compared to the ambient water. These results demonstrate that the functionality and hence the ecological role of MP-associated microbial communities are context-dependent, i.e. different environments lead to substantial changes in biomass build up and heterotrophic activities of MP biofilms. We propose that MP surfaces act as new niches for aquatic microorganisms and that the constantly increasing MP pollution has the potential to globally impact carbon dynamics of pelagic environments by altering heterotrophic activities. (C) 2018 Elsevier B.V. All rights reserved.}, language = {en} } @article{MantzoukiBekliogluBrookesetal.2018, author = {Mantzouki, Evanthia and Beklioglu, Meryem and Brookes, Justin D. and Domis, Lisette Nicole de Senerpont and Dugan, Hilary A. and Doubek, Jonathan P. and Grossart, Hans-Peter and Nejstgaard, Jens C. and Pollard, Amina I. and Ptacnik, Robert and Rose, Kevin C. and Sadro, Steven and Seelen, Laura and Skaff, Nicholas K. and Teubner, Katrin and Weyhenmeyer, Gesa A. and Ibelings, Bastiaan W.}, title = {Snapshot surveys for lake monitoring, more than a shot in the dark}, series = {Frontiers in Ecology and Evolution}, volume = {6}, journal = {Frontiers in Ecology and Evolution}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {2296-701X}, doi = {10.3389/fevo.2018.00201}, pages = {5}, year = {2018}, language = {en} } @article{MantzoukiCampbellvanLoonetal.2018, author = {Mantzouki, Evanthia and Campbell, James and van Loon, Emiel and Visser, Petra and Konstantinou, Iosif and Antoniou, Maria and Giuliani, Gregory and Machado-Vieira, Danielle and de Oliveira, Alinne Gurjao and Maronic, Dubravka Spoljaric and Stevic, Filip and Pfeiffer, Tanja Zuna and Vucelic, Itana Bokan and Zutinic, Petar and Udovic, Marija Gligora and Plenkovic-Moraj, Andelka and Tsiarta, Nikoletta and Blaha, Ludek and Geris, Rodan and Frankova, Marketa and Christoffersen, Kirsten Seestern and Warming, Trine Perlt and Feldmann, Tonu and Laas, Alo and Panksep, Kristel and Tuvikene, Lea and Kangro, Kersti and Haggqvist, Kerstin and Salmi, Pauliina and Arvola, Lauri and Fastner, Jutta and Straile, Dietmar and Rothhaupt, Karl-Otto and Fonvielle, Jeremy Andre and Grossart, Hans-Peter and Avagianos, Christos and Kaloudis, Triantafyllos and Triantis, Theodoros and Zervou, Sevasti-Kiriaki and Hiskia, Anastasia and Gkelis, Spyros and Panou, Manthos and McCarthy, Valerie and Perello, Victor C. and Obertegger, Ulrike and Boscaini, Adriano and Flaim, Giovanna and Salmaso, Nico and Cerasino, Leonardo and Koreiviene, Judita and Karosiene, Jurate and Kasperoviciene, Jurate and Savadova, Ksenija and Vitonyte, Irma and Haande, Sigrid and Skjelbred, Birger and Grabowska, Magdalena and Karpowicz, Maciej and Chmura, Damian and Nawrocka, Lidia and Kobos, Justyna and Mazur-Marzec, Hanna and Alcaraz-Parraga, Pablo and Wilk-Wozniak, Elzbieta and Krzton, Wojciech and Walusiak, Edward and Gagala, Ilona and Mankiewicz-Boczek, Joana and Toporowska, Magdalena and Pawlik-Skowronska, Barbara and Niedzwiecki, Michal and Peczula, Wojciech and Napiorkowska-Krzebietke, Agnieszka and Dunalska, Julita and Sienska, Justyna and Szymanski, Daniel and Kruk, Marek and Budzynska, Agnieszka and Goldyn, Ryszard and Kozak, Anna and Rosinska, Joanna and Szelag-Wasielewska, Elzbieta and Domek, Piotr and Jakubowska-Krepska, Natalia and Kwasizur, Kinga and Messyasz, Beata and Pelechata, Aleksandra and Pelechaty, Mariusz and Kokocinski, Mikolaj and Madrecka, Beata and Kostrzewska-Szlakowska, Iwona and Frak, Magdalena and Bankowska-Sobczak, Agnieszka and Wasilewicz, Michal and Ochocka, Agnieszka and Pasztaleniec, Agnieszka and Jasser, Iwona and Antao-Geraldes, Ana M. and Leira, Manel and Hernandez, Armand and Vasconcelos, Vitor and Morais, Joao and Vale, Micaela and Raposeiro, Pedro M. and Goncalves, Vitor and Aleksovski, Boris and Krstic, Svetislav and Nemova, Hana and Drastichova, Iveta and Chomova, Lucia and Remec-Rekar, Spela and Elersek, Tina and Delgado-Martin, Jordi and Garcia, David and Luis Cereijo, Jose and Goma, Joan and Carmen Trapote, Mari and Vegas-Vilarrubia, Teresa and Obrador, Biel and Garcia-Murcia, Ana and Real, Monserrat and Romans, Elvira and Noguero-Ribes, Jordi and Parreno Duque, David and Fernandez-Moran, Elisabeth and Ubeda, Barbara and Angel Galvez, Jose and Marce, Rafael and Catalan, Nuria and Perez-Martinez, Carmen and Ramos-Rodriguez, Eloisa and Cillero-Castro, Carmen and Moreno-Ostos, Enrique and Maria Blanco, Jose and Rodriguez, Valeriano and Juan Montes-Perez, Jorge and Palomino, Roberto L. and Rodriguez-Perez, Estela and Carballeira, Rafael and Camacho, Antonio and Picazo, Antonio and Rochera, Carlos and Santamans, Anna C. and Ferriol, Carmen and Romo, Susana and Soria, Juan Miguel and Hansson, Lars-Anders and Urrutia-Cordero, Pablo and Ozen, Arda and Bravo, Andrea G. and Buck, Moritz and Colom-Montero, William and Mustonen, Kristiina and Pierson, Don and Yang, Yang and Verspagen, Jolanda M. H. and Domis, Lisette N. de Senerpont and Seelen, Laura and Teurlincx, Sven and Verstijnen, Yvon and Lurling, Miquel and Maliaka, Valentini and Faassen, Elisabeth J. and Latour, Delphine and Carey, Cayelan C. and Paerl, Hans W. and Torokne, Andrea and Karan, Tunay and Demir, Nilsun and Beklioglu, Meryem and Filiz, Nur and Levi, Eti E. and Iskin, Ugur and Bezirci, Gizem and Tavsanoglu, Ulku Nihan and Celik, Kemal and Ozhan, Koray and Karakaya, Nusret and Kocer, Mehmet Ali Turan and Yilmaz, Mete and Maraslioglu, Faruk and Fakioglu, Ozden and Soylu, Elif Neyran and Yagci, Meral Apaydin and Cinar, Sakir and Capkin, Kadir and Yagci, Abdulkadir and Cesur, Mehmet and Bilgin, Fuat and Bulut, Cafer and Uysal, Rahmi and Koker, Latife and Akcaalan, Reyhan and Albay, Meric and Alp, Mehmet Tahir and Ozkan, Korhan and Sevindik, Tugba Ongun and Tunca, Hatice and Onem, Burcin and Richardson, Jessica and Edwards, Christine and Bergkemper, Victoria and Beirne, Eilish and Cromie, Hannah and Ibelings, Bastiaan W.}, title = {Data Descriptor: A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins}, series = {Scientific Data}, volume = {5}, journal = {Scientific Data}, publisher = {Nature Publ. Group}, address = {London}, issn = {2052-4463}, doi = {10.1038/sdata.2018.226}, pages = {13}, year = {2018}, abstract = {Under ongoing climate change and increasing anthropogenic activity, which continuously challenge ecosystem resilience, an in-depth understanding of ecological processes is urgently needed. Lakes, as providers of numerous ecosystem services, face multiple stressors that threaten their functioning. Harmful cyanobacterial blooms are a persistent problem resulting from nutrient pollution and climate-change induced stressors, like poor transparency, increased water temperature and enhanced stratification. Consistency in data collection and analysis methods is necessary to achieve fully comparable datasets and for statistical validity, avoiding issues linked to disparate data sources. The European Multi Lake Survey (EMLS) in summer 2015 was an initiative among scientists from 27 countries to collect and analyse lake physical, chemical and biological variables in a fully standardized manner. This database includes in-situ lake variables along with nutrient, pigment and cyanotoxin data of 369 lakes in Europe, which were centrally analysed in dedicated laboratories. Publishing the EMLS methods and dataset might inspire similar initiatives to study across large geographic areas that will contribute to better understanding lake responses in a changing environment.}, language = {en} } @article{DarwallBremerichDeWeveretal.2018, author = {Darwall, William and Bremerich, Vanessa and De Wever, Aaike and Dell, Anthony I. and Freyhof, Joerg and Gessner, Mark O. and Grossart, Hans-Peter and Harrison, Ian and Irvine, Ken and J{\"a}hnig, Sonja C. and Jeschke, Jonathan M. and Lee, Jessica J. and Lu, Cai and Lewandowska, Aleksandra M. and Monaghan, Michael T. and Nejstgaard, Jens C. and Patricio, Harmony and Schmidt-Kloiber, Astrid and Stuart, Simon N. and Thieme, Michele and Tockner, Klement and Turak, Eren and Weyl, Olaf}, title = {The alliance for freshwater life}, series = {Aquatic Conservation: Marine and Freshwater Ecosystems}, volume = {28}, journal = {Aquatic Conservation: Marine and Freshwater Ecosystems}, number = {4}, publisher = {Wiley}, address = {Hoboken}, issn = {1052-7613}, doi = {10.1002/aqc.2958}, pages = {1015 -- 1022}, year = {2018}, abstract = {1. Global pressures on freshwater ecosystems are high and rising. Viewed primarily as a resource for humans, current practices of water use have led to catastrophic declines in freshwater species and the degradation of freshwater ecosystems, including their genetic and functional diversity. Approximately three-quarters of the world's inland wetlands have been lost, one-third of the 28 000 freshwater species assessed for the International Union for Conservation of Nature (IUCN) Red List are threatened with extinction, and freshwater vertebrate populations are undergoing declines that are more rapid than those of terrestrial and marine species. This global loss continues unchecked, despite the importance of freshwater ecosystems as a source of clean water, food, livelihoods, recreation, and inspiration. 2. The causes of these declines include hydrological alterations, habitat degradation and loss, overexploitation, invasive species, pollution, and the multiple impacts of climate change. Although there are policy initiatives that aim to protect freshwater life, these are rarely implemented with sufficient conviction and enforcement. Policies that focus on the development and management of fresh waters as a resource for people almost universally neglect the biodiversity that they contain. 3. Here we introduce the Alliance for Freshwater Life, a global initiative, uniting specialists in research, data synthesis, conservation, education and outreach, and policymaking. This expert network aims to provide the critical mass required for the effective representation of freshwater biodiversity at policy meetings, to develop solutions balancing the needs of development and conservation, and to better convey the important role freshwater ecosystems play in human well-being. Through this united effort we hope to reverse this tide of loss and decline in freshwater biodiversity. We introduce several short- and medium-term actions as examples for making positive change, and invite individuals, organizations, authorities, and governments to join the Alliance for Freshwater Life.}, language = {en} } @article{GoeritzBergerGegeetal.2018, author = {G{\"o}ritz, Anna and Berger, Stella A. and Gege, Peter and Grossart, Hans-Peter and Nejstgaard, Jens C. and Riedel, Sebastian and R{\"o}ttgers, R{\"u}diger and Utschig, Christian}, title = {Retrieval of water constituents from hyperspectral in-situ measurements under variable cloud cover}, series = {Remote sensing / Molecular Diversity Preservation International (MDPI)}, volume = {10}, journal = {Remote sensing / Molecular Diversity Preservation International (MDPI)}, number = {2}, publisher = {MDPI}, address = {Basel}, issn = {2072-4292}, doi = {10.3390/rs10020181}, pages = {19}, year = {2018}, abstract = {Remote sensing and field spectroscopy of natural waters is typically performed under clear skies, low wind speeds and low solar zenith angles. Such measurements can also be made, in principle, under clouds and mixed skies using airborne or in-situ measurements; however, variable illumination conditions pose a challenge to data analysis. In the present case study, we evaluated the inversion of hyperspectral in-situ measurements for water constituent retrieval acquired under variable cloud cover. First, we studied the retrieval of Chlorophyll-a (Chl-a) concentration and colored dissolved organic matter (CDOM) absorption from in-water irradiance measurements. Then, we evaluated the errors in the retrievals of the concentration of total suspended matter (TSM), Chl-a and the absorption coefficient of CDOM from above-water reflectance measurements due to highly variable reflections at the water surface. In order to approximate cloud reflections, we extended a recent three-component surface reflectance model for cloudless atmospheres by a constant offset and compared different surface reflectance correction procedures. Our findings suggest that in-water irradiance measurements may be used for the analysis of absorbing compounds even under highly variable weather conditions. The extended surface reflectance model proved to contribute to the analysis of above-water reflectance measurements with respect to Chl-a and TSM. Results indicate the potential of this approach for all-weather monitoring.}, language = {en} } @article{CuadratIonescuDavilaetal.2018, author = {Cuadrat, Rafael R. C. and Ionescu, Danny and Davila, Alberto M. R. and Grossart, Hans-Peter}, title = {Recovering genomics clusters of secondary metabolites from lakes using genome-resolved metagenomics}, series = {Frontiers in microbiology}, volume = {9}, journal = {Frontiers in microbiology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-302X}, doi = {10.3389/fmicb.2018.00251}, pages = {13}, year = {2018}, abstract = {Metagenomic approaches became increasingly popular in the past decades due to decreasing costs of DNA sequencing and bioinformatics development. So far, however, the recovery of long genes coding for secondary metabolites still represents a big challenge. Often, the quality of metagenome assemblies is poor, especially in environments with a high microbial diversity where sequence coverage is low and complexity of natural communities high. Recently, new and improved algorithms for binning environmental reads and contigs have been developed to overcome such limitations. Some of these algorithms use a similarity detection approach to classify the obtained reads into taxonomical units and to assemble draft genomes. This approach, however, is quite limited since it can classify exclusively sequences similar to those available (and well classified) in the databases. In this work, we used draft genomes from Lake Stechlin, north-eastern Germany, recovered by MetaBat, an efficient binning tool that integrates empirical probabilistic distances of genome abundance, and tetranucleotide frequency for accurate metagenome binning. These genomes were screened for secondary metabolism genes, such as polyketide synthases (PKS) and non-ribosomal peptide synthases (NRPS), using the Anti-SMASH and NAPDOS workflows. With this approach we were able to identify 243 secondary metabolite clusters from 121 genomes recovered from our lake samples. A total of 18 NRPS, 19 PKS, and 3 hybrid PKS/NRPS clusters were found. In addition, it was possible to predict the partial structure of several secondary metabolite clusters allowing for taxonomical classifications and phylogenetic inferences. Our approach revealed a high potential to recover and study secondary metabolites genes from any aquatic ecosystem.}, language = {en} } @article{GanghofEppnerPoerschke2018, author = {Ganghof, Steffen and Eppner, Sebastian and P{\"o}rschke, Alexander}, title = {Semi-parliamentary government in perspective}, series = {Australian Journal of Political Science}, volume = {53}, journal = {Australian Journal of Political Science}, number = {2}, publisher = {Routledge, Taylor \& Francis Group}, address = {Abingdon}, issn = {1036-1146}, doi = {10.1080/10361146.2018.1451488}, pages = {264 -- 269}, year = {2018}, abstract = {The article responds to four commentaries on the concept of semi-parliamentary government and its application to Australian bicameralism. It highlights four main points: (1) Our preferred typology is not more 'normative' than existing approaches, but applies the criterion of 'direct election' equally to executive and legislature; (2) While the evolution of semi-parliamentary government had contingent elements, it plausibly also reflects the 'equilibrium' nature of certain institutional configurations; (3) The idea that a pure parliamentary system with pure proportional representation has absolute normative priority over 'instrumentalist' concerns about cabinet stability, identifiability and responsibility is questionable; and (4) The reforms we discuss may be unlikely to occur in Australia, but deserve consideration by scholars and institutional reformers in other democratic systems.}, language = {en} } @article{GarciaBuckHamiltonetal.2018, author = {Garcia, Sarahi L. and Buck, Moritz and Hamilton, Joshua J. and Wurzbacher, Christian and Grossart, Hans-Peter and McMahon, Katherine D. and Eiler, Alexander}, title = {Model communities hint at promiscuous metabolic linkages between ubiquitous free-living freshwater bacteria}, series = {mSphere}, volume = {3}, journal = {mSphere}, number = {3}, publisher = {American Society for Microbiology}, address = {Washington}, issn = {2379-5042}, doi = {10.1128/mSphere.00202-18}, pages = {8}, year = {2018}, abstract = {Genome streamlining is frequently observed in free-living aquatic microorganisms and results in physiological dependencies between microorganisms. However, we know little about the specificity of these microbial associations. In order to examine the specificity and extent of these associations, we established mixed cultures from three different freshwater environments and analyzed the cooccurrence of organisms using a metagenomic time series. Free-living microorganisms with streamlined genomes lacking multiple biosynthetic pathways showed no clear recurring pattern in their interaction partners. Free-living freshwater bacteria form promiscuous cooperative associations. This notion contrasts with the well-documented high specificities of interaction partners in host-associated bacteria. Considering all data together, we suggest that highly abundant free-living bacterial lineages are functionally versatile in their interactions despite their distinct streamlining tendencies at the single-cell level. This metabolic versatility facilitates interactions with a variable set of community members.}, language = {en} } @article{MantzoukiLurlingFastneretal.2018, author = {Mantzouki, Evanthia and Lurling, Miquel and Fastner, Jutta and Domis, Lisette Nicole de Senerpont and Wilk-Wozniak, Elzbieta and Koreiviene, Judita and Seelen, Laura and Teurlincx, Sven and Verstijnen, Yvon and Krzton, Wojciech and Walusiak, Edward and Karosiene, Jurate and Kasperoviciene, Jurate and Savadova, Ksenija and Vitonyte, Irma and Cillero-Castro, Carmen and Budzynska, Agnieszka and Goldyn, Ryszard and Kozak, Anna and Rosinska, Joanna and Szelag-Wasielewska, Elzbieta and Domek, Piotr and Jakubowska-Krepska, Natalia and Kwasizur, Kinga and Messyasz, Beata and Pelechata, Aleksandra and Pelechaty, Mariusz and Kokocinski, Mikolaj and Garcia-Murcia, Ana and Real, Monserrat and Romans, Elvira and Noguero-Ribes, Jordi and Parreno Duque, David and Fernandez-Moran, Elisabeth and Karakaya, Nusret and Haggqvist, Kerstin and Demir, Nilsun and Beklioglu, Meryem and Filiz, Nur and Levi, Eti E. and Iskin, Ugur and Bezirci, Gizem and Tavsanoglu, Ulku Nihan and Ozhan, Koray and Gkelis, Spyros and Panou, Manthos and Fakioglu, Ozden and Avagianos, Christos and Kaloudis, Triantafyllos and Celik, Kemal and Yilmaz, Mete and Marce, Rafael and Catalan, Nuria and Bravo, Andrea G. and Buck, Moritz and Colom-Montero, William and Mustonen, Kristiina and Pierson, Don and Yang, Yang and Raposeiro, Pedro M. and Goncalves, Vitor and Antoniou, Maria G. and Tsiarta, Nikoletta and McCarthy, Valerie and Perello, Victor C. and Feldmann, Tonu and Laas, Alo and Panksep, Kristel and Tuvikene, Lea and Gagala, Ilona and Mankiewicz-Boczek, Joana and Yagci, Meral Apaydin and Cinar, Sakir and Capkin, Kadir and Yagci, Abdulkadir and Cesur, Mehmet and Bilgin, Fuat and Bulut, Cafer and Uysal, Rahmi and Obertegger, Ulrike and Boscaini, Adriano and Flaim, Giovanna and Salmaso, Nico and Cerasino, Leonardo and Richardson, Jessica and Visser, Petra M. and Verspagen, Jolanda M. H. and Karan, Tunay and Soylu, Elif Neyran and Maraslioglu, Faruk and Napiorkowska-Krzebietke, Agnieszka and Ochocka, Agnieszka and Pasztaleniec, Agnieszka and Antao-Geraldes, Ana M. and Vasconcelos, Vitor and Morais, Joao and Vale, Micaela and Koker, Latife and Akcaalan, Reyhan and Albay, Meric and Maronic, Dubravka Spoljaric and Stevic, Filip and Pfeiffer, Tanja Zuna and Fonvielle, Jeremy Andre and Straile, Dietmar and Rothhaupt, Karl-Otto and Hansson, Lars-Anders and Urrutia-Cordero, Pablo and Blaha, Ludek and Geris, Rodan and Frankova, Marketa and Kocer, Mehmet Ali Turan and Alp, Mehmet Tahir and Remec-Rekar, Spela and Elersek, Tina and Triantis, Theodoros and Zervou, Sevasti-Kiriaki and Hiskia, Anastasia and Haande, Sigrid and Skjelbred, Birger and Madrecka, Beata and Nemova, Hana and Drastichova, Iveta and Chomova, Lucia and Edwards, Christine and Sevindik, Tugba Ongun and Tunca, Hatice and OEnem, Burcin and Aleksovski, Boris and Krstic, Svetislav and Vucelic, Itana Bokan and Nawrocka, Lidia and Salmi, Pauliina and Machado-Vieira, Danielle and de Oliveira, Alinne Gurjao and Delgado-Martin, Jordi and Garcia, David and Cereijo, Jose Luis and Goma, Joan and Trapote, Mari Carmen and Vegas-Vilarrubia, Teresa and Obrador, Biel and Grabowska, Magdalena and Karpowicz, Maciej and Chmura, Damian and Ubeda, Barbara and Angel Galvez, Jose and Ozen, Arda and Christoffersen, Kirsten Seestern and Warming, Trine Perlt and Kobos, Justyna and Mazur-Marzec, Hanna and Perez-Martinez, Carmen and Ramos-Rodriguez, Eloisa and Arvola, Lauri and Alcaraz-Parraga, Pablo and Toporowska, Magdalena and Pawlik-Skowronska, Barbara and Niedzwiecki, Michal and Peczula, Wojciech and Leira, Manel and Hernandez, Armand and Moreno-Ostos, Enrique and Maria Blanco, Jose and Rodriguez, Valeriano and Juan Montes-Perez, Jorge and Palomino, Roberto L. and Rodriguez-Perez, Estela and Carballeira, Rafael and Camacho, Antonio and Picazo, Antonio and Rochera, Carlos and Santamans, Anna C. and Ferriol, Carmen and Romo, Susana and Miguel Soria, Juan and Dunalska, Julita and Sienska, Justyna and Szymanski, Daniel and Kruk, Marek and Kostrzewska-Szlakowska, Iwona and Jasser, Iwona and Zutinic, Petar and Udovic, Marija Gligora and Plenkovic-Moraj, Andelka and Frak, Magdalena and Bankowska-Sobczak, Agnieszka and Wasilewicz, Michal and Ozkan, Korhan and Maliaka, Valentini and Kangro, Kersti and Grossart, Hans-Peter and Paerl, Hans W. and Carey, Cayelan C. and Ibelings, Bas W.}, title = {Temperature effects explain continental scale distribution of cyanobacterial toxins}, series = {Toxins}, volume = {10}, journal = {Toxins}, number = {4}, publisher = {MDPI}, address = {Basel}, issn = {2072-6651}, doi = {10.3390/toxins10040156}, pages = {24}, year = {2018}, abstract = {Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect effects of temperature were the main drivers of the spatial distribution in the toxins produced by the cyanobacterial community, the toxin concentrations and toxin quota. Generalized linear models showed that a Toxin Diversity Index (TDI) increased with latitude, while it decreased with water stability. Increases in TDI were explained through a significant increase in toxin variants such as MC-YR, anatoxin and cylindrospermopsin, accompanied by a decreasing presence of MC-LR. While global warming continues, the direct and indirect effects of increased lake temperatures will drive changes in the distribution of cyanobacterial toxins in Europe, potentially promoting selection of a few highly toxic species or strains.}, language = {en} } @article{SchulzeMakuchWagnerKounavesetal.2018, author = {Schulze-Makuch, Dirk and Wagner, Dirk and Kounaves, Samuel P. and Mangelsdorf, Kai and Devine, Kevin G. and de Vera, Jean-Pierre and Schmitt-Kopplin, Philippe and Grossart, Hans-Peter and Parro, Victor and Kaupenjohann, Martin and Galy, Albert and Schneider, Beate and Airo, Alessandro and Froesler, Jan and Davila, Alfonso F. and Arens, Felix L. and Caceres, Luis and Cornejo, Francisco Solis and Carrizo, Daniel and Dartnell, Lewis and DiRuggiero, Jocelyne and Flury, Markus and Ganzert, Lars and Gessner, Mark O. and Grathwohl, Peter and Guan, Lisa and Heinz, Jacob and Hess, Matthias and Keppler, Frank and Maus, Deborah and McKay, Christopher P. and Meckenstock, Rainer U. and Montgomery, Wren and Oberlin, Elizabeth A. and Probst, Alexander J. and Saenz, Johan S. and Sattler, Tobias and Schirmack, Janosch and Sephton, Mark A. and Schloter, Michael and Uhl, Jenny and Valenzuela, Bernardita and Vestergaard, Gisle and Woermer, Lars and Zamorano, Pedro}, title = {Transitory microbial habitat in the hyperarid Atacama Desert}, series = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {115}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, number = {11}, publisher = {National Acad. of Sciences}, address = {Washington}, issn = {0027-8424}, doi = {10.1073/pnas.1714341115}, pages = {2670 -- 2675}, year = {2018}, language = {en} } @article{BatistaWoodhouseGrossartetal.2018, author = {Batista, A. M. M. and Woodhouse, Jason Nicholas and Grossart, Hans-Peter and Giani, A.}, title = {Methanogenic archaea associated to Microcystis sp. in field samples and in culture}, series = {Hydrobiologia : acta hydrobiologica, hydrographica, limnologica et protistologica}, volume = {831}, journal = {Hydrobiologia : acta hydrobiologica, hydrographica, limnologica et protistologica}, number = {1}, publisher = {Springer}, address = {Dordrecht}, issn = {0018-8158}, doi = {10.1007/s10750-018-3655-3}, pages = {163 -- 172}, year = {2018}, abstract = {Cyanobacterial mass developments impact the community composition of heterotrophic microorganisms with far-reaching consequences for biogeochemical and energy cycles of freshwater ecosystems including reservoirs. Here we sought to evaluate the temporal stability of methanogenic archaea in the water column and further scrutinize their associations with cyanobacteria. Monthly samples were collected from October 2009 to December 2010 in hypereutrophic Pampulha reservoir with permanently blooming cyanobacteria, and from January to December 2011 in oligotrophic Volta Grande reservoir with only sporadic cyanobacteria incidence. The presence of archaea in cyanobacterial cultures was investigated by screening numerous strains of Microcystis spp. from these reservoirs as well as from lakes in Europe, Asia, and North-America. We consistently determined the occurrence of archaea, in particular methanogenic archaea, in both reservoirs throughout the year. However, archaea were only associated with two strains (Microcystis sp. UFMG 165 and UFMG 175) recently isolated from these reservoirs. These findings do not implicate archaea in the occurrence of methane in the epilimnion of inland waters, but rather serve to highlight the potential of microhabitats associated with particles, including phytoplankton, to shelter unique microbial communities.}, language = {en} } @article{MugelePlummerSteffenetal.2018, author = {Mugele, Hendrik and Plummer, Ashley and Steffen, Kathrin and Stoll, Josefine and Mayer, Frank and M{\"u}ller, Juliane}, title = {General versus sports-specific injury prevention programs in athletes}, series = {PLOS ONE}, volume = {13}, journal = {PLOS ONE}, number = {10}, publisher = {Public Library of Science}, address = {San Francisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0205635}, pages = {1 -- 16}, year = {2018}, abstract = {Introduction Annually, 2 million sports-related injuries are reported in Germany of which athletes contribute to a large proportion. Multiple sport injury prevention programs designed to decrease acute and overuse injuries in athletes have been proven effective. Yet, the programs' components, general or sports-specific, that led to these positive effects are uncertain. Despite not knowing about the superiority of sports-specific injury prevention programs, coaches and athletes alike prefer more specialized rather than generalized exercise programs. Therefore, this systematic review aimed to present the available evidence on how general and sports-specific prevention programs affect injury rates in athletes. Methods PubMed and Web of Science were electronically searched throughout April 2018. The inclusion criteria were publication dates Jan 2006-Dec 2017, athletes (11-45 years), exercise-based injury prevention programs and injury incidence. The methodological quality was assessed with the Cochrane Collaboration assessment tools. Results Of the initial 6619 findings, 15 studies met the inclusion criteria. In addition, 13 studies were added from reference lists and external sources making a total of 28 studies. Of which, one used sports-specific, seven general and 20 mixed prevention strategies. Twenty-four studies revealed reduced injury rates. Of the four ineffective programs, one was general and three mixed. Conclusion The general and mixed programs positively affect injury rates. Sports-specific programs are uninvestigated and despite wide discussion regarding the definition, no consensus was reached. Defining such terminology and investigating the true effectiveness of such IPPs is a potential avenue for future research.}, language = {en} } @article{RuszkiewiczdeMacedoMirandaVizueteetal.2018, author = {Ruszkiewicz, Joanna and de Macedo, Gabriel Teixeira and Miranda-Vizuete, Antonio and Teixeira da Rocha, Joao B. and Bowman, Aaron B. and Bornhorst, Julia and Schwerdtle, Tanja and Aschner, Michael}, title = {The cytoplasmic thioredoxin system in Caenorhabditis elegans affords protection from methylmercury in an age-specific manner}, series = {Neurotoxicology : the interdisciplinary journal of effects to toxic substances on the nervous system}, volume = {68}, journal = {Neurotoxicology : the interdisciplinary journal of effects to toxic substances on the nervous system}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0161-813X}, doi = {10.1016/j.neuro.2018.08.007}, pages = {189 -- 202}, year = {2018}, abstract = {Methylmercury (MeHg) is an environmental pollutant linked to many neurological defects, especially in developing individuals. The thioredoxin (TRX) system is a key redox regulator affected by MeHg toxicity, however the mechanisms and consequences of MeHg-induced dysfunction are not completely understood. This study evaluated the role of the TRX system in C. elegans susceptibility to MeHg during development. Worms lacking or overexpressing proteins from the TRX family were exposed to MeHg for 1 h at different developmental stage: L1, L4 and adult. Worms without cytoplasmic thioredoxin system exhibited age-specific susceptibility to MeHg when compared to wild-type (wt). This susceptibility corresponded partially to decreased total glutathione (GSH) levels and enhanced degeneration of dopaminergic neurons. In contrast, the overexpression of the cytoplasmic system TRX-1/TRXR-1 did not provide substantial protection against MeHg. Moreover, transgenic worms exhibited decreased protein expression for cytoplasmic thioredoxin reductase (TRXR-1). Both mitochondrial thioredoxin system TRX-2/TRXR-2, as well as other thioredoxin-like proteins: TRX-3, TRX-4, TRX-5 did not show significant role in C. elegans resistance to MeHg. Based on the current findings, the cytoplasmic thioredoxin system TRX-1/TRXR-1 emerges as an important age-sensitive protectant against MeHg toxicity in C. elegans.}, language = {en} } @article{BaroniScheiffeleSchroenetal.2018, author = {Baroni, Gabriele and Scheiffele, Lena and Schr{\"o}n, Martin and Ingwersen, Joachim and Oswald, Sascha}, title = {Uncertainty, sensitivity and improvements in soil moisture estimation with cosmic-ray neutron sensing}, series = {Journal of hydrology}, volume = {564}, journal = {Journal of hydrology}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0022-1694}, doi = {10.1016/j.jhydrol.2018.07.053}, pages = {873 -- 887}, year = {2018}, abstract = {Cosmic-ray neutron sensing (CRNS) is a promising proximal soil sensing technique to estimate soil moisture at intermediate scale and high temporal resolution. However, the signal shows complex and non-unique response to all hydrogen pools near the land surface, providing some challenges for soil moisture estimation in practical applications. Aims of the study were 1) to assess the uncertainty of CRNS as a stand-alone approach to estimate volumetric soil moisture in cropped field 2) to identify the causes of this uncertainty 3) and possible improvements. Two experimental sites in Germany were equipped with a CRNS probe and point-scale soil moisture network. Additional monitoring activities were conducted during the crop growing season to characterize the soil-plant systems. This data is used to identify and quantify the different sources of uncertainty (factors). An uncertainty analysis, based on Monte Carlo approach, is applied to propagate these uncertainties to CRNS soil moisture estimations. In addition, a sensitivity analysis based on the Sobol' method is performed to identify the most important factors explaining this uncertainty. Results show that CRNS soil moisture compares well to the soil moisture network when these point-scale values are weighted to account for the spatial sensitivity of the signal and other sources of hydrogen (lattice water and organic carbon) are added to the water content. However, the performance decreases when CRNS is considered as a stand-alone method to retrieve the actual (non-weighted) volumetric soil moisture. The support volume (penetration depth and radius) shows also a considerable uncertainty, especially in relatively dry soil moisture conditions. Four of the seven factors analyzed (the vertical soil moisture profile, bulk density, incoming neutron correction and the calibrated parameter N0) were found to play an important role. Among the possible improvements identified, a simple correction factor based on vertical point-scale soil moisture profiles shows to be a promising approach to account for the sensitivity of the CRNS signal to the upper soil layers.}, language = {en} }