@article{ShahidManchiSlunskyetal.2017, author = {Shahid, Muhammad and Manchi, G. and Slunsky, Pavel and Naseer, O. and Fatima, A. and Leo, B. and Raila, Jens}, title = {A systemic review of existing serological possibilities to diagnose canine osteoarthritis with a particular focus on extracellular matrix proteoglycans and protein}, series = {Polish journal of veterinary sciences : PJVS : the journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn}, volume = {20}, journal = {Polish journal of veterinary sciences : PJVS : the journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn}, number = {1}, publisher = {De Gruyter}, address = {Berlin}, issn = {1505-1773}, doi = {10.1515/pjvs-2017-0024}, pages = {189 -- 201}, year = {2017}, abstract = {Extra-cellular matrix (ECM) components are important and their stabilization is significant in maintaining normal healthy joint environment. In osteoarthritis (OA), ECM components are altered and indicate disease progression. The joint ECM is composed of proteoglycans (aggrecan, perlecan,inter α-trypsin inhibitor), glycoproteins (fibronectin, lubricin, COMP) and collagen types (most abundantly collagen type II) which represent structural and functional transformation during disease advancement. ECM investigation revealed significant biomarkers of OA that could be used as a diagnostic and therapeutic tool in different canine orthopedic diseases. This review deliberates our current findings of how the components of ECM change at the molecular level during disease progression in canine OA.}, language = {en} } @article{GiengRailaRosales2005, author = {Gieng, S. H. and Raila, Jens and Rosales, F. J.}, title = {Accumulation of retinol in the liver after prolonged hyporetinolemia in the vitamin A-sufficient rat}, issn = {0022-2275}, year = {2005}, abstract = {We assessed the effects of prolonged reduction of plasma retinol concentrations (hyporetinolemia) on the distribution of tissue vitamin A (VA) and of its active compounds using a model of continuous recombinant human interleukin-6 (rhIL-6) infusion via osmotic minipumps in VA-sufficient male rats. Plasma retinol and retinol-binding protein (RBP) concentrations remained decreased and lower in rhIL-6- treated rats compared with controls from 7.5 h throughout 7 days of infusion (P < 0.001). This reduction was accompanied by a 68\% increase in hepatic retinol concentration by 7 days (P < 0.05). Hepatic and renal retinyl palmitate and retinoic acid concentrations did not change, and renal megalin content remained unchanged; hepatic RBP concentrations were 41\% lower in rhIL-6-treated rats compared with controls (P < 0.05). These results indicate that instead of being lost, retinol accumulated in the liver during inflammation and that hyporetinolemia was attributable to a decrease in the availability of hepatic RBP. A plausible consequence of the effect of rhIL-6-induced hyporetinolemia is that by 7 days tissues that are dependent on plasma retinol may become deprived of VA. These results have important implications in understanding the mechanism by which measles infection induces hyporetinolemia and VA deficiency of extrahepatic tissues}, language = {en} } @article{SchweigertRailaSehoulietal.2004, author = {Schweigert, Florian J. and Raila, Jens and Sehouli, Jalid and B{\"u}scher, Ulrich}, title = {Accumulation of Selected Carotenoids, alpha-tocopherol and Retinol in Human Ovarian Carcinoma Ascitic Fluid}, issn = {1421-9697}, year = {2004}, abstract = {Background: Patients with severe forms of cancer are reported to have reduced concentrations of micronutrients in plasma due to the chronic reduction of food intake and an increased metabolism of these components. The purpose of this study was to evaluate if an accumulation of carotenoids, alpha-tocopherol and retinol in malignant ascitic fluid in women with ovarian cancer might contribute to a loss of these components from plasma. Methods: Blood and ascitic fluid samples obtained from 21 women with ovarian carcinomas and 17 healthy controls were analyzed for retinol, retinol- binding protein (RBP), alpha-tocopherol and carotenoids. Results: Plasma concentrations of all micronutrients were lower in cancer patients compared to controls. Ascitic fluid concentration of all investigated components was comparable (73- 110\%) to plasma. While the mean concentration of retinol in malignant ascites represented 73\% of that in plasma, the concentration of RBP was less than 10\% resulting in an increased mean molar ratio of retinol to RBP from 1.18 to 10.5. Conclusions: The results suggest that lower plasma concentrations of micronutrients in women suffering from ovarian carcinoma are not only caused by a cachexia-induced decrease of food intake and a higher rate of metabolic utilization, but also by a substantial yet not considered transfer from plasma into ascitic fluid possibly associated with plasma lipoproteins. This raises questions with regard to the protective function of these plasma components in ascitic fluid, the consequences of paracentesis on an additional supplementation and finally the possibility to use one or a combination of these components as an additional marker to discriminate between benign and malignant ascites. Copyright (C) 2004 S. Karger AG, Basel}, language = {en} } @inproceedings{HenzeRailaScholzeetal.2013, author = {Henze, Andrea and Raila, Jens and Scholze, Alexandra and Schweigert, Florian J. and Tepel, Martin}, title = {Administration of N-Acetylcyteine causes beneficial posttranslationalmodifications of transthyretin in hemodialysis patients}, series = {Nephrology, dialysis, transplantation}, volume = {28}, booktitle = {Nephrology, dialysis, transplantation}, number = {2}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {0931-0509}, pages = {164 -- 164}, year = {2013}, language = {en} } @article{HenzeFreyRailaetal.2010, author = {Henze, Andrea and Frey, Simone K. and Raila, Jens and Scholze, Alexandra and Spranger, Joachim and Weickert, Martin O. and Tepel, Martin and Zidek, Walter and Schweigert, Florian J.}, title = {Alterations of retinol-binding protein 4 species in patients with different stages of chronic kidney disease and their relation to lipid parameters}, issn = {0006-291X}, doi = {10.1016/j.bbrc.2010.01.082}, year = {2010}, abstract = {Retinol-binding protein 4 (RBP4) is elevated in patients with chronic kidney disease (CKD) and has been discussed as marker of kidney function. In addition to an elevated concentration, the existence of truncated RBP4 species, RBP4-L (truncated at last C-terminal leucine) and RBP4-LL (truncated at both C-terminal leucines), has been reported in serum of hemodialysis patients. Since little is known about the occurrence of RBP4 species during the progression of CKD it was the aim of this study to analyse this possible association. The presence of RBP4, RBP4-L, RBP4- LL and transthyretin (TTR) was assessed in serum of 45 healthy controls and 52 patients with stage 2-5 of CKD using ELISA and RBP4 immunoprecipitation with subsequent MALDI-TOF-MS analysis. A reduction of glomerular filtration rate was accompanied by a gradual elevation of RBP4 serum levels and relative amounts of RBP4-LL. Correlation analysis revealed a strong association of the RBP4-TTR ratio with parameters of lipid metabolism and with diabetes-related factors. In conclusion, RBP4 serum concentration and the appearance of RBP4-LL seem to be influenced by kidney function. Furthermore, the RBP4-TTR ratio may provide diagnostic potential with regard to metabolic complications in CKD patients.}, language = {en} } @article{SchedlbauerBlaueRailaetal.2020, author = {Schedlbauer, Carola and Blaue, Dominique and Raila, Jens and Vervuert, Ingrid}, title = {Alterations of serum vitamin E and vitamin A concentrations of ponies and horses during experimentally induced obesity}, series = {Journal of animal physiology and animal nutrition}, volume = {104}, journal = {Journal of animal physiology and animal nutrition}, number = {5}, publisher = {Wiley}, address = {Hoboken}, issn = {0931-2439}, doi = {10.1111/jpn.13385}, pages = {1501 -- 1508}, year = {2020}, abstract = {Vitamin A, vitamin E and retinol-binding protein 4 (RBP4) are a focus of current obesity research in humans. The impact of body weight (BW) gain on fat-soluble vitamins and its associated parameters in equines has not been previously reported. Ten Shetland ponies and 9 Warmblood horses, all adult geldings, non-obese and healthy, were fed an excessive energy diet for 20 months to induce BW gain. Serum alpha-tocopherol (vitamin E), retinol (vitamin A), retinol-binding protein 4 (RBP4) and retinol/RBP4 ratio were analysed before BW gain induction and at six timepoints during the BW gaining period. The mean (+/- SD) \% BW gain achieved during two years of excess energy intake was 29.9 +/- 19.4\% for ponies and 17 +/- 6.74\% for horses. Serum alpha-tocopherol increased significantly in ponies and horses during excess energy intake and circulating alpha-tocopherol levels correlated positively with alpha-tocopherol intake (r = .6; p < .001). Serum retinol concentrations showed variations during the study but without relation to intake. Serum RBP4 decreased at the end of the study. The retinol/RBP4 ratio increased with BW gain without differences between ponies and horses. In comparison with human research, the increase in the retinol/RBP4 ratio was unexpected and needs further elucidation.}, language = {en} } @article{ThawnashomTungtrongchitrChanchayetal.2011, author = {Thawnashom, Kittisak and Tungtrongchitr, Rungsunn and Chanchay, Siriporn and Tungtrongchitr, Anchalee and Raila, Jens and Henze, Andrea and Schweigert, Florian J.}, title = {Association between Retinol-Binding protein and renal function among Asian subjects with type 2 diabetes mellitus a cross-sectiona{\"o} study}, series = {The Southeast Asian journal of tropical medicine and public health : official publication of the SEAMEO Regional Tropical Medicine and Public Health Project (TROPMED)}, volume = {42}, journal = {The Southeast Asian journal of tropical medicine and public health : official publication of the SEAMEO Regional Tropical Medicine and Public Health Project (TROPMED)}, number = {4}, publisher = {SEAMEO}, address = {Bangkok}, issn = {0125-1562}, pages = {936 -- 945}, year = {2011}, abstract = {Retinol-binding protein 4 (RBP4) has been suggested as new adipokine, possibly linking obesity to type 2 diabetes mellitus (T2DM). Since the kidneys are the main site of RBP4 degradation and since renal failure is a frequent co-morbid condition with diabetes mellitus, we evaluated the association among RBP4, renal function and T2DM in an Asian population. RBP4 serum levels were analyzed in 110 subjects (50 with T2DM) using an enzyme-linked immunosorbent assay (ELISA). Based on a cut-off estimated glomerular filtration rate (eGFR) of 60 ml/min per 1.73 m(2) (calculated according the abbreviated MDRD formula which uses serum creatinine level, age and gender) and on the T2DM status, subjects were assigned to four subgroups: Group A - controls with an eGFR > 60 ml/min per 1.73 m(2), Group B - controls with an eGFR < 60 ml/min per 1.73 m(2), Group C- T2DM subjects with an eGFR>60 ml/min per 1.73 m(2), and Group D - T2DM subjects with an eGFR <60 ml/ mm per 1.73 m(2). In both the T2DM and control groups, RBP4 levels were higher in subjects with an eGFR < 60 ml/min per 1.73 m(2) than in subjects with an eGFR >60 ml/min per 1.73 m(2). However, the difference was only significant between the control groups (p <0.05). After adjusting for age, gender, BMI, eGFR and the presence of T2DM, eGFR, not T2DM, was associated with plasma RBP4 levels (p<0.05). These results suggest among Asians the eGFR, but not the presence of T2DM, is a major determinant of RBP4 serum levels. The eGFR should be taken into account when evaluating the role of RBP4 in the pathogenesis of insulin resistance and T2DM.}, language = {en} } @article{GriebschArndtRailaetal.2009, author = {Griebsch, Christine and Arndt, Gisela and Raila, Jens and Schweigert, Florian J. and Kohn, Barbara}, title = {C-reactive protein concentration in dogs with primary immune-mediated hemolytic anemia}, issn = {0275-6382}, doi = {10.1111/j.1939-165X.2009.00146.x}, year = {2009}, abstract = {Background Canine primary immune-mediated hemolytic anemia (IMHA) is associated with a high-mortality rate. C- reactive protein (CRP) is the most important acute-phase protein in dogs and may have value as a marker of prognosis or response to treatment in IMHA. Objective The objectives of this study were to evaluate serum CRP concentration in dogs with primary IMHA at presentation and during treatment, to assess potential differences based on survival time, and to compare CRP with other laboratory parameters of inflammation and prognosis. Methods Inclusion criteria for primary IMHA were anemia (PCV < 0.30 L/L), a positive Coombs' test or persistent autoagglutination of erythrocytes, and the exclusion of underlying diseases by other diagnostic tests. Dogs were divided into 2 groups based on survival: dogs that were still alive 14 days after start of treatment (group 1) and dogs that died or were euthanized before day 14 (group 2). Serum CRP concentration, a CBC, and a biochemistry profile were performed on days 0, 3, 8, and 14. Serum CRP also was determined in 25 clinically healthy dogs. Results CRP concentration in the 25 clinically healthy dogs ranged from 0- 8.9 mu g/mL (median 2.2 mu g/mL). Thirty dogs were diagnosed with primary IMHA, 24 in group 1 and 6 in group 2. On day 0, CRP concentration in dogs in both groups (median 224 mu g/mL) was increased above the reference interval. In group 1 dogs, median CRP concentration was 242 mu g/mL on day 0, 69 mu g/mL on day 3, 35 mu g/mL on day 8, and 2 mu g/mL on day 14. In group 2 dogs, median CRP concentration was 194 mu g/mL on day 0, 119 mu g/mL on day 3, and 41 mu g/mL on day 8; only 1 dog in group 2 survived to day 8. There was a significant correlation between CRP and total WBC concentrations on days 0 and 3 (r=-.598, P=.003). Conclusions Serum CRP concentration was markedly increased in dogs with primary IMHA. CRP concentration did not differ based on patient survival, but might be a marker for long-term monitoring of these patients.}, language = {en} } @article{RailaSchweigertKohn2011, author = {Raila, Jens and Schweigert, Florian J. and Kohn, Barbara}, title = {C-reactive protein concentrations in serum of dogs with naturally occurring renal disease}, series = {Journal of veterinary diagnostic investigation}, volume = {23}, journal = {Journal of veterinary diagnostic investigation}, number = {4}, publisher = {Sage Publ.}, address = {Thousand Oaks}, issn = {1040-6387}, doi = {10.1177/1040638711407896}, pages = {710 -- 715}, year = {2011}, abstract = {The current study was undertaken to investigate the relation between serum C-reactive protein (CRP) concentrations and parameters of renal function in dogs with naturally occurring renal disease. Dogs were assigned to groups according to plasma creatinine concentration, urinary protein-to-creatinine ratio (UP/UC), and exogenous plasma creatinine clearance (P-Cl(Cr)) rates. Group A (healthy control dogs; n = 8): non-azotemic (plasma creatinine <125 mu mol/l) and nonproteinuric (UP/UC <0.2), with P-Cl(Cr) rates >90 ml/min/m(2); group B (n = 11): non-azotemic, nonproteinuric dogs with reduced P-Cl(Cr) rates (50-89 ml/min/m(2)); group C (n = 7): azotemic, borderline proteinuric dogs (P-Cl(Cr) rates: 22-67 ml/min/m(2)); and group D (n = 6): uremic, proteinuric dogs (not tested for P-Cl(Cr)). The serum CRP concentrations were measured via commercial enzyme-linked immunosorbent assay. The CRP concentrations in the clinically healthy dogs (group A) ranged from 2.09 mg/l to 8.60 mg/l (median: 3.21 mg/l). In comparison with dogs of group A, median CRP concentrations were significantly (P < 0.01) elevated in dogs of group B (17.6 mg/l, range: 17.0-19.2 mg/l), group C (24.8 mg/l, range: 18.0-32.5 mg/l), and group D (59.7 mg/l, range: 17.7-123 mg/l). Serum CRP was significantly related to P-Cl(Cr) (r = -0.83; P < 0.001), plasma creatinine (r = 0.81; P < 0.001), UP/UC (r = 0.70; P < 0.001), and leukocytes (r = 0.49; P < 0.01). The significant relations between serum CRP concentrations and biochemical parameters of kidney function in plasma and urine suggest that a stimulation of the acute phase response is implicated in the pathogenesis of canine renal disease.}, language = {en} } @article{WeingartRailaLuebkeBeckeretal.2018, author = {Weingart, C. and Raila, Jens and L{\"u}bke-Becker, A. and Kershaw, O. and Brunnberg, M. and Kohn, B.}, title = {Calcitriol induced hypercalcemia in a hunting dog with a disseminated Paecilomyces variotii infection}, series = {Schweizer Archiv f{\"u}r Tierheilkunde}, journal = {Schweizer Archiv f{\"u}r Tierheilkunde}, number = {5}, edition = {160}, publisher = {Gesellschaft Schweizer Tier{\"a}rztinnen und Tier{\"a}rzte}, address = {Bern}, issn = {0036-7281}, doi = {10.17236/sat00161}, pages = {313 -- 319}, year = {2018}, abstract = {A 5-year old hunting dog was presented with reduced appetite, weight loss and polyuria/polydipsia. Hematology and clinical chemistry revealed anemia, leukocytosis, increased liver enzymes, hypoalbuminemia and hypercalcemia. The cytological, pathohistological and microbiological examination identified a disseminated infection with the saprophytic mould fungus Paecilomyces variotii in the biopsies of the spleen and a lymph node. Determination of vitamin D metabolites confirmed a calcitriol induced hypercalcemia.}, language = {en} } @article{ForterreRailaForterreetal.2006, author = {Forterre, Simone and Raila, Jens and Forterre, Franck and Brunnberg, Leo and Schweigert, Florian J.}, title = {Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs}, issn = {1090-0233}, doi = {10.1016/j.tvjl.2004.11.017}, year = {2006}, abstract = {The aim of this study was to investigate differences in concentrations of vitamin A, transthyretin (TTR) and retinol-binding protein (RBP) between plasma and cerebrospinal fluid (CSF) in dogs. RBP was detected using ELISA, and both RBP and TTR by Western blot analysis after separation on SDS-PAGE. Vitamin A was determined by high performance liquid chromatography. RBP and TTR as well as vitamin A were detected in all samples but at substantially lower concentrations in CSF compared to plasma. RBP in dog plasma showed a similar molecular mass to that of humans, whereas canine TTR had a lower molecular mass. Comparison between plasma and CSF showed that both RBP and TTR were of lower molecular mass in CSF. In CSF, RBP and retinol were present at 10-100-fold lower concentrations compared to plasma. Retinyl esters were present only in minute amounts in 5/17 samples. In conclusion, the CSF of dogs compared to humans is significantly different in terms of both quality and quantity of transport proteins for vitamin A.}, language = {en} } @article{ForeterreRailaForterreetal.2004, author = {Foreterre, Simone and Raila, Jens and Forterre, Franck and Brunnberg, Leo and Schweigert, Florian J.}, title = {Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs}, year = {2004}, language = {en} } @article{SchweigertWirthRaila2004, author = {Schweigert, Florian J. and Wirth, Kerstin and Raila, Jens}, title = {Characterization of the microheterogeneity of transthyretin in plasma and urine using SELDI-TOF-MS immunoassay}, year = {2004}, language = {en} } @article{LongardtSchmiedchenRailaetal.2014, author = {Longardt, Ann Carolin and Schmiedchen, B. and Raila, Jens and Schweigert, Florian J. and Obladen, M. and Buehrer, Christoph and Loui, A.}, title = {Characterization of the vitamin A transport in preterm infants after repeated high-dose vitamin A injections}, series = {European journal of clinical nutrition}, volume = {68}, journal = {European journal of clinical nutrition}, number = {12}, publisher = {Nature Publ. Group}, address = {London}, issn = {0954-3007}, doi = {10.1038/ejcn.2014.202}, pages = {1300 -- 1304}, year = {2014}, abstract = {BACKGROUND/OBJECTIVES: Preterm infants have low vitamin A stores at birth, and parenteral administration of high-dose vitamin A reduces pulmonary morbidity. The aim was to characterize vitamin A transport and status. SUBJECTS/METHODS: Prospective study of 69 preterm infants (median birth weight 995 g, gestational age 28 weeks), in which 51 received 5000 IU vitamin A three times per week intramuscular (i.m.) for 4 weeks and 18 infants without i.m. vitamin A served as controls. Serum retinol, retinyl palmitate, total retinol-binding protein 4 (RBP4), retinol-unbound RBP4 (apo-RBP4) and transthyretin concentrations were determined at days 3 (D3) and 28 (D28) of life. RESULTS: D3 retinol concentrations were low for the entire group (382 (285/531) nmol/l; median/interquartile range) and unrelated to gestational age. D28 retinol was unchanged in controls (382 (280/471) nmol/l), but increased in the vitamin A group (596 (480/825) nmol/l; P < 0.001). A similar pattern was observed for RBP4. The calculated retinol-to-RBP4 ratio rose in vitamin A infants (D3: 0.81 (0.57/0.94), D28: 0.98 (0.77/1.26); P < 0.01) but not in controls. In the vitamin A group, the retinol-to-RBP4 ratio was 41 in 15\% of all infants on D3 and in 45\% of infants on D28, but was <= 1 in all, but one, controls on D28. CONCLUSIONS: In preterm infants receiving a 4-week course of high-dose i. m. vitamin A, serum retinol concentrations increased by 55\%, with molar concentrations of retinol exceeding those of RBP4 in 45\% of the infants suggesting transport mechanisms other than RBP4.}, language = {en} } @article{MuellerRailaAltenkampetal.2012, author = {Mueller, K. and Raila, Jens and Altenkamp, Rainer and Schmidt, D. and Dietrich, R. and Hurtienne, Andrea and Wink, M. and Krone, O. and Brunnberg, Leo and Schweigert, Florian J.}, title = {Concentrations of retinol, 3,4-didehydroretinol, and retinyl esters in plasma of free-ranging birds of prey}, series = {Journal of animal physiology and animal nutrition}, volume = {96}, journal = {Journal of animal physiology and animal nutrition}, number = {6}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {0931-2439}, doi = {10.1111/j.1439-0396.2011.01219.x}, pages = {1044 -- 1053}, year = {2012}, abstract = {This study investigated vitamin A compounds in the plasma of healthy free-ranging Central European raptors with different feeding strategies. Plasma samples of nestlings of white-tailed sea eagle [white-tailed sea eagle (WTSE), Haliaeetus albicilla) (n = 32), osprey (Pandion haliaetus) (n = 39), northern goshawk (Accipiter gentilis) (n = 25), common buzzard (Buteo buteo) (n = 31), and honey buzzard (Pernis apivorus) (n = 18) and adults of WTSE (n = 10), osprey (n = 31), and northern goshawk (n = 45) were investigated with reversed-phase-high-performance liquid chromatography (RP-HPLC). In WTSE, northern goshawks and common buzzards retinol were the main plasma component of vitamin A, whilst in ospreys and honey buzzards, 3,4-didehydroretinol predominated. The median of the retinol plasma concentration in the nestlings group ranged from 0.12 to 3.80 mu M and in the adult group from 0.15 to 6.13 mu M. Median plasma concentrations of 3,4-didehydroretinol in nestlings ranged from 0.06 to 3.55 mu M. In adults, northern goshawks had the lowest plasma concentration of 3,4-didehydroretinol followed by WTSE and ospreys. The plasma of all investigated species contained retinyl esters (palmitate, oleate, and stearate). The results show considerable species-specific differences in the vitamin A plasma concentrations that might be caused by different nutrition strategies.}, language = {en} } @inproceedings{KhalilIsalmRailaetal.2011, author = {Khalil, Mahomound and Isalm, K. Shaiful and Raila, Jens and Schenk, R. and Rawel, Harshadrai Manilal and Schweigert, Florian J.}, title = {Content of lutein and lutein ester in tagetes and improvement of their stability}, series = {Annals of nutrition \& metabolism : journal of nutrition, metabolic diseases and dietetics ; an official journal of International Union of Nutritional Sciences (IUNS)}, volume = {58}, booktitle = {Annals of nutrition \& metabolism : journal of nutrition, metabolic diseases and dietetics ; an official journal of International Union of Nutritional Sciences (IUNS)}, number = {3}, publisher = {Karger}, address = {Basel}, issn = {0250-6807}, pages = {16 -- 16}, year = {2011}, language = {en} } @article{AuyyuenyongHenzeUngruetal.2017, author = {Auyyuenyong, Ratchada and Henze, Andrea and Ungru, Julia and Schweigert, Florian Johannes and Raila, Jens and Vervuert, Ingrid}, title = {Determination of lipid profiles in serum of obese ponies before and after weight reduction by using multi-one-dimensional thin-layer chromatography}, series = {Research in veterinary science}, volume = {117}, journal = {Research in veterinary science}, publisher = {Elsevier}, address = {Oxford}, issn = {0034-5288}, doi = {10.1016/j.rvsc.2017.11.013}, pages = {111 -- 117}, year = {2017}, abstract = {Obesity is a key component of equine metabolic syndrome, which is highly associated with laminitis. Feed restriction and/or exercise are known to alleviate the detrimental effects of insulin resistance in obese ponies. However, little is known about changes in the serum lipid patterns due to weight reduction and its association with disease outcomes. Therefore, the lipid patterns in the serum of 14 mature ponies before and after a 14-week body weight reduction program (BWRP) were investigated by multi-one-dimensional thin-layer chromatography (MOD-TLC). Additionally, sensitivity to insulin (SI), body condition scores (BCS) and cresty neck scores (CNS) were measured. A BWRP resulted in a significant loss of body weight (P < 0.001), which was associated with beneficial decreases in BCS and CNS (both, P < 0.001). Serum lipid compositions revealed significantly increased free fatty acid (FFA), sphingomyelin (SM; both P < 0.001), total cholesterol (C) and cholesterol ester (CE) (both P < 0.01) and triacylglycerol (TG; P < 0.05) densities. Improvement of SI after the BWRP was associated with increases in neutral lipids (C, CE and TG, all P < 0.01), FFA and the phospholipid SM (both, P < 0.001). The results show that a BWRP in obese ponies was effective and associated with changes in the concentrations of neutral lipids and the phospholipid SM, indicating that SM may play a role in insulin signaling pathways and thus in the pathogenesis of insulin resistance and the progression of metabolic syndrome in obese ponies.}, language = {en} } @article{RailaForterreSchweigert2003, author = {Raila, Jens and Forterre, Simone and Schweigert, Florian J.}, title = {Die Bedeutung von Megalin in der Pathogenese der tubul{\"a}ren Proteinurie des Hundes}, isbn = {3-936815-65-8}, year = {2003}, language = {de} } @article{SchweigertRailaEisenachetal.1997, author = {Schweigert, Florian J. and Raila, Jens and Eisenach, C. and Buchholz, Ingeborg}, title = {Distribution of vitamin A and retinol-binding-protein (RBP) in plasma, urine and various tissues of canines}, year = {1997}, language = {en} } @article{RailaEisenachBuchholzetal.1997, author = {Raila, Jens and Eisenach, C. and Buchholz, Ingeborg and Schweigert, Florian J.}, title = {Distribution of vitamin A and retinol-binding-protein (RBP) in various tissue of dogs and reccoon dogs}, year = {1997}, language = {en} }