@article{KurokiTchoupaHartliebetal.2019,
  author    = {Kuroki, Agnes and Tchoupa, Arnaud Kengmo and Hartlieb, Matthias and Peltier, Raoul and Locock, Katherine E. S. and Unnikrishnan, Meera and Perrier, Sebastien},
  title     = {Targeting intracellular, multi-drug resistant Staphylococcus aureus with guanidinium polymers by elucidating the structure-activity relationship},
  series = {Biomaterials : biomaterials reviews online},
  volume    = {217},
  journal   = {Biomaterials : biomaterials reviews online},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {0142-9612},
  doi       = {10.1016/j.biomaterials.2019.119249},
  pages     = {13},
  year      = {2019},
  abstract  = {Intracellular persistence of bacteria represents a clinical challenge as bacteria can thrive in an environment protected from antibiotics and immune responses. Novel targeting strategies are critical in tackling antibiotic resistant infections. Synthetic antimicrobial peptides (SAMPs) are interesting candidates as they exhibit a very high antimicrobial activity. We first compared the activity of a library of ammonium and guanidinium polymers with different sequences (statistical, tetrablock and diblock) synthesized by RAFT polymerization against methicillin-resistant S. aureus (MRSA) and methicillin-sensitive strains (MSSA). As the guanidinium SAMPs were the most potent, they were used to treat intracellular S. aureus in keratinocytes. The diblock structure was the most active, reducing the amount of intracellular MSSA and MRSA by two-fold. We present here a potential treatment for intracellular, multi-drug resistant bacteria, using a simple and scalable strategy.},
  language  = {en}
}
@article{HartliebCatrouilletKurokietal.2019,
  author    = {Hartlieb, Matthias and Catrouillet, Sylvain and Kuroki, Agnes and Sanchez-Cano, Carlos and Peltier, Raoul and Perrier, Sebastien},
  title     = {Stimuli-responsive membrane activity of cyclic-peptide-polymer conjugates},
  series = {Chemical science},
  volume    = {10},
  journal   = {Chemical science},
  number    = {21},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  issn      = {2041-6520},
  doi       = {10.1039/c9sc00756c},
  pages     = {5476 -- 5483},
  year      = {2019},
  abstract  = {Cyclic peptide nanotubes (CPNT) consisting of an even number of amino acids with an alternating chirality are highly interesting materials in a biomedical context due to their ability to insert themselves into cellular membranes. However, unwanted unspecific interactions between CPNT and non-targeted cell membranes are a major drawback. To solve this issue we have synthetized a series of CPNT-polymer conjugates with a cleavable covalent connection between macromolecule and peptide. As a result, the polymers form a stabilizing and shielding shell around the nanotube that can be cleaved on demand to generate membrane active CPNT from non-active conjugates. This approach enables us to control the stacking and lateral aggregation of these materials, thus leading to stimuli responsive membrane activity. Moreover, upon activation, the systems can be adjusted to form nanotubes with an increased length instead of aggregates. We were able to study the dynamics of these systems in detail and prove the concept of stimuli responsive membrane interaction using CPNT-polymer conjugates to permeabilize liposomes as well as mammalian cell membranes.},
  language  = {en}
}
@article{FloydSongHapeshietal.2022,
  author    = {Floyd, Thomas G. and Song, Ji-Inn and Hapeshi, Alexia and Laroque, Sophie and Hartlieb, Matthias and Perrier, Sebastien},
  title     = {Bottlebrush copolymers for gene delivery: influence of architecture, charge density, and backbone length on transfection efficiency},
  series = {Journal of materials chemistry : B, materials for biology and medicine},
  volume    = {10},
  journal   = {Journal of materials chemistry : B, materials for biology and medicine},
  number    = {19},
  publisher = {Royal Society of Chemistry},
  address   = {London [u.a.]},
  issn      = {2050-750X},
  doi       = {10.1039/d2tb00490a},
  pages     = {3696 -- 3704},
  year      = {2022},
  abstract  = {The influence of polymer architecture of polycations on their ability to transfect mammalian cells is probed. Polymer bottle brushes with grafts made from partially hydrolysed poly(2-ethyl-2-oxazoline) are used while varying the length of the polymer backbone as well as the degree of hydrolysis (cationic charge content). Polyplex formation is investigated via gel electrophoresis, dye-displacement and dynamic light scattering. Bottle brushes show a superior ability to complex pDNA when compared to linear copolymers. Also, nucleic acid release was found to be improved by a graft architecture. Polyplexes based on bottle brush copolymers showed an elongated shape in transmission electron microscopy images. The cytotoxicity against mammalian cells is drastically reduced when a graft architecture is used instead of linear copolymers. Moreover, the best-performing bottle brush copolymer showed a transfection ability comparable with that of linear poly(ethylenimine), the gold standard of polymeric transfection agents, which is used as positive control. In combination with their markedly lowered cytotoxicity, cationic bottle brush copolymers are therefore shown to be a highly promising class of gene delivery vectors.},
  language  = {en}
}
@article{HartliebMansfieldPerrier2020,
  author    = {Hartlieb, Matthias and Mansfield, Edward D. H. and Perrier, Sebastien},
  title     = {A guide to supramolecular polymerizations},
  series = {Polymer Chemistry},
  volume    = {11},
  journal   = {Polymer Chemistry},
  number    = {6},
  publisher = {Royal Society of Chemistry},
  address   = {Cambridge},
  issn      = {1759-9954},
  doi       = {10.1039/c9py01342c},
  pages     = {1083 -- 1110},
  year      = {2020},
  abstract  = {Supramolecular polymers or fibers are non-covalent assemblies of unimeric building blocks connected by secondary interactions such as hydrogen bonds or pi-pi interactions. Such structures hold enormous potential in the development of future materials, as their non-covalent nature makes them highly modular and adaptive. Within this review we aim to provide a broad overview over the area of linear supramolecular polymers including the different mechanisms of their polymerization as well as methods essential for their characterization. The different non-covalent interactions able to form supramolecular polymers are discussed, and key examples for each species are shown. Particular emphasis is laid on the development of living supramolecular polymerization able to produce fibers with a controlled length and low length dispersity, and even enable the production of supramolecular block copolymers. Another important and very recent field is the development of out-of-equilibrium supramolecular polymers, where the polymerization process can be temporally controlled enabling access to highly adaptive materials.},
  language  = {en}
}