@article{SprengerRudackSchudomaetal.2015,
  author    = {Sprenger, Heike and Rudack, Katharina and Schudoma, Christian and Neumann, Arne and Seddig, Sylvia and Peters, Rolf and Zuther, Ellen and Kopka, Joachim and Hincha, Dirk K. and Walther, Dirk and Koehl, Karin},
  title     = {Assessment of drought tolerance and its potential yield penalty in potato},
  series = {Functional plant biology : an international journal of plant function},
  volume    = {42},
  journal   = {Functional plant biology : an international journal of plant function},
  number    = {7},
  publisher = {CSIRO},
  address   = {Clayton},
  issn      = {1445-4408},
  doi       = {10.1071/FP15013},
  pages     = {655 -- 667},
  year      = {2015},
  abstract  = {Climate models predict an increased likelihood of seasonal droughts for many areas of the world. Breeding for drought tolerance could be accelerated by marker-assisted selection. As a basis for marker identification, we studied the genetic variance, predictability of field performance and potential costs of tolerance in potato (Solanum tuberosum L.). Potato produces high calories per unit of water invested, but is drought-sensitive. In 14 independent pot or field trials, 34 potato cultivars were grown under optimal and reduced water supply to determine starch yield. In an artificial dataset, we tested several stress indices for their power to distinguish tolerant and sensitive genotypes independent of their yield potential. We identified the deviation of relative starch yield from the experimental median (DRYM) as the most efficient index. DRYM corresponded qualitatively to the partial least square model-based metric of drought stress tolerance in a stress effect model. The DRYM identified significant tolerance variation in the European potato cultivar population to allow tolerance breeding and marker identification. Tolerance results from pot trials correlated with those from field trials but predicted field performance worse than field growth parameters. Drought tolerance correlated negatively with yield under optimal conditions in the field. The distribution of yield data versus DRYM indicated that tolerance can be combined with average yield potentials, thus circumventing potential yield penalties in tolerance breeding.},
  language  = {en}
}
@article{ThalhammerHundertmarkPopovaetal.2010,
  author    = {Thalhammer, Anja and Hundertmark, Michaela and Popova, Antoaneta V. and Seckler, Robert and Hincha, Dirk K.},
  title     = {Interaction of two intrinsically disordered plant stress proteins (COR15A and COR15B) with lipid membranes in the dry state},
  issn      = {0005-2736},
  doi       = {10.1016/j.bbamem.2010.05.015},
  year      = {2010},
  abstract  = {COR15A and COR15B form a tandem repeat of highly homologous genes in Arabidopsis thaliana. Both genes are highly cold induced and the encoded proteins belong to the Pfam LEA_4 group (group 3) of the late embryogenesis abundant (LEA) proteins. Both proteins were predicted to be intrinsically disordered in solution. Only COR15A has previously been characterized and it was shown to be localized in the soluble stroma fraction of chloroplasts. Ectopic expression of COR15A in Arabidopsis resulted in increased freezing tolerance of both chloroplasts after freezing and thawing of intact leaves and of isolated protoplasts frozen and thawed in vitro. In the present study we have generated recombinant mature COR15A and COR15B for a comparative study of their structure and possible function as membrane protectants. CD spectroscopy showed that both proteins are predominantly unstructured in solution and mainly a-helical after drying. Both proteins showed similar effects on the thermotropic phase behavior of dry liposomes. A decrease in the gel to liquid-crystalline phase transition temperature depended on both the unsaturation of the fatty acyl chains and lipid headgroup structure. FTIR spectroscopy indicated no strong interactions between the proteins and the lipid phosphate and carbonyl groups, but significant interactions with the galactose headgroup of the chloroplast lipid monogalactosyldiacylglycerol. These findings were rationalized by modeling the secondary structure of COR15A and COR15B. Helical wheel projection indicated the presence of amphipathic a-helices in both proteins. The helices lacked a clear separation of positive and negative charges on the hydrophilic face, but contained several hydroxylated amino acids.},
  language  = {en}
}