@article{RudolphMohrGottfriedLamshoeftetal.2015,
  author    = {Rudolph-Mohr, Nicole and Gottfried, Sebastian and Lamsh{\"o}ft, Marc and Z{\"u}hlke, Sebastian and Oswald, Sascha and Spiteller, Michael},
  title     = {Non-invasive imaging techniques to study O-2 micro-patterns around pesticide treated lupine roots},
  series = {Geoderma : an international journal of soil science},
  volume    = {239},
  journal   = {Geoderma : an international journal of soil science},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0016-7061},
  doi       = {10.1016/j.geoderma.2014.10.022},
  pages     = {257 -- 264},
  year      = {2015},
  abstract  = {The soil root interface is a highly heterogeneous system, e.g. in terms of O-2 and pH distribution. The destructive character of conventional methods disturbs the natural conditions of those biogeochemical gradients. Therefore, experiments aiming to control these influences and study pesticide kinetics under given O-2 and pH conditions suffer from a large uncertainty of the "real" O-2/pH at a certain position. Our approach with two different imaging techniques will examine the soil-root interface as well as the dissipation of the applied pesticide at a high spatial resolution. The obtained outcomes show directly that the pH has an influence on enantioselective dissipation of the acetanilide fungicide metalaxyl. In areas with high pH from an applied racemic mixture, the R-enantiomer dissipates faster than the S-enantiomer. Moreover, we found significantly reduced oxygen values in the bulk soil and vicinity of metalaxyl treated roots compared to control plant roots. The combination of matrix-assisted laser desorption/ionization mass spectrometry (MALDI) and fluorescence imaging indicated the oxygen-dependent behavior of metalaxyl at the root surface. The results presented here underline the great potential of combining different imaging methods to examine the soil-root interfaces as well as the dissipation of organic pollutants in small soil compartments. (C) 2014 Elsevier B.V. All rights reserved.},
  language  = {en}
}
@article{JahnBuschmannHille2015,
  author    = {Jahn, Karolina and Buschmann, Volker and Hille, Carsten},
  title     = {Simultaneous Fluorescence and Phosphorescence Lifetime Imaging Microscopy in Living Cells},
  series = {Scientific Reports},
  journal   = {Scientific Reports},
  number    = {5},
  publisher = {Nature Publishing Group},
  address   = {London},
  issn      = {2045-2322},
  doi       = {10.1038/srep14334},
  pages     = {13},
  year      = {2015},
  abstract  = {In living cells, there are always a plethora of processes taking place at the same time. Their precise regulation is the basis of cellular functions, since small failures can lead to severe dysfunctions. For a comprehensive understanding of intracellular homeostasis, simultaneous multiparameter detection is a versatile tool for revealing the spatial and temporal interactions of intracellular parameters. Here, a recently developed time-correlated single-photon counting (TCSPC) board was evaluated for simultaneous fluorescence and phosphorescence lifetime imaging microscopy (FLIM/PLIM). Therefore, the metabolic activity in insect salivary glands was investigated by recording ns-decaying intrinsic cellular fluorescence, mainly related to oxidized flavin adenine dinucleotide (FAD) and the μs-decaying phosphorescence of the oxygen-sensitive ruthenium-complex Kr341. Due to dopamine stimulation, the metabolic activity of salivary glands increased, causing a higher pericellular oxygen consumption and a resulting increase in Kr341 phosphorescence decay time. Furthermore, FAD fluorescence decay time decreased, presumably due to protein binding, thus inducing a quenching of FAD fluorescence decay time. Through application of the metabolic drugs antimycin and FCCP, the recorded signals could be assigned to a mitochondrial origin. The dopamine-induced changes could be observed in sequential FLIM and PLIM recordings, as well as in simultaneous FLIM/PLIM recordings using an intermediate TCSPC timing resolution.},
  language  = {en}
}