@inproceedings{HolzloehnerSchliebsMaieretal.2013,
  author    = {Holzl{\"o}hner, Pamela and Schliebs, Erik and Maier, Natalia and F{\"u}ner, Jonas and Micheel, Burkhard and Heilmann, Katja},
  title     = {Production of monoclonal camelid antibodies by means of hybridoma technology},
  series = {The journal of immunology},
  volume    = {190},
  booktitle = {The journal of immunology},
  publisher = {American Assoc. of Immunologists},
  address   = {Bethesda},
  issn      = {0022-1767},
  pages     = {1},
  year      = {2013},
  language  = {en}
}
@article{KabaMaierSchliebeOhleretal.2015,
  author    = {Kaba, Hani E. J. and Maier, Natalia and Schliebe-Ohler, Nicole and Mayer, Yvonne and Mueller, Peter P. and van den Heuvel, Joop and Schuchhardt, Johannes and Hanack, Katja and Bilitewski, Ursula},
  title     = {Identification of whole pathogenic cells by monoclonal antibodies generated against a specific peptide from an immunogenic cell wall protein},
  series = {Journal of microbiological methods},
  volume    = {108},
  journal   = {Journal of microbiological methods},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0167-7012},
  doi       = {10.1016/j.mimet.2014.11.003},
  pages     = {61 -- 69},
  year      = {2015},
  abstract  = {We selected the immunogenic cell wall beta-(1,3)-glucosyltransferase Bgl2p from Candida albicans as a target protein for the production of antibodies. We identified a unique peptide sequence in the protein and generated monoclonal anti- C. albicans Bgl2p antibodies, which bound in particular to whole C. albicans cells.},
  language  = {en}
}
@article{HolzloehnerButzeMaieretal.2018,
  author    = {Holzl{\"o}hner, Pamela and Butze, Monique and Maier, Natalia and Hebel, Nicole and Schliebs, Erik and Micheel, Burkhard and Fuener, Jonas and Heidicke, Gabriele and Hanack, Katja},
  title     = {Generation of murine monoclonal antibodies with specificity against conventional camelid IgG1 and heavy-chain only IgG2/3},
  series = {Veterinary Immunology and Immunopathology},
  volume    = {197},
  journal   = {Veterinary Immunology and Immunopathology},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0165-2427},
  doi       = {10.1016/j.vetimm.2018.01.006},
  pages     = {1 -- 6},
  year      = {2018},
  abstract  = {Camelids possess antibodies with a conventional four-chain structure consisting of two heavy and two light chains (of subclass IgG1) but further they also generate heavy-chain only antibodies (of subclass IgG2 and 3) which are fully functional in antigen binding. In this study subclass-specific murine monoclonal antibodies specific to conventional camelid IgG1 and heavy-chain only IgG2/3 were generated and validated for the use as potent secondary detection reagents. The monoclonal antibodies are able to differentiate between all camelid IgGs, conventional four-chain camelid antibodies (of subclass IgG1) and exclusively heavy chain-only antibodies (of subclasses IgG2 and IgG3). Further these antibodies were used to detect specific immune responses after vaccination of Camelids against bovine corona- and rotavirus strains and different E.coli. and Clostridia - antigens and to identify Erysipelothrix rhusiopathiae infected animals within a herd. The described antibodies are suitable as new secondary agents for the detection of different camelid subclasses and the validation of camelid immune reactions.},
  language  = {en}
}
@misc{MaierHolzloehnerHoenowetal.2016,
  author    = {Maier, Natalia and Holzl{\"o}hner, Pamela and Hoenow, Anja and Scheunemann, Astrid and Weschke, Daniel and Hanack, Katja},
  title     = {Characterization of monoclonal antibodies generated by in vitro immunization},
  series = {The journal of immunology},
  volume    = {196},
  journal   = {The journal of immunology},
  publisher = {American Assoc. of Immunologists},
  address   = {Bethesda},
  issn      = {0022-1767},
  pages     = {2},
  year      = {2016},
  abstract  = {Monoclonal antibodies are highly valuable tools in biomedicine but the generation by hybridoma technology is very time-consuming and elaborate. In order to circumvent the consisting drawbacks an in vitro immunization approach was established by which murine as well as human monoclonal antibodies against a viral coat protein could be developed. The in vitro immunization process was performed by isolation of murine hematopoietic stem cells or human monocytes and an in vitro differentiation into immature dendritic cells. After antigen loading the cells were co-cultivated with naive T and B lymphocytes for three days in order to obtain antigen-specific B lymphocytes in culture, followed by fusion with murine myeloma cells or human/murine heteromyeloma cells. Antigen-specific hybridomas were selected and the generated antibodies were purified and characterized in this study by ELISA, western blot, gene sequencing, affinity measurements. Further the characteristics were compared to a monoclonal antibody against the same target generated by conventional hybridoma technology. Isotype detection revealed a murine IgM and a human IgG4 antibody in comparison to an IgG1 for the conventionally generated antibody. The antibodies derived from in vitro immunization showed indeed a lower affinity for the antigen as compared to the conventionally generated one, which is probably based on the significantly shorter B cell maturation (3 days) during the immunization process. Nevertheless, they were suitable for building up a sandwich based detection system. Therefore, the in vitro immunization approach seems to be a good and particularly fast alternative to conventional hybridoma technology.},
  language  = {en}
}
@phdthesis{Maier2016,
  author    = {Maier, Natalia},
  title     = {Aufbau eines Testsystems zum Nachweis von Ethylglucuronid (EtG) in Haaren},
  school      = {Universit{\"a}t Potsdam},
  pages     = {IX, 122},
  year      = {2016},
  language  = {de}
}