@article{Walz1997, author = {Walz, Bernd}, title = {{\"U}berlebensk{\"u}nstler aus dem Moospolster}, year = {1997}, abstract = {Popul{\"a}rwissenschaftlicher Aufsat}, language = {de} } @book{Wartenberg2006, author = {Wartenberg, Maria}, title = {{\"U}ber Nutzen und Grenzen der Stammzellenforschung : Antrittsvorlesung 2006-07-06}, publisher = {Univ.-Bibl.}, address = {Potsdam}, year = {2006}, abstract = {Die Referentin erl{\"a}utert den Ursprung embryonaler Stammzellen und woher sie kommen. Gezeigt wird weiter unter anderem die Gewinnung embryonaler Stammzellen aus der Blastozyste der Maus, und es wird auf die Differenzierung von glatter Muskulatur und Knochen eingegangen. Die Wissenschaftlerin sieht in der Etablierung neuer in vitro Modelle eine große Perspektive f{\"u}r die Forschung mit Maus-Stammzellen.}, language = {de} } @phdthesis{Schorling2005, author = {Schorling, Markus}, title = {{\"O}kologische und phytomedizinische Untersuchungen zum Anbau von Bt-Mais im Maisz{\"u}nsler-Befallsgebiet Oderbruch}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-6260}, school = {Universit{\"a}t Potsdam}, year = {2005}, abstract = {In den letzten 20 Jahren hat sich der Maisz{\"u}nsler (Ostrinia nubilalis H{\"U}BNER), aus der Schmetterlingsfamilie der Pyralidae oder Z{\"u}nsler, zum bedeutendsten tierischen Sch{\"a}dling des Maises (Zea mays) entwickelt. Eine M{\"o}glichkeit den Befall des Maisz{\"u}nslers abzuwenden, bietet der Anbau von Bacillus thuringiensis-Mais (Bt-Mais). Mit Hilfe der Gentechnik wurden Gene des Bakteriums Bacillus thuringiensis {\"u}bertragen, die einen f{\"u}r Fraßinsekten giftigen Wirkstoff bilden, wodurch die Pflanzen w{\"a}hrend der kompletten Vegetation vor den Larven des Maisz{\"u}nslers gesch{\"u}tzt sind. Ziel des vorliegenden Projektes war es, in einer 3-j{\"a}hrigen Studie die Auswirkungen des großfl{\"a}chigen Anbaus von Bt-Mais auf die {\"o}kologische Situation und den Handlungsrahmen des integrierten Pflanzenschutzes komplex zu untersuchen. Dazu wurden in Betrieben im Oderbruch, das als permanentes Befallsgebiet des Maisz{\"u}nslers gilt, in den Jahren 2002 bis 2004 j{\"a}hrlich zwei Felder mit jeweils einer Bt-Sorte und einer konventionellen Sorte angelegt. Zus{\"a}tzlich wurden biologische und chemische Maisz{\"u}nsler-Bek{\"a}mpfungsvarianten gepr{\"u}ft. Durch verschiedene Methoden wie Bonituren, Ganzpflanzenernten, Bodenfallenf{\"a}nge und Beobachtungen des Wahlverhaltens von (Flug-)insekten konnten Aussagen zum Vorkommen von Insekten und Spinnentieren getroffen werden, wobei hierf{\"u}r Daten aus Untersuchungen der Jahre 2000 und 2001 im Oderbruch erg{\"a}nzend herangezogen werden konnten. Durch Ertragsmessungen, Energie- und Qualit{\"a}tsermittlungen, sowie Fusarium- und Mykotoxinanalysen konnte der Anbau von Bt-Mais als neue Alternative zur Bek{\"a}mpfung des Maisz{\"u}nslers bewertet werden. Bez{\"u}glich des Auftretens von Insekten und Spinnentieren wurden im Mittel der f{\"u}nfj{\"a}hrigen Datenerhebung beim Vergleich der Bt-Sorte zur konventionellen Sorte, mit Ausnahme der fast 100 \%igen Bek{\"a}mpfung des Maisz{\"u}nslers, keine signifikanten Unterschiede festgestellt. Hierf{\"u}r wurde ein besonderes Augenmerk auf Thripse, Wanzen, Blattl{\"a}use und deren Fraßfeinde, sowie mittels Bodenfallenf{\"a}ngen auf Laufk{\"a}fer und Spinnen gerichtet. Die erwarteten {\"o}konomischen Vorteile wie etwa Ertragsplus oder bessere N{\"a}hrstoff- und Energiegehalte durch geringeren Schaden beim Anbau von Bt-Mais als Silomais blieben in den Untersuchungsjahren aus. Allerdings zeigten Fusarium- und Mykotoxinanalysen eine geringere Belastung des Bt-Maises, was m{\"o}glicherweise auf den geringeren Schaden zur{\"u}ckzuf{\"u}hren ist, da besch{\"a}digte Pflanzen f{\"u}r Fusarium und Mykotoxine anf{\"a}lliger sind. Desweiteren konnten erste methodische Ans{\"a}tze f{\"u}r ein auf EU-Ebene gefordertes, den Anbau von Bt-Mais begleitendes Monitoring, erarbeitet werden. So konnten Vorschl{\"a}ge f{\"u}r geeignete Methoden, deren Umfang sowie des Zeitpunktes der Durchf{\"u}hrungen gemacht werden.}, subject = {Maisz{\"u}nsler}, language = {de} } @article{Jeltsch2003, author = {Jeltsch, Florian}, title = {{\"O}kologische Forschungen an der Unteren Havel}, series = {Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakult{\"a}t der Universit{\"a}t Potsdam}, volume = {13}, journal = {Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakult{\"a}t der Universit{\"a}t Potsdam}, issn = {1434-2375}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-4100}, pages = {138 -- 139}, year = {2003}, language = {de} } @article{WallschlaegerBeierBurkartetal.2004, author = {Wallschl{\"a}ger, Hans-Dieter and Beier, Wolfgang and Burkart, Michael and Mrzljak, Jadranka and Oehlschl{\"a}ger, Susanne and Wanner, Manfred}, title = {{\"O}kologische Datenerfassung f{\"u}r Naturschutzbewertung und Monitoring im Offenland}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{BeierHinrichsenKlatt1997, author = {Beier, Wolfgang and Hinrichsen, A. and Klatt, Reimund}, title = {{\"O}kofaunistische Untersuchungen auf ausgew{\"a}hlten Truppen{\"u}bungspl{\"a}tzen Brandenburgs}, series = {Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakult{\"a}t der Universit{\"a}t Potsdam}, volume = {1}, journal = {Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakult{\"a}t der Universit{\"a}t Potsdam}, issn = {1434-2375}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-2788}, pages = {130 -- 137}, year = {1997}, language = {de} } @article{Kummer2010, author = {Kummer, Volker}, title = {Zusammenstellung lokaler Pilzliteratur 2004 - 2008}, issn = {0232-4598}, year = {2010}, language = {de} } @article{Kummer2005, author = {Kummer, Volker}, title = {Zusammenstellung lokaler Pilzliteratur 2000 - 2003}, year = {2005}, language = {de} } @article{Kummer1996, author = {Kummer, Volker}, title = {Zur Wasserpflanzenvegetation des Neuendorfer Sees}, year = {1996}, language = {de} } @article{FischerKummer1994, author = {Fischer, Wolfgang and Kummer, Volker}, title = {Zur Verbreitung und Soziologie von S colochloa festucacea L : in Nordostdeutschland und seine Unterscheidung von {\"a}hnlichen Gr{\"a}sern nach vegetativen Merkmalen}, year = {1994}, language = {de} } @article{SeitzRistowKlemmetal.2004, author = {Seitz, Birgit and Ristow, Michael and Klemm, Gunther and R{\"a}tzel, Stefan and Schulze, Gerhart and Hoffmann, Maik}, title = {Zur Verbreitung der Wildrosen und verwilderten Kulturrosen in Berlin und Brandenburg}, issn = {0724-3111 -}, year = {2004}, language = {de} } @article{FischerKummerPoetsch1994, author = {Fischer, Wolfgang and Kummer, Volker and P{\"o}tsch, Joachim}, title = {Zur Vegetation des Feuchtgebietes Internationaler Bedeutung (FIB) Untere Havel}, year = {1994}, language = {de} } @article{KnoescheBukowsky1996, author = {Kn{\"o}sche, R{\"u}diger and Bukowsky, Heinz}, title = {Zur Schwermetallakkumulation in Dactylis glomerata auf Rieselfeldb{\"o}den}, isbn = {3-7983-1680-5}, year = {1996}, language = {de} } @article{Kummer1996, author = {Kummer, Volker}, title = {Zur Pilzflora des Kienbergs bei Wolsier mit Hinweisen auf die Phanerogamenflora}, year = {1996}, language = {de} } @article{Knoesche2003, author = {Kn{\"o}sche, Ralf}, title = {Zur Phospatr{\"u}ckl{\"o}sung aus Augew{\"a}ssersedimenten unter ver{\"a}nderter Hochwasserdynamik}, series = {Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakult{\"a}t der Universit{\"a}t Potsdam}, volume = {13}, journal = {Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakult{\"a}t der Universit{\"a}t Potsdam}, issn = {1434-2375}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-4077}, pages = {102 -- 113}, year = {2003}, language = {de} } @article{BaumannEckermannMeinel1994, author = {Baumann, Guido and Eckermann, Nora and Meinel, Thomas}, title = {Zur Kohlenstoffassimilation in gr{\"u}nen Zuckerr{\"u}ben-Kalluskulturen}, year = {1994}, language = {de} } @article{RistowSeitz2002, author = {Ristow, Michael and Seitz, Birgit}, title = {Zur Kenntnis einiger {\"u}bersehener, wenig beachteter oder verkannter Sippen der Gattungen Vicia und Valerianella in Brandenburg}, year = {2002}, language = {de} } @phdthesis{Oehlschlaeger2001, author = {Oehlschl{\"a}ger, Susanne}, title = {Zur Habitatwahl, Nahrungs{\"o}kologie und Brutbiologie des Wiederhopfes (Upupa epops) Linn{\´e} 1958 auf den ehemaliegen Truppen{\"u}bungspl{\"a}tzen bei J{\"u}terbog, Brandenburg}, pages = {70, XXIII S.}, year = {2001}, language = {de} } @article{Scheffler2008, author = {Scheffler, Ingo}, title = {Zur F{\"a}higkeit von Ektoparasiten der Flederm{\"a}use ihre Wirte aktiv aufzusuchen}, year = {2008}, abstract = {Active host-searching in bat ectoparasites. As a pilot study, this paper discribes the mobility of ectoparasites after removing from their hosts. Spinturnix acuminatus (Koch, 1836)(Acari, Spinturnicidae) a permanent stationary bat mite normally survives the removing only a few hours. This species moves easily on the patagium or on human skin. In contrast, on other surfaces the movements of this species seems to be less orientated and it is unlikely that the mite can reach a host in some distance. Nycteribia kolenatii Theodor \& Moscona, 1954 (Diptera: Nycteribiidae) a bat fly, shows excellent movements in the fur of its host. The specimens survive the removing from the host several days. The ability to clamber up a wall was tested with a rough clay brick. The bat fly achieves only 9,6 cm on average. This result does not support good abilities for host searching in this species. Bat fleas (Siphonaptera: Ischnopsyllidae): Ischnopsyllus elongatus (Curtis, 1832), I. hexactenus (Kolenati, 1856), I. simplex (Rothschild, 1906)and other species survive the removing from their hosts several days. In case of a vertical barrier they showed a stereotype upside climbing, followed by a horizontal appetitive behavior. The fleas were climbing a distance in the upside of 6,45 m on average(1,2-21,68 m). As excellent climbers bat fleas are able to overcome edges and slopes and to crawl on the ceiling. Therefore, it seems to be no problem for a flea to parasite any bat in a resting room. Additionally, some bugs (Cimex lectularius Linnaeus, 1758) were examinated. They run with a speed of 63 cm per minute and were able to climb easily on the brick. On average their stretch of way achieved 2,85 m in ten minutes.}, language = {de} } @article{GlaeserSchoenfelder1994, author = {Gl{\"a}ser, Hans-Joachim and Sch{\"o}nfelder, J.}, title = {Zur Faunistik, Biologie und {\"O}kologie der Wasserfl{\"o}he (Crustacea, Cladocera) der Havelaue - ein Beitrag f{\"u}r einen {\"o}kosystemaren Restaurationsansatz}, year = {1994}, language = {de} } @article{Bergstedt1999, author = {Bergstedt, Christel}, title = {Zur Erarbeitung von Aufgabenstellungen im Fach Biologie auf der Grundlage des Rahmenplans}, year = {1999}, language = {de} } @article{LindeckeScheffler2011, author = {Lindecke, Oliver and Scheffler, Ingo}, title = {Zur Ektoparasitenfauna der Flederm{\"a}use in Sachsen Anhalt : Ectoparasites of bats in Saxony-Anhalt}, issn = {0018-0637}, year = {2011}, abstract = {During the summer 2010 several mist nettings for the monitoring of bat species were performed in Saxony-Anhalt. Captured individuals were tested for ectoparasitic infestation. The aim was to update the fauna of ectoparasites of this state and to collect data on the distribution of individual species. Regarding this, results of previous surveys are summarised. In the present study nine out of thirteen bat species were found to be infested with a total of one flea species, one species of bat flies and eight species of mites. The infestation with fleas was below the expectations. Six spinturnicid mite species out of those occurring in Germany could be ascertained for Saxony-Anhalt. These are Spinturnix acuminatus (Koch, 1836), S. andegavinus (Kolenati, 1857), S. helvetiae Deunff, Keller \& Aellen, 1986, S. mystacinus (Kolenati, 1857), S. plecotinus (Koch, 1839) and S. puncata (Sundevall, 1833). Details about the infestation with parasites (abundances) of the respective bat species are presented. Further information on the biology of spinturnicid mites are given and infestation characteristics are compared with those of other surveys. Keywords: ectoparasites, bats, Chiroptera, gamasine mite, Acari, Spinturnix, Ischnopsyllidae, Nycteribiidae, Saxony-Anhalt, Germany}, language = {de} } @article{SchefflerHiller2010, author = {Scheffler, Ingo and Hiller, Andre}, title = {Zur Ektoparasitenfauna der Flederm{\"a}use in Niedersachsen : Neue Funde am Iberg bei Bad Grund}, year = {2010}, abstract = {Bei einer aktuellen Studie am Iberg in Bad Grund konnten im September 2010 acht Fledermausarten parasitologisch untersucht werden. Die Faenge ergaben Nachweise von 11 Ektoparasitenarten (Fledermausfliegen, Floehe, Flughaut- und Ohrmilben), wobei einige davon Erstfunde fuer Niedersachsen sind. Aus den Fundangaben wurden Parasitenspektren fuer die einzelnen Wirtsarten erstellt und durch weitere unveroeffentlichte Meldungen ergaenzt. Fuer den Vergleich von Ektoparasitenspektren verschiedener Wirtsarten erfolgte die Einfuehrung einer Formel, die die Berechnung einer allgemeinen "Parasitenlast" ermoeglicht. Die Betrachtung der Verteilung von Ektoparasiten auf Individuen zeigte eine starke Trennung verschiedener Parasitengruppen, ein synchrones Vorkommen wurde nur selten registriert. Moeglicherweise ist die Konkurrenz zwischen Ektoparasitenarten ein bisher unterschaetzter determinierender Faktor fuer die Praesenz auf einem Wirt.}, language = {de} } @article{FischerHeinkenMeyeretal.2009, author = {Fischer, Petra and Heinken, Thilo and Meyer, Peter and Schmidt, Marcus and Waesch, Gunnar}, title = {Zur Abgrenzung und Situation des FFH-Lebensraumtyps "Mitteleurop{\"a}ische Flechten-Kiefernw{\"a}lder" (91TO) in Deutschland}, issn = {0028-0615}, year = {2009}, abstract = {Die in Deutschland gegenw{\"a}rtig durch N{\"a}hrstoffeintr{\"a}ge und ausbleibenden N{\"a}hrstoffentzug stark im R{\"u}ckgang begriffenen Flechten-Kiefernw{\"a}lder werden als Biotoptyp wie auch als Lebensraumtyp "Mitteleurop{\"a}ische Flechten-Kiefernw{\"a}lder" (Code 91T0) diskutiert. Die bisherige, sehr uneinheitliche Differenzierung von Flechten-Kiefernw{\"a}ldern auf der Ebene von Biotoptypen wird dargestellt. Auf der Grundlage neuerer vegetationskundlicher {\"u}bersichten werden Vorschl{\"a}ge f{\"u}r eine einheitliche Abgrenzung des Biotoptyps "Flechten-Kiefernwald" und des Lebensraumtyps 91T0 unterbreitet. Im nieders{\"a}chsischen Naturwaldreservat "Kaarßer Sandberge" (Niedersachsen) wurde die Anwendung des Konzeptes erfolgreich erprobt. Nicht nur hier, sondern auch deutschlandweit wird der R{\"u}ckgang der Erdflechten in den Kieferw{\"a}ldern zugunsten von Drahtschmiele und/ oder pleurokarpen Moosen deutlich. Nach der derzeitigen Definition des Lebensraumtyps 91T0 besteht auf der Grundlage der FFH-Richtlinie nicht f{\"u}r alle Flechten-Kiefernw{\"a}lder eine Chance der Verbesserung. Der Ausschluss von außerhalb des nat{\"u}rlichen Verbreitungsgebietes der Wald-Kiefer gelegenen sowie von durch Aufforstung angepflanzten Best{\"a}nden bringt Probleme mit sich, die diskutiert werden. F{\"u}r den Erhalt und die Wiederherstellung der gr{\"o}ßtenteils nutzungsbedingt entstandenen Flechten-Kiefernw{\"a}lder sind praktikable Pflegemaßnahmen notwendig, die im Rahmen von Streunutzungsversuchen erprobt werden m{\"u}ssen.}, language = {de} } @book{SieringBeier2001, author = {Siering, G{\"u}nter and Beier, Wolfgang}, title = {Zum Vorkommen von Bockk{\"a}fern (Col., Cerambicidae) im Gebiet des ehemaligen GUS-Truppen{\"u}bungsplatzes "Dallgow-D{\"o}beritz" bei Potsdam, 1. Nachtrag}, year = {2001}, language = {de} } @article{Kummer1996, author = {Kummer, Volker}, title = {Zum Vorkommen von Asplenium viride HUDS : in Berlin und Brandenburg}, year = {1996}, language = {de} } @article{Kummer1998, author = {Kummer, Volker}, title = {Zum Vorkommen im Cetraria islandica (.L) ACH., dem isl{\"a}ndischen Moos, und Cetraria ericetorium OPIZ in Brandenburg unter besonderer Ber{\"u}cksichtigung der Niederlausitz}, year = {1998}, language = {de} } @article{Kummer1993, author = {Kummer, Volker}, title = {Zum Vorkommen des Sternst{\"a}ublings (Mycenastrum corium) in der Niederlausitz}, year = {1993}, language = {de} } @article{RistowBurkartPrasse1997, author = {Ristow, Michael and Burkart, Michael and Prasse, R{\"u}diger}, title = {Zum Vorkommen der Bleichen Hainsimse, Luzula pallidula Kirschner (syn. L. pallescens auct.) in Brandenburg}, year = {1997}, language = {de} } @phdthesis{Otto2013, author = {Otto, Sebastian}, title = {Zulassungskonforme und schnelle massenspektronomische Analytik und pr{\"a}klinische Pharmakokinetik neuer Wirkstoffkandidaten}, address = {Potsdam}, pages = {143 S.}, year = {2013}, language = {de} } @misc{TangGladyshevDubovskayaetal.2014, author = {Tang, Kam W. and Gladyshev, Michail I. and Dubovskaya, Olgo P. and Kirillin, Georgiy and Grossart, Hans-Peter}, title = {Zooplankton carcasses and non-predatory mortality in freshwater and inland sea environments}, series = {Journal of plankton research}, volume = {36}, journal = {Journal of plankton research}, number = {3}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {0142-7873}, doi = {10.1093/plankt/fbu014}, pages = {597 -- 612}, year = {2014}, abstract = {Zooplankton carcasses are ubiquitous in marine and freshwater systems, implicating the importance of non-predatory mortality, but both are often overlooked in ecological studies compared with predatory mortality. The development of several microscopic methods allows the distinction between live and dead zooplankton in field samples, and the reported percentages of dead zooplankton average 11.6 (minimum) to 59.8 (maximum) in marine environments, and 7.4 (minimum) to 47.6 (maximum) in fresh and inland waters. Common causes of non-predatory mortality among zooplankton include senescence, temperature change, physical and chemical stresses, parasitism and food-related factors. Carcasses resulting from non-predatory mortality may undergo decomposition leading to an increase in microbial production and a shift in microbial composition in the water column. Alternatively, sinking carcasses may contribute significantly to vertical carbon flux especially outside the phytoplankton growth seasons, and become a food source for the benthos. Global climate change is already altering freshwater ecosystems on multiple levels, and likely will have significant positive or negative effects on zooplankton non-predatory mortality. Better spatial and temporal studies of zooplankton carcasses and non-predatory mortality rates will improve our understanding of this important but under-appreciated topic.}, language = {en} } @phdthesis{Kiemel2023, author = {Kiemel, Katrin}, title = {Zooplankton adaptations and community dynamics in space and time}, school = {Universit{\"a}t Potsdam}, year = {2023}, abstract = {In times of ongoing biodiversity loss, understanding how communities are structured and what mechanisms and local adaptations underlie the patterns we observe in nature is crucial for predicting how future ecological and anthropogenic changes might affect local and regional biodiversity. Aquatic zooplankton are a group of primary consumers that represent a critical link in the food chain, providing nutrients for the entire food web. Thus, understanding the adaptability and structure of zooplankton communities is essential. In this work, the genetic basis for the different temperature adaptations of two seasonally shifted (i.e., temperature-dependent) occurring freshwater rotifers of a formerly cryptic species complex (Brachionus calyciflorus) was investigated to understand the overall genetic diversity and evolutionary scenario for putative adaptations to different temperature regimes. Furthermore, this work aimed to clarify to what extent the different temperature adaptations may represent a niche partitioning process thus enabling co-existence. The findings were then embedded in a metacommunity context to understand how zooplankton communities assemble in a kettle hole metacommunity located in the northeastern German "Uckermark" and which underlying processes contribute to the biodiversity patterns we observe. Using a combined approach of newly generated mitochondrial resources (genomes/cds) and the analysis of a candidate gene (Heat Shock Protein 40kDa) for temperature adaptation, I showed that the global representatives of B. calyciflorus s.s.. are genetically more similar than B. fernandoi (average pairwise nucleotide diversity: 0.079 intraspecific vs. 0.257 interspecific) indicating that both species carry different standing genetic variation. In addition to differential expression in the thermotolerant B. calyciflorus s.s. and thermosensitive B. fernandoi, the HSP 40kDa also showed structural variation with eleven fixed and six positively selected sites, some of which are located in functional areas of the protein. The estimated divergence time of ~ 25-29 Myr combined with the fixed sites and a prevalence of ancestral amino acids in B. calyciflorus s.s. indicate that B. calyciflorus s.s. remained in the ancestral niche, while B. fernandoi partitioned into a new niche. The comparison of mitochondrial and nuclear markers (HPS 40kDa, ITS1, COI) revealed a hybridisation event between the two species. However, as hybridisation between the two species is rare, it can be concluded that the temporally isolated niches (i.e., seasonal-shifted occurrence) they inhabit based on their different temperature preferences most likely represent a pre-zygotic isolation mechanism that allows sympatric occurrence while maintaining species boundaries. To determine the processes underlying zooplankton community assembly, a zooplankton metacommunity comprising 24 kettle holes was sampled over a two-year period. Active (i.e., water samples) and dormant communities (i.e., dormant eggs hatched from sediment) were identified using a two-fragment DNA metabarcoding approach (COI and 18S). Species richness and diversity as well as community composition were analysed considering spatial, temporal and environmental parameters. The analysis revealed that environmental filtering based on parameters such as pH, size and location of the habitat patch (i.e., kettle hole) and surrounding field crops largely determined zooplankton community composition (explained variance: Bray-Curtis dissimilarities: 10.5\%; Jaccard dissimilarities: 12.9\%), indicating that adaptation to a particular habitat is a key feature of zooplankton species in this system. While the spatial configuration of the kettle holes played a minor role (explained variance: Bray-Curtis dissimilarities: 2.8\% and Jaccard dissimilarities: 5.5\%), the individual kettle hole sites had a significant influence on the community composition. This suggests monopolisation/priority effects (i.e., dormant communities) of certain species in individual kettle holes. As environmental filtering is the dominating process structuring zooplankton communities, this system could be significantly influenced by future land-use change, pollution and climate change.}, language = {en} } @article{LohmannGuoTietjen2018, author = {Lohmann, Dirk and Guo, Tong and Tietjen, Britta}, title = {Zooming in on coarse plant functional types-simulated response of savanna vegetation composition in response to aridity and grazing}, series = {Theoretical ecology}, volume = {11}, journal = {Theoretical ecology}, number = {2}, publisher = {Springer}, address = {Heidelberg}, issn = {1874-1738}, doi = {10.1007/s12080-017-0356-x}, pages = {161 -- 173}, year = {2018}, abstract = {Precipitation and land use in terms of livestock grazing have been identified as two of the most important drivers structuring the vegetation composition of semi-arid and arid savannas. Savanna research on the impact of these drivers has widely applied the so-called plant functional type (PFT) approach, grouping the vegetation into two or three broad types (here called meta-PFTs): woody plants and grasses, which are sometimes divided into perennial and annual grasses. However, little is known about the response of functional traits within these coarse types towards water availability or livestock grazing. In this study, we extended an existing eco-hydrological savanna vegetation model to capture trait diversity within the three broad meta-PFTs to assess the effects of both grazing and mean annual precipitation (MAP) on trait composition along a gradient of both drivers. Our results show a complex pattern of trait responses to grazing and aridity. The response differs for the three meta-PFTs. From our findings, we derive that trait responses to grazing and aridity for perennial grasses are similar, as suggested by the convergence model for grazing and aridity. However, we also see that this only holds for simulations below a MAP of 500 mm. This combined with the finding that trait response differs between the three meta-PFTs leads to the conclusion that there is no single, universal trait or set of traits determining the response to grazing and aridity. We finally discuss how simulation models including trait variability within meta-PFTs are necessary to understand ecosystem responses to environmental drivers, both locally and globally and how this perspective will help to extend conceptual frameworks of other ecosystems to savanna research.}, language = {en} } @phdthesis{Lengefeld2010, author = {Lengefeld, Jan}, title = {Zirkulardichroismus-Messungen mit Synchrotronstrahlung am BESSY : M{\"o}glichkeiten und Grenzen bei der Untersuchung biologischer Proben}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-44263}, school = {Universit{\"a}t Potsdam}, year = {2010}, abstract = {In dieser Arbeit wurden die M{\"o}glichkeiten und Grenzen f{\"u}r Zirkulardichroismus-Messungen mit Synchrotronstrahlung untersucht. Dazu wurde ein Messaufbau f{\"u}r Zirkulardichroismus-Messungen an zwei Strahlrohren am Berliner Elektronenspeicherring f{\"u}r Synchrotronstrahlung eingesetzt, die f{\"u}r Messungen im Bereich des ultravioletten Lichts geeignet sind. Eigenschaften der Strahlrohre und des Messaufbau wurden in einigen wichtigen Punkten mit kommerziellen Zirkulardichroismus-Spektrometern verglichen. Der Schwerpunkt lag auf der Ausdehnung des zug{\"a}nglichen Wellenl{\"a}ngenbereichs unterhalb von 180 nm zur Untersuchung des Zirkulardichroismus von Proteinen in diesem Bereich. In diesem Bereich ist es nicht nur die Lichtquelle sondern vor allem die Absorption des Lichts durch Wasser, die den Messbereich bei der Messung biologischer Proben in w{\"a}ssriger L{\"o}sung einschr{\"a}nkt. Es wurden Bedingungen gefunden, unter denen der Messbereich auf etwa 160 nm, in einigen F{\"a}llen bis auf 130 nm ausgedehnt werden konnte. Dazu musste die Pfadl{\"a}nge deutlich reduziert werden und verschieden Probenk{\"u}vetten wurden getestet. Der Einfluss der dabei auftretenden Spannungsdoppelbrechung in den Probenk{\"u}vetten auf das Messsignal konnte mit einem alternativen Messaufbau deutlich reduziert werden. Systematische Fehler im Messsignal und auftretende Strahlensch{\"a}den begrenzen jedoch die Zuverl{\"a}ssigkeit der gemessenen Spektren. Bei Proteinfilmen schr{\"a}nkt die Absorption von Wasser den Messbereich kaum ein. Es wurden jedoch meist deutliche Unterschiede zwischen den Spektren von Proteinfilmen und den Spektren von Proteinen in w{\"a}ssriger L{\"o}sung festgestellt. Solange diese Unterschiede nicht minimiert werden k{\"o}nnen, stellen Proteinfilme keine praktikable Alternative zu Messungen in w{\"a}ssriger L{\"o}sung dar.}, language = {de} } @article{BauerEremenkoEhrentreichFoersteretal.1996, author = {Bauer, Christian G. and Eremenko, A. V. and Ehrentreich-F{\"o}rster, Eva and Bier, Frank Fabian and Makower, Alexander and Halsall, H. B. and Heineman, W. R. and Scheller, Frieder W.}, title = {Zeptomole-detecting biosensor for alkaline phosphatase in an electroche mical immunoassay for 2,4- dichlorophenoacetic acid}, year = {1996}, language = {en} } @phdthesis{Brandt2001, author = {Brandt, Stephan Peter}, title = {Zelltyp-spezifische Mikroanalyse von Arabidopsis thaliana-Bl{\"a}ttern}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-0000410}, school = {Universit{\"a}t Potsdam}, year = {2001}, abstract = {Im ersten Teil der Arbeit wurden Strategien zur Analyse von Transkripten erarbeitet. Die ersten Versuche zielten darauf ab, in mit Glaskapillaren genommenen Einzelzellproben verschiedener Gewebeschichten RT-PCR durchzuf{\"u}hren, um spezifische Transkripte nachweisen zu k{\"o}nnen. Dies gelang f{\"u}r eine Reihe von Genen aus verschiedenen Pflanzenspezies. Dabei konnten sowohl Transkripte stark wie auch schwach exprimierter Gene nachgewiesen werden. F{\"u}r die Erstellung von Gewebe-spezifischen Expressionsprofilen war es notwendig, die in vereinigten Zellproben enthaltene mRNA zun{\"a}chst zu amplifizieren, um eine ausreichende Menge f{\"u}r Arrayhybridisierungen zu erhalten. Vor der Vermehrung wurde die mRNA revers transkribiert. Es wurden daran anschließend verschiedene Amplifikationsstrategien getestet: Die neben Tailing, Adapterligation und anderen PCR-basierenden Protokollen getestete Arbitrary-PCR hat sich in dieser Arbeit als einfache und einzige Methode herausgestellt, die mit so geringen cDNA-Mengen reproduzierbar arbeitet. Durch Gewebe-spezifische Array-hybridisierungen mit der so amplifizierten RNA konnten schon bekannte Expressionsmuster verschiedener Gene, vornehmlich solcher, die an der Photosynthese beteiligt sind, beobachtet werden. Es wurden aber auch eine ganze Reihe neuer offensichtlich Gewebe-spezifisch exprimierter Gene gefunden. Exemplarisch f{\"u}r die differentiell exprimierten Gene konnte das durch Arrayhybridisierungen gefundene Expressionsmuster der kleinen Untereinheit von Rubisco verifiziert werden. Hierzu wurden Methoden zum Gewebe-spezifischen Northernblot sowie semiquantitativer und Echtzeit-Einzelzell-RT-PCR entwickelt. Im zweiten Teil der Arbeit wurden Methoden zur Analyse von Metaboliten einschließlich anorganischer Ionen verwendet. Es stellte sich heraus, daß die multiparallele Methode der Gaschromatographie-Massenspektrometrie keine geeignete Methode f{\"u}r die Analyse selbst vieler vereinigter Zellinhalte ist. Daher wurde auf Kapillarelektrophorese zur{\"u}ckgegriffen. Eine Methode, die mit sehr kleinen Probenvolumina auskommt, eine hohe Trennung erzielt und zudem extrem geringe Detektionslimits besitzt. Die Analyse von Kohlenhydraten und Anionen erfordert eine weitere Optimierung. {\"U}ber UV-Detektion konnte die K+-Konzentration in verschiedenen Geweben von A. thaliana bestimmt werden. Sie lag in Epidermis und Mesophyll mit ca. 25 mM unterhalb der f{\"u}r andere Pflanzenspezies (Solanum tuberosum und Hordeum vulgare) publizierten Konzentration. Weiter konnte gezeigt werden, daß zw{\"o}lf freie Aminos{\"a}uren mittels einer auf Kapillarelektrophorese basierenden Methode in vereinigten Zellproben von Cucurbita maxima identifiziert werden konnten. Die {\"U}bertragung der Methode auf A. thaliana-Proben muß jedoch weiter optimiert werden, da die Sensitivit{\"a}t selbst bei Laser induzierter Fluoreszenz-Detektion nicht ausreichte. Im dritten und letzten Teil der Arbeit wurde eine Methode entwickelt, die die Analyse bekannter wie unbekannter Proteine in Gewebe-spezifischen Proben erm{\"o}glicht. Hierzu wurde zur Probennahme mittels mechanischer Mikrodissektion eine alternative Methode zur Laser Capture Microdissection verwendet, um aus eingebetteten Gewebeschnitten distinkte Bereiche herauszuschneiden und somit homogenes Gewebe anzureichern. Aus diesem konnten die Proteine extrahiert und {\"u}ber Polyacrylamidgelelektrophorese separariert werden. Banden konnten ausgeschnitten, tryptisch verdaut und massenspektrometrisch die Prim{\"a}rsequenz der Peptidfragmente bestimmt werden. So konnten als Hauptproteine im Mesophyll die große Untereinheit von Rubisco sowie ein Chlorophyll bindendes Protein gefunden werden. Die in dieser Arbeit entwickelten und auf die Modellpflanze Arabidopsis thaliana angewandten Einzelzellanalysetechniken erlauben es in Zukunft, physiologische Prozesse besser sowohl r{\"a}umlich als auch zeitlich aufzul{\"o}sen. Dies wird zu einem detaillierteren Verst{\"a}ndnis mannigfaltiger Vorg{\"a}nge wie Zell-Zell-Kommunikation, Signalweiterleitung oder Pflanzen-Pathogen-Interaktionen f{\"u}hren.}, language = {de} } @article{GroenkeKlepel2000, author = {Gr{\"o}nke, Ottokar and Klepel, Gert}, title = {Zelle, Einzeller, Vielzeller}, isbn = {3-06-010768-8}, year = {2000}, language = {de} } @phdthesis{Pacholsky2003, author = {Pacholsky, Dirk}, title = {Zell-Zell- und Zell-Matrix-Kontakte w{\"a}hrend der Muskelentwicklung}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-0001161}, school = {Universit{\"a}t Potsdam}, year = {2003}, abstract = {Im Rahmen dieser Arbeit wurden zwei humane Varianten des von Wang et al., 1999, erstmals beschriebenen muskelspezifischen Proteins Xin (Huhn und Maus) {\"u}ber Sequenzanalyse, Immunofluoreszenzmikroskopie, Transfektionsstudien und biochemischer Analyse n{\"a}her charakterisiert. Die Proteine wurden mit human Xin related proteins 1 und 2 - hXirp1 und 2 -bezeichnet. Die Xin-Proteine enthielten bisher unbekannte, sowie spezifische, repetitive Motive, die aus jeweils mindestens 16 Aminos{\"a}uren bestanden. Ihre Aminos{\"a}uresequenz, mit einer Vielzahl weiterer putativer Motivsequenzen, verwies auf eine potentielle Funktion von hXirp als Adapterprotein in Muskelzellen. Das hier n{\"a}her untersuchte hXirp1 lokalisierte an den Zell-Matrix-Verbindungen der Muskel-Sehnen-{\"U}bergangszone im Skelettmuskel, sowie an den Zell-Zell-Verbindungen der Glanzstreifen im Herzmuskel. W{\"a}hrend der Muskelentwicklung zeigte hXirp1 eine sehr fr{\"u}he Expression, zusammen mit einer pr{\"a}gnanten Lokalisation an den Pr{\"a}myofibrillen und deren Verankerungsstrukturen, die auf eine Funktion des Proteins in der Myofibrillogenese deuten. Ektopische Expressionen von hXirp1 in einer Vielzahl von Nichtmuskel-Kulturzellen zeigten wiederum eine Lokalisation des Proteins an den Zell-Matrix-Kontakten dieser Zellen. Am Beispiel von hXirp1 und 2 wurde stellvertretend f{\"u}r die Familie der Xin-Proteine gezeigt, daß es sich bei den repetitiven Motiven um neuartige, F-Aktin bindende Sequenzmotive handelte. Die Xin-Proteine k{\"o}nnen somit als muskelspezifische, aktinbindende, potentielle Adapterproteine bezeichnet werden, denen eine strukturelle und funktionelle Beteiligung an der Verankerung der Myofibrillen im adulten Muskel, wie auch w{\"a}hrend der Myofibrillogenese zukommt.}, language = {de} } @article{CuiLvChenetal.2015, author = {Cui, Xiao and Lv, Yang and Chen, Miaolin and Nikoloski, Zoran and Twell, David and Zhang, Dabing}, title = {Young Genes out of the Male: An Insight from Evolutionary Age Analysis of the Pollen Transcriptome}, series = {Molecular plant}, volume = {8}, journal = {Molecular plant}, number = {6}, publisher = {Cell Press}, address = {Cambridge}, issn = {1674-2052}, doi = {10.1016/j.molp.2014.12.008}, pages = {935 -- 945}, year = {2015}, abstract = {The birth of new genes in genomes is an important evolutionary event. Several studies reveal that new genes in animals tend to be preferentially expressed in male reproductive tissues such as testis (Betran et al., 2002; Begun et al., 2007; Dubruille et al., 2012), and thus an "out of testis' hypothesis for the emergence of new genes has been proposed (Vinckenbosch et al., 2006; Kaessmann, 2010). However, such phenomena have not been examined in plant species. Here, by employing a phylostratigraphic method, we dated the origin of protein-coding genes in rice and Arabidopsis thaliana and observed a number of young genes in both species. These young genes tend to encode short extracellular proteins, which may be involved in rapid evolving processes, such as reproductive barriers, species specification, and antimicrobial processes. Further analysis of transcriptome age indexes across different tissues revealed that male reproductive cells express a phylogenetically younger transcriptome than other plant tissues. Compared with sporophytic tissues, the young transcriptomes of the male gametophyte displayed greater complexity and diversity, which included a higher ratio of anti-sense and inter-genic transcripts, reflecting a pervasive transcription state that facilitated the emergence of new genes. Here, we propose that pollen may act as an "innovation incubator' for the birth of de novo genes. With cases of male-biased expression of young genes reported in animals, the "new genes out of the male' model revealed a common evolutionary force that drives reproductive barriers, species specification, and the upgrading of defensive mechanisms against pathogens.}, language = {en} } @article{MirhajBoitRazzaketal.2013, author = {Mirhaj, M. and Boit, Alice and Razzak, M. A. and Wahab, M. A.}, title = {Yield performance comparison between cultures of rice cum prawn (Macrobrachium rosenbergii) and rice cum fish (Cyprinus carpio, Oreochromis niloticus) in North-Eastern Bangladesh}, series = {Aquaculture : an international journal devoted to research on the exploration and improvement of all aquatic food resources, both floristic and faunistic, from freshwater, brackish and marine environment, related directly or indirectly to human consumption}, volume = {392}, journal = {Aquaculture : an international journal devoted to research on the exploration and improvement of all aquatic food resources, both floristic and faunistic, from freshwater, brackish and marine environment, related directly or indirectly to human consumption}, number = {5}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0044-8486}, doi = {10.1016/j.aquaculture.2013.01.038}, pages = {26 -- 33}, year = {2013}, abstract = {Integrated and concurrent cultures in rice fields are a promising approach to sustainable farming as the demand for aquacultural and agricultural products continues to grow while land and water resources become increasingly scarce. Prawn farming mainly takes place in coastal regions in improved extensive to semi-intensive aquacultures but a trend to shift the industry to inland regions has been noticed. This inland study in Northern Bangladesh used different input regimes such as fertilizer and additional feed to compare the performance of prawn and fish in flooded paddy fields in regard to water quality measurements. Maximal net yields and body weight gain with minimized negative impact on water quality were found when initial body weights of prawn were optimized. Regarding yield factors in reference to the reduction of costs due to the avoidance of expensive fertilizer/feed and effort, prawn performed better than integrated fish cultures considering a higher market value of prawn with net yields of up to 97 +/- 55 kg ha(-1) for unfed and 151 +/- 61 kg ha(-1) for fed treatments. Rice yields of up to 4.7 +/- 0.1 t ha(-1) for unfed and 4.4 +/- 0.1 t ha(-1) were achieved for fed treatments. The findings suggest that for small scale farmers, prawn cum rice cultures are an economically profitable and comparatively easily manageable alternative to rice cum fish cultures.}, language = {en} } @article{LilieBaerKettneretal.2011, author = {Lilie, Hauke and Baer, Dorit and Kettner, Karina and Weininger, Ulrich and Balbach, Jochen and Naumann, Manfred and Mueller, Eva-Christina and Otto, Albrecht and Gast, Klaus and Golbik, Ralph and Kriegel, Thomas}, title = {Yeast hexokinase isoenzyme ScHxk2 stability of a two-domain protein with discontinuous domains}, series = {Protein engineering design \& selection}, volume = {24}, journal = {Protein engineering design \& selection}, number = {1-2}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {1741-0126}, doi = {10.1093/protein/gzq098}, pages = {79 -- 87}, year = {2011}, abstract = {The hexokinase isoenzyme 2 of Saccharomyces cerevisiae (ScHxk2) represents an archetype of a two-domain protein with the active site located in a cleft between the two domains. Binding of the substrate glucose results in a rigid body movement of the two domains leading to a cleft closure of the active site. Both domains of this enzyme are composed of discontinuous peptide sequences. This structural feature is reflected in the stability and folding of the ScHxk2 protein. Structural transitions induced by urea treatment resulted in the population of a thermodynamically stable folding intermediate, which, however, does not correspond to a molecule with one domain folded and the other unfolded. As demonstrated by different spectroscopic techniques, both domains are structurally affected by the partial denaturation. The intermediate possesses only 40\% of the native secondary structural content and a substantial increase in the Stokes radius as judged by circular dichroism and dynamic light scattering analyses. One-dimensional H-1 NMR data prove that all tryptophan residues are in a non-native environment in the intermediate, indicating substantial changes in the tertiary structure. Still, the intermediate possesses quite a high stability for a transition intermediate of about Delta G = -22 kJ mol(-1).}, language = {en} } @article{LilieBaerKettneretal.2011, author = {Lilie, Hauke and B{\"a}r, Dorit and Kettner, Karina and Weininger, Ulrich and Balbach, Jochen and Naumann, Manfred and M{\"u}ller, Eva-Christina and Otto, Albrecht and Gast, Klaus and Golbik, Ralph}, title = {Yeast hexokinase isoenzyme ScHxk2 : stability of a two-domain protein with discontinuous domains}, issn = {0269-2139}, year = {2011}, abstract = {The hexokinase isoenzyme 2 of Saccharomyces cerevisiae (ScHxk2) represents an archetype of a two-domain protein with the active site located in a cleft between the two domains. Binding of the substrate glucose results in a rigid body movement of the two domains leading to a cleft closure of the active site. Both domains of this enzyme are composed of discontinuous peptide sequences. This structural feature is reflected in the stability and folding of the ScHxk2 protein. Structural transitions induced by urea treatment resulted in the population of a thermodynamically stable folding intermediate, which, however, does not correspond to a molecule with one domain folded and the other unfolded. As demonstrated by different spectroscopic techniques, both domains are structurally affected by the partial denaturation. The intermediate possesses only 40\% of the native secondary structural content and a substantial increase in the Stokes radius as judged by circular dichroism and dynamic light scattering analyses. One-dimensional 1H NMR data prove that all tryptophan residues are in a non-native environment in the intermediate, indicating substantial changes in the tertiary structure. Still, the intermediate possesses quite a high stability for a transition intermediate of about ;G = ;22 kJ mol;1.}, language = {en} } @article{AlbrechtHaebelKochetal.2004, author = {Albrecht, Tanja and Haebel, Sophie and Koch, Anke and Krause, Ulrike and Eckermann, Nora and Steup, Martin}, title = {Yeast glycogenin (Glg2p) produced in Escherichia coli is simultaneously glucosylated at two vicinal tyrosin residues but results in a reduced bacterial glycogen accumulation}, year = {2004}, abstract = {Saccharomyces cerevisiae possesses two glycogenin isoforms (designated as Glg1p and Glg2p) that both contain a conserved tyrosine residue, Tyr232. However, Glg2p possesses an additional tyrosine residue, Tyr230 and therefore two potential autoglucosylation sites. Glucosylation of Glg2p was studied using both matrix-assisted laser desorption ionization and electrospray quadrupole time of flight mass spectrometry. Glg2p, carrying a C-terminal (His(6)) tag, was produced in Escherichia coli and purified. By tryptic digestion and reversed phase chromatography a peptide (residues 219-246 of the complete Glg2p sequence) was isolated that contained 4-25 glucosyl residues. Following incubation of Glg2p with UDPglucose, more than 36 glucosyl residues were covalently bound to this peptide. Using a combination of cyanogen bromide cleavage of the protein backbone, enzymatic hydrolysis of glycosidic bonds and reversed phase chromatography, mono- and diglucosylated peptides having the sequence PNYGYQSSPAM were generated. MS/MS spectra revealed that glucosyl residues were attached to both Tyr232 and Tyr230 within the same peptide. The formation of the highly glucosylated eukaryotic Glg2p did not favour the bacterial glycogen accumulation. Under various experimental conditions Glg2p-producing cells accumulated approximately 30\% less glycogen than a control transformed with a Glg2p lacking plasmid. The size distribution of the glycogen and extractable activities of several glycogen-related enzymes were essentially unchanged. As revealed by high performance anion exchange chromatography, the intracellular maltooligosaccharide pattern of the bacterial cells expressing the functional eukaryotic transgene was significantly altered. Thus, the eukaryotic glycogenin appears to be incompatible with the bacterial initiation of glycogen biosynthesis}, language = {en} } @article{RedelbergerSedukGenestetal.2011, author = {Redelberger, David and Seduk, Farida and Genest, Olivier and Mejean, Vincent and Leimk{\"u}hler, Silke and Iobbi-Nivol, Chantal}, title = {YcdY Protein of Escherichia coli, an Atypical Member of the TorD Chaperone Family}, series = {Journal of bacteriology}, volume = {193}, journal = {Journal of bacteriology}, number = {23}, publisher = {American Society for Microbiology}, address = {Washington}, issn = {0021-9193}, doi = {10.1128/JB.05927-11}, pages = {6512 -- 6516}, year = {2011}, abstract = {The TorD family of specific chaperones is divided into four subfamilies dedicated to molybdoenzyme biogenesis and a fifth one, exemplified by YcdY of Escherichia coli, for which no defined partner has been identified so far. We propose that YcdY is the chaperone of YcdX, a zinc protein involved in the swarming motility process of E. coli, since YcdY interacts with YcdX and increases its activity in vitro.}, language = {en} } @article{PacholskyVakeelHimmeletal.2004, author = {Pacholsky, Dirk and Vakeel, Padmanabhan and Himmel, Mirko and Lowe, T. and Stradal, T. and Rottner, K. and F{\"u}rst, Dieter Oswald and vanderVen, Peter F. M.}, title = {Xin repeats define a novel actin-binding motif}, issn = {0021-9533}, year = {2004}, abstract = {Xin is a protein that is expressed during early developmental stages of cardiac and skeletal muscles. Immunolocalization studies indicated a peripheral localization in embryonic mouse heart, where Xin localizes with beta- catenin and N-cadherin. In adult tissues, Xin is found primarily in the intercalated discs of cardiomyocytes and the myotendinous junctions of skeletal muscle cells, both specialized attachment sites of the myofibrillar ends to the sarcolemma. A large part of the Xin protein consists of unique 16 amino acid repeats with unknown function. We have investigated the characteristics of the Xin repeats by transfection experiments and actin-binding assays and ascertained that, upon expression in cultured cells, these repeats bind to and stabilize the actin-based cytoskeleton. In vitro co- sedimentation assays with skeletal muscle actin indicated that they not only directly bind actin filaments, but also have the capability of arranging microfilaments into networks that sediment upon low-speed centrifugation. Very similar repeats were also found in Xin-repeat protein 2' (XIRP2), a novel protein that seems to be expressed mainly in striated muscles. Human XIRP2 contains 28 Xin repeats with properties identical to those of Xin. We conclude that the Xin repeats define a novel, repetitive actin-binding motif present in at least two different muscle proteins. These Xin- repeat proteins therefore constitute the first two members of a novel family of actin-binding proteins}, language = {en} } @article{KalimuthuLeimkuehlerBernhardt2011, author = {Kalimuthu, Palraj and Leimk{\"u}hler, Silke and Bernhardt, Paul V.}, title = {Xanthine dehydrogenase electrocatalysis autocatalysis and novel activity}, series = {The journal of physical chemistry : B, Condensed matter, materials, surfaces, interfaces \& biophysical chemistry}, volume = {115}, journal = {The journal of physical chemistry : B, Condensed matter, materials, surfaces, interfaces \& biophysical chemistry}, number = {11}, publisher = {American Chemical Society}, address = {Washington}, issn = {1520-6106}, doi = {10.1021/jp111809f}, pages = {2655 -- 2662}, year = {2011}, abstract = {The enzyme xanthine dehydrogenase (XDH) from the purple photosynthetic bacterium Rhodobacter capsulatus catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid as part of purine metabolism. The native electron acceptor is NAD(+) but herein we show that uric acid in its 2-electron oxidized form is able to act as an artificial electron acceptor from XDH in an electrochemically driven catalytic system. Hypoxanthine oxidation is also observed with the novel production of uric acid in a series of two consecutive 2-electron oxidation reactions via xanthine. XDH exhibits native activity in terms of its pH optimum and inhibition by allopurinol.}, language = {en} } @article{SchubertSchluckebierBackmannetal.1994, author = {Schubert, Dieter and Schluckebier, Gerd and Backmann, Jan and Granzien, Joachim and Kisker, Caroline and Choe, Hui-Woog and Hahn, Ulrich and Pfeil, Wolfgang and Saenger, Wolfram}, title = {X-ray crystallographic and calorimetric studies of the effects of the mutation Trp59Tyr in ribonuclease T1}, year = {1994}, language = {en} } @misc{PratHajnýGrunewaldetal.2018, author = {Pr{\´a}t, Tom{\´a}š and Hajny', Jakub and Grunewald, Wim and Vasileva, Mina and Moln{\´a}r, Gergely and Tejos, Ricardo and Schmid, Markus and Sauer, Michael and Friml, Jiř{\´i}}, title = {WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {1123}, issn = {1866-8372}, doi = {10.25932/publishup-44633}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-446331}, pages = {20}, year = {2018}, abstract = {Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17-and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain-and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development.}, language = {en} } @article{PratHajnyGrunewaldetal.2018, author = {Prat, Tomas and Hajny, Jakub and Grunewald, Wim and Vasileva, Mina and Molnar, Gergely and Tejos, Ricardo and Schmid, Markus and Sauer, Michael and Friml, Jiř{\´i}}, title = {WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity}, series = {PLoS Genetics : a peer-reviewed, open-access journal}, volume = {14}, journal = {PLoS Genetics : a peer-reviewed, open-access journal}, number = {1}, publisher = {PLoS}, address = {San Fransisco}, issn = {1553-7404}, doi = {10.1371/journal.pgen.1007177}, pages = {18}, year = {2018}, abstract = {Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17-and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain-and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development.}, language = {en} } @article{KlingstromSoldatovaStevensetal.2013, author = {Klingstrom, Tomas and Soldatova, Larissa and Stevens, Robert and Roos, T. Erik and Swertz, Morris A. and M{\"u}ller, Kristian M. and Kalas, Matus and Lambrix, Patrick and Taussig, Michael J. and Litton, Jan-Eric and Landegren, Ulf and Bongcam-Rudloff, Erik}, title = {Workshop on laboratory protocol standards for the molecular methods database}, series = {New biotechnology}, volume = {30}, journal = {New biotechnology}, number = {2}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1871-6784}, doi = {10.1016/j.nbt.2012.05.019}, pages = {109 -- 113}, year = {2013}, abstract = {Management of data to produce scientific knowledge is a key challenge for biological research in the 21st century. Emerging high-throughput technologies allow life science researchers to produce big data at speeds and in amounts that were unthinkable just a few years ago. This places high demands on all aspects of the workflow: from data capture (including the experimental constraints of the experiment), analysis and preservation, to peer-reviewed publication of results. Failure to recognise the issues at each level can lead to serious conflicts and mistakes; research may then be compromised as a result of the publication of non-coherent protocols, or the misinterpretation of published data. In this report, we present the results from a workshop that was organised to create an ontological data-modelling framework for Laboratory Protocol Standards for the Molecular Methods Database (MolMeth). The workshop provided a set of short- and long-term goals for the MolMeth database, the most important being the decision to use the established EXACT description of biomedical ontologies as a starting point.}, language = {en} } @misc{ParaskevopoulouDennisWeithoffetal.2019, author = {Paraskevopoulou, Sofia and Dennis, Alice B. and Weithoff, Guntram and Hartmann, Stefanie and Tiedemann, Ralph}, title = {Within species expressed genetic variability and gene expression response to different temperatures in the rotifer Brachionus calyciflorus sensu stricto}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {796}, issn = {1866-8372}, doi = {10.25932/publishup-44105}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-441050}, pages = {23}, year = {2019}, abstract = {Genetic divergence is impacted by many factors, including phylogenetic history, gene flow, genetic drift, and divergent selection. Rotifers are an important component of aquatic ecosystems, and genetic variation is essential to their ongoing adaptive diversification and local adaptation. In addition to coding sequence divergence, variation in gene expression may relate to variable heat tolerance, and can impose ecological barriers within species. Temperature plays a significant role in aquatic ecosystems by affecting species abundance, spatio-temporal distribution, and habitat colonization. Recently described (formerly cryptic) species of the Brachionus calyciflorus complex exhibit different temperature tolerance both in natural and in laboratory studies, and show that B. calyciflorus sensu stricto (s.s.) is a thermotolerant species. Even within B. calyciflorus s.s., there is a tendency for further temperature specializations. Comparison of expressed genes allows us to assess the impact of stressors on both expression and sequence divergence among disparate populations within a single species. Here, we have used RNA-seq to explore expressed genetic diversity in B. calyciflorus s.s. in two mitochondrial DNA lineages with different phylogenetic histories and differences in thermotolerance. We identify a suite of candidate genes that may underlie local adaptation, with a particular focus on the response to sustained high or low temperatures. We do not find adaptive divergence in established candidate genes for thermal adaptation. Rather, we detect divergent selection among our two lineages in genes related to metabolism (lipid metabolism, metabolism of xenobiotics).}, language = {en} } @article{ParaskevopoulouDennisWeithoffetal.2019, author = {Paraskevopoulou, Sofia and Dennis, Alice B. and Weithoff, Guntram and Hartmann, Stefanie and Tiedemann, Ralph}, title = {Within species expressed genetic variability and gene expression response to different temperatures in the rotifer Brachionus calyciflorus sensu stricto}, series = {PLoS ONE}, volume = {9}, journal = {PLoS ONE}, number = {14}, publisher = {PLoS ONE}, address = {San Francisco, California}, issn = {1932-6203}, doi = {10.1371/journal.pone.0223134}, pages = {21}, year = {2019}, abstract = {Genetic divergence is impacted by many factors, including phylogenetic history, gene flow, genetic drift, and divergent selection. Rotifers are an important component of aquatic ecosystems, and genetic variation is essential to their ongoing adaptive diversification and local adaptation. In addition to coding sequence divergence, variation in gene expression may relate to variable heat tolerance, and can impose ecological barriers within species. Temperature plays a significant role in aquatic ecosystems by affecting species abundance, spatio-temporal distribution, and habitat colonization. Recently described (formerly cryptic) species of the Brachionus calyciflorus complex exhibit different temperature tolerance both in natural and in laboratory studies, and show that B. calyciflorus sensu stricto (s.s.) is a thermotolerant species. Even within B. calyciflorus s.s., there is a tendency for further temperature specializations. Comparison of expressed genes allows us to assess the impact of stressors on both expression and sequence divergence among disparate populations within a single species. Here, we have used RNA-seq to explore expressed genetic diversity in B. calyciflorus s.s. in two mitochondrial DNA lineages with different phylogenetic histories and differences in thermotolerance. We identify a suite of candidate genes that may underlie local adaptation, with a particular focus on the response to sustained high or low temperatures. We do not find adaptive divergence in established candidate genes for thermal adaptation. Rather, we detect divergent selection among our two lineages in genes related to metabolism (lipid metabolism, metabolism of xenobiotics).}, language = {en} } @article{Raatz2019, author = {Raatz, Larissa}, title = {Wirtschaften in einer reich strukturierten Landschaft - geht das ?}, series = {Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018}, journal = {Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018}, publisher = {oerding print GmbH}, address = {Braunschweig}, pages = {32 -- 33}, year = {2019}, language = {de} } @phdthesis{Rietdorf2003, author = {Rietdorf, Katja}, title = {Wirkungen biogener Amine auf die Erregungs-Sekretions-Kopplung in der Speicheldr{\"u}se von Periplaneta americana (L.)}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-0000878}, school = {Universit{\"a}t Potsdam}, year = {2003}, abstract = {In der vorliegenden Arbeit habe ich wichtige Teilmechanismen der Erregungs-Sekretionskopplung in der Speicheldr{\"u}se der Schabe Periplaneta americana (L.) untersucht. Die Speicheldr{\"u}se ist von dopaminergen und serotonergen Fasern innerviert (Baumann et al., 2002). Beide Transmitter stimulieren eine unterschiedliche Reaktion der Dr{\"u}se: Dopamin (DA) stimuliert die P-Zellen der Acini und die Ausf{\"u}hrgangzellen, w{\"a}hrend Serotonin (5-HT) die P- und C-Zellen der Acini stimuliert, nicht jedoch die Ausf{\"u}hrgangzellen. Der Endspeichel ist nach einer DA-Stimulierung proteinfrei. Dagegen enth{\"a}lt er nach einer 5-HT-Stimulierung Proteine, die von den C-Zellen sezerniert werden (Just \& Walz, 1996). Im ersten Teil meiner Arbeit habe ich mittels Kapillarelektrophoretischer Analyse (CE-Analyse) die Elektrolytkonzentrationen im Endspeichel untersucht sowie die Raten der Fl{\"u}ssigkeitssekretion gemessen. Damit wollte ich kl{\"a}ren, welche Transporter an der Sekretion des Prim{\"a}rspeichels und an dessen Modifikation beteiligt sind. Ausserdem wollte ich die Rolle der transportaktiven Epithelzellen der Ausf{\"u}hrg{\"a}nge f{\"u}r die Modifikation des Prim{\"a}rspeichels untersuchen. Daf{\"u}r habe ich einen Vergleich der Elektrolytkonzentrationen im DA- und 5-HT-stimulierten Endspeichel durchgef{\"u}hrt. Der Elektrolytgehalt des DA- und 5-HT-stimulierten Endspeichels unterscheidet sich nicht signifikant voneinander. Er ist nach beiden Stimulierungen hypoosmotisch zum verwendeten Ringer. Die Ausf{\"u}hrgangzellen werden durch DA stimuliert und modifizieren den Prim{\"a}rspeichel durch eine netto-Ionenreabsorption. Meine Versuche zeigen jedoch, dass auch die w{\"a}hrend einer 5-HT-Stimulierung der Dr{\"u}se unstimulierten Ausf{\"u}hrgangzellen den Prim{\"a}rspeichel modifizieren. In einer nachfolgenden Versuchsreihe habe ich den Einfluss von Ouabain, einem Hemmstoff der Na+-K+-ATPase, und Bumetanid, einem Hemmstoff des NKCC, auf die Raten der Fl{\"u}ssigkeitssekretion sowie den Elektrolytgehalt des Endspeichels untersucht. Ich habe gefunden, dass die Aktivit{\"a}t der Na+-K+-ATPase wichtig f{\"u}r die Modifikation des DA-stimulierten Prim{\"a}rspeichels ist. Im Gegensatz dazu ist sie f{\"u}r die Modifikation des 5-HT-stimulierten Prim{\"a}rspeichels nicht von Bedeutung. Bez{\"u}glich der Fl{\"u}ssigkeitssekretion habe ich keinen Einfluss der Na+-K+-ATPase-Aktivit{\"a}t auf die DA-stimulierten Sekretionsraten gefunden, dagegen ist die 5-HT-stimulierte Sekretionsrate in Anwesenheit von Ouabain gesteigert. Die Aktivit{\"a}t des NKCC ist f{\"u}r beide sekretorische Prozesse, die Ionen- und die Fl{\"u}ssigkeitssekretion, wichtig. Eine Hemmung des NKCC bewirkt eine signifikante Verringerung der Raten der Fl{\"u}ssigkeitssekretion nach DA- und 5-HT-Stimulierung sowie in beiden F{\"a}llen einen signifikanten Abfall der Ionenkonzentrationen im Endspeichel. Im zweiten Teil meiner Arbeit habe ich versucht, {\"A}nderungen der intrazellul{\"a}ren Ionenkonzentrationen in den Acinuszellen w{\"a}hrend einer DA- oder 5-HT-Stimulierung zu messen. Diese Experimente sollten mit der Methode des \"ratiometric imaging\" durchgef{\"u}hrt werden. Messungen mit dem Ca2+-sensitiven Fluoreszenzfarbstoff Fura-2 zeigten keinen globalen Anstieg in der intrazellul{\"a}ren Ca2+-Konzentration der P-Zellen. Aufgrund von Problemen mit einer schlechten Beladung der Zellen, einer starken und sich w{\"a}hrend der Stimulierung {\"a}ndernden Autofluoreszenz der Zellen sowie {\"A}nderungen im Zellvolumen wurden keine Messungen mit Na+- und K+-sensitiven Fluoreszenzfarbstoffen durchgef{\"u}hrt. Im dritten Teil dieser Arbeit habe ich die intrazellul{\"a}ren Signalwege untersucht, die zwischen einer 5-HT-Stimulierung der Dr{\"u}se und der Proteinsekretion vermitteln. Dazu wurde der Proteingehalt im Endspeichel biochemisch mittels eines modifizierten Bradford Assay gemessen. Eine erstellte Dosis-Wirkungskurve zeigt, dass die Rate der Proteinsekretion von der zur Stimulierung verwendeten 5-HT-Konzentration abh{\"a}ngt. In einer Serie von Experimenten habe ich die intrazellul{\"a}ren Konzentrationen von Ca2+, cAMP und / oder cGMP erh{\"o}ht und anschließend den Proteingehalt im Endspeichel gemessen. Ein Anstieg der intrazellul{\"a}ren Ca2+-Konzentration aktiviert nur eine geringe Rate der Proteinsekretion. Dagegen kann die Steigerung der intrazellul{\"a}ren cAMP-Konzentration eine st{\"a}rkere Proteinsekretion aktivieren, die sich nicht signifikant von der nach 5-HT-Stimulierung unterscheidet. Die cAMP-stimulierte Proteinsekretion kann durch gleichzeitige Erh{\"o}hung der intrazellul{\"a}ren Ca2+-Konzentration weiter gesteigert werden. Dagegen aktivierte eine Erh{\"o}hung der intrazellul{\"a}ren cGMP-Konzentration die Proteinsekretion nicht. Aufgrund dieser Ergebnisse postuliere ich die Existenz eines die Adenylatcyclase aktivierenden 5-HT-Rezeptors in der Basolateralmembran der C-Zellen.}, language = {de} } @phdthesis{Meyer2018, author = {Meyer, Susann}, title = {Wirkung und Wirkungsweise von Ectoin auf DNA-Molek{\"u}le}, school = {Universit{\"a}t Potsdam}, pages = {103}, year = {2018}, language = {de} } @article{PinyouRuffPoelleretal.2016, author = {Pinyou, Piyanut and Ruff, Adrian and Poeller, Sascha and Alsaoub, Sabine and Leimk{\"u}hler, Silke and Wollenberger, Ursula and Schuhmann, Wolfgang}, title = {Wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces via entrapment in low potential phenothiazine-modified redox polymers}, series = {Bioelectrochemistry : an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry ; official journal of the Bioelectrochemical Society}, volume = {109}, journal = {Bioelectrochemistry : an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry ; official journal of the Bioelectrochemical Society}, publisher = {Elsevier}, address = {Lausanne}, issn = {1567-5394}, doi = {10.1016/j.bioelechem.2015.12.005}, pages = {24 -- 30}, year = {2016}, abstract = {Phenothiazine-modified redox hydrogels were synthesized and used for the wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces. The effects of the pH value and electrode surface modification on the biocatalytic activity of the layers were studied in the presence of vanillin as the substrate. The enzyme electrodes were successfully employed as bioanodes in vanillin/O-2 biofuel cells in combination with a high potential bilirubin oxidase biocathode. Open circuit voltages of around 700 mV could be obtained in a two compartment biofuel cell setup. Moreover, the use of a rather hydrophobic polymer with a high degree of crosslinking sites ensures the formation of stable polymer/enzyme films which were successfully used as bioanode in membrane-less biofuel cells. (C) 2015 Elsevier B.V. All rights reserved.}, language = {en} } @article{Rathgeber1994, author = {Rathgeber, J{\"o}rg}, title = {Winterliche Beobachtungen an der Rohrdommel in Berlin}, issn = {0028-1301}, year = {1994}, language = {de} } @article{VoigtKaiserLooketal.2022, author = {Voigt, Christian C. and Kaiser, Klara and Look, Samantha and Scharnweber, Inga Kristin and Scholz, Carolin}, title = {Wind turbines without curtailment produce large numbers of bat fatalities throughout their lifetime}, series = {Global ecology and conservation}, volume = {37}, journal = {Global ecology and conservation}, publisher = {Elsevier}, address = {Amsterdam}, issn = {2351-9894}, doi = {10.1016/j.gecco.2022.e02149}, pages = {10}, year = {2022}, abstract = {Bats are protected by national and international legislation in European countries, yet many species, particularly migratory aerial insectivores, collide with wind turbines which counteracts conservation efforts. Within the European Union it is legally required to curtail the operation of wind turbines at periods of high bat activity, yet this is not practiced at old wind turbines. Based on data from the national carcass repository in Germany and from our own carcass searches at a wind park with three turbines west of Berlin, we evaluated the magnitude of bat casualties at old, potentially poor-sited wind turbines operating without curtailment. We report 88 documented bat carcasses collected by various searchers over the 20-year operation period of this wind park from 2001 to 2021. Common noctule bats (Nyctalus noctula) and common pipistrelles (Pipistrellus pipistrellus) were most often found dead at these turbines. Our search campaign in August and September 2021 yielded a total of 18 carcasses. We estimated that at least 209 bats were likely killed during our field survey, yielding more than 70 casualties/wind turbine or 39 casualties/ MW in two months. Since our campaign covered only part of the migration season, we consider this value as an underestimate. The 20-year period of the wind park emphasises the substantial impact old turbines may have on bat individuals and populations when operating without curtailments. We call for reconsidering the operation procedures of old wind turbines to stop the continuous loss of bats in Germany and other countries where turbine curtailments are even less practiced than in Germany.}, language = {en} } @article{WergerBergmannWeberetal.2020, author = {Werger, Luise and Bergmann, Joana and Weber, Ewald and Heinze, Johannes}, title = {Wind intensity affects fine root morphological traits with consequences for plant-soil feedback effects}, series = {Annals of Botany Plants}, volume = {12}, journal = {Annals of Botany Plants}, number = {5}, publisher = {Oxford University Press}, address = {Oxford}, issn = {2041-2851}, doi = {10.1093/aobpla/plaa050}, pages = {12}, year = {2020}, abstract = {Wind influences the development, architecture and morphology of plant roots and may modify subsequent interactions between plants and soil (plant-soil feedbacks—PSFs). However, information on wind effects on fine root morphology is scarce and the extent to which wind changes plant-soil interactions remains unclear. Therefore, we investigated the effects of two wind intensity levels by manipulating surrounding vegetation height in a grassland PSF field experiment. We grew four common plant species (two grasses and two non-leguminous forbs) with soil biota either previously conditioned by these or other species and tested the effect of wind on root:shoot ratio, fine root morphological traits as well as the outcome for PSFs. Wind intensity did not affect biomass allocation (i.e. root:shoot ratio) in any species. However, fine-root morphology of all species changed under high wind intensity. High wind intensity increased specific root length and surface area and decreased root tissue density, especially in the two grasses. Similarly, the direction of PSFs changed under high wind intensity in all four species, but differences in biomass production on the different soils between high and low wind intensity were marginal and most pronounced when comparing grasses with forbs. Because soils did not differ in plant-available nor total nutrient content, the results suggest that wind-induced changes in root morphology have the potential to influence plant-soil interactions. Linking wind-induced changes in fine-root morphology to effects on PSF improves our understanding of plant-soil interactions under changing environmental conditions.}, language = {en} } @misc{WergerBergmannWeberetal.2020, author = {Werger, Luise and Bergmann, Joana and Weber, Ewald and Heinze, Johannes}, title = {Wind intensity affects fine root morphological traits with consequences for plant-soil feedback effects}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {1019}, issn = {1866-8372}, doi = {10.25932/publishup-48409}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-484092}, pages = {14}, year = {2020}, abstract = {Wind influences the development, architecture and morphology of plant roots and may modify subsequent interactions between plants and soil (plant-soil feedbacks—PSFs). However, information on wind effects on fine root morphology is scarce and the extent to which wind changes plant-soil interactions remains unclear. Therefore, we investigated the effects of two wind intensity levels by manipulating surrounding vegetation height in a grassland PSF field experiment. We grew four common plant species (two grasses and two non-leguminous forbs) with soil biota either previously conditioned by these or other species and tested the effect of wind on root:shoot ratio, fine root morphological traits as well as the outcome for PSFs. Wind intensity did not affect biomass allocation (i.e. root:shoot ratio) in any species. However, fine-root morphology of all species changed under high wind intensity. High wind intensity increased specific root length and surface area and decreased root tissue density, especially in the two grasses. Similarly, the direction of PSFs changed under high wind intensity in all four species, but differences in biomass production on the different soils between high and low wind intensity were marginal and most pronounced when comparing grasses with forbs. Because soils did not differ in plant-available nor total nutrient content, the results suggest that wind-induced changes in root morphology have the potential to influence plant-soil interactions. Linking wind-induced changes in fine-root morphology to effects on PSF improves our understanding of plant-soil interactions under changing environmental conditions.}, language = {en} } @article{BuschKlausSchaeferetal.2019, author = {Busch, Verena and Klaus, Valentin Helmut and Schaefer, Deborah and Prati, Daniel and Boch, Steffen and M{\"u}ller, J{\"o}rg and Chiste, Melanie and Mody, Karsten and Bl{\"u}thgen, Nico and Fischer, Markus and H{\"o}lzel, Norbert and Kleinebecker, Till}, title = {Will I stay or will I go? Plant species-specific response and tolerance to high land-use intensity in temperate grassland ecosystems}, series = {Journal of vegetation science}, volume = {30}, journal = {Journal of vegetation science}, number = {4}, publisher = {Wiley}, address = {Hoboken}, issn = {1100-9233}, doi = {10.1111/jvs.12749}, pages = {674 -- 686}, year = {2019}, language = {en} } @article{GluecklerHerzschuhKruseetal.2021, author = {Gl{\"u}ckler, Ramesh and Herzschuh, Ulrike and Kruse, Stefan and Andreev, Andrei and Vyse, Stuart Andrew and Winkler, Bettina and Biskaborn, Boris and Pestryakova, Luidmila Agafyevna and Dietze, Elisabeth}, title = {Wildfire history of the boreal forest of south-western Yakutia (Siberia) over the last two millennia documented by a lake-sediment charcoal record}, series = {Biogeosciences : BG / European Geosciences Union}, volume = {18}, journal = {Biogeosciences : BG / European Geosciences Union}, number = {13}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1726-4170}, doi = {10.5194/bg-18-4185-2021}, pages = {4185 -- 4209}, year = {2021}, abstract = {Wildfires, as a key disturbance in forest ecosystems, are shaping the world's boreal landscapes. Changes in fire regimes are closely linked to a wide array of environmental factors, such as vegetation composition, climate change, and human activity. Arctic and boreal regions and, in particular, Siberian boreal forests are experiencing rising air and ground temperatures with the subsequent degradation of permafrost soils leading to shifts in tree cover and species composition. Compared to the boreal zones of North America or Europe, little is known about how such environmental changes might influence long-term fire regimes in Russia. The larch-dominated eastern Siberian deciduous boreal forests differ markedly from the composition of other boreal forests, yet data about past fire regimes remain sparse. Here, we present a high-resolution macroscopic charcoal record from lacustrine sediments of Lake Khamra (southwest Yakutia, Siberia) spanning the last ca. 2200 years, including information about charcoal particle sizes and morphotypes. Our results reveal a phase of increased charcoal accumulation between 600 and 900 CE, indicative of relatively high amounts of burnt biomass and high fire frequencies. This is followed by an almost 900-year-long period of low charcoal accumulation without significant peaks likely corresponding to cooler climate conditions. After 1750 CE fire frequencies and the relative amount of biomass burnt start to increase again, coinciding with a warming climate and increased anthropogenic land development after Russian colonization. In the 20th century, total charcoal accumulation decreases again to very low levels despite higher fire frequency, potentially reflecting a change in fire management strategies and/or a shift of the fire regime towards more frequent but smaller fires. A similar pattern for different charcoal morphotypes and comparison to a pollen and non-pollen palynomorph (NPP) record from the same sediment core indicate that broad-scale changes in vegetation composition were probably not a major driver of recorded fire regime changes. Instead, the fire regime of the last two millennia at Lake Khamra seems to be controlled mainly by a combination of short-term climate variability and anthropogenic fire ignition and suppression.}, language = {en} } @article{MrochenSchulzFischeretal.2018, author = {Mrochen, Daniel M. and Schulz, Daniel and Fischer, Stefan and Jeske, Kathrin and El Gohary, Heba and Reil, Daniela and Imholt, Christian and Truebe, Patricia and Suchomel, Josef and Tricaud, Emilie and Jacob, Jens and Heroldova, Marta and Br{\"o}ker, Barbara M. and Strommenger, Birgit and Walther, Birgit and Ulrich, Rainer G. and Holtfreter, Silva}, title = {Wild rodents and shrews are natural hosts of Staphylococcus aureus}, series = {International Journal of Medical Microbiology}, volume = {308}, journal = {International Journal of Medical Microbiology}, number = {6}, publisher = {Elsevier}, address = {Jena}, issn = {1438-4221}, doi = {10.1016/j.ijmm.2017.09.014}, pages = {590 -- 597}, year = {2018}, abstract = {Laboratory mice are the most commonly used animal model for Staphylococcus aureus infection studies. We have previously shown that laboratory mice from global vendors are frequently colonized with S. aureus. Laboratory mice originate from wild house mice. Hence, we investigated whether wild rodents, including house mice, as well as shrews are naturally colonized with S. aureus and whether S. aureus adapts to the wild animal host. 295 animals of ten different species were caught in different locations over four years (2012-2015) in Germany, France and the Czech Republic. 45 animals were positive for S. aureus (15.3\%). Three animals were co-colonized with two different isolates, resulting in 48 S. aureus isolates in total. Positive animals were found in Germany and the Czech Republic in each studied year. The S. aureus isolates belonged to ten different spa types, which grouped into six lineages (clonal complex (CC) 49, CC88, CC130, CC1956, sequence type (ST) 890, ST3033). CC49 isolates were most abundant (17/48, 35.4\%), followed by CC1956 (14/48, 29.2\%) and ST890 (9/48, 18.8\%). The wild animal isolates lacked certain properties that are common among human isolates, e.g., a phage-encoded immune evasion cluster, superantigen genes on mobile genetic elements and antibiotic resistance genes, which suggests long-term adaptation to the wild animal host. One CC130 isolate contained the mecC gene, implying wild rodents might be both reservoir and vector for methicillin-resistant. In conclusion, we demonstrated that wild rodents and shrews are naturally colonized with S. aureus, and that those S. aureus isolates show signs of host adaptation.}, language = {en} } @article{Knoesche2008, author = {Kn{\"o}sche, R{\"u}diger}, title = {Wiederfund von Najas marina L. ssp. marina im Schollener See (Elbe-Havel-Winkel, Sachsen-Anhalt)}, issn = {1432-8038}, year = {2008}, language = {de} } @book{BeierHeilmann2001, author = {Beier, Wolfgang and Heilmann, Dieter}, title = {Wiederfund von Meloe coriarius (Brandt\&Erichson, 1832 (Col., Meloidae) f{\"u}r Deutschland sowie Nachweise weiterer bemerkenswerter K{\"a}ferarten bei Lebus an der Oder (Land Brandenburg)}, year = {2001}, language = {de} } @article{Greil2001, author = {Greil, Holle}, title = {Wie weit sind wir der DIN 33402 entwachsen?}, series = {Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakult{\"a}t der Universit{\"a}t Potsdam}, volume = {10}, journal = {Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakult{\"a}t der Universit{\"a}t Potsdam}, issn = {1434-2375}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-3870}, pages = {7 -- 22}, year = {2001}, language = {de} } @article{Greil2001, author = {Greil, Holle}, title = {Wie weit sind wir der DIN 33402 entwachsen ?}, year = {2001}, language = {de} } @article{LitwinColangeli2019, author = {Litwin, Magdalena and Colangeli, Pierluigi}, title = {Wie und wohin reisen Wasserfl{\"o}he?}, series = {Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018}, journal = {Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018}, publisher = {oerding print GmbH}, address = {Braunschweig}, pages = {28 -- 29}, year = {2019}, language = {de} } @article{BjornerasWeyhenmeyerEvansetal.2017, author = {Bjorneras, C. and Weyhenmeyer, G. A. and Evans, C. D. and Gessner, M. O. and Großart, Hans-Peter and Kangur, K. and Kokorite, I. and Kortelainen, P. and Laudon, H. and Lehtoranta, J. and Lottig, N. and Monteith, D. T. and Noges, P. and Noges, T. and Oulehle, F. and Riise, G. and Rusak, J. A. and Raike, A. and Sire, J. and Sterling, S. and Kritzberg, E. S.}, title = {Widespread Increases in Iron Concentration in European and North American Freshwaters}, series = {Global biogeochemical cycles}, volume = {31}, journal = {Global biogeochemical cycles}, publisher = {American Geophysical Union}, address = {Washington}, issn = {0886-6236}, doi = {10.1002/2017GB005749}, pages = {1488 -- 1500}, year = {2017}, abstract = {Recent reports of increasing iron (Fe) concentrations in freshwaters are of concern, given the fundamental role of Fe in biogeochemical processes. Still, little is known about the frequency and geographical distribution of Fe trends or about the underlying drivers. We analyzed temporal trends of Fe concentrations across 340 water bodies distributed over 10 countries in northern Europe and North America in order to gain a clearer understanding of where, to what extent, and why Fe concentrations are on the rise. We found that Fe concentrations have significantly increased in 28\% of sites, and decreased in 4\%, with most positive trends located in northern Europe. Regions with rising Fe concentrations tend to coincide with those with organic carbon (OC) increases. Fe and OC increases may not be directly mechanistically linked, but may nevertheless be responding to common regional-scale drivers such as declining sulfur deposition or hydrological changes. A role of hydrological factors was supported by covarying trends in Fe and dissolved silica, as these elements tend to stem from similar soil depths. A positive relationship between Fe increases and conifer cover suggests that changing land use and expanded forestry could have contributed to enhanced Fe export, although increases were also observed in nonforested areas. We conclude that the phenomenon of increasing Fe concentrations is widespread, especially in northern Europe, with potentially significant implications for wider ecosystem biogeochemistry, and for the current browning of freshwaters.}, language = {en} } @article{RaatzPirhoferWalzlMuelleretal.2021, author = {Raatz, Larissa and Pirhofer-Walzl, Karin and M{\"u}ller, Marina E.H. and Scherber, Christoph and Joshi, Jasmin Radha}, title = {Who is the culprit: Is pest infestation responsible for crop yield losses close to semi-natural habitats?}, series = {Ecology and Evolution}, volume = {11}, journal = {Ecology and Evolution}, edition = {19}, publisher = {Wiley-Blackwell}, address = {Oxford}, issn = {1467-6435}, doi = {10.1002/ece3.8046}, pages = {13232 -- 13246}, year = {2021}, abstract = {Semi-natural habitats (SNHs) are becoming increasingly scarce in modern agricultural landscapes. This may reduce natural ecosystem services such as pest control with its putatively positive effect on crop production. In agreement with other studies, we recently reported wheat yield reductions at field borders which were linked to the type of SNH and the distance to the border. In this experimental landscape-wide study, we asked whether these yield losses have a biotic origin while analyzing fungal seed and fungal leaf pathogens, herbivory of cereal leaf beetles, and weed cover as hypothesized mediators between SNHs and yield. We established experimental winter wheat plots of a single variety within conventionally managed wheat fields at fixed distances either to a hedgerow or to an in-field kettle hole. For each plot, we recorded the fungal infection rate on seeds, fungal infection and herbivory rates on leaves, and weed cover. Using several generalized linear mixed-effects models as well as a structural equation model, we tested the effects of SNHs at a field scale (SNH type and distance to SNH) and at a landscape scale (percentage and diversity of SNHs within a 1000-m radius). In the dry year of 2016, we detected one putative biotic culprit: Weed cover was negatively associated with yield values at a 1-m and 5-m distance from the field border with a SNH. None of the fungal and insect pests, however, significantly affected yield, neither solely nor depending on type of or distance to a SNH. However, the pest groups themselves responded differently to SNH at the field scale and at the landscape scale. Our findings highlight that crop losses at field borders may be caused by biotic culprits; however, their negative impact seems weak and is putatively reduced by conventional farming practices.}, language = {en} } @article{RaatzPirhoferWalzlMuelleretal.2021, author = {Raatz, Larissa and Pirhofer-Walzl, Karin and M{\"u}ller, Marina E.H. and Scherber, Christoph and Joshi, Jasmin Radha}, title = {Who is the culprit: Is pest infestation responsible for crop yield losses close to semi-natural habitats?}, series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1866-8372}, doi = {10.25932/publishup-54962}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-549622}, pages = {13232 -- 13246}, year = {2021}, abstract = {Semi-natural habitats (SNHs) are becoming increasingly scarce in modern agricultural landscapes. This may reduce natural ecosystem services such as pest control with its putatively positive effect on crop production. In agreement with other studies, we recently reported wheat yield reductions at field borders which were linked to the type of SNH and the distance to the border. In this experimental landscape-wide study, we asked whether these yield losses have a biotic origin while analyzing fungal seed and fungal leaf pathogens, herbivory of cereal leaf beetles, and weed cover as hypothesized mediators between SNHs and yield. We established experimental winter wheat plots of a single variety within conventionally managed wheat fields at fixed distances either to a hedgerow or to an in-field kettle hole. For each plot, we recorded the fungal infection rate on seeds, fungal infection and herbivory rates on leaves, and weed cover. Using several generalized linear mixed-effects models as well as a structural equation model, we tested the effects of SNHs at a field scale (SNH type and distance to SNH) and at a landscape scale (percentage and diversity of SNHs within a 1000-m radius). In the dry year of 2016, we detected one putative biotic culprit: Weed cover was negatively associated with yield values at a 1-m and 5-m distance from the field border with a SNH. None of the fungal and insect pests, however, significantly affected yield, neither solely nor depending on type of or distance to a SNH. However, the pest groups themselves responded differently to SNH at the field scale and at the landscape scale. Our findings highlight that crop losses at field borders may be caused by biotic culprits; however, their negative impact seems weak and is putatively reduced by conventional farming practices.}, language = {en} } @phdthesis{Itonaga2009, author = {Itonaga, Naomi}, title = {White storks (Ciconia ciconia) of Eastern Germany: age-dependent breeding ability, and age- and density-dependent effects on dispersal behavior}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-39052}, school = {Universit{\"a}t Potsdam}, year = {2009}, abstract = {Dispersal behavior plays an important role for the geographical distribution and population structure of any given species. Individual's fitness, reproductive and competitive ability, and dispersal behavior can be determined by the age of the individual. Age-dependent as well as density-dependent dispersal patterns are common in many bird species. In this thesis, I first present age-dependent breeding ability and natal site fidelity in white storks (Ciconia ciconia); migratory birds breeding in large parts of Europe. I predicted that both the proportion of breeding birds and natal site fidelity increase with the age. After the seventies of the last century, following a steep population decline, a recovery of the white stork population has been observed in many regions in Europe. Increasing population density in the white stork population in Eastern Germany especially after 1983 allowed examining density- as well as age-dependent breeding dispersal patterns. Therefore second, I present whether: young birds show more often and longer breeding dispersal than old birds, and frequency of dispersal events increase with the population density increase, especially in the young storks. Third, I present age- and density-dependent dispersal direction preferences in the give population. I asked whether and how the major spring migration direction interacts with dispersal directions of white storks: in different age, and under different population densities. The proportion of breeding individuals increased in the first 22 years of life and then decreased suggesting, the senescent decay in aging storks. Young storks were more faithful to their natal sites than old storks probably due to their innate migratory direction and distance. Young storks dispersed more frequently than old storks in general, but not for longer distance. Proportion of dispersing individuals increased significantly with increasing population densities indicating, density- dependent dispersal behavior in white storks. Moreover, the finding of a significant interaction effects between the age of dispersing birds and year (1980-2006) suggesting, older birds dispersed more from their previous nest sites over time due to increased competition. Both young and old storks dispersed along their spring migration direction; however, directional preferences were different in young storks and old storks. Young storks tended to settle down before reaching their previous nest sites (leading to the south-eastward dispersal) while old birds tended to keep migrating along the migration direction after reaching their previous nest sites (leading to the north-westward dispersal). Cues triggering dispersal events may be age-dependent. Changes in the dispersal direction over time were observed. Dispersal direction became obscured during the second half of the observation period (1993-2006). Increase in competition may affect dispersal behavior in storks. I discuss the potential role of: age for the observed age-dependent dispersal behavior, and competition for the density dependent dispersal behavior. This Ph.D. thesis contributes significantly to the understanding of population structure and geographical distribution of white storks. Moreover, presented age- and density (competition)-dependent dispersal behavior helps understanding underpinning mechanisms of dispersal behavior in bird species.}, language = {en} } @article{SchneidervanSchaikZangerleetal.2016, author = {Schneider, A. -K. and van Schaik, L. and Zangerle, A. and Eccard, Jana and Schroeder, B.}, title = {Which abiotic filters shape earthworm distribution patterns at the catchment scale?}, series = {European journal of soil science}, volume = {67}, journal = {European journal of soil science}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {1351-0754}, doi = {10.1111/ejss.12346}, pages = {431 -- 442}, year = {2016}, abstract = {Earthworms affect various soil ecosystem processes in their role as ecosystem engineers. The spatial distribution of earthworms determines the spatial distribution of their functional effects. In particular, earthworm-induced macropore networks may act as preferential flow pathways. In this research we aimed to determine earthworm distributions at the catchment scale with species distribution models (SDMs). We used land-use types, temporally invariant topography-related variables and plot-scale soil characteristics such as pH and organic matter content. We used data from spring 2013 to estimate probability distributions of the occurrence of ten earthworm species. To assess the robustness of these models, we tested temporal transferability by evaluating the accuracy of predictions from the models derived for the spring data with the predictions from data of two other field surveys in autumn 2012 and 2013. In addition, we compared the performance of SDMs based (i) on temporally varying plot-scale predictor variables with (ii) those based on temporally invariant catchment-scale predictors. Models based on catchment-scale predictors, especially land use and slope, experience a small loss of predictive performance only compared with plot-scale SDMs but have greater temporal transferability. Earthworm distribution maps derived from this kind of SDM are a prerequisite for understanding the spatial distribution patterns of functional effects related to earthworms.}, language = {en} } @article{PlueDeFrenneAcharyaetal.2017, author = {Plue, Jan and De Frenne, Pieter and Acharya, Kamal and Brunet, J{\"o}rg and Chabrerie, Olivier and Decocq, Guillaume and Diekmann, Martin and Graae, Bente J. and Heinken, Thilo and Hermy, Martin and Kolb, Annette and Lemke, Isgard and Liira, Jaan and Naaf, Tobias and Verheyen, Kris and Wulf, Monika and Cousins, Sara A. O.}, title = {Where does the community start, and where does it end?}, series = {Journal of vegetation science}, volume = {28}, journal = {Journal of vegetation science}, number = {2}, publisher = {Wiley}, address = {Hoboken}, issn = {1100-9233}, doi = {10.1111/jvs.12493}, pages = {424 -- 435}, year = {2017}, abstract = {QuestionBelow-ground processes are key determinants of above-ground plant population and community dynamics. Still, our understanding of how environmental drivers shape plant communities is mostly based on above-ground diversity patterns, bypassing below-ground plant diversity stored in seed banks. As seed banks may shape above-ground plant communities, we question whether concurrently analysing the above- and below-ground species assemblages may potentially enhance our understanding of community responses to environmental variation. LocationTemperate deciduous forests along a 2000km latitudinal gradient in NW Europe. MethodsHerb layer, seed bank and local environmental data including soil pH, canopy cover, forest cover continuity and time since last canopy disturbance were collected in 129 temperate deciduous forest plots. We quantified herb layer and seed bank diversity per plot and evaluated how environmental variation structured community diversity in the herb layer, seed bank and the combined herb layer-seed bank community. ResultsSeed banks consistently held more plant species than the herb layer. How local plot diversity was partitioned across the herb layer and seed bank was mediated by environmental variation in drivers serving as proxies of light availability. The herb layer and seed bank contained an ever smaller and ever larger share of local diversity, respectively, as both canopy cover and time since last canopy disturbance decreased. Species richness and -diversity of the combined herb layer-seed bank community responded distinctly differently compared to the separate assemblages in response to environmental variation in, e.g. forest cover continuity and canopy cover. ConclusionsThe seed bank is a below-ground diversity reservoir of the herbaceous forest community, which interacts with the herb layer, although constrained by environmental variation in e.g. light availability. The herb layer and seed bank co-exist as a single community by means of the so-called storage effect, resulting in distinct responses to environmental variation not necessarily recorded in the individual herb layer or seed bank assemblages. Thus, concurrently analysing above- and below-ground diversity will improve our ecological understanding of how understorey plant communities respond to environmental variation.}, language = {en} } @article{AlbertGrasseinSchurretal.2011, author = {Albert, C{\´e}cile H. and Grassein, Fabrice and Schurr, Frank Martin and Vieilledent, Ghislain and Violle, Cyrille}, title = {When and how should intraspecific variability be considered in trait-based plant ecology?}, series = {Perspectives in plant ecology, evolution and systematics}, volume = {13}, journal = {Perspectives in plant ecology, evolution and systematics}, number = {3}, publisher = {Elsevier}, address = {Jena}, issn = {1433-8319}, doi = {10.1016/j.ppees.2011.04.003}, pages = {217 -- 225}, year = {2011}, abstract = {Trait-based studies have become extremely common in plant ecology. Trait-based approaches often rely on the tacit assumption that intraspecific trait variability (ITV) is negligible compared to interspecific variability, so that species can be characterized by mean trait values. Yet, numerous recent studies have challenged this assumption by showing that ITV significantly affects various ecological processes. Accounting for ITV may thus strengthen trait-based approaches, but measuring trait values on a large number of individuals per species and site is not feasible. Therefore, it is important and timely to synthesize existing knowledge on ITV in order to (1) decide critically when ITV should be considered, and (2) establish methods for incorporating this variability. Here we propose a practical set of rules to identify circumstances under which ITV should be accounted for. We formulate a spatial trait variance partitioning hypothesis to highlight the spatial scales at which ITV cannot be ignored in ecological studies. We then refine a set of four consecutive questions on the research question, the spatial scale, the sampling design, and the type of studied traits, to determine case-by-case if a given study should quantify ITV and test its effects. We review methods for quantifying ITV and develop a step-by-step guideline to design and interpret simulation studies that test for the importance of ITV. Even in the absence of quantitative knowledge on ITV, its effects can be assessed by varying trait values within species within realistic bounds around the known mean values. We finish with a discussion of future requirements to further incorporate ITV within trait-based approaches. This paper thus delineates a general framework to account for ITV and suggests a direction towards a more quantitative trait-based ecology.}, language = {en} } @misc{BeckBallesterosMejiaBuchmannetal.2012, author = {Beck, Jan and Ballesteros-Mejia, Liliana and Buchmann, Carsten M. and Dengler, J{\"u}rgen and Fritz, Susanne A. and Gruber, Bernd and Hof, Christian and Jansen, Florian and Knapp, Sonja and Kreft, Holger and Schneider, Anne-Kathrin and Winter, Marten and Dormann, Carsten F.}, title = {What's on the horizon for macroecology?}, series = {Ecography : pattern and diversity in ecology ; research papers forum}, volume = {35}, journal = {Ecography : pattern and diversity in ecology ; research papers forum}, number = {8}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {0906-7590}, doi = {10.1111/j.1600-0587.2012.07364.x}, pages = {673 -- 683}, year = {2012}, abstract = {Over the last two decades, macroecology the analysis of large-scale, multi-species ecological patterns and processes has established itself as a major line of biological research. Analyses of statistical links between environmental variables and biotic responses have long and successfully been employed as a main approach, but new developments are due to be utilized. Scanning the horizon of macroecology, we identified four challenges that will probably play a major role in the future. We support our claims by examples and bibliographic analyses. 1) Integrating the past into macroecological analyses, e.g. by using paleontological or phylogenetic information or by applying methods from historical biogeography, will sharpen our understanding of the underlying reasons for contemporary patterns. 2) Explicit consideration of the local processes that lead to the observed larger-scale patterns is necessary to understand the fine-grain variability found in nature, and will enable better prediction of future patterns (e.g. under environmental change conditions). 3) Macroecology is dependent on large-scale, high quality data from a broad spectrum of taxa and regions. More available data sources need to be tapped and new, small-grain large-extent data need to be collected. 4) Although macroecology already lead to mainstreaming cutting-edge statistical analysis techniques, we find that more sophisticated methods are needed to account for the biases inherent to sampling at large scale. Bayesian methods may be particularly suitable to address these challenges. To continue the vigorous development of the macroecological research agenda, it is time to address these challenges and to avoid becoming too complacent with current achievements.}, language = {en} } @article{DreyerBlatt2009, author = {Dreyer, Ingo and Blatt, Michael R.}, title = {What makes a gate? : the ins and outs of Kv-like K+ channels in plants}, issn = {1360-1385}, doi = {10.1016/j.tplants.2009.04.001}, year = {2009}, abstract = {Gating of K+ and other ion channels is 'hard-wired' within the channel protein. So it remains a puzzle how closely related channels in plants can show an unusually diverse range of biophysical properties. Gating of these channels lies at the heart of K+ mineral nutrition, signalling, abiotic and biotic stress responses in plants. Thus, our knowledge of the molecular mechanics underpinning K+ channel gating will be important for rational engineering of related traits in agricultural crops. Several key studies have added significantly to our understanding of channel gating in plants and have challenged current thinking about analogous processes found in animal K+ channels. Such studies highlight how much of K+ channel gating remains to be explored in plants.}, language = {en} } @article{BergerWaltersGotthard2008, author = {Berger, Dieter and Walters, R. J. and Gotthard, K.}, title = {What limits insect fecundity? : Body size- and temperature-dependent egg maturation and oviposition in a butterfly}, issn = {0269-8463}, doi = {10.1111/j.1365-2435.2008.01392.x}, year = {2008}, abstract = {* 1. Large female insects usually have high potential fecundity. Therefore selection should favour an increase in body size given that these females get opportunities to realize their potential advantage by maturing and laying more eggs. However, ectotherm physiology is strongly temperature-dependent, and activities are carried out sufficiently only within certain temperature ranges. Thus it remains unclear if the fecundity advantage of a large size is fully realized in natural environments, where thermal conditions are limiting. * 2. Insect fecundity might be limited by temperature at two levels; first eggs need to mature, and then the female needs time for strategic ovipositing of the egg. Since a female cannot foresee the number of oviposition opportunities that she will encounter on a given day, the optimal rate of egg maturation will be governed by trade-offs associated with egg- and time-limited oviposition. As females of different sizes will have different amounts of body reserves, size-dependent allocation trade-offs between the mother"s condition and her egg production might be expected. * 3. In the temperate butterfly Pararge aegeria, the time and temperature dependence of oviposition and egg maturation, and the interrelatedness of these two processes were investigated in a series of laboratory experiments, allowing a decoupling of the time budgets for the respective processes. * 4. The results show that realized fecundity of this species can be limited by both the temperature dependence of egg maturation and oviposition under certain thermal regimes. Furthermore, rates of oviposition and egg maturation seemed to have regulatory effects upon each other. Early reproductive output was correlated with short life span, indicating a cost of reproduction. Finally, large females matured more eggs than small females when deprived of oviposition opportunities. Thus, the optimal allocation of resources to egg production seems dependent on female size. * 5. This study highlights the complexity of processes underlying rates of egg maturation and oviposition in ectotherms under natural conditions. We further discuss the importance of temperature variation for egg- vs. time-limited fecundity and the consequences for the evolution of female body size in insects.}, language = {en} } @article{LehmannScheffler2016, author = {Lehmann, Andreas and Scheffler, Christiane}, title = {What does the mean menarcheal age mean?An analysis of temporal pattern in variability in a historical swiss population from the 19th and 20th centuries}, series = {American journal of human biology : the official journal of the Human Biology Council}, volume = {28}, journal = {American journal of human biology : the official journal of the Human Biology Council}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {1042-0533}, doi = {10.1002/ajhb.22854}, pages = {705 -- 713}, year = {2016}, abstract = {ObjectivesAge at menarche is one of the most important factors when observing growth and development. The aim of this study was to assess the temporal pattern in variability of menarcheal age for a historic Swiss population from the 19th and 20th centuries. ResultsMean menarcheal age declined from 17.34 years (n=358) around 1830 to 13.80 years (n=141) around 1950. Within-cohort variance decreased from 7.5 to 2.1 year(2). Skewness was negatively correlated with birth year (r=-0.58). ConclusionThis study provided evidence for a secular trend in various statistical parameters for age at menarche since the 19th century. Furthermore, the results of the analysis of temporal pattern in variability revealed that the secular trend in menarcheal age happened in two phases. Am. J. Hum. Biol. 28:705-713, 2016. (c) 2016 Wiley Periodicals, Inc.}, language = {en} } @article{SchefflerHermanussen2023, author = {Scheffler, Christiane and Hermanussen, Michael}, title = {What does stunting tell us?}, series = {Human biology and public health}, volume = {2022}, journal = {Human biology and public health}, number = {3}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {2748-9957}, doi = {10.52905/hbph2022.3.36}, pages = {1 -- 15}, year = {2023}, abstract = {Stunting is commonly linked with undernutrition. Yet, already after World War I, German pediatricians questioned this link and stated that no association exists between nutrition and height. Recent analyses within different populations of Low- and middle-income countries with high rates of stunted children failed to support the assumption that stunted children have a low BMI and skinfold sickness as signs of severe caloric deficiency. So, stunting is not a synonym of malnutrition. Parental education level has a positive influence on body height in stunted populations, e.g., in India and in Indonesia. Socially disadvantaged children tend to be shorter and lighter than children from affluent families. Humans are social mammals; they regulate growth similar to other social mammals. Also in humans, body height is strongly associated with the position within the social hierarchy, reflecting the personal and group-specific social, economic, political, and emotional environment. These non-nutritional impact factors on growth are summarized by the concept of SEPE (Social-Economic-Political-Emotional) factors. SEPE reflects on prestige, dominance-subordination, social identity, and ego motivation of individuals and social groups.}, language = {en} } @article{ChorusSpijkerman2020, author = {Chorus, Ingrid and Spijkerman, Elly}, title = {What Colin Reynolds could tell us about nutrient limitation, N:P ratios and eutrophication control}, series = {Hydrobiologia : acta hydrobiologica, hydrographica, limnologica et protistologica}, volume = {848}, journal = {Hydrobiologia : acta hydrobiologica, hydrographica, limnologica et protistologica}, number = {1}, publisher = {Springer Nature}, address = {Berlin}, issn = {0018-8158}, doi = {10.1007/s10750-020-04377-w}, pages = {95 -- 111}, year = {2020}, abstract = {Colin Reynolds exquisitely consolidated our understanding of driving forces shaping phytoplankton communities and those setting the upper limit to biomass yield, with limitation typically shifting from light in winter to phosphorus in spring. Nonetheless, co-limitation is frequently postulated from enhanced growth responses to enrichments with both N and P or from N:P ranging around the Redfield ratio, concluding a need to reduce both N and P in order to mitigate eutrophication. Here, we review the current understanding of limitation through N and P and of co-limitation. We conclude that Reynolds is still correct: (i) Liebig's law of the minimum holds and reducing P is sufficient, provided concentrations achieved are low enough; (ii) analyses of nutrient limitation need to exclude evidently non-limiting situations, i.e. where soluble P exceeds 3-10 mu g/l, dissolved N exceeds 100-130 mu g/l and total P and N support high biomass levels with self-shading causing light limitation; (iii) additionally decreasing N to limiting concentrations may be useful in specific situations (e.g. shallow waterbodies with high internal P and pronounced denitrification); (iv) management decisions require local, situation-specific assessments. The value of research on stoichiometry and co-limitation lies in promoting our understanding of phytoplankton ecophysiology and community ecology.}, language = {en} } @misc{ChorusSpijkerman2020, author = {Chorus, Ingrid and Spijkerman, Elly}, title = {What Colin Reynolds could tell us about nutrient limitation, N:P ratios and eutrophication control}, series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {1}, issn = {1866-8372}, doi = {10.25932/publishup-54197}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-541979}, pages = {19}, year = {2020}, abstract = {Colin Reynolds exquisitely consolidated our understanding of driving forces shaping phytoplankton communities and those setting the upper limit to biomass yield, with limitation typically shifting from light in winter to phosphorus in spring. Nonetheless, co-limitation is frequently postulated from enhanced growth responses to enrichments with both N and P or from N:P ranging around the Redfield ratio, concluding a need to reduce both N and P in order to mitigate eutrophication. Here, we review the current understanding of limitation through N and P and of co-limitation. We conclude that Reynolds is still correct: (i) Liebig's law of the minimum holds and reducing P is sufficient, provided concentrations achieved are low enough; (ii) analyses of nutrient limitation need to exclude evidently non-limiting situations, i.e. where soluble P exceeds 3-10 mu g/l, dissolved N exceeds 100-130 mu g/l and total P and N support high biomass levels with self-shading causing light limitation; (iii) additionally decreasing N to limiting concentrations may be useful in specific situations (e.g. shallow waterbodies with high internal P and pronounced denitrification); (iv) management decisions require local, situation-specific assessments. The value of research on stoichiometry and co-limitation lies in promoting our understanding of phytoplankton ecophysiology and community ecology.}, language = {en} } @phdthesis{CamaraMattosMartins2011, author = {Camara Mattos Martins, Marina}, title = {What are the downstream targets of trehalose-6-phosphate signalling in plants?}, address = {Potsdam}, pages = {164 S.}, year = {2011}, language = {en} } @misc{BoekerHermanussenScheffler2021, author = {Boeker, Sonja and Hermanussen, Michael and Scheffler, Christiane}, title = {Westernization of self-perception in modern affluent Indonesian school children}, series = {Human Biology and Public Health}, volume = {2021}, journal = {Human Biology and Public Health}, number = {1}, editor = {Scheffler, Christiane and Koziel, Slawomir and Hermanussen, Michael and Bogin, Barry}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {2748-9957}, doi = {10.52905/hbph.v1.4}, pages = {1 -- 13}, year = {2021}, abstract = {Background Subjective Social Status is used as an important predictor for psychological and physiological findings, most commonly measured with the MacArthur Scale (Ladder Test). Previous studies have shown that this test fits better in Western cultures. The idea of a social ladder itself and ranking oneself "higher" or "lower" is a concept that accords to the Western thinking. Objectives We hypothesize that in a culture where only the elites have adapted to a Western lifestyle, the test results reflect a higher level of accuracy for this stratum. We also expect that self-perception differs per sex. Sample and Methods We implemented the Ladder Test in a study of Indonesian schoolchildren aged between 5 and 13 years (boys N = 369, girls N= 364) from non-private and private schools in Kupang in 2020. Results Our analysis showed that the Ladder Test results were according to the Western expectations only for the private school, as the Ladder Scores significantly decreased with age (LM: p = 0.04). The Ladder Test results are best explained by "Education Father" for the non-private school pupils (p = 0.01) and all boys (p = 0.04), by "School Grades" for the private school cohort (p = 0.06) and by "Household Score" for girls (p =0.09). Conclusion This finding indicates that the concept of ranking oneself "high" or "low" on a social ladder is strongly implicated with Western ideas. A ladder implies social movement by "climbing" up or down. According to that, reflection of self-perception is influenced by culture.}, language = {en} } @article{Martens2011, author = {Martens, D{\"o}rte}, title = {Well-being and acceptance - contradictory aims in forest management?}, series = {Eco.mont : journal on protected mountain areas research}, volume = {3}, journal = {Eco.mont : journal on protected mountain areas research}, number = {2}, publisher = {Austrian Academy of Sciences Press}, address = {Wien}, issn = {2073-106X}, pages = {63 -- 65}, year = {2011}, abstract = {Urban forests fulfil various functions, among them the restoration process and aesthetical needs of urban residents. This article reflects the attitudes towards different managed forests on the one hand and their influence on psychological well-being on the other. Results of empirical approaches from both fields show some inconsistency, suggesting that people have a more positive attitude towards wild forest areas, while the effect on well-being is more positive after a walk in tended forest areas. A discussion follows on the link between perception and the effect of urban forests. An outlook on necessary research reveals the need for longitudinal research. The article concludes by showing management implications.}, language = {en} } @article{HoffmannHoelkerEccard2022, author = {Hoffmann, Julia and H{\"o}lker, Franz and Eccard, Jana}, title = {Welcome to the dark side}, series = {Frontiers in ecology and evolution}, volume = {9}, journal = {Frontiers in ecology and evolution}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-701X}, doi = {10.3389/fevo.2021.779825}, pages = {11}, year = {2022}, abstract = {Differences in natural light conditions caused by changes in moonlight are known to affect perceived predation risk in many nocturnal prey species. As artificial light at night (ALAN) is steadily increasing in space and intensity, it has the potential to change movement and foraging behavior of many species as it might increase perceived predation risk and mask natural light cycles. We investigated if partial nighttime illumination leads to changes in foraging behavior during the night and the subsequent day in a small mammal and whether these changes are related to animal personalities. We subjected bank voles to partial nighttime illumination in a foraging landscape under laboratory conditions and in large grassland enclosures under near natural conditions. We measured giving-up density of food in illuminated and dark artificial seed patches and video recorded the movement of animals. While animals reduced number of visits to illuminated seed patches at night, they increased visits to these patches at the following day compared to dark seed patches. Overall, bold individuals had lower giving-up densities than shy individuals but this difference increased at day in formerly illuminated seed patches. Small mammals thus showed carry-over effects on daytime foraging behavior due to ALAN, i.e., nocturnal illumination has the potential to affect intra- and interspecific interactions during both night and day with possible changes in personality structure within populations and altered predator-prey dynamics.}, language = {en} } @misc{HoffmannHoelkerEccard2022, author = {Hoffmann, Julia and H{\"o}lker, Franz and Eccard, Jana}, title = {Welcome to the Dark Side}, series = {Postprints der Universit{\"a}t Potsdam Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam Mathematisch-Naturwissenschaftliche Reihe}, issn = {1866-8372}, doi = {10.25932/publishup-54470}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-544702}, pages = {13}, year = {2022}, abstract = {Differences in natural light conditions caused by changes in moonlight are known to affect perceived predation risk in many nocturnal prey species. As artificial light at night (ALAN) is steadily increasing in space and intensity, it has the potential to change movement and foraging behavior of many species as it might increase perceived predation risk and mask natural light cycles. We investigated if partial nighttime illumination leads to changes in foraging behavior during the night and the subsequent day in a small mammal and whether these changes are related to animal personalities. We subjected bank voles to partial nighttime illumination in a foraging landscape under laboratory conditions and in large grassland enclosures under near natural conditions. We measured giving-up density of food in illuminated and dark artificial seed patches and video recorded the movement of animals. While animals reduced number of visits to illuminated seed patches at night, they increased visits to these patches at the following day compared to dark seed patches. Overall, bold individuals had lower giving-up densities than shy individuals but this difference increased at day in formerly illuminated seed patches. Small mammals thus showed carry-over effects on daytime foraging behavior due to ALAN, i.e., nocturnal illumination has the potential to affect intra- and interspecific interactions during both night and day with possible changes in personality structure within populations and altered predator-prey dynamics.}, language = {en} } @article{HoffmannHoelkerEccard2022, author = {Hoffmann, Julia and H{\"o}lker, Franz and Eccard, Jana}, title = {Welcome to the Dark Side}, series = {Frontiers in Ecology and Evolution}, volume = {9}, journal = {Frontiers in Ecology and Evolution}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-701X}, doi = {10.3389/fevo.2021.779825}, pages = {11}, year = {2022}, abstract = {Differences in natural light conditions caused by changes in moonlight are known to affect perceived predation risk in many nocturnal prey species. As artificial light at night (ALAN) is steadily increasing in space and intensity, it has the potential to change movement and foraging behavior of many species as it might increase perceived predation risk and mask natural light cycles. We investigated if partial nighttime illumination leads to changes in foraging behavior during the night and the subsequent day in a small mammal and whether these changes are related to animal personalities. We subjected bank voles to partial nighttime illumination in a foraging landscape under laboratory conditions and in large grassland enclosures under near natural conditions. We measured giving-up density of food in illuminated and dark artificial seed patches and video recorded the movement of animals. While animals reduced number of visits to illuminated seed patches at night, they increased visits to these patches at the following day compared to dark seed patches. Overall, bold individuals had lower giving-up densities than shy individuals but this difference increased at day in formerly illuminated seed patches. Small mammals thus showed carry-over effects on daytime foraging behavior due to ALAN, i.e., nocturnal illumination has the potential to affect intra- and interspecific interactions during both night and day with possible changes in personality structure within populations and altered predator-prey dynamics.}, language = {en} } @article{Heinken2008, author = {Heinken, Thilo}, title = {Welche populationsbiologischen und genetischen Konsequenzen hat Habitatfragmentierung f{\"u}r Pflanzen? : Wissenschaftliche Grundlagen f{\"u}r ein Biotopverbundsystem f{\"u}r Pflanzen in Brandenburg}, issn = {0942-9328}, year = {2008}, abstract = {Neben dem Habitatverlust gelten Konsequenzen der Habitatfragmentierung seit den 1990er Jahren als wesentliche Ursache der Gefaehrdung von Pflanzen und stehen damit nun auch im Fokus des botanischen Artenschutzes. Der vorliegende Beitrag gibt einen ueberblick ueber den Stand der populationsbiologischen und genetischen Forschung und versucht abzuschaetzen, welche Bedeutung Habitatfragmentierung und die dadurch entstehenden kleinen, isolierten Populationen auf heimische Pflanzenarten haben koennen. Als wesentliche und offenbar sehr weit verbreitete negative Effekte werden Zufallsereignisse, Randeffekte, Bestaeuberlimitierung, Gendrift und Inzuchtdepression identifiziert. Zusammen mit verringerter Habitatqualitaet durch Eutrophierung, Entwaesserung oder Nutzungsaenderung wirken sie zumeist negativ auf die Fitness der Individuen und Populationen und erhoehen so deren Aussterberisiko. Dieser negative Effekt kleiner Populationen auf die individuelle Fitness wird unabhaengig von der Ursache als Allee-Effekt bezeichnet. Eine durch einen Biotopverbund gefoerderte Metapopulationsdynamik kann das dauerhafte Aussterben von Pflanzenpopulationen verhindern und mindert die negativen genetischen Effekte der Habitatfragmentierung ueber einen erhoehten Genfluss durch Pollen und Samen. Die bisherigen wissenschaftlichen Studien in Mitteleuropa beruhen allerdings in ueberproportionaler Weise auf bestimmten Pflanzenfamilien (Gentianaceae, Primulaceae), Habitaten (Trocken- und Magerrasen, Wirtschaftsgruenland), insekten- und obligat fremdbestaeubten sowie weitgehend auf sexuelle Fortpflanzung angewiesenen Arten, waehrend etwa ueber Grasartige, Ruderalpflanzen, wind- und selbstbestaeubte sowie an vegetative Fortpflanzung angepasste Arten nur wenige Erkenntnisse vorliegen. Gerade diese und Pflanzenarten mit hohem Ausbreitungspotenzial muessen aber nach derzeitigem Wissensstand als weniger sensitiv gegenueber Habitatfragmentierung eingestuft werden. Auf diesen Befunden aufbauend werden fuer die Naturschutzpraxis Biotoptypen hinsichtlich ihrer Sensitivitaet gegenueber Habitatfragmentierung klassifiziert und ein auf biologischen Merkmalen basierender Kriterienkatalog zur Auswahl von Zielarten des Biotopverbunds vorgestellt. Schließlich wird eroertert, was bei Maßnahmen zur Regeneration kleiner bzw. bereits ausgestorbener Populationen zu beachten ist, und es werden allgemeine Folgerungen zur Ausgestaltung eines Biotopverbundskonzepts fuer Pflanzen gezogen.}, language = {de} } @phdthesis{Pitzen2022, author = {Pitzen, Valentin}, title = {Weitergef{\"u}hrte funktionelle Charakterisierung des centrosomalen Proteins Cep192 und Untersuchung der Topologie des Centrosoms in Dictyostelium Am{\"o}ben}, doi = {10.25932/publishup-54889}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-548891}, school = {Universit{\"a}t Potsdam}, pages = {XI, 104}, year = {2022}, abstract = {Das Centrosom von Dictyostelium ist acentriol{\"a}r aufgebaut, misst ca. 500 nm und besteht aus einer dreischichten Core-Struktur mit umgebender Corona, an der Mikrotubuli nukleieren. In dieser Arbeit wurden das centrosomale Protein Cep192 und m{\"o}gliche Interaktionspartner am Centrosom eingehend untersucht. Die einleitende Lokalisationsuntersuchung von Cep192 ergab, dass es w{\"a}hrend der gesamten Mitose an den Spindelpolen lokalisiert und im Vergleich zu den anderen Strukturproteinen der Core-Struktur am st{\"a}rksten exprimiert ist. Die dauerhafte Lokalisation an den Spindelpolen w{\"a}hrend der Mitose wird f{\"u}r Proteine angenommen, die in den beiden identisch aufgebauten {\"a}ußeren Core-Schichten lokalisieren, die das mitotische Centrosom formen. Ein Knockdown von Cep192 f{\"u}hrte zur Ausbildung von {\"u}berz{\"a}hligen Mikrotubuli-organisierenden Zentren (MTOC) sowie zu einer leicht erh{\"o}hten Ploidie. Deshalb wird eine Destabilisierung des Centrosoms durch die verminderte Cep192-Expression angenommen. An Cep192 wurden zwei kleine Tags, der SpotH6- und BioH6-Tag, etabliert, die mit kleinen fluoreszierenden Nachweiskonjugaten markiert werden konnten. Mit den so getagten Proteinen konnte die hochaufl{\"o}sende Expansion Microscopy f{\"u}r das Centrosom optimiert werden und die Core-Struktur erstmals proteinspezifisch in der Fluoreszenzmikroskopie dargestellt werden. Cep192 lokalisiert dabei in den {\"a}ußeren Core-Schichten. Die kombinierte Markierung von Cep192 und den centrosomalen Proteinen CP39 und CP91 in der Expansion Microscopy erlaubte die Darstellung des dreischichtigen Aufbaus der centrosomalen Core-Struktur, wobei CP39 und CP91 zwischen Cep192 in der inneren Core-Schicht lokalisieren. Auch die Corona wurde in der Expansion Microscopy untersucht: Das Corona-Protein CDK5RAP2 lokalisiert in r{\"a}umlicher N{\"a}he zu Cep192 in der inneren Corona. Ein Vergleich der Corona-Proteine CDK5RAP2, CP148 und CP224 in der Expansion Microscopy ergab unterscheidbare Sublokalisationen der Proteine innerhalb der Corona und relativ zur Core-Struktur. In Biotinylierungsassays mit den centrosomalen Core-Proteinen CP39 und CP91 sowie des Corona-Proteins CDK5RAP2 konnte Cep192 als m{\"o}glicher Interaktionspartner identifiziert werden. Die Ergebnisse dieser Arbeit zeigen die wichtige Funktion des Proteins Cep192 im Dictyostelium-Centrosom und erm{\"o}glichen durch die Kombination aus Biotinylierungsassays und Expansion Microscopy der untersuchten Proteine ein verbessertes Verst{\"a}ndnis der Topologie des Centrosoms.}, language = {de} } @article{AppelhagenHuepLuetal.2010, author = {Appelhagen, Ingo and Huep, Gunnar and Lu, Gui-Hua and Strompen, Georg and Weisshaar, Bernd and Sagasser, Martin}, title = {Weird fingers : functional analysis of WIP domain proteins}, issn = {0014-5793}, doi = {10.1016/j.febslet.2010.06.007}, year = {2010}, abstract = {WIP proteins form a plant specific subfamily of C2H2 zinc finger (ZF) proteins. In this study, we functionally characterized the WIP domain, which consists of four ZF motifs, and discuss molecular functions for WIP proteins. Mutations in each of the ZFs lead to loss of function of the TT1/WIP1 protein in Arabiopsis thaliana. SV40 type nuclear localisation signals were detected in two of the ZFs and functionally characterized using GFP fusions as well as new mutant alleles identified by TILLING. Promoter swap experiments showed that selected WIP proteins are partially able to take over TT1 function. Activity of the AtBAN promoter, a potential TT1 target, could be increased by the addition of TT1 to the TT2-TT8-TTG1 regulatory complex.}, language = {en} } @article{HermanussenBilogubLindletal.2018, author = {Hermanussen, Michael and Bilogub, Maria and Lindl, A. C. and Harper, D. and Mansukoski, L. and Scheffler, Christiane}, title = {Weight and height growth of malnourished school-age children during re-feeding}, series = {European journal of clinical nutrition}, volume = {72}, journal = {European journal of clinical nutrition}, number = {12}, publisher = {Nature Publ. Group}, address = {London}, issn = {0954-3007}, doi = {10.1038/s41430-018-0274-z}, pages = {1603 -- 1619}, year = {2018}, abstract = {Background In view of the ongoing debate on "chronic malnutrition" and the concept of "stunting" as "a better measure than underweight of the cumulative effects of undernutrition and infection (WHO)", we translate, briefly comment and republish three seminal historic papers on catch-up growth following re-feeding after severe food restriction of German children during and after World War I. The observations were published in 1920 and 1922, and appear to be of particular interest to the modern nutritionist. Results The papers of Abderhalden (1920) and Bloch (1920) describe German children of all social strata who were born shortly before World War I, and raised in apparently "normal" families. After severe long-standing undernutrition, they participated in an international charity program. They experienced exceptional catch-up growth in height of 3-5 cm within 6-8 weeks. Goldstein (1922) observed 512 orphans and children from underprivileged families. Goldstein described very different growth patterns. These children were much shorter (mean height between -2.0 and -2.8 SDS, modern WHO reference). They mostly failed to catch-up in height, but tended to excessively increase in weight particularly during adolescence.}, language = {en} } @phdthesis{Mofina2012, author = {Mofina, Sabine}, title = {Weighing the Role of the Hypoxia Inducible Factor-1a in Tumor and Stroma for tumorigenesis}, address = {Potsdam}, pages = {IX, 132 S.}, year = {2012}, language = {en} } @book{Scharf1994, author = {Scharf, Peter}, title = {Wegweiser durch die Gew{\"a}chsh{\"a}user und Freilandanlagen des Botanischen Gartens der Universit{\"a}t Potsdam}, publisher = {Univ.}, address = {Potsdam}, pages = {64 S.}, year = {1994}, language = {de} } @article{KummerRichterSchwik2005, author = {Kummer, Volker and Richter, Torsten and Schwik, J{\"u}rgen}, title = {Wegelina grumsiniana comb. nov. (Ascomycetes, Calosphaeriales) : ein Pyrenomycet auf der Porenschicht faulender Zunderschw{\"a}mme (Fomes fomentarius)}, year = {2005}, language = {de} } @phdthesis{Edner2008, author = {Edner, Christoph}, title = {Wechselwirkungen zwischen Glucan, Wasser-Dikinase (GWD) und Glucan-Hydrolasen beim Abbau transitorischer Balttst{\"a}rke}, address = {Potsdam}, pages = {vi, 107 S. : graph. Darst.}, year = {2008}, language = {de} } @phdthesis{Heilmann2006, author = {Heilmann, Katja}, title = {Wechselwirkungen von Immunzellen mit synthetischen und biomimetischen Oberfl{\"a}chen}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-8843}, school = {Universit{\"a}t Potsdam}, year = {2006}, abstract = {Die vorliegende Arbeit wurde im Zeitraum von Oktober 2002 bis November 2005 an dem Institut f{\"u}r Biochemie und Biologie der Universit{\"a}t Potsdam in Kooperation mit dem Institut f{\"u}r Chemie des GKSS Forschungszentrums in Teltow unter der Leitung von Herrn Prof. Dr. B. Micheel und Herrn Prof. Dr. Th. Groth angefertigt. Im Rahmen dieser Arbeit wurden die Wechselwirkungen von Immunzellen mit verschiedenen Kultursubstraten untersucht. Daf{\"u}r wurden drei verschiedene Hybridomzelllinien eingesetzt. Eine Hybridomzelllinie (K2) ist im Laufe dieser Arbeit hergestellt und etabliert worden. Der Einsatz von synthetischen und proteinbeschichteten Kulturoberfl{\"a}chen f{\"u}hrte bei Hybridomzellen zu einer deutlich gesteigerten Antik{\"o}rpersynthese im Vergleich zu herk{\"o}mmlichen Zellkulturmaterialien. Obwohl diese Zellen in der Regel als Suspensionszellen kultiviert werden, f{\"u}hrten die eingesetzten Polymermembranen (PAN, NVP) zu einer verbesserten Antik{\"o}rpersynthese (um 30\%) gegen{\"u}ber Polystyrol als Referenz. Es konnte gezeigt werden, dass es einen Zusammenhang zwischen der Produktivit{\"a}t und dem Adh asionsverhalten der Hybridomzellen gibt. Um den Einfluss von Proteinen der extrazellul{\"a}ren Matrix auf Zellwachstum und Antik{\"o}rpersynthese von Hybridomzellen zu untersuchen, wurden proteinbeschichtete Polystyrol-Oberfl{\"a}chen eingesetzt. F{\"u}r die Modifikationen wurden Fibronektin, Kollagen I, Laminin und BSA ausgew{\"a}hlt. Die Modifikation der Polystyrol-Oberfl{\"a}che mit geringen Mengen Fibronektin (0,2-0,4 µg/ml) f{\"u}hrte zu einer betr{\"a}chtlichen Steigerung der Antik{\"o}rpersynthese um 70-120\%. F{\"u}r Kollagen I- und BSA-Beschichtungen konnten Steigerungen von 40\% beobachtet werden. Modifikationen der Polystyrol-Oberfl{\"a}che mit Laminin zeigten nur marginale Effekte. Durch weitere Versuche wurde best{\"a}tigt, dass die Adh{\"a}sion der Zellen an Kollagen I- und Laminin-beschichteten Oberfl{\"a}chen verringert ist. Die alpha2-Kette des alpha2beta1-Integrins konnte auf der Zelloberfl{\"a}che nicht nachgewiesen werden. Durch ihr Fehlen wird wahrscheinlich die Bindungsf{\"a}higkeit der Zellen an Kollagen I und Laminin beeinflusst. Durch die Ergebnisse konnte gezeigt werden, dass Hybridomzellen nicht nur Suspensionszellen sind und das Kultursubstrate das Zellwachstum und die Produktivit{\"a}t dieser Zellen stark beeinflussen k{\"o}nnen. Der Einsatz von synthetischen und proteinbeschichteten Kultursubstraten zur Steigerung der Antik{\"o}rpersynthese kann damit f{\"u}r die industrielle Anwendung von großer Relevanz sein. F{\"u}r die Modellierung einer Lymphknotenmatrix wurden Fibronektin, Kollagen I, Heparansulfat und N-Acetylglucosamin-mannose in verschiedenen Kombinationen an Glasoberfl{\"a}chen adsorbiert und f{\"u}r Versuche zur In-vitro-Immunisierung eingesetzt. Es konnte gezeigt werden, dass die Modifikation der Oberfl{\"a}chen die Aktivierung und Interaktion von dendritischen Zellen, T- und B-Lymphozyten beg{\"u}nstigt, was durch den Nachweis spezifischer Interleukine (IL12, IL6) und durch die Synthese spezifischer Antik{\"o}rper best{\"a}tigt wurde. Eine spezifische Immunreaktion gegen das Antigen Ovalbumin konnte mit den eingesetzten Zellpopulationen aus Ovalbumin-T-Zell-Rezeptor-transgenen M{\"a}usen nachgewiesen werden. Die In-vitro-Immunantwort wurde dabei am st{\"a}rksten durch eine Kombination von Kollagen I, Heparansulfat und N-Acetylglucosamin-mannose auf einer Glasoberfl{\"a}che gef{\"o}rdert. Die Etablierung einer k{\"u}nstlichen Immunreaktion kann eine gesteuerte Aktivierung bzw. Inaktivierung von k{\"o}rpereigenen dendritischen Zellen gegen bestehende Krankheitsmerkmale in vitro erm{\"o}glichen. Durch die Versuche wurden Grundlagen f{\"u}r spezifische Immunantworten erarbeitet, die u.a. f{\"u}r die Herstellung von humanen Antik{\"o}rpern eingesetzt werden k{\"o}nnen.}, subject = {Hybridomtechnik}, language = {de} } @phdthesis{Gromelski2006, author = {Gromelski, Sandra}, title = {Wechselwirkung zwischen Lipiden und DNA : auf dem Weg zum k{\"u}nstlichen Virus}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-7629}, school = {Universit{\"a}t Potsdam}, year = {2006}, abstract = {Weltweit versuchen Wissenschaftler, k{\"u}nstliche Viren f{\"u}r den Gentransfer zu konstruieren, die nicht reproduktionsf{\"a}hig sind. Diese sollen die Vorteile der nat{\"u}rlichen Viren besitzen (effizienter Transport von genetischem Material), jedoch keine Antigene auf ihrer Oberfl{\"a}che tragen, die Immunreaktionen ausl{\"o}sen. Ziel dieses Projektes ist es, einen k{\"u}nstlichen Viruspartikel herzustellen, dessen Basis eine Polyelektrolytenhohlkugel bildet, die mit einer Lipiddoppelschicht bedeckt ist. Um intakte Doppelschichten zu erzeugen, muss die Wechselwirkung zwischen Lipid und Polyelektrolyt (z.B. DNA) verstanden und optimiert werden. Dazu ist es notwendig, die strukturelle Grundlage der Interaktion aufzukl{\"a}ren. Positiv geladene Lipide gehen zwar starke Wechselwirkungen mit der negativ geladenen DNA ein, sie wirken jedoch toxisch auf biologische Zellen. In der vorliegenden Arbeit wurde daher die durch zweiwertige Kationen vermittelte Kopplung von genomischer oder Plasmid-DNA an zwitterionische oder negativ geladene Phospholipide an zwei Modellsystemen untersucht. 1. Modellsystem: Lipidmonoschicht an der Wasser/Luft-Grenzfl{\"a}che Methoden: Filmwaagentechnik in Kombination mit IR-Spektroskopie (IRRAS), R{\"o}ntgenreflexion (XR), R{\"o}ntgendiffraktion (GIXD), Brewsterwinkel-Mikroskopie (BAM), R{\"o}ntgenfluoreszenz (XRF) und Oberfl{\"a}chenpotentialmessungen Resultate: A) Die Anwesenheit der zweiwertigen Kationen Ba2+, Mg2+, Ca2+ oder Mn2+ in der Subphase hat keinen nachweisbaren Einfluss auf die Struktur der zwitterionischen DMPE- (1,2-Dimyristoyl-phosphatidyl-ethanolamin) Monoschicht. B) In der Subphase gel{\"o}ste DNA adsorbiert nur in Gegenwart dieser Kationen an der DMPE-Monoschicht. C) Sowohl die Adsorption genomischer Kalbsthymus-DNA als auch der Plasmid-DNA pGL3 bewirkt eine Reduktion des Neigungswinkels der Alkylketten, die auf einen ver{\"a}nderten Platzbedarf der Kopfgruppe zur{\"u}ckzuf{\"u}hren ist. Durch die Umorientierung der Kopfgruppe wird die elektrostatische Wechselwirkung zwischen den positiv geladenen Stickstoffatomen der Lipidkopfgruppen und den negativ geladenen DNA-Phosphaten erh{\"o}ht. D) Die adsorbierte DNA weist eine geordnete Struktur auf, wenn sie durch Barium-, Magnesium-, Calcium- oder Manganionen komplexiert ist. Der Abstand zwischen parallelen DNA-Str{\"a}ngen h{\"a}ngt dabei von der Gr{\"o}ße der DNA-Fragmente sowie von der Art des Kations ab. Die gr{\"o}ßten Abst{\"a}nde ergeben sich mit Bariumionen, gefolgt von Magnesium- und Calciumionen. Die kleinsten DNA-Abst{\"a}nde werden durch Komplexierung mit Manganionen erhalten. Diese Ionenreihenfolge stellt sich sowohl f{\"u}r genomische DNA als auch f{\"u}r Plasmid-DNA ein. E) Die DNA-Abst{\"a}nde werden durch die Kompression des Lipidfilms nicht beeinflusst. Zwischen der Lipidmonoschicht und der adsorbierten DNA besteht demnach nur eine schwache Wechselwirkung. Offensichtlich befindet sich die durch zweiwertige Kationen komplexierte DNA als weitgehend eigenst{\"a}ndige Schicht unter dem Lipidfilm. 2. Modellsystem: Lipiddoppelschicht an der fest/fl{\"u}ssig-Grenzfl{\"a}che Methoden: Neutronenreflexion (NR) und Quarzmikrowaage (QCM-D) Resultate: A) Das zwitterionische Phospholipid DMPC (1,2-Dimyristoyl-phosphatidylcholin) bildet keine Lipiddoppelschicht auf planaren Polyelektrolytmultischichten aus, deren letzte Lage das positiv geladene PAH (Polyallylamin) ist. B) Hingegen bildet DMPC auf dem negativ geladenen PSS (Polystyrolsulfonat) eine Doppelschicht aus, die jedoch Defekte aufweist. C) Eine Adsorption von genomischer Kalbsthymus-DNA auf dieser Lipidschicht findet nur in Gegenwart von Calciumionen statt. Andere zweiwertige Kationen wurden nicht untersucht. D) Das negativ geladene Phospholipid DLPA (1,2-Dilauryl-phosphatids{\"a}ure) bildet auf dem positiv geladenen PAH eine Lipiddoppelschicht aus, die Defekte aufweist. E) DNA adsorbiert ebenfalls erst in Anwesenheit von Calciumionen in der L{\"o}sung an die DLPA-Schicht. F) Durch die Zugabe von EDTA (Ethylendiamintetraessigs{\"a}ure) werden die Calciumionen dem DLPA/DNA-Komplex entzogen, wodurch dieser dissoziiert. Demnach ist die calciuminduzierte Bildung dieser Komplexe reversibel.}, subject = {Lipide / Doppelschicht}, language = {de} } @article{Jeltsch2002, author = {Jeltsch, Florian}, title = {Wechselbeziehungen zwischen Artendiversit{\"a}t und struktureller Diversit{\"a}t : modellgest{\"u}tzte Untersuchungen am Beispiel einer semiariden Savanne}, year = {2002}, language = {de} } @article{WichmannJeltschDeanetal.2002, author = {Wichmann, Matthias and Jeltsch, Florian and Dean, Richard and Moloney, Kirk A. and Wissel, Christian}, title = {Weather does matter : simulating population dynamics of tawny eagle (Aquila rapax) under various rainfall scenarios}, year = {2002}, language = {en} }