@article{LieckfeldtSimonRosinZoelleretal.2004, author = {Lieckfeldt, Elke and Simon-Rosin, Ulrike and Z{\"o}ller, Daniela and Ebert, Berit and Kehr, Julia and Fisahn, Joachim}, title = {Spatio/temporal analysis of gene exression profiles within single cells and specific tissue types of developing Arabidopsis plants}, isbn = {3-00-011587-0}, year = {2004}, language = {en} } @article{ZimmermannRegiererKossmannetal.2004, author = {Zimmermann, P. and Regierer, Babette and Kossmann, Jens and Frossard, Emmanuel and Amrhein, Nikolaus and Bucher, Matthias}, title = {Differential expression of three purple acid phosphatases from potato}, issn = {1435-8603}, year = {2004}, abstract = {Three cDNAs encoding purple acid phosphatase (PAP) were cloned from potato (Solanum tuberosum L. cv. Desiree) and expression of the corresponding genes was characterised. StPAP1 encodes a low-molecular weight PAP clustering with mammalian, cyanobacterial, and other plant PAPs. It was highly expressed in stem and root and its expression did not change in response to phosphorus (P) deprivation. StIPAP2 and StPAP3 code for high-molecular weight PAPs typical for plants. Corresponding gene expression was shown to be responsive to the level of P supply, with transcripts of StPAP2 and StPAP3 being most abundant in P-deprived roots or both stem and roots, respectively. Root colonisation by arbuscular mycorrhizal fungi had no effect on the expression of any of the three PAP genes. StIPAP1 mRNA is easily detectable along the root axis, including root hairs, but is barely detectable in root tips. In contrast, both StPAP2 and StPAP3 transcripts are abundant along the root axis, but absent in root hairs, and are most abundant in the root tip. All three PAPs described contain a predicted N-terminal secretion signal and could play a role in extracellular P scavenging, P mobilisation from the rhizosphere, or cell wall regeneration}, language = {en} } @article{TiedemannPaulusScheeretal.2004, author = {Tiedemann, Ralph and Paulus, Kirsten B. and Scheer, M. and Von Kistowski, K. G. and Skirnisson, K. and Bloch, D. and Dam, M.}, title = {Mitochondrial DNA and microsatellite variation in the eider duck (Somateria mollissima) indicate stepwise postglacial colonization of Europe and limited current long-distance dispersal}, issn = {0962-1083}, year = {2004}, abstract = {To unravel the postglacial colonization history and the current intercolony dispersal in the common eider, Somateria mollissima, we analysed genetic variation at a part of the mitochondrial control region and five unlinked autosomal microsatellite loci in 175 eiders from 11 breeding colonies, covering the entire European distribution range of this species. As a result of extreme female philopatry, mitochondrial DNA differentiation is substantial both among local colonies and among distant geographical regions. Our study further corroborates the previous hypothesis of a single Pleistocene refugium for European eiders. A nested clade analysis on mitochondrial haplotypes suggests that (i) the Baltic Sea eider population is genetically closest to a presumably ancestral population and that (ii) the postglacial recolonization progressed in a stepwise fashion via the North Sea region and the Faroe Islands to Iceland. Current long-distance dispersal is limited. Differentiation among colonies is much less pronounced at microsatellite loci. The geographical pattern of this nuclear genetic variation is to a large extent explained by isolation by distance. As female dispersal is very limited, the geographical pattern of nuclear variation is probably explained by male-mediated gene flow among breeding colonies. Our study provides genetic evidence for the assumed prominent postglacial colonization route shaping the present terrestrial fauna of the North Atlantic islands Iceland and the Faroes. It suggests that this colonization had been a stepwise process originating in continental Europe. It is the first molecular study on eider duck populations covering their entire European distribution range}, language = {en} } @article{RavaoarimananaTiedemannMontagnonetal.2004, author = {Ravaoarimanana, I. B. and Tiedemann, Ralph and Montagnon, D. and Rumpler, Y.}, title = {Molecular and cytogenetic evidence for cryptic speciation within a rare endemic Malagasy lemur, the Northern Sportive Lemur (Lepilemur septentrionalis)}, issn = {1055-7903}, year = {2004}, abstract = {Evolutionary relationships of different populations of the threatened malagasy lemur Lepilemur septentrimialis were assessed by sequence analysis of mitochondrial DNA (D-loop region and partial Cyt b gene). One hundred and fifty nine samples were collected from five main different localities in the northern part of Madagascar. We applied the phylogenetic species concept based on fixed diagnostic differences to determine the status of different geographical populations. No nucleotide site diagnoses Ankarana from Andrafiamena or Analamera. However, numerous fixed differences separate Sahafary from all other populations. These results were corroborated by phylogenetic trees. As previous cytogenetic studies, our molecular data suggest that two cryptic species of Lepilemur occur in the extreme north of Madagascar. This speciation is probably caused by chromosomal rearrangements in at least one of the evolutionary lineages. Our study comprises another striking example of how molecular genetic assay can detect phylogenetic discontinuities that are not reflected in traditional morphologically based taxonomies. Our study indicates that the Sahafary population is a hitherto undescribed endangered endemic species which urgently needs conservation efforts. (C) 2003 Elsevier Inc. All rights reserved}, language = {en} } @article{MilinkovitchMonteyneGibbsetal.2004, author = {Milinkovitch, M. C. and Monteyne, D. and Gibbs, J. P. and Fritts, T. H. and Tapia, W. and Snell, H. L. and Tiedemann, Ralph and Caccone, Adalgisa and Powell, J. R.}, title = {Genetic analysis of a successful repatriation programme : giant Galapagos tortoises}, issn = {0962-8452}, year = {2004}, abstract = {As natural populations of endangered species dwindle to precarious levels, remaining members are sometimes brought into captivity, allowed to breed and their offspring returned to the natural habitat. One goal of such repatriation programmes is to retain as much of the genetic variation of the species as possible. A taxon of giant GalApagos tortoises on the island of Espahola has been the subject of a captive breeding-repatriation programme for 33 years. Core breeders, consisting of 12 females and three males, have produced more than 1200 offspring that have been released on Espanola where in situ reproduction has recently been observed. Using microsatellite DNA markers, we have determined the maternity and paternity of 132 repatriated offspring. Contributions of the breeders are highly skewed. This has led to a further loss of genetic variation that is detrimental to the long-term survival of the population. Modifications to the breeding programme could alleviate this problem}, language = {en} } @article{BrunkBeierBurkartetal.2004, author = {Brunk, Ingo and Beier, Wolfgang and Burkart, Bettina and Hinrichsen, Arne and Oehlschl{\"a}ger, Susanne and Prochnow, Annette and Saure, Christoph and Vorwald, J{\"o}rn and Wallschl{\"a}ger, Hans-Dieter and Zierke, Irene}, title = {Beweidung mit Haustieren}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{Poeste2004, author = {Poeste, Simon}, title = {Analyse von transgenen Kartoffelpflanzen mit ver{\"a}nderter cytosolischer Phosphorylase (Ph2) Aktivit{\"a}t}, pages = {102 S.}, year = {2004}, language = {de} } @article{WannerBurkartHinrichsenetal.2004, author = {Wanner, Manfred and Burkart, Bettina and Hinrichsen, Arne and Prochnow, Annette and Schlauderer, Ralf and Wiesener, Cornelia and Zierke, Irene and Xylander, Willi E. R.}, title = {Offenhaltung durch mechanische Bodenfreilegung}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{WannerAndersBrunketal.2004, author = {Wanner, Manfred and Anders, Kenneth and Brunk, Ingo and Burkart, Bettina and van Dorsten, Petra and F{\"u}rstenau, Stefan and Oehlschl{\"a}ger, Susanne and Prochnow, Annette and Wiesener, Cornelia and Xylander, Willi E. R.}, title = {Offenhaltung durch Feuer}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{AndersMrzljakWallschlaegeretal.2004, author = {Anders, Kenneth and Mrzljak, Jadranka and Wallschl{\"a}ger, Hans-Dieter and Wiegleb, Gerhard}, title = {Handbuch Offenlandmanagement am Beispiel ehemaliger und in Nutzung befindlicher Truppen{\"u}bungspl{\"a}tze}, publisher = {Springer}, address = {Berlin}, isbn = {3-540-22449-1}, pages = {320 S.}, year = {2004}, language = {de} } @article{HinrichsenAndersBeieretal.2004, author = {Hinrichsen, Arne and Anders, Kenneth and Beier, Wolfgang and van Dorsten, Petra and Oehlschl{\"a}ger, Susanne and Pilarski, Monika and Prochnow, Annette and Tsch{\"o}pe, Okka}, title = {Das Wildgehege Glauer Tal}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{BrunkAndersMaehnertetal.2004, author = {Brunk, Ingo and Anders, Kenneth and M{\"a}hnert, Pia and Mrzljak, Jadranka and Nocker, Ulrike and Saure, Christoph and Vorwald, J{\"o}rn and Borries, J{\"u}rgen and Wiegleb, Gerhard}, title = {Der ehemalige Truppen{\"u}bungsplatz Lieberose}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{MaehnertAndersBrunketal.2004, author = {M{\"a}hnert, Pia and Anders, Kenneth and Brunk, Ingo and Nocker, Ulrike and Pilarski, Monika and Mrzljak, Jadranka and Saure, Christoph and Vorwald, J{\"o}rn and Wiegleb, Gerhard}, title = {Der ehemalige Truppen{\"u}bungsplatz Bad Liebenwerda (Forsthaus Pr{\"o}sa)}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{BurkartGaertnerHarnischetal.2004, author = {Burkart, Bettina and Gaertner, Mirijam and Harnisch, Richard and Heyne, Peter and Konold, Werner and Schlauderer, Ralf and Segert, Astrid and Striese, Michael and Wanner, Manfred}, title = {Der ehemalige Panzerschießplatz Dauban}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{WannerAndersBischofetal.2004, author = {Wanner, Manfred and Anders, Kenneth and Bischof, Ronny and Brozio, Fritz and Burkart, Bettina and Prochnow, Annette and Riedel, Heidi and Schneider, Dieter and Wiesener, Cornelia and Zulka, Klaus Peter and Zumkowski-Xylander, Helga and Xylander, Willi E. R.}, title = {Aktiver Truppen{\"u}bungsplatz Oberlausitz}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{AndersBeierBrunketal.2004, author = {Anders, Kenneth and Beier, Wolfgang and Brunk, Ingo and Burkart, Bettina and Mrzljak, Anders and Oehlschl{\"a}ger, Susanne}, title = {Freie Sukzession und Offenlandmanagement}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{PilarskiNockerWagner2004, author = {Pilarski, Monika and Nocker, Ulrike and Wagner, Anja}, title = {Geografische Informationssysteme und Fernerkundung als Instrumente landschafts{\"o}kologischer Untersuchungen}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{SegertZierke2004, author = {Segert, Astrid and Zierke, Irene}, title = {Methodische Grundlagen der soziologischen Bewertung von Offenland}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{AndersProchnowSchlaudereretal.2004, author = {Anders, Kenneth and Prochnow, Annette and Schlauderer, Ralf and Wiegleb, Gerhard}, title = {Die Szenario-Methode als Instrument der Naturschutzplanung im Offenland}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{KonoldAndersBurkartetal.2004, author = {Konold, Werner and Anders, Kenneth and Burkart, Bettina and Schlauderer, Ralf and Segert, Astrid and Zierke, Irene}, title = {Naturschutz auf ehemaligen Truppen{\"u}bungspl{\"a}tzen: eine abschließende Betrachtung}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{ProchnowBrunkSegert2004, author = {Prochnow, Annette and Brunk, Ingo and Segert, Astrid}, title = {Offenhaltung durch M{\"a}hen und R{\"a}umen bzw. Mulchen}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{OehlschlaegerBeiervanDorstenetal.2004, author = {Oehlschl{\"a}ger, Susanne and Beier, Wolfgang and van Dorsten, Petra and Harnisch, Richard and Tsch{\"o}pe, Okka and Zierke, Irene}, title = {Das Naturschutzgebiet D{\"o}beritzer Heide}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{WarnckeVogtUlrichetal.2004, author = {Warncke, Max and Vogt, Birgit and Ulrich, Jacqueline and von Laer, Meike Dorothee and Beyer, Winfried and Klump, Hannes and Micheel, Burkhard and Sheriff, Ahmed}, title = {Efficient in vitro transduction of naive murine B cells with lentiviral vectors}, year = {2004}, abstract = {The aim of this study was to determine the impact of lentiviral transduction on primary murine B cells. Studying B cell activities in vivo or using them for tolerance induction requires that the cells remain unaltered in their biological behavior except for expression of the transgene. As we show here, murine B cells can efficiently be transduced by lentiviral, VSV-G-pseudotyped vectors without the necessity of prior activation. Culture with LPS gave enhanced transduction efficiencies but led to the upregulation of CD86 and proliferation of the cells. Transduction of naive B cells by lentiviral vectors was dependent on multiplicity of infection and did not lead to a concomitant activation. Furthermore, the transduced cells could be used for studies in the NOD mouse system without altering the onset of diabetes. We conclude that lentiviral gene transfer into naive B cells is a powerful tool for manipulation of B cells for therapeutic applications.}, language = {en} } @article{SchurrBossdorfMiltonetal.2004, author = {Schurr, Frank Martin and Bossdorf, Oliver and Milton, Sue J. and Schumacher, J.}, title = {Spatial pattern formation in semi-arid shrubland : a priori predicted versus observed pattern characteristics}, year = {2004}, abstract = {Ecologists increasingly use spatial statistics to study vegetation patterns. Mostly, however, these techniques are applied in a purely descriptive fashion without a priori statements on the pattern characteristics expected. We formulated such a priori predictions in a study of spatial pattern in a semi-arid Karoo shrubland, South Africa. Both seed dispersal and root competition have been discussed as processes shaping the spatial structure of this community. If either of the two processes dominates pattern formation, patterns within and between shrub functional groups are expected to show distinct deviations from null models. We predicted the type and scale of these deviations and compared predicted to observed pattern characteristics. As predicted by the seed dispersal hypothesis, small-scale co-occurrence within and between groups of colonisers and successors was increased as compared to complete spatially random arrangement of shrubs. The root competition predictions, however, were not met as shrubs of similar rooting depth co- occurred more frequently than expected under random shrub arrangement. Since the distribution of rooting groups to the given shrub locations also failed to match the root competition predictions, there was little evidence for dominance of root competition in pattern formation. Although other processes may contribute to small-scale plant co-occurrence, the sufficient and most parsimonious explanation for the observed pattern is that its formation was dominated by seed dispersal. To characterise point patterns we applied both cumulative (uni- and bivariate K-function) and local (pair- and mark-correlation function) techniques. Based on our results we recommend that future studies of vegetation patterns include local characteristics as they independently describe a pattern at different scales and can be easily related to processes changing with interplant distance in a predictable fashion.}, language = {en} } @article{SchurrDeanMiltonetal.2004, author = {Schurr, Frank Martin and Dean, W. R. J. and Milton, Sue J. and Jeltsch, Florian}, title = {A conceptual model linking demography of the shrub species Grewia flava to the dynamics of Kalahari savannas}, year = {2004}, abstract = {Environmental heterogeneity is a major determinant of plant population dynamics. In semi-arid Kalahari savannas, heterogeneity is created by savanna structure, i.e. by the spatial arrangement and temporal dynamics of woody plant and open grassland microsites. We formulate a conceptual model describing the effects of savanna dynamics on the population dynamics of the animal-dispersed shrub Grewia flava. From empirical results we derive model rules describing effects of savanna structure on several processes in Grewia's life cycle. By formulating the model, we summarise existing information on Grewia demography and identify gaps in this knowledge. Despite a number of such gaps, the model can be used to make certain quantitative predictions. As an example, we apply the model to investigate the role of seed dispersal in Grewia encroachment on rangelands. Model results show that cattle promote encroachment by depositing substantial numbers of seeds in open areas, where Grewia is otherwise dispersal-limited. Finally, we draw some general conclusions about Grewia's life history and population dynamics. Under natural conditions, concentrated seed deposition under woody plants appears to be a key process causing the observed association between Grewia and other woody plants. Furthermore, low rates of recruitment and high adult survival result in slow-motion dynamics of Grewia populations. As a consequence, Grewia populations interact with savanna dynamics on long temporal and short to intermediate spatial scales.}, language = {en} } @article{Baumann2004, author = {Baumann, Otto}, title = {Konventionelle Fluoreszenzmikroskopie : Theorie und Anwendungsm{\"o}glichkeiten}, year = {2004}, language = {de} } @article{BeissenhirtzSchellerStoeckleinetal.2004, author = {Beissenhirtz, Moritz Karl and Scheller, Frieder W. and St{\"o}cklein, Walter F. M. and Kurth, D. and M{\"o}hwald, Helmuth and Lisdat, Fred}, title = {Electroactive cytochrome c multilayers within a polyelectrolyte assembly}, year = {2004}, language = {en} } @article{LettauGajovicEichelmannKwaketal.2004, author = {Lettau, Kristian and Gajovic-Eichelmann, N. and Kwak, Young-Keun and Scheller, Frieder W. and Warsinke, Axel}, title = {Hydroxylasen und katalytische Polymere f{\"u}r Biochips}, year = {2004}, language = {de} } @article{KroeningSchellerWollenbergeretal.2004, author = {Kr{\"o}ning, Steffen and Scheller, Frieder W. and Wollenberger, Ursula and Lisdat, Fred}, title = {Myoglobin-Clay Electrode for Nitric Oxide (NO) Detection in Solution}, year = {2004}, language = {en} } @article{SchellerTiepnerWarsinke2004, author = {Scheller, Frieder W. and Tiepner, K. and Warsinke, Axel}, title = {Anwendung von Biosensoren in der Lebensmittelanalytik}, isbn = {3-89947-120-2}, year = {2004}, language = {de} } @article{KernKnoesche2004, author = {Kern, J{\"u}rgen and Kn{\"o}sche, R{\"u}diger}, title = {Landwirtschaft und Gew{\"a}sserschutz im Land Brandenburg}, isbn = {3-89998-033-6}, year = {2004}, language = {de} } @article{Kummer2004, author = {Kummer, Volker}, title = {Vom Seidenbau in Krausnick}, year = {2004}, language = {de} } @article{Kummer2004, author = {Kummer, Volker}, title = {Dr. Dieter Benkert zum 70. Geburtstag}, issn = {0724-3111 -}, year = {2004}, language = {de} } @article{Kummer2004, author = {Kummer, Volker}, title = {Bemerkenswerte Pilzfunde auf der 34. Brandenburgischen Botanikertagung in Ortrand}, issn = {0724-3111 -}, year = {2004}, language = {de} } @article{Kummer2004, author = {Kummer, Volker}, title = {Beitr{\"a}ge zur Pilzflora der Luckauer Umgebung : die Makromyceten der Pilzexkursion am 05.10.02 bei Prierow}, year = {2004}, language = {de} } @article{Kummer2004, author = {Kummer, Volker}, title = {Beitr{\"a}ge zur Pilzflora des Spreewaldes : II. Die Myxo- und Makromyceten (Ascomycetes, Basidiomycetes) im Bereich des Neuendorfer Sees (Biosph{\"a}renreservat Spreewald) ; Teil 2}, year = {2004}, language = {de} } @article{AleAghaBolayBraunetal.2004, author = {Ale-Agha, Nosratollah and Bolay, Adrien and Braun, Uwe and Jage, Horst and Kummer, Volker and Lebeda, Ales and Piatek, Marcin and Shin, Hyeon-Dong and Zimmermannova-Pastircakova, Katarina}, title = {Erysiphe catalpae and E. elevata in Europe}, year = {2004}, language = {en} } @article{JeltschWichmannDean2004, author = {Jeltsch, Florian and Wichmann, Matthias and Dean, W. R. J.}, title = {Global change challenges the Tawny Eagle (Aquila rapax) : modelling extinction risk with respect to predicted climate and land use changes}, year = {2004}, language = {en} } @article{Illner2004, author = {Illner, Regine}, title = {Gruppenpuzzle im Hochschulseminar : selbst erfahren, wie kooperatives lernen funktioniert}, series = {Journal f{\"u}r LehrerInnenbildung. - 4 (2004), 3, S. 67 - 71}, journal = {Journal f{\"u}r LehrerInnenbildung. - 4 (2004), 3, S. 67 - 71}, editor = {Altrichter, Herbert and Horstkemper, Marianne}, year = {2004}, language = {de} } @article{RohdeHinchaHeyer2004, author = {Rohde, P. and Hincha, Dirk K. and Heyer, Arnd G.}, title = {Heterosis in the freezing tolerance of crosses between two Arabidopsis thaliana accessions (Columbia-0 and C24) that show differences in non-acclimated and acclimated freezing tolerance}, issn = {0960-7412}, year = {2004}, language = {en} } @article{VerginFreierGzik2004, author = {Vergin, Anika and Freier, B. and Gzik, Axel}, title = {Investigations on effects of plant strengthening products to tri-trophic system field bean (Vicia faba (L.)) - black bean aphid (Aphis fabae Scop.) - lacewing larva (Chrysoperla carnea Steph.)}, year = {2004}, language = {en} } @article{SchellerWagener2004, author = {Scheller, Frieder W. and Wagener, C.}, title = {From gene to life}, year = {2004}, language = {en} } @article{SauerWartenbergSachinidisetal.2004, author = {Sauer, Heinrich and Wartenberg, Maria and Sachinidis, A. and Hescheler, J{\"u}rgen}, title = {The development of the cardiovascular system in embryoid bodies deriverd from embryonic stem cells}, isbn = {1-588- 29113-8}, year = {2004}, language = {en} } @article{RhoadesCohenGussakovskyetal.2004, author = {Rhoades, E. and Cohen, M. and Gussakovsky, E. and Schuler, Benjamin and Haran, G.}, title = {Single molecule protein folding}, issn = {0006-3495}, year = {2004}, language = {en} } @article{ObelUsadelChooetal.2004, author = {Obel, Nicolai and Usadel, Bj{\"o}rn and Choo, Tze Siang and Pauly, Markus}, title = {Analysing cell wall biosynthesis to study its role in biotic and abiotic stress reactions}, isbn = {3-00-011587-0}, year = {2004}, language = {en} } @article{CleggMaberlyJones2004, author = {Clegg, Mark R. and Maberly, Stephen C. and Jones, Roger I.}, title = {Dominance and compromise in freshwater phytoplanktonic flagellates : the interaction of behavioural preferences for conflicting environmental gradients}, year = {2004}, language = {en} } @article{TittelKamjunke2004, author = {Tittel, J{\"o}rg and Kamjunke, Norbert}, title = {Metabolism of dissolved organic carbon by planktonic bacteria and mixotrophic algae in lake neutralisation experiments}, year = {2004}, abstract = {1. Lakes formed in mining pits often contain high concentrations of dissolved ferric iron and sulphate (e.g. 2 and 16 mmol L)1, respectively) and the pH is buffered between 2.5 and 3.5. Efforts to neutralise their water are based on the stimulation of lake internal, bacterial iron- and sulphate reduction. Electron donors may be supplied by organic carbon compounds or indirectly by enhancement of primary production. Here, we investigated the function of mixotrophic algae, which can potentially supplement or deplete the organic carbon pool, in the carbon metabolism and alkalinity budget of an acidic mining lake. 2. Two weeks after organic substrates had been added in a large in situ mesocosm of 30 m diameter, a bloom of Chlamydomonas occurred, reaching a biovolume of 80 mm3 L)1. Growth experiments using filtered lake water showed that the alga reduced the overall dissolved organic carbon (DOC) concentration despite significant photosynthetic activity. However, when Chlamydomonas were grown together with natural bacterioplankton, net DOC consumption did not increase. 3. Uptake experiments using [14C]-glucose indicated that bacteria dominated glucose uptake and remineralisation. Therefore, the DOC leached in the water column was processed mainly by planktonic bacteria. Leached DOC must be regarded as loss, not transferred by larger organisms to the sediment, where reduction processes take place. 4. From phytoplankton biomass and production 2 years after fertilisation we estimated that pelagic photosynthesis does not supply an electron donor capacity capable of reducing more than 2\% of actual stock of acidity per year. We estimated that only the benthic primary production was in a range to compensate for ongoing inputs of iron and sulphate.}, language = {en} } @article{Weithoff2004, author = {Weithoff, Guntram}, title = {Vertical niche separation of two consumers (Rotatoria) in an extreme habitat}, year = {2004}, abstract = {Herbivore populations are commonly restricted by resource limitation, by predation or a combination of the two. Food supplement experiments are suitable for investigating the extent of food limitation at any given time. The main part of this study was performed in an extremely acidic lake (pH 2.7) where the food web consists of only a few components and potential food sources for herbivores are restricted to two flagellates. Life table experiments proved that Chlamydomonas was a suitable food source whereas Ochromonas was an unsuitable food source. The two flagellates and the two rotifers exhibit a pronounced vertical distribution pattern. In this study, a series of food supplement experiments were performed in order to: (1) quantify and compare potential resource limitation of two primary consumers (Cephalodella hoodi and Elosa worallii, Rotatoria) over time, (2) compare their response at different temperatures, (3) evaluate the effect of having an unsuitable food source alongside a valuable one, (4) estimate the effect of predation on rotifers by Heliozoa, and (5) compare the results with those from other acidic lakes. Additionally, the spatio- temporal population dynamics of both species were observed. The field data confirmed a vertical separation of the two species with E. worallii dominating in the upper water layers, and C. hoodi in the deeper, cooler water layers. The results from the food supplement experiments in which Chlamydomonas served as the supplemented suitable food source showed that the two rotifers were food limited in the epilimnion throughout the season to different extents, with Cephalodella being more severely food limited than Elosa. The experiments at different temperatures provided evidence that Elosa had a higher optimum temperature for growth than Cephalodella. When the unsuitable food algae Ochromonas was added alongside the suitable food source Chlamydomonas, C. hoodi was unaffected but E. worallii was negatively affected. Predation of Heliozoa on rotifers was observed but the total effect on the rotifer dynamics is probably low. The comparison with other lakes showed that resource limitation also occurred in one other lake, although to a lesser extent. Overall, the vertical separation of the two rotifers could be explained by both their differential extent of resource limitation and differential response to temperature.}, language = {en} } @article{KoechyWilson2004, author = {K{\"o}chy, Martin and Wilson, Scott D.}, title = {Semiarid grassland responses to short-term variation in water availability}, issn = {1385-0237}, year = {2004}, abstract = {Standing crop and species composition in semiarid grassland are linked to long-term patterns of water availability, but grasslands are characterized by large single-season variability in rainfall. We tested whether a single season of altered water availability influenced the proportions of grasses and shrubs in a semiarid grassland near the northern edge of the North American Great Plains. We studied stands of the clonal shrub snowberry (Symphoricarpos occidentalis) and adjacent grassland dominated by the native grasses Stipa spartea and Bouteloua gracilis. Rain was excluded and water supplied in amounts corresponding to years of low, medium, and high rainfall, producing a 2 - 4-fold range in monthly precipitation among water supply treatments. There were ten replicate plots of each water treatment in both snowberry stands and grassland. Grass standing crop increased significantly with water availability in grassland but not inside snowberry stands. Total standing crop and shrub stem density increased significantly with water supply, averaged across both communities. In contrast, water had no effect on shrub standing crop or light penetration. In summary, our finding that water has significant effects on a subset of components of grassland vegetation is consistent with long-term, correlational studies, but we also found that a single season of altered water supply had no effect on other important aspects of the ecosystem.}, language = {en} } @article{FischerPfistererJoshietal.2004, author = {Fischer, Markus and Pfisterer, A. and Joshi, Jasmin Radha and Schmid, Bernhard}, title = {Rapid decay of diversity-productivity relationships after invasion of experimental plant communities}, year = {2004}, language = {en} } @article{HanischvanRossumGastetal.2004, author = {Hanisch, Uwe-Karsten and van Rossum, D. and Gast, Klaus and Misselwitz, Rolf and Goldstein, Gundars and Koistinaho, Jari and M{\"o}ller, Thomas}, title = {The microglia-activating potential of thrombin : is the protease able to induce cyto- and chemokines?}, year = {2004}, language = {en} } @article{SchefflerKetelhutMorgenstern2004, author = {Scheffler, Christiane and Ketelhut, Kerstin and Morgenstern, Ulrike}, title = {K{\"o}rperliche und motorische Entwicklung von Kindern unterschiedlicher sozialer Herkunft.}, year = {2004}, language = {de} } @article{BergerToerjekAltmann2004, author = {Berger, Dieter and T{\"o}rjek, Otto and Altmann, Thomas}, title = {Establishment of high-efficiency mapping tools}, isbn = {3-00-011587-0}, year = {2004}, language = {en} } @article{JandrigSeitzHinzmannetal.2004, author = {Jandrig, Burkhard and Seitz, Susanne and Hinzmann, Bernd and Arnold, Wolfgang and Micheel, Burkhard and Koelble, Konrad and Siebert, Reiner and Schwartz, Arnfried and Ruecker, Karin and Schlag, Peter M. and Scherneck, Siegfried and Rosenthal, Andra}, title = {ST18 is a breast cancer tumor suppressor gene at human chromosome 8q11.2}, year = {2004}, abstract = {We have identified a gene, ST18 (suppression of tumorigenicity 18, breast carcinoma, zinc-finger protein), within a frequent imbalanced region of chromosome 8q11 as a breast cancer tumor suppressor gene. The ST18 gene encodes a zinc-finger DNA-binding protein with six fingers of the C2HC type (configuration Cys-X5-Cys-X12-His-X4-Cys) and an SMC domain. ST18 has the potential to act as transcriptional regulator. ST18 is expressed in a number of normal tissues including mammary epithelial cells although the level of expression is quite low. In breast cancer cell lines and the majority of primary breast tumors, ST18 mRNA is significantly downregulated. A 160 bp region within the promoter of the ST18 gene is hypermethylated in about 80\% of the breast cancer samples and in the majority of breast cancer cell lines. The strong correlation between ST18 promoter hypermethylation and loss of ST18 expression in tumor cells suggests that this epigenetic mechanism is responsible for tumor-specific downregulation. We further show that ectopic ST18 expression in MCF-7 breast cancer cells strongly inhibits colony formation in soft agar and the formation of tumors in a xenograft mouse model}, language = {en} } @article{PiontekWinklerBaletal.2004, author = {Piontek, J. and Winkler, Lars and Bal, M. S. and Lassowski, Birgit and Mueller, Sandra L. and Gast, Klaus and Blasig, Ingolf E.}, title = {Investigating of homophilic interactions of the tight junction proteins occludin and claudin-5}, year = {2004}, language = {en} } @article{LehnhardtPufeBaumanetal.2004, author = {Lehnhardt, Lothar and Pufe, Heidrun and Bauman, Ingrid and Baumann, Guido}, title = {Responses of chloroplast thylakoid composition to low temperature and high light stress in callus cultures and in leaves of sugar beet}, year = {2004}, language = {en} } @article{KleuserStoeckleinPieperFuerstetal.2004, author = {Kleuser, U. and St{\"o}cklein, Walter F. M. and Pieper-F{\"u}rst, U. and Scheller, Frieder W.}, title = {Partikelverst{\"a}rkte Oberfl{\"a}chenplasmonresonanz f{\"u}r die Quantifizierung von Matrix Metalloproteinase-2}, year = {2004}, language = {de} } @article{WollenbergerBistolasJungetal.2004, author = {Wollenberger, Ursula and Bistolas, Nikitas and Jung, Christiane and Shumyantseva, V. V. and Ruzgas, T. and Scheller, Frieder W.}, title = {Elektroden-Design f{\"u}r elektronische Wechselwirkung mit Monooxygenasen}, isbn = {3-8047-2132-x}, year = {2004}, language = {de} } @article{JeltschWiegandWard2004, author = {Jeltsch, Florian and Wiegand, K. and Ward, D.}, title = {Seed dispersal by cattle may cause shrub encroachment of Grewia flava on southern Kalahari rangelands}, year = {2004}, language = {en} } @article{ScheibleToerjekAltmann2004, author = {Scheible, W. R. and T{\"o}rj{\´e}k, Otto and Altmann, Thomas}, title = {From markers to cloned genes : map based cloning}, isbn = {3-540-20689-2}, year = {2004}, language = {en} } @article{SachinidisWartenbergSaueretal.2004, author = {Sachinidis, A. and Wartenberg, Maria and Sauer, Heinrich and Hescheler, J{\"u}rgen}, title = {Transcription factors, growth factors and signal cascades capable of priming morphogenesis of heart}, isbn = {1-588- 29113-8}, year = {2004}, language = {en} } @article{TschoepeBeierBurkartetal.2004, author = {Tsch{\"o}pe, Okka and Beier, Wolfgang and Burkart, Bettina and Hinrichsen, Arne and Katscher, Kathleen and Oehlschl{\"a}ger, Susanne and Prochnow, Annette and Schlauderer, Ralf and Segert, Astrid and Siniza, Swetlana and van Dorsten, Petra}, title = {Beweidung mit Wildtieren}, isbn = {3-540-22449-1}, year = {2004}, language = {de} } @article{KamjunkeGaedkeTitteletal.2004, author = {Kamjunke, Norbert and Gaedke, Ursula and Tittel, J{\"o}rg and Weithoff, Guntram and Bell, Elanor M.}, title = {Strong vertical differences in the plankton composition of an extremely acidic lake}, year = {2004}, abstract = {Vertical differences in food web structure were examined in an extremely acidic, iron-rich mining lake in Germany (Lake 111; pH 2.6, total Fe 150mg L-1) during the period of stratification. We tested whether or not the seasonal variation of the plankton composition is less pronounced than the differences observed over depth. The lake was strongly stratified in summer, and concentrations of dissolved organic carbon and inorganic carbon were consistently low in the epilimnion but high in the hypolimnion. Oxygen concentrations declined in the hypolimnion but were always above 2mg L-1. Light attenuation did not change over depth and time and was governed by dissolved ferric iron. The plankton consisted mainly of single-celled and filamentous bacteria, the two mixotrophic flagellates Chlamydomonas sp. and Ochromonas sp., the two rotifer species Elosa worallii and Cephalodella hoodi, and Heliozoa as top predators. We observed very few ciliates and rhizopods, and no heterotrophic flagellates, crustaceans or fish. Ochromonas sp., bacterial filaments, Elosa and Heliozoa dominated in the epilimnion whereas Chlamydomonas sp., single-celled bacteria and Cephalodella dominated in the hypolimnion. Single-celled bacteria were controlled by Ochromonas sp. whereas the lack of large consumers favoured a high proportion of bacterial filaments. The primarily phototrophic Chlamydomas sp. was limited by light and CO2 and may have been reduced due to grazing by Ochromonas sp. in the epilimnion. The distribution of the primarily phagotrophic Ochromonas sp. and of the animals seemed to be controlled by prey availability. Differences in the plankton composition were much higher between the epilimnion and hypolimnion than within a particular stratum over time. The food web in Lake 111 was extremely species-poor enabling no functional redundancy. This was attributed to the direct exclusion of species by the harsh environmental conditions and presumably enforced by competitive exclusion. The latter was promoted by the low diversity at the first trophic level which, in turn, was attributed to relatively stable growth conditions and the independence of resource availability (inorganic carbon and light) from algal density. Ecological theory suggests that low functional redundancy promotes low stability in ecosystem processes which was not supported by our data.}, language = {en} } @article{LagusSuomelaWeithoffetal.2004, author = {Lagus, A. and Suomela, J. and Weithoff, Guntram and Heikkila, K. and Helminen, H. and Sipura, J.}, title = {Species-specific differences in phytoplankton responses to N and P enrichments and the N:P ratio in the Archipelago Sea, northern Baltic Sea}, year = {2004}, abstract = {A nutrient enrichment experiment was conducted in order to study the role of nitrogen (N), phosphorus (P) and the N:P ratio on the early summer phytoplankton community in the Archipelago Sea, northern Baltic Sea. The phytoplankton community was, in terms of chlorophyll a and total biomass, primarily N-limited, but the individual species varied in their responses to the nutrient supply. The recorded overall N limitation was due to fast growth responses of a few N- limited species such as the diatom Chaetoceros wighamii (Brightwell) and the mixotrophic chrysophyte Uroglena sp. Another dominating diatom, Skeletonema costatum (Greville) Cleve was most clearly P-limited. The N:P ratio had the strongest effect on Uroglena sp., which grew exponentially in the enrichments with a high N:P ratio. This can be explained by the ability of the species to feed on P-rich bacteria, which gives it a competitive advantage in P-limited conditions. The species-specific differences in the responses to the nutrient enrichments can generally be explained by differences in the species physiology and they were consistent with the theory of resource competition.}, language = {en} } @article{KetelhutMohassebScheffleretal.2004, author = {Ketelhut, Kerstin and Mohasseb, Iman and Scheffler, Christiane and Ketelhut, Reinhard G.}, title = {Regular exercise improves risk profile and motor development in early childhood}, year = {2004}, language = {en} } @article{MeyerMuessigAltmann2004, author = {Meyer, Rhonda C. and M{\"u}ssig, Carsten and Altmann, Thomas}, title = {Genetic Diversity : Creation of novel genetic variants of arabidopsis}, isbn = {3-00-011587-0}, year = {2004}, language = {en} } @article{RahimiIsachenkoSaueretal.2004, author = {Rahimi, G. and Isachenko, E. and Sauer, Heinrich and Isachenko, V. and Wartenberg, Maria and Tawadros, S. and Hescheler, J{\"u}rgen and Mallmann, P. and Nawroth, F.}, title = {Comparison of necrosis in human ovarian tissue after conventional slow freezing or vitrification and transplantation in ovarectomized SCID mice}, year = {2004}, language = {en} } @article{FischerStoecklinWeyandetal.2004, author = {Fischer, Markus and St{\"o}cklin, J. and Weyand, Anne and Maurer, Katrin}, title = {Cultural and biological diversity of grasslands in the Swiss Alps}, isbn = {3-7281-2940-2}, year = {2004}, language = {en} } @article{GrosseBrauckmannKummer2004, author = {Grosse-Brauckmann, Helga and Kummer, Volker}, title = {F{\"u}nf bemerkenswerte Funde corticioider Pilze aus Deutschland}, issn = {0014-8962}, year = {2004}, language = {de} } @article{FischerLienert2004, author = {Fischer, Markus and Lienert, J.}, title = {Experimental inbreeding reduces seed production and germination independent of fragmentation of populations of Swertia perennis}, year = {2004}, language = {en} } @article{SchellerWarsinkePfeifferetal.2004, author = {Scheller, Frieder W. and Warsinke, Axel and Pfeiffer, Dorothea and Czeponik, J.}, title = {Biosensorik / Bioanalytik}, isbn = {3-87081-372-5}, year = {2004}, language = {de} } @article{JeltschTews2004, author = {Jeltsch, Florian and Tews, J{\"o}rg}, title = {Climate change impacts woody plant population dynamics in arid savanna}, year = {2004}, language = {en} } @article{HeschelerWartenbergWenzeletal.2004, author = {Hescheler, J{\"u}rgen and Wartenberg, Maria and Wenzel, D. and Roell, B. K. and Lu, Z. and Xia, Y. and Donmez, F. and Ling, Frederike C. and Acker, Helmut and Kolossov, E. and Kazemi, S. and Sasse, P. and Raible, A. and Bohlen, H. and Fleischmann, B. K.}, title = {Implication of therapeutic cloning for organ transplantation}, year = {2004}, language = {en} } @article{SauerNeukirchenRahimietal.2004, author = {Sauer, Heinrich and Neukirchen, W. and Rahimi, G. and Gr{\"u}nbeck, Frank and Hescheler, J{\"u}rgen and Wartenberg, Maria}, title = {Involvement of reactive oxygen species in cardiotropin-1 (CT-1)-induced cell proliferation of cardiomyocytes differentiated from murine embryonic stem cells}, year = {2004}, language = {en} } @article{KleinFeldhahnHarderetal.2004, author = {Klein, Florian and Feldhahn, Niklas and Harder, S. and Wang, Hui and Wartenberg, Maria and Hofmann, W.-K. and Wernet, Peter and Sieber, Reiner and M{\"u}schen, Markus}, title = {The BCR-ABL1 kinase bypasses selection for the expressio of a pre-B cell receptor in pre-B acute lymphoblastic leukemia cells}, year = {2004}, language = {en} } @article{AckerHuckstorfSaueretal.2004, author = {Acker, Helmut and Huckstorf, Christine and Sauer, Heinrich and Streller, Tino and Wartenberg, Maria}, title = {Deciphering the oxygen sensing pathway by microscopy}, year = {2004}, language = {en} } @article{BoeseGraySimmons2004, author = {Boese, Stefan H. and Gray, Michael A. and Simmons, N. L.}, title = {Volume and non-volume activated anion conductances and their interactions in the renal IMCD}, isbn = {0-387- 23299-0}, year = {2004}, language = {en} } @article{BeathamMiddletonRomeroetal.2004, author = {Beatham, Jane L. and Middleton, A. and Romero, Rosario and VanderVen, Peter F. M. and Blanco, Gonzalo}, title = {Functional characterisation of the Ky protein}, issn = {0960-8966}, year = {2004}, language = {en} } @article{TrippoKoebnickZunftetal.2004, author = {Trippo, Ulrike and Koebnick, Corinna and Zunft, Hans-Joachim Franz and Greil, Hans}, title = {Bioelectrical impedance analysis for predicting body composition : what about the external validity of new regression equations?}, issn = {0002-9165}, year = {2004}, language = {en} } @article{KrylovBeissenhirtzAdamzigetal.2004, author = {Krylov, Andrey V. and Beissenhirtz, Moritz Karl and Adamzig, Holger and Scheller, Frieder W. and Lisdat, Fred}, title = {Thick-film electrodes for measurement of superoxide and hydrogen peroxide based on direct protein-electrode contacts}, year = {2004}, abstract = {Cytochrome c was immobilized on screen-printed thick-film gold electrodes by a self-assembly approach using mixed monolayers of mercaptoundecanoic acid and mercaptoundecanol. Cyclic voltammetry revealed quasi-reversible electrochemical behavior of the covalently fixed protein with a formal potential of +10 mV vs. Ag/AgCl. Polarized at +150 mV vs. Ag/AgCl the electrode was found to be sensitive to superoxide radicals in the range 300-1200 nmol L-1. Compared with metal needle electrodes sensitivity and reproducibility could be improved and combined with the easiness of preparation. This allows the fabrication of disposable sensors for nanomolar superoxide concentrations. By changing the electrode potential the sensor can be switched from response to superoxide radicals to hydrogen peroxide-another reactive oxygen species. H2O2 sensitivity can be provided in the range 10-1000 mumol L-1 which makes the electrode suitable for oxidative stress studies}, language = {en} } @article{SchefflerKetelhutMohassebetal.2004, author = {Scheffler, Christiane and Ketelhut, Kerstin and Mohasseb, Iman and Ketelhut, Reinhard G.}, title = {The influence of an exercise program on body composition, motor and cardiovascular parameters in pre-school children : a longitudinal study}, isbn = {88-87814-25-2}, year = {2004}, language = {en} } @article{DiezTauerSchulz2004, author = {Diez, Isabel and Tauer, Klaus and Schulz, Burkhard}, title = {Polypyrrole tubes via casting of pyrrole-beta-naphthalenesulfonic acid rods}, issn = {0303-402X}, year = {2004}, abstract = {A comprehensive study gives experimental evidence that a complex made from pyrrole and beta-naphthalenesulfonic acid in a molar composition of 3:1 acts as morphological precursor in the subsequent oxidative polymerization of pyrrole initiated with ammonium peroxodisulfate. The precursor complex itself is unable to polymerize but its outer parallelepipedal shape with a high aspect ratio is templated in the inner surface of the formed conducting polypyrrole tubes}, language = {en} } @article{GehmlichGeierOsterzieletal.2004, author = {Gehmlich, Katja and Geier, C. and Osterziel, Karl Joseph and F{\"u}rst, Dieter Oswald}, title = {Mutant muscle LIM protein is associated with hypertrophic cardiomyopathy and exhibits altered binding properties in the system MLP - N-RAP - alpha-actinin}, issn = {0171-9335}, year = {2004}, language = {en} } @article{MohassebKetelhutMohassebetal.2004, author = {Mohasseb, Iman and Ketelhut, Kerstin and Mohasseb, Iman and Scheffler, Christiane and Gericke, Christian and Scholze, J. and Ketelhut, Reinhard G.}, title = {Benefit of regular Exercise on Cardiovascular Risk Factors and Motor Development in Early Childhood}, year = {2004}, language = {en} } @article{FrischSanter2004, author = {Frisch, D. and Santer, B.}, title = {Temperature-induced responses of a permanent-pond and a temporary-pond cyclopoid copepod : a link to habitat predictability?}, issn = {1522-0613}, year = {2004}, abstract = {Temporary-pond species can be expected to use environmental cues to predict the onset of adverse conditions, while permanent-pond species may be insensitive to such cues. Temperature is such a potential cue in temporary waterbodies, as if fluctuates more widely with decreasing pond size than in deeper permanent ponds. We compared the temperature-induced response of a permanent-pond and a temporary-pond cyclopoid copepod focusing on juvenile development duration, diapause induction and survival during diapause. Nonlinear regression analysis suggested a stronger effect of temperature on the duration of juvenile development in the temporary-pond species. This species also showed a higher and temperature-dependent variation in development duration (highest coefficient of variation 26\%) compared with the permanent species, for which variation was lower and similar at all temperatures (maximal coefficient of variation 6\%). Temperature significantly influenced the induction of diapause in the temporary-pond species, where the percentage of individuals entering diapause increased from 0\% at 5degreesC and 10degreesC to 63\% at 15degreesC and 91\% at 20degreesC. In the permanent-pond species, diapause induction was independent of temperature and was induced in 100\% of experimental specimens. This suggests an obligatory diapause in the permanent-pond species, a type of dormancy that has not been described previously for cyclopoid copepods. Survival during diapause in both species was higher when the diapausing copepodid stage was reached at lower temperatures. At higher temperatures, the temporary-pond species survived longer than the permanent-pond species. These results suggest different temperature optima of the two species. The strategy displayed by the permanent-pond species might be selected for in more stable habitats and may preclude the colonization of temporary ponds. Higher flexibility in life-history traits and the use of temperature as an environmental cue in the temporary-pond species could be favoured in unpredictable habitats}, language = {en} } @article{GehmlichGeierOsterzieletal.2004, author = {Gehmlich, Katja and Geier, C. and Osterziel, Karl Joseph and VanderVen, Peter F. M. and F{\"u}rst, Dieter Oswald}, title = {Decreased interactions of mutant muscle LIM protein (MLP) with N-RAP and alpha-actinin and their implication for hypertrophic cardiomyopathy}, issn = {0302-766X}, year = {2004}, abstract = {Previous work has shown that mutations in muscle LIM protein (MLP) can cause hypertrophic cardiomyopathy (HCM). In order to gain an insight into the molecular basis of the disease phenotype, we analysed the binding characteristics of wild-type MLP and of the (C58G) mutant MLP that causes hypertrophic cardiomyopathy. We show that MLP can form a ternary complex with two of its previously documented myofibrillar ligand proteins, N-RAP and alpha-actinin, which indicates the presence of distinct, non-overlapping binding sites. Our data also show that, in comparison to wild-type MLP, the capacity of the mutated MLP protein to bind both N-RAP and alpha-actinin is significantly decreased. In addition, this single point mutation prevents zinc coordination and proper folding of the second zinc-finger in the first LIM domain, which consequently renders the protein less stable and more susceptible to proteolysis. The molecular basis for HCM-causing mutations in the MLP gene might therefore be an alteration in the equilibrium of interactions of the ternary complex MLP-N-RAP-alpha-actinin. This assumption is supported by the previous observation that in the pathological situation accompanied by MLP down regulation, cardiomyocytes try to compensate for the decreased stability of MLP protein by increasing the expression of its ligand N-RAP, which might finally result in the development of myocyte disarray that is characteristic of this disease}, language = {en} } @article{GibbsReevesSunnaetal.2004, author = {Gibbs, Moreland D. and Reeves, Rosalind A. and Sunna, Anwar and Bergquist, Peter L.}, title = {A yeast intron as a translational terminator in a plasmid shuttle vector}, issn = {1567-1356}, year = {2004}, abstract = {Plasmid shuttle vectors that contain both prokaryotic (Escherichia coli) and eukaryotic origins of replication are routinely used in molecular biology since E coli is generally the organism of choice for manipulation of recombinant DNA. Initial transformation of the shuttle vector into E coli allows production of microgram quantities of DNA suitable for transformation of low-transformationefficiency hosts. A shuttle/expression vector for the yeast Kluyveromyces lactis, pCWK1, allows recombinant protein fused to the killer toxin signal sequence to be secreted to the medium. The heterologous genes are transcribed under the control of the K lactis LAC4 promoter, which is tightly regulated in K lactis. However, in E coli the LAC4 promoter functions constitutively, and as a result, uncontrolled transcription and translation of genes that are toxic in E coli can result in cell death, and subsequent failure to recover intact E. coli transformants. We have constructed and tested a modified shuttle vector that contains a K lactis ribosomal intron that acts as a translational terminator in E coli, preventing or reducing the expression of recombinant proteins and avoiding toxicity. When transcribed in K lactis, the intron is spliced from the mRNA allowing the translation of intact full- length, active recombinant gene product. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved}, language = {en} } @article{BeissenhirtzKwanKoetal.2004, author = {Beissenhirtz, Moritz Karl and Kwan, R. C. H. and Ko, K. M. and Renneberg, Reinhard and Scheller, Frieder W. and Lisdat, Fred}, title = {Comparing in vitro electrochemical measurement of superoxide scavenging activity with an in vivo assessment of antioxidant potential in Chinese tonifying herbs}, year = {2004}, abstract = {The in vitro superoxide scavenging activity (as determined by electrochemical measurement) and the in vivo antioxidant potential (as determined by a mouse model of carbon tetrachloride (CCl4) hepatotoxicity) of methanolic extracts prepared from 10 Chinese tonifying herbs were compared. Electrochemical measurement using a cytochrome c (Cyt. c) sensor showed that all of the tested herbal extracts exhibited a medium superoxide scavenging activity of different potency, as indicated by their IC50 values. The in vivo measurement demonstrated that 80\% of the herbal extracts displayed in vivo antioxidant potential, as assessed by the percentage of protection of the activity of plasma alanine aminotransferases and the hepatic glutathione regeneration capacity under CCl4-intoxicated condition. Although the in vitro antioxidant activity did not correlate quantitatively with the in vivo antioxidant potential, for 8 out of 10 samples a similar tendency was found. The rapid amperometric assessment of antioxidant potential by Cyt. c sensor may offer a convenient and direct method for screening as well as the quality control of herbal products. Copyright (C) 2004 John Wiley Sons, Ltd}, language = {en} } @article{BeissenhirtzSchellerLisdat2004, author = {Beissenhirtz, Moritz Karl and Scheller, Frieder W. and Lisdat, Fred}, title = {A superoxide sensor based on a multilayer cytochrome c electrode}, issn = {0003-2700}, year = {2004}, abstract = {A novel multilayer cytochrome c electrode for the quantification of superoxide radical concentrations is introduced. The electrode consists of alternating layers of cytochrome c and poly(aniline(sulfonic acid)) on a gold wire electrode. The formation of multilayer structures was proven by SPR experiments. Assemblies with 2-15 protein layers showed electrochemical communication with the gold electrode. For every additional layer, a substantial increase in electrochemically active cytochrome c (cyt. c) was found. For electrodes of more than 10 layers, the increase was more than 1 order of magnitude as compared to monolayer electrode systems. Thermodynamic and kinetic parameters of the electrodes were characterized. The mechanism of electron transfer within the multilayer assembly was studied, with results suggesting a protein-protein electron-transfer model. Electrodes of 2-15 layers were applied to the in vitro quantification of enzymatically generated superoxide, showing superior sensitivity as compared to a monolayer-based sensor. An electrode with 6 cyt. c/PASA layers showed the highest sensitivity of the systems studied, giving an increase in sensitivity of half an order of magnitude versus the that of the monolayer electrode. The stability of the system was optimized using thermal treatment, resulting in no loss in sensor signal or protein loading after 10 successive measurements or 2 days of storage}, language = {en} } @article{BistolasChristensonRuzgasetal.2004, author = {Bistolas, Nikitas and Christenson, A. and Ruzgas, T. and Jung, Christiane and Scheller, Frieder W. and Wollenberger, Ursula}, title = {Spectroelectrochemistry of cytochrome P450cam}, year = {2004}, abstract = {The spectroelectrochemistry of camphor-bound cytochrome P450cam (P450cam) using gold electrodes is described. The electrodes were modified with either 4,4'-dithiodipyridin or sodium dithionite. Electrolysis of P450cam was carried out when the enzyme was in solution, while at the same time UV visible absorption spectra were recorded. Reversible oxidation and reduction could be observed with both 4,4'-dithiodipyridin and dithionite modified electrodes. A formal potential (E-0') of -373 mV vs Ag/AgCl 1 M KCl was determined. The spectra of P450cam complexed with either carbon monoxide or metyrapone, both being inhibitors of P450 catalysis, clearly indicated that the protein retained its native state in the electrochemical cell during electrolysis. (C) 2003 Elsevier Inc. All rights reserved}, language = {en} } @article{FeulnerBielfeldtZachosetal.2004, author = {Feulner, Philine g. d. and Bielfeldt, Wiebke and Zachos, F. E. and Bradvarovic, J. and Eckert, I. and Hartl, G. B.}, title = {Mitochondrial DNA and microsatellite analyses of the genetic status of the presumed subspecies Cervus elaphus montanus (Carpathian red deer)}, issn = {0018-067X}, year = {2004}, abstract = {The possibly distinct Carpathian red deer was compared genetically to other European populations. We screened 120 red deer specimens from Serbia, the Romanian lowland and the Romanian Carpathians for genetic variability using 582 bp of the mitochondrial control region and nine polymorphic nuclear microsatellite loci. The study aimed at a population genetic characterization of the Carpathian red deer, which are often treated as a distinct subspecies (Cervus elaphus montanus). The genetic integrity of the Carpathian populations was confirmed through the haplotype distribution, private alleles and genetic distances. The Carpathian red deer are thus identified as one of the few remaining natural populations of this species, deserving special attention among game and conservation biologists. The history of the populations studied, in particular the introduction of Carpathian red deer into Romanian lowland areas in the 20th century, was reflected by the genetic data}, language = {en} } @article{Baumann2004, author = {Baumann, Otto}, title = {Spatial pattern of nonmuscle myosin-II distribution during the development of the Drosophila compound eye and implications for retinal morphogenesis}, year = {2004}, abstract = {Nonmuscle myosin-II is a motor protein that drives cell movement and changes in cell shape during tissue and organ development. This study has determined he dynamic changes in myosin-II distribution during Drosophila compound eye morphogenesis. In photoreceptor neurons, myosin-II is undetectable at the apical domain throughout the first half of pupal life, at which time this membrane domain is involuted into the epithelium and progresses toward the retinal floor. Myosin-II is deployed at the apical surface at about 60\% of pupal development, once the developing rhabdomeres reach the retinal floor. Subsequently, myosin-II becomes restricted to two stripes at the sides of the developing rhabdomere, adopting its final position within the visual cells R1-6; here, myosin-II is associated with a set of actin filaments that extend alongside the rhabdomeres. At the midpupal stage, myosin-II is also incorporated into stress-fiber-like arrays within the basal endfeet of the pigment cells that then change their shape. This spatiotemporal pattern of myosin- II localization and the morphological defects observed in the eyes of a myosin-II mutant suggest that the myosin-II/F- actin system is involved in the alignment of the rhabdomeres within the retina and in the flattening of the retinal floor. The observation that the myosin-II/F-actin arrays are incomplete or disorganized in R7/R8 and in rhodopsin-1-null R1-6 suggests further that the establishment and stability of this cytoskeletal system depend on rhodopsin-1 expression. (C) 2004 Elsevier Inc. All rights reserved}, language = {en} } @article{BaumannKuehnelDamesetal.2004, author = {Baumann, Otto and K{\"u}hnel, Dana and Dames, Petra and Walz, Bernd}, title = {Dopaminergic and serotonergic innervation of cockroach salivary glands : distribution and morphology of synapses and release sites}, year = {2004}, abstract = {The paired salivary glands in the cockroach are composed of acini with ion-transporting peripheral P-cells and protein-secreting central C-cells, and a duct system for the modification of the primary saliva. Secretory activity is controlled by serotonergic and dopaminergic neurons, whose axons form a dense plexus on the glands. The spatial relationship of release sites for serotonin and dopamine to the various cell types was determined by anti-synapsin immunofluorescence confocal microscopy and electron microscopy. Every C-cell apparently has only serotonergic synapses on its surface. Serotonergic and dopaminergic fibres on the acini have their release zones at a distance of similar to0.5 mum from the P-cells. Nerves between acinar lobules may serve as neurohaemal organs and contain abundant dopaminergic and few serotonergic release sites. Some dopaminergic and serotonergic release sites reside in the duct epithelium, the former throughout the duct system, the latter only in segments next to acini. These findings are consistent with the view that C-cells respond exclusively to serotonin, P-cells to serotonin and dopamine, and most duct cells only to dopamine. Moreover, the data suggest that C-cells are stimulated by serotonin released close to their surface, whereas P-cells and most duct cells are exposed to serotonin/dopamine liberated at some distance}, language = {en} } @article{ClermontWeddeSeitzetal.2004, author = {Clermont, A. and Wedde, M. and Seitz, V. and Podsiadlowski, L. and Lenze, D. and Hummel, M. and Vilcinskas, Andreas}, title = {Cloning and expression of an inhibitor of microbial metalloproteinases from insects contributing to innate immunity}, issn = {0264-6021}, year = {2004}, abstract = {The first IMPI (inhibitor of metalloproteinases from insects) was identified in the greater wax moth, Galleria mellonella [Wedde, Weise, Kopacek, Franke and Vilcinskas (1998) Eur. J. Biochem. 255, 535-543]. Here we report cloning and expression of a cDNA coding for this IMPI. The IMPI mRNA was identified among the induced transcripts from a subtractive and suppressive PCR analysis after bacterial challenge of G. mellonella larvae. Induced expression of the IMPI during a Immoral immune response was confirmed by real-time PCR, which documented up to 500 times higher amounts of IMPI mRNA in immunized larvae in comparison with untreated ones. The IMPI sequence shares no similarity with those of tissue inhibitors of metalloproteinases or other natural inhibitors of metalloproteinases, and the recombinant IMPI specifically inhibits thermolysin-like metalloproteinases, but not matrix metalloproteinases. These results support the hypothesis that the IMPI represents a novel type of immune-related protein which is induced and processed during the G. mellonella humoral immune response to inactivate pathogen-associated thermolysin-like metalloproteinases}, language = {en} } @article{BeathamRomeroTownsendetal.2004, author = {Beatham, Jane L. and Romero, Rosario and Townsend, Stuart K.M. and Hacker, Terry and VanderVen, Peter F. M. and Blanco, Gonzalo}, title = {Filamin C interacts with the muscular dystrophy KY protein and is abnormally distributed in mouse KY deficient muscle fibres}, issn = {0964-6906}, year = {2004}, abstract = {The KY protein has been implicated in a neuromuscular dystrophy in the mouse, but its role in muscle function remains unclear. Here, we show that KY interacts with several sarcomeric cytoskeletal proteins including, amongst others, filamin C and the slow isoform of the myosin-binding protein C. These interactions were confirmed in vitro and because of its central role in skeletal muscle disease, characterized in more detail for filamin C. A role for KY in regulating filamin C function in vivo is supported by the expression analysis of filamin C in the null ky mouse mutant, where distinct irregular subcellular localization of filamin C was found in subsets of muscle fibres, which appears to be a specific outcome of KY deficiency. Furthermore, KY shows protease activity in in vitro assays, and specific degradation of filamin C by KY is shown in transfected cells. Given the enzymatic nature of the KY protein, it is likely that some of the identified partners are catalytic substrates. These results suggest that KY is an intrinsic part of the protein networks underlying the molecular mechanism of several limb-girdle muscular dystrophies, particularly those where interactions between filamin C and disease causing proteins have been shown}, language = {en} } @article{GollGarciaMazuchAltmannetal.2004, author = {Goll-Garcia, D. and Mazuch, J. and Altmann, Thomas and M{\"u}ssig, Carsten}, title = {Exordium regulates brassinosteroid-responsive genes}, year = {2004}, abstract = {In a screen for potential mediators of brassinosteroid (BR) effects, the EXORDIUM (EXO) protein was identified as a regulator of BR-responsive genes. The EXO gene was characterized as a BR-up-regulated gene. EXO overexpression under the control of the 35SCaMV promoter resulted in increased transcript levels of the BR-up-regulated KCS1, Exp5, delta-TIP, and AGP4 genes, which likely are involved in the mediation of BR-promoted growth. 35S::EXO lines grown in soil or in synthetic medium showed increased vegetative growth in comparison to wild-type plants, resembling the growth phenotype of BR-treated plants. Thus, the EXO protein most likely promotes growth via the modulation of gene expression patterns. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved}, language = {en} } @article{JeltschHansenTackmannetal.2004, author = {Jeltsch, Florian and Hansen, Frank and Tackmann, K. and Staubach, C. and Thulke, Hans-Hermann}, title = {Processes leading to a spatial aggregation of Echinococcus multilocularis in its natural intermediate host Microtus arvalis}, year = {2004}, abstract = {The small fox tapeworm (Echinococcus multilocularis) shows a heterogeneous spatial distribution in the intermediate host (Microtus arvalis). To identify the ecological processes responsible for this heterogeneity, we developed a spatially explicit simulation model. The model combines individual-based (foxes, Vulpes vulpes) and grid- based (voles) techniques to simulate the infections in both intermediate and definite host. If host populations are homogeneously mixed, the model reproduces field data for parasite prevalence only for a limited number of parameter combinations. As ecological parameters inevitably vary to a certain degree, we discarded the homogeneous mixing model as insufficient to gain insight into the ecology of the fox tapeworm cycle. We analysed five different model scenarios, each focussing on an ecological process that might be responsible for the heterogeneous spatial distribution of E multilocularis in the intermediate host. Field studies revealed that the prevalence ratio between intermediate and definite host remains stable over a wide range of ecological conditions. Thus, by varying the parameters in simulation experiments, we used the robustness of the agreement between field data and model output as quality criterion for the five scenarios. Only one of the five scenarios was found to reproduce the prevalence ratio over a sufficient range of parameter combinations. In the accentuated scenario most tapeworm eggs die due to bad environmental conditions before they cause infections in the intermediate host. This scenario is supported by the known sensitivity of tapeworm eggs to high temperatures and dry conditions. The identified process is likely to lead to a heterogeneous availability of infective eggs and thus to a clumped distribution of infected intermediate hosts. In conclusion, areas with humid conditions and low temperatures must be pointed out as high risk areas for human exposure to E. multilocularis eggs as well. (C) 2004 on behalf of Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved}, language = {en} } @article{HeinkenZippel2004, author = {Heinken, Thilo and Zippel, Elke}, title = {Natural re-colonization of experimental gaps by terricolous bryophytes in Central European pine forests}, issn = {0029-5035}, year = {2004}, abstract = {In northeastern German pine forests we studied re-colonization patterns of experimental gaps by four dominant bryophyte species (Dicranum scoparium, Hypnum jutlandicum, Pleurozium schreberi and Scleropodium purum) over three years. Both vegetation and litter layer were removed on 1 m(2) plots within +/- pure colonies of the experimental species, while the humus layer was left intact. All plots were vegetatively re-colonized by the species which was dominant before gap creation. Three mechanisms of re-colonization occurred and interacted: (1) advance of surrounding shoots from the edge into the gaps by clonal growth, (2) dispersal of detached single shoots as well as larger clumps of multiple shoots into the plots, resulting in new colonies by continuing growth, and (3) regeneration from a soil diaspore bank consisting of seemingly dead stem fragments in the humus layer of the gaps. Scleropodium purum, which occurs at locations with good water and nutrient supply, displayed the most rapid growth. Here, some plots were completely recovered after three years. Despite lower rates of advance from the edge, colonization of Hypnum jutlandicum was faster than and of Dicranum scoparium as fast than that of Pleurozium schreberi because of a larger diaspore bank. Thus, each bryophyte species was characterized by a different habitat occupation strategy. The different clonal colonization strategies account for the high competitive capacity and regeneration potential of the investigated bryophyte species in pine forests despite of the lack of generative reproduction. Experimental disturbance resulted in a temporary increase of bryophyte diversity, because short-lived Colonists with a low competitive capacity colonized the gaps, before they will be overgrown by the dominant Perennials}, language = {en} } @article{KulysKrikstopaitisSchelleretal.2004, author = {Kulys, J. and Krikstopaitis, K. and Scheller, Frieder W. and Wollenberger, Ursula}, title = {Electrochemical parameters of phenoxazine derivatives in solution and at monolayer-modified gold electrodes}, year = {2004}, abstract = {Electrochemical properties of beta-(10-phenoxazinyl) propylamine (APPX) and beta-(10-phenoxazinyl) propionic acid (PPX) have been studied in solution, and in immobilized state on gold electrodes modified with monolayers of cystamine and mercaptoundecanoic acid. A reversible diffusion-controlled process of APPX and PPX was observed at a bare gold electrode. The electrochemical conversion of both compounds at modified gold electrodes was a quasireversible diffusion-controlled process. The redox potential of immobilized APPX (443 mV) was similar to the potential in solution, while the value of the immobilized PPX was 131 mV higher than in solution. The immobilized mediators were electrocatalytically active in the fungal peroxidase-catalyzed hydrogen peroxide reduction}, language = {en} } @article{DamesSchmidtWalzetal.2004, author = {Dames, Petra and Schmidt, R. and Walz, Bernd and Baumann, Otto}, title = {Regulation of vacuolar-type H+-ATPase (vATPase) in blowfly salivary glands}, issn = {0171-9335}, year = {2004}, language = {en} }